CN208607206U - Experimental provision for chemical damage detection - Google Patents

Abstract

The utility model discloses a kind of experimental provisions for chemical damage detection, including pedestal, first platen, second platen, driving mechanism and several centrifugal units, first table stand, which is set, to be fixed on the base, the centrifugal unit includes concentrator bowl, rotation axis and driving gear, several centrifugal units are in concentrically surround to be arranged on the first platen, the rotation axis is rotatably arranged on the first platen, the structure design of the utility model is reasonable, can synchronization process multiple groups contrast sample slurries, and to the ultrasonic shatter and centrifugally operated that slurries synchronize, it is easy to operate, so that multiple groups contrast sample environmentally operates same, it ensure that the accuracy for obtaining sample, the efficiency of slurries preparation is also improved simultaneously.

Description

Experimental provision for chemical damage detection

Technical field

The utility model relates to a kind of experimental provisions for chemical damage detection.

Background technique

Drug ingedient is always component part important in preclinical medicine to the toxicity research of liver organization, wherein acetyl ammonia Base phenol (APAP) is clinically most common ntipyretic analgesic medicine, since large dosage can lead to hepatotoxicity wind agitation, at present in the U.S. and The countries such as Britain, APAP become the main reason for leading to acute liver failure, and in China, paracetamol is clinically right The research of hepatic injury is gradually paid attention to, in basic medical research, need paracetamol same dosage and with difference Drug combined use fully understands the degree of impairment of liver cell, brings liver to patient to avoid the application for occurring unreasonable The side effects such as cellular damage.

The detection of currently used chemical liver generally carries out stomach-filling drug using mature male mice, takes its hepatic tissue Liver homogenate liquid is prepared into for the detection of each index to judge the degree of hepatic injury, routine operation need to liver homogenate liquid into The centrifugation of the multiple different rates of row, obtains supernatant and precipitating is used for the detection of sample, due to convention control's experiment reagent list Solely operation, ultrasonication time, centrifugation time, centrifugation rate and environmental factor can not be completely the same, to the standard of sample results True property brings certain error.

Utility model content

The purpose of the utility model is to overcome the above deficiencies in the existing technologies, and provide a kind of structure design conjunction Reason, the experimental provision for chemical damage detection.

The technical scheme in the invention for solving the above technical problem is: a kind of reality for chemical damage detection Experiment device, including pedestal, the first platen, the second platen, driving mechanism and several centrifugal units, first table stand are set It fixes on the base, the centrifugal unit includes that concentrator bowl, rotation axis and driving gear, several centrifugal units are in It concentrically surrounds and is arranged on the first platen, the rotation axis is rotatably arranged on the first platen, one end of the rotation axis It is connected and fixed with the bottom of concentrator bowl, the axis hole of the driving gear is arranged the other end for being fixed on rotation axis, the drive Motivation structure includes driving motor, drive shaft and fluted disc, and the driving motor is fixed on the base, the axis hole of the fluted disc It is arranged and fixes on the driving shaft, one end of the drive shaft is fixed by the transmission shaft of shaft coupling and driving motor, described Fluted disc successively engaged with the driving gear of several centrifugal units, be provided with opening at the top of the concentrator bowl, described Two platens are arranged on the first platen by support frame, and the support frame includes support column, adjustable column and standing screw, institute The support column stated is fixed on the first platen, and described adjustable column one end is slidably arranged in the cavity of support column, the tune The other end of segmented column is fixed with the second platen, and the screw rod is threaded through in the sliding slot of adjustable column, the cavity of the support column Bottom be provided with the screw hole with several cooperations, the lower end surface of second platen is provided with to be cooperated for being open with concentrator bowl Sealing cover, the ultrasonication bar for being equipped with the shatter instrument of ultrasound is provided through towards sealing cover by the second platen upper end Mounting groove so that ultrasonication bar is protruded into the inner cavity of concentrator bowl by mounting groove, the sealing cover passes through first bearing and rotates The lower end surface of second platen is set.

