CN208898893U - Microbial association culture apparatus - Google Patents
Microbial association culture apparatus Download PDFInfo
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- CN208898893U CN208898893U CN201821363320.3U CN201821363320U CN208898893U CN 208898893 U CN208898893 U CN 208898893U CN 201821363320 U CN201821363320 U CN 201821363320U CN 208898893 U CN208898893 U CN 208898893U
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- culture chamber
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- chamber
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Abstract
A kind of microbial association culture apparatus, comprising: the first cabinet surrounds the first culture chamber for being suitable for cultivating microorganism；Second cabinet is arranged in the first culture chamber, and the second cabinet surrounds the second culture chamber for being isolated with the first culture chamber and being suitable for cultivating microorganism；Second cabinet is suitable for shrinking when the pressure of the first culture chamber is greater than the pressure of the second culture chamber, to increase the pressure of the second culture chamber；The first culture chamber pressure less than the second culture chamber pressure when expand, with reduce the second culture chamber pressure.On the one hand, can prevent the first culture chamber, in the second culture chamber substance exchange, so that the first culture chamber, the second culture chamber is cultivated different microorganisms simultaneously, to reduce cost.On the other hand, can be by the gas pressure intensity in the first culture chamber of control, to control the gas pressure intensity in the second culture chamber, when the space of the second culture chamber is too small, the pressure of gas, meets test requirements document in the second culture chamber of control that also can be relatively stable.
Description
Technical field
The utility model relates to field of microbial culture technology, and in particular to a kind of microbial association culture apparatus.
Background technique
Microculture mainly includes the culture to virus, bacterium, fungi.The growth of microorganism needs specific environment,
Including temperature, pressure, pH value and nutrient solution etc..In addition, in air the ingredient of gas with various also can largely influence it is micro-
The growth of biology.In the prior art, usually using culture apparatus to cultivate microorganism, the growth ring as needed for different microorganisms
Border is different, and a kind of culture apparatus is typically only capable to cultivate a kind of microorganism.Therefore, when needing while cultivating multiple-microorganism,
It can only be by making multiple culture apparatus to cultivate different microorganisms respectively, this will lead to the rising of cost.Also, it is different micro-
Space needed for biological culture is simultaneously different, when it is required culture microorganism culture apparatus space it is too small when, then can be difficult to compared with
The pressure in culture apparatus is accurately adjusted, causes experimental situation unstable.
Utility model content
The utility model solves the problems, such as it is that the culture apparatus of the prior art can not cultivate multiple-microorganism simultaneously, causes micro-
Biological culture increased costs.
To solve the above problems, the utility model provides a kind of microbial association culture apparatus, comprising: the first cabinet encloses
At the first culture chamber for being suitable for cultivating microorganism；Second cabinet is arranged in first culture chamber, and second cabinet surrounds
The second culture chamber of microorganism is isolated and is suitable for cultivating with first culture chamber；Second cabinet is suitable for described the
The pressure of one culture chamber is shunk when being greater than the pressure of second culture chamber, to increase the pressure of second culture chamber；Institute
State the first culture chamber pressure be less than second culture chamber pressure when expansion, to reduce the pressure of second culture chamber.
Optionally, second cabinet includes shell and the elastic membrane for being set to the shell, the shell and the bullet
Property film surround second culture chamber, the elastic membrane is suitable for occurring elastic deformation so that the second cabinet contraction or expansion.
Optionally, second cabinet includes shell and the piston for being set to the shell, the shell and the piston
Second culture chamber is surrounded, the piston is suitable for the relatively described shell and moves back and forth so that second cabinet contraction or swollen
It is swollen.
Optionally, the outer surface of the elastic membrane, inner surface wherein at least one surface on be coated with heat-barrier material；
Or, the elastic membrane is made of heat-barrier material.
Optionally, the outer surface of the piston, inner surface wherein at least one surface be equipped with thermal insulation layer；Or, described
Piston is made of heat-barrier material.
Optionally, second cabinet at least two.
Optionally, first cabinet, which is equipped with, is connected to first culture chamber and the first extraneous air inlet pipe and first row
Tracheae, second cabinet, which is equipped with, is connected to second culture chamber and the second extraneous air inlet pipe and second exhaust pipe.
