CN208869584U - A kind of device being crushed DNA suitable for high-flux sequence - Google Patents
A kind of device being crushed DNA suitable for high-flux sequence Download PDFInfo
- Publication number
- CN208869584U CN208869584U CN201821476061.5U CN201821476061U CN208869584U CN 208869584 U CN208869584 U CN 208869584U CN 201821476061 U CN201821476061 U CN 201821476061U CN 208869584 U CN208869584 U CN 208869584U
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- Prior art keywords
- cabinet
- broken
- fixedly installed
- flux sequence
- crushed
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000523 sample Substances 0.000 claims abstract description 20
- 238000013016 damping Methods 0.000 claims abstract description 7
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 12
- 239000011435 rock Substances 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 3
- 108020004414 DNA Proteins 0.000 description 21
- 238000012163 sequencing technique Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000012488 sample solution Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 3
- 238000013467 fragmentation Methods 0.000 description 3
- 238000006062 fragmentation reaction Methods 0.000 description 3
- 238000002525 ultrasonication Methods 0.000 description 3
- 238000002604 ultrasonography Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of suitable for high-flux sequence is crushed the device of DNA, including cabinet, ultrasonic transducer is fixedly installed at the top of the cabinet, probe component is fixedly installed at the top of the inner wall of the cabinet, the cabinet wall bottom is fixedly installed with mounting base, broken box is plugged at notch above the mounting base, the bottom of the cabinet is provided with damping part, the left and right ends interior of the cabinet is provided with noise reduction layer, a kind of device being crushed DNA suitable for high-flux sequence of the utility model, not only simple but also practical, it can be equivalent, it is convenient, fast, low investment, it is broken to carry out to low consumption DNA.
Description
Technical field
The utility model relates to a kind of DNA crushing device, specifically a kind of dress that DNA is crushed suitable for high-flux sequence
It sets.
Background technique
High throughput sequencing technologies are also known as " next generation " sequencing technologies, with can be once parallel to hundreds of thousands to millions of DNA
Molecule progress sequencing and generally reading length are shorter to be waited to indicate, in sequencing technologies, the sequencing library for constructing high quality is height
The key factor of flux sequencing, the quality of sequencing library directly determines the quality of data of sequencing output, and then has to data analysis
Greatest association, sequencing library building beginning, first will to DNA sample carry out fragmentation, ultrasonic wave break method is more
The features such as method of ideal DNA fragmentation, has fragmentation accurate, and success rate is high.
The device of most of ultrasonication DNA is expensive, experimentation is complicated, experiment flux is relatively low, experiment is set
Standby maintenance replacement cost is high, it is difficult to meet the needs of public laboratory, it is therefore desirable to the new easily operated broken DNA's of one kind
Method, can either precisely, high efficiency, high-throughput broken segment, consumptive material, instrument cost can be substantially reduced again.
Utility model content
The purpose of this utility model is to provide a kind of suitable for high-flux sequence is crushed the device of DNA, on solving
State the problem of proposing in background technique.
To achieve the above object, the utility model provides the following technical solutions:
A kind of device being crushed DNA suitable for high-flux sequence, including cabinet are fixedly installed at the top of the cabinet
The inner wall top of ultrasonic transducer, the cabinet is fixedly installed with probe component, and the cabinet wall bottom is fixedly installed with
Mounting base is plugged with broken box at the notch above the mounting base, and the bottom of the cabinet is provided with damping part, the case
The left and right ends interior of body is provided with noise reduction layer.
Further scheme as the utility model: the probe component includes electric pushrod, and the electric pushrod is fixed
It is mounted at the top of cabinet wall, the bottom end of the electric pushrod is fixedly mounted in mounting disc, on the lower end surface of the mounting disc
Several equally distributed ultrasonic probes are fixedly installed with, the ultrasonic probe is electrically connected with ultrasonic transducer.
Further scheme as the utility model: the broken box includes PCR plate, if offering in the PCR plate
Dry equally distributed broken hole and label plate, the label plate are solid on the front side in broken hole, the lower end surface of the PCR plate
Dingan County is equipped with several equally distributed broken pipes, and the broken pipe is corresponding with the position in broken hole.
Further scheme as the utility model: it is equally distributed that several are provided on the side wall of the broken pipe
Protrusion is provided with graduation mark on the lateral wall of the broken pipe, is provided with slot on the rocks between the broken pipe.
Further scheme as the utility model: being provided with dustproof cover on the broken box, the dustproof cover is located at
It is crushed the top of box, offers sliding groove on the left and right sides end face of the PCR plate, the sliding groove is internally provided with sliding
Block, the sliding shoe are fixedly installed with connecting plate on face, and the upper surface of the connecting plate and the lower end surface of dustproof cover are solid
Fixed connection.
Further scheme as the utility model: the damping part includes pedestal, the upper surface of the pedestal with
It is fixedly connected between the lower end surface of cabinet by several equally distributed telescopic rods, movable sleeve is connected to bullet on the telescopic rod
Spring, the both ends of the spring are fixedly connected with the lower end surface of the upper surface of pedestal and cabinet respectively.
