CN208631136U - A kind of multicolor fluorescence immunohistochemical kit - Google Patents

Abstract

The utility model provides a kind of multicolor fluorescence immunohistochemical kit, it includes box body and box cover, the box body is cylinder, box cover can enclosed box body in a rotative pattern, the partition of hole is set in the box body containing two layers, each layer of partition has certain thickness, box body is divided into upper layer and lower layer by two layers of partition, wherein there are three macropores for tool in lower layer's partition, and upper layer partition has multiple apertures, two two centers of circle separated in multiple apertures with upper layer in three macropores of lower layer's partition are respectively on same vertical axis.To effectively prevent separating the change of mutual alignment up and down, the setting of multiple apertures allows various reagents bottle to consolidate setting, to prevent the random of reagent from shaking, falling down, avoids that reagent is leaked due to toppling over or bottle body is broken.The box body setting of black non transparent can prevent the photomodification of reagent.

Description

A kind of multicolor fluorescence immunohistochemical kit

Technical field

The utility model relates to field of biotechnology, and in particular to a kind of multicolor fluorescence immunohistochemical kit.

Background technique

Immunohistochemistry is also known as immunocytochemistry, refers to the specific antibody with color developing agent label in histocyte original Position passes through antigen-antibody reaction and histochemical color reaction, one for carrying out qualitative, positioning to corresponding antigens, quantitative determining New technology.It dexterously combines specific, the histochemical visibility of immune response, (including glimmering by microscope Light microscope, electron microscope) imaging and amplification, detect various antigenic substance (such as albumen in cell, subcellsular level Matter, polypeptide, enzyme, hormone, pathogen and receptor etc.).Multicolor fluorescence technology is a set of based on junket amide signal amplification technique Multiple labeling immunohistochemical staining scheme is contaminated for tissue sample especially FFPE (the fixed paraffin embedding of formalin) sample Color, and allow to carry out multiple labeling complex staining using the different primary antibodies of same Species origin in same sample.Cooperate multispectral Image analysis system, can obtain the image of high s/n ratio (background correction autofluorescence), even up to 7-10 kind label Simultaneously dyeing and common location and quantitative analysis.Antibody involved in the technology, dyestuff and auxiliary reagent are more.And it is existing immune Groupization kit mainly uses the form of different carton packages, and the reagent bottle for containing reagent and specification are placed directly in packaging In box, that there are disadvantages is obvious for setting in this way.In transportational process, it is easy to topple over and mutually collides, square carton is placed in refrigerator Middle duty is larger and moisture-sensitive deforms.And reagent price used in multicolor fluorescence technology is more expensive, it is desirable that kit Packaging is improved to adapt to the demand of the multicolor fluorescence immunohistochemical kit of high precision.

Utility model content

To solve the above problems, the purpose of this utility model is to propose a kind of improved multicolor fluorescence immunohistochemistry reagent Box.

The technical solution of the utility model is as follows: a kind of multicolor fluorescence immunohistochemical kit comprising box body and box cover, The box body is cylinder, box cover can enclosed box body in a rotative pattern, the partition of hole is set in the box body containing two layers, Each layer of partition has certain thickness, and box body is divided into upper layer and lower layer by two layers of partition, wherein in lower layer's partition there are three tools Macropore, and upper layer partition has multiple apertures, two in three macropores of lower layer's partition are separated in multiple apertures with upper layer Two centers of circle respectively on same vertical axis.

Further, place two reagent bottles in two macropores of above-mentioned lower layer partition, two reagent bottles present it is upper it is narrow under Wide platform columnar structures, bottle cap expose from the coaxial aperture that upper layer separates, to make upper layer partition by this two Reagent bottle is firm to be shelved in lower layer's partition.

Further, two macropores of lower layer's partition place the biggish examination of the dosages such as secondary antibody kit and confining liquid respectively Agent, and another macropore places signal and amplifies liquid reagent.

Further, other apertures of upper layer partition place different fluorescent dye or antibody reagent and mounting respectively Agent.

Further, the box body and box cover are black non transparent material.

The effect of the utility model is: the utility model provides a kind of immunohistochemical kit, bilevel design So that kit is placed more reagent bottles, separates the design of coaxial aperture up and down so that upper and lower partition was consolidated by reagent bottle It links together, the change of partition mutual alignment up and down can be effectively prevented, the setting of multiple apertures makes various reagents bottle can be with Firm setting avoids that reagent is leaked due to toppling over or bottle body is broken to prevent arbitrarily shaking, fall down for reagent.Modeling Expect material, can be placed in refrigerator and be deformed without making moist.The box body setting of black non transparent can prevent photaesthesia reagent Photomodification.

Detailed description of the invention

Fig. 1 is kit outside drawing；

Fig. 2 is lower layer's partition；

Fig. 3 is upper layer partition；

Fig. 4 is that a kind of lower layer of specific size separates；

Fig. 5 is that a kind of upper layer of specific size separates.

Specific embodiment

It is practical new to this below in conjunction with attached drawing to keep the purpose of this utility model, technical solution and advantage clearer Type embodiment is described in further detail.

The outside drawing of kit, including box body and box cover is shown in Fig. 1, using plastic material, can be placed on refrigerator In without make moist deform.Using black non transparent material as box body, the photomodification of reagent can be prevented.Using cylindrical body knot Structure effectively increases the box strength of kit.Lower layer's partition is shown in Fig. 2, and it can be it that partition, which has certain thickness, In reagent bottle provide support strength.Thereon there are three macropore, it is respectively used to place two antiantibodys, confining liquid, signal amplification liquid. Upper layer partition is shown in Fig. 3, has multiple apertures, and the lower layer separates in three macropores two multiple small with upper layer partition Two centers of circle in hole are respectively on same vertical axis, i.e. hole A1 and the center of circle of hole B1 are concentric, the circle of hole A2 and hole B2 The heart is concentric.Setting in this way, when placing two higher reagent bottles in two macropores of hole B1 and B2, bottle cap and portion Sub-bottle body will be more than that upper layer separates, and exposes from hole A1 and A2, since up-narrow and down-wide important actor type is presented in two big reagent bottle Structure, when exposing in the hole A1 and A2 that its bottle cap separates from upper layer, by the rational design of bottle cap and bottleneck size and radius, Upper layer partition can be made to be shelved in lower layer's partition by the two reagent bottles.Both it ensure that between upper layer partition and lower layer's partition The fixation of mutual alignment, and overall structure can be made to keep stablizing, so that prevent reagent bottle topples over collision.What Fig. 4 and Fig. 5 was shown It is a kind of partition design of specific pore size.Two macropores of lower layer's partition place secondary antibody antibody reagent and confining liquid examination respectively Agent, and another macropore places signal and amplifies liquid reagent.Other apertures of upper layer partition place fluorescent dye, an antiantibody respectively With the parcels installed reagents such as mountant.

Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention, it is not limited to this Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art For, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic It is equivalently replaced.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on, It should be included within the scope of protection of this utility model.

Claims (6)

1. a kind of multicolor fluorescence immunohistochemical kit comprising box body and box cover, the box body are cylinder, and box cover can revolve Turn mode enclosed box body, characterized in that the partition of hole is set in the box body containing two layers, and each layer of partition has thickness Box body is divided into upper layer and lower layer by degree, two layers of partition, wherein in lower layer's partition tool there are three macropore, and upper layer partition have it is multiple Aperture, the lower layer separate two two centers of circle separated in multiple apertures with upper layer in three macropores and hang down respectively same In straight axis.

2. multicolor fluorescence immunohistochemical kit according to claim 1, characterized in that two of above-mentioned lower layer's partition are greatly Two reagent bottles are placed in hole, up-narrow and down-wide platform columnar structures are presented in two reagent bottles, and bottle cap is from described in the partition of upper layer Expose in coaxial aperture, so that upper layer partition be made to be shelved in lower layer's partition by what two reagent bottles consolidated.

3. a kind of multicolor fluorescence immunohistochemical kit according to claim 1 or 2, characterized in that the box body and box Lid is black non transparent material.

4. multicolor fluorescence immunohistochemical kit according to claim 1 or 2, characterized in that two of lower layer's partition are big Two antiantibodys and confining liquid reagent are placed in hole respectively, and another macropore places signal and amplifies liquid reagent.

5. multicolor fluorescence immunohistochemical kit according to claim 1 or 2, characterized in that upper layer partition in addition to Fluorescence is placed respectively with other apertures of the macropore center of circle outside two apertures on same vertical axis described in claim 1 The low capacities reagents such as dyestuff, an antiantibody, mountant.

6. multicolor fluorescence immunohistochemical kit according to claim 1 or 2, characterized in that the box body and box cover are Black non transparent material.