CN208266194U - A kind of separation device for adipose-derived stem cells avoiding cross-infection - Google Patents

Abstract

The utility model discloses a kind of separation device for adipose-derived stem cells for avoiding cross-infection, including shell, the right side of the shell is equipped with control switch, it is closed by liner into hollow flushing chamber the inner cavity upper end of the shell, the upper surface material mouth of the shell is equipped with cock, separation hood is fixed in the middle part of the liner, the medial surface of the separation hood is equipped with filter membrane, the upper surface of the separation hood is evenly distributed with the sealing bar of vertical direction, the first electric telescopic rod of vertical direction is fixed in the mounting hole of the upper shell Middle face, originally the separation device for adipose-derived stem cells of cross-infection is avoided, structure is simple, the flushing and shake culture of fat stem cell can be completed at the same time, the fat stem cell of extraction can well realize final separation, mechanical automation replaces manual operations, control is stablized, fat stem cell is reduced simultaneously Damage, improve the survival rate of fat stem cell, it is easy to use.

Description

A kind of separation device for adipose-derived stem cells avoiding cross-infection

Technical field

The utility model relates to medical device technical field, specially a kind of fat stem cell for avoiding cross-infection is separated Device.

Background technique

Stem cell refers to the initial cell with self-renewing and multi-lineage potential, is the cells of origin of body, in spy The cell as one or more composition tissues or organ can be broken up under conditions of fixed, self-renewing refers to that stem cell can The size of stem cell population is maintained by cell division, Multidirectional Differentiation refers to that stem cell has plasticity, in different environments, Different histocytes can be divided into constitute the histoorgan of the various complexity of body, fat stem cell is in recent years from rouge A kind of isolated stem cell with multi-lineage potential, can be divided into skeletonization under specific inductive condition in fat tissue Cell, chondroblast and fat cell etc., due to extract stem cell equipment its structure is complicated, equipment weight, volume is big, price The limitation of high condition, it is only a small amount of at present to be applied to clinic, while the shear history of fat cell is manually to carry out, and be will cause big The damage for measuring cell, reduces the survival rate of stem cell, causes waste to blood, in addition, sampling during the separation process very Difficulty, trivial operations influence to separate, and are also easy to make stem cell by cross contamination, the limitation of application is larger, inconvenient for use.

Utility model content

The technical problems to be solved in the utility model is to overcome existing defect, provides a kind of fat for avoiding cross-infection Stem cell separator, structure is simple, can be completed at the same time flushing, the shake culture of fat stem cell, the fat stem cell of extraction It can well realize final separation, pollution-free, survival rate is high, and it is easy to use, it can effectively solve the problems in background technique.

To achieve the above object, the utility model provides the following technical solutions: a kind of fat avoiding cross-infection is dry thin Born of the same parents' separator, including shell, the right side of the shell are equipped with control switch, and the inner cavity upper end of the shell passes through liner Hollow flushing chamber is closed into, the upper surface material mouth of the shell is equipped with cock, is fixed with separation hood in the middle part of the liner, The medial surface of the separation hood is equipped with filter membrane, and the upper surface of the separation hood is evenly distributed with the sealing bar of vertical direction, described The first electric telescopic rod of vertical direction is fixed in the mounting hole of upper shell Middle face, under first electric telescopic rod End face is fixed with sealing plate, and the sealing bar passes through the axis hole in the middle part of sealing plate, is embedded in the liner on the upside of the separation hood Endless tube, the medial surface of the endless tube are evenly distributed with the jet pipe communicated with flushing chamber, and the right side of the liner is equipped with PBS tank-washer, The outlet of the PBS tank-washer is equipped with the delivery pipe communicated with endless tube, is equipped with the first drainage pump in the middle part of the delivery pipe, described Partition is fixed in the middle part of shell on the downside of liner, the upper surface outer ledge position of the partition is equipped with heater, the shell Lower surface in the middle part of be fixed with decelerating motor, shaft, the upper end of the shaft and deceleration are equipped in the axis hole in the middle part of the partition The output shaft of motor is fixedly linked, and the shaft side on the upside of the partition, which is radially distributed with, mixes axis, the shaft on the downside of the partition Side is radially distributed with impeller, and the shell inner cavity on the downside of the partition is equipped with piston plate, passes through in piston plate in the middle part of the shaft Portion's axis hole, the lower end surface of the shell are fixed with the second electric telescopic rod of vertical direction, second electric telescopic rod it is upper The lower end surface of end telescopic arm and piston plate is fixedly linked, and the upper surface of the piston plate is equipped with groove, is equipped in the groove Filter bowl, the filter mantle are the housing of upper end opening, are equipped with strainer in the middle part of the filter mantle, the filter mantle lower surface goes out Mouth is equipped with efferent duct, and the lower end surface of shell is run through in the lower end of the efferent duct, and the lower end of the delivery pipe is equipped with control valve, Supernatant liquid pipe and filler pipe are equipped in the middle part of shell on the downside of the partition, the middle part of the supernatant liquid pipe is equipped with the second drainage pump, The input terminal of the control switch is electrically connected with the output end of external power supply, and the output end of the control switch is electric with first respectively The input terminal electrical connection of dynamic telescopic rod, the first drainage pump, heater, decelerating motor, the second electric telescopic rod and the second drainage pump.

As a kind of optimal technical scheme of the utility model, liner left side position corresponding with filter membrane, which is equipped with, is put Slot is set, the side of the filter membrane is fixed with fixed ring, and the side of the fixed ring is fixed with the inserted link across placing groove.

As a kind of optimal technical scheme of the utility model, the side of the separation hood is open, and the left side of separation hood Face is equipped with putting hole, and the upper surface of the separation hood is evenly distributed with through-hole.

As a kind of optimal technical scheme of the utility model, the side of the shell is equipped with oxygen generator, described The input terminal of oxygen generator is electrically connected with the output end of control switch, and the outlet of the oxygen generator is equipped with and separation hood The oxygen pipe that the liner inner cavity of downside communicates.

As a kind of optimal technical scheme of the utility model, the strainer is in three layers of genesis analysis in filter mantle, and The mesh density of strainer is 200 mesh.

As a kind of optimal technical scheme of the utility model, shell medial surface is fixed between the partition and piston plate Strut, the inner end of the strut are fixed with the overflow plate of annular.

As a kind of optimal technical scheme of the utility model, shaft, institute are equipped in the axis hole of the heater medial surface The inner end for stating shaft is fixed with spoiler.

Compared with prior art, the utility model has the beneficial effects that

1) separation device for adipose-derived stem cells of cross-infection is originally avoided, structure is simple, can be completed at the same time fat stem cell It rinses and shake culture, the fat stem cell of extraction can well realize that final separation, mechanical automation replace manual operations, Control is stablized, while reducing the damage of fat stem cell, improves the survival rate of fat stem cell, easy to use；

2) it is rinsed by adipose tissue of the PBS solution in PBS tank-washer to extraction, it is ensured that fat stem cell Osmotic balance carries out the weak pressure swash of wave, uniformity from side by jet pipe to improve the stability of fat stem cell structure It is good, quick permeation；

3) separation that PBS solution and unstable material may be implemented by filter membrane is conducive to subsequent fat stem cell training The efficiency educated, filter membrane can be replaced by placing groove, easy to install and use；

4) mixing axis swing causes the dynamic of fat stem cell fluid to flow, and fat stem cell fluid can be achieved in matching disturbance flow piece Vibration, controlled by environment temperature of the heater to fat stem cell, cultivate fat stem cell, improve live Property, in order to subsequent separation, reduce influence of the separation damage to fat stem cell；

5) fat stem cell is stirred and is centrifugated by impeller, piston plate can rise, convenient for fat is dry The suspension fat on cell tissue liquid upper layer removes, and the tissue after supernatant and separation passes through supernatant liquid pipe and efferent duct row respectively Out, convenient separation, it is high-efficient.

Detailed description of the invention

FIG. 1 is a schematic structural view of the utility model.

In figure: 1 shell, 101 placing grooves, 2 control switches, 3 liners, 4 cocks, 5 separation hoods, 6 filter membranes, 601 fixed rings, 602 inserted links, 7 sealing bars, 8 first electric telescopic rods, 9 sealing plates, 10 endless tubes, 11 jet pipes, 12PBS tank-washer, 13 delivery pipes, 14 Axis, 20 impeller, 21 piston plates, 22 second electronic is mixed in one drainage pump, 15 partitions, 16 heaters, 17 decelerating motors, 18 shafts, 19 Telescopic rod, 23 filter mantles, 24 strainers, 25 efferent ducts, 26 control valves, 27 oxygen generators, 28 oxygen pipes, 29 overflow plates, 30 supernatants Liquid pipe, 31 second drainage pumps, 32 filler pipes, 33 shafts, 34 spoilers, 35 struts.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.