It is further: to be provided with spring between the cavity bottom surface of the adjustable column lower end surface and support column.

Further: the sealing cover is arranged with the sealing ring with the opening cooperation of concentrator bowl.

Further: the other end of the drive shaft is connect by second bearing with the lower rotation of the first platen.

Further: the concentrator bowl is constituted using transparent material.

The utility model compared with prior art, has the following advantages that and effect: it is reasonable in design, can synchronization process it is more The slurries of group contrast sample, and to the ultrasonic shatter and centrifugally operated that slurries synchronize, it is easy to operate, so that multiple groups compare Sample is operated on same environment (centrifugation rate, time, ambient temperature and humidity etc.), ensure that the accuracy for obtaining sample, The efficiency of slurries preparation is also improved simultaneously.

Detailed description of the invention

Fig. 1 is structural schematic diagram of the utility model embodiment for the experimental provision of chemical damage detection.

Fig. 2 is the attachment structure schematic diagram of the utility model embodiment driving mechanism and centrifugal unit.

Specific embodiment

The utility model is described in further detail with reference to the accompanying drawing and by embodiment, and following embodiment is pair The explanation of the utility model and the utility model is not limited to following embodiment.

Referring to Fig. 1-Fig. 2, a kind of experimental provision for chemical damage detection of the present embodiment, including pedestal 1, first Platen 2, the second platen 3, driving mechanism 4 and several centrifugal units 5, the first platen 2 erection are fixed on pedestal 1, The centrifugal unit 5 includes concentrator bowl 51, rotation axis 52 and driving gear 53, and several centrifugal units 5 are in concentric Around being arranged on the first platen 2, the rotation axis 52 is rotatably arranged on the first platen 2, and the one of the rotation axis 52 It holds and is connected and fixed with the bottom of concentrator bowl 51, the axis hole of the driving gear 53 is arranged the other end for being fixed on rotation axis 52, The driving mechanism 4 includes driving motor 41, drive shaft 42 and fluted disc 43, and the driving motor 41 is fixed on pedestal 1 On, the axis hole of the fluted disc 43 is arranged and is fixed in drive shaft 42, one end of the drive shaft 42 by shaft coupling with The transmission shaft of driving motor 41 is fixed, and the fluted disc 43 is successively engaged with the driving gear 53 of several centrifugal units 5, described The top of concentrator bowl 51 is provided with opening, and second platen 3 is arranged on the first platen 2 by support frame, the branch Support includes support column 61, adjustable column 62 and standing screw 63, and the support column 61 is fixed on the first platen 2, described 62 one end of adjustable column be slidably arranged in the cavity 65 of support column 61, the other end of the adjustable column 62 and the second platen 3 Fixed, the screw rod is threaded through in the sliding slot of adjustable column 62, if the bottom of the cavity 65 of the support column 61 be provided with The screw hole 64 of dry cooperation, the lower end surface of second platen 3 are provided with for the sealing cover 31 with the opening cooperation of concentrator bowl 51, The peace of the ultrasonication bar 7 for being equipped with the shatter instrument of ultrasound is provided through towards sealing cover 31 by 3 upper end of the second platen Tankage 32, so that ultrasonication bar 7 is protruded into the inner cavity of concentrator bowl 51 by mounting groove 32, the sealing cover 31 passes through first bearing 33 are rotatably arranged on the lower end surface of the second platen 3, set between 62 lower end surface of adjustable column and 65 bottom surface of cavity of support column 61 It is equipped with spring 66, the sealing cover 31 is arranged with the sealing ring 34 with the opening cooperation of concentrator bowl 51, the drive shaft 42 The other end connect with the lower rotation of the first platen 2 by second bearing 44, the use transparent material texture of concentrator bowl 51 At the mode of operation of the present embodiment are as follows: the liver of the mouse of the multiple groups contrast sample of acquisition is prepared into liver homogenate liquid, is successively set It sets in corresponding concentrator bowl 51, is equipped in screw hole 64 by screw rod at this time, so that sealing cover 31 and concentrator bowl 51 Opening cooperation sealing, driving motor 41 rotate, and each concentrator bowl 51 is driven to rotate, and rate 8000 to 10000r/min is centrifuged 15- 20min takes supernatant that the present apparatus is being used to be centrifuged 20min with 4000r/min, taken its supernatant, will obtained after centrifugation after the completion Precipitating the microsomes that low-temperature homogenate medium is prepared into suspension and 7 pairs of the ultrasonication bar precipitatings by the shatter instrument of ultrasound are added It is crushed, the slurries of acquisition can be used for measuring CYP450 (predominantly cyp2e1), thus according to as a result, assessing drug to liver Operative condition, the structure design of the utility model is reasonable, can synchronization process multiple groups contrast sample slurries, and slurries are carried out same The ultrasonic shatter and centrifugally operated of step, it is easy to operate, so that multiple groups contrast sample is in same environment (centrifugation rate, time, ring Border temperature and humidity etc.) on operated, ensure that obtain sample accuracy, while also improve slurries preparation efficiency.

Above content is only illustrate to examples for clearly illustrating the present invention described in this specification.The utility model Person of ordinary skill in the field can make various modifications or additions to the described embodiments or using class As mode substitute, content without departing from the utility model specification or surmount model defined in the claims It encloses, all should belong to the protection range of the utility model.

Claims (5)

1. a kind of experimental provision for chemical damage detection, it is characterised in that: including pedestal, the first platen, second Plate, driving mechanism and several centrifugal units, first table stand, which is set, to be fixed on the base, and the centrifugal unit includes Concentrator bowl, rotation axis and driving gear, several centrifugal units are in concentrically surround to be arranged on the first platen, described Rotation axis is rotatably arranged on the first platen, and one end of the rotation axis and the bottom of concentrator bowl are connected and fixed, the drive The axis hole of moving gear is arranged the other end for being fixed on rotation axis, and the driving mechanism includes driving motor, drive shaft and tooth Disk, the driving motor is fixed on the base, and the axis hole of the fluted disc is arranged and fixes on the driving shaft, the driving One end of axis is fixed by the transmission shaft of shaft coupling and driving motor, the driving gears of the fluted disc and several centrifugal units according to Secondary engagement is provided with opening at the top of the concentrator bowl, and second platen is arranged on the first platen by support frame, The support frame includes support column, adjustable column and standing screw, and the support column is fixed on the first platen, described Adjustable column one end is slidably arranged in the cavity of support column, and the other end of the adjustable column is fixed with the second platen, described Screw rod is threaded through in the sliding slot of adjustable column, and the bottom of the cavity of the support column is provided with the screw hole with several cooperations, described The second platen lower end surface be provided with for concentrator bowl opening cooperation sealing cover, by the second platen upper end towards sealing cover It is provided through the mounting groove of the ultrasonication bar for being equipped with the shatter instrument of ultrasound, so that ultrasonication bar is stretched by mounting groove Enter the inner cavity of concentrator bowl, the sealing cover is rotatably arranged on the lower end surface of the second platen by first bearing.

2. the experimental provision according to claim 1 for chemical damage detection, it is characterised in that: the adjusting Spring is provided between column lower end surface and the cavity bottom surface of support column.

3. the experimental provision according to claim 1 for chemical damage detection, it is characterised in that: the sealing Lid is arranged with the sealing ring with the opening cooperation of concentrator bowl.

4. the experimental provision according to claim 1 for chemical damage detection, it is characterised in that: the driving The other end of axis is connect by second bearing with the lower rotation of the first platen.

5. the experimental provision according to claim 1 for chemical damage detection, it is characterised in that: the centrifugation Cylinder is constituted using transparent material.