CN208354445U - A kind of spiral jatharapanvartanasana runner cryoprotective agent processing micro-fluid chip - Google Patents
A kind of spiral jatharapanvartanasana runner cryoprotective agent processing micro-fluid chip Download PDFInfo
- Publication number
- CN208354445U CN208354445U CN201820913427.4U CN201820913427U CN208354445U CN 208354445 U CN208354445 U CN 208354445U CN 201820913427 U CN201820913427 U CN 201820913427U CN 208354445 U CN208354445 U CN 208354445U
- Authority
- CN
- China
- Prior art keywords
- side core
- core piece
- cell
- semi
- displacement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
A kind of spiral jatharapanvartanasana runner cryoprotective agent processing micro-fluid chip, for cryoprotective agent to be added or removed to cell suspending liquid.Chip includes cell side core piece, semi-permeable membrane and displacement side core piece, and cell side core piece and displacement side core piece are fitted on semi-permeable membrane；Cell side core piece and displacement side core on piece have fluid channel, and fluid channel includes entrance, exchange section and outlet section；Cell side core piece or the exchange section for replacing side core on piece fluid channel are the identical spiral revolving structure of shape, are arranged symmetrically in semi-permeable membrane two sides.When for cryoprotective agent processing, the reverse flow in the fluid channel of semi-permeable membrane two sides of cell suspending liquid and displacement liquid forms continuous transmembrane pressure in semi-permeable membrane two sides, and driving solution passes through semi-transparent membrane flow；Cell suspending liquid is continuously concentrated or dilutes in the chips, realizes the control to the cryoprotective agent concentration in cell suspending liquid, and can avoid loss cell caused by local transmembrane pressure is mutated.
Description
Technical field
The utility model relates to cell low temperature preservation fields, protect particularly for low temperature is added or removed to cell suspending liquid
Protect the micro-fluidic chip of agent.
Background technique
Cryo-conservation is widely used in the long-term preservation of the living materials such as rare blood type red blood cell, stem cell, immunocyte.
Other processing links (such as cooling, rewarming) although cell can be saved in long-term safety under profound hypothermia, during saving
It is likely to cause biomaterial certain damage, i.e., so-called low temperature injury.In order to avoid or reduce low temperature injury, usually
Cryoprotective agent is added in cryo-conservation process, and removes cryoprotective agent during recovery frozen cell.
The method that traditional cryoprotective agent addition or removal are mainly based upon centrifuge separation, but there are a series of for this method
Problem: on the one hand, this method is usually comparatively laborious, for example, a unit red blood cell removal cryoprotective agent operation time-consuming
Up to several hours；On the other hand, treatment process is easy to cause a large amount of loss cell, such as reports navel according to Antonenas et al.
The ratio that karyocyte loses during removing cryoprotective agent after rewarming with hemocytoblast is up to 27.2% (Bone
Marrow Transplantation,2004,34,739)。
For solve centrifugal cryogenic protective agent processing method relevant issues, Chinese patent ZL200720037535.1 and
ZL201210032654.3 successively proposes the UF membrane formula cryoprotective agent processing method with dialysis and ultrafiltration principle.But this
A little macroscopical flow devices of method application (especially commercial dialyzer, ultrafilter) are handled, to the finite volume of process object
System, it is difficult to handle small size object.Some documents are proposed using micro-fluidic formula cryoprotective agent processing method, can handle small
Volume object, but mass-transfer efficiency is poor, and processing flux is low, such as Song is using the micro-fluidic processing system with S-shaped runner, processing
Flux be only 2 mul/min (Lab on a Chip, 2009,9,1874-1881).
The combination of membrane separation process and microfluidic method is expected to better adapt to the process demand of small size object, that is, passes through miniflow
Body realizes that safe and efficient cryoprotective agent is handled in the flowing of semi-permeable membrane two sides and cross-film mass transfer.But the design of fluid channel is same
It is very crucial, because on the one hand the topological structure of runner affects overall mass-transfer efficiency, on the other hand have an effect on film two sides
Transmembrane pressure is distributed and then influences cell safety.Traditional straight channel or S-shaped runner can not really play both methods knot
The advantage of conjunction.The main problem of straight channel is, straight channel length and the film surface that can be utilized under limited overall dimension
Product is limited, causes the mass-transfer efficiency of system still insufficient.Such as Lusianti and Higgins is using micro-fluidic with straight channel
System, emulation and experiments have shown that need multistage series winding also just realize sufficient cryoprotective agent processing (Biomicrofluidics,
2014,8,054124).The main problem of S-shaped runner is, there are barometric gradients and corresponding at each revolution position for runner
Transmembrane pressure mutation, lead to that there are uncontrollable hidden danger in the position cell safety.