Referring to Fig. 1, the utility model provides a kind of technical solution: a kind of fat stem cell avoiding cross-infection separates Device, including shell 1, the right side of shell 1 are equipped with control switch 2, and the inner cavity upper end of shell 1 is closed in by liner 3 The upper surface material mouth of empty flushing chamber, shell 1 is equipped with cock 4, and the middle part of liner 3 is fixed with separation hood 5, the side of separation hood 5 Face is open, and the left side of separation hood 5 is equipped with putting hole, and the upper surface of separation hood 5 is evenly distributed with through-hole, the side of shell 1 Oxygen generator 27 is installed, the input terminal of oxygen generator 27 is electrically connected with the output end of control switch 2, oxygen generator 27 Outlet be equipped with the oxygen pipe 28 communicated with 3 inner cavity of liner of the downside of separation hood 5, the medial surface of separation hood 5 is equipped with filter membrane 6, interior 3 left sides position corresponding with filter membrane 6 is served as a contrast equipped with placing groove 101, the side of filter membrane 6 is fixed with fixed ring 601, fixed ring 601 Side be fixed with the inserted link 602 across placing groove 101, the upper surface of separation hood 5 is evenly distributed with the sealing bar of vertical direction 7, the first electric telescopic rod 8 of vertical direction is fixed in the mounting hole in the middle part of 1 upper surface of shell, the first electric telescopic rod 8 Lower end surface is fixed with sealing plate 9, and sealing bar 7 passes through the axis hole at 9 middle part of sealing plate, is embedded with ring in the liner 3 of 5 upside of separation hood Pipe 10, the medial surface of endless tube 10 are evenly distributed with the jet pipe 11 communicated with flushing chamber, and the right side of liner 3 is equipped with PBS tank-washer 12, The outlet of PBS tank-washer 12 is equipped with the delivery pipe 13 communicated with endless tube 10, and the middle part of delivery pipe 13 is equipped with the first drainage pump 14, leads to The PBS solution crossed in PBS tank-washer 12 is rinsed the adipose tissue of extraction, it is ensured that the infiltration of fat stem cell flattens Weighing apparatus carries out the weak pressure swash of wave from side by jet pipe to improve the stability of fat stem cell structure, and uniformity is good, quick permeation, The separation that PBS solution and unstable material may be implemented by filter membrane 6 is conducive to the efficiency that subsequent fat stem cell is cultivated, Filter membrane 6 can be replaced by placing groove 101, easy to install and use, be fixed with partition 15 in the middle part of the shell 1 of 3 downside of liner, The upper surface outer ledge position of partition 15 is equipped with heater 16, is equipped with shaft 33, shaft in the axis hole of 16 medial surface of heater 33 inner end is fixed with spoiler 34, is fixed with decelerating motor 17 in the middle part of the lower surface of shell 1, in the axis hole at 15 middle part of partition Equipped with shaft 18, the upper end of shaft 18 and the output shaft of decelerating motor 17 are fixedly linked, the 18 side diameter of shaft of 15 upside of partition Axis 19 is mixed to being distributed with, the swing of axis 19 is mixed and the dynamic of fat stem cell fluid is caused to flow, matching disturbance flow piece 34 can realize fat The vibration of stem cell fluid is controlled by environment temperature of the heater 16 to fat stem cell, obtains fat stem cell It cultivates, improves activity, in order to subsequent separation, reduce influence of the separation damage to fat stem cell, 15 downside of partition turns 18 side of axis is radially distributed with impeller 20, and 1 inner cavity of shell of 15 downside of partition is equipped with piston plate 21, partition 15 and piston plate 21 it Between 1 medial surface of shell be fixed with strut 35, the inner end of strut 35 is fixed with the overflow plate 29 of annular, passes through and lives in the middle part of shaft 18 21 middle part axis hole of seben, the lower end surface of shell 1 are fixed with the second electric telescopic rod 22 of vertical direction, the second electric telescopic rod 22 Telescopic upper end arm and the lower end surface of piston plate 21 be fixedly linked, the upper surface of piston plate 21 is equipped with groove, is equipped in groove Filter bowl 23, filter mantle 23 are the housing of upper end opening, and the middle part of filter mantle 23 is equipped with strainer 24, and strainer 24 is in filter mantle 23 In three layers of genesis analysis, and the mesh density of strainer 24 is 200 mesh, and the outlet of 23 lower surface of filter mantle is equipped with efferent duct 25, defeated The lower end surface of shell 1 is run through in the lower end of outlet pipe 25, and the lower end of delivery pipe 25 is equipped with control valve 26, the shell 1 of 15 downside of partition Middle part is equipped with supernatant liquid pipe 30 and filler pipe 32, and the middle part of supernatant liquid pipe 30 is equipped with the second drainage pump 31, the input of control switch 2 End be electrically connected with the output end of external power supply, the output end of control switch 2 respectively with the first electric telescopic rod 8, the first drainage pump 14, the input terminal electrical connection of heater 16, decelerating motor 17, the second electric telescopic rod 22 and the second drainage pump 31, originally avoids handing over The separation device for adipose-derived stem cells of infection is pitched, structure is simple, can be completed at the same time the flushing and shake culture of fat stem cell, extracts Fat stem cell can well realize final separation, mechanical automation replaces manual operations, and control is stablized, while reducing rouge The damage of fat stem cell improves the survival rate of fat stem cell, easy to use, is stirred by impeller 20 fat stem cell Centrifuge separation is mixed and stirred, piston plate 21 can rise, convenient for the suspension fat on fat stem cell tissue fluid upper layer is removed, supernatant Pass through supernatant liquid pipe 30 respectively with the tissue after separation and efferent duct 25 is discharged, convenient separation is high-efficient.

When in use: opening cock 4, the flushing that the adipose tissue of extraction is placed in 9 upside of sealing plate is intracavitary, the first drainage 14 work of pump is sprayed into the PBS washing lotion in PBS tank-washer 12 by jet pipe 11 in the adipose tissue of the upside of sealing plate 9, the after flushing The work of one electric telescopic rod 8 mentions sealing plate 9 upwards, and adipose tissue liquid flows down and is filtered through filter membrane 6, filtered fat Tissue fluid temporal aggregate gives temperature appropriate and oxygen in the upside of partition 15, by heater 16 and oxygen generator 27, The of short duration culture for carrying out adipose tissue is mixed roller 19 and is rotated with shaft 18, and the deflection of matching disturbance flow piece 34 forms adipose tissue liquid Turbulent flow, the slow axis hole by 15 middle part of partition fall to the upside of piston plate 21,17 work belt moving vane 20 of decelerating motor with Shaft 18 rotates, to carry out the sirring and separating of adipose tissue liquid, the second electric telescopic rod 22 is upward by piston plate 21 after separation It pushes away, supernatant is discharged by supernatant liquid pipe 30, control valve 26 is opened, through the filtered fat stem cell of filter screen 24 by exporting Pipe 25 is discharged.

The utility model structure is simple, can be completed at the same time the flushing and shake culture of fat stem cell, and the fat of extraction is dry Cell can well realize final separation, avoid cross-infection, survival rate is high, easy to use.

While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (3)

1. a kind of separation device for adipose-derived stem cells for avoiding cross-infection, including shell (1), it is characterised in that: the shell (1) Right side be equipped with control switch (2), the inner cavity upper end of the shell (1) is closed by liner (3) into hollow flushing chamber, The upper surface material mouth of the shell (1) is equipped with cock (4), is fixed with separation hood (5) in the middle part of the liner (3), described point Medial surface from cover (5) is equipped with filter membrane (6), and the upper surface of the separation hood (5) is evenly distributed with the sealing bar of vertical direction (7), it is fixed with the first electric telescopic rod (8) of vertical direction in the mounting hole in the middle part of shell (1) upper surface, described first The lower end surface of electric telescopic rod (8) is fixed with sealing plate (9), and the sealing bar (7) passes through the axis hole in the middle part of sealing plate (9), institute It states and is embedded with endless tube (10) in the liner (3) on the upside of separation hood (5), the medial surface of the endless tube (10) is evenly distributed with and rinses The jet pipe (11) that chamber communicates, the right side of the liner (3) are equipped with PBS tank-washer (12), the outlet of the PBS tank-washer (12) be equipped with The delivery pipe (13) that endless tube (10) communicates is equipped with the first drainage pump (14) in the middle part of the delivery pipe (13).

2. a kind of separation device for adipose-derived stem cells for avoiding cross-infection according to claim 1, it is characterised in that: described Liner (3) left side position corresponding with filter membrane (6) is equipped with placing groove (101), and the side of the filter membrane (6) is fixed with fixed ring (601), the side of the fixed ring (601) is fixed with the inserted link (602) across placing groove (101).

3. a kind of separation device for adipose-derived stem cells for avoiding cross-infection according to claim 1, it is characterised in that: described The side of separation hood (5) is open, and the left side of separation hood (5) is equipped with putting hole, and the upper surface of the separation hood (5) is uniformly divided It is furnished with through-hole.