A kind of digital microcurrent-controlled chip report control platform
Technical field
The utility model relates to micro fluidic chip technical field more particularly to a kind of digital microcurrent-controlled chip report control platforms.
Background technique
Existing digital microcurrent-controlled chip generally uses microelectrode drive mode, and microelectrode process is related to sputtering, light
The kinds of processes such as quarter, corrosion and super thin plated film, it is expensive, it is complicated for operation;And microelectrode is entirely disposable, once chip
It is with high costs to there is damaged or parafunction, the chip rejection.
Digital microcurrent-controlled chip main application fields be microlayer model (5-200 μm of diameter) formation and manipulation, in order to it is micro-
The size of drop matches, and a large amount of microlayer model of control is moved, and needs the huge microelectrode of processing quantity, and each micro-
Electrode requires to connect by conducting wire with power supply.When microlayer model quantity is larger, the task that microelectrode is connected with power supply is just
It is abnormal heavy;And traditional microelectrode and chip manufacture is integrated, the material of chip can only select simultaneous with microelectrode processing technology
The material of appearance, higher cost, processing difficulties.
Therefore, a kind of digital microcurrent-controlled chip report control platform is needed, to solve digital microcurrent-controlled chip in the prior art
To the Dependence Problem of microelectrode.
Utility model content
The purpose of this utility model is to provide a kind of digital microcurrent-controlled chip report control platforms, solve digital microcurrent-controlled core
Dependence of the piece to microelectrode realizes the accurate control to microlayer model movement and the fusion and division of microlayer model.
For this purpose, the utility model uses following technical scheme:
A kind of digital microcurrent-controlled chip report control platform, including chip and magnetic control module, the chip and the magnetic control module
Face is arranged, and the microchannel for microlayer model flowing is provided on the chip, and the microlayer model has magnetism, the magnetic control module
Magnetic field strength it is adjustable, the magnetic control module can to the movement of the microlayer model carry out magnetic control.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, the chip includes under board-on-chip and chip
Plate, the board-on-chip and chip lower plate face setting, between the board-on-chip and the chip lower plate described in formation
Microchannel.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, the magnetic control module includes group of magnets and driving
Device, for the group of magnets close to the chip-side, the group of magnets includes several magnet units, and the driver includes several drives
Moving cell, the magnet unit and the driving unit correspond, and the driving unit can regulate and control the magnet unit
Magnetic field strength, the magnet unit can control the movement of the microlayer model.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, the magnet unit is permanent magnet, the drive
Moving cell is mechanical jack, and the mechanical jack can control the distance between the permanent magnet and the chip, is applied with changing
The magnetic field strength being added on the microlayer model.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, the magnet unit is electromagnetic coil, described
Driving unit is electrical component, and the electrical component can regulate and control the magnetic field of the electromagnetic coil, is applied to change described micro-
Magnetic field strength on drop.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, the cross-sectional width and phase of the magnet unit
The spacing of the adjacent magnet unit is all larger than the diameter of the microlayer model.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, magnetic bead is added in the microlayer model, it is described
Magnetic bead makes the microlayer model have magnetism.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, the contact angle of the chip and the microlayer model
Greater than 70 °.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, the chip does not have magnetism.
As the optimal technical scheme of digital microcurrent-controlled chip report control platform, it is machined with fluid channel on the chip, it is described
Fluid channel can limit the motion profile of the microlayer model.
Compared with prior art, the advantages of the utility model and beneficial effect is:
Digital microcurrent-controlled chip report control platform provided by the utility model, by setting magnetic control module to microchannel on chip
In the movement of microlayer model controlled, replace microelectrode with magnetic control module, electric drive mode, pendulum replaced in a manner of magnetic drive
Dependence of the digital microcurrent-controlled chip to microelectrode has been taken off, the accurate control to microlayer model movement is realized;And chip and magnetic control mould
Block uses split-type design, reduces replacement cost.