Summary of the invention
The purpose of the utility model is to provide a kind of spiral jatharapanvartanasana runner cryoprotective agents to handle micro-fluid chip, uses
The runner topological structure of spiral jatharapanvartanasana improves processing effect so that obtaining bigger flow channel length under limited overall dimension
Rate, and uniform barometric gradient in runner is maintained, cellular damage risk caused by avoiding transmembrane pressure from being mutated.
The spiral jatharapanvartanasana runner cryoprotective agent handles micro-fluidic chip, including cell side core piece, semi-permeable membrane and sets
Side change chip, cell side core piece and displacement side core piece are fitted on semi-permeable membrane；Cell side core piece and displacement side core on piece have
Fluid channel, and fluid channel includes entrance, exchange section and outlet section；The friendship of cell side core piece and displacement side core on piece fluid channel
Changing section is the identical spiral revolving structure of shape, is arranged symmetrically in semi-permeable membrane two sides；Semi-permeable membrane is same spiral revolving structure,
And width is greater than cell side core piece and replaces the width of flow path of side core on piece；The entrance of cell side core piece is connected to cell suspending liquid
Entrance, cell side core piece outlet section are connected to cell suspending liquid outlet, and the entrance of displacement side core piece is connected to displacement liquid entrance, displacement
The outlet of the outlet section connection displacement liquid of side core piece.When for cryoprotective agent processing, cell suspending liquid and displacement liquid are half
Reverse flow in the fluid channel of permeable membrane two sides forms continuous transmembrane pressure in semi-permeable membrane two sides, and driving solution passes through semi-transparent membrane flow；
When pressure is greater than pressure in displacement side core piece fluid channel in cell side core piece fluid channel, the extracellular solution of cell suspending liquid is to displacement
Liquid stream is dynamic, and cell is retained by semi-permeable membrane, and cell suspending liquid is concentrated；When pressure is less than displacement side core in cell side core piece fluid channel
Pressure in piece fluid channel, displacement liquid are flowed to cell suspending liquid, and cell suspending liquid is diluted；In continuous concentration or diluting effect
Under, realize the control to the cryoprotective agent concentration in cell suspending liquid.
The axis of the spiral revolving structure is made of several connected and tangent semi arches, can be divided into A group and B group；A group
All semi arches are concentric, and innermost layer, that is, first layer semicircle arc radius is R1, and radius differs 2 times of R1 between adjacent each layer；B group institute
Have that semi arch is concentric, first layer semicircle arc radius is also R1, and the center of circle of distance of center circle A group semi arch is 2 times of R1；The first of A group
The first layer semi arch of layer semi arch one end connection B group, the other end connect the second layer semi arch of B group；The second layer semicircle of A group
Arc one end connects the first layer semi arch of B group, and the other end connects the third layer semi arch of B group；Second layer semi arch one end of B group
The first layer semi arch of A group is connected, the other end connects the third layer semi arch of A group.
The cell side core piece and replace side core piece fluid channel entrance and outlet section be straight channel, axis with it is micro-
The axis of the exchange section of runner is tangent；Outlet section of the entrance of cell side core piece fluid channel close to displacement side core piece, cell side
Entrance of the outlet section of chip close to displacement side core piece.
The semi-permeable membrane is plane microfiltration membranes, and thickness is less than 200 microns, and aperture is less than 0.22 micron, gabarit full-size
Between 10~300 millimeters, material is mixed cellulose ester or polyether sulfone.
Between 0.1 millimeter to 0.5 millimeter, width is situated between the depth of the fluid channel of the cell side core piece and displacement side core on piece
In 0.5 millimeter to 10 millimeters, length is between 100 millimeters to 1000 millimeters.
