CN208621625U - A kind of leukemia drug susceptibility detection kit - Google Patents
A kind of leukemia drug susceptibility detection kit Download PDFInfo
- Publication number
- CN208621625U CN208621625U CN201821304480.0U CN201821304480U CN208621625U CN 208621625 U CN208621625 U CN 208621625U CN 201821304480 U CN201821304480 U CN 201821304480U CN 208621625 U CN208621625 U CN 208621625U
- Authority
- CN
- China
- Prior art keywords
- pull rod
- chamber
- wall
- sealing plate
- drug susceptibility
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of leukemia drug susceptibility detection kits, including reagent box body, box cover is threaded on the bottleneck of the reagent box body, the upper end of the box cover is equipped with feed inlet, device chamber is equipped in the box cover, described device is intracavitary to be equipped with pulling mechanism, the pulling mechanism is connected with sealing plate, the sealing plate runs through the inner wall of device chamber and extends in feed inlet, annular chamber is equipped in the side wall of the reagent box body, black black-out cloth is covered in the annular chamber, the lower end of the black black-out cloth is fixedly connected with circular rod, the side wall of the circular rod is fixedly connected with the first pull rod, the outside inner wall of the annular chamber is equipped with the first strip-shaped hole, first pull rod runs through the first strip-shaped hole.The utility model is structurally reasonable, can open lesser opening so that dropper instills solution, avoid excessive bacterium from entering in kit, and light-proofness is good, nor affect on observation.
Description
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of leukemia drug susceptibility detection kits.
Background technique
Leukemia Drug Sensitivity detection at present often uses mtt assay, and mtt assay is also known as MTT colorimetric method, is that a kind of detection cell is deposited
Living and growth method.Its testing principle is that the succinate dehydrogenase in living cells mitochondria can make exogenous MTT be reduced to water
Insoluble bluish violet crystallizes first a ceremonial jade-ladle, used in libation and is deposited in cell, and dead cell is without this function.Dimethyl sulfoxide can dissolve in cell
First a ceremonial jade-ladle, used in libation, measure its absorbance value at 540 or 720nm wavelength with enzyme-linked immunosorbent assay instrument, can reflect living cells quantity indirectly.
Within the scope of certain cell number, it is directly proportional to cell number that MTT crystallizes the amount to be formed.This method is widely used in some bioactivity
The Activity determination of the factor, large-scale screening anti-tumor medicine, cell toxicity test and tumor radiosensitivity measurement etc..
Now when detecting, solution is typically injected progress hybrid reaction in kit to detect, MTT solution reaction
When not visible light, and existing kit light-proofness is poor, and MTT solution is typically all to be pressed into reagent from bottleneck using dropper
In box, but the bottleneck of existing kit is larger, and when opening is easily accessible more bacterium, influences testing result.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of leukemia drug susceptibility proposed
Detection kit can open lesser opening so that dropper instills solution, excessive bacterium is avoided to enter in kit, and
And light-proofness is good, nor affects on observation.
To achieve the goals above, present invention employs following technical solutions:
A kind of leukemia drug susceptibility detection kit, including reagent box body, spiral shell on the bottleneck of the reagent box body
Line is connected with box cover, and the upper end of the box cover is equipped with feed inlet, and device chamber is equipped in the box cover, and intracavitary be equipped with of described device is drawn
Motivation structure, the pulling mechanism are connected with sealing plate, and the sealing plate runs through the inner wall of device chamber and extends in feed inlet, institute
It states and is equipped with annular chamber in the side wall of reagent box body, be covered with black black-out cloth in the annular chamber, the black black-out cloth
Lower end is fixedly connected with circular rod, and the side wall of the circular rod is fixedly connected with the first pull rod, the outside inner wall of the annular chamber
Equipped with the first strip-shaped hole, first pull rod runs through the first strip-shaped hole, passes through between the circular rod and the interior bottom of annular chamber
Fixed mechanism connection.
Preferably, the lower end of the sealing plate is equipped with gasket.
Preferably, the feed inlet is equipped with slot far from the side of device chamber, the end of the sealing plate and slot it is interior
Wall offsets.
Preferably, the pulling mechanism include positioned at intracavitary the second pull rod of device, the side of second pull rod with it is close
The side wall of sealing plate is fixedly connected, and described device lumenal levels are fixedly connected with guide rod, and the guide rod is through the second pull rod
Side wall is arranged with spring on the guide rod, and the spring is located between the other side of the second pull rod and the inner wall of device chamber, institute
The inner top for stating device chamber is equipped with the second strip-shaped hole, and the second strip-shaped hole is run through in the upper end of second pull rod.
Preferably, the annular chamber is in inverted L-shaped.
Preferably, the fixed mechanism includes annular iron plate, and the annular iron plate is fixed on the interior bottom of annular chamber, described
The lower end of circular rod is equidistantly embedded with multiple magnet blocks.
Compared with prior art, the present invention has the advantages that:
1, it is provided with feed inlet, sealing plate and pulling mechanism, sealing plate is opened according to the needs of use and injects solution, be not necessarily to
It opens the lid, a large amount of bacterium is avoided to enter.
2, it is provided with black black-out cloth and the first pull rod, black black-out cloth can be put down in reaction, avoid light pair
The influence of reaction, and it is provided with fixed mechanism, observing response situation can be pulled at any time.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of leukemia drug susceptibility detection kit proposed by the present invention；
Fig. 2 is structure enlargement diagram at a kind of A of leukemia drug susceptibility detection kit proposed by the present invention；
Fig. 3 is structure enlargement diagram at a kind of B of leukemia drug susceptibility detection kit proposed by the present invention；
Fig. 4 is schematic diagram at a kind of C-C of leukemia drug susceptibility detection kit proposed by the present invention.
In figure: 1 box cover, 2 sealing plates, 3 feed inlets, 4 black black-out clothes, 5 reagent box bodies, 6 annular chambers, 7 second pull rods,
8 guide rods, 9 springs, 10 device chambers, 11 circular rods, 12 magnet blocks, 13 annular iron plates, 14 first pull rods.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Referring to Fig.1-4, a kind of leukemia drug susceptibility detection kit, including reagent box body 5, reagent box body 5
Box cover 1 is threaded on bottleneck, the upper end of box cover 1 is equipped with feed inlet 3, device chamber 10 is equipped in box cover 1, is set in device chamber 10
There is pulling mechanism, pulling mechanism is connected with sealing plate 2, and pulling mechanism includes the second pull rod 7 in device chamber 10, and second draws
The side of bar 7 is fixedly connected with the side wall of sealing plate 2, and level is fixedly connected with guide rod 8 in device chamber 10, and guide rod 8 runs through
The side wall of second pull rod 7, is arranged with spring 9 on guide rod 8, and spring 9 is located at the interior of the other side of the second pull rod 7 and device chamber 10
Between wall, the inner top of device chamber 10 is equipped with the second strip-shaped hole, and the second strip-shaped hole is run through in the upper end of the second pull rod 7, and sealing plate 2 passes through
The inner wall of penetrating device chamber 10 simultaneously extends in feed inlet 3, and annular chamber 6 is equipped in the side wall of reagent box body 5, is covered in annular chamber 6
It is stamped black black-out cloth 4, the lower end of black black-out cloth 4 is fixedly connected with circular rod 11, and the side wall of circular rod 11 is fixedly connected with
First pull rod 14, the outside inner wall of annular chamber 6 are equipped with the first strip-shaped hole, and the first pull rod 14 runs through the first strip-shaped hole, circular rod 11
It is connect between the interior bottom of annular chamber 6 by fixed mechanism, fixed mechanism includes annular iron plate 13, and annular iron plate 13 is fixed on
The interior bottom of annular chamber 6, the lower end of circular rod 11 are equidistantly embedded with multiple magnet blocks 12, when injecting solution, need to only draw to the right
Dynamic second pull rod 7, sealing plate 2 is drawn in device chamber 10 along guide rod 8, is instilled solution from feed inlet 3 using dropper and is tried
In agent box body 5,1 injected without directly opening the lid, so that excessive bacterium is avoided to enter in reagent box body 5, shadow
Testing result is rung, when reaction, black black-out cloth 4 is pulled down, light is avoided to irradiate into reagent box body 5,11 lower end of circular rod
Magnet block 12 connect with annular iron plate magnetism and is fixed, and can pull the first pull rod 14 directly up, opening black black-out cloth 4 into
Row observation.
In the present invention, the lower end of sealing plate 2 is equipped with gasket, and feed inlet 3 is equipped with slot far from the side of device chamber 10,
The end of sealing plate 2 and the inner wall of slot offset, and increase leakproofness, air is avoided to enter.Annular chamber 6 is in inverted L-shaped, makes kit
The side wall of 5 top of ontology also obtains the covering of black black-out cloth 4, and light-proofness is more preferable.
In the present invention, when injecting solution, the second pull rod 7 only need to be pulled to the right, sealing plate 2 is drawn in into dress along guide rod 8
It sets in chamber 10, solution is instilled in reagent box body 5 from feed inlet 3 using dropper, 1 is injected without directly opening the lid,
To avoiding excessive bacterium from entering in reagent box body 5, testing result is influenced, when reaction, pull down black black-out cloth 4, avoid
Light is irradiated into reagent box body 5, and the magnet block 12 of 11 lower end of circular rod connect with annular iron plate magnetism and is fixed, can be straight
It connects and pulls up the first pull rod 14, open black black-out cloth 4 and observed.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of leukemia drug susceptibility detection kit, including reagent box body (5), which is characterized in that the kit sheet
It is threaded on the bottleneck of body (5) box cover (1), the upper end of the box cover (1) is equipped with feed inlet (3), sets in the box cover (1)
Have device chamber (10), pulling mechanism is equipped in described device chamber (10), the pulling mechanism is connected with sealing plate (2), described close
Sealing plate (2) runs through the inner wall of device chamber (10) and extends in feed inlet (3), is equipped in the side wall of the reagent box body (5)
Annular chamber (6), the annular chamber (6) is interior to be covered with black black-out cloth (4), and the lower end of the black black-out cloth (4) is fixedly connected
Have circular rod (11), the side wall of the circular rod (11) is fixedly connected with the first pull rod (14), in the outside of the annular chamber (6)
Wall is equipped with the first strip-shaped hole, and first pull rod (14) runs through the first strip-shaped hole, and the circular rod (11) is interior with annular chamber (6)
It is connected between bottom by fixed mechanism.
2. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the sealing plate
(2) lower end is equipped with gasket.
3. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the feed inlet
(3) side far from device chamber (10) is equipped with slot, and the end of the sealing plate (2) and the inner wall of slot offset.
4. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the pulling mechanism
Including the second pull rod (7) being located in device chamber (10), the side of second pull rod (7) and the side wall of sealing plate (2) are fixed
Connection, described device chamber (10) interior level are fixedly connected with guide rod (8), and the guide rod (8) runs through the side of the second pull rod (7)
Wall is arranged with spring (9) on the guide rod (8), and the spring (9) is located at the other side and the device chamber of the second pull rod (7)
(10) between inner wall, the inner top of described device chamber (10) is equipped with the second strip-shaped hole, and the upper end of second pull rod (7) is run through
Second strip-shaped hole.
5. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the annular chamber
It (6) is in inverted L-shaped.
6. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the fixed mechanism
Including annular iron plate (13), the annular iron plate (13) is fixed on the interior bottom of annular chamber (6), the lower end of the circular rod (11)
Equidistantly it is embedded with multiple magnet blocks (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821304480.0U CN208621625U (en) | 2018-08-14 | 2018-08-14 | A kind of leukemia drug susceptibility detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821304480.0U CN208621625U (en) | 2018-08-14 | 2018-08-14 | A kind of leukemia drug susceptibility detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208621625U true CN208621625U (en) | 2019-03-19 |
Family
ID=65716627
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821304480.0U Expired - Fee Related CN208621625U (en) | 2018-08-14 | 2018-08-14 | A kind of leukemia drug susceptibility detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208621625U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108828218A (en) * | 2018-08-14 | 2018-11-16 | 南京融康博生物科技有限公司 | A kind of leukemia drug susceptibility detection kit |
-
2018
- 2018-08-14 CN CN201821304480.0U patent/CN208621625U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108828218A (en) * | 2018-08-14 | 2018-11-16 | 南京融康博生物科技有限公司 | A kind of leukemia drug susceptibility detection kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105153214B (en) | A kind of silicon substrate rhodamine nitric oxide fluorescent probe and its preparation method and application | |
| Yang et al. | A near-infrared fluorescent probe based on phosphorus-substituted rhodamine for deep imaging of endogenous hypochlorous acid in vivo | |
| CN108398409B (en) | Method for detecting hypochlorite by fluorescence ratio | |
| CN208621625U (en) | A kind of leukemia drug susceptibility detection kit | |
| CN105693703A (en) | Novel rate type fluorescent probe for intracellular lysosome pH imaging | |
| CN101393190A (en) | Cell toxicity determination method in cigarette mainstream flue gas | |
| Joo et al. | A dicyanocoumarin-fused quinolinium based probe for NAD (P) H and its use for detecting glycolysis and hypoxia in living cells and tumor spheroids | |
| CN109112105A (en) | A kind of method for building up and verification method of human hepatoma cell line HepG2's oxidative stress model of oleic acid induction | |
| CN205120592U (en) | Little biological activity detector of agricultural product | |
| CN108828218A (en) | A kind of leukemia drug susceptibility detection kit | |
| CN109797192A (en) | A kind of fast quantification senile cell detection method based on senescence associated-β-galactosidase | |
| CN104293884B (en) | A kind of acetyl CoA contents measures test kit and method thereof | |
| CN110274800A (en) | A kind of soil incubation device and soil incubation and gas production method based on the device | |
| CN101620188A (en) | Method for quickly measuring total number of live bacteria of luminous bacteria by using MTT method | |
| CN110487779A (en) | A kind of kit and its detection method of chemoluminescence method quantitative detection trichomonas vaginalis | |
| CN109776399A (en) | Fluorescent probe compounds and preparation method and its endotoxic application of detection and method | |
| CN109142224A (en) | A kind of intelligent portable ammonia nitrogen detector | |
| CN101724682B (en) | Staining fluid and method for rapidly detecting novel cryptococcus | |
| CN205263083U (en) | Experimental device for under responding, indoor simulation alternation of wetting and drying surveys soil respiration | |
| CN207992230U (en) | A kind of food inspection kit for detecting organophosphor | |
| CN209460124U (en) | A kind of intelligent portable ammonia nitrogen detector | |
| CN206990616U (en) | A kind of medical multifunctional in-vitro diagnosis Medical Devices | |
| CN208171956U (en) | Calibrating installation for gas detecting instrument calibrating | |
| CN208420892U (en) | A kind of environment measuring water quality detecting device | |
| CN207423973U (en) | A kind of anti-pollution test card |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190319 Termination date: 20210814 |