CN208350813U - Aureomycin hydrochloride Bacterial endotoxin test equipment - Google Patents

Abstract

The utility model relates to Bioexperiment fields, especially aureomycin hydrochloride Bacterial endotoxin test equipment, including a titer preparation facilities, test solution preparation facilities, reagents preparation device；The titer preparation facilities includes with the first fixed frame, in connected one first mixing chamber in the side of the first fixed frame, one first water filtration membrane being equipped in the top opening pipe of the first mixing chamber, an end cap is equipped on the top opening pipe of the first mixing chamber；Connection is equipped with a dissolution water supply pipe being connected with external feed end on the side wall of the open tube on the first water filtration membrane top, and the dissolution water supply pipe is equipped with dissolution flow valve.Rationally, checking procedure is simple, rapidly and accurately the content to aureomycin hydrochloride bacterial endotoxin can accurately be judged, more effectively meet the demand of people, solve problems of the prior art for the design of its device structure.

Description

Aureomycin hydrochloride Bacterial endotoxin test equipment

Technical field

The utility model relates to Bioexperiment fields, especially aureomycin hydrochloride Bacterial endotoxin test equipment.

Background technique

As a kind of therapeutic treatment drug, it is mainly used for treating conjunctivitis, the illnesss such as trachoma, therefore mesh aureomycin hydrochloride Preceding aureomycin hydrochloride largely be used to make the eye ointment of sterile preparation.It there has been in the execution standard of " Chinese Pharmacopoeia " to salt Sour aureomycin has authenticated statutory standards, but at present still without the check item to aureomycin hydrochloride bacterial endotoxin, mirror In aforementioned it is pointed it is present sterile preparation eye ointment will be usually made as when using aureomycin hydrochloride, therefore in order to guarantee medicine Therefore the quality of production of object must test to aureomycin hydrochloride bacterial endotoxin this parameter, carry out at present still without to it The method of inspection, it is clear that Bacterial endotoxin test standard of the existing aureomycin hydrochloride when the eye ointment as sterile ointment uses Can not more effectively meet the needs of people are to drug safety.

Utility model content

Aureomycin hydrochloride Bacterial endotoxin test equipment provided by the utility model, device structure design rationally, examine step It is rapid simple, can rapidly and accurately the content to aureomycin hydrochloride bacterial endotoxin is accurately judged, more effectively meet The demand of people, solves problems of the prior art.

Used technical solution is the utility model to solve above-mentioned technical problem: the inspection of aureomycin hydrochloride bacterial endotoxin Test equipment, including a titer preparation facilities, test solution preparation facilities, reagents preparation device；The titer preparation Device includes, in connected one first mixing chamber in the side of the first fixed frame, being opened at the top of the first mixing chamber with the first fixed frame It is equipped with one first water filtration membrane in mouth pipe, an end cap is equipped on the top opening pipe of the first mixing chamber；On the first water filtration membrane top Open tube side wall on connection be equipped with one and the dissolution water supply pipe that is connected of outside feed end, set on the dissolution water supply pipe There is dissolution flow valve, is equipped with one first lifting mixed plate, the top of the first lifting mixed plate in the first mixing chamber internal upper part Sealing is pierced by the first mixing chamber and is connected with one first lifting cylinder, first leads in the bottom interval of the first mixing chamber equipped with several Flow tube is respectively equipped with one first water conservancy diversion flow valve on each first diversion pipe；Cooperation is equipped with a standard below the first diversion pipe Liquid contains pipe；The side of titer preparation facilities is arranged in the test solution preparation facilities.

The titer preparation facilities includes one second fixed frame, in connected one second mixing in the side of the second fixed frame Room is equipped with one second water filtration membrane in the top opening pipe of the second mixing chamber, is equipped on the top opening pipe of the second mixing chamber One end cap；Connection is equipped with a Y type supply pipe being connected with external feed end on the side wall of the open tube on the second water filtration membrane top, It is equipped with branch pipe flow valve on two branch pipes of the Y type supply pipe, is equipped with one second lifting mixing in the second mixing chamber internal upper part Plate, the top seal of the second lifting mixed plate is pierced by the second mixing chamber and is connected with one second lifting cylinder, in the second mixing The bottom interval of room is equipped with several second diversion pipes, and one second water conservancy diversion flow valve is respectively equipped on each second diversion pipe；? Cooperation is equipped with a test solution and contains pipe below second diversion pipe.