Digital microcurrent-controlled chip report control platform provided by the utility model, is accurately controlled small by large-sized magnet unit
The microlayer model of size moves, and reduces process operation difficulty and production cost;By changing the operating mode of magnetic control module, realize
Movement, fusion and the division of microlayer model, to realize complicated biochemical reaction.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for the digital microcurrent-controlled chip report control platform that the utility model embodiment one provides;
Fig. 2 is the top view for the digital microcurrent-controlled chip report control platform that the utility model embodiment one provides;
Fig. 3 is the top view for the group of magnets that the utility model embodiment one provides;
Fig. 4 is that the single permanent magnet that the utility model embodiment one provides manipulates the top view of single microlayer model movement (only
Show in running order permanent magnet);
Fig. 5 is the side view that the single permanent magnet that the utility model embodiment one provides manipulates single microlayer model movement;
Fig. 6 is that multiple permanent magnets that the utility model embodiment one provides manipulate the top view of single microlayer model movement (only
Show in running order permanent magnet);
Fig. 7 is that the number by controlling work permanent magnet that the utility model embodiment one provides manipulates single microlayer model fortune
Dynamic side view;
Fig. 8 be the utility model embodiment one provide by control permanent magnet single microlayer model is manipulated at a distance from chip
The side view of movement;
Fig. 9 is the top view that the single electromagnetic coil that the utility model embodiment two provides manipulates single microlayer model movement
(merely illustrating in running order electromagnetic coil);
Figure 10 is the side view that the single electromagnetic coil that the utility model embodiment two provides manipulates single microlayer model movement;
Figure 11 is the top view that multiple electromagnetic coils that the utility model embodiment two provides manipulate single microlayer model movement
(merely illustrating in running order electromagnetic coil);
Figure 12 is that the number by controlling working coil that the utility model embodiment two provides manipulates single micro- liquid
Drip the side view of movement;
Figure 13 is that the turn on angle by controlling electromagnetic coil that the utility model embodiment two provides manipulates single microlayer model
The side view of movement;
Figure 14 is that the top view when microlayer model that the utility model embodiment three provides merges (is merely illustrated in work shape
The magnet unit of state);
Figure 15 is that the fused top view of microlayer model that the utility model embodiment three provides (is merely illustrated in work shape
The magnet unit of state);
Figure 16 is that the top view when microlayer model that the utility model embodiment four provides divides (is merely illustrated in work shape
The magnet unit of state);
Figure 17 is that the top view after the microlayer model division that the utility model embodiment four provides (is merely illustrated in work shape
The magnet unit of state).
In figure:
1- board-on-chip;2- chip lower plate;3- group of magnets;4- driver;5- microlayer model;
31- magnet unit;41- driving unit.
Specific embodiment
The technical solution of the utility model is further illustrated with embodiment with reference to the accompanying drawing.It is understood that this
Locate described specific embodiment and is used only for explaining the utility model, rather than the restriction to the utility model.It further needs exist for
Illustrate, illustrates only part relevant to the utility model for ease of description, in attached drawing and not all.It for the first time will be photic
Shape-changing material is applied to digital microcurrent-controlled field.
Embodiment one
Present embodiment provides a kind of digital microcurrent-controlled chip report control platform, as shown in Figure 1, including chip and magnetic control mould
Block, chip and magnetic control module face are arranged, and the microchannel flowed for microlayer model 5 is provided on chip, and microlayer model 5 has magnetism,
The magnetic field strength of magnetic control module is adjustable, carries out magnetic control so as to the movement to microlayer model 5.
According to concrete application demand, magnetic control module can be all set in the two sides up and down of chip, with right in chip two sides
Microlayer model 5 applies magnetic field, realizes the more accurate and complicated control of motion profile and biochemical reaction to microlayer model 5.As excellent
It selects, the underface of chip is arranged in the magnetic control module in the present embodiment.
The movement of the microlayer model 5 in microchannel on chip is controlled by the way that magnetic control module is arranged, with magnetic control module generation
For microelectrode, electric drive mode is replaced in a manner of magnetic drive, gets rid of digital microcurrent-controlled chip report control platform to microelectrode
It relies on, realizes the accurate control moved to microlayer model 5;Chip and magnetic control module use split-type design, and chip is according to reality
Demand production, magnetic control module are common apparatus, and the size and function of magnetic control module are fixed, and chip can be replaced arbitrarily, solve
Chip and microelectrode one in the prior art, the higher problem of replacement cost.
Referring to fig. 2, A is the entrance of chip in figure, and B is outlet, entrance, that is, microchannel entrance connection routine of chip
The micro-fluidic chips such as microlayer model generating device, such as cross or T-type, the drop generating devices such as piezo nozzles, pass through conduit
Or microlayer model generating device and chip entrance are attached by interface etc., to guarantee that microlayer model 5 enters digital microcurrent-controlled core
In piece report control platform;The outlet of chip connects the devices such as subsequent analysis detection.
It is added to magnetic bead in 5 forming process of microlayer model, so that microlayer model 5 be made to have magnetism, this operation is this field
Routine operation, details are not described herein again.Magnetic bead material can be cellulose, gelatin, polystyrene, polyacrylic acid (ester) and its copolymerization
Object, polyamide-based or polyaniline etc., bead diameter range are 10nm-10 μm, and magnetic bead, which can according to need, to be surface modified, with
Realize specific function.
After microlayer model 5 with magnetic bead enters chip, fortune can be realized under the effect of the magnetic control of magnetic control module
Dynamic and track accurate control.
More specifically, as shown in Figure 1, chip includes board-on-chip 1 and chip lower plate 2, board-on-chip 1 and chip lower plate 2
Face setting, the space between board-on-chip 1 and chip lower plate 2 form microchannel, and the height of microchannel is 1 μm -1000 μm, when
So, the size of microchannel can be designed according to actual needs.The material of board-on-chip 1 and chip lower plate 2 can be micro-fluidic
The rigid polymers such as the common silicon wafer of chip, glass, PDMS and PMMA, PS, PC, can also be flat for the manipulation of digital microcurrent-controlled chip
Platform commonly uses glass or pcb board, the present embodiment are not particularly limited the material of board-on-chip 1 and chip lower plate 2, but for core
The material characteristic of piece upper plate 1 and chip lower plate 2 has following points requirement.
Firstly, chip obtained and the contact angle of drop phase are larger, in drop mutually added surfactant and magnetic bead
In the case of, the contact angle of microlayer model 5 and chip is greater than 70 °, and the contact angle the big more is conducive to the movement of microlayer model 5.If material
Matter itself and the contact angle of drop phase are smaller, can be handled, be changed by the modes such as spraying modifying agent or plasma irradiation
Property agent is selected according to the characteristic of drop phase, can be dimethyldichlorosilane, trim,ethylchlorosilane, silicon fluoride, Triton
And Teflon etc..Furthermore it is required that the surface that chip and microlayer model 5 contact, the i.e. surface of microchannel, surface roughness compared with
It is small, it generally requires roughness to be less than 10nm, is conducive to the movement of microlayer model 5.
Secondly, chip material itself should not have magnetism, to avoid the interference to magnetic control module control action.
Again, with microlayer model 5 any biochemical reaction does not occur for chip material itself, and microlayer model 5 is not stopped to move.
Fluid channel, fluid channel energy can be more preferably processed on the board-on-chip of chip 1 and chip lower plate 2 according to actual needs
Enough limit the motion profile of microlayer model 5.Fluid channel can be added by means such as photoetching, numerical control, reverse mould, 3D printing, laser or injection moldings
Work.
According to the selection of microlayer model generating device and the selection of chip material, can have between microlayer model 5 or not
With continuous phase liquid, if selecting micro-fluidic chip as microlayer model generating device, there is continuous phase;If selecting piezoelectricity
Nozzle does not have continuous phase then as microlayer model generating device.
Magnetic control module in the present embodiment includes group of magnets 3 and driver 4, and the setting of group of magnets 3 is close above driver 4
The side of chip, group of magnets 3 include several magnet units 31, and driver 4 includes several driving units 41, magnet unit 31 and drive
Moving cell 41 corresponds, and driving unit 41 can regulate and control the magnetic field strength of magnet unit 31, and magnet unit 31 can control micro-
The movement of drop 5.
Referring to Fig. 3, the cross sectional shape of magnet unit 31 is selected according to the size of working ability and driver 4, can be with
It is square or other shapes, in the present embodiment by taking square as an example, W indicates cross-sectional width, and the value range of W is 0.1mm-
The smaller control for being more conducive to microlayer model 5 of 20mm, W value;Spacing G between adjacent magnets unit 31 is according to the ability of driver 4
Setting, the value range of G are 0.05mm-20mm, the smaller control for being more conducive to microlayer model 5 of G value.W value and G in the present embodiment
Value can be much larger than microlayer model 5 diameter, compared to the prior art in, the microelectrode ruler of digital microcurrent-controlled chip report control platform
It is very little necessary suitable with 5 size of microlayer model, difficulty of processing and cost are significantly reduced, there is great advantage.
Preferably, the magnetic control module in the present embodiment, specially driver 4, it can be with external computer, by computer
Whether the work of each driving unit 41 of software control, to realize the manipulation to microlayer model 5;And simulation softward can be passed through
(COMSOL, FLUENT etc.) realizes the calculating of each 41 magnetic field strength of driving unit, moves to further realize to microlayer model 5
More precise control can also greatly improve manipulation speed, so that the utility model is applied to gene sequencing, pharmaceutical synthesis, is resisted
The biochemistries such as body screening and single cell analysis.
Magnet unit 31 in the present embodiment is permanent magnet, and driver 4 is selected according to the material of magnet unit 31, is had
For body, driver 4 is made of a series of mechanical motion devices, such as driving unit 41 is mandril, each mandril corresponding one
A permanent magnet controls its corresponding permanent magnet by mandril and moves upwards in the side perpendicular to chip, leans on to control permanent magnet
It close or remote from chip, and can control the distance between permanent magnet and chip, realize the control of magnetic field power, applied with changing
The intensity in magnetic field on microlayer model 5 promotes microlayer model 5 to move.
The control method of the present embodiment is further illustrated below with reference to specific example.
It should be noted that since the magnetic bead content wrapped up in microlayer model 5 is less, as long as magnet unit 31 is apart from core
Piece has certain distance, and magnet unit 31 can almost ignore the magnetic field of microlayer model 5.
Fig. 4 and Fig. 5 shows single permanent magnet and manipulates the working principle diagram that single microlayer model 5 moves, for convenience, figure
In running order magnet unit 31 (similarly hereinafter) is illustrated only in 4.In order to make microlayer model 5 move to a certain certain bits on chip
It sets, can be promoted a certain magnet unit 31 by driver 4, i.e., the mandril at the position is upward by corresponding permanent magnet
It jacks up, by the permanent magnet close to chip lower plate 2, applies magnetic field to microlayer model 5, the control moved to microlayer model 5 is realized by magnetic force
System, finally makes microlayer model 5 reach designated position.
Fig. 6 and Fig. 7 shows multiple permanent magnets and manipulates the working principle diagram that single microlayer model 5 moves, due to permanent magnet
Size is much larger than 5 size of microlayer model, is located at the excentral position of some permanent magnet if necessary to microlayer model 5, can pass through control
It makes multiple permanent magnets to work at the same time, magnetic field strength can be with partial offset, so that the microlayer model 5 for being enclosed with magnetic bead is fixed on permanent magnetism
Some excentral position of iron.Fig. 7 show side view when five permanent magnets work together, in running order in Fig. 7
Permanent magnet be equal at a distance from chip.
In addition, in addition to the number for controlling work permanent magnet, it can also be real by adjusting each permanent magnet at a distance from chip
The adjusting of existing magnetic field strength, thus realize the control of 5 position of microlayer model, it is specific as shown in Figure 8.
In addition, when multiple permanent magnets work at the same time, by software (COMSOL, FLUENT etc.) to the magnetic field strength of permanent magnet
It is calculated, to realize magnetic field strength of the adjustment effect on microlayer model 5 by the magnetic field strength for regulating and controlling each permanent magnet
It adjusts, and then realizes the adjusting of microlayer model 5 position and movement velocity.
Embodiment two
The present embodiment provides a kind of digital microcurrent-controlled chip report control platform, the present embodiment is basically the same as the first embodiment, and is
For the sake of simplicity, the difference of the present embodiment Yu embodiment one is only described.
The magnet unit 31 of group of magnets 3 is electromagnetic coil in the present embodiment, and the driving unit 41 of driver 4 is electrical member
Part whether energization by controlling electrical component, has magnetic or does not have magnetism, and can lead to control electromagnetic coil
It crosses control current strength and realizes the adjusting of magnetic field strength, to change the magnetic field strength being applied on microlayer model 5, control microlayer model
5 are moved.
