CN208454986U - Cell culturing bracket and its device - Google Patents
Cell culturing bracket and its device Download PDFInfo
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- CN208454986U CN208454986U CN201820082466.4U CN201820082466U CN208454986U CN 208454986 U CN208454986 U CN 208454986U CN 201820082466 U CN201820082466 U CN 201820082466U CN 208454986 U CN208454986 U CN 208454986U
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Abstract
The utility model provides a kind of cell culturing bracket and its device, is related to technical field of cell culture, to solve the technical issues of existing cell culturing bracket is unfavorable for its pick-and-place.It is related to a kind of cell culturing bracket, comprising: the multi-layer fiber layer being fixedly connected sequentially from the bottom to top together, every layer of fibrous layer include the fiber filament of multiple parallel settings, and the fiber filament of adjacent two layers fibrous layer is staggered；The cell culturing bracket is equipped with the pick-and-place structure picked and placed convenient for it.It is related to a kind of cell culture apparatus, comprising: above-mentioned cell culturing bracket.The cell culturing bracket and its device are convenient for the pick-and-place of bracket, and are conducive to cell homoepitaxial.
Description
Technical field
The utility model relates to technical field of cell culture, more particularly, to a kind of cell culturing bracket and its device.
Background technique
Organizational project is the bioengineering operated in cell and tissue, is directed generally to the formation and again of tissue and organ
Raw, it is the revolution to tissue in surgery, organ defect and dysfunction traditional therapy and mode, at present
Organizational project has obtained swift and violent development, has obtained important breakthrough in many aspects.Cell culture invitro technology is organizational project
The vital technology in field.
Two-dimentional cell culture is a kind of conventional method of Cell culture invitro, is widely applied, is easy to operate low with expense.But
Be, two-dimensional surface culture can not analogue body inner cell grow microenvironment, be unfavorable for the friendship of iuntercellular and cell and extracellular matrix
Interaction, influence cell Proliferation, Differentiation and functional expression, and two-dimentional culture environment makes cell culture area also by one
Fixed limitation.Therefore, the inexorable trend that good dimensional culture environment is Cell culture invitro research is provided for cell.
The external structure of different engineered tissues, engineering organ requires three dimensional biological supporting structure different.It closes
The characteristics such as suitable bracket aperture, pass, designed porosity and good connectivity, specific surface area, permeability are three-dimensional cell trainings
Support the prerequisite condition of bracket.Much studies have shown that the dimensional culture bracket with suitable aperture and pass structure not only has
Microenvironment is grown conducive to cell similar with in-vivo tissue is formed, so that iuntercellular is formed suitable spatial distribution and is connected with cell,
And it is conducive to inducing cell directed differentiation and maintains cell phenotype and function.In general, the size in bracket aperture should cultivate at place
2.5 times or more of cell.For example, the bracket that aperture is 40-100 μm can promote non-mature bone and its cells adhesion and migration, maintain
Cell surface feature；Mature bone and its cells need 100-350 μm of bracket aperture；Fibroblast suitable growth is in aperture 15-
150 μm of bracket etc..In conclusion the building of ideal Three-dimensional cell culture bracket, be engineered 3D cell culture at
The precondition of function.
Currently, the technology of preparing towards Three-dimensional cell culture bracket has: freeze-drying, method of electrostatic spinning, pore creating material method
Deng, but these Three-dimensional cell culture bracket preparation methods remain problem, such as freeze-drying and method of electrostatic spinning, deposit
The inhomogenous problem rambunctious in bracket aperture is being prepared, there are pore creating material residuals for pore creating material method.3 D-printing is based on rapid shaping
Layered manufacturing principle, material/cell can be assembled according to Design Orientation to be formed customize string diameter, aperture, pass three-dimensional knot
Structure provides new technology to manufacture heterogeneous, labyrinth medical instrument and histoorgan.
However, in the prior art, Three-dimensional cell culture bracket is simple fiber filament serrated vertical design, it is unfavorable for
Cell is evenly affixed on fibrous layer, and cell is easy to slide from the vertical hole of bracket to frame bottom, while excessively matching
The bracket of culture apparatus is unfavorable for the pick-and-place of bracket when liquid or analysis detection are changed in culture.