The reagents preparation device includes a third fixed frame, in the connected third mixing in the side of third fixed frame Room is equipped with a third water filtration membrane in the top opening pipe of third mixing chamber, is equipped on the top opening pipe of third mixing chamber One end cap；Connection is equipped with a Y type supply pipe being connected with external feed end on the side wall of the open tube on third water filtration membrane top, It is equipped with branch pipe flow valve on two branch pipes of the Y type supply pipe, is equipped with third lifting mixing in third mixing chamber internal upper part Plate, the top seal of the third lifting mixed plate are pierced by third mixing chamber and are connected with a third lifting cylinder, mix in third The bottom interval of room is equipped with several third diversion pipes, and a third water conservancy diversion flow valve is respectively equipped on each third diversion pipe；? Cooperation is equipped with a reagents solution and contains pipe below third diversion pipe.

Distinguish in the top for each nozzle that each titer contains pipe, test solution contains pipe, reagents solution contains pipe Equipped with a water supply pipe, each water supply pipe is connected with a general pipeline respectively；The general pipeline is connected with the feed end.

It is respectively equipped with a disinfection cavity at the top of the first fixed frame, the second fixed frame, third fixed frame, is set in disinfection cavity There is a disinfection sponge ring, is equipped with sterile protective cover at the top of disinfection cavity.

Using the method for examining equipment to carry out aureomycin hydrochloride Bacterial endotoxin test, concrete operation step includes:

S1. the determination of endotoxin limit value；

S2. maximum valid dilution multiple is determined；

S3. the preparation of bacterial endotoxin standard solution is carried out by titer preparation facilities；

S4. endotoxic test solution is contained by the preparation of test solution preparation facilities；

S5. reagents solution is prepared by reagents preparation device；

S6. it is loaded；

S7. experimental result judges.

Aureomycin hydrochloride Bacterial endotoxin limit is arranged according to the rules in the step S1, the bacterial endotoxin of test sample Limit value L is 0.125EU/mg.

Bacterial endotoxin standard solution in the step S3, test solution, reagents solution are respectively provided in step S4 For multiple concentration gradients.

Beneficial effect possessed by the utility model is that device structure design is reasonable, by titer preparation facilities, for examination The quick preparation to various solution may be implemented in product solution-preparation device, reagents preparation device, so that the efficiency of experiment is improved, So that subsequent survey step is simple, it, can be rapidly and accurately to aureomycin hydrochloride bacterial endotoxin when carrying out solution preparation Content is accurately judged, more effectively meets the demand of people, solves problems of the prior art.

Detailed description of the invention

Fig. 1 is the connection relationship structural schematic diagram of the utility model.

In figure, 1, titer preparation facilities；2, test solution preparation facilities；3, reagents preparation device；4, first Mixing chamber；5, the first water filtration membrane；6, end cap；7, water supply pipe is dissolved；8, the first lifting mixed plate；9, the first lifting cylinder； 10, the first diversion pipe；11, the first water conservancy diversion flow valve；12, titer contains pipe；13, the second fixed frame；14, the second mixing chamber； 15, the second water filtration membrane；16, external feed end；17, Y type supply pipe；18, branch pipe flow valve；19, the second lifting mixed plate；20, Second lifting cylinder；21, the second diversion pipe；22, the second water conservancy diversion flow valve；23, test solution contains pipe；24, third is fixed Frame；25, third mixing chamber；26, third water filtration membrane；27, third goes up and down mixed plate；28, third lifting cylinder；29, third water conservancy diversion Pipe；30, third water conservancy diversion flow valve；31, reagents solution contains pipe；32, water supply pipe；33, general pipeline；34, disinfection cavity；35, it sterilizes Sponge ring；36, sterile protective cover；37, the first fixed frame.

Specific embodiment

In order to clarify the technical characteristics of the invention, below by specific embodiment, and its attached drawing is combined, to this reality It is described in detail with novel.

As shown in fig. 1, aureomycin hydrochloride Bacterial endotoxin test equipment, including a titer preparation facilities 1, test sample Solution-preparation device 2, reagents preparation device 3；The titer preparation facilities 1 includes being consolidated with the first fixed frame 37 first Connected one first mixing chamber 4 in side for determining frame 37, is equipped with one first water filtration membrane 5 in the top opening pipe of the first mixing chamber 4, Bacterial endotoxin national standard or working standard one are taken when preparing standard solution, flick bottle wall, powder is made to fall into bottom of bottle, Then with grinding wheel bottleneck top gently scratch then by contain bacterial endotoxin national standard vial carry out disinfection after Breakdown, because of breakdown during be more likely to produce chips of glass, after chips of glass is fallen on powder will with together enter it is molten Liquid is unfavorable for subsequent experiment effect, therefore powder effectively can be directly fallen in the first drainage after the first water filtration membrane 5 is arranged It is then dissolved again to the baterial endotoxin test that specified amount is added in dissolution water supply pipe 7 with water by external feed end 16 on film 5 Its content, in this way by dissolved liquid will by the first mixing chamber 4 after after enter directly into the first mixing chamber 4, And the glass asphalt being located on the first mixing chamber 4 cannot be introduced into, therefore can play good filtration, it is mixed first The top opening pipe for closing room 4 is equipped with an end cap 6, and end cap 6 is closed when needing to carry out solution mixing makes entire first mixing chamber 4 In environment in opposing seal, it is excessive during mixing to place solution；In the side of the open tube on 5 top of the first water filtration membrane Connection is equipped with a dissolution water supply pipe 7 being connected with external feed end 16 on wall, passes through the dissolution of external feed end 16 internally The baterial endotoxin test water of injection specified amount in water supply pipe 7, the dissolution water supply pipe 7 are equipped with dissolution flow valve, It can be very good control flow by dissolving flow valve, be equipped with one first lifting mixed plate 8, institute in 4 internal upper part of the first mixing chamber The top seal for stating the first lifting mixed plate 8 is pierced by the first mixing chamber 4 and is connected with one first lifting cylinder 9, when needing to first When liquid in mixing chamber 4 is mixed, needs to connect the first lifting cylinder 9 in the present apparatus with external power supply, then will Tracheae on first lifting cylinder 9 is connected with external control air pump, wherein the connection of the first lifting cylinder 9 and power supply uses It attacks concatenated mode directly, and upper switch is set on conducting wire, and the connection type of the first lifting cylinder 9 and air pump is straight using tracheae It connects in succession, connection type is simple, belongs to connection type known to those skilled in the art, is not belonging to the innovative point of the application, no It repeats again, the first mixing chamber 4 can quickly be driven by controlling the up and down motion of the first lifting cylinder 9 by control air pump when use The first interior lifting mixed plate 8 moves up and down, can be to the agriculture of its inside during the first lifting mixed plate 8 moves up and down Industry carries out mixing repeatedly, guarantees mixing quality and mixing efficiency, the bottom interval of the first mixing chamber 4 be equipped with several first Diversion pipe 10 is respectively equipped with one first water conservancy diversion flow valve 11, at this time the solution after mixing on each first diversion pipe 10 Concentration is relatively large at this time, need it is subsequent it is diluted to reach specified various concentration, in each first diversion pipe 10 Equipped with one first water conservancy diversion flow valve 11, control can be very good by the first water conservancy diversion flow valve 11 and flow out to each titer splendid attire The quantity of solution in pipe 12 show that different ratio needs to inject the molten of sterile aqueous to the inside of it according to calculating convenient for the later period Product, it is easy to operate convenient for control；Cooperation is equipped with a titer and contains pipe 12 below the first diversion pipe 10；The test sample is molten The side of titer preparation facilities 1 is arranged in liquid preparation facilities 2.