Optionally, the first electric heating wire is equipped in first culture chamber；And/or the is equipped in second culture chamber
Two electric heating wires.
Optionally, the first sensor for being adapted to detect for temperature and pressure is equipped in first culture chamber；And/or it is described
The second sensor for being adapted to detect for temperature and pressure is equipped in second culture chamber.
Optionally, the first nutrition liquid bath is additionally provided in first culture chamber, the first nutrition liquid bath passes through the first pipe
Road connection is extraneous；And/or the second nutrition liquid bath is additionally provided in second culture chamber, the second nutrition liquid bath passes through second
Pipeline connection is extraneous.
Compared with prior art, the technical solution of the utility model has the advantage that
By the way that the second cabinet is arranged in the first culture chamber that the first cabinet is surrounded, the second cabinet surrounds the second culture
Chamber makes first the first culture chamber of cabinet and the second culture chamber, it is thus possible to prevent the first culture chamber, substance in the second culture chamber
Exchange, enables the first culture chamber, the second culture chamber to cultivate different microorganisms simultaneously, to reduce cost.Also, the first case
Body can according to the size expansion or shrinkage of gas pressure intensity in the first culture chamber, to change the gas pressure intensity in the second culture chamber,
It thus can be by the gas pressure intensity in the first culture chamber of control, to control the gas pressure intensity in the second culture chamber, when the second training
When the space of feeding chamber is too small, the pressure of gas, meets test requirements document in the second culture chamber of control that also can be relatively stable.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model first embodiment microbial association culture chamber；
Fig. 2 is the structural schematic diagram of the utility model second embodiment microbial association culture chamber.
Specific embodiment
It is understandable to enable the above objects, features, and advantages of the utility model to become apparent, with reference to the accompanying drawing to this
The specific embodiment of utility model is described in detail.
First embodiment
Referring to Fig.1, a kind of microbial association culture apparatus 100, including the first cabinet 10, the first cabinet 10, which surrounds, is suitable for training
Support the first culture chamber 10a of microorganism.Wherein, the second cabinet 20 is equipped in the first culture chamber 10a, the second cabinet 20, which surrounds, to be suitable for
The second culture chamber 20a of microorganism is cultivated, and the second cabinet 20 completely cuts off the first culture chamber 10a and the second culture chamber 20a, to prevent
The exchange of substance in first culture chamber 10a, the second culture chamber 20a.The growing environment as needed for different microorganisms is not identical,
Different microorganisms can be cultivated simultaneously in the first culture chamber 10a, the second culture chamber 20a being isolated, to reduce cost.
In the present embodiment, the second cabinet 20 includes shell 21a and the elastic membrane 22a, shell 21a, bullet that are set to shell 21a
Property film 22a surrounds the second culture chamber 20a.Elastic membrane 22a is suitable for that elastic deformation occurs.
Specifically, when the gas pressure intensity in the first culture chamber 10a is greater than the gas pressure intensity in the second culture chamber 20a, bullet
Property film 22a deformation occurs that the second cabinet 20 is made to shrink (as shown in Fig. 1 dotted line aa), the space of the second culture chamber 20a reduces, pressure
Increase.Until when the gas pressure intensity in the second culture chamber 20a is equal or close to the gas pressure intensity in the first culture chamber 10a, bullet
Property film 22a stopping deform, so that the space size of the second culture chamber 20a and gas pressure intensity is maintained relatively stable state.
When the gas pressure intensity in the first culture chamber 10a is less than gas pressure intensity in the second culture chamber 20a, elastic membrane 22a
Deformation occurs makes the second cabinet 20 expand (as shown in Fig. 1 dotted line bb), and the space of the second culture chamber 20a increases, and pressure reduces.Directly
When into the second culture chamber 20a, the pressure of gas is equal or close to the pressure of gas in the first culture chamber 10a, elastic membrane 22a
Stopping deforms, and the space size of the second culture chamber 20a and gas pressure intensity is made to maintain relatively stable state.
