CN208449338U - A kind of micro-fluidic chip - Google Patents
A kind of micro-fluidic chip Download PDFInfo
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- CN208449338U CN208449338U CN201820509930.3U CN201820509930U CN208449338U CN 208449338 U CN208449338 U CN 208449338U CN 201820509930 U CN201820509930 U CN 201820509930U CN 208449338 U CN208449338 U CN 208449338U
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Abstract
The utility model relates to a kind of micro-fluidic chips, including substrate and cover plate, wherein further include with the micro-valve that can control liquid flowing, and the micro-valve jack for receiving micro-valve；The substrate is equipped with reservoir and microchannel；The reservoir and microchannel are covered and are sealed by cover plate；The microchannel is connected with micro-valve jack.The features such as micro-fluidic chip design of the utility model has structure simple, easy to assembly, reusable, and the structure can be used for the permanently effective sealing of detection reagent in testing piece.Also, the situation that the microchannel design on the flow-guiding channel and substrate of micro-valve can share to avoid flow-guiding channel, so as to avoid a possibility that mutually polluting between fluid.
Description
Technical field
The utility model belongs to medical diagnosis class article technical field, is related to a kind of micro-valve and micro- that can control liquid flowing
Fluidic chip.
Background technique
In chemistry and biological study, Most experiments and test reaction are carried out under solution state.Have one at present
Kind of research tendency is, and the detection of same sample smaller and smaller to the volume requirement of reaction liquid (sample to be tested and detection reagent)
Project is more and more or throughput requirements are higher and higher, and is able to achieve the automation and controllability of experimentation.Micro-fluidic chip
It comes into being, have the reaction liquid that can operate smaller volume, Miniaturizable, higher experiment flux with it and is able to achieve automation
The characteristics of control and used more and more.Micro-fluidic chip includes substrate and the microchannel that is arranged in substrate, and is passed through
The synergistic effect of micro-valve and power device (such as pump), sample and reaction reagent are transported in micro-fluidic chip different with being refined
Functional area desired locations in micro-fluidic chip are accurately provided, to realize the test of sample.Micro-fluidic detection skill
It is primary in a miniature portable can to integrate multiple detection projects as disease detection means by a kind of revolutionary bed for art
On the micro-fluidic chip of property.
Micro-valve is the critical elements of micro-fluidic chip, is mainly used to control the accurate flowing of sample to be tested and detection reagent.
In recent years using more extensive micro-valve by can deformation film and valve seat constitute.This kind of micro-valve includes upper layer and lower layer substrate and centre
Thin polymer film, wherein have the structure of a protrusion on the bottom substrate with fluid channel, constitute valve seat.Another upper cover
Substrate has the structure of an invagination on the corresponding position of valve seat, and providing to film can be with the cavity of deformation.This micro-valve
It is to close in the initial state, the valve seat protrusion in thin polymer film and fluid channel fits at this time, and runner is closed.
When external force is on film, separate film and valve seat, runner is opened.This external force can be pneumatic (such as U.S.
8,778,282 B2 of patent US 2013/0156658 A1 and US), heat (such as the Chinese patent CN 102006936 of deformation
2015/0028235 A1 of 2006/0243934 A1 of A, United States Patent (USP) US and US), magnetic force (such as Chinese patent CN
103244734 A) and mechanical pressure (such as 8,985,547 B2 of United States Patent (USP) US).
Since film under conventional sense and valve seat contact for a long time, film and valve seat are likely to occur adhesion, thus will appear
The phenomenon that channel can not normally open when in use.Due to the presence of valve seat outstanding and the cavity that invaginates, lead to micro-fluidic chip
Thickness it is restrained, cannot achieve micromation.Opening micro-valve needs make film by certain external force, and deformation occurs, simultaneously
Another external force is needed to become to flowing fluid in runner on fluid.The mode for controlling two kinds of power simultaneously in this way will
Lead to be difficult the flowing for accurately controlling fluid.Secondly, micro-valve is after opening, film can be contacted with fluid, be led to not extensive
Returning to customs closed state, to limit the reuse of this micro-valve structure.This micro-valve is only limited to control a kind of fluid, cannot achieve more
The function that a fluid controls simultaneously, the problem of otherwise will not can avoid pollution.Crossed in design process in structure, can by
Optimize disadvantage of the mode more than a degree of make up of the structure of film, but this also increase to a certain extent it is micro-fluidic
The processing and assembling difficulty of chip.
In the application of micro-fluidic chip as the field POCT, how another generally existing difficult point of micro-fluidic chip is
It will test reagent to be built in testing piece, to realize the micromation of entire detection device.
With the surge of the in-vitro diagnosis market demand, advantage of the microflow control technique in vitro in diagnostic application is gradually highlighted
Come, is more and more paid close attention to by industry.In micro-fluidic chip application, built in the secondary sequence flowing of multiple fluid and testing piece
The control of liquid is technological difficulties generally existing at present.
Utility model content
The utility model provides the micro-fluidic chip of a kind of micro-valve based on piston principle and micro-valve control.On the one hand
By the special designing in micro-fluidic chip channel, realize pneumatic micro-fluidic chip only with a valve can be realized built-in liquid and
Sample liquid time sequence release；On the other hand it is realized by adding different micro-structures on valve micro- on micro-fluidic chip substrate
The blocking in channel, realizes the functions such as directionality conveying at connection.
Specifically, a kind of micro-valve can control liquid flowing provided by the utility model, including valve body, on the valve body
Including at least two flow-guiding channels；It is not attached to lead between each flow-guiding channel.Flow-guiding channel on the valve body phase on valve body
It is not connected between mutually.More preferably, it is also not connected between flow-guiding channel.
In some embodiments, each flow-guiding channel runs through valve body.It is, appearance of each flow-guiding channel in valve body
Face has entrance and exit, to make to be connected outside flow-guiding channel and valve body.
In some preferred embodiments, each flow-guiding channel is located in the Different Plane of valve body.The utility model
Plane refer on valve body along horizontally or vertically or the plane that is formed of unspecified angle cutting valve body, and refer not only to horizontal plane.Often
A flow-guiding channel is located in the Different Plane of valve body and refers to that each flow-guiding channel is located in any one plane of valve body, in the plane
As soon as after being provided with a flow-guiding channel, there is no other flow-guiding channels；And between these each planes with flow-guiding channel
Without intersection at flow-guiding channel, to guarantee not to be connected between flow-guiding channel.
In some specific embodiments, one flow-guiding channel has turn-around design, the flow-guiding channel in valve body
Two open ends be located in the Different Plane of valve body.It is described that there is turn-around design to refer to the entire flow-guiding channel in valve
It is not on same plane in body, but the change in direction occurs, turning is presented in channel.In some specific embodiments,
One flow-guiding channel is the annular groove being surrounded on valve outer wall.
In some preferred embodiments, micro-valve includes valve body and the first flow-guiding channel and the second flow-guiding channel, and first leads
Circulation road and the second flow-guiding channel are not attached to lead to；First flow-guiding channel is disposed through in valve body, and both ends open is opened in
On the side wall of valve body；Second flow-guiding channel is in corner design, and the first of the second flow-guiding channel is opened on the side wall of valve body,
Second is opened in valve body bottom surface or top surface.
Preferably, the first flow-guiding channel level is disposed through in valve body.
In some preferred embodiments, the corner design is in the shape of " 7 ".
In some preferred embodiments, micro-valve includes valve body and the first flow-guiding channel and the second flow-guiding channel, and first leads
Circulation road and the second flow-guiding channel staggered floor arrange and are not attached to lead to, and the first flow-guiding channel and the second flow-guiding channel are disposed through valve
In vivo, both ends open is opened on the side wall of valve body.
Preferably, the first flow-guiding channel and the second flow-guiding channel are arranged without staggered floor across, and the first flow-guiding channel and the
The projection of two flow-guiding channels is in the position that intersects.
More preferably, the first flow-guiding channel and the second flow-guiding channel level are disposed through in valve body.
It further include having forced section in the micro-valve in some preferred embodiments.
It further include application of the micro-valve on micro-fluidic chip in the utility model.
On the other hand, the utility model also provides a kind of micro-fluidic chip, including substrate and cover plate, and, it further include tool
There are the micro-valve that can control liquid flowing, and the micro-valve jack for receiving micro-valve；The substrate is equipped with reservoir and micro- logical
Road；The reservoir and microchannel are covered and are sealed by cover plate；The microchannel is connected with micro-valve jack.
In some embodiments, cover plate includes upper cover plate and lower cover slip, and upper cover plate and lower cover slip are covered each by the upper of substrate
Surface and lower surface.
In some preferred embodiments, after the micro-valve insertion micro-valve jack, moved in micro-valve jack by micro-valve
So that microchannel is closed or is connected to, or communicates microchannel with atmosphere；By micro-valve, movement makes in reservoir in micro-valve jack
Liquid is not flowed or is flow in microchannel.
Micro-valve movement in micro-valve jack is set to make microchannel quilt specifically, passing through and pressing or rotate or press and rotate micro-valve
It closes or is connected to, or communicate microchannel with atmosphere.And by pressing or rotating or press and rotating micro-valve makes micro-valve micro-
Movement makes liquid in reservoir not flow or flow in microchannel in valve jack.
In some preferred embodiments, the micro-valve includes at least two flow-guiding channels on valve body, is each led
It is not attached to lead between circulation road.
In some preferred embodiments, the microchannel has open end in micro-valve jack；Leading in the micro-valve
Circulation road connection is located at the microchannel openings end in micro-valve jack, and flow-guiding channel in micro-valve is made to be connected with microchannel.
In some preferred embodiments, each flow-guiding channel is located in the Different Plane of valve body.
In some preferred embodiments, the surface of substrate is equipped with the first reservoir, the second reservoir and the first reservoir
First microchannel of connection, the second microchannel and third microchannel, the 4th microchannel being connect with the second reservoir；Described first
Microchannel is connected with the second microchannel, and connect simultaneously with the 4th microchannel；The 4th microchannel other end connects negative pressure
Device；Micro-valve jack is located on the first microchannel；The third microchannel is open at one end to be located in micro-valve jack.
Have in some preferred embodiments, in the micro-valve be not attached to logical the first flow-guiding channel through valve body with
Second flow-guiding channel；First flow-guiding channel is beeline channel；Second flow-guiding channel is to turn to channel.
In some preferred embodiments, the corner channel is in the shape of " 7 ".
In some preferred embodiments, the micro-valve has initial position, first position and second in micro-valve jack
Position；In initial position, the first microchannel is not attached to lead to the micro-valve by micro-valve barrier, and third microchannel is in micro-valve jack
Open end is sealed by micro-valve, and the liquid of the first reservoir and the second reservoir cannot flow；For micro-valve at first position, third is micro-
Open end of the channel in micro-valve jack connect with the second flow-guiding channel of micro-valve and is connected with atmosphere, the liquid of the second reservoir
Into the 4th microchannel, the liquid of the first reservoir cannot flow；Micro-valve is at the second position, the first microchannel and the first water conservancy diversion
The liquid of channel connection, the first reservoir enters the first microchannel and the 4th microchannel, and the liquid of the second reservoir cannot flow.
In some embodiments, position change of the micro-valve in micro-valve jack is set to realize micro-valve micro- by pressing micro-valve
By the movement of initial position to first position and the second position in valve jack.
In other embodiments, position change of the micro-valve in micro-valve jack is set to realize that micro-valve exists by rotating micro-valve
By the movement of initial position to first position and the second position in micro-valve jack.
In other embodiments, micro-valve is set to be located at initial position by pressing micro-valve, it is micro- to realize by rotation micro-valve
Valve is in micro-valve jack by the movement of initial position to first position and the second position.
It further include the 5th microchannel in some preferred embodiments, on substrate；5th microchannel position open at one end
In on micro-valve jack, another end opening is connected with atmosphere.
In some preferred embodiments, there is the first water conservancy diversion for being not attached to the logical straight line through valve body in the micro-valve
Channel and the second flow-guiding channel；First flow-guiding channel and the distribution of the second flow-guiding channel staggered floor.
Preferably, the first flow-guiding channel and the second flow-guiding channel arrange without staggered floor across and are not attached to lead to, and first leads
The projection of circulation road and the second flow-guiding channel is in the position that intersects.
In some preferred embodiments, the micro-valve has initial position, first position and second in micro-valve jack
Position；In initial position, the first microchannel is not attached to lead to the micro-valve by micro-valve barrier, third microchannel and the 5th microchannel
It is sealed in the open end of micro-valve jack by micro-valve, the liquid of the first reservoir and the second reservoir cannot be flow in microchannel；
Micro-valve is at first position, the open end of third microchannel and the 5th microchannel in micro-valve jack and the second flow-guiding channel of micro-valve
It is connected to and in communication with, third microchannel is made to be connected with the second reservoir with atmosphere, it is micro- logical that the liquid of the second reservoir enters the 4th
The liquid in road, the first reservoir cannot flow；At the second position, the first microchannel is connect micro-valve with the first flow-guiding channel, the
The liquid of one reservoir enters the first microchannel and the 4th microchannel, and the liquid of the second reservoir cannot flow.
Beneficial effect
In the utility model, what the microchannel design on the flow-guiding channel and substrate of micro-valve can share to avoid flow-guiding channel
Situation, so as to avoid a possibility that mutually polluting between fluid.Also, in the design of the structure of microchannel, the utility model
Liquid flowing is controlled using aeration status, and combines the basic structure of micro-valve, realizes the multi-functional feature of a valve.To pole
Step and external hardware device needed for the earth simplifies entire fluid control.
The micro-fluidic chip design of the utility model and micro-fluidic micro-valve have structure simple, easy to assembly, repeat
The features such as use, and the structure can be used for the permanently effective sealing of detection reagent in testing piece.Also, it is described in the utility model
It is micro-valve with being easily opened and can restore closed state, it can be with chemical reagent Long Term Contact, the functions such as easy to operate.In micro-valve
Piston on add different honeycomb ducts realize microchannel on micro-fluidic chip substrate blocking, connection, realize directionality it is defeated
The functions such as send.The features such as such micro-valve has structure simple, easy to assembly, reusable.More previous micro-valve is set
It counts, structure described in this patent can be used for the permanently effective sealing of detection reagent in chip, and micro-valve can pass through the control of mechanical force
System realizes and repeatedly opens and closes that micro-valve can have channel design to realize the stream for controlling multiple channels and multiple fluids by interior
It is dynamic.
The utility model also further utilizes the control to aeration status, realizes and only uses one in pneumatic micro-fluidic chip
Built-in liquid and sample liquid time sequence release can be realized in a valve.The micro-valve honeycomb duct, which does not intersect, does not have what microchannel shared
Situation avoids a possibility that mutually polluting between different fluid.In conjunction with the basic structure of rotary micro-valve, the more function of a valve are realized
The characteristics of energy.Step and external hardware device needed for greatly simplifiing entire fluid control.
Detailed description of the invention
Fig. 1 is a microfluidic chip structure schematic diagram of the invention.
Fig. 2 is the exploded view of Fig. 1.
Fig. 3 is the schematic diagram for the connection that micro-valve blocks microchannel.
Fig. 4 is the schematic diagram that micro-valve is microchannel connection.
Fig. 5-1 is the original state schematic diagram of a micro-valve structure of the invention.
Fig. 5-2 is the cross-sectional view in the direction Fig. 5-1A-A.
Fig. 6-1 is the liquid feeding status diagram of Fig. 5-1 micro-valve.
Fig. 6-2 is the cross-sectional view in the direction Fig. 6-1A-A.
Fig. 7-1 is the sample-adding status diagram of Fig. 5-1 micro-valve.
Fig. 7-2 is the cross-sectional view in the direction Fig. 7-1A-A.
Fig. 8-1 is the original state schematic diagram of another micro-valve structure of the invention.
Fig. 8-2 is the cross-sectional view in the direction Fig. 8-1A-A.
Fig. 9-1 is the liquid feeding status diagram of Fig. 8-1 micro-valve.
Fig. 9-2 is the cross-sectional view in the direction Fig. 9-1A-A.
Figure 10-1 is the sample-adding status diagram of Fig. 8-1 micro-valve.
Figure 10-2 is the cross-sectional view in the direction Figure 10-1A-A.
Figure 11-1 is the schematic diagram of another micro-fluidic chip of the present invention.
Figure 11-2 is the substrate upper side schematic diagram of Figure 11-1 micro-fluidic chip.
Figure 11-3 is the substrate downside schematic diagram of Figure 11-1 micro-fluidic chip.
Figure 11-4 is that Figure 11-1 micro-fluidic chip removes the schematic diagram after upper cover plate.
Figure 12 is the micro-valve structural schematic diagram in Figure 11-1 micro-fluidic chip.
Figure 13-1 is the original state schematic diagram of Figure 12 micro-valve.
Figure 13-2 is the cross-sectional view in the direction Figure 13-1A-A.
Figure 14-1 is the liquid feeding status diagram of Figure 12 micro-valve.
Figure 14-2 is the cross-sectional view in the direction Figure 14-1A-A.
Figure 15-1 is the sample-adding status diagram of Figure 12 micro-valve.
Figure 15-2 is the cross-sectional view in the direction Figure 15-1A-A.
Figure 16-1 is the substrate upper side schematic diagram with the 5th microchannel.
Figure 16-2 is the downside schematic diagram of the substrate with the 5th microchannel.
Figure 16-3 is the schematic diagram removed after upper cover plate with the 5th microchannel.
Figure 17 is another micro-valve structure.
The original state schematic diagram of Figure 18-1 Figure 17 micro-valve.
Figure 18-2 is the cross-sectional view in the direction Figure 18-1A-A.
The liquid feeding status diagram of Figure 19-1 Figure 17 micro-valve.
Figure 19-2 is the cross-sectional view in the direction Figure 19-1A-A.
The sample-adding status diagram of Figure 20-1 Figure 17 micro-valve.
Figure 20-2 is the cross-sectional view in the direction Figure 20-1A-A.
Specific embodiment
In the following detailed description, the subsidiary reference word of legend is a part here, it is to illustrate this
Utility model can the mode of actable specific concrete scheme illustrate.The utility model, which is not precluded, in we can also carry out it
Its concrete scheme and the structure for changing the utility model without prejudice to the use scope of the utility model.
Such as Fig. 1, micro-fluidic chip 100 shown in Fig. 2, Fig. 3 and 4, including substrate 2, the microchannel being arranged on substrate 4,
It is covered in the cover plate 6 of substrate, the micro-valve 8 of control 4 on-off of microchannel is equipped on the access of microchannel 4, there is installation on chip
The micro-valve jack 7 of micro-valve.The micro-valve 8 includes valve body 820 and the honeycomb duct 840 being set on piston.As shown in Figs. 3-4
Embodiment in, micro-valve be push type micro-valve, the honeycomb duct 840 is the annular groove being surrounded on valve outer wall.Such as Fig. 3 institute
Show, when the non-diversion division 822 of valve body 820 is located on the access of microchannel 4, valve body blocks and seal up microchannel 4, makes fluid
It can not circulate in microchannel 4.As shown in figure 4, when the honeycomb duct 840 of micro-valve 8 is located on the access of microchannel 4, quilt before
The microchannel 4 of the non-diversion division sealing of piston is connected to, and fluid is flowed in microchannel 4 by the honeycomb duct 840 of micro-valve.In this implementation
In example, micro-valve is moved perpendicular to base material direction under the effect of external force.
In other embodiments, the micro-fluidic chip as shown in Fig. 5-1 to 7-2.It is equipped on 2 same plane of substrate third
The microchannel 401,402,403 of distribution, the intersection three microchannels are equipped with micro-valve 8.Micro-valve 8 includes valve body, in valve body
Honeycomb duct 840 be the through-hole with 120 degree of angles.The opening 8400 of honeycomb duct 840 and the microchannel on substrate are located at one
Plane, micro-valve can rotate in valve opening under the effect of external force, realize the blocking closing on substrate between any two microchannel
Or open circulation.It is the initial position of micro-fluidic chip micro-valve as shown in Fig. 5-1 and 5-2, the honeycomb duct opening 8400 of micro-valve is not
Connect with any one microchannel 401,402,403, thus the non-water conservancy diversion position 822 of micro-valve close microchannel 401,402,
403, so that fluid can not flow between three microchannels.The state as shown in Fig. 6-1 and 6-2, micro-valve is under external force
60 degree are rotated clockwise, micro-valve honeycomb duct opening 8400 is connected to respectively at microchannel 401 with 402 at this time, and the non-water conservancy diversion of micro-valve
Position 822 closes microchannel 403, and fluid is made to realize flowing between microchannel 401 and 402.The shape as shown in Fig. 7-1 and 7-2
State, micro-valve continue to rotate clockwise 60 degree under external force, and honeycomb duct opening 8400 is respectively at microchannel 402 and 403 at this time
Connection, rather than water conservancy diversion position 822 closes microchannel 401, and fluid is made to realize flowing between microchannel 402 and 403.Above-mentioned
In embodiment, microchannel 401 can be used as titer channel, and microchannel 403 is used as sample to be tested channel.At micro-fluidic chip
In Fig. 6-1 when, titer from microchannel 401 by honeycomb duct 840 flow into microchannel 402, and by microchannel 402 enter after
Continuous detection reaction chamber.When micro-fluidic chip is in Fig. 7-1 state, titer channel close, to microchannel 403 at be added
Sample to be tested, sample to be tested flows into microchannel 402 after flowing through microchannel 403 and honeycomb duct 840, and is entered by microchannel 402
Subsequent reaction chamber.
In other embodiments, as shown in Fig. 8-1 to 10-2,2 same plane of substrate of micro-fluidic chip is equipped with four
Microchannel 401,402,403,404, the intersection four microchannels are equipped with micro-valve 8.Micro-valve 8 includes valve body, and valve is intracorporal to lead
Runner 840 is through the straight line pylone of valve body, and the opening 8400 of honeycomb duct and the microchannel on substrate are located at a plane, micro-valve
It can rotate, realize the blocking closing on substrate between two microchannels of opposite sides or beat in valve opening under the effect of external force
Open circulation.The initial position of micro-fluidic chip micro-valve as shown in Fig. 8-1 and 8-2, the honeycomb duct opening 8400 of micro-valve not with appoint
What microchannel 401,402,403,404 connects, thus the non-water conservancy diversion position 822 of micro-valve close microchannel 401,402,
403,404, so that fluid can not flow between four microchannels.State as shown in figs. 9-1 and 9-2, micro-valve is in outer masterpiece
With lower 60 degree of rotation counterclockwise, micro-valve honeycomb duct 840 is connected to respectively at microchannel 401 with 403 at this time, and the non-diversion division of micro-valve
Position closes microchannel 402 and 404, and fluid is made to realize flowing between microchannel 401 and 403.As shown in figs. 10-1 and 10-2
State, micro-valve 60 degree of rotation counterclockwise again under external force, micro-valve honeycomb duct 840 is respectively at microchannel 402 and 404 at this time
Connection, and illegal non-water conservancy diversion position closes microchannel 401 and 403, and fluid is made to realize stream between microchannel 402 and 404
It is dynamic.In the present embodiment, microchannel 402 can be used as titer channel, and microchannel 401 is used as sample to be tested channel.Work as miniflow
When be in Fig. 9-1, sample to be tested is added from microchannel 401 into micro-fluidic chip control chip, and pass through honeycomb duct 840 flow into it is micro-
Channel 403, and subsequent reaction chamber is entered by microchannel 403.When micro-fluidic chip is in Figure 10-1 state, sample adds
Sample channel is closed, to microchannel 423 at titer is added, titer flows into microchannel 404 by honeycomb duct 840, and by micro-
Channel 404 enters subsequent reaction chamber.
The utility model also provides micro-fluidic chip 100 and micro-valve as shown in Figure 11-1 to 20-2, the micro-fluidic core
Piece includes substrate 2 and is covered each by upper cover plate 62 and lower cover slip 64 in substrate above and below.The table of substrate in the present embodiment
Reservoir and microchannel are offered on face respectively, after upper cover plate and lower cover slip are bonded on substrate respectively, the reservoir and
Microchannel forms closed liquid storage cavity or microchannel.
As shown in Figure 11-1 to Figure 11-4, the upper side of substrate offers the first reservoir 11, the second reservoir 12 and
One microchannel 401, the second microchannel 402, the 4th microchannel 404, the reservoir and microchannel and not through substrate lower surface.
Micro-valve 8 has the first flow-guiding channel and the second flow-guiding channel being located on valve body.It is offered on the access of the first microchannel 401
Through the micro-valve jack 7 of substrate 2 and upper and lower cover plate 62,64, the first microchannel 401 is divided into two parts by micro-valve jack 7.The
One end of one microchannel, 401 first part is connected to the first reservoir 11, and the other end is opened on micro-valve jack 7, and when in jack 7
When micro-valve 8 is in some position (second position), another end opening of 401 first part of the first microchannel can be with the of micro-valve 8
The opening 8400 of one flow-guiding channel 841 is connected to each other and is connected to, and another opening 8400 and the of the first flow-guiding channel 841 at this time
The second part of one microchannel 401 is connected to.One end of second microchannel 402 is connected to the second reservoir 12, the first microchannel 401
The other end and 402 other end of the second microchannel are connected to the 4th microchannel 404 respectively.Third is offered in the downside of substrate 2
Microchannel 403, third microchannel and not through substrate upper surface, and also not with the first reservoir 11, microchannel 401,402,404
It is directly connected to.Third microchannel 403 is the venting channels of the second reservoir, and one end is connected to the second reservoir 12, the other end
For opening towards micro-valve jack 7, and when the micro-valve 8 in jack is in some position (first position), third microchannel 403 is another
First opening 8401 of end opening and the second flow-guiding channel of micro-valve 842 is connected to each other and is connected to, so that the second reservoir can be with
Atmosphere communicates.4041 connecting power device of bleeding point positioned at 404 end of the 4th microchannel provides negative for microchannel 401-404
The environment of pressure provides the power of directed flow as the fluid in the first reservoir and the second reservoir.To which micro-valve 8 is used for
Control the fluid circulation in the first reservoir 11 and the second reservoir, and the on-off of controllable second reservoir and atmosphere.Upper cover
Jack 7 is opened up at the micro-valve jack of piece and lower cover slip counter substrate.
As shown in Figure 12 to 15-2, micro-valve 8 includes valve body 820 and the first flow-guiding channel 841 and the second flow-guiding channel 842,
First flow-guiding channel and the second flow-guiding channel are connected to without intersection.First flow-guiding channel, 841 level is disposed through in valve body, and two
End opening 8400 is opened on the side wall of valve body.And when micro-valve is located at some position (second position), the first flow-guiding channel
841 opening 8400 is connect with the first microchannel 401, so that the first microchannel separated by micro-valve passes through the first flow-guiding channel
841 are interconnected together.Second flow-guiding channel 842 is in corner design, such as the shape of similar " 7 ", the second flow-guiding channel 842
The first opening 8401 be opened on the side wall of valve body, the second opening 8402 is opened in valve body bottom surface or top surface.When micro-valve is located at
When some position (first position), the first opening 8401 of the second flow-guiding channel 842 is connected to third microchannel 403, outer at this time
Boundary's atmosphere can enter the second liquid storage through flow-guiding channel 842 and the first opening 8401 by the second opening 8402 of the second flow-guiding channel
In slot 12.It is more specific:
When micro-valve is in the initial position as shown in Figure 13-1 and Figure 13-2, the first microchannel 401 and third are micro- logical
Road 403 by the non-diversion division 822 of valve body blocking and be in closed state, at this time in the environment of negative pressure (open four-way
Pump at road 404 or other be capable of providing the power device of negative pressure), due to the second reservoir 12 not with external world's conducting in true
Sky, the liquid in reservoir 12 are can not to flow, and the first microchannel 401 of the first reservoir is blocked by micro-valve, first
Fluid can not also be circulated to the 4th microchannel in reservoir.
When micro-valve is in the first position state as shown in Figure 14-1 and Figure 14-2, the of third microchannel 403 and micro-valve
8401 connection of the first opening of two flow-guiding channels 842, the second reservoir pass through third microchannel 403, first opening 8401, micro-valve
Second flow-guiding channel 842 and its second opening 8402 are communicated with atmosphere.At this time under the negative pressure that the 4th microchannel 404 provides, the
Built-in fluid in two reservoirs 12 enters the 4th microchannel 404.
When micro-valve is in the second position state as shown in Figure 15-1 and Figure 15-2, third microchannel 403 is not led with second
Circulation road 842 communicates to be closed the ambient air passage of the second reservoir.At this time the opening 8400 of the first flow-guiding channel 841 with
The connection of first microchannel 401,401 flow path of the first microchannel are opened, at this time under the negative pressure that the 4th microchannel 404 provides, the
Fluid in one reservoir 11 enters fourth lane 404 by the first flow-guiding channel 841 in micro-valve, and the second reservoir 12 by
In not communicating with atmosphere, fluid can not be sucked out.
The utility model also provides micro-fluidic chip and micro-valve as shown in Figure 16-1 to 20-2.Figure 16's-1 to Figure 20-2
Micro-fluidic chip and micro-fluidic chip shown in Figure 11-2 to 13 are essentially identical, the difference is that further including the 5th microchannel
405 and micro-valve 8 different flow-guiding channels settings.As shown in Figure 16-1 to 16-3, the 5th microchannel 405 is arranged in base
The downside of piece 2, the 5th microchannel 405 and not through substrate upper side, and also not with the first reservoir 11, the second reservoir
12, microchannel 401,402,403,404 is directly connected to.5th microchannel 405 it is open at one end towards micro-valve jack 7, the other end
Opening is communicated with the atmosphere set on substrate side.And when the micro-valve 8 in jack is in some position (first position), the 5th is micro- logical
The opening in road 405 is connected to each other and is connected to, the opening of third channel 403 with the second opening 8402 of the second flow-guiding channel of micro-valve 842
It is connected to each other and is connected to the first opening 8401 of the second flow-guiding channel of micro-valve 842.
Meanwhile micro-valve 8 as shown in figure 17, including valve body 820 and the first flow-guiding channel 841 and the second flow-guiding channel 842,
First flow-guiding channel and the second flow-guiding channel are arranged without staggered floor across, specifically, the first flow-guiding channel and the second flow-guiding channel
Projection in intersecting position.First flow-guiding channel, 841 level is disposed through in valve body, and both ends open 8400 is opened in
On the side wall of valve body, and when micro-valve is located at some position (second position), the opening 8400 and first of the first flow-guiding channel 841
Microchannel 401 connects, so that the first microchannel separated by micro-valve is interconnected together by the first flow-guiding channel 841.
Second flow-guiding channel 842 is also that level is disposed through in valve body, with the first flow-guiding channel not on the same horizontal plane, two
End opening 8401,8402 is opened on the side wall of valve body, and when micro-valve is located at some position (first position), but is not
When one microchannel 401 is connected, an opening in the opening 8401 of the second honeycomb duct 842 is connected to third microchannel 403,
Another opening 8402 is connected to the 5th microchannel 405 of micro-fluidic chip.Ambient atmosphere can pass through the 5th microchannel 405 at this time
The opening being connected with atmosphere enters the 5th microchannel, enters the second reservoir after the second flow-guiding channel.It is specific:
When micro-valve is in the initial position as shown in Figure 18-1, the first microchannel 401, third microchannel 403 and
Five microchannels 405 are in closed state by the blocking of the non-diversion division 822 of micro-valve, (open the in the environment of negative pressure at this time
Pump at four-way 404 or other be capable of providing the power device of negative pressure), since the second reservoir 12 is not in external world's conducting
Vacuum, the liquid in reservoir 12 are can not to flow, and the first microchannel of the first reservoir is also by piston blocks, first
Fluid can not also be circulated to fourth lane in reservoir.
When micro-valve is in the first position state as shown in Figure 19-1, the opening of Five-channel and the second flow-guiding channel 842
Connection, the second flow-guiding channel is connected in third microchannel 403 again, so that the second reservoir is communicated with atmosphere.It is micro- the 4th at this time
Under the negative pressure that channel 404 provides, the built-in fluid in the second reservoir 12 enters the 4th microchannel 404.
When micro-valve is in the second position state as shown in Figure 20-1, the 5th microchannel 405 not with the second flow-guiding channel 842
It communicates to be closed the ambient air passage of the second reservoir.The opening 8400 of first flow-guiding channel 841 and first micro- logical at this time
Road 401 connects, and 401 flow path of the first microchannel is opened, at this time under the negative pressure that the 4th microchannel 404 provides, the first reservoir
Fluid in 11 enters fourth lane 404 by the first honeycomb duct 841 in micro-valve.
In comparison, micro-valve shown in Figure 12 has better performance compared to micro-valve shown in Figure 17.By Figure 12 institute
The second channel opening for showing micro-valve can be directly communicated with atmosphere positioned at the bottom surface or top surface of valve body, this can pacify reducing
Still ensure that the second flow-guiding channel is communicated with the atmosphere always in the case where dress precision.And micro-valve shown in Figure 17, need with it is micro-
It could be communicated with atmosphere after the 5th microchannel docking on fluidic chip, therefore the installation accuracy of such micro-fluidic chip
Just must be very high, if installation error is more than certain range, it is possible to lead to opening for the second flow-guiding channel in use process
Mouth can not accurately be docked with the 5th microchannel, and also just directly resulting in atmosphere cannot be introduced into the second flow-guiding channel.
For most of in-vitro diagnosis product, before being detected to sample to be tested, with built-in calibration solution (Quality Control
Liquid or titer) to instrument carry out calibration be very important step.Special structure is had by described in the utility model at one
The micro-fluidic chip of micro-valve is in the embodiment of pattern detection, first reservoir 11 to be for storing the fluid-like that need to be detected
This, storage in advance has fluid (such as titer, detection reaction reagent etc.) in the second reservoir 12.When upper cover plate is adhered to substrate
Upper side when, the first reservoir and the second reservoir are respectively formed airtight cavity.Upper cover plate 62 is with respect to 11 position of the first reservoir
Place is equipped with well 111, and additional fluid sample can be entered in the first reservoir 11 by the well 111.Micro-valve is turned first
It moves to the state for allowing the second reservoir to communicate with atmosphere, under the negative pressure that the 4th microchannel 404 provides, in the second reservoir 12
Titer is sucked into detection zone to calibrate detector.After calibration, micro-valve is rotated to the shape being connected to microchannel 401
State, the first reservoir is connected to the 4th microchannel 404 at this time, the sample to be tested liquid in the first reservoir enters detection zone, completes
Detection.A possibility that fluid part in different reservoirs mutually pollutes is avoided using flow-guiding channel dislocation design in micro-valve.Together
When, and can be using the transformation of the on off operating mode of pre- atmosphere, to control the flowing of fluid in reservoir.
Flow-guiding channel in micro-valve can be the form for the annular groove being surrounded on piston outer wall, be also possible to through
The through-hole form of piston.Flow-guiding channel set-up mode on piston includes but is not limited to both the above mode.Micro-valve is in external force
It under effect, can move in micro-fluidic chip, be flowed to close or open by the mobile blocking for realizing microchannel of micro-valve
Logical, the move mode includes but is not limited to the upper and lower displacement of micro-valve pressing mode or the movement of rotation mode.Implement at one
It further include having forced section in micro-valve 8 in example, the receiving unit 860 to concave shown in such as, but not limited to Figure 17, such as whirler
Tool arm can be combined with the receiving unit 860, accurately drive micro-valve 8 mobile.
It is suitable that the flow-guiding channel cross sectional shape of the micro-valve can be selected from round, rectangular, track type, ellipse or diamond shape etc.
Shape.
The material of micro-valve 8 can be plastics, rubber, hydrogel or certain elastic polymer material.It may be any of the above
A kind of biggish material of another relative hardness of material package is constituted.Using elastic material, piston interference filling micro-valve can be made to insert
Simultaneously realize sealing in hole.
The substrate and cover plate can be made of the material of following any one or two kinds of mixed types, such as silicon, ceramics, glass
With plastics etc., wherein the plastics are selected from: acrylonitrile-butadiene-styrene (ABS) co-polymer (ABS), cyclic olefin polymer
(COP), polyamide (PA), polybutylene terephthalate (PBT) (PBT), polycarbonate (PC), dimethyl silicone polymer (PDMS),
Polyethylene (PE), polyethylene terephthalate (PET), polymethyl methacrylate (PMMA), gathers polyether-ether-ketone (PEEK)
Formaldehyde (POM), polypropylene (PP), polystyrene diethyl ether (PPE), polystyrene (PS), polysulfones (PSU), polytetrafluoroethylene (PTFE)
(PTFE) etc..
The substrate and cover plate can be by compressing the hydrogen bonding of formation after surface modification, chemical bonding, hot pressing, gluing
Any one mode such as mixture bonding or double faced adhesive tape bonding is combined together.
The depth of microchannel on micro-fluidic chip is 0.1-5mm, width 0.1-5.0mm.
Microchannel, reservoir on micro-fluidic chip etc. can be processed in the outer surface of substrate, then on substrate in covering
The confined space that can store fluid is formed after cover plate.Microchannel, reservoir etc. on micro-fluidic chip also can be processed in substrate
Inside forms the space that can store fluid, without being covered again with cover plate to form the confined space that can store fluid.
The micro-valve jack, microchannel and honeycomb duct etc. can be processed by any mode such as micro Process or machining.
In micro-fluidic chip the sealing, release of fluid, to seal again be movement by micro-valve in valve opening, utilization is same
The different structure portion of piston realizes.Fluid realizes circulation in microchannel under the effect of external force, gives fluid circulation
External force type can be gravity, centrifugal force, aerodynamic force, surface tension etc..
Claims (11)
1. a kind of micro-fluidic chip, including substrate and cover plate, which is characterized in that further include having to can control the micro- of liquid flowing
Valve, and the micro-valve jack for receiving micro-valve；The substrate is equipped with reservoir and microchannel；The reservoir and microchannel
It is covered and is sealed by cover plate；The microchannel is connected with micro-valve jack.
2. micro-fluidic chip according to claim 1, which is characterized in that after the micro-valve insertion micro-valve jack, by micro-
Valve movement in micro-valve jack makes microchannel be closed or be connected to, or communicates microchannel with atmosphere；It is inserted by micro-valve in micro-valve
Movement makes liquid in reservoir not flow or flow in microchannel in hole.
3. micro-fluidic chip according to claim 2, which is characterized in that the micro-valve includes at least two on valve body
A flow-guiding channel is not attached to lead between each flow-guiding channel.
4. micro-fluidic chip according to claim 3, which is characterized in that the microchannel has opening in micro-valve jack
End；Flow-guiding channel connection in the micro-valve is located at the microchannel openings end in micro-valve jack, make in micro-valve flow-guiding channel with it is micro-
Channel is connected.
5. micro-fluidic chip according to claim 3, which is characterized in that each flow-guiding channel is located at the Different Plane of valve body
On.
6. micro-fluidic chip described in one of -5 according to claim 1, which is characterized in that the surface of substrate is equipped with the first liquid storage
Slot, the second reservoir, the first microchannel being connect with the first reservoir, the second microchannel being connect with the second reservoir and third
Microchannel, the 4th microchannel；First microchannel is connected with the second microchannel, and connect simultaneously with the 4th microchannel；Institute
State the 4th microchannel other end connection negative pressure device；Micro-valve jack is located on the first microchannel；It opens third microchannel one end
Mouth is located in micro-valve jack.
7. micro-fluidic chip according to claim 6, which is characterized in that have in the micro-valve be not attached to it is logical through valve
The first flow-guiding channel and the second flow-guiding channel of body；First flow-guiding channel is beeline channel；Second flow-guiding channel is to turn to
Channel.
8. micro-fluidic chip according to claim 7, which is characterized in that the micro-valve has initial bit in micro-valve jack
It sets, first position and the second position；In initial position, the first microchannel is not attached to lead to the micro-valve by micro-valve barrier, third
Microchannel is sealed in the open end of micro-valve jack by micro-valve, and the liquid of the first reservoir and the second reservoir cannot flow；Micro-valve
At first position, open end of the third microchannel in micro-valve jack connect with the second flow-guiding channel of micro-valve and is connected with atmosphere
Logical, the liquid of the second reservoir enters the 4th microchannel, and the liquid of the first reservoir cannot flow；Micro-valve at the second position,
First microchannel is connect with the first flow-guiding channel, and the liquid of the first reservoir enters the first microchannel and the 4th microchannel, and second
The liquid of reservoir cannot flow.
9. micro-fluidic chip according to claim 6, which is characterized in that further include the 5th microchannel on substrate；Described
Five microchannels are open at one end to be located on micro-valve jack, and another end opening is connected with atmosphere.
10. micro-fluidic chip according to claim 9, which is characterized in that have in the micro-valve and be not attached to logical run through
The first flow-guiding channel and the second flow-guiding channel of the straight line of valve body；First flow-guiding channel and the second flow-guiding channel staggered floor point
Cloth.
11. micro-fluidic chip according to claim 10, which is characterized in that the micro-valve has initial in micro-valve jack
Position, first position and the second position；In initial position, the first microchannel is not attached to lead to the micro-valve by micro-valve barrier, the
Three microchannels and the 5th microchannel are sealed in the open end of micro-valve jack by micro-valve, the liquid of the first reservoir and the second reservoir
It cannot flow in microchannel；Micro-valve is at first position, the opening of third microchannel and the 5th microchannel in micro-valve jack
End is connected to and in communication with the second flow-guiding channel of micro-valve, so that third microchannel is connected with the second reservoir with atmosphere, the second liquid storage
The liquid of slot enters the 4th microchannel, and the liquid of the first reservoir cannot flow；Micro-valve at the second position, the first microchannel with
The liquid of the connection of first flow-guiding channel, the first reservoir enters the first microchannel and the 4th microchannel, the liquid of the second reservoir
It cannot flow.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108443579A (en) * | 2018-04-11 | 2018-08-24 | 利多(香港)有限公司 | A kind of micro-valve and micro-fluidic chip that can control liquid flowing |
| CN110173572A (en) * | 2019-06-17 | 2019-08-27 | 凡知医疗科技(江苏)有限公司 | A kind of microfluidic control valve mechanism |
| CN110479392A (en) * | 2019-08-23 | 2019-11-22 | 江苏集萃智能传感技术研究所有限公司 | A kind of microfluidic valve and its detection chip |
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| CN108443579A (en) * | 2018-04-11 | 2018-08-24 | 利多(香港)有限公司 | A kind of micro-valve and micro-fluidic chip that can control liquid flowing |
| WO2019196850A1 (en) * | 2018-04-11 | 2019-10-17 | 利多(香港)有限公司 | Multifunctional microvalve capable of controlling flow of fluid, microfluidic chip and method |
| CN112033953A (en) * | 2019-06-03 | 2020-12-04 | 利多(香港)有限公司 | Micro-fluidic chip and application |
| CN112033953B (en) * | 2019-06-03 | 2024-04-19 | 利多(香港)有限公司 | Microfluidic chip and application |
| CN110173572A (en) * | 2019-06-17 | 2019-08-27 | 凡知医疗科技(江苏)有限公司 | A kind of microfluidic control valve mechanism |
| CN114341538A (en) * | 2019-07-12 | 2022-04-12 | 好奇诊断有限责任公司 | Micro-fluidic chip and valve, production method and application |
| CN114341538B (en) * | 2019-07-12 | 2023-01-20 | 好奇诊断有限责任公司 | Micro-fluidic chip and valve, production method and application |
| US12017220B2 (en) | 2019-07-12 | 2024-06-25 | Curiosity Diagnostics Sp. Z O.O. | Microfluidic chip and valve, production process and uses |
| CN110479392A (en) * | 2019-08-23 | 2019-11-22 | 江苏集萃智能传感技术研究所有限公司 | A kind of microfluidic valve and its detection chip |
| CN111715149A (en) * | 2020-07-11 | 2020-09-29 | 深圳市和来生物技术有限公司 | Quantitative liquid adding device and method based on time sequence control and microfluidic chip |
| CN112147356A (en) * | 2020-07-11 | 2020-12-29 | 深圳市和来生物技术有限公司 | Quantitative liquid taking mechanism and microfluidic device |
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