CN208883906U - Medical experiment cell culture apparatus - Google Patents

Abstract

The utility model discloses a kind of medical experiment cell culture apparatus, comprising: culture cabinet, case lid, culturing rack and culture solution import and export mechanism, and culture cabinet includes internal box and is set to the outer case of internal box peripheral side around internal box；Internal box has a hollow cavity and is set as the opening of corresponding internal box bottom at the top of hollow cavity；It is formed between outer case and internal box at the top of toroidal cavity and outer case and is set as closed plate；The aperture for being suitable for being connected to internal box and outer case is equipped in the side wall of internal box；Tapping is provided with filtering tissue.Case lid is suitable for being covered on the opening of internal box.Culturing rack is suitable for being placed in the hollow cavity of culture cabinet for carrying cell.Culture solution imports and exports mechanism, goes out liquid component, and the feed liquor component suitable for being connected to by case lid with hollow cavity including be suitable for being connected to by the closed plate of outer case with toroidal cavity.

Description

Medical experiment cell culture apparatus

Technical field

The utility model relates to medical experiment equipment technical fields more particularly to a kind of medical experiment to be filled with cell culture It sets.

Background technique

In hospital's experiment, cell culture is more common one kind in laboratory facilities, needs to make in cell cultivation process With culture solution, since cell can generate metabolin during the cultivation process, metabolin can have an impact the ingredient of culture solution, thus The quality of culture solution is reduced, so, it needs to replace culture solution in cell cultivation process, existing many cell culture are set In standby when needing to replace culture solution, generally require to shift cell, such as intracorporal to incubator is placed in Culture plate or culture dish are transferred out of culture cabinet, in this way, it is not only cumbersome, but also suddenly to thin during cell culture Born of the same parents shift, it is understood that there may be the problem of leading to cell death due to the difference of the temperature of incubator inside and outside.

In most cell cultivation equipments, the supply pipe for culture solution is mostly direct insertion to addition training in incubator body Nutrient solution, and for whole culture cabinet in order to keep the metastable environment of cell culture, culture cabinet will not be carried out into Row rocks, in this way, more equably real for carrying out in incubator body for the intracorporal culture solution difficulty or ease realization of incubator is added The supply of existing culture solution is often easy when culture solution supply is uneven so as to progress culture solution in incubator body During addition, it will cause newly added culture solution and more concentrate on some region, so cultivate box house for whole Cell for, the uniform growing environment of balance can not be formed, error will cause for the observation of the cell in later period.

On the other hand, mostly culture plate and culture dish etc. usually used in cell cultivation equipment in the prior art are flat The cell bearing structure of hardened structure, such structure problem unlimited there is only the cell quantity that can be cultivated, and be easy Existing cell stacks phenomenon, is unfavorable for building the environment of uniform growth to cell.

Utility model content

The purpose of the utility model is to provide a kind of medical experiment cell culture apparatus, to solve to improve in the confined space Cell culture quantity and replacement culture solution can be to avoid metastatic cells culturing rack the technical issues of.

The medical experiment of the utility model is achieved in that with cell culture apparatus

A kind of medical experiment cell culture apparatus, comprising:

Cabinet is cultivated, the culture cabinet includes internal box and is set to the outer of internal box peripheral side around the internal box Cabinet；The internal box has a hollow cavity and is set as corresponding to opening for the internal box bottom at the top of hollow cavity Mouthful；It is formed between the outer case and internal box at the top of toroidal cavity and the outer case and is set as closed plate；Described The side wall of internal box is equipped with the aperture for being suitable for being connected to the internal box and outer case；The tapping is provided with filtering tissue；

Case lid, the case lid are suitable for being covered on the opening of the internal box；

Culturing rack, the culturing rack are suitable for being placed in the hollow cavity of the culture cabinet for carrying cell；It is described Culturing rack includes the fibrous framework layer of multiple stackings；And

Culture solution imports and exports mechanism, including be suitable for being connected to by the closed plate of the outer case with the toroidal cavity Liquid component out, and the feed liquor component suitable for being connected to by case lid with the hollow cavity.