Utility model content
The first of the utility model is designed to provide a kind of cell culturing bracket, to solve existing cell culturing bracket not
The problem of conducive to its pick-and-place.
Cell culturing bracket provided by the utility model, comprising: the multilayer being fixedly connected sequentially from the bottom to top together is fine
Layer is tieed up, the parallel setting of fibrous layer described in multilayer, every layer of fibrous layer includes the fiber filament of multiple parallel settings, and adjacent two
The fiber filament of the layer fibrous layer is staggered.
The cell culturing bracket is equipped with the pick-and-place structure picked and placed convenient for it.
Further, fibrous layer described in adjacent two layers or multilayer constitutes a fiber element, in multiple fiber elements
In, described in the fiber filament in one layer of fibrous layer and other one or more at least one described fiber element
The fiber filament in fiber element is parallel and non-coplanar setting.
Further, the filametntary stagger angle in adjacent two layers fibrous layer is 180 ° of 0 °≤θ <.
Further, the cross sectional shape in the hole that multiple fiber filaments of at least two layers fibrous layer are staggered to form is more
Side shape or circle.
Further, in fibrous layer described in same layer, the spacing between the two neighboring fiber filament is aperture.
In the same fiber element, the aperture in the formed hole of the fiber filament in fibrous layer described in same layer
It is equal.
In all fiber elements, the aperture in the formed hole of the fiber filament in the fiber element is homogeneous
Deng, either, the aperture in the formed hole of the fiber filament at least one described fiber element with other or
The aperture in the formed hole of the fiber filament in multiple fiber elements is unequal.
Further, the pick-and-place structure includes opening and/or through-hole.
The opening is multiple, and is arranged on the outer peripheral surface of the cell culturing bracket, the opening side of each opening
To being arranged outwardly.
The centre of the cell culturing bracket is arranged in the through-hole.
Further, the outer surface layer of the cell culturing bracket passes through hydrophilic treated.
Further, the shape of the cell culturing bracket is multiedge cylinder or cylindrical body.
Further, the material of the cell culturing bracket is using organic-biological high molecular polymer either lithotroph material
Material.
Cell culturing bracket provided by the utility model the utility model has the advantages that
In the cell culturing bracket, including the multi-layer fiber layer being fixedly connected sequentially from the bottom to top together, each fibre
Dimension layer includes the fiber filament of multiple parallel settings, and the fiber filament of adjacent two layers fibrous layer is staggered, which forms mutually
The three-dimensional space net structure of intertexture realizes large-scale culture of the cell in the three-dimensional space；In addition, the cell culturing bracket
Upper to be provided with the pick-and-place structure picked and placed convenient for bracket, which is conducive to cell culture, replacement culture solution or cytology inspection
The pick-and-place of bracket during survey.
In addition, the cell culturing bracket also has the characteristics that scenario-frame is simple, connection relationship is reliable and is easy production.
The second of the utility model is designed to provide a kind of cell culture apparatus, to solve existing cell culturing bracket not
The problem of conducive to its pick-and-place.
Cell culture apparatus provided by the utility model, comprising: above-mentioned cell culturing bracket.
Cell culture apparatus provided by the utility model the utility model has the advantages that
Above-mentioned cell culturing bracket is set in the apparatus, wherein the specific structure of the cell culturing bracket, connection relationship
And beneficial effect etc. is described in detail in above-mentioned text, details are not described herein.
