CN208834754U - One kind is based on just setting microscopical single beam laser light forceps device - Google Patents
One kind is based on just setting microscopical single beam laser light forceps device Download PDFInfo
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- CN208834754U CN208834754U CN201821524891.0U CN201821524891U CN208834754U CN 208834754 U CN208834754 U CN 208834754U CN 201821524891 U CN201821524891 U CN 201821524891U CN 208834754 U CN208834754 U CN 208834754U
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Abstract
One kind is based on just setting microscopical single beam laser light forceps device, including laser, optical isolator, reflecting mirror, dichroscope, light beam hoisting frame, microcobjective, motorized precision translation stage, Charge Coupled Device.The present invention is based on just set microscopical light forceps device, the demand that Lai Shixian laser optical tweezer places the prone position that the single red blood cell of the living body cerebrovascular captures.
Description
Technical field
The present invention relates to the laser optical tweezer that a kind of single beam laser of continuous wave laser transmitting is formed in the case where just setting microscope, tools
Body is that one kind is based on just setting microscopical single beam laser light forceps device.
Background technique
Optical tweezer is the new tool for manipulating micro-nano magnitude particle and measuring skin ox magnitude power.Optical tweezer, i.e. optical tweezers, have
The ability of optical manipulation and weak interaction measurement.Optical tweezer captures small grain by light and object interaction, in three-dimensional space
Son has the characteristics that no Mechanical Contact, remote-controlled operation, does not interfere ambient enviroment locating for particle.In liquid environment, optical tweezer
It can suspend, fix, arranging, manipulating, constructing, sorting and rotating particle.In addition, optical tweezer applies active force to object by light,
Active force is less than 200pN.Therefore, optical tweezer is also the probe of small power, can be used for the weak interaction measurement of microscopic fields.
Laser optical tweezer is in vitro and vital red cell operation research.Laser optical tweezer is passed through using single beam laser has high number
The microcobjective for being worth aperture carries out strong-focusing and generates three-dimensional ligh trap.Optical tweezer can be big to single biology close under physiological status
Molecule, organelle or cell are identified, the vital movement of biological cell is tracked；It is especially red to single red blood cell or lesion thin
Born of the same parents identify or the emergency reaction of the single red blood cell of long-time real-time monitoring.In mobiles, existing optical tweezer technology is
It is applied in living body ear capillary in the research of red blood cell.
The limitation that laser optical tweezer captures living body cerebrovascular red blood cell.Laser optical tweezer currently used for viviperception is main
It is designed using inverted microscope mode, since this mode is conducive to the observation of biological sample, and is set with other auxiliary
Standby united application.However, laboratory sample in viviperception, is generally placed on by the optical optical tweezers system under the mode with dorsal position
The top of microcobjective, ear's capillary that this mode causes the system to be confined to biopsy sample are studied.And working as forebrain
In the cerebrovascular research of scientific domain, the optical optical tweezers system based on inverted microscope mode is difficult to realize correlation function.Compared to
Microscopy mode is set, the light forceps device for just setting microscopy mode may be implemented to be the living body cerebrovascular, spinovascular research
Living body biological sample can be placed under microcobjective by this mode with prone position, this is that inverted microscope mode cannot achieve
's.
The limitation that light forceps device is applied in brain science field proposes new challenge to the development of optical tweezer technology.
Summary of the invention
The object of the present invention is to provide one kind based on microscopical single beam laser light forceps device is just set, to solve above-mentioned ask
At least one aspect of topic.
According to an aspect of the present invention, it provides one kind to be based on just setting microscopical single beam laser light forceps device, including swashs
Light device, optical isolator, reflecting mirror, dichroscope, light beam hoisting frame, microcobjective, motorized precision translation stage, Charge Coupled Device；
The laser is for emitting single beam laser；
The optical isolator protects laser for reflected laser to be isolated；Face the emission port of laser
Setting；
The reflecting mirror for changing laser the direction of propagation；
The light beam hoisting frame is oppositely arranged with optical isolator, for improving the level height of light beam；Reflecting mirror is placed in
On light beam hoisting frame；
The camera lens and dichroscope of the microcobjective are oppositely arranged at 45 degree, form optical tweezer for focused laser beam；
The dichroscope is penetrated according to wavelength or reflected light, and dichroscope will transmit through light and be introduced into the micro- object just set
In mirror, is focused in the solution of sample cell and form optical tweezer, and capture target sample；
The motorized precision translation stage is placed in below the camera lens of microcobjective, the movement for sample；
It further include illumination path, illumination path includes condenser, halogen lamp, and the scattering light that halogen lamp issues is poly- through condenser
Light provides illumination；
The Charge Coupled Device is used to detect the capture effect of optical tweezer, records catching position；Halogen lamp is saturating through condenser
It penetrates light and reflexes to Charge Coupled Device through dichroscope.Charge Coupled Device is connected with computer, in real-time monitoring sample cell
Sample.
The beneficial effects of the present invention are: Lai Shixian laser optical tweezer is to work the present invention is based on microscopical light forceps device is just set
The demand that the prone position of the single red blood cell capture of the body cerebrovascular is placed.
Detailed description of the invention
Fig. 1 is a kind of based on the implementation diagram for just setting microscopical optical optical tweezers system.
1- semiconductor laser, 2- optical isolator, 3- reflecting mirror, 4- reflecting mirror, 5- reflecting mirror, 6- dichroscope, 7-
Microcobjective, 8- moveable stage, 9- condenser, 10- halogen light source, 11- Charge Coupled Device, 12- computer.
Specific embodiment
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, with reference to the accompanying drawing, to this
Invention is further detailed.
Embodiment 1
The green laser that solid state laser exports that a branch of wavelength is 532nm first is introduced into after optical isolator shaping
It in the optical path hoisting frame being made of reflecting mirror, and is introduced into the microcobjective just set by dichroscope, in the molten of sample cell
It is focused in liquid and forms optical tweezer.The parameter of microcobjective: amplification factor 60, numerical aperture 1.0, water immersion objective.At this point, laser function
Rate is 15mW or less.The scattering light that halogen lamp issues provides illumination through condenser optically focused.Pass through the electronics connecting with microcobjective
Coupled apparatus and computer are used to observe the dynamic process in sample cell.
Above-mentioned laser is 532nm solid green laser, MGL-H-532nm type.Microcobjective is Olympus brand,
LUMPLFLN60XW(60×,N.A.1.0).Optical isolator is Daheng's photoelectricity brand；Reflecting mirror is GCC-101102 type broadband
Deielectric-coating high reflection mirror (450-700nm, Φ 25.4)；Dichroscope is Φ 12.7mm, transmitted infrared light, vitreous silica system；Electricity
Sub- coupled apparatus is MER-310-120UC type industrial camera CCD, HxV (656 × 492)；It is provided by Daheng's photoelectricity.Light beam mentions
Rising frame is the level height device that optics commonly improves light beam；Motorized precision translation stage is the common linear motor translation stage of optics.
Embodiment 2
Selecting diameter is 1 μm to 2 μm of polystyrene sphere.And bead is suspended in sample cell with distilled water.It is micro-
The hydroscope that object lens select numerical aperture to be higher than 1.0.Solid state laser exports the green laser that a branch of wavelength is 532nm, through optics
After isolator shaping, be introduced into the optical path hoisting frame being made of reflecting mirror, and be introduced by dichroscope just setting it is micro-
In object lens, is focused in the solution of sample cell and form optical tweezer, and capture polystyrene sphere.Illumination path is introduced as through condenser
The light field imaging of bead provides illumination, and transmitted light reflexes to Charge Coupled Device through dichroscope, remembered by Charge Coupled Device
Record small ball position.Charge Coupled Device is connected with computer, the polystyrene sphere in real-time monitoring sample cell.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (4)
1. one kind is based on just setting microscopical single beam laser light forceps device, it is characterised in that: including laser, be optically isolated
Device, reflecting mirror, dichroscope, light beam hoisting frame, microcobjective, motorized precision translation stage, Charge Coupled Device；
The laser is for emitting single beam laser；
The optical isolator protects laser for reflected laser to be isolated；Face the emission port setting of laser；
The reflecting mirror for changing laser the direction of propagation；
The light beam hoisting frame is oppositely arranged with optical isolator, for improving the level height of light beam；Reflecting mirror is placed in light beam
On hoisting frame；
The camera lens and dichroscope of the microcobjective are oppositely arranged at 45 degree, form optical tweezer for focused laser beam；
The dichroscope is penetrated according to wavelength or reflected light, and dichroscope will transmit through light and be introduced into the microcobjective just set
In, it is focused in the solution of sample cell and forms optical tweezer, and capture target sample；
The motorized precision translation stage is placed in below the camera lens of microcobjective, the movement for sample；
The Charge Coupled Device is oppositely arranged with dichroscope, for detecting the capture effect of optical tweezer, records catching position；Electricity
Sub- coupled apparatus is connected with computer, the sample in real-time monitoring sample cell.
2. one kind according to claim 1 is based on just setting microscopical single beam laser light forceps device, it is characterised in that: also
Including illumination path, illumination path includes condenser, halogen lamp, and the scattering light that halogen lamp issues provides photograph through condenser optically focused
It is bright.
3. one kind according to claim 2 is based on just setting microscopical single beam laser light forceps device, it is characterised in that: halogen
Plain lamp reflexes to Charge Coupled Device through dichroscope through condenser transmitted light.
4. one kind according to claim 1 is based on just setting microscopical single beam laser light forceps device, it is characterised in that: light
Three reflecting mirrors are arranged in beam hoisting frame, change the direction of propagation of laser.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821524891.0U CN208834754U (en) | 2018-09-18 | 2018-09-18 | One kind is based on just setting microscopical single beam laser light forceps device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821524891.0U CN208834754U (en) | 2018-09-18 | 2018-09-18 | One kind is based on just setting microscopical single beam laser light forceps device |
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| CN208834754U true CN208834754U (en) | 2019-05-07 |
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| CN201821524891.0U Expired - Fee Related CN208834754U (en) | 2018-09-18 | 2018-09-18 | One kind is based on just setting microscopical single beam laser light forceps device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109147983A (en) * | 2018-09-18 | 2019-01-04 | 湖北第二师范学院 | One kind is based on just setting microscopical single beam laser light forceps device |
| CN116013575A (en) * | 2022-12-21 | 2023-04-25 | 深圳大学 | Low-temperature optical heating tweezers control system and method based on water abnormal expansion characteristics |
-
2018
- 2018-09-18 CN CN201821524891.0U patent/CN208834754U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109147983A (en) * | 2018-09-18 | 2019-01-04 | 湖北第二师范学院 | One kind is based on just setting microscopical single beam laser light forceps device |
| CN116013575A (en) * | 2022-12-21 | 2023-04-25 | 深圳大学 | Low-temperature optical heating tweezers control system and method based on water abnormal expansion characteristics |
| CN116013575B (en) * | 2022-12-21 | 2023-08-22 | 深圳大学 | Low-temperature optical heating tweezers control system and method based on water abnormal expansion characteristics |
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| GR01 | Patent grant | ||
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190507 Termination date: 20190918 |