Compared with prior art, a kind of device being crushed DNA suitable for high-flux sequence of the utility model, is using
When, solution is instilled in the broken pipe in broken box, is accurately required since the component of solution has, graduation mark is convenient to solution
Dosage is observed, while experiment generally records the number of sample, label plate be can be used to carry out label, is being added dropwise
When sample solution, since broken pipe quantity is more, the time is longer, to prevent solution contaminated, is existed using dustproof cover by sliding shoe
Sliding groove internal slide covers the broken hole for having added solution, avoids sample solution contaminated, broken box is placed on mounting base
On, dustproof cover is removed, since a large amount of heat can be generated during ultrasonication, so that solution temperature increases, therefore on the rocks
Ice cube is added in slot, for adjusting the temperature of solution, after energization, electric pushrod operating drives mounting disc to move downward, and ultrasound is visited
Head is inserted into the solution in broken pipe, and ultrasonic transducer operating, ultrasonic probe transmits ultrasound to sample solution, causes a large amount of gas
Bubble, bubble bursts broken DNA, in shattering process, generates noise, is absorbed by the acoustical panel of noise reduction layer, the shake of the cabinet of initiation
It is dynamic, alleviated by telescopic rod and spring；A kind of device being crushed DNA suitable for high-flux sequence of the utility model,
Not only simple but also practical, can equivalent, convenient, fast, low investment, to carry out to low consumption DNA broken.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of device that DNA is crushed suitable for high-flux sequence；
The overlooking structure figure of box is crushed in the device that Fig. 2 is a kind of DNA broken suitable for high-flux sequence；
The left view structure chart of box is crushed in the device that Fig. 3 is a kind of DNA broken suitable for high-flux sequence；
Fig. 4 is the structural schematic diagram of dustproof cover in the device of DNA broken suitable for high-flux sequence a kind of.
In figure: 1, cabinet；2, ultrasonic transducer；3, electric pushrod；4, mounting disc；5, ultrasonic probe；6, noise reduction layer；7,
Mounting base；8, it is crushed box；9, pedestal；10, telescopic rod；11, spring；12, PCR plate；13, it is crushed hole；14, it is crushed pipe；15, convex
It rises；16, graduation mark；17, slot on the rocks；18, label plate；19, sliding groove；20, dustproof cover；21, connecting plate；22, sliding shoe.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
Fig. 1-4, a kind of device being crushed DNA suitable for high-flux sequence, including cabinet 1 are please referred to, the cabinet 1
Top is fixedly installed with ultrasonic transducer 2, is fixedly installed with probe component at the top of the inner wall of the cabinet 1, in the cabinet 1
Wall bottom is fixedly installed with mounting base 7, is plugged with broken box 8, the bottom of the cabinet 1 at the notch of 7 top of mounting base
It is provided with damping part, the left and right ends interior of the cabinet 1 is provided with noise reduction layer 6.
The probe component includes electric pushrod 3, and the electric pushrod 3 is fixedly mounted at the top of 1 inner wall of cabinet, the electricity
The bottom end of dynamic push rod 3 is fixedly mounted in mounting disc 4, several are fixedly installed on the lower end surface of the mounting disc 4 and is uniformly divided
The ultrasonic probe 5 of cloth, the ultrasonic probe 5 are electrically connected with ultrasonic transducer 2.
The broken box 8 includes PCR plate 12, offers several equally distributed broken 13 Hes of hole in the PCR plate 12
Label plate 18, the label plate 18 are located at the front side in broken hole 13, are fixedly installed with several on the lower end surface of the PCR plate 12
Equally distributed broken pipe 14, the broken pipe 14 are corresponding with the position in broken hole 13.
Be provided on the side wall of the broken pipe 14 several equally distributed raised 15, the lateral wall of the broken pipe 14
On be provided with graduation mark 16, be provided with slot 17 on the rocks between the broken pipe 14.
Dustproof cover 20 is provided on the broken box 8, the dustproof cover 20 is located at the top of broken box 8, the PCR plate 12
Left and right sides end face on offer sliding groove 19, the sliding groove 19 is internally provided with sliding shoe 22, the sliding shoe 22
Be fixedly installed with connecting plate 21 on the face, the upper surface of the connecting plate 21 is fixedly connected with the lower end surface of dustproof cover 20.
The damping part includes pedestal 9, passes through several between the upper surface of the pedestal 9 and the lower end surface of cabinet 1
Equally distributed telescopic rod 10 is fixedly connected, and movable sleeve is connected to spring 11, the both ends point of the spring 11 on the telescopic rod 10
It is not fixedly connected with the lower end surface of the upper surface of pedestal 9 and cabinet 1.
