CN208701066U - Bacterial clump picking instrument - Google Patents
Bacterial clump picking instrument Download PDFInfo
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- CN208701066U CN208701066U CN201820837291.3U CN201820837291U CN208701066U CN 208701066 U CN208701066 U CN 208701066U CN 201820837291 U CN201820837291 U CN 201820837291U CN 208701066 U CN208701066 U CN 208701066U
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Abstract
The utility model belongs to biomedical self-reacting device technical field, more particularly to a kind of bacterial clump picking instrument, including the rack with pedestal and top plate, the culture dish planar movement platform for being mounted on the base and being used to that culture dish to be driven to make plane motion, it is mounted on the base and is used to acquire the photographic device of the image of the bacterial clump in culture dish, the inoculation orifice plate planar movement platform for being mounted on the base and being used to that inoculation orifice plate to be driven to make plane motion, it is mounted on the base and the rotating device of the disk including that can make to circumferentially rotate, the cleaning sterilizing device for being mounted on the base and being located at below disk periphery, it is mounted on several driving devices that each picking device of the spaced apart several picking devices of periphery and driving on disk and along disk carries out picking movement.Not only picking is high-efficient in the course of work of the utility model, and the accuracy of identification under the cooperation of photographic device for bacterial clump also greatly promotes；Meanwhile instrument overall structure is uncomplicated, it is easy to operate.
Description
Technical field
The utility model belongs to biomedical self-reacting device technical field more particularly to a kind of bacterial clump picking instrument.
Background technique
Currently, the picking of bacterial clump is mainly by craft in the Bioexperiment such as biological gene engineering, microculture
To complete.Increasing with application, a bacterial clump selects experiment and usually needs the thousands of a colonies of picking, work
Work amount is big, it is lower to select efficiency by hand, and select colonies based on subjective judgement, is easy to omit target clone.So by hand
Picking bacterial clump gradually be not able to satisfy high-throughput requirement of experiment.
With the development of biotechnology, traditional-handwork selects bacterial clump and is not able to satisfy wanting for high flux screening gradually
It asks.Instrument is selected instead of selecting bacterial clump automatically by hand using a kind of bacterium, picking efficiency not only can be improved, but also choosing
It takes and is had also been greatly improved in precision.Currently, bacterial clump, which selects instrument, mainly uses 96 needle picking header structures, in X, Y, Z
It can be moved on three rectangular co-ordinate shifting axles.Machine vision technique is used first, with camera to the bacterium bacterium in culture dish
Row identification, positioning are dropped into, then 96 needle picking heads picking colony one by one is once inoculated into inoculation orifice plate, finally chooses needle to 96
Carry out cleaning and sterilizing.
96 needle picking choicest of mainstream at present select instrument there are the shortcomings that: (1) picking is inefficient.96 needle picking header structures are chosen
Selecting picking in mode, inoculation, three steps of disinfection is really to connect to be sequentially completed, and the time of a wheel picking is exactly this three step
Temporal summation.This structure picking mode limits picking efficiency and cannot be greatly improved.(2) structure is complicated.96 needle picking heads
Structure selects mode, 96 needle picking heads as critical component, due to realize each needle can individually activity, interior design compared with
Complexity, and driving method responds slower using pneumatically.(3) accuracy of identification is low.96 needle picking header structures select mode for the ease of
Image and picking are acquired, camera and 96 needle picking heads are fixed together, it means that camera is mobile.It is solid compared to camera
Fixed, the stated accuracy of this mode is lower, and camera has in moving process to and fro and trembles, in this way for finally accurately
Identification bacterium has a significant impact.
Utility model content
The purpose of this utility model is to provide a kind of bacterial clump picking instrument, it is intended to solve 96 needle in the prior art
The technical issues of picking is inefficient, accuracy of identification is low and structure is complicated, is selected existing for instrument in picking choicest.
A kind of in order to achieve the above purposes, the technical solution adopted by the utility model is: bacterial clump picking instrument, comprising:
Rack, including pedestal and the top plate being connected on the pedestal；
Culture dish planar movement platform is installed on the pedestal and for driving culture dish to make plane motion；
Photographic device is installed on the pedestal and is located at the side of the culture dish planar movement platform and for acquiring institute
State the image of the bacterial clump in culture dish；
It is inoculated with orifice plate planar movement platform, is installed on the pedestal and for driving inoculation orifice plate to make plane motion；
Rotating device, is installed on the pedestal and the disk including that can circumferentially rotate and be located at below the top plate；
Cleaning sterilizing device is installed on the pedestal and is located at the lower section of the disk periphery；
Several picking devices, each picking device are mounted on the periphery interval cloth on the disk and along the disk
It sets；
Several driving devices, each driving device are mounted on the top on the top plate and being located at the disk, and
It is dynamic to drive each picking device to carry out picking that each picking device can be corresponded respectively to after the disk rotational angle
Make.
Preferably, the rotating device further includes rotary index device, and the rotary index device is installed in the pedestal
Portion, the disk are fixedly connected with the output end of the rotary index device.
Preferably, the cleaning sterilizing device includes the first rinse bath, the second rinse bath and heater, first cleaning
Slot, second rinse bath and the heater arranged for interval, and each institute can be corresponded respectively to after the disk rotational angle
State picking device.
Preferably, the photographic device includes bracket, video camera and backlight, and the bracket is fixed on the pedestal simultaneously
Positioned at the side of the culture dish planar movement platform, the bracket includes cantilever slab, and the backlight is fixed on the pedestal
And it is located at the lower section of the cantilever slab, the video camera is installed on the cantilever slab and camera lens is arranged towards the backlight.
