A kind of micro fluid reaction device based on surface acoustic wave
Technical field
The utility model relates to microfluidic control technical field more particularly to a kind of micro fluid reactions based on surface acoustic wave
Device.
Background technique
The principle of WESTERN BLOT (WB) method carries out gel electrophoresis primarily with respect to protein, then in electrophoresis strip
The Binding experiment for carrying out antibody is taken, the combination of usual antibody needs gel film to place in a reservoir, then infuses in container
Enter antibody, the protein binding that container makes antibody and gel fullys shake.It usually requires to combine primary antibody and secondary antibody.Combining primary antibody
Afterwards, it needs to clean gel film and container, to reduce pollution, while the antibody after cleaning can give over to and test next time
It continues to use.The demand of WB chip in order to reduce the dosage of antibody, therefore based on microfluid is increasingly strong, and anti-in order to improve
The joint efficiency of body, the prior art, which is generally required, vibrated using shaking table, and when being tested, we need to design and reality
The shaking table for matching and using is tested, the larger use of shaking table volume is also inconvenient, and the utility model is mentioned in micro-fluid chip designs
Traditional shaking table is replaced using SAW device device out.
Utility model content
In view of the above deficiencies, the utility model provides a kind of micro fluid reaction device based on surface acoustic wave, instead of passing
Unite the use of shaking table, by the vibration of sound wave improve the reaction speed of antibody and antigenic solution in micro-fluid chip, from
And achievees the effect that shaking table and vibrate.
The utility model is achieved through the following technical solutions: a kind of micro fluid reaction device based on surface acoustic wave,
Including support plate, rotating shaft holder, limited block, spring fastener, micro-fluid chip, SAW device and cover board, on the support plate
Equipped with groove, SAW device is arranged in groove, and support plate is cuboid, and rotating shaft holder and limited block are arranged on support plate
Surface, spring fastener are connect with support plate, are arranged in support plate side surface, and the micro-fluid chip is mounted between support plate and cover board,
Through limited block clamping lock on support plate, the SAW device be integrated circuit board, including oscillating circuit, connector module with
And surface acoustic wave chip, the oscillating circuit are connected by connector module with surface acoustic wave chip, oscillating circuit is by three poles
Pipe Q4, triode Q5, resonator G6, LC antiresonant circuit and output port J9 are constituted.
As a kind of optimal technical scheme of the utility model, the LC antiresonant circuit includes inductance L1, capacitor C2
With capacitor C3, triode Q5 emitter ground connection, triode Q5 collector connecting triode Q4 emitter, the collector of triode Q4
Divide three tunnels, the first via is connected to the collector of triode Q4 by capacitor C3, capacitor C2, and the second tunnel is connected to the base of triode Q4
Pole, third road pass through inductance L1 connection output end by resonator G6, the collector of inductance connection to triode Q4, resonator G6
The end SW IN of mouth J9.
As a kind of optimal technical scheme of the utility model, the connector module includes resistance R7, resistance R8 and connects
1 foot of plug-in unit chip J10, the connector chip J10 is grounded, and 2 feet are the input terminal of output end connection sound surface watch core piece, and 3
Foot is separately connected one end of resistance R7, resistance R8, another termination+3.3V power supply of resistance R8, and the resistance R7 other end connects output end
The end PA of mouth J9.
As a kind of optimal technical scheme of the utility model, 1 foot and 2 feet of the surface acoustic wave chip are signal input
End, can be connected with 2 feet of connector chip J9, and 3 feet ground connection, 4 feet and 5 feet are signal output end, respectively with micro-fluid chip
Signal input part be connected.
As a kind of optimal technical scheme of the utility model, the surface acoustic wave chip is AF389A14DC.
As a kind of optimal technical scheme of the utility model, the plug connector chip J10 is XH2.54-3P-W.