The control method of the present embodiment is further illustrated below with reference to specific example.
Fig. 9 and Figure 10 shows single electromagnetic coil and manipulates the working principle diagram that single microlayer model 5 moves, as seen from the figure,
It can be by the movement and position control for realizing microlayer model 5 whether energization of control electromagnetic coil, by controlling specific location
Electrical component give its corresponding solenoid, so that it may so that it generates magnetic, realize the control of microlayer model 5.
Figure 11 and Figure 12 shows multiple electromagnetic coils and manipulates the working principle diagram that single microlayer model 5 moves, due to electromagnetism
The size of coil is much larger than 5 size of microlayer model, if necessary to by the control of microlayer model 5 other than electromagnetic coil center, can be with
It is worked at the same time by controlling multiple electromagnetic coils, to control magnetic field strength, realizes the accurate control of 5 position of microlayer model.
In addition, can also be controlled not by software (COMSOL, FLUENT etc.) in addition to the number for controlling working coil
Magnetic field strength is controlled with the turn on angle of electromagnetic coil, and then realizes the accurate adjusting of 5 position of microlayer model and movement velocity, specifically
As shown in figure 13, hatching different in figure indicates that turn on angle is different, so that the magnetism of each electromagnetic coil is different.
Embodiment three
The method that microlayer model 5 merges is carried out using embodiment one and embodiment two the present embodiment provides a kind of.
It usually needs to merge two kinds of reactants in biochemical reaction, be reacted after applying certain condition, the utility model
This function can be well realized in the digital microcurrent-controlled chip report control platform provided, by that will contain the micro- of different chemical substances
Drop 5 merges, and realizes the purpose of reaction.
Specifically, as shown in Figure 14 and Figure 15, Figure 14 is that single magnet unit 31 controls two microlayer models 5 to a certain spy
Movement is set in positioning, and after two microlayer models 5 reach the position, collide fusion, and fusion becomes a biggish microlayer model 5
Afterwards, as shown in figure 15, it is eventually located at the center of the magnet unit 31.As described in embodiment one and embodiment two, Ke Yitong
Permanent magnet or solenoid controlled microlayer model 5 are crossed, can also be controlled by the magnetic field strength of multiple permanent magnets or electromagnetic coil
The movement of microlayer model 5 and position.
Example IV
The method that microlayer model 5 divides is carried out using embodiment one and embodiment two the present embodiment provides a kind of.
The operation that diluent materials are usually needed in biochemical reaction usually passes through in digital microcurrent-controlled chip report control platform
First dilution and microlayer model 5 are merged, then biggish microlayer model 5 is split into lesser microlayer model 5, to realize target substance
Dilution, this process is often than relatively time-consuming, and method provided in this embodiment can fast implement biggish microlayer model 5 and split into
The purpose of lesser microlayer model 5.
Specifically, as shown in Figure 16 and Figure 17, Figure 16 is that two magnet units 31 act on a biggish microlayer model 5
On, two magnet units 31 apply the power of two opposite directions to biggish microlayer model 5, biggish microlayer model 5 can be divided
At two lesser microlayer models 5, diluted unit operation is realized.It, can be by forever as described in embodiment one and embodiment two
Magnet or solenoid controlled microlayer model 5 can also control micro- liquid by the magnetic field strength of multiple permanent magnets or electromagnetic coil
The movement of drop 5 and position.
Digital microcurrent-controlled chip report control platform provided by the utility model, it is real by changing the operating mode of magnetic control module
Movement, fusion and the division of existing microlayer model 5, to realize complicated biochemical reaction, the external computer of magnetic control module passes through software reality
It now calculates and controls, more precise control is moved to microlayer model 5 to realize, can also greatly improve manipulation speed, to incite somebody to action this
Utility model is applied to the biochemistries such as gene sequencing, pharmaceutical synthesis, antibody screening and single cell analysis.
Obviously, the above embodiments of the present invention is used for the purpose of clearly illustrating examples for clearly illustrating the present invention, and
It is not limitations of the embodiments of the present invention.For those of ordinary skill in the art, in above description
On the basis of can also make other variations or changes in different ways.There is no need and unable to give all embodiments
Exhaustion.Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention etc., should be included in
Within the protection scope of the utility model claims.