In the utility model preferred embodiment, it is described go out liquid component include being adapted for insertion into the toroidal cavity and close The outlet tube of outer case bottom end, be connected with the outlet tube go out liquid peristaltic pump, and by go out liquid connecting tube and it is described go out The connected eluate collection case of liquid peristaltic pump.

In the utility model preferred embodiment, the feed liquor component includes crossbearer between the case lid two sidewalls At least two throwing liquid pipes；

Two both ends for throwing liquid pipe are respectively connected with to be respectively formed feed liquor first end and feed liquor second end；It is described into The hollow structures that liquid first end is and two throwing liquid pipe inner cavities penetrate through；The feed liquor second end be and two throwing liquid pipes The solid construction of inner cavity isolation；

The feed liquor first end runs through the side wall of the case lid to be connected with the feed liquor peristaltic pump outside case lid；The feed liquor is compacted Dynamic pump is connect by feed liquor connecting tube with feed liquor cabinet；And

It is described to throw permeability pipeline made of the permselective membrane that liquid pipe is porous structure.

In the utility model preferred embodiment, the fibrous framework layer has a plurality of fiber filament, a plurality of fiber Silk is arranged in parallel；And

The fiber filament of fibrous framework layer described in every adjacent two layers is staggered；

It is formed between adjacent two fiber filaments being located on the same floor between cell attachment growth and culture solution circulation Gap.

In the utility model preferred embodiment, the number of plies of the fibrous framework layer is 4-8 layers；And

The size in the gap is 250-350 μm.

In the utility model preferred embodiment, the filtering tissue includes the pillar for the double medium filtration film being arranged inside Hollow cylinder and the hollow connector being integrally connected with the pillar hollow cylinder；

The outside of the hollow connector be equipped with external screw thread, and the internal box aperture hole wall be equipped be suitable for The internal screw thread of the external screw thread adaptation of hollow connector；

The filter opening aperture of the double medium filtration film is sequentially reduced by the direction of internal box towards outer case.

In the utility model preferred embodiment, the aperture of the internal box includes opening up in the side wall of the internal box At least a pair of of aperture；And

A pair of aperture is all set in the bottom position of nearly internal box on the side wall of the internal box.

In the utility model preferred embodiment, a gas vent is offered at the center of the case lid；

Ventilative bacteriological filtration film is covered at the gas vent.

In the utility model preferred embodiment, the bottom of the internal box is additionally provided with one and is suitable for adding internal box The heating plate of heat；The internal box is made of Heat Conduction Material.

In the utility model preferred embodiment, the bottom of the hollow cavity of the internal box is equipped with more pillars；It is more Pillar described in root is equal relative to the height of the hollow cavity bottom；And the culturing rack is supported in the more pillars On.

Using above-mentioned technical proposal, the utility model has the following beneficial effects: the medical experiment of the utility model is used Cell culture apparatus, the culture cabinet of design include internal box and outer case, import and export mechanism in conjunction with culture solution, can make When needing replacing culture solution, the culturing rack for not needing to carry cell removes culture cabinet, can train to avoid due to transfer The generation of the problem of cell damage that feeding frame is likely to occur.Further through the culturing rack phase of the fibrous framework layer of multiple stackings of use It for the culture dish and culture plate of slab construction, may be implemented to cultivate cell in three-dimensional space, reduce cell and stack now The generation of elephant, and cell culturing surfaces product is dramatically increased under the conditions of not increasing spatial volume, cell Proliferation amount and two dimension are flat Face culture compare, can several times even tens times growth, can be realized the large-scale culture of cell, meet mass propgation cell Use demand.