Preferably, the culture dish planar movement platform includes the linear mould group of the first X-axis, the linear mould group of the first Y-axis and culture
Ware supporting plate, the linear mould group of the first X-axis are installed on the pedestal and arrange along the x axis, the linear mould of the first Y-axis
Group is fixed on the output end of the linear mould group of first X-axis and arranges along the y axis, and the culture dish supporting plate is fixed on institute
On the output end for stating the linear mould group of the first Y-axis.
Preferably, the inoculation orifice plate planar movement platform includes the linear mould group of the second X-axis, the linear mould group of the second Y-axis and connects
Kind orifice plate supporting plate, the linear mould group of the second X-axis are installed on the pedestal and arrange along the x axis, the second Y-axis line
Property mould group is fixed on the output end of the linear mould group of second X-axis and arranges along the y axis, the inoculation orifice plate supporting plate
It is fixed on the output end of the linear mould group of second Y-axis.
Preferably, the picking device includes:
Slide device, including mounting plate, picking executive item, push rod and pressing plate, the mounting plate are described in being vertically fixed on
On the periphery of disk, the picking executive item is slidably connected on the mounting plate, and the pressing plate and the picking executive item connect
Connect and be located at the top of the picking executive item, and the pressing plate extends to the side of the picking executive item, the push rod with
The pressing plate is fixedly connected with and is located at the underface of the direction of motion at the driving end of the driving device；
Resilient mounting, including guide rod, compressed spring, upper mounted plate and bottom plate, fixed plate and it is described under
Fixed plate is fixedly installed on the mounting plate and is respectively positioned on the side of the picking executive item, and fixed plate and institute
Bottom plate is stated in upper and lower arranged for interval, the lower end of the guide rod is fixedly connected with the bottom plate, upper end is across described
Pressing plate is simultaneously fixedly connected with fixed plate, and the compressed spring is sheathed on outside the guide rod, and the compressed spring
Both ends are abutted with the bottom plate and the pressing plate respectively.
Preferably, the resilient mounting further includes cushion, and the cushion is fixed on the guide rod, and described slow
Punching pad is between the pressing plate and fixed plate.
Preferably, the picking device further includes limiting device, and the limiting device is installed on the mounting plate and position
Position between the picking executive item and the guide rod and for limiting the pressing plate.
Preferably, the limiting device includes fixed block and block, and the fixed block is fixedly connected on the mounting plate
And between the picking executive item and the guide rod, the block is connect to decline in the pressing plate with the fixed block
When abut the pressing plate and limit the pressing plate.
Preferably, the limiting device further includes screw rod and two locking nuts, offers threaded hole on the fixed block,
The screw rod is passed through and the threaded hole and is threadedly coupled with the threaded hole, two locking nuts with the screw flight
It connects and is located above and below the threaded hole, the block is fixedly connected on the upper end of the screw rod.
Preferably, the driving device includes double-acting cylinder, magnetic switch and 3 position-5 way solenoid valve, the double acting
Cylinder is fixed on the top on the top plate and being set to the push rod, and the double cropping at the driving end as the driving device
With the upper end setting for stretching out push rod described in the face of direction of the piston rod of cylinder, the magnetic switch is installed on the double acting gas
On cylinder, the 3 position-5 way solenoid valve is connected to inlet end and the outlet side of the double-acting cylinder.
Preferably, the driving device be the cylinder that is installed on the top plate perhaps the linear motor cylinder or
The upper end of push rod described in the output end face of the linear motor is arranged.
Preferably, the picking executive item is to choose needle or suction pipe.
The utility model has the beneficial effects that the bacterial clump picking instrument of the utility model, in use, cultivating respectively first
Culture dish holding and inoculation orifice plate are distinguished on ware planar movement platform and inoculation orifice plate planar movement platform, then pass through culture dish plane
Mobile station driving culture dish is moved to the lower section of photographic device, and the image of the bacterial clump in photographic device acquisition culture dish is simultaneously complete
After identification, positioning, culture dish planar movement platform driving culture dish is moved to the lower section of the periphery of disk, and picking device is driving
Bacterial clump under the driving of device in picking culture dish, then rotating device driving disk circumferentially rotates and drives picking device
It circumferentially rotates, and until picking device is moved to the top of cleaning sterilizing device, at this time driving of the picking device in driving device
Under carry out cleaning and/or heating work, then rotating device drive disk to circumferentially rotate again and drive picking device circumferentially turn
It is dynamic, and until picking device is moved past the top that the inoculation orifice plate of positioning is completed in inoculation orifice plate planar movement platform driving, clearly
The top of wash disinfection device, picking device carries out inoculation work under the drive of the drive at this time；Due to picking device and drive
Dynamic device have it is several and, and one-to-one position can be formed after rotational angle, then each picking device can be real
It now works at the same time, so may be implemented that picking, inoculation, cleaning, the multiple stations of heating are synchronous carries out, in whole work process not only
Picking is high-efficient, and the accuracy of identification under the cooperation of photographic device for bacterial clump also greatly promotes；Meanwhile instrument is whole
Structure is uncomplicated, easy to operate.
Detailed description of the invention
It, below will be to embodiment or the prior art in order to illustrate more clearly of the technical scheme in the embodiment of the utility model
Attached drawing needed in description is briefly described, it should be apparent that, the accompanying drawings in the following description is only that this is practical new
Some embodiments of type for those of ordinary skill in the art without any creative labor, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the schematic perspective view of bacterial clump picking instrument provided by the embodiment of the utility model.
Fig. 2 is another schematic perspective view of bacterial clump picking instrument provided by the embodiment of the utility model.
Fig. 3 is the top view of bacterial clump picking instrument provided by the embodiment of the utility model.
Fig. 4 is that the picking device of bacterial clump picking instrument provided by the embodiment of the utility model and the structure of driving device are shown
It is intended to.