That is, the microbial association culture apparatus 100 of the present embodiment can pass through gas in the first culture chamber 10a of control
The pressure of body, to control the pressure of gas in the second culture chamber 20a.As previously mentioned, space needed for different microorganisms culture is simultaneously
Different, the culture of specified microorganisms needs specific environment to need to have the second culture chamber 20a sometimes to reduce cost
Very small space can then be difficult to accurately directly adjust the second culture chamber when the space of the second culture chamber 20a is too small
The pressure of gas, unstable so as to cause experimental situation in 20a.By adjusting the pressure of gas in the first culture chamber 10a, then can
The pressure of gas, meets test requirements document in enough relatively stable the second culture chamber of control 20a.
In the present embodiment, shell 21a is specially heat insulation shell, and elastic membrane 22a is specifically made of heat-barrier material.Therefore,
Heat exchange should not be generated between one culture chamber 10a, the second culture chamber 20a so that the first culture chamber 10a, the second culture chamber 20a it
Between have different temperature.
That is, first culture chamber 10a, the second culture chamber 20a are with identical big in microbial association culture apparatus 100
Gas pressure intensity that is small or being close, but there is different temperature and different gaseous environments.First culture chamber 10a, the second culture
Chamber 20a is suitable for while cultivating that gas pressure intensity is close but the variety classes of other environmental conditions inconsistent (temperature, gaseous species etc.)
Microorganism.
It should be appreciated that in order to make the second cabinet 20 that there is better heat insulation, it can also be in the inner surface of elastic membrane 22a
Upper coating heat-barrier material, or heat-barrier material is coated on the outer surface of elastic membrane 22a.
With continued reference to Fig. 1, set in the first culture chamber 10a there are two the second cabinet 20, two the second cabinets 20 are respectively enclosed to
Two the second culture chamber 20a.Wherein, two the second cabinets 20 are independently arranged, the first culture chamber 10a and two second cultures
Substance in chamber 20a can not swap.Therefore, different microorganisms can be cultivated simultaneously in two the second culture chamber 20a, make
Three kinds of different microorganisms can be cultivated simultaneously by obtaining microbial association culture apparatus 100, be further reduced the cost.
In other variations, more second cabinets 20 can also be set in the first culture chamber 10a, to cultivate simultaneously
More different types of microorganisms.It should be noted that the design of multiple second cabinets 20 can be cross-referenced, the present embodiment tool
Body is specifically described with one of them second cabinet 20.
As shown in Figure 1, being equipped with the first nutrition liquid bath 11 in the first culture chamber 10a, the first nutrition liquid bath 11 is suitable for cultivating micro-
Biology.First nutrition liquid bath 11, which has, is connected to the first extraneous pipeline 11a, and nutrient solution is suitable for through the first pipeline 11a from outer
Boundary is discharged into the first nutrition liquid bath 11；Waste liquid in the first nutrition liquid bath 11 can also be arranged by the first pipeline 11a
It puts to the external world.
Likewise, being equipped with the second nutrition liquid bath 23 in the second culture chamber 20a, the second nutrition liquid bath 23 is suitable for cultivating micro- life
Object.Second nutrition liquid bath 23, which has, is directly connected to the second extraneous pipeline 23a, nutrient solution be suitable for by the second pipeline 23a from
The external world is discharged into the second nutrition liquid bath 23；Waste liquid in the second nutrition liquid bath 23 can also pass through the second pipeline 23a
It is emitted into the external world.
In the present embodiment, be additionally provided on the first cabinet 10 the first culture chamber 10a of connection and the first extraneous air inlet pipe 12a and
Downtake pipe 12b, suitable in the first nutrition liquid bath 11 microorganism grow gas can be discharged by the first air inlet pipe 12a to
First culture chamber 10a, microorganism, which grows discharged gas, to be discharged outside to by downtake pipe 12b.In addition, due to
First cabinet 10 be it is non-deformable, can also pass through control the first air inlet pipe 12a air inflow and downtake pipe 12b row
Tolerance, to adjust the pressure in the first culture chamber 10a.Since the pressure in the second culture chamber 20a depends on the first culture chamber
10a, that is to say, that can be by controlling the air inflow of the first air inlet pipe 12a and the capacity of downtake pipe 12b to adjust the
Pressure in two culture chamber 20a.