The cell side core piece, displacement side core piece and semi-permeable membrane are superimposed with sandwich structure, and semi-permeable membrane is in cell side core
Between piece and displacement side core piece, whole chip is assembled to form by macromolecule glue bonding.
The advantageous effects of the utility model are:
(1) under same overall dimension, the back-shaped runner of the spiral of chip described in the utility model has more than straight channel
Big flow channel length correspondingly obtains more fully film exchange mass transfer；In combination with the biggish transparency of microfiltration membranes, so that carefully
Sufficient cryoprotective agent processing can be realized in born of the same parents' suspension under the conditions of once-through.
(2) barometric gradient in chip fluid channel described in the utility model is evenly distributed, and cell is undergone outer in the chips
Environment seepage bucklingization is gentle, cell safety issue caused by avoiding the local transmembrane pressure of conventional flow field (such as S-shaped runner) from being mutated.
(3) by using various sizes of semi-permeable membrane and runner, it can flexibly realize that several tens of microliters is outstanding to hundreds of milliliters of cells
The addition or removal cryoprotective agent processing of supernatant liquid.
Detailed description of the invention
Fig. 1 is the schematic illustration of cryoprotective agent processing chip described in the utility model
Fig. 2 is the cell side micro-channel structure schematic diagram of embodiment
Fig. 3 is the displacement side micro-channel structure schematic diagram of embodiment
Fig. 4 is the semi-transparent membrane structure diagram of embodiment
Fig. 5 is that when adding cryoprotective agent, pressure is distributed embodiment in chip runner
Fig. 6 is cross-film mobility status of the embodiment when adding cryoprotective agent
Fig. 7 is that embodiment cell when adding cryoprotective agent hangs cryoprotective agent concentration distribution in side runner
Fig. 8 is that when removing cryoprotective agent, pressure is distributed embodiment in chip runner
Fig. 9 is cross-film mobility status of the embodiment when removing cryoprotective agent
Figure 10 is that embodiment cell when removing cryoprotective agent hangs cryoprotective agent concentration distribution in side runner
In figure, 1 is cell side core piece, and 2 be displacement side core piece, and 3 be semi-permeable membrane, and 4 be both-sided adhesive film, 11 cell side cores
The fluid channel of piece, 21 be the fluid channel for replacing side core piece, and 111 be the exchange section of cell side core piece, 112 entering for cell side core piece
Mouth section, 113 be the outlet section of cell side core piece, and 211 be the exchange section for replacing side core piece, and 212 be the entrance for replacing side core piece,
213 be the outlet section for replacing side core piece.
Specific embodiment
The utility model is described further with reference to the accompanying drawings and detailed description.
Referring to Fig. 1, a kind of spiral jatharapanvartanasana cryoprotective agent processing micro-fluidic chip, including cell side core piece 1, displacement side
Chip 2 and semi-permeable membrane 3, cell side core piece 1 and displacement side core piece 2 are fitted on semi-permeable membrane 3, cell side core piece 1 and displacement side
It is adhesively fixed between chip 2 by double coated film 4.
Referring to fig. 2, fluid channel 11 is carved on cell side core piece 1, and fluid channel 11 includes entrance 112,111 and of exchange section
Outlet section 113, width of flow path are Wc=0.8 millimeters, and depth is Hc=0.5 millimeters；The exchange section 111 of fluid channel 11 is spiral shell
Cycle rotation structure is made of multiple connected and tangent semi arches, is divided into A group and B group；A group includes 3 layers of concentric semi arch,
First layer semicircle arc radius is R1=10 millimeters, and second layer semicircle arc radius is R2=30 millimeters, third layer semicircle arc radius is
R3=50 millimeters；B group equally includes 3 layers of concentric semi arch, and first layer semicircle arc radius is R1, and second layer semicircle arc radius is
R2, third layer semicircle arc radius are R3；The distance of center circle of A group semi arch and B group semi arch is 2 times of R1；A group first layer semi arch one
End is connected with B group first layer semi arch one end, and the other end is connected with the second layer semi arch of B group；A group second layer semi arch one end
It is connected with B group first layer semi arch one end, the other end is connected with the third layer semi arch of B group；A group third layer semi arch one end and
B group second layer semi arch one end is connected, and the other end is connected with the axis of entrance 112；Every three layers of the other end of B group and outlet section
113 axis is connected.
It referring to Fig. 3, replaces and is carved with fluid channel 21 on side core piece 2, and fluid channel 21 includes entrance 212,211 and of exchange section
Outlet section 213, width of flow path are Wc, and depth is Hc；The exchange section 211 of fluid channel 21 is spiral revolving structure, topology
The exchange section 111 of structure snd size and the fluid channel 11 of cell side core piece 1 is consistent, is arranged symmetrically in 3 two sides of semi-permeable membrane.
Referring to fig. 4, semi-permeable membrane 3 is spiral same as the exchange section 211 of exchange section 111 or fluid channel 21 of fluid channel 11
Revolving structure, Wm=5 millimeters of width, with a thickness of 0.15 millimeter, material is polyether sulfone, and average pore size is 0.22 micron.
The entrance 112 of cell side core piece 1 is connected to cell suspending liquid entrance, and 1 outlet section 113 of cell side core piece is connected to cell
Suspension outlet, the entrance 212 of displacement side core piece 2 are connected to displacement liquid entrance, and the outlet section 213 of displacement side core piece is connected to displacement
The outlet of liquid.
Stem cell cryo-conservation is often dimethyl sulfoxide (DMSO), concentration 10% with cryoprotective agent.In order to cell
DMSO is added in suspension to this aimed concn, following setting can be used: being used DMSO concentration to be used as 20% solution and is set
Liquid is changed, and the initial concentration of DMSO is 0% in fresh cells suspension；It is micro- that the spiral jatharapanvartanasana cryoprotective agent processing is set
The cell suspending liquid inlet flow rate of fluidic chip is 3ml/min, the opening releases of cell suspending liquid；The inlet flow rate of displacement liquid is
3ml/min, the rate of discharge of displacement liquid are 0.
With this condition, the fluid channel 11 that the pressure in the fluid channel 21 of side core piece 2 is consistently greater than cell side core piece 1 is replaced
The pressure of corresponding position, as shown in Figure 5.Under transmembrane pressure driving, displacement liquid continues through cross-film and flows into cell suspension
Liquid, i.e. cross-film flow rate are always positive value, as shown in Figure 6.Concentration distribution such as Fig. 7 of DMSO in the fluid channel 11 of cell side core piece 1
It is shown, it is seen that rise from entrance (relative position 0) to outlet (relative position 1) DMSO concentration in continuous, uniform, therefore
It is expected that cell osmotic pressure experienced variation during add cryoprotective agent is steady, the permeability of cell damage compared with
It is small.In exit, cryoprotective agent concentration reaches 10%, realizes adding purpose.
After cryo-conservation, the cryoprotective agent in following setting removal cell suspending liquid can be used: using DMSO concentration
For 0% solution as displacement liquid, and in cell suspending liquid DMSO initial concentration be 10%；It is low that the spiral jatharapanvartanasana is set
The cell suspending liquid inlet flow rate of warm protective agent processing micro-fluidic chip is 3ml/min, the opening releases of cell suspending liquid；Displacement
The inlet flow rate of liquid is 18ml/min, and the rate of discharge of displacement liquid is 18ml/min.
With this condition, close to cell suspending liquid inlet side (relative position 0~0.5), the fluid channel of cell side core piece 1
Pressure is greater than the fluid channel corresponding position pressure of displacement side core piece 2 in 11, and (opposite close to cell suspending liquid outlet side
Position 0.5~1), pressure is less than the fluid channel corresponding position pressure of displacement side core piece 2 in the fluid channel 11 of cell side core piece 1,
As shown in Figure 8.Under transmembrane pressure driving, pass through in the extracellular solution in part close to cell suspending liquid inlet side cell suspending liquid
Cross-film flows into displacement liquid, i.e. cross-film flow rate is negative, and cell suspending liquid is concentrated；A side is being exported close to cell suspending liquid
It changes liquid and cell suspending liquid is flowed by cross-film, is i.e. cross-film flow rate is positive, and cell suspending liquid is diluted, as shown in Figure 9.At this
Under lasting " concentration-dilution " effect of kind, the concentration distribution of DMSO is as shown in Figure 10 in the fluid channel 11 of cell side core piece 1.It can
See from entrance (relative position 0) to outlet (relative position 1), DMSO concentration is in continuous, uniform decline, not no concentration
Mutation, therefore it is desired that cell osmotic pressure variation experienced during removing cryoprotective agent is steady, the infiltration of cell
Property damage it is smaller.In exit, the concentration of DMSO is reduced to 1% hereinafter, reaching removal cryoprotective agent purpose.Cell suspending liquid
Rate of discharge be 3ml/min, can match infusion require, thus realize carried out while to patient's infused cells suspension
Remove cryoprotective agent.
Particular embodiments described above has carried out into one the purpose of this utility model, technical scheme and beneficial effects
Step is described in detail, it should be understood that being not limited to this foregoing is merely specific embodiment of the utility model
Utility model, within the spirit and principle of the utility model, any modification, equivalent substitution, improvement and etc. done should all wrap
Containing being within the protection scope of the utility model.
Claims (6)
1. a kind of spiral jatharapanvartanasana runner cryoprotective agent handles micro-fluid chip, it is characterised in that: the spiral jatharapanvartanasana stream
Road cryoprotective agent handles micro-fluid chip, including cell side core piece, semi-permeable membrane and displacement side core piece, cell side core piece and displacement
Side core piece is fitted on semi-permeable membrane；Cell side core piece and displacement side core on piece have fluid channel, and fluid channel includes entrance
Section, exchange section and outlet section；Cell side core piece is shape identical spiral revolution with the exchange section of displacement side core on piece fluid channel
Structure is arranged symmetrically in semi-permeable membrane two sides；Semi-permeable membrane is same spiral revolving structure, and width is greater than cell side core piece and sets
Width of flow path on side change chip；The entrance of cell side core piece is connected to cell suspending liquid entrance, and cell side core piece outlet section connects
Logical cell suspending liquid outlet, the entrance of displacement side core piece are connected to displacement liquid entrance, and the outlet section of displacement side core piece is connected to displacement
The outlet of liquid；When micro-fluid chip is handled for cryoprotective agent, cell suspending liquid and displacement liquid are in semi-permeable membrane two sides miniflow
Reverse flow in road forms continuous transmembrane pressure in semi-permeable membrane two sides, and driving solution passes through semi-transparent membrane flow；When cell side core piece
Pressure is greater than pressure in displacement side core piece fluid channel in fluid channel, and the extracellular solution of cell suspending liquid is flowed to displacement liquid, cell
It is retained by semi-permeable membrane, cell suspending liquid is concentrated；When pressure is less than in displacement side core piece fluid channel in cell side core piece fluid channel
Pressure, displacement liquid are flowed to cell suspending liquid, and cell suspending liquid is diluted；Under continuous concentration or diluting effect, realization pair
The control of cryoprotective agent concentration in cell suspending liquid.
2. spiral jatharapanvartanasana runner cryoprotective agent handles micro-fluid chip according to claim 1, it is characterised in that: described
The axis of spiral revolving structure is made of several connected and tangent semi arches, can be divided into A group and B group；All semi arches of A group are same
The heart, innermost layer, that is, first layer semicircle arc radius are R1, and radius differs 2 times of R1 between adjacent each layer；All semi arches of B group are same
The heart, innermost layer, that is, first layer semicircle arc radius is also R1, and the center of circle of distance of center circle A group semi arch is 2 times of R1；The first layer of A group
Semi arch one end connects the first layer semi arch of B group, and the other end connects the second layer semi arch of B group；The second layer semi arch of A group
One end connects the first layer semi arch of B group, and the other end connects the third layer semi arch of B group；Second layer semi arch one end of B group connects
The first layer semi arch of A group is connect, the other end connects the third layer semi arch of A group.
3. spiral jatharapanvartanasana runner cryoprotective agent handles micro-fluid chip according to claim 1, it is characterised in that: described
The entrance and outlet section of the fluid channel of cell side core piece and displacement side core piece are straight channel, the exchange section of axis and fluid channel
Axis is tangent；Outlet section of the entrance of cell side core piece fluid channel close to displacement side core piece, the outlet section patch of cell side core piece
The entrance of nearly displacement side core piece.