The working principle of the utility model is: a kind of device that DNA is crushed suitable for high-flux sequence, when in use,
Solution is instilled in the broken pipe 14 in broken box 8, is accurately required since the component of solution has, graduation mark 16 is convenient to solution
Dosage observed, while experiment generally the number of sample is recorded, can be used label plate 18 progress label,
When sample solution is added dropwise, since broken 14 quantity of pipe is more, the time is longer, to prevent solution contaminated, is passed through using dustproof cover 20
Sliding shoe 22 covers the broken hole 13 for having added solution in 19 internal slide of sliding groove, avoids sample solution contaminated, will be crushed
Box 8 is placed in mounting base 7, removes dustproof cover 20, due to that can generate a large amount of heat during ultrasonication, so that solution
Temperature increases, therefore ice cube is added in slot 17 on the rocks, and for adjusting the temperature of solution, after energization, electric pushrod 3 is operated, band
Dynamic mounting disc 4 moves downward, and ultrasonic probe 5 is inserted into the solution in broken pipe 14, and ultrasonic transducer 2 operates, ultrasonic probe 5
Ultrasound is transmitted to sample solution, causes a large amount of bubbles, bubble bursts broken DNA, in shattering process, noise is generated, by noise reduction
The acoustical panel of layer 6 absorbs, and the vibration of the cabinet 1 of initiation is alleviated by telescopic rod 10 and spring 11.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, on being understood by concrete condition
State the concrete meaning of term in the present invention.
The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party
Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective
Various changes can be made.
Claims (6)
1. a kind of device for being crushed DNA suitable for high-flux sequence, including cabinet (1), which is characterized in that the cabinet (1)
Top be fixedly installed with ultrasonic transducer (2), be fixedly installed with probe component at the top of the inner wall of the cabinet (1), it is described
Cabinet (1) inner wall bottom is fixedly installed with mounting base (7), is plugged with broken box (8) at the notch above the mounting base (7),
The bottom of the cabinet (1) is provided with damping part, and the left and right ends interior of the cabinet (1) is provided with noise reduction layer
(6)。
2. a kind of device for being crushed DNA suitable for high-flux sequence according to claim 1, which is characterized in that described
Probe component includes electric pushrod (3), and the electric pushrod (3) is fixedly mounted at the top of cabinet (1) inner wall, the electric pushrod
(3) bottom end is fixedly mounted on mounting disc (4), several are fixedly installed on the lower end surface of the mounting disc (4) and is uniformly divided
The ultrasonic probe (5) of cloth, the ultrasonic probe (5) are electrically connected with ultrasonic transducer (2).
3. a kind of device for being crushed DNA suitable for high-flux sequence according to claim 1, which is characterized in that described
Broken box (8) include PCR plate (12), several equally distributed broken holes (13) and label are offered on the PCR plate (12)
Plate (18), the label plate (18) are located at the front side of broken hole (13), the PCR plate (12) if lower end surface on be fixedly installed with
Dry equally distributed broken pipe (14), the broken pipe (14) are corresponding with the position of broken hole (13).
4. a kind of device for being crushed DNA suitable for high-flux sequence according to claim 3, which is characterized in that described
It is provided on the side wall of broken pipe (14) several equally distributed raised (15), is arranged on the lateral wall of the broken pipe (14)
Have graduation mark (16), is provided with slot on the rocks (17) between the broken pipe (14).
5. a kind of device for being crushed DNA suitable for high-flux sequence according to claim 3, which is characterized in that described
It is provided with dustproof cover (20) on broken box (8), the dustproof cover (20) is located at the top of broken box (8), the PCR plate (12)
It is offered on the end face of the left and right sides sliding groove (19), the sliding groove (19) is internally provided with sliding shoe (22), the sliding
Block (22) is fixedly installed with connecting plate (21) on face, the upper surface of the connecting plate (21) and the lower end of dustproof cover (20)
Face is fixedly connected.
6. a kind of device for being crushed DNA suitable for high-flux sequence according to claim 1, which is characterized in that described
Damping part includes pedestal (9), is uniformly divided between the upper surface of the pedestal (9) and the lower end surface of cabinet (1) by several
The telescopic rod (10) of cloth is fixedly connected, and movable sleeve is connected to spring (11) on the telescopic rod (10), the both ends of the spring (11)
It is fixedly connected respectively with the lower end surface of the upper surface of pedestal (9) and cabinet (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821476061.5U CN208869584U (en) | 2018-09-11 | 2018-09-11 | A kind of device being crushed DNA suitable for high-flux sequence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821476061.5U CN208869584U (en) | 2018-09-11 | 2018-09-11 | A kind of device being crushed DNA suitable for high-flux sequence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208869584U true CN208869584U (en) | 2019-05-17 |
Family
ID=66467208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821476061.5U Expired - Fee Related CN208869584U (en) | 2018-09-11 | 2018-09-11 | A kind of device being crushed DNA suitable for high-flux sequence |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208869584U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113214988A (en) * | 2021-05-31 | 2021-08-06 | 南京溯远基因科技有限公司 | Device suitable for broken DNA in high-throughput sequencing |
-
2018
- 2018-09-11 CN CN201821476061.5U patent/CN208869584U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113214988A (en) * | 2021-05-31 | 2021-08-06 | 南京溯远基因科技有限公司 | Device suitable for broken DNA in high-throughput sequencing |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190517 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |