CN108828218A - A kind of leukemia drug susceptibility detection kit - Google Patents
A kind of leukemia drug susceptibility detection kit Download PDFInfo
- Publication number
- CN108828218A CN108828218A CN201810921159.5A CN201810921159A CN108828218A CN 108828218 A CN108828218 A CN 108828218A CN 201810921159 A CN201810921159 A CN 201810921159A CN 108828218 A CN108828218 A CN 108828218A
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- China
- Prior art keywords
- pull rod
- chamber
- sealing plate
- drug susceptibility
- detection kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000001514 detection method Methods 0.000 title claims abstract description 19
- 239000003814 drug Substances 0.000 title claims abstract description 19
- 229940079593 drug Drugs 0.000 title claims abstract description 18
- 208000032839 leukemia Diseases 0.000 title claims abstract description 18
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 17
- 208000003443 Unconsciousness Diseases 0.000 claims abstract description 16
- 239000004744 fabric Substances 0.000 claims abstract description 15
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 18
- 229910052742 iron Inorganic materials 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000005389 magnetism Effects 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 1
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000007541 cellular toxicity Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57426—Specifically defined cancers leukemia
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of leukemia drug susceptibility detection kits, including reagent box body, box cover is threaded on the bottleneck of the reagent box body, the upper end of the box cover is equipped with feed inlet, device chamber is equipped in the box cover, described device is intracavitary to be equipped with pulling mechanism, the pulling mechanism is connected with sealing plate, the sealing plate runs through the inner wall of device chamber and extends in feed inlet, annular chamber is equipped in the side wall of the reagent box body, black black-out cloth is covered in the annular chamber, the lower end of the black black-out cloth is fixedly connected with circular rod, the side wall of the circular rod is fixedly connected with the first pull rod, the outside inner wall of the annular chamber is equipped with the first strip-shaped hole, first pull rod runs through the first strip-shaped hole.Structure of the invention is reasonable, can open lesser opening so that dropper instills solution, avoid excessive bacterium from entering in kit, and light-proofness is good, nor affect on observation.
Description
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of leukemia drug susceptibility detection kits.
Background technique
Leukemia Drug Sensitivity detection at present often uses mtt assay, and mtt assay is also known as MTT colorimetric method, is that a kind of detection cell is deposited
Living and growth method.Its testing principle is that the succinate dehydrogenase in living cells mitochondria can make exogenous MTT be reduced to water
Insoluble bluish violet crystallizes first a ceremonial jade-ladle, used in libation and is deposited in cell, and dead cell is without this function.Dimethyl sulfoxide can dissolve in cell
First a ceremonial jade-ladle, used in libation, measure its absorbance value at 540 or 720nm wavelength with enzyme-linked immunosorbent assay instrument, can reflect living cells quantity indirectly.
Within the scope of certain cell number, it is directly proportional to cell number that MTT crystallizes the amount to be formed.This method is widely used in some bioactivity
The Activity determination of the factor, large-scale screening anti-tumor medicine, cell toxicity test and tumor radiosensitivity measurement etc..
Now when detecting, solution is typically injected progress hybrid reaction in kit to detect, MTT solution reaction
When not visible light, and existing kit light-proofness is poor, and MTT solution is typically all to be pressed into reagent from bottleneck using dropper
In box, but the bottleneck of existing kit is larger, and when opening is easily accessible more bacterium, influences testing result.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of leukemia drug susceptibility proposed
Detection kit can open lesser opening so that dropper instills solution, excessive bacterium is avoided to enter in kit, and
And light-proofness is good, nor affects on observation.
To achieve the goals above, present invention employs following technical solutions：
A kind of leukemia drug susceptibility detection kit, including reagent box body, screw thread connects on the bottleneck of the reagent box body
It is connected to box cover, the upper end of the box cover is equipped with feed inlet, and device chamber is equipped in the box cover, and described device is intracavitary to be equipped with pulling machine
Structure, the pulling mechanism are connected with sealing plate, and the sealing plate runs through the inner wall of device chamber and extends in feed inlet, the examination
It is equipped with annular chamber in the side wall of agent box body, is covered with black black-out cloth, the lower end of the black black-out cloth in the annular chamber
It is fixedly connected with circular rod, the side wall of the circular rod is fixedly connected with the first pull rod, and the outside inner wall of the annular chamber is equipped with
First strip-shaped hole, first pull rod run through the first strip-shaped hole, pass through fixation between the circular rod and the interior bottom of annular chamber
Mechanism connection.
Preferably, the lower end of the sealing plate is equipped with gasket.
Preferably, the feed inlet is equipped with slot far from the side of device chamber, the end of the sealing plate and slot it is interior
Wall offsets.
Preferably, the pulling mechanism include positioned at intracavitary the second pull rod of device, the side of second pull rod with it is close
The side wall of sealing plate is fixedly connected, and described device lumenal levels are fixedly connected with guide rod, and the guide rod is through the second pull rod
Side wall is arranged with spring on the guide rod, and the spring is located between the other side of the second pull rod and the inner wall of device chamber, institute
The inner top for stating device chamber is equipped with the second strip-shaped hole, and the second strip-shaped hole is run through in the upper end of second pull rod.
Preferably, the annular chamber is in inverted L-shaped.
Preferably, the fixed mechanism includes annular iron plate, and the annular iron plate is fixed on the interior bottom of annular chamber, described
The lower end of circular rod is equidistantly embedded with multiple magnet blocks.
Compared with prior art, the present invention its advantages are：
1, it is provided with feed inlet, sealing plate and pulling mechanism, sealing plate is opened according to the needs of use and injects solution, without opening
Box cover avoids a large amount of bacterium from entering.
2, it is provided with black black-out cloth and the first pull rod, black black-out cloth can be put down in reaction, avoid light pair
The influence of reaction, and it is provided with fixed mechanism, observing response situation can be pulled at any time.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of leukemia drug susceptibility detection kit proposed by the present invention；
Fig. 2 is structure enlargement diagram at a kind of A of leukemia drug susceptibility detection kit proposed by the present invention；
Fig. 3 is structure enlargement diagram at a kind of B of leukemia drug susceptibility detection kit proposed by the present invention；
Fig. 4 is schematic diagram at a kind of C-C of leukemia drug susceptibility detection kit proposed by the present invention.
In figure：1 box cover, 2 sealing plates, 3 feed inlets, 4 black black-out clothes, 5 reagent box bodies, 6 annular chambers, 7 second pull rods,
8 guide rods, 9 springs, 10 device chambers, 11 circular rods, 12 magnet blocks, 13 annular iron plates, 14 first pull rods.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Referring to Fig.1-4, a kind of leukemia drug susceptibility detection kit, including reagent box body 5, reagent box body 5
Box cover 1 is threaded on bottleneck, the upper end of box cover 1 is equipped with feed inlet 3, device chamber 10 is equipped in box cover 1, is set in device chamber 10
There is pulling mechanism, pulling mechanism is connected with sealing plate 2, and pulling mechanism includes the second pull rod 7 in device chamber 10, and second draws
The side of bar 7 is fixedly connected with the side wall of sealing plate 2, and level is fixedly connected with guide rod 8 in device chamber 10, and guide rod 8 runs through
The side wall of second pull rod 7, is arranged with spring 9 on guide rod 8, and spring 9 is located at the interior of the other side of the second pull rod 7 and device chamber 10
Between wall, the inner top of device chamber 10 is equipped with the second strip-shaped hole, and the second strip-shaped hole is run through in the upper end of the second pull rod 7, and sealing plate 2 passes through
The inner wall of penetrating device chamber 10 simultaneously extends in feed inlet 3, and annular chamber 6 is equipped in the side wall of reagent box body 5, is covered in annular chamber 6
It is stamped black black-out cloth 4, the lower end of black black-out cloth 4 is fixedly connected with circular rod 11, and the side wall of circular rod 11 is fixedly connected with
First pull rod 14, the outside inner wall of annular chamber 6 are equipped with the first strip-shaped hole, and the first pull rod 14 runs through the first strip-shaped hole, circular rod 11
It is connect between the interior bottom of annular chamber 6 by fixed mechanism, fixed mechanism includes annular iron plate 13, and annular iron plate 13 is fixed on
The interior bottom of annular chamber 6, the lower end of circular rod 11 are equidistantly embedded with multiple magnet blocks 12, when injecting solution, need to only draw to the right
Dynamic second pull rod 7, sealing plate 2 is drawn in device chamber 10 along guide rod 8, is instilled solution from feed inlet 3 using dropper and is tried
In agent box body 5,1 injected without directly opening the lid, so that excessive bacterium is avoided to enter in reagent box body 5, shadow
Testing result is rung, when reaction, black black-out cloth 4 is pulled down, light is avoided to irradiate into reagent box body 5,11 lower end of circular rod
Magnet block 12 connect with annular iron plate magnetism and is fixed, and can pull the first pull rod 14 directly up, opening black black-out cloth 4 into
Row observation.
In the present invention, the lower end of sealing plate 2 is equipped with gasket, and feed inlet 3 is equipped with slot far from the side of device chamber 10,
The end of sealing plate 2 and the inner wall of slot offset, and increase leakproofness, air is avoided to enter.Annular chamber 6 is in inverted L-shaped, makes kit
The side wall of 5 top of ontology also obtains the covering of black black-out cloth 4, and light-proofness is more preferable.
In the present invention, when injecting solution, the second pull rod 7 only need to be pulled to the right, sealing plate 2 is drawn in into dress along guide rod 8
It sets in chamber 10, solution is instilled in reagent box body 5 from feed inlet 3 using dropper, 1 is injected without directly opening the lid,
To avoiding excessive bacterium from entering in reagent box body 5, testing result is influenced, when reaction, pull down black black-out cloth 4, avoid
Light is irradiated into reagent box body 5, and the magnet block 12 of 11 lower end of circular rod connect with annular iron plate magnetism and is fixed, can be straight
It connects and pulls up the first pull rod 14, open black black-out cloth 4 and observed.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of leukemia drug susceptibility detection kit, including reagent box body（5）, which is characterized in that the kit sheet
Body（5）Bottleneck on be threaded with box cover（1）, the box cover（1）Upper end be equipped with feed inlet（3）, the box cover（1）Inside set
There is device chamber（10）, described device chamber（10）Interior to be equipped with pulling mechanism, the pulling mechanism is connected with sealing plate（2）, described close
Sealing plate（2）Through device chamber（10）Inner wall and extend to feed inlet（3）It is interior, the reagent box body（5）Side wall in be equipped with
Annular chamber（6）, the annular chamber（6）Inside it is covered with black black-out cloth（4）, the black black-out cloth（4）Lower end be fixedly connected
There is circular rod（11）, the circular rod（11）Side wall be fixedly connected with the first pull rod（14）, the annular chamber（6）Outside in
Wall is equipped with the first strip-shaped hole, first pull rod（14）Through the first strip-shaped hole, the circular rod（11）With annular chamber（6）It is interior
It is connected between bottom by fixed mechanism.
2. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the sealing plate
（2）Lower end be equipped with gasket.
3. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the feed inlet
（3）Far from device chamber（10）Side be equipped with slot, the sealing plate（2）End and the inner wall of slot offset.
4. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the pulling mechanism
Including being located at device chamber（10）The second interior pull rod（7）, second pull rod（7）Side and sealing plate（2）Side wall fix
Connection, described device chamber（10）Interior level is fixedly connected with guide rod（8）, the guide rod（8）Through the second pull rod（7）Side
Wall, the guide rod（8）On be arranged with spring（9）, the spring（9）Positioned at the second pull rod（7）The other side and device chamber
（10）Inner wall between, described device chamber（10）Inner top be equipped with the second strip-shaped hole, second pull rod（7）Upper end run through
Second strip-shaped hole.
5. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the annular chamber
（6）In inverted L-shaped.
6. a kind of leukemia drug susceptibility detection kit according to claim 1, which is characterized in that the fixed mechanism
Including annular iron plate（13）, the annular iron plate（13）It is fixed on annular chamber（6）Interior bottom, the circular rod（11）Lower end
Equidistantly it is embedded with multiple magnet blocks（12）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810921159.5A CN108828218A (en) | 2018-08-14 | 2018-08-14 | A kind of leukemia drug susceptibility detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810921159.5A CN108828218A (en) | 2018-08-14 | 2018-08-14 | A kind of leukemia drug susceptibility detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108828218A true CN108828218A (en) | 2018-11-16 |
Family
ID=64152987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810921159.5A Pending CN108828218A (en) | 2018-08-14 | 2018-08-14 | A kind of leukemia drug susceptibility detection kit |
Country Status (1)
| Country | Link |
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| CN (1) | CN108828218A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203780918U (en) * | 2014-04-03 | 2014-08-20 | 普生（天津）科技有限公司 | Reagent bottle capable of conveniently detecting reagent quality with good photopathy |
| CN204368666U (en) * | 2014-12-30 | 2015-06-03 | 四川大学华西医院 | Volatile medicine bottle cap |
| CN207071424U (en) * | 2017-08-22 | 2018-03-06 | 安徽瑞思威尔科技有限公司 | A kind of Multifunctional test tube rack |
| CN108120629A (en) * | 2018-03-08 | 2018-06-05 | 合肥职业技术学院 | One kind can be protected from light staining jar |
| CN208621625U (en) * | 2018-08-14 | 2019-03-19 | 南京融康博生物科技有限公司 | A kind of leukemia drug susceptibility detection kit |
-
2018
- 2018-08-14 CN CN201810921159.5A patent/CN108828218A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203780918U (en) * | 2014-04-03 | 2014-08-20 | 普生（天津）科技有限公司 | Reagent bottle capable of conveniently detecting reagent quality with good photopathy |
| CN204368666U (en) * | 2014-12-30 | 2015-06-03 | 四川大学华西医院 | Volatile medicine bottle cap |
| CN207071424U (en) * | 2017-08-22 | 2018-03-06 | 安徽瑞思威尔科技有限公司 | A kind of Multifunctional test tube rack |
| CN108120629A (en) * | 2018-03-08 | 2018-06-05 | 合肥职业技术学院 | One kind can be protected from light staining jar |
| CN208621625U (en) * | 2018-08-14 | 2019-03-19 | 南京融康博生物科技有限公司 | A kind of leukemia drug susceptibility detection kit |
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