Fig. 5 is the schematic perspective view of the picking device of bacterial clump picking instrument provided by the embodiment of the utility model.
Fig. 6 is the main view of the picking device of bacterial clump picking instrument provided by the embodiment of the utility model.
Fig. 7 is the side view of the picking device of bacterial clump picking instrument provided by the embodiment of the utility model.
Wherein, each appended drawing reference in figure:
10-rack 11-pedestal, 12-top plates
13-linear mould the groups of connecting plate 20-culture dish planar movement the 21-the first X-axis of platform
22-the first Y-axis linear mould group 23-culture dish, 30-photographic device of supporting plate
31-bracket 32-video camera, 33-backlights
40-inoculation linear linear mould the groups of the 42-the second Y-axis of mould group of the 41-the second X-axis of orifice plate planar movement platform
43-inoculation orifice plate supporting plate 50-rotating device, 51-disks
52-rotary index device the 61-the first rinse baths of 60-cleaning sterilizing device
62-the second rinse bath 63-heater, 70-picking device
71-slide device 72-resilient mounting, 73-limiting devices
80-driving device 81-double-acting cylinder, 82-magnetic switch
83-90-culture dish of 3 position-5 way solenoid valve, 100-inoculation orifice plates
311-cantilever slab 711-mounting plate, 712-picking executive items
713-push rod 714-pressing plate, 715-guide rails
716-sliding block 717-mounting blocks, 721-guide rods
722-compressed spring 723-upper mounted plate, 724-bottom plates
725-cushion 731-fixed block, 732-blocks
733-screw rod 734-locking nut, 811-piston rods.
Specific embodiment
The embodiments of the present invention are described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning
Same or similar element or element with the same or similar functions are indicated to same or similar label eventually.Below by ginseng
The embodiment for examining the description of attached drawing 1~7 is exemplary, it is intended to for explaining the utility model, and should not be understood as practical to this
Novel limitation.
In the description of the present invention, it should be understood that term " length ", " width ", "upper", "lower", " preceding ",
The orientation or positional relationship of the instructions such as " rear ", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside" is based on attached
Orientation or positional relationship shown in figure, is merely for convenience of describing the present invention and simplifying the description, rather than indication or suggestion
Signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to this
The limitation of utility model.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include one or more of the features.The meaning of " plurality " is two or two in the description of the present invention,
More than, unless otherwise specifically defined.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " Gu
It is fixed " etc. terms shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral；It can be
Mechanical connection, is also possible to be electrically connected；It can be directly connected, two can also be can be indirectly connected through an intermediary
The interaction relationship of connection or two elements inside element.It for the ordinary skill in the art, can basis
Concrete condition understands the concrete meaning of above-mentioned term in the present invention.
As shown in Figures 1 to 3, a kind of bacterial clump picking instrument provided by the embodiment of the utility model, comprising:
Rack 10, including pedestal 11 and the top plate 12 being connected on the pedestal 11；The setting of pedestal 11 and top plate 12 is equal
The installation for other component and the support to other component are played, pedestal 11 is mainly used for being located below the peace of the component of setting
Dress and support, and top plate 12 is then mainly used for being located above the installation and support of the component of setting.Wherein, pedestal 11 and bottom plate can
With with metal plate.It is connect in addition, top plate 12 passes through the connecting plate 13 arranged vertically with pedestal 11, pedestal 11 and top plate is made with this
Installation accommodating space is formed between 12.
Culture dish planar movement platform 20 is installed on the pedestal 11 and for driving culture dish 90 to make plane motion；Training
Supporting ware planar movement platform 20 is the device that at least can be realized plane X-axis and Y-axis movement, is mainly used for that culture dish 90 is driven to make
The driving of culture dish 90 can be moved in the space in stroke range by plane motion according to real work demand in this way.
Photographic device 30 is installed on the pedestal 11 and is located at the side of the culture dish planar movement platform 20 and is used for
Acquire the image of the bacterial clump in the culture dish 90；Photographic device 30 can realize image and acquire, then being taken the photograph by this
As high-precision can may be implemented according to the image of acquisition to the progress Image Acquisition of the bacterial clump in culture dish 90 in device 30
Positioning, wherein culture dish 90 can be moved to the camera shooting model of photographic device 30 under the driving of culture dish planar movement platform 20
Interior carry out Image Acquisition is enclosed, after completing Image Acquisition, culture dish 90 drives through culture dish planar movement platform 20 again and is moved to down
The station of one working link.
It is inoculated with orifice plate planar movement platform 40, is installed on the pedestal 11 and for driving inoculation orifice plate 100 to make plane fortune
It is dynamic；Inoculation orifice plate planar movement platform 40 is the device that at least can be realized plane X-axis and Y-axis movement, is mainly used for driving and connects
Kind orifice plate 100 makees plane motion, the inoculation driving of orifice plate 100 can be moved to stroke range according to real work demand in this way
In interior space.The inoculation orifice plate planar movement platform 40 it is identical as the structure that above-mentioned culture dish planar movement platform 20 uses or
It is similar, and it is respectively separated setting, such as can be set in the left and right sides of entire apparatus subject.Wherein, inoculation orifice plate 100 is excellent
It is selected as 96 orifice plates.
Rotating device 50 is installed on the pedestal 11 and including that can circumferentially rotate and be located at 12 lower section of top plate
Disk 51；Specifically, the main function of rotating device 50 is that the disk 51 of its own is driven to circumferentially rotate.It can on disk 51
To set multiple stations such as virtual picking, inoculation, cleaning, heating, it can be determined with this and be mounted on corresponding work on disk 51
Realize corresponding work in position.