Further, feed liquor component includes at least two piece throwing liquid pipes of the crossbearer between case lid two sidewalls, so throwing liquid Structure compared to the prior art in single plug-in type be plugged in culture box house structure for, can to culture cabinet During interior supplement culture solution, supply to balancedly culture solution is carried out in incubator avoids point of newly added culture solution Cloth the problems in excessively collects, and builds the balanced growing environment of cell in incubator body.

Further, for the utility model relates to filtering tissue using be threadedly coupled by the way of with internal box phase Even, in this way, being conveniently replaceable different filtering tissues during being cultivated for different size of cell, playing cell With the centrifugation of culture solution, the integrally-built scope of application of the utility model is substantially increased.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of the medical experiment cell culture apparatus of the utility model；

Fig. 2 is the structural schematic diagram of the throwing liquid pipe of the medical experiment cell culture apparatus of the utility model；

Fig. 3 is the structural schematic diagram of the culture cabinet of the medical experiment cell culture apparatus of the utility model；

Fig. 4 is the structural schematic diagram of the filtering tissue of the medical experiment cell culture apparatus of the utility model；

Fig. 5 is the structural schematic diagram of the culturing rack of the medical experiment cell culture apparatus of the utility model.

In figure: culture cabinet 100, outer case 101, internal box 102, hollow cavity 103, toroidal cavity 104, aperture 105, Be open 106, L shape corner 108, pillar 110, case lid 200, bayonet 201, wears groove 203, culturing rack 300, fiber filament 302, gap 303, outlet tube 401, out liquid peristaltic pump 402, out liquid connecting tube 403, eluate collection case 405, throwing liquid pipe 501, feed liquor first end 502, feed liquor second end 503, feed liquor peristaltic pump 505, feed liquor cabinet 506, filter membrane 601, hollow connector 602, pillar are hollow Cylinder 603, gas vent 701, ventilative bacteriological filtration film 702, heating plate 800.

Specific embodiment

In order to make the content of the utility model be easier to be clearly understood, below according to specific embodiment and combine attached Figure, is described in further detail the utility model.

It please refers to shown in 1, present embodiments provides a kind of medical experiment cell culture apparatus, comprising: culture cabinet 100, case lid 200, culturing rack 300 and culture solution import and export mechanism.It should be noted that the medical experiment of the present embodiment design With cell culture apparatus especially suitable for aerobic in incubation but be not that especially aerobic cell uses, i.e., for the confession of oxygen It is used to the culture for a kind of cell for not needing especially to be regulated and controled.

Cultivate the outer case that cabinet 100 includes internal box 102 and is set to 102 peripheral side of internal box around internal box 102 101；Internal box 102 has a hollow cavity 103 and is set as corresponding 102 bottom of internal box at the top of hollow cavity 103 Opening；The top that toroidal cavity 104 and outer case 101 are formed between outer case 101 and internal box 102 is set as closing Plate；The aperture 105 for being suitable for being connected to internal box 102 and outer case 101 is equipped in the side wall of internal box 102；It is provided at aperture 105 Filter tissue.Case lid 200 is suitable for being covered at the opening 106 of internal box 102.Hollow cavity 103 is used to prevent carrying cell Cell culture rack 300 and receiving culture solution, toroidal cavity 104 are used for the circulation of culture solution.

The case where for the ease of from cell and culture solution in incubator external observation internal box 102, can set on case lid 200 There is a transparent observation panel (being not marked in figure).

It should be noted that can be set to flush arrangement for the top end face of outer case 101 and internal box 102, may be used also It is set as, the at L-shaped corner 108 of shape, the present embodiment attached drawing between the top end face of internal box 102 and the top end face of outer case 101 Only in case where being provided with L shape corner 108, for being provided with L shape corner 108 herein, can contribute to case lid 200 with it is interior During at the opening 106 of cabinet 102, the side wall of corresponding internal box 102 can be formed for case lid in L shape corner 108 200 guiding role, after the lid of case lid 200 closes at the opening 106 of internal box 102, case lid 200 is towards the side of outer case 101 Edge is equipped in the closed plate of outer case 101.

Culturing rack 300 is suitable for being placed in the hollow cavity 103 of culture cabinet 100 for carrying cell；It is adopted in this implementation Culturing rack 300 includes the fibrous framework layer of multiple stackings.

It please refers to shown in Fig. 5, specifically, fibrous framework layer has a plurality of fiber filament 302, a plurality of fiber filament 302 is set in parallel It sets；And the fiber filament 302 of every adjacent two layers fibrous framework layer is staggered；Adjacent two fiber filaments 302 being located on the same floor Between formed for cell attachment growth and culture solution circulation gap 303.The shape of fibrous framework layer is cylindrical body or polygon Cylinder.By taking the attached drawing of the utility model as an example, the fiber filament 302 of adjacent two layers fibrous framework layer is vertically arranged, this set side Formula is more convenient for observing.

The above-mentioned fibrous framework layer being made of fiber filament 302, common used material is PS (polyphenyl second in cell culture at present Alkene) and the different materials such as PS and PCL (polycaprolactone) composition, PLGA (poly lactide-glycolide acid).And fiber The preparation of shelf layer can be traditional injection molding, can also be emerging 3D printing method preparation.

Preferably, fibrous framework layer surface is handled through temperature sensing material grafting, temperature sensing material can be made to generate by changing temperature Phase transformation between hydrophilic and hydrophobic, so that the cell for being attached at the growth of fibrous framework layer be made to realize that Thermo-sensitive is de- after culture Fall harvest.The grafting processing of fibrous framework layer is handled in a conventional manner, which can lead to after culture It crosses and temperature is reduced to its phase transition temperature hereinafter, realizing the purpose that cell is detached from bracket automatically.In view of fitting for cell culture Suitable temperature is 37 DEG C or so, and the phase transformation critical-temperature of temperature sensing material is preferably 32 DEG C, then cell adherent growth temperature is 37 DEG C, real Best exfoliation temperature is 20 DEG C or so in testing.It should be understood that best exfoliation temperature in specifically used can be according to being cultivated The difference of cell type, and the difference of temperature sensing material is selected to set.

Certainly also optional, fibrous framework layer surface can also be handled without temperature sensing material grafting, not influence the device Cell culture effect need to be scraped using the trypsin digestion of chemistry or the cell scraper of physics in cell harvest Mode collects cell, and damage chemically or physically may be caused to cell.

Optionally, the number of plies of fibrous framework layer is 4-8 layers, and the present embodiment attached drawing is for 4 layers；And gap 303 is big It is small optional to be set as being 250-350 μm.The diameter of fiber filament 302 is chosen as 150-500 μm.In the present embodiment, common single The diameter of root fiber filament 302 is 400 μm, and there are 300 μ in the interweave in length and breadth gap 303 of fibrous framework layer of formation of plurality of fibers silk 302 M, 400 μm, 500 μm of three specifications.

Culture solution imports and exports mechanism, goes out including be suitable for being connected to by the closed plate of outer case 101 with toroidal cavity 104 Liquid component, and the feed liquor component suitable for being connected to by case lid 200 with hollow cavity 103.

Specifically, liquid component includes being adapted for insertion into toroidal cavity 104 and the outlet tube of nearly 101 bottom end of outer case out 401, what is be connected with outlet tube 401 goes out liquid peristaltic pump 402, and be connected by going out liquid connecting tube 403 with liquid peristaltic pump 402 out Eluate collection case 405.Liquid peristaltic pump 402 and eluate collection case 405 are optional directly solid with the closed plate at the top of outer case 101 out Even with realize the closed plate of outer case 101 for going out the support of liquid peristaltic pump 402 and eluate collection case 405, it is also optional, outside In addition setting goes out the support knot of liquid peristaltic pump 402 and eluate collection case 405 for support in the closed plate of the bottom of cabinet 101 Structure, in this regard, the present embodiment does not do absolute limit.

Since cell is in continuous growth course, corresponding metabolin can be generated, and the presence of metabolin will cause training The variation of the concentration of nutrient solution, so, the mode of addition culture solution can be taken to adjust culture to reach before replacing culture solution The purpose of the concentration of the culture solution of cell growth in cabinet 100, so, the feed liquor component that the present embodiment uses includes crossbearer At least two throwing liquid pipes 501 between 200 two sidewalls of case lid；The both ends of two throwing liquid pipes 501 are respectively connected with to distinguish shape At feed liquor first end 502 and feed liquor second end 503；Feed liquor first end 502 is that the hollow knot of 501 inner cavity of liquid pipe perforation is thrown with two Structure；Feed liquor second end 503 is that the solid construction of 501 inner cavity of liquid pipe isolation is thrown with two；Feed liquor first end 502 runs through case lid 200 Side wall to be connected with the feed liquor peristaltic pump 505 outside case lid 200；Feed liquor peristaltic pump 505 passes through feed liquor connecting tube and feed liquor cabinet 506 connections；And permeability pipeline made of the permselective membrane that liquid pipe 501 is porous structure is thrown, permeability pipeline is convenient for Culture solution is launched.It is equipped with the bayonet 201 for being suitable for overlapping feed liquor second end 503 in the side wall of case lid 200, and in case lid 200 side wall is additionally provided with the wears groove 203 passed through suitable for feed liquor first end 502, in order to enable feed liquor first end 502 and feed liquor second It is connected firmly between end 503 and the side wall of case lid 200, under the action of no external force applies, feed liquor first end 502 and feed liquor the It will not generate and separate between two ends 503 and case lid 200, be distributed with a washer in bayonet 201 and wears groove 203 and (do not marked in figure Note), washer is made of elastic material, such as silica gel material.It is optional for feed liquor peristaltic pump 505 and feed liquor cabinet 506 direct It is connected with the side wall of case lid 200, can also be realized by the supporting plate that the side wall in case lid 200 is arranged for feed liquor peristaltic pump 505 and feed liquor cabinet 506 support, in this regard, the present embodiment does not do absolute limit.

It please refers to shown in Fig. 2, optional, feed liquor component includes three throwing liquid pipes 501, and three are thrown the every adjacent of liquid pipe 501 Two throwing liquid pipes 501 between have a spacing, three throwing liquid pipes 501 are distributed with uniformly corresponding to internal box 102, so that logical It, can be to avoid the distribution of culture medium being newly added during crossing the addition that three throwing liquid pipes 501 carry out culture solution to internal box 102 Excessively the problems in collection allows new culture solution to uniformly being launched inside internal box 102, to provide cell growth Balanced environment.

It should be noted that feed liquor second end 503 completely cuts off between liquid pipe 501 due to throwing, in feed liquor second end 503 Do not have the circulation of culture solution inside, so, the main effect of the feed liquor second end 503 of the present embodiment design is and case lid 200 Side wall overlap joint.And for feed liquor first end 502 then throw perforation between liquid pipe 501 and can circulate culture solution, therefore for into Liquid first end 502 is not only used for being overlapped with the side wall of case lid 200 to cooperate feed liquor second end 503 to be collectively formed and throw liquid pipe 501 It is relatively fixed between case lid 200, also have and makes culture solution enter the work thrown in liquid pipe 501 by feed liquor first end 502 With.For the ease of throwing liquid pipe 501 is cleaned and sterilized after a period of use, liquid pipe 501 is thrown using elastic material Matter is made, such as elastic metallic material, the structure being designed in this way, and allows feed liquor first end 502 and into two ends from case lid 200 Side wall dismantled, herein it should be noted that feed liquor first end 502 is pierced by outside case lid 200 from the inside of case lid 200 The end in portion is removably connected with feed liquor peristaltic pump 505, in this way, to need to dismantle throwing liquid pipe 501 relative to case lid 200 When, feed liquor first end 502 can be separated from the side wall of case lid 200.

