CN110993141A - Multi-core optical fiber suspension type optical motor system - Google Patents
Multi-core optical fiber suspension type optical motor system Download PDFInfo
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- CN110993141A CN110993141A CN201911120314.4A CN201911120314A CN110993141A CN 110993141 A CN110993141 A CN 110993141A CN 201911120314 A CN201911120314 A CN 201911120314A CN 110993141 A CN110993141 A CN 110993141A
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Abstract
The invention provides a multi-core optical fiber suspension type optical motor system with cell capturing and rotating functions. The optical motor system includes: a laser 1 for generating a desired light source; a single mode optical fiber 2; an optical fiber splitter 3; an attenuator 4; a multi-core optical fiber combiner 5; a multi-core optical fiber 6; a sample cell 7; a microscopic imaging system 8; an optical power meter 9. The invention can be used for the capture of particles, single cells or multiple cells and the rotation operation thereof. Can be widely used in the fields of cell analysis, drug development and micro-manufacturing.
Description
(I) technical field
The invention relates to a multi-core optical fiber suspension type optical motor system which can be used for capturing particles, single cells or multiple cells and rotating the particles, the single cells or the multiple cells. Can be widely used in the fields of cell analysis, drug development and micro-manufacturing.
(II) background of the invention
A multi-core optical fiber suspension type optical motor system is a development and application of optical tweezers.
In the field of optical tweezers, the Ashkin laboratory group in bell laboratories has made pioneering work. In 1970, Ashkin speculated that the focused laser could push Particles on the micron scale by estimation (Ashkin, A. Acceraction and tracking of Particles by Radiation Pressure [ J ]. Physical Review Letters,1970,24(4): 156-. He placed latex microspheres with a diameter of 0.6-2.5 um in water and focused a two virtual argon ion laser at a power of 1w in the water, and found that these particles can be accelerated along the optical axis. This experiment was the first to clearly observe the effects of light pressure and confirm his speculation.
In 1985, when studying single beam laser capture of atoms, Ashkin attempted to grab larger particles with a similar device and found that: these particles can be stably captured by only highly focusing a single laser beam. Thus, in 1986, Ashkin et al indicated that a single laser was highly focused and that particles could be stably captured at the laser beam focal point (occlusion of single-beam gradient optical trap for electronic particles). The optical trapping of such a single laser beam becomes "optical tweezers" which can grab particles with diameters ranging from tens of nanometers to tens of micrometers without mechanical contact.
The property of optical tweezers that can manipulate microparticles without mechanical contact has attracted considerable interest in the life sciences community. In 1987, Ashkin et al first used optical tweezers to capture bacterial viruses in life sciences. The optical tweezers technology is widely applied to life science research, and provides a powerful tool for cell research and biological pharmacy.
Cell biology is a subject for studying the structure, function and various life laws of cells at the microscopic, sub-microscopic and molecular levels by applying modern physical, chemical and molecular biological methods. It developed from cytology. Since cells have already been investigated not only purely for individual cells, organelles and biological macromolecules or for individual life phenomena, but also for organic integration of these, their interrelations and their relationship to the environment are explored from dynamic processes.
The experiment that polystyrene particles are suspended by single-mode optical fiber by using the principle of optical suspension is designed in the optical mechanical effect experiment (Zhang Yu, Hou Hua, Liu Shi, etc.. the optical mechanical effect experiment [ J ]. the physical experiment, 2010,30(10):8-10.DOI:10.3969/J. issn.1005-4642.2010.10.004.) so as to observe the light radiation pressure. Designs a relatively mature experimental device, has simple structure and easy operation. But the function is single, and the ball can only be grabbed and cannot be rotated.
The invention patent with the publication number of CN104765165B introduces a method and a device for rapid optical suspension of microspheres, which comprises a transparent sensing unit, wherein a cavity is arranged in the sensing unit and used for providing space for optical suspension of the microspheres, a first hole and a second hole are arranged on the surface layer of the sensing unit, the first hole is used for providing a passage for the microspheres to enter the cavity, the second hole is used for providing a passage for the microspheres to exit the cavity, and a fundamental mode Gaussian beam is focused on the microspheres. The sensing unit is connected with a heater, and the heater is used for heating the microsphere solution in the cavity of the sensing unit. The invention adopts space optics, compared with a method for transmitting laser by an optical fiber, the method has a complex structure, and the whole device is inflexible and is not easy to change the experimental place.
The invention patent with publication number CN108873294A discloses a device for capturing particles or cells by using dual-laser optical tweezers, which comprises a device body, wherein the device body comprises a microscope and an optical tweezers device arranged on the microscope, the microscope comprises a base, an object stage and a lens barrel from bottom to top, the bottom of the lens barrel is provided with an objective lens, the top of the lens barrel is provided with an eyepiece, a particle or cell chamber is arranged below the objective lens, the particle or cell chamber is arranged on the object stage, the optical tweezers device comprises a first optical tweezers emitter and a second optical tweezers emitter which are arranged on the base, and laser optical tweezers generated by the first optical tweezers emitter and the second optical tweezers emitter pass through light holes on the object stage and focus on two sides of the particles or cells after passing through the light holes on the object stage. Compared with the traditional optical tweezers technology, the two laser optical tweezers emitters provided by the invention have the advantages that the cell volume and the cell mass extracted by the emitters are 3-5 times of those of the ordinary optical tweezers technology, and the optical tweezers effect of driving, controlling or separating a plurality of particles is achieved. However, this method can capture only cells, and cannot realize complicated operations such as cell rotation.
Under the above background, the present invention provides a multi-core optical fiber suspension type optical motor system. The optical fiber core can capture cells, and can control the cells to rotate in multiple directions by adjusting the output optical field of each fiber core, so that the cell posture can be adjusted. Compared with the prior art, the invention adopts the novel multi-core optical fiber, highly integrates multiple paths of light beams, and has small volume and low manufacturing cost. The invention provides a powerful tool for cell tomography and has profound significance for cell analysis, biological research and micro-manufacturing.
Disclosure of the invention
The invention aims to provide a multi-core optical fiber suspension type optical motor system which can be used for cell capture, rotation and micro-manufacturing.
The purpose of the invention is realized as follows:
fig. 1 the optical motor system includes: a laser 1 for generating a desired light source; a single mode optical fiber 2; an optical fiber splitter 3; an attenuator 4; a multi-core optical fiber combiner 5; a multi-core optical fiber 6; a sample cell 7; a microscopic imaging system 8; an optical power meter 9. Laser in the system is emitted from a laser 1 and passes through a standard single-mode fiber 2 to a fiber light splitter 3, and one laser beam is divided into N +1 laser beams. One of the lasers is connected to an optical power meter 9 for measuring the divided laser power. The attenuator 4 comprises N attenuators each connected to a laser beam split from the fiber splitter for individually adjusting the laser power. N optical fibers led out from the attenuator are connected to the input end 6 of the multi-core optical fiber beam combiner, and the multi-core optical fiber beam combiner 5 is connected with the multi-core optical fiber 6. The state of the input laser light to each core in the multicore fiber can be individually controlled by the attenuator 4. The multicore fiber 6 is inserted into the bottom of the experimental sample cell 7, with the fiber perpendicular to the horizontal plane. The test sample cell 7 contains the particles to be manipulated. The microscopic imaging system 8 can observe experimental operating conditions in real time. One core of the multicore fiber 6 captures and "lifts" the cell to be manipulated against gravity using optical trapping forces. The power of the optical fields emitted by the different fiber cores is controlled by the attenuator 4, and the scattering force of the attenuator is utilized to enable the cells to have enough rotating torque, so that the particles rotate in different directions. The rotating cellular phase acts as a "rotor" and the laser provides the motive force, forming an optical motor system.
A multi-core optical fiber suspension type cell optical motor system can complete the operations of cell capture and rotation only by using a multi-core optical fiber, and the optical fiber penetrates from the bottom of a sample cell and is vertical to the horizontal plane.
The multi-core optical fiber used in the system is characterized in that: the optical fiber is provided with N fiber cores, wherein one fiber core is a middle core, and the other fiber cores are distributed around the middle core in a ring array manner. As shown in fig. 2, the pictures illustrate only five-core and seven-core optical fibers, but are not limited to these two.
The system uses two-dimensional light trap force formed by a light field emitted by an intermediate core, scattering force in the light field overcomes cell gravity, and gradient force binds cells to an optical axis. As shown in fig. 3. By adjusting the attenuator 4, the corresponding fiber core emits the optical field, and the cell can be rotated. The optical field of each core can be independently controlled by the attenuator 4.
The force of the optical field generated by each fiber core on the cell is as follows:
the force acting on a cell of volume V can be seen as a tension on the surface S of the cell,
is the tension tensor acting on a unit area. The force acting on the surface element is
Wherein
In the formula (I), the compound is shown in the specification,
Tijthe meaning of (1) is the component of the force acting on a unit area perpendicular to the j axis on the i axis. n isjIs the outward normal vector of the cell' S outer surface S perpendicular to the j-axis.
The attenuator array 5 can be adjusted to make the optical field of any fiber core reach the appropriate intensity. When the intermediate core generates a scattering force FsBuoyancy of time and cell FfGravity FgSatisfies the condition Fg＝FS+FfWhen the resultant force exerted on the cell in the longitudinal direction is zero, the cell is stably captured.
If the cell rotation needs to be controlled, the attenuator 4 is adjusted, and the optical field emitted by the corresponding rotation control core is controlled according to the requirements of different rotation angles, and the optical field can provide momentum for the side face of the cell. One attenuator for each core, so that the optical field of each core can be controlled independently. The relationship of the torque M acting on the cells is
Wherein the content of the first and second substances,
is a unit vector in the y direction in a rectangular coordinate system,
is a position vector with respect to the axis of rotation,
is the outward normal vector of the cell' S outer surface S.
As time average of surface tension
Compared with the prior art, the invention has the outstanding advantages that:
1. the structure is simple. The optical device only needs a laser, an optical fiber splitter, an attenuator and an optical fiber beam combiner, and the connection mode does not need precise mechanical connection.
2. The functions are rich and the integration is high. Because one multicore fiber comprises a plurality of fiber cores and the optical field emitted by each fiber core can be independently controlled, the complex operations of cell capture, rotation and the like can be completed by only using one multicore fiber.
3. The operation is simple. The structure of the device does not need to be changed, each fiber core can be independently controlled by adjusting the attenuator, and only the output light field of the corresponding optical fiber needs to be controlled when the cell is captured and rotated.
(IV) description of the drawings
FIG. 1 is a schematic diagram of a suspended optical motor system.
Fig. 2 is a schematic view of a multi-core optical fiber.
FIG. 3 is a schematic diagram of a multi-core fiber intermediate core capturing cell.
Fig. 4 is a numbered schematic of each core of a multicore fiber.
FIG. 5 is a schematic view of a cell being rotated about the y-axis using a suspended light motor.
(V) detailed description of the preferred embodiments
The present invention will be described in detail below by taking a seven-core optical fiber suspension type optical motor system as an example.
Example 1: based on the rotation control of seven-core optical fiber suspension type optical motor system to human red blood cells:
FIG. 1 shows a seven-core optical fiber suspension-based optical motor system, which is used for controlling cells and usually uses near infrared light, and in this case, the damage to cell life bodies is small, and the absorption of cells is small, in this embodiment, a 980nm light source is selected. For ease of description, each core is numbered a-f as shown in FIG. 4. 980nm laser light emitted by the laser 1 is divided into 8 laser beams on average through the 1x8 optical fiber beam splitter 3, wherein one laser beam is connected with an optical power meter 9 for measuring the power of the laser light. The other 8 laser beams are respectively connected with attenuators 4-1 to 4-7. The light beam from 4-1 is transmitted to the core a of the seven-core optical fiber 6 through the optical fiber combiner 5. Similarly, the light beam extracted from 4-2 is transmitted to b. And the rest can be done in sequence, so that the light beam of each fiber core in the seven-core optical fiber 6 can be independently controlled by the attenuators 4-1-4-7. The red blood cells are added to the test cuvette and the microscopic imaging system 8 is switched on. Adjusting attenuators 4-2-4-7 to enable fiber cores b-f of the multi-core optical fiber 6 not to emit light fields, and adjusting the attenuators 4-1 to enable the fiber core a to emit proper power to capture red blood cells. As shown in fig. 5. At this time, the attenuator 4-2 is adjusted to make the fiber core b emit an optical field, the kinetic energy transmitted by the optical field acts on the side surface of the red blood cell, the resultant force applied to the red blood cell is zero, and the red blood cell has a rotatable moment. The speed of the red blood cell rotation can be increased or decreased by increasing the power of the optical field in the fiber core b by a proper amount. Similarly, the light fields emitted by different fiber cores are controlled by adjusting the attenuator 4, so that the cells can rotate in different directions. The whole cell captured, rotation controlled image is observed in real time by the microscopic imaging system 8.
Claims (4)
1. A multi-core optical fiber suspension type optical motor system. The optical motor system includes: a laser 1 for generating a desired light source; a single mode optical fiber 2; an optical fiber splitter 3; an attenuator 4; a multi-core optical fiber combiner 5; a multi-core optical fiber 6; a sample cell 7; a microscopic imaging system 8; an optical power meter 9. Laser in the system is emitted from a laser 1 and passes through a standard single-mode fiber 2 to a fiber light splitter 3, and one laser beam is divided into N +1 laser beams. One of the lasers is connected to an optical power meter 9 for measuring the divided laser power. The attenuator 4 comprises N attenuators each connected to a laser beam split from the fiber splitter for individually adjusting the laser power. N optical fibers led out from the attenuator are connected to the input end 6 of the multi-core optical fiber beam combiner, and the multi-core optical fiber beam combiner 5 is connected with the multi-core optical fiber 6. The state of the input laser light to each core in the multicore fiber can be individually controlled by the attenuator 4. The multicore fiber 6 is inserted into the bottom of the experimental sample cell 7, with the fiber perpendicular to the horizontal plane. The test sample cell 7 contains the particles to be manipulated. The attenuator 4 is adjusted, the light field emitted by each fiber core in the multi-core fiber 6 can be independently changed, and the purpose of capturing and rotating particles is achieved. The microscopic imaging system 8 can observe experimental operating conditions in real time. One core of the multicore fiber 6 captures and "lifts" the cell to be manipulated against gravity using optical trapping forces. The power of the optical fields emitted by the different fiber cores is controlled by the attenuator 4, and the scattering force of the attenuator is utilized to enable the cells to have enough rotating torque, so that the particles rotate in different directions. The rotating cellular phase acts as a "rotor" and the laser provides the motive force, forming an optical motor system.
2. The multi-core fiber optic based suspension type optical motor system of claim 1. The multi-core optical fiber used in the system penetrates from the bottom of the sample cell and is vertical to the horizontal plane. A multicore fiber is used to capture and rotate the cells.
3. The multi-core fiber optic based suspension type optical motor system of claim 1. The system adopts multi-core optical fiber, which is characterized in that: the optical fiber has N cores. One of the fiber cores is a middle core, and the other fiber cores are distributed around the middle core in a ring array manner.
4. The multi-core fiber optic based suspension-type optical motor of claim 1. The system achieves the purpose of cell rotation by controlling the fiber core to emit a light field. Each fiber core can independently control whether the optical field is output or not and the power of the output optical field.
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| CN113514442A (en) * | 2021-07-12 | 2021-10-19 | 桂林电子科技大学 | Dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber optical control |
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