Cleaning sterilizing device 60 is installed on the pedestal 11 and is located at the lower section of 51 periphery of disk；Cleaning and sterilizing
The executive item that device 60 carries out picking for cleaning and sterilizing, is inoculated with work.
Several picking devices 70, each picking device 70 are mounted on the disk 51 and along the week of the disk 51
Edge arranged for interval；Picking device 70 is that executive device is held when the axial direction with disk 51 turns to corresponding work station
Row is accordingly to work such as the picking of bacterial clump, inoculation, cleaning, heating.
Several driving devices 80, each driving device 80 are mounted on the top plate 12 and are located at the disk 51
Top, and each picking device 70 can be corresponded respectively to after 51 rotational angle of disk to drive each picking dress
Set 70 carry out picking movements.Driving device 80 be mainly used for drive picking device 70 execute to the picking of bacterial clump, inoculation,
The work such as cleaning, heating.Driving device 80, which can be, to be connected installation with picking device 70 or separates non-contact installation.
Specifically, the bacterial clump picking instrument of the utility model embodiment, in use, being moved respectively in culture dish plane first
Culture dish holding 90 and inoculation orifice plate 100 are distinguished on dynamic platform 20 and inoculation orifice plate planar movement platform 40, it is then flat by culture dish
The driving culture dish 90 of face mobile station 20 is moved to the lower section of photographic device 30, and photographic device 30 acquires the bacterium bacterium in culture dish 90
The image that falls and after completing identification, positioning, culture dish planar movement platform 20 drive culture dish 90 to be moved to the periphery of disk 51
Lower section, bacterial clump of the picking device 70 under the driving of driving device 80 in picking culture dish 90, then rotating device 50 drives
Dynamic disk 51 circumferentially rotates and picking device 70 is driven to circumferentially rotate, and until picking device 70 is moved to cleaning sterilizing device
60 top, picking device 70 carries out cleaning and/or heating work under the driving of driving device 80 at this time, then rotating device
50 drive disk 51 to circumferentially rotate and picking device 70 is driven to circumferentially rotate again, and until picking device 70 is moved past and connects
The top of the inoculation orifice plate 100 of the kind driving completion positioning of orifice plate planar movement platform 40, the top of cleaning sterilizing device 60, at this time
Picking device 70 carries out inoculation work under the driving of driving device 80；Due to picking device 70 and driving device 80 have it is several
And and one-to-one position can be formed after rotational angle, then each picking device 70 may be implemented to work at the same time, such as
This may be implemented that picking, inoculation, cleaning, the multiple stations of heating are synchronous carries out, and not only picking is high-efficient in whole work process, phase
Compare 96 needle picking header structures, picking efficiency almost improves three times, and bacterial clump is directed under the cooperation of photographic device 30
Accuracy of identification also greatly promote；Meanwhile instrument overall structure is uncomplicated, it is easy to operate.
In the present embodiment, as shown in Figure 1, the rotating device 50 further includes rotary index device 52, the rotary index device
52 are installed on the middle part of the pedestal 11, and the disk 51 is fixedly connected with the output end of the rotary index device 52.Specifically,
Rotary index device 52 can be used for that disk 51 is driven to rotate, and the angle that can be rotated with real-time control, to ensure each picking dress
The lower section that can correspond to a driving device 80 after 70 rotational angles is set, can so enable driving device 80 to control and choose
Device 70 is taken to work.
In the present embodiment, as shown in figures 2-3, the cleaning sterilizing device 60 includes the first rinse bath 61, the second rinse bath
62 and heater 63,63 arranged for interval of first rinse bath 61, second rinse bath 62 and the heater, and described
Each picking device 70 can be corresponded respectively to after 51 rotational angle of disk.Specifically, steaming can be added in the first rinse bath 61
The picking executive item 712 of distilled water or other cleaning solutions cleaning picking device 70, can add 70% alcohol in the second rinse bath 62
Picking executive item 712 is cleaned, heater 63 is for heating picking executive item 712, high-temperature sterilization.
Wherein, the scale division value (spacing) between 63 three of the first rinse bath 61, the second rinse bath 62 and heater, which is equal to, appoints
The scale division value (spacing) of three adjacent picking devices 70 of meaning.So may insure that there are three picking devices 70 can be simultaneously the
Corresponding work is executed in one rinse bath 61, the second rinse bath 62 and heater 63.
In the present embodiment, as shown in Figures 1 to 3, the photographic device 30 includes bracket 31, video camera 32 and backlight 33,
The bracket 31 is fixed on the side on the pedestal 11 and being located at the culture dish planar movement platform 20, and the bracket 31 wraps
Cantilever slab 311 is included, the backlight 33 is fixed on the lower section on the pedestal 11 and being located at the cantilever slab 311, the camera shooting
Machine 32 is installed on the cantilever slab 311 and camera lens is arranged towards the backlight 33.Specifically, the cantilever slab 311 of bracket 31
Setting may insure that the video camera 32 being mounted thereon can be vacantly in certain spatial position, so can be in order to making
The camera lens of video camera 32 can face backlight 33 and then can be improved with the effect of this acquisition image for promoting video camera 32
Acquire the quality of image.
In the present embodiment, as shown in Figures 1 to 3, the culture dish planar movement platform 20 include the linear mould group 21 of the first X-axis,
The linear mould group 22 of first Y-axis and culture dish supporting plate 23, the linear mould group 21 of the first X-axis be installed on the pedestal 11 and
It arranges along the x axis, the linear mould group 22 of the first Y-axis is fixed on the output end of the linear mould group 21 of first X-axis and along Y
Axis direction arrangement, the culture dish supporting plate 23 are fixed on the output end of the linear mould group 22 of first Y-axis.Specifically,
The movement of X-direction may be implemented in the driving end of the linear mould group 21 of one X-axis, and the driving end of the linear mould group 22 of the first Y-axis then may be used
To realize the movement of Y direction, in this way, driving the linear mould group 22 of the first Y-axis to move along X-axis by the linear mould group 21 of the first X-axis
Culture dish supporting plate 23 is driven to move realization driving culture dish supporting plate 23 along X along Y-axis in conjunction with by the linear mould group 22 of the first Y-axis
The plane motion of axis and Y-axis, then the culture dish 90 being placed on culture dish supporting plate 23 then may be implemented along X-axis and Y-axis
Plane motion can drive culture dish 90 to be moved to suitable position according to actual needs and be worked accordingly.
In the present embodiment, as shown in figs. 1 and 3, the inoculation orifice plate planar movement platform 40 includes the linear mould group of the second X-axis
41, the linear mould group 42 of the second Y-axis and inoculation orifice plate supporting plate 43, the linear mould group 41 of the second X-axis are installed on the pedestal 11
It above and along the x axis arranges, the linear mould group 42 of the second Y-axis is fixed on the output end of the linear mould group 41 of second X-axis
And arrange along the y axis, the inoculation orifice plate supporting plate 43 is fixed on the output end of the linear mould group 42 of second Y-axis.Tool
Body, the driving end of the linear mould group 41 of the second X-axis may be implemented the movement of X-direction, and the driving of the linear mould group 42 of the second Y-axis
The movement of Y direction then may be implemented in end, in this way, driving the 2nd Y axis mould group 42 along X by the linear mould group 41 of the second X-axis
Axis movement, which is combined, moves realization driving inoculation orifice plate along Y-axis by the linear driving of the mould group 42 inoculation orifice plate supporting plate 43 of the second Y-axis
Supporting plate 43 along X-axis and Y-axis plane motion, then the inoculation orifice plate 100 being placed on inoculation orifice plate supporting plate 43 then can be with
It realizes the plane motion along X-axis and Y-axis, inoculation orifice plate 100 can be driven to be moved to suitable position according to actual needs and carried out
Corresponding work.
Preferably, the linear mould group 21 of the first X-axis, the linear mould group 22 of the first Y-axis, the linear mould group 41 of the second X-axis and the second Y-axis
The structure of linear mould group 42 is all the same, is that difference is controlled in spatial position in specific installation.The wherein linear mould group of the first X-axis
21, the linear mould group 22 of the first Y-axis, the linear mould group 41 of the second X-axis and the linear mould group 42 of the second Y-axis include shell, are set to shell
It is interior and along gas length arrangement and rotatable screw rod, be threadedly connected to screw rod travelling nut, be fixedly connected with travelling nut simultaneously
Shell is exposed outside and the slide plate as driving end and the motor that connect in shell with wherein one end of screw rod, in this way, electric
Machine rotation drives screw rod rotation, drives travelling nut straight line (X-axis or Y-axis) mobile, is fixedly connected on the culture dish 90 of slide plate
Either X-axis or Y-axis movement can be realized in inoculation orifice plate 100.In general, the first X axis mould group 21, the linear mould group of the first Y-axis
22, the linear mould group 41 of the second X-axis and the linear mould group 42 of the second Y-axis further include playing the sliding rail of guiding and supporting role or leading
To axis and hole.Certainly, the cooperation of screw rod and travelling nut can also be replaced by belt and belt pulley, not gone to live in the household of one's in-laws on getting married herein
It states.
In the present embodiment, as also shown in e.g. figs. 4-7, the picking device 70 includes slide device 71 and resilient mounting 72；Sliding
Device 71 includes mounting plate 711, picking executive item 712, push rod 713 and pressing plate 714, and the picking executive item 712 is slidably connected
In on the mounting plate 711, the pressing plate 714 connect with the picking executive item 712 and is located at the picking executive item 712
Top, and the pressing plate 714 extends to the side of the picking executive item 712, the push rod 713 and the pressing plate 714 are fixed
Connect and be located at the underface of the direction of motion at the driving end of the driving device 80；Resilient mounting 72 includes guide rod 721, pressure
The fixed peace of contracting spring 722, upper mounted plate 723 and bottom plate 724, fixed plate 723 and the bottom plate 724
Loaded on the mounting plate 711 and being respectively positioned on the side of the picking executive item 712, and fixed plate 723 and it is described under
Fixed plate 724 is in upper and lower arranged for interval, and the lower end of the guide rod 721 is fixedly connected with the bottom plate 724, upper end passes through
The pressing plate 714 is simultaneously fixedly connected with fixed plate 723, and the compressed spring 722 is sheathed on outside the guide rod 721,
And the both ends of the compressed spring 722 are abutted with the bottom plate 724 and the pressing plate 714 respectively.When specifically used, driving
The driving end of device 80 moves downward until abutting push rod 713, and push rod 713 moves downward and pushes pressing plate connected to it
714 move downward, and pressing plate 714 drives picking executive item 712 to move downward, and picking executive item 712 holds corresponding movement, passes through drive
Dynamic device 80 can accurately control the movement of picking executive item 712, also, driving device 80 is to separate to set with picking device 70
After the single that meter, i.e. driving device 80 complete control picking executive item 712 moves downward execution movement, retracts automatically and continue to keep
The state isolated with picking device 70；Since pressing plate 714 is during moving downward, 712 side of picking executive item is extended to
The position of side is simultaneously abutted with the compressed spring of resilient mounting 72 722 always, then pressing plate 714 can compress the compression bullet simultaneously
Spring 722 forces pressing plate 714 under the action of the screen resilience of compressed spring 722 after the control that driving device 80 applies is exited
It is to move upwards with guide rod 721, pressing plate 714 drives picking executive item 712 to move upwards simultaneously, so realizes automatic return
It resets, in this way, picking executive item 712, which can be realized, automatically replies reset, driving device 80 may be implemented to control multiple pickings
The picking executive item 712 of device 70 acts, i.e. 80 position of driving device is fixed, then it can control any one with circle
Disk 51 circumferentially rotates the work of the picking device 70 to 80 lower section of driving device, and the positioning accuracy that whole device is realized is high, structure
Simply and using more flexible.
In the present embodiment, picking executive item 712 is to realize the component for executing relevant action, it is preferable that the picking executes
Part 712 is to choose needle or suction pipe.In this way, picking and liquid relief can be realized respectively by choosing needle or suction pipe as picking executive item 712
Movement, so can be used for biological medicine experiment in sample extraction or transfer.
Further, the tip diameter for choosing needle is 1mm, and upper body diameter is about 4mm and no more than 6mm, and material can be with
Using heating resisting metal.
In the present embodiment, as shown in figs. 1 and 4, the driving device 80 includes double-acting cylinder 81, magnetic switch 82 and three
Position five-way electromagnetic valve 83, the double-acting cylinder 81 is fixed on the top on the top plate 12 and being set to the push rod 713, and makees
Push rod described in stretching direction face for the piston rod 811 of the double-acting cylinder 81 at the driving end of the driving device 80
713 upper end setting, the magnetic switch 82 are installed on the double-acting cylinder 81, and the 3 position-5 way solenoid valve 83 connects
Inlet end and outlet side in the double-acting cylinder 81.Specifically, magnetic switch 82 is used to detect the work of double-acting cylinder 81
The position of plug, 3 position-5 way solenoid valve 83 can control air inlet, outlet, the pressure maintaining of double-acting cylinder 81, realize double-acting cylinder
81 stopping at an arbitrary position, the push rod 713 that double-acting cylinder 81 can control picking device 70 move down.
Preferably, double-acting cylinder 81 is in the surface of push rod 713, and the two is at a distance of 10mm or so.
More specifically, in practical applications, magnetic switch 82 and 3 position-5 way solenoid valve 83 are also required to and extraneous control
Center processed is electrically connected, and control centre, which for example can be PLC controller or computer etc. the interaction of information may be implemented, is
System.That is, magnetic switch 82 detects the position of piston after the piston of double-acting cylinder 81 is moved to the position of requirement, letter is issued
Number control centre is given, controller center 3 position-5 way solenoid valve 83 1 instructions, 3 position-5 way solenoid valve 83 completes commutation, real
The accurate positionin of the piston of existing double-acting cylinder 81.
In other embodiments, driving device 80 can also be the cooperation lead screw such as cylinder or linear motor and conveyer belt group
At.
In the present embodiment, as also shown in e.g. figs. 4-7, the resilient mounting 72 further includes cushion 725, and the cushion 725 is solid
Due on the guide rod 721, and the cushion 725 is between the pressing plate 714 and fixed plate 723.Specifically
Ground, when pressing plate 714 moves up and down under the resilient force of compressed spring 722, pressing plate 714 is connected to the lower end of cushion 725,
Due to cushion 725 have buffering performance, so can to avoid pressing plate 714 directly with 723 hard collision of upper mounted plate, not only
It may insure that the operation of whole device is more steady, and pressing plate 714 or upper mounted plate 723 avoided to damage.
Preferably, cushion 725 can be silica gel cushion block or rubber cushion blocks, and silica gel cushion block and rubber cushion blocks all have good
Good toughness can realize flexible contact with pressing plate 714, buffer pressing plate 714 under the resilient force of compressed spring 722
Impact force.
In the present embodiment, as also shown in e.g. figs. 4-7, the slide device 71 further includes guide rail 715 and sliding block 716, the guide rail
715 are fixedly installed on the mounting plate 711 and are arranged in parallel with the guide rod 721, and the sliding block 716 is slidably connected to institute
It states on guide rail 715, the picking executive item 712 and the pressing plate 714 are connect with the sliding block 716.Specifically, guide rail 715
Setting be to realize the guiding of picking executive item 712, and the setting of sliding block 716 is then played the role of support and connected, i.e., and picking
Executive item 712, pressing plate 714 and push rod 713 connect.Sliding block 716 is slided along the length direction of guide rail 715, and sliding route is steady
Fixed, the positioning accuracy of the available guarantee of mobile accuracy, such whole device can be guaranteed.
In the present embodiment, as also shown in e.g. figs. 4-7, the slide device 71 further includes mounting blocks 717, and the mounting blocks 717 are solid
Surely it is connected on the sliding block 716, the pressing plate 714 is fixedly connected on the upper end of the mounting blocks 717, the mounting blocks 717
Corresponding upper installing hole (not shown) and lower mounting hole (not shown) up and down are offered, the lower end of the push rod 713 passes through described
Pressing plate 714 is simultaneously plugged and fixed in the upper installing hole, and the upper end of the picking executive item 712 is plugged and fixed in the lower installation
In hole.Specifically, the setting of mounting blocks 717 can be installed for the connection of picking executive item 712, pressing plate 714 and push rod 713,
It connects so that picking executive item 712, pressing plate 714 and push rod 713 are connect with sliding block 716, also, the setting of mounting blocks 717 can be with
So that entire slide device 71 has broader installation space, that is, the spatial position for increasing 717 thickness direction of mounting blocks supplies
The layout and connection of remaining part.In this way, push rod 713 pushes under the action of driving device 80, pressing plate 714 and mounting blocks 717
It realizes under the action of sliding block 716 and is moved downward along guide rail 715 simultaneously, picking executive item 712 is moved with mounting blocks 717
It realizes and corresponding executes movement.
In the present embodiment, the first via hole (not shown) and confession worn for the push rod 713 is offered on the pressing plate 714
The second via hole (not shown) that the guide rod 721 is worn.Specifically, the setting of the first via hole is worn for push rod 713 to push away
Bar 713 can be plugged and fixed with the upper installing hole of mounting blocks 717.The setting of second via hole ensures between pressing plate 714 and guide rod 721
Gap cooperation, such pressing plate 714 can be realized along guide plate up and down motion compresses compressed spring 722 and in compressed spring 722
Active force under move upwards.
In the present embodiment, as also shown in e.g. figs. 4-7, the picking device 70 further includes limiting device 73, the limiting device 73
It is installed on the mounting plate 411 and between the picking executive item 712 and the guide rod 721 and for limiting institute
State the position of pressing plate 714.Specifically, limiting device 73 can limit for mechanical structure, and the minimum decline of pressing plate 714 is ensured with this
Position promotes the positioning accuracy of picking executive item 712.
Preferably, the limiting device 73 includes fixed block 731 and block 732, and the fixed block 731 is fixedly connected on institute
It states on mounting plate 711 and between the picking executive item 712 and the guide rod 721, the block 732 and the fixation
The connection of block 731 is to abut the pressing plate 714 when the pressing plate 714 decline and limit the pressing plate 714.Specifically, limit dress
The effect for setting 73 is the stroke for limiting picking executive item 712 and moving downward.That is, after pressing plate 714 moves downward certain height, pressure
Plate 714 can be connected on the block 732 connecting with fixed block 731, and such block 732, which has just limited pressing plate 714, to be continued downwards
The limit to pressing plate 714 is realized in movement, and picking executive item 712 is to decline indirectly with the decline of pressing plate 714, in this way
After pressing plate 714 is limited, picking executive item 712 is also limited, and can so realize the decline of setting picking executive item 712
Stroke further ensures that the accurate positioning to picking executive item 712.
In the present embodiment, as also shown in e.g. figs. 4-7, the limiting device 73 further includes screw rod 733 and two locking nuts 734,
Offer threaded hole (not shown) on the fixed block 731, the screw rod 733 pass through the threaded hole and with the threaded hole spiral shell
Line connection, two locking nuts 734 be threadedly coupled with the screw rod 733 and be located at the threaded hole top and
Lower section, the block 732 are fixedly connected on the upper end of the screw rod 733.Specifically, screw rod 733 and two locking nuts 734 are total
With the adjusting realized to 732 height and position of block, the threaded hole opened up on screw rod 733 and fixed block 731 is threadedly coupled, and is revolved in this way
When turning screw rod 733, the straight-line displacement of short transverse is may be implemented in screw rod 733, in this way, being connected to the block of the upper end of screw rod 733
732 also realize that the adjusting of height and position tightens two locking nuts 734 i.e. after completing to the adjusting of the height and position of block 732
Lockable screw rod 733 avoids screw rod 733 from upper and lower displacement occur, is so achieved that the adjusting of the height and position of block 732, into
And the restriction of the descending stroke to picking executive item 712 is realized, realize the accurate positioning of picking executive item 712.
The picking device 70 of the utility model is located through electrical Location (3 position-5 way solenoid valve 83 and magnetic switch
82) it is combined with machinery positioning (block 732), so realizes high-precision positioning
In other embodiments, the driving device 80 is the cylinder or linear motor being installed on the top plate 12,
The upper end of push rod 713 described in the output end face of the cylinder or the linear motor is arranged.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Made any modifications, equivalent replacements, and improvements etc., should be included in the utility model within the spirit and principle of utility model
Protection scope within.
Claims (14)
1. a kind of bacterial clump picking instrument, it is characterised in that: include:
Rack, including pedestal and the top plate being connected on the pedestal；
Culture dish planar movement platform is installed on the pedestal and for driving culture dish to make plane motion；
Photographic device is installed on the pedestal and is located at the side of the culture dish planar movement platform and for acquiring the training
Support the image of the bacterial clump in ware；
It is inoculated with orifice plate planar movement platform, is installed on the pedestal and for driving inoculation orifice plate to make plane motion；
Rotating device, is installed on the pedestal and the disk including that can circumferentially rotate and be located at below the top plate；
Cleaning sterilizing device is installed on the pedestal and is located at the lower section of the disk periphery；
Several picking devices, each picking device are mounted on the periphery arranged for interval on the disk and along the disk；
Several driving devices, each driving device are mounted on the top on the top plate and being located at the disk, and in institute
Each picking device can be corresponded respectively to after stating disk rotational angle to drive each picking device to carry out picking movement.
2. bacterial clump picking instrument according to claim 1, it is characterised in that: the rotating device further includes rotary index
Device, the rotary index device are installed on the middle part of the pedestal, the fixed company of the output end of the disk and the rotary index device
It connects.
3. bacterial clump picking instrument according to claim 1, it is characterised in that: the cleaning sterilizing device includes first clear
Washing trough, the second rinse bath and heater, first rinse bath, second rinse bath and the heater arranged for interval, and
Each picking device can be corresponded respectively to after the disk rotational angle.
4. bacterial clump picking instrument according to claim 1, it is characterised in that: the photographic device includes bracket, camera shooting
Machine and backlight, the bracket are fixed on the side on the pedestal and being located at the culture dish planar movement platform, the bracket
Including cantilever slab, the backlight is fixed on the lower section on the pedestal and being located at the cantilever slab, and the video camera is installed on
On the cantilever slab and camera lens is arranged towards the backlight.
5. bacterial clump picking instrument according to claim 1, it is characterised in that: the culture dish planar movement platform includes the
The linear mould group of one X-axis, the linear mould group of the first Y-axis and culture dish supporting plate, the linear mould group of the first X-axis are installed on the pedestal
It above and along the x axis arranges, the linear mould group of the first Y-axis is fixed on the output end of the linear mould group of first X-axis and along Y
Axis direction arrangement, the culture dish supporting plate are fixed on the output end of the linear mould group of first Y-axis.
6. bacterial clump picking instrument according to claim 1, it is characterised in that: the inoculation orifice plate planar movement platform includes
The linear mould group of second X-axis, the linear mould group of the second Y-axis and inoculation orifice plate supporting plate, the linear mould group of the second X-axis are installed on described
It arranges on pedestal and along the x axis, the linear mould group of the second Y-axis is fixed on the output end of the linear mould group of second X-axis
And arrange along the y axis, the inoculation orifice plate supporting plate is fixed on the output end of the linear mould group of second Y-axis.
7. described in any item bacterial clump picking instrument according to claim 1~6, it is characterised in that: the picking device includes:
Slide device, including mounting plate, picking executive item, push rod and pressing plate, the mounting plate are in vertically to be fixed on the disk
Periphery on, the picking executive item is slidably connected on the mounting plate, and the pressing plate is connect simultaneously with the picking executive item
Positioned at the top of the picking executive item, and the pressing plate extends to the side of the picking executive item, the push rod with it is described
Pressing plate is fixedly connected with and is located at the underface of the direction of motion at the driving end of the driving device；
Resilient mounting, including guide rod, compressed spring, upper mounted plate and bottom plate, fixed plate and the lower fixation
Plate is fixedly installed on the mounting plate and is respectively positioned on the side of the picking executive item, and fixed plate and it is described under
Fixed plate is in upper and lower arranged for interval, and the lower end of the guide rod is fixedly connected with the bottom plate, upper end passes through the pressing plate
And be fixedly connected with fixed plate, the compressed spring is sheathed on outside the guide rod, and the both ends of the compressed spring
It is abutted respectively with the bottom plate and the pressing plate.
8. bacterial clump picking instrument according to claim 7, it is characterised in that: the resilient mounting further includes cushion,
The cushion is fixed on the guide rod, and the cushion is between the pressing plate and fixed plate.
9. bacterial clump picking instrument according to claim 7, it is characterised in that: the picking device further includes limit dress
It sets, the limiting device is installed on the mounting plate and between the picking executive item and the guide rod and for limiting
Make the position of the pressing plate.
10. bacterial clump picking instrument according to claim 9, it is characterised in that: the limiting device include fixed block and
Block, the fixed block are fixedly connected on the mounting plate and between the picking executive item and the guide rods, institute
Block is stated to be connect with the fixed block to abut the pressing plate when pressing plate decline and limit the pressing plate.
11. bacterial clump picking instrument according to claim 10, it is characterised in that: the limiting device further include screw rod and
Two locking nuts, offer threaded hole on the fixed block, the screw rod pass through the threaded hole and with the threaded hole spiral shell
Line connection, two locking nuts connect with the screw flight and are located above and below the threaded hole,
The block is fixedly connected on the upper end of the screw rod.
12. bacterial clump picking instrument according to claim 7, it is characterised in that: the driving device includes double acting gas
Cylinder, magnetic switch and 3 position-5 way solenoid valve, the double-acting cylinder are fixed on the top plate and are set to the upper of the push rod
Side, and push rod described in the stretching direction face of the piston rod of the double-acting cylinder at the driving end as the driving device
Upper end setting, the magnetic switch are installed on the double-acting cylinder, and the 3 position-5 way solenoid valve is connected to the double cropping
Inlet end and outlet side with cylinder.
13. bacterial clump picking instrument according to claim 7, it is characterised in that: the driving device is described to be installed on
The upper end of cylinder on top plate perhaps push rod described in the output end face of the linear motor cylinder or the linear motor is set
It sets.
14. bacterial clump picking instrument according to claim 7, it is characterised in that: the picking executive item is to choose needle or suction
Pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820837291.3U CN208701066U (en) | 2018-05-31 | 2018-05-31 | Bacterial clump picking instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820837291.3U CN208701066U (en) | 2018-05-31 | 2018-05-31 | Bacterial clump picking instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208701066U true CN208701066U (en) | 2019-04-05 |
Family
ID=65935698
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820837291.3U Active CN208701066U (en) | 2018-05-31 | 2018-05-31 | Bacterial clump picking instrument |
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| Country | Link |
|---|---|
| CN (1) | CN208701066U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441417A (en) * | 2018-05-31 | 2018-08-24 | 深圳先进技术研究院 | Bacterial clump picking instrument |
| WO2023005106A1 (en) * | 2021-07-26 | 2023-02-02 | 中国科学院深圳先进技术研究院 | Automatic reset mechanism for pick-up needle and automatic colony pick-up instrument |
| WO2023005107A1 (en) * | 2021-07-26 | 2023-02-02 | 中国科学院深圳先进技术研究院 | Automatic colony picking instrument and picking method therefor |
-
2018
- 2018-05-31 CN CN201820837291.3U patent/CN208701066U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441417A (en) * | 2018-05-31 | 2018-08-24 | 深圳先进技术研究院 | Bacterial clump picking instrument |
| CN108441417B (en) * | 2018-05-31 | 2024-01-16 | 深圳先进技术研究院 | Bacterial colony picking instrument |
| WO2023005106A1 (en) * | 2021-07-26 | 2023-02-02 | 中国科学院深圳先进技术研究院 | Automatic reset mechanism for pick-up needle and automatic colony pick-up instrument |
| WO2023005107A1 (en) * | 2021-07-26 | 2023-02-02 | 中国科学院深圳先进技术研究院 | Automatic colony picking instrument and picking method therefor |
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