The titer preparation facilities 1 includes one second fixed frame 13, in the side of the second fixed frame 13 connected one second Mixing chamber 14 is equipped with one second water filtration membrane 15, at the top of the second mixing chamber 14 in the top opening pipe of the second mixing chamber 14 Open tube is equipped with an end cap 6；Connection is equipped with one and external feed end on the side wall of the open tube on 15 top of the second water filtration membrane 16 connected Y type supply pipes 17 are equipped with branch pipe flow valve 18 on two branch pipes of the Y type supply pipe 17, in the second mixing chamber 14 internal upper parts are equipped with one second lifting mixed plate 19, and the top seal of the second lifting mixed plate 19 is pierced by the second mixing chamber 14 It is connected with one second lifting cylinder 20, several second diversion pipes 21 is equipped in the bottom interval of the second mixing chamber 14, each the One second water conservancy diversion flow valve 22 is respectively equipped on two diversion pipes 21；Cooperation is equipped with a test solution below the second diversion pipe 21 Contain pipe 23；Baterial endotoxin test is added when preparing test solution to be dissolved with water, so just by dissolved liquid It can be by entering directly into the second mixing chamber 14 after after the second mixing chamber 14；Solution after mixing concentration at this time at this time It is relatively large, need it is subsequent it is diluted to reach specified various concentration, be designed with one in each second diversion pipe 21 Two water conservancy diversion flow valves 22 can be very good control by the second water conservancy diversion flow valve 22 and flow out to each test solution splendid attire pipe 23 The quantity of interior solution show that different ratio needs to inject the molten product of sterile aqueous to the inside of it according to calculating convenient for the later period, It is easy to operate convenient for control.

The reagents preparation device 3 includes a third fixed frame 24, in the connected third in the side of third fixed frame 24 Mixing chamber 25 is equipped with a third water filtration membrane 26, at the top of third mixing chamber 25 in the top opening pipe of third mixing chamber 25 Open tube is equipped with an end cap 6；Connection is equipped with one and external feed end on the side wall of the open tube on 26 top of third water filtration membrane 16 connected Y type supply pipes 17 are equipped with branch pipe flow valve 18 on two branch pipes of the Y type supply pipe 17, in third mixing chamber 25 internal upper parts are equipped with a third and go up and down mixed plate 27, and the top seal of the third lifting mixed plate 27 is pierced by third mixing chamber 25 It is connected with a third lifting cylinder 28, several third diversion pipes 29 is equipped in the bottom interval of third mixing chamber 25, each the A third water conservancy diversion flow valve 30 is respectively equipped on three diversion pipes 29；Cooperation is equipped with a reagents solution below third diversion pipe 29 Contain pipe 31；Take specification is several to meet defined reagents, the bottle wall for flicking each branch agent makes powder fall into bottom of bottle, then uses sand Wheel will be inserted directly into the disinfection sponge ring 35 for being soaked with thimerosal in bottleneck gently scratch at the scratch of body, be completed quick Disinfection, at this time by the way that the powder in bottle to be located on third water filtration membrane 26, at this time because of breakdown during be likely to produce Raw chips of glass will be unfavorable for subsequent experiment effect, therefore set with solution is entered together after chips of glass is fallen on powder Effectively powder can be directly fallen on third water filtration membrane 26 then again by external feed end 16 after setting third water filtration membrane 26 The inspection that specified amount is added to Y type supply pipe 17 dissolves its content with water, in this way will be by the by dissolved liquid It is entered directly into third mixing chamber 25 after after three mixing chambers 25, and the glass asphalt being located on third mixing chamber 25 can not Into, therefore good filtration can be played, pass through pneumatic third lifting cylinder 28 after solution enters in mixing chamber The third lifting mixed plate 27 in third mixing chamber 25 is driven to go up and down to stir solution, to complete to the uniform mixed of mixed liquor It closes.

The upper of each nozzle of pipe 31 is contained in each titer splendid attire pipe 12, test solution splendid attire pipe 23, reagents solution Side is respectively equipped with a water supply pipe 32, and each water supply pipe 32 is connected with a general pipeline 33 respectively；The general pipeline 33 and the feed end It is connected；It is contained by the feed end titer that can make suitable water successively at corresponding position that supplies water to water supply pipe 32 The solution that pipe 12, test solution contain pipe 23, reagents solution contains in pipe 31 is mixed, thus to reach various concentration The water yield of the difference of gradient, each water supplying pipe can be controlled by the switch valve of flowmeter thereon, it is easy to operate, point It does not control not individually, control flexibility ratio is high.

It is respectively equipped with a disinfection cavity 34 at the top of the first fixed frame 37, the second fixed frame 13, third fixed frame 24, is being disappeared It is equipped with a disinfection sponge ring 35 in malicious chamber 34, is equipped with sterile protective cover 36 at the top of disinfection cavity 34；By the way that disinfection cavity 34 is arranged The reagent body that can quickly open to needs carries out disinfection, and does not need the manual smearing every time to body progress repeatedly, Disinfection speed is fast, and effect is good, and wherein disinfection sponge ring 35 can be replaced regularly, and use is more convenient.

Wherein related definition and basic material are as follows in the present embodiment:

1. the Bacterial endotoxin limit (L) of drug, biological products generally determines as follows: L=K/M

L. is the Bacterial endotoxin limit of test sample in formula, is generally indicated with EU/ml, FU/mg or EU/U (active unit)；

K is the every kg body weight of people endotoxin dosage maximum acceptable per hour, with EU/ (kg.h), injection K=5EU/ (kg.h)；

M is people's every kg body weight maximum test sample dosage hourly, with m/ (kg.h), mg/ (kg.h) or U/ (kg.h)；

It is calculated with weight per capita by 60kg, body surface area is calculated by 1.62m2.

2. maximum valid dilution multiple refers to that in test test solution is allowed to reach diluted maximum multiple

(I → MVD) carries out the detection of endotoxin limit value under the concentration for being no more than this extension rate.

MVD:MVD=cL/ λ is determined with following formula, L is the Bacterial endotoxin limit of test sample in formula；

C is the concentration of test solution；

λ is the display sensitivity (EU/ml) of the reagents in gel method.

3. reagent

Table 1

Title: endotoxin working standard	Specification: 10EU
		Title: reagents	Sensitivity: 0.06EU/ml
Title: baterial endotoxin test water	Title: sodium hydroxide solution

The interference experiment of the method for aureomycin hydrochloride Bacterial endotoxin test is carried out, concrete operation step includes:

The washing of broken glass vessel, glassware is put into potassium chromate washing lotion or other pyrogen inactivators or cleaning solution sufficiently Impregnate, then take out and do washing lotion sky, thoroughly cleaned with tap water by washing lotion is remained, then with distilled water repeated flushing three times with On, it is put into suitable closed metal container after sky is dry or is placed into canister after being wrapped with masking foil, be put into high-temperature electric Hinder 1 furnace.

Resistance furnace is adjusted to 250 DEG C, starts timing after resistance furnace temperature rises to the temperature of setting, 250 DEG C dry 30 points roasting It is more than clock.After reaching the stipulated time, power cutoff, box temperature degree to be dried is down to room temperature naturally.In the feelings for not opening canister Under condition, it can be used in 2 days.

The determination of endotoxin limit value；Aureomycin hydrochloride Bacterial endotoxin limit is arranged according to the rules in the step S1, The Bacterial endotoxin limit L of test sample is 0.125EU/mg.

Determine maximum valid dilution multiple；The sensitivity of reagents used in this experiment is 0.06EU/ml, therefore can be obtained The diluted concentration of test sample is 0.25mg/ml out；

The preparation of bacterial endotoxin standard solution is carried out by titer preparation facilities 1；Take bacterial endotoxin national standard Product or working standard one, flick bottle wall, powder are made to fall into bottom of bottle, then with grinding wheel in bottleneck top gently scratch, by bottle It is placed at body scratch in disinfection sponge ring 35 and is sterilized after rotation, breakdown body falls in powder on the first filter membrane, because opening It is more likely to produce chips of glass during opening, will be unfavorable for solution is entered together after chips of glass is fallen on powder Subsequent experiment effect, therefore can effectively be directly fallen in powder on the first water filtration membrane 5 then after the first water filtration membrane 5 is set Its content is dissolved with water to the baterial endotoxin test that specified amount is added in dissolution water supply pipe 7 by external feed end 16 again, In this way by dissolved liquid will by the first mixing chamber 4 after after enter directly into the first mixing chamber 4, and be located at first Glass asphalt on mixing chamber 4 but cannot be introduced into, therefore can play good filtration；Closing end cap 6 makes whole at this time A first mixing chamber 4 is in the environment of opposing seal, and placement solution is excessive during mixing, passes through external feed end 16 The baterial endotoxin test water of injection specified amount, the dissolution water supply pipe 7 are equipped in dissolution water supply pipe 7 internally Flow valve is dissolved, can be very good control flow by dissolving flow valve；Concentration is relatively at this time for solution after mixing Greatly, need it is subsequent it is diluted to reach specified various concentration, be designed with one first water conservancy diversion in each first diversion pipe 10 Flow valve 11 can be very good the solution that control flows out in each titer splendid attire pipe 12 by the first water conservancy diversion flow valve 11 Quantity show that different ratio needs to inject the molten product of sterile aqueous to the inside of it according to calculating convenient for the later period, convenient for control, behaviour Facilitate；It is prepared into the bacterial endotoxin standard solution of 4 concentration, i.e. (λ is the mark of reagents used by 2 λ, 1 λ, 0.5 λ, 0.25 λ Show sensitivity), the solution after the completion of diluting is placed on external eddy mixer and mixes 30 seconds, guarantees adequately mixing.

Contain endotoxic test solution by the preparation of test solution preparation facilities 2；Take a bacterial endotoxin standard Product are added suitable baterial endotoxin test and are dissolved with water, will pass through the second mixing chamber by dissolved liquid in this way It is entered directly into after after 14 in second mixing chamber 14；Concentration is relatively large at this time for the solution after mixing at this time, after needing It is continuous it to be diluted to reach specified various concentration, it is designed with one second water conservancy diversion flow valve 22 in each second diversion pipe 21, It can be very good the quantity that control flows out to the solution that each test solution contains in pipe 23 by the second water conservancy diversion flow valve 22, Bacterial endotoxin standard items are successively diluted to 2 λ, l λ, 0.5 λ, 0.25 λ using the test solution that concentration is 025mg after mixing 4 concentration.

Reagents solution is prepared by reagents preparation device 3；The reagents for taking several standard specifications, flicking bottle wall makes Powder falls into bottom of bottle, and with grinding wheel, breakdown is spare after the bottleneck gently wiping of scratch ethyl alcohol cotton balls, disappears being placed at body scratch Sterilized after rotation in malicious sponge ring 35, breakdown body falls in powder on third filter membrane, because of breakdown during very may be used Chips of glass can be generated, subsequent experiment effect will be unfavorable for solution is entered together after chips of glass is fallen on powder, Therefore effectively powder can be directly fallen in after setting third water filtration membrane 26 and is then supplied again by external on third water filtration membrane 26 16 should be held to dissolve its content with water to the baterial endotoxin test that specified amount is added in dissolution water supply pipe 7, in this way by dissolution Liquid afterwards will be located on third mixing chamber 25 by entering directly into third mixing chamber 25 after after third mixing chamber 25 Glass asphalt but cannot be introduced into, therefore good filtration can be played；

Sample-adding；By ready reagents take wherein 18 be placed on rack for test tube, line up 5 column, 4 column 4,1 column 2；Its In 44 column each columns every addition 0.lm 2A, 1A, 0.5 enter, the bacterial endotoxin standard solution of 0.25 λ；Another column 2 add Entering 0.1ml inspection uses water as negative control.

Other 18 reagents are placed on rack for test tube, 5 column, 4 column 4,1 column 2 are lined up；44 column each columns therein are every Branch is separately added into 0.1m] the endotoxic test solution containing 2A, 1 λ, 0.5 λ, 0.25X, 2 addition 0.1m1 of another column are for examination Product solution is compareed as Sample Negative.

It after sample-adding, is sealed with sealed membrane, gently vibration mixes, and keeps away rabbit and generates bubble, is put into 37 DEG C together with rack for test tube In ± 1 DEG C of thermostat, after keeping the temperature (60+2) min, result is observed and recorded.

Calculating such as two groups of maximum concentration 2A is the positive, and 0.25 λ of minimum concentration is feminine gender, negative for being according to 4 pipes When negative, the geometrical mean (E2) of the reaction end concentration of the endotoxin standard solution made of inspection Check water is calculated as follows With the geometrical mean (Es) of the reaction end concentration of the endotoxin solution made of test solution or dilution.Es=1g^-1 (∑X/4)；Et=1g^-1(∑ Xt/4), Xs, Xt are respectively endogenous toxic material made of inspection water and test solution or dilution in formula The logarithm (lg) of the reaction end concentration of plain solution

Experimental result judgement:

When Es is at the λ of 0.5 λ ~ 2 (including 0.5 λ and 2 enters), and Et, at 0.5 λ -2 λ (including 0.5 λ and 2 λ), then it is assumed that Test sample not interference test under the concentration can carry out baterial endotoxin test to this test sample under the concentration.