Likewise, the second cabinet 20 is equipped with the second air inlet pipe 24a and the in directly the second culture chamber 20a of connection and the external world
Two exhaust pipe 24b, the gas grown suitable for microorganism in the second nutrition liquid bath 23 can be discharged into the by the second air inlet pipe 24a
One culture chamber 20a, microorganism, which grows discharged gas, to be discharged outside to by second exhaust pipe 24b.
As previously mentioned, growing environment needed for different microorganisms is not identical.Due to the first culture of the second cabinet 20 isolation
Chamber 10a and the second culture chamber 20a so that convection current will not be generated between the first culture chamber 10a and the second culture chamber 20a, first into
Tracheae 12a and the second air inlet pipe 24a can be discharged into different gas, to adapt to the growth demand of different microorganisms.
As shown in Figure 1, being additionally provided with the first electric heating wire 13 in the first culture chamber 10a, the first electric heating wire 13 is for heating
First culture chamber 10a enables to adapt to be located at micro- life in the first nutrition liquid bath 11 to promote the temperature of the first culture chamber 10a
The growth of object.The second electric heating wire 25 is additionally provided in second culture chamber 20a, the second electric heating wire 25 is for heating the second culture chamber
20a enables the growth for adapting to be located at microorganism in the second nutrition liquid bath 23 to promote the temperature of the second culture chamber 20a.
Since shell 21a is heat insulation shell, elastic membrane 22a is made of heat-barrier material.Therefore, it can use the first electric heating
First culture chamber 10a, the second culture chamber 20a are heated to different temperature respectively by the 13, second electric heating wire 25 of silk, to adapt to not
With the growth demand of microorganism.
In order to grasp the temperature of the first culture chamber 10a and the temperature of the second culture chamber 20a, the first culture chamber in real time
It is additionally provided with first sensor 14 in 10a, temperature and pressure of the first sensor 14 to the first culture chamber of real-time detection 10a, with
Guarantee the growth conditions of microorganism in the first nutrition liquid bath 11.Second sensor 26 is additionally provided in second culture chamber 20a, second passes
Temperature and pressure of the sensor 26 to the second culture chamber of real-time detection 20a, to guarantee the life of microorganism in the second nutrition liquid bath 23
Elongate member.
Second embodiment
Referring to Fig. 2, the present embodiment and first embodiment the difference is that: the second cabinet 20 of first embodiment includes
Shell 21a and elastic membrane 22a, shell 21a and elastic membrane 22a surround the second culture chamber 20a；And second cabinet 20 of the present embodiment
Including shell 21b and piston 22b, shell 21b and piston 22b surround the second culture chamber 20a.
Specifically, shell 21b has bottom wall 21m and the side wall 21n around bottom wall 21m, shell 21b with bottom wall 21m's
Opening (not identifying in figure) is formed at relative position.Piston 22b is arranged at aperture position, and the relative position bottom wall 21m, and with
Sealing is formed between side wall 21n.
When the pressure of gas in the first culture chamber 10a is greater than the pressure of gas in the second culture chamber 20a, piston 22b court
It is moved to the direction of bottom wall 21m, the contraction of the second cabinet 20 reduces the space of the second culture chamber 20a, and pressure increases.Until second
When the pressure of gas is equal or close to the pressure of gas in the first culture chamber 10a in culture chamber 20a, piston 22b stop motion,
The space size and gas pressure intensity for making the second culture chamber 20a maintain relatively stable state.
When the pressure of gas in the first culture chamber 10a is less than the pressure of gas in the second culture chamber 20a, piston 22b court
It is moved backwards to the direction of bottom wall 21m, the expansion of the second cabinet 20 increases the space of the second culture chamber 20a, and pressure reduces.Until the
When the pressure of gas is equal or close to the pressure of gas in the first culture chamber 10a in two culture chamber 20a, piston 22b stops fortune
It is dynamic, so that the space size of the second culture chamber 20a and gas pressure intensity is maintained relatively stable state.
Therefore, by adjusting the pressure of gas in the first culture chamber 10a, then the second culture chamber of control that can be relatively stable
The pressure of gas, meets test requirements document in 20a.
In the present embodiment, shell 21b is specially heat insulation shell, and piston 22b is equipped with heat-insulated backwards to the outer surface of bottom wall 21m
The thermal insulation layer 22m that material is formed.Therefore, heat exchange should not be generated between the first culture chamber 10a, the second culture chamber 20a, so that the
There is different temperature between one culture chamber 10a, the second culture chamber 20a.It should be appreciated that thermal insulation layer 22m can not only be provided at
The inner surface in piston 22b also can be set in the outer surface of piston 22b.Piston 22b itself can also be made by heat-barrier material system
At so that the second cabinet 20 has better heat insulation.
Although the utility model discloses as above, the utility model is not limited to this.Anyone skilled in the art,
It does not depart from the spirit and scope of the utility model, can make various changes or modifications, therefore the protection scope of the utility model
It should be defined by the scope defined by the claims..
Claims (10)
1. a kind of microbial association culture apparatus characterized by comprising
First cabinet surrounds the first culture chamber for being suitable for cultivating microorganism；
Second cabinet, be arranged in first culture chamber, second cabinet surround be isolated with first culture chamber and
Suitable for cultivating the second culture chamber of microorganism；
Second cabinet is suitable for shrinking when the pressure of first culture chamber is greater than the pressure of second culture chamber, to increase
The pressure of big second culture chamber；It is expanded when the pressure of first culture chamber is less than the pressure of second culture chamber,
To reduce the pressure of second culture chamber.
2. microbial association culture apparatus as described in claim 1, which is characterized in that second cabinet includes shell and sets
It is placed in the elastic membrane of the shell, the shell and the elastic membrane surround second culture chamber, and the elastic membrane is suitable for hair
Raw elastic deformation is so that the second cabinet contraction or expansion.
3. microbial association culture apparatus as described in claim 1, which is characterized in that second cabinet includes shell and sets
It is placed in the piston of the shell, the shell and the piston surround second culture chamber, and the piston is suitable for relatively described
Shell moves back and forth so that the second cabinet contraction or expansion.
4. microbial association culture apparatus as claimed in claim 2, which is characterized in that the outer surface of the elastic membrane, interior table
Heat-barrier material is coated on the wherein at least one surface in face；Or, the elastic membrane is made of heat-barrier material.
5. microbial association culture apparatus as claimed in claim 3, which is characterized in that the outer surface of the piston, inner surface
Wherein at least one surface be equipped with thermal insulation layer；Or, the piston is made of heat-barrier material.
6. microbial association culture apparatus as described in any one in claim 1-5, which is characterized in that second cabinet is extremely
It is less two.
7. microbial association culture apparatus as described in any one in claim 1-5, which is characterized in that on first cabinet
Equipped with first culture chamber and the first extraneous air inlet pipe and downtake pipe is connected to, second cabinet is equipped with connection institute
State the second culture chamber and the second extraneous air inlet pipe and second exhaust pipe.
8. microbial association culture apparatus as described in any one in claim 1-5, which is characterized in that first culture chamber
Inside it is equipped with the first electric heating wire；And/or the second electric heating wire is equipped in second culture chamber.
9. microbial association culture apparatus as described in any one in claim 1-5, which is characterized in that first culture chamber
It is interior to be equipped with the first sensor for being adapted to detect for temperature and pressure；And/or second culture chamber in be equipped be adapted to detect for temperature and
The second sensor of pressure.
10. microbial association culture apparatus as described in any one in claim 1-5, which is characterized in that first culture chamber
It is inside additionally provided with the first nutrition liquid bath, the first nutrition liquid bath is extraneous by the first pipeline connection；And/or second culture
Intracavitary to be additionally provided with the second nutrition liquid bath, the second nutrition liquid bath is extraneous by the second pipeline connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821363320.3U CN208898893U (en) | 2018-08-23 | 2018-08-23 | Microbial association culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821363320.3U CN208898893U (en) | 2018-08-23 | 2018-08-23 | Microbial association culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208898893U true CN208898893U (en) | 2019-05-24 |
Family
ID=66569963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821363320.3U Active CN208898893U (en) | 2018-08-23 | 2018-08-23 | Microbial association culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208898893U (en) |
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2018
- 2018-08-23 CN CN201821363320.3U patent/CN208898893U/en active Active
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