The main effect of the filtering tissue of the present embodiment setting is, needs to carry out the culture solution in culture cabinet 100 When replacement, so that the culture solution in the hollow cavity 103 of internal box 102 enters outer case 101 and internal box by filtering tissue The toroidal cavity 104 formed between 102 finally eventually enters into eluate collection case after going out liquid peristaltic pump 402 by outlet tube 401 405.In the process, by filtering tissue, so that the cell in the hollow cavity 103 of internal box 102 will not pass through filtering group It knits, that is, cannot achieve the circulation to toroidal cavity 104.

Due to the size of different cells be it is discrepant, for filtering tissue herein, difficulty or ease realize it is same The different size of cell of pseudometaplasia is filtered, so, if being not easy to replacement filtering tissue, it will be greatly reduced the regular cell mentioned The scope of application of culture apparatus substantially reduces its use value for medical experiment.Based on the above issues, the present embodiment The filtering tissue of design include the double medium filtration film 601 being arranged inside pillar hollow cylinder 603 and with pillar hollow cylinder 603 The hollow connector 602 being integrally connected.Hollow connector 602 is hollow structure, in order to culture solution circulation.Double medium filtration film 601 filter opening aperture is sequentially reduced by the direction of internal box 102 towards outer case 101.The size of the filter opening of double medium filtration film 601 Different specifications is set according to the cell of different sizes.The present embodiment design hollow connector 602 be mainly used for realize with Detachable connected between the side wall of internal box 102, the outside of specific hollow connector 602 is equipped with external screw thread, Yi Ji The hole wall of the aperture 105 of internal box 102 is equipped with the internal screw thread for being suitable for being adapted to the external screw thread of hollow connector 602, in this way, in needle When culture different size of cell, corresponding filtering tissue can be replaced.It needs to be illustrated herein, it is right In the size of cell, although the size of different cells there must be absolute difference, also need not for filtering tissue It to be directed to the design of different size of being customized of cell one by one, but inhomogeneity can be carried out according to different size of cell Other difference, i.e., it is corresponding using a kind of filtering tissue in a kind of cell of a certain size range, i.e., the size of cell is carried out one The division of range is determined, thus corresponding corresponding filtering tissue.

To in this present embodiment for the cabinet 102 that is included with the aperture 105 for filtering the internal box 102 that tissue assembles At least a pair of of aperture 105 that side wall opens up；And a pair of of aperture 105 is all set on the side wall of internal box 102 nearly internal box 102 Bottom position.A pair of of aperture 105 can be symmetrical arranged, and improve culture solution in internal box 102 in this way to toroidal cavity 104 The bottom position of nearly internal box 102 on the side wall of internal box 102 is arranged in the efficiency of circulation, and a pair of of aperture 105, so that During liquid peristaltic pump 402 extracts the culture solution in toroidal cavity 104 through outlet tube 401 out, be conducive to extracting Under the action of active force, accelerate velocity of liquid assets of the culture solution of internal box 102 to toroidal cavity 104.

It circulates for the ease of the gas outside the gas and incubator in internal box 102, is opened at the center of case lid 200 Equipped with a gas vent 701；Ventilative bacteriological filtration film 702 is covered at gas vent 701.Ventilative bacteriological filtration film 702 can pass through in order to gas, But it can passing through to avoid bacterium.Working as case lid 200 at the center of case lid 200 herein is circular configuration, then corresponding at center The center point, can also be other shapes of case lid 200 certainly.And it is optional for ventilative bacteriological filtration film 702 herein pass through strength Viscose glue connects with the edge of gas vent 701.In order to enable the cell in culture cabinet 100 can make full use of gas vent 701 Have the function that adjust the gas componant in culture cabinet 100, the surface area of gas vent 701 accounts for the upper end of whole case lid 200 The 1/3~1/2 of the surface area in face.

It please refers to shown in Fig. 1 and Fig. 3, in order to enable internal box 102 forms the favorable temperature environment for being suitable for cell growth, The bottom of internal box 102 is additionally provided with a heating plate 800 for being suitable for heating internal box 102；Internal box 102 uses heat conduction material Material is made.The culture solution in internal box 102 is heated by the heating plate 800 of internal box 102.Certainly here for having The temperature of culture solution in effect control internal box 102, it is also settable for sensing the temperature of culture-liquid temp in internal box 102 Sensor is connected with radio node adapter on temperature sensor, and is realized by the radio node adapter and controlled with external Wireless connection between the radio network gateway of device；And corresponding peripheral control unit includes radio receiving transmitting module, in this regard, the present embodiment It repeats no more.

It please refers to shown in Fig. 3, in order to enable culturing rack 300 is not directly attached to the bottom of the hollow cavity 103 of internal box 102 Portion farthest utilizes the surface area of culturing rack 300, is equipped with more pillars in the bottom of the hollow cavity 103 of internal box 102 110；More pillars 110 are equal relative to the height of 103 bottom of hollow cavity；And culturing rack 300 is supported in more pillars 110 On.In this way, not only making the bottom face formation of culturing rack 300 " insulting relative to the bottom of the hollow cavity 103 of internal box 102 It is empty " structure, and when bottom of the heating plate 800 for internal box 102 is heated, so that culturing rack 300 will not be by The even normal growth for influencing cell of uneven heating is caused in the directly bottom of contact internal box 102, so that culturing rack 300 is whole It is placed in uniformly heated culture solution, so that different cell growing environments is more balanced.

Above specific embodiment has carried out further in detail the purpose of this utility model, technical scheme and beneficial effects It describes in detail bright, it should be understood that the above are only specific embodiments of the present invention, it is practical new to be not limited to this Type, within the spirit and principle of the utility model, any modification, equivalent substitution, improvement and etc. done should be included in this Within the protection scope of utility model.

In the description of the present invention, it should be understood that indicating position or the term of positional relationship are based on attached drawing Shown in orientation or positional relationship, be merely for convenience of describing the present invention and simplifying the description, rather than indication or suggestion institute The equipment or element of finger must have a particular orientation, is constructed and operated in a specific orientation, therefore should not be understood as to this reality With novel limitation.

In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " Gu It is fixed " etc. terms shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral；It can be Mechanical connection, is also possible to be electrically connected；It can be directly connected, two can also be can be indirectly connected through an intermediary The interaction relationship of connection or two elements inside element.It for the ordinary skill in the art, can basis Concrete condition understands the concrete meaning of above-mentioned term in the present invention.

It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" be based on the orientation or positional relationship shown in the drawings, or The utility model product using when the orientation or positional relationship usually put, be merely for convenience of description the utility model and letter Change description, rather than the mechanism of indication or suggestion meaning or element must have a particular orientation, with specific orientation construct and Operation, therefore should not be understood as limiting the present invention.In addition, term " first ", " second ", " third " etc. are only used for area Divide description, is not understood to indicate or imply relative importance.

In addition, the terms such as term "horizontal", "vertical", " pendency " are not offered as requiring component abswolute level or pendency, and It is that can be slightly tilted.It is not to indicate the structure if "horizontal" only refers to that its direction is more horizontal with respect to for "vertical" It has to fully horizontally, but can be slightly tilted.

In the present invention unless specifically defined or limited otherwise, fisrt feature is on or below second feature It may include that the first and second features directly contact, also may include that the first and second features are not direct contacts but pass through it Between other characterisation contact.Moreover, fisrt feature is on second feature, top and above include fisrt feature the Right above two features and oblique upper, or first feature horizontal height is merely representative of higher than second feature.Fisrt feature is in the second spy Under sign, lower section and fisrt feature included below be directly below and diagonally below the second feature, or be merely representative of fisrt feature level Height is less than second feature.

Claims (10)

1. a kind of medical experiment cell culture apparatus characterized by comprising

Cabinet is cultivated, the culture cabinet includes internal box and is set to the outer container of internal box peripheral side around the internal box Body；The internal box has a hollow cavity and is set as corresponding to opening for the internal box bottom at the top of hollow cavity Mouthful；It is formed between the outer case and internal box at the top of toroidal cavity and the outer case and is set as closed plate；Described The side wall of internal box is equipped with the aperture for being suitable for being connected to the internal box and outer case；The tapping is provided with filtering tissue；

Case lid, the case lid are suitable for being covered on the opening of the internal box；

Culturing rack, the culturing rack are suitable for being placed in the hollow cavity of the culture cabinet for carrying cell；The culture Frame includes the fibrous framework layer of multiple stackings；And

Culture solution imports and exports mechanism, goes out liquid including be suitable for being connected to by the closed plate of the outer case with the toroidal cavity Component, and the feed liquor component suitable for being connected to by case lid with the hollow cavity.

2. medical experiment cell culture apparatus according to claim 1, which is characterized in that the liquid component out includes suitable In being inserted into the toroidal cavity and the outlet tube of nearly outer case bottom end, the liquid peristaltic pump out that is connected with the outlet tube, with And by going out liquid connecting tube and the eluate collection case that liquid peristaltic pump is connected out.

3. according to claim 1 or 2 described in any item medical experiment cell culture apparatus, which is characterized in that the feed liquor Component includes at least two piece throwing liquid pipes of the crossbearer between the case lid two sidewalls；

Two both ends for throwing liquid pipe are respectively connected with to be respectively formed feed liquor first end and feed liquor second end；The feed liquor One end is and two hollow structures for throwing the perforation of liquid pipe inner cavity；The feed liquor second end be and two throwing liquid pipe inner cavities The solid construction of isolation；

The feed liquor first end runs through the side wall of the case lid to be connected with the feed liquor peristaltic pump outside case lid；The feed liquor peristaltic pump It is connect by feed liquor connecting tube with feed liquor cabinet；And

It is described to throw permeability pipeline made of the permselective membrane that liquid pipe is porous structure.

4. medical experiment cell culture apparatus according to claim 1, which is characterized in that

The fibrous framework layer has a plurality of fiber filament, and a plurality of fiber filament is arranged in parallel；And

The fiber filament of fibrous framework layer described in every adjacent two layers is staggered；

The gap for cell attachment growth and culture solution circulation is formed between adjacent two fiber filaments being located on the same floor.

5. medical experiment cell culture apparatus according to claim 4, which is characterized in that the layer of the fibrous framework layer Number is 4-8 layers；And

The size in the gap is 250-350 μm.

6. medical experiment cell culture apparatus according to claim 1, which is characterized in that the filtering tissue includes it The pillar hollow cylinder of the double medium filtration film of interior setting and the hollow connector being integrally connected with the pillar hollow cylinder；

The outside of the hollow connector be equipped with external screw thread, and the internal box aperture hole wall be equipped be suitable for it is hollow The internal screw thread of the external screw thread adaptation of connector；

The filter opening aperture of the double medium filtration film is sequentially reduced by the direction of internal box towards outer case.

7. according to claim 1 or 6 described in any item medical experiment cell culture apparatus, which is characterized in that the interior case The aperture of body includes at least a pair of of aperture for opening up of side wall in the internal box；And

A pair of aperture is all set in the bottom position of nearly internal box on the side wall of the internal box.

8. medical experiment cell culture apparatus according to claim 1, which is characterized in that opened at the center of the case lid Equipped with a gas vent；

Ventilative bacteriological filtration film is covered at the gas vent.

9. medical experiment cell culture apparatus according to claim 1, which is characterized in that the bottom of the internal box is also The heating plate for being suitable for heating internal box equipped with one；The internal box is made of Heat Conduction Material.

10. medical experiment cell culture apparatus according to claim 1, which is characterized in that the internal box it is hollow The bottom of cavity is equipped with more pillars；The more pillars are equal relative to the height of the hollow cavity bottom；And

The culturing rack is supported on the more pillars.