The structure size of cell culturing bracket can be designed according to cell culture apparatus (or cell culture vessel).Thin
In born of the same parents' incubation, which it is uniform to further realize cell while simplifying operation, improving space utilization rate
It is distributed in dimensional culture rack space, makes full use of Three-dimensional cell culture space, more really simulate human body three-dimensional environment, it is special
Different structure design is conducive to cell culture, change culture solution or cytology detection process in bracket pick-and-place, operation error is caused
Cell contamination risk minimize, while three-dimensional being trained according to what different cell types and its suitable growth Environment Design customized
Bracket is supported, the proliferation apoptosis differentiation and the expression of various albumen of cell are more advantageous to.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the design concept figure for the cell culturing bracket that the embodiments of the present invention provide；
Fig. 2 is the structural schematic diagram for any two fibre layers of cell culturing bracket that the embodiments of the present invention provide,
Wherein, the pick-and-place structure setting for scheming a~d is opening or through-hole；
Fig. 3 is the structural schematic diagram for any two fibre layers of cell culturing bracket that the embodiments of the present invention provide,
Wherein, the opening number for scheming a~c is respectively two, four, it is multiple；
Fig. 4 is the structural schematic diagram for the cell culturing bracket that the embodiments of the present invention provide；
Fig. 5 is the three dimensional structure diagram for the cell culturing bracket that the embodiments of the present invention provide, wherein figure a~d
Adjacent layer fiber filament spacing graded approach be respectively incremental structure from top to bottom, reduction structure, be first incremented by again reduction structure,
First successively decrease and is incremented by structure again；
Fig. 6 is the structural schematic diagram for the cell culturing bracket that the embodiments of the present invention provide, wherein figure a~b difference
For main view, the perspective view of the histiocytic culture bracket of mature bone, figure c~d is respectively fibroblastic culture bracket
Main view, perspective view.
Icon: 100- fibrous layer；200- opening；300- through-hole；110- fiber filament.
Specific embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
Field those of ordinary skill all other embodiment obtained without making creative work belongs to practical
Novel protected range.
It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular
Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
For ease of description the utility model and simplify description, rather than the mechanism of indication or suggestion meaning or element must have it is specific
Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the
One ", " second " is used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
Embodiment one
As shown in Figures 1 to 4, the present embodiment one provides a kind of cell culturing bracket, comprising: successively fixes from the bottom to top
The multi-layer fiber layer 100 to link together, the parallel setting of multi-layer fiber layer 100, each fibrous layer 100 include multiple parallel
The fiber filament 110 of the fiber filament 110 of setting, adjacent two layers fibrous layer 100 is staggered；The cell culturing bracket, which is equipped with, to be convenient for
Its pick-and-place structure picked and placed.
In the cell culturing bracket, including the multi-layer fiber layer 100 being fixedly connected sequentially from the bottom to top together, each
Fibrous layer 100 includes the fiber filament 110 of multiple parallel settings, and the fiber filament 110 of adjacent two layers fibrous layer 100 is staggered,
The bracket forms the three-dimensional space net structure being interweaved, and realizes large-scale culture of the cell in the three-dimensional space；In addition,
The pick-and-place structure picked and placed convenient for bracket is provided on the cell culturing bracket, which is conducive to cell culture, replacement training
The pick-and-place of bracket in nutrient solution or cytology detection process.
In addition, the cell culturing bracket also has the characteristics that scenario-frame is simple, connection relationship is reliable and is easy production.
It should be noted that may be replaced by from top to bottom " from the bottom to top " herein；The cell culturing bracket can be with
It is printed by 3D printing equipment, when printing the bracket using 3D printing equipment, can successively be printed from the bottom to top
The bracket out.
Above-mentioned pick-and-place structure can have a variety of structures form, and now introduction is three kinds following, but is not limited to three kinds of structures.
The first: is as shown in Fig. 2, picking and placing structure includes multiple openings on the outer peripheral surface for be arranged in the cell culturing bracket
200, the opening direction of each opening 200 is arranged outwardly, and is connected to outside.
Please continue to refer to Fig. 2, the number of the opening 200 of Fig. 2 a and Fig. 2 c is two；As shown in figure 3, Fig. 3 a~3c's opens
Mouthfuls 200 number is respectively two, four, it is multiple.
Second: as shown in Fig. 2 b and Fig. 2 d, picking and placing structure includes that the centre of the cell culturing bracket is arranged in
Through-hole 300；Since the bracket includes multi-layer fiber layer 100, the central part of every layer multi-layer fibrous layer 100 is equipped with through-hole 300, this
The through-hole 300 at place is round tube hole, thus the through-hole 300 can be regarded as multiple concentric circles and successively fold from the bottom to top or from top to bottom
Add, in addition, the through-hole 300 is not limited to round tube hole, it can also be square hole or polygonal hole etc..
The third: picking and placing structure includes above-mentioned opening 200 and above-mentioned through-hole 300, it may be assumed that in same cell culturing bracket
On be not only arranged it is multiple opening 200, but also setting through-holes 300, further facilitate the pick-and-place to cell culturing bracket.
On the basis of the above embodiments, adjacent two layers or multi-layer fiber layer 100 constitute a fiber element, in multiple fibres
It ties up in unit, the fiber filament 110 and other one or more fibers in one layer of fibrous layer 100 at least one fiber element
Fiber filament 110 in unit is parallel and non-coplanar setting.The benefit of the structure setting is to be conducive to cell to be evenly affixed to every layer of fibre
It ties up on layer 100, and since the fiber filament 110 in multiple fiber elements parallel and non-coplanar (or not face) is arranged, thus, this reality
Multiple holes and not coaxial setting formed in the cell culturing bracket in example are applied, thus cell is not easy to slide from the hole of bracket
To frame bottom, conducive to the homoepitaxial of cell, solving cell culturing bracket in the prior art is simple fiber filament
The problem of 110 serrated verticals design, cell is easy landing to the frame bottom from the vertical hole on bracket.
Wherein, Fig. 4 is that adjacent two layers fibrous layer 100 constitutes a fiber element, two neighboring in four fiber elements
The fiber filament 110 in upper one layer of fibrous layer 100 in fiber element and upper one layer of fiber filament 110 in another fiber element are flat
Capable and non-coplanar setting；The fiber filament 110 and another fiber list in next layer of fibrous layer 100 in two neighboring fiber element
Next layer of fiber filament 110 in member be parallel and non-coplanar setting.
On the basis of the above embodiments, the stagger angle of the fiber filament 110 of adjacent two layers fibrous layer 100 is 0 °≤θ <
180°。
If the stagger angle of the fiber filament 110 of adjacent two layers fibrous layer 100 in Fig. 2 a and Fig. 2 b is 90 °；It is adjacent in Fig. 2 c
The stagger angle of the fiber filament 110 of two fibre layers 100 is 60 °；The fiber filament 110 of adjacent two layers fibrous layer 100 in Fig. 2 d
Stagger angle is 120 °.
On the basis of the above embodiments, the hole that multiple fiber filaments 110 of at least two fibre layers 100 are staggered to form is cut
Face shape is polygon or round." polygon " herein can be triangle, quadrangle (including square, rectangle, water chestnut
Shape, parallelogram), hexagon etc..
Such as: as shown in figures 2 a and 2b, the stagger angle of the fiber filament 110 of adjacent two layers fibrous layer 100 is 90 °；At least
The cross sectional shape in the hole that multiple fiber filaments 110 of two fibre layers 100 are staggered to form is quadrangle.
Wherein, the stagger angle of the fiber filament 110 of adjacent three layers of fibrous layer 100 is 60 ° in Fig. 2 c, at least three layers of fibrous layer
The cross sectional shape in the hole that 100 multiple fiber filaments 110 are staggered to form is triangle；The fibre of adjacent two layers fibrous layer 100 in Fig. 2 d
The stagger angle for tieing up silk 110 is 120 °, the section shape in the hole that multiple fiber filaments 110 of at least two fibre layers 100 are staggered to form
Shape is parallelogram.
On the basis of the above embodiments, in same layer fibrous layer 100, the spacing between two neighboring fiber filament 110 is
Aperture；In same fiber element, the aperture in the formed hole of fiber filament 110 in same layer fibrous layer 100 is equal；In all fibres
It ties up in unit, the aperture in the formed hole of fiber filament 110 in fiber element is equal (see Fig. 4), and either, at least one is fine
The aperture in the formed hole of fiber filament 110 in dimension unit is formed with the fiber filament 110 in other one or more fiber elements
The aperture in hole is unequal (see Fig. 5).
Specifically, when adjacent two layers or multi-layer fiber layer 100 constitute a fiber element, institute in two neighboring fiber element
The aperture for forming hole is presented from top to bottom to be incremented by, successively decreases, being first incremented by the aperture setting for successively decreasing again or first successively decreasing again incremental, thus
It while guaranteeing to be suitble to cell growth aperture, prevents cell from falling to frame bottom, promotes cell proliferation and differentiation and albumen
Expression.
A fiber element, corresponding position in adjacent fiber unit are constituted as shown in Figure 5 a for adjacent two layers fibrous layer 100
In fiber filament 110 it is parallel and non-coplanar (or not face), the aperture in hole formed in two neighboring fiber element fibre from up to
Lower present is incremented by structure；Reduction structure is from top to bottom presented in Fig. 5 b；Fig. 5 c, which is from top to bottom presented, is first incremented by reduction structure again；Fig. 5 d
It from top to bottom presents first to successively decrease and is incremented by structure again.
Wherein, the integral thickness (either, the vertical height of bracket from the bottom to top) of cell culture structure is >=1.0mm；
Three-dimensional cell culture internal stent aperture (filling spacing) is >=15 μm；The diameter of single fiber filament 110 in cell culturing bracket
For string diameter, string diameter >=10 μm (see Fig. 4).
On the basis of the above embodiments, the shape of the cell culturing bracket is multiedge cylinder or cylindrical body.Herein " more
Prism " can be triangular prism, cube, hexagonal prisms etc..The shape of the cell culturing bracket can be according to cell culture apparatus
Shape design.
Wherein it is determined that after the shape of the good cell culturing bracket, optionally there are two the outer peripheral surface designs of bracket
Or multiple openings 200；Or a through-hole 300 is designed at the center of bracket；It is either designed simultaneously in the outer peripheral surface of bracket again
Opening 200 and a through-hole 300 is designed at the center of bracket, above-mentioned design is conducive to taking for Three-dimensional cell culture bracket
It puts.
Since cell culturing bracket is disposably to print to be manufactured using 3D printing equipment.Above-mentioned cell culture branch
Frame raw material is organic-biological high molecular polymer either inorganic bio.
Wherein, organic-biological high molecular polymer includes but is not limited to are as follows: non biodegradable polymer (including polyphenyl second
One of alkene, polyethylene, polypropylene, polycarbonate etc. are several) or biodegradable quasi polymer (including it is polylactic acid, poly-
One or more of caprolactone, poly lactic-co-glycolic acid etc.).
Inorganic bio includes but is not limited to are as follows: lithotroph ceramic-like (including calcium phosphate bioceramic, biology are living
Property one or more of glass, magnesium phosphate, aluminium oxide, zirconium oxide etc.) or metal class (including titanium, tantalum, titanium alloy, cobalt-based close
One or more of gold, niti-shaped memorial alloy etc.).
On the basis of the above embodiments, the outer surface layer of the cell culturing bracket passes through hydrophilic treated.Specifically, example
Such as: using the processing of plasma hydrophily, Thermo-sensitive processing or bracket expoeridium collagen or extracellular matrix, to improve rack surface
Bioactivity, increase cell adhesion performance.
It should be noted that needing to comprehensively consider following aspect when designing the cell culturing bracket in the embodiment:
Selection, analysis and the design of cell growth conditions are carried out to the cell that needs are cultivated first, then targetedly
, customize design Three-dimensional cell culture bracket, finally by designed customizations Three-dimensional cell culture stent model pass through three
Dimension printing device is printed, and product is completed.Concrete operation step is following (referring to Fig. 1):
To meet good biocompatibility, the material of proper fit is selected；To meet iuntercellular reciprocation and nutrition
The conveying of substance designs 100% connectivity and suitable porosity；For the load for meeting cell viscosity properties and growth factor
Expression, handles the surface of cell culturing bracket；Promote cell proliferation and differentiation, migration and cell-specific proteins to meet
Expression, design suitable aperture or pass；Designed customization Three-dimensional cell culture bracket is passed through into 3D printing equipment
It is printed, completes product.
In addition, cell culturing bracket is the design of cell customization.Cell category mentioned above includes but is not limited to be into
Fibrocyte, keratinocyte, osteoblast, cartilage cell, liver cell, mescenchymal stem cell, cardiac muscle cell, cancer cell etc.
Cell.Wherein, cell customization designs, and refers to the size and its growing environment according to different cells, and targeted design customizes
Bracket shape, pore size, pass structure Three-dimensional cell culture bracket, can farthest situation in analogue body, make thin
Born of the same parents show the mode of space multistory growth, form the institutional framework of similar internal cell, and play its function.Mentioned above
Different types of cell can be designed according to the principle of Fig. 1 and be prepared.
It is introduced for customizing mature bone and its cells culture bracket.
As shown in figure 6 a and 6b, the shape of bracket is column construction, and concentric circles, mature bone group are designed in the center of column construction
Cell culturing bracket structure is knitted since the first layer of printing, the fiber filament 110 of every two fiber element corresponding position it is parallel and
Not face.Prevent cell from falling to frame bottom while guaranteeing to be suitble to cell growth aperture, promote cell proliferation and differentiation and
The expression of albumen.
For mature bone tissue cell growth environment, the preparation material selection of Three-dimensional cell culture bracket is phosphorous, calcic simultaneously
And the calcium phosphate material with osteoconductive.
For the histiocytic size of mature bone and the circular growth rhythm of cell, the fiber of adjacent two layers fibrous layer 100
The stagger angle of silk 110 is set as 90 °.
Wherein, according to practical 200~400 μm of Grafting Cancellous Bone Bolt girder pore size, between design culture scaffold fibers silk 110
Aperture is set as 300 μm, and the string diameter of fiber filament 110 is set as 300 μm.
It is to guarantee bracket while can match bone mechanical strength, is able to satisfy the connectivity of bracket 100% and suitable
Permeability, the cell culturing bracket porosity are set as 60%.
For mature bone and its cells, three-dimensional mature bone histocyte culture backing thickness is 1.5mm.
The osteoblast dimensional culture bracket, surface carry out the processing of collagen coating, living with the surface biological for improving bracket
Property, promote the surface adhesion of skeletonization osteoblast.
It is introduced for customizing fibroblast cell culturing bracket.
As described relative to figs. 6c and 6d, the shape of fibroblast cell culturing bracket is column construction, and column construction is opposite
Setting opening 200 on two lateral walls, opening 200 are two, and the aperture in the formed hole of fiber filament 110 of adjacent fiber unit is presented
First successively decrease and is incremented by structure afterwards.It prevents cell from falling to frame bottom while guaranteeing to be suitble to cell growth aperture, promotes cell
The expression of Proliferation, Differentiation and albumen.
For fibroblastic growth environment, the preparation material selection of Three-dimensional cell culture bracket has bioactivity and can
Biodegradable polycaprolactone material.
For fibroblastic size and the circular growth pattern of cell, the fiber filament 110 of adjacent two layers fibrous layer 100
Stagger angle be set as 90 °, i.e., the pass structure of bracket is regular quadrangle.
For fibroblastic size, the aperture cultivated between scaffold fibers silk 110 is set as 80-150 μm；Fiber filament
110 string diameter is set as 150 μm.
For the connectivity and suitable permeability for meeting bracket 100%, which is set as 65%.
The fibroblast dimensional culture bracket, surface carry out the processing of skin tissue cell epimatrix coating, to improve
The surface bioactive of bracket promotes the surface adhesion of liver cell.
Wherein, three-dimensional Fibroblast cell-culture bracket with a thickness of 1.5mm.
Embodiment two
The present embodiment two provides a kind of cell culture apparatus, comprising: above-mentioned cell culturing bracket.
Above-mentioned cell culturing bracket is set in the apparatus, wherein the specific structure of the cell culturing bracket, connection relationship
And beneficial effect etc. is described in detail in above-mentioned text, details are not described herein.
The structure size of cell culturing bracket can be designed according to cell culture apparatus (or cell culture vessel).Thin
In born of the same parents' incubation, the cell culturing bracket which provides is while simplifying operation, improving space utilization rate, further
It realizes that cell is uniformly distributed in dimensional culture rack space, makes full use of Three-dimensional cell culture space, more really simulate people
Body three-dimensional environment, special construction design be conducive to cell culture, change culture solution or cytology detection process in bracket pick-and-place, will
Cell contamination risk caused by operation error minimizes, while fixed according to different cell types and its suitable growth Environment Design
The dimensional culture bracket of inhibition and generation is more advantageous to the proliferation apoptosis differentiation and the expression of various albumen of cell.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should
Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of
Technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new
The range of each embodiment technical solution of type.
Claims (9)
1. a kind of cell culturing bracket characterized by comprising the multi-layer fiber layer being fixedly connected sequentially from the bottom to top together
(100), the parallel setting of fibrous layer described in multilayer (100), every layer of fibrous layer (100) include the fibre of multiple parallel settings
It ties up silk (110), the fiber filament (110) of fibrous layer (100) described in adjacent two layers is staggered；
The cell culturing bracket is equipped with fibrous layer (100) described in pick-and-place structure adjacent two layers or multilayer convenient for its pick-and-place and constitutes
One fiber element, one layer of fibrous layer in multiple fiber elements, at least one described fiber element
(100) fiber filament (110) in it is parallel with the fiber filament (110) in other one or more described fiber elements and
Non-coplanar setting.
2. cell culturing bracket according to claim 1, which is characterized in that described in adjacent two layers fibrous layer (100)
The stagger angle of fiber filament (110) is 180 ° of 0 °≤θ <.
3. cell culturing bracket according to claim 2, which is characterized in that at least two layers fibrous layer (100) it is more
The cross sectional shape in the hole that a fiber filament (110) is staggered to form is polygon or round.
4. cell culturing bracket according to claim 3, which is characterized in that adjacent in fibrous layer described in same layer (100)
Spacing between two fiber filaments (110) is aperture；
In the same fiber element, the institute in the fiber filament (110) formed hole in fibrous layer described in same layer (100)
It is equal to state aperture；
In all fiber elements, the aperture in the fiber filament (110) formed hole in the fiber element is equal
It is equal, either, the aperture in the fiber filament (110) formed hole at least one described fiber element and other
The aperture in the fiber filament (110) formed hole in one or more fiber elements is unequal.
5. cell culturing bracket according to claim 1-4, which is characterized in that the pick-and-place structure includes opening
(200) and/or through-hole (300)；
The opening (200) is multiple, and is arranged on the outer peripheral surface of the cell culturing bracket, each opening (200)
Opening direction is arranged outwardly；
The centre of the cell culturing bracket is arranged in the through-hole (300).
6. cell culturing bracket according to claim 1, which is characterized in that the outer surface layer of the cell culturing bracket passes through
Hydrophilic treated.
7. cell culturing bracket according to claim 1, which is characterized in that the shape of the cell culturing bracket is polygon prism
Body or cylindrical body.
8. cell culturing bracket according to claim 1, which is characterized in that the material of the cell culturing bracket is using organic
Biopolymer either inorganic bio.
9. a kind of cell culture apparatus characterized by comprising the described in any item cell culture branch of the claims 1-8
Frame.
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| CN109694825A (en) * | 2019-02-20 | 2019-04-30 | 湖南省肿瘤医院 | A kind of mold for cultivating 3D cell ring |
| CN110279498A (en) * | 2019-07-23 | 2019-09-27 | 同光(昆山)生物科技有限公司 | A kind of staggered floor overlapped 3D printing orthopaedics implant and preparation method |
| CN113944003A (en) * | 2020-10-28 | 2022-01-18 | 清华大学 | Multi-scale tissue engineering composite scaffold and preparation device and preparation method thereof |
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| CN109694825A (en) * | 2019-02-20 | 2019-04-30 | 湖南省肿瘤医院 | A kind of mold for cultivating 3D cell ring |
| CN109609380A (en) * | 2019-02-21 | 2019-04-12 | 江阴新雨生物科技有限公司 | A kind of stereoscopic cell culturing bracket |
| CN109609380B (en) * | 2019-02-21 | 2024-06-04 | 秦皇岛润泽医院有限公司 | Three-dimensional cell culture support |
| CN110279498A (en) * | 2019-07-23 | 2019-09-27 | 同光(昆山)生物科技有限公司 | A kind of staggered floor overlapped 3D printing orthopaedics implant and preparation method |
| CN113944003A (en) * | 2020-10-28 | 2022-01-18 | 清华大学 | Multi-scale tissue engineering composite scaffold and preparation device and preparation method thereof |
| CN113944003B (en) * | 2020-10-28 | 2024-01-26 | 清华大学 | Multi-scale tissue engineering composite scaffold and preparation device and preparation method thereof |
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