Above-mentioned specific embodiment cannot function as the limitation to scope of protection of the utility model, for the skill of the art For art personnel, any alternate modification or transformation made to the utility model embodiment all fall within the guarantor of the utility model It protects in range.

Place is not described in detail for the utility model, is the well-known technique of those skilled in the art of the present technique.

Claims (5)

1. aureomycin hydrochloride Bacterial endotoxin test equipment, it is characterised in that: including a titer preparation facilities, test solution Preparation facilities, reagents preparation device；The titer preparation facilities includes with the first fixed frame, in the side of the first fixed frame Be connected one first mixing chamber, one first water filtration membrane is equipped in the top opening pipe of the first mixing chamber, on the top of the first mixing chamber Portion's open tube is equipped with an end cap；Connection is equipped with one and external feed end phase on the side wall of the open tube on the first water filtration membrane top Dissolution water supply pipe even, the dissolution water supply pipe are equipped with dissolution flow valve, in the first mixing chamber internal upper part equipped with one the One lifting mixed plate, the top seal of the first lifting mixed plate are pierced by the first mixing chamber and are connected with one first lifting cylinder, Several first diversion pipes are equipped in the bottom interval of the first mixing chamber, one first water conservancy diversion is respectively equipped on each first diversion pipe Flow valve；Cooperation is equipped with a titer and contains pipe below the first diversion pipe；The test solution preparation facilities setting is being marked The side of quasi- liquid preparation facilities.

2. aureomycin hydrochloride Bacterial endotoxin test equipment according to claim 1, it is characterised in that: the titer system Standby device includes one second fixed frame, in connected one second mixing chamber in the side of the second fixed frame, at the top of the second mixing chamber It is equipped with one second water filtration membrane in open tube, an end cap is equipped on the top opening pipe of the second mixing chamber；On the second water filtration membrane Connection is equipped with a Y type supply pipe being connected with external feed end, two branch pipes of the Y type supply pipe on the side wall of the open tube in portion On be equipped with branch pipe flow valve, the second mixing chamber internal upper part be equipped with one second lifting mixed plate, it is described second lifting mixed plate Top seal be pierced by the second mixing chamber and be connected with one second lifting cylinder, be equipped with several in the bottom interval of the second mixing chamber Second diversion pipe is respectively equipped with one second water conservancy diversion flow valve on each second diversion pipe；Cooperate below the second diversion pipe and is equipped with One test solution contains pipe.

3. aureomycin hydrochloride Bacterial endotoxin test equipment according to claim 2, it is characterised in that: the reagents is quasi- Standby device includes a third fixed frame, in the connected third mixing chamber in the side of third fixed frame, at the top of third mixing chamber It is equipped with a third water filtration membrane in open tube, an end cap is equipped on the top opening pipe of third mixing chamber；On third water filtration membrane Connection is equipped with a Y type supply pipe being connected with external feed end, two branch pipes of the Y type supply pipe on the side wall of the open tube in portion On be equipped with branch pipe flow valve, be equipped with a third in third mixing chamber internal upper part and go up and down mixed plate, the third goes up and down mixed plate Top seal be pierced by third mixing chamber and be connected with a third lifting cylinder, be equipped with several in the bottom interval of third mixing chamber Third diversion pipe is respectively equipped with a third water conservancy diversion flow valve on each third diversion pipe；Cooperate below third diversion pipe and is equipped with One reagents solution contains pipe.

4. aureomycin hydrochloride Bacterial endotoxin test equipment according to claim 3, it is characterised in that: contained in each titer Tubulature, test solution, which contain, manages, is respectively equipped with a water supply pipe, each benefit above each nozzle of reagents solution splendid attire pipe Water pipe is connected with a general pipeline respectively；The general pipeline is connected with the feed end.

5. aureomycin hydrochloride Bacterial endotoxin test equipment according to claim 4, it is characterised in that: fixed first Frame, the second fixed frame are respectively equipped with a disinfection cavity at the top of third fixed frame, and a disinfection sponge ring is equipped in disinfection cavity, The top of disinfection cavity is equipped with sterile protective cover.