Compared with prior art, the utility model has the following beneficial effects: using sound during microfluidic systems antibody response
Surface wave vibration replaces traditional shaking table to vibrate, on the one hand, and the movement that can accelerate fluid, which achievees the effect that increase, to be chemically reacted,
On the other hand, SAW device is located at below micro-fluid chip, can effectively help to clean remaining primary antibody, secondary antibody, assists
The cleaning of gel film and container, apparatus structure is simple, convenience and high-efficiency, wherein SAW device is integrated circuit board, utilizes crystalline substance
High frequency electrical signal generator circuit, inductance L1 and capacitor C2 are formed using crystal resonator, C3 forms the shunt-resonant circuit LC, high
Frequency electric signal becomes surface acoustic wave signal by SAW device, propagates along the surface of solids, input and output impedance error is small,
Transmission loss is small, electromagnetism interference performance good (EMI), reliability.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model;
Fig. 2 is the circuit diagram of the utility model.
Reference signs list: 1, support plate;2, rotating shaft holder;3, micro-fluid chip;4, SAW device;5, cover board;
6, limited block;7, spring fastener.
Specific embodiment
With reference to the accompanying drawings and detailed description, the present invention is furture elucidated, it should be understood that following specific embodiments are only
For illustrating the present invention rather than limiting the scope of the invention.It should be noted that word " preceding " used in the following description,
" rear ", "left", "right", "up" and "down" refer to that the direction in attached drawing, word "inner" and "outside" refer respectively to direction or remote
Direction from geometric center of specific component.
Embodiment: as shown in Figure 1, a kind of micro fluid reaction device based on surface acoustic wave, including support plate 1, swivel bearing
Seat 2, limited block 6, spring fastener 7, micro-fluid chip 3, SAW device 4 and cover board 5, support plate 1 are equipped with groove, sound surface
Wave device 4 is arranged in groove, and support plate 1 is cuboid, and rotating shaft holder 2 and limited block 6 are arranged in 1 upper surface of support plate, spring
Clip 7 is connect with support plate 1, is arranged in 1 side surface of support plate, micro-fluid chip 3 is mounted between support plate 1 and cover board 5, passes through limit
SAW device 4 is arranged the bottom of lower micro-fluid chip 3, increases microfluid by surface acoustic wave by 6 clamping lock of block on support plate
System antibody response efficiency, while the cleaning of gel film and container can be assisted, device is simple, easy to use efficient.
In the utility model, as described in Figure 2, SAW device is integrated circuit board, including oscillating circuit, connector core
Piece J10 and surface acoustic wave chip J11, oscillating circuit are connected by connector chip J10 with surface acoustic wave chip J11,
In, surface acoustic wave chip J11 be AF389A14DC, plug connector chip J10 be XH2.54-3P-W, oscillating circuit by triode Q4,
Triode Q5, resonator G6, LC antiresonant circuit and output port J9 are constituted, wherein and resonator G6 is crystal resonator,
LC antiresonant circuit includes inductance L1, capacitor C2 and capacitor C3, triode Q5 emitter ground connection, the connection of triode Q5 collector
Triode Q4 emitter, three tunnels of collector point of triode Q4, the first via are connected to triode Q4's by capacitor C3, capacitor C2
Collector, the second tunnel are connected to the base stage of triode Q4, and third road passes through resonator G6, the current collection of inductance connection to triode Q4
Pole, the 3 feet ground connection of output port J9, the collector of 2 foot connecting triode Q4,1 foot is vibrating circuit output end.
High frequency electrical signal generator circuit is formed using crystal resonator, wherein triode Q4 plays high-frequency resonant amplification and makees
With triode Q5 plays the role of current source, and inductance L1 and capacitor C2, C3 form the shunt-resonant circuit LC, and high frequency electrical signal passes through
SAW device becomes surface acoustic wave signal, propagates along the surface of solids, input and output impedance error is small, transmission loss is small,
Electromagnetism interference performance good (EMI), high reliablity.
Finally, it should be noted that being not limited to this reality the foregoing is merely the preferred embodiment of the utility model
With novel, although the utility model is described in detail with reference to the foregoing embodiments, come for those skilled in the art
Say, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic into
Row equivalent replacement.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on, should all
It is included within the scope of protection of this utility model.