4. spiral jatharapanvartanasana runner cryoprotective agent handles micro-fluid chip according to claim 1, it is characterised in that: described
Semi-permeable membrane is plane microfiltration membranes, and thickness is less than 200 microns, and aperture is less than 0.22 micron, and gabarit full-size is between 10~300 millis
Rice, material are mixed cellulose ester or polyether sulfone.
5. spiral jatharapanvartanasana runner cryoprotective agent handles micro-fluid chip according to claim 1, it is characterised in that: described
Cell side core piece or replace side core on piece fluid channel depth between 0.1 millimeter to 0.5 millimeter, width between 0.5 millimeter extremely
10 millimeters, length is between 100 millimeters to 1000 millimeters.
6. spiral jatharapanvartanasana runner cryoprotective agent handles micro-fluid chip according to claim 1, it is characterised in that: described
Cell side core piece, displacement side core piece and semi-permeable membrane are superimposed with sandwich structure, and semi-permeable membrane is in cell side core piece and displacement side core
Between piece, whole chip is assembled to form by macromolecule glue bonding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820913427.4U CN208354445U (en) | 2018-06-13 | 2018-06-13 | A kind of spiral jatharapanvartanasana runner cryoprotective agent processing micro-fluid chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820913427.4U CN208354445U (en) | 2018-06-13 | 2018-06-13 | A kind of spiral jatharapanvartanasana runner cryoprotective agent processing micro-fluid chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208354445U true CN208354445U (en) | 2019-01-11 |
Family
ID=64928753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820913427.4U Active CN208354445U (en) | 2018-06-13 | 2018-06-13 | A kind of spiral jatharapanvartanasana runner cryoprotective agent processing micro-fluid chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208354445U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109550530A (en) * | 2019-01-16 | 2019-04-02 | 承韶晖 | A kind of microfluid Dissolved Oxygen concentration Control chip |
-
2018
- 2018-06-13 CN CN201820913427.4U patent/CN208354445U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109550530A (en) * | 2019-01-16 | 2019-04-02 | 承韶晖 | A kind of microfluid Dissolved Oxygen concentration Control chip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105828848B (en) | blood oxygenator | |
| US11596901B2 (en) | Biomimetically designed modular microfluidic-based capillaries and lymphatic units for kidney and liver dialysis systems, organ bio-reactors and bio-artificial organ support systems | |
| ES2458140T3 (en) | MECS dialyzer | |
| JP4167058B2 (en) | Dual stage filtration cartridge | |
| EP2667908B1 (en) | De-airing oxygenator for treating blood in an extracorporeal blood circuit | |
| Wu et al. | Lung assist device: development of microfluidic oxygenators for preterm infants with respiratory failure | |
| CN106163584B (en) | Device for patient blood extracorporeal oxygenation | |
| Gaylor | Membrane oxygenators: current developments in design and application | |
| CN208354445U (en) | A kind of spiral jatharapanvartanasana runner cryoprotective agent processing micro-fluid chip | |
| EP1557185A1 (en) | Device for treating blood for extracorporeal circulation | |
| JP2019532916A5 (en) | ||
| CN102796662B (en) | Full-automatic cell processing apparatus and application thereof | |
| CN108684655A (en) | A kind of spiral jatharapanvartanasana runner cryoprotective agent processing micro-fluid chip | |
| CN103243026B (en) | Multifunctional full-automatic cell and solution treating instrument | |
| Zhou et al. | Theoretical and experimental study of a membrane-based microfluidics for loading and unloading of cryoprotective agents | |
| CN205667651U (en) | A kind of quick processing system of blood | |
| CN106659834B (en) | System for removing pro-inflammatory mediator and granulocyte and monocyte in blood | |
| CN204815042U (en) | Red blood cell washing device | |
| WO2022036738A1 (en) | Fluid treatment method and fluid treatment device | |
| CN209144171U (en) | Device for red blood cell load freeze drying protectant | |
| EP3600484B1 (en) | Oxygenator of organic fluids | |
| CN113462522B (en) | Deterministic lateral displacement micro-fluidic chip for separating magnetic beads from extracorporeal blood | |
| CN108949565A (en) | Device and method for red blood cell load freeze drying protectant | |
| CN220025600U (en) | Blood recovery transfusion bag for removing leucocyte | |
| CN215780394U (en) | Efficient and safe hemofiltration dialyzer and dialysis device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |