CN208239463U - Glycosylated hemoglobin detection device - Google Patents

Abstract

The utility model belongs to the field of medical instrument technology, disclose a kind of glycosylated hemoglobin detection device, including support plate, the workbench being placed in support plate, and the first panel and second panel of distribution support board ends, the support plate, first panel, second panel is enclosed the accommodating chamber for accommodating workbench, it is mounted on the table for sample feeding, pretreatment, washing, separation, it is detected on integrated micro-fluidic chip, motor for driving the micro-fluidic chip work is installed in first panel, switch is provided in second panel.In this way, by being mounted with micro-fluidic chip on the table, in the accommodation groove of micro-fluidic chip placement on the table, since micro-fluidic chip integrates sample feeding, sample pretreatment, sample washing, sample separation, sample detection, greatly simplifie operating process, sample and reagent consumption are reduced, is switched by setting, convenient for the simple and convenient starting or closing for controlling the device.

Description

Glycosylated hemoglobin detection device

Technical field

The utility model belongs to the field of medical instrument technology, is to be related to a kind of glycosylated hemoglobin detection more specifically Device.

Background technique

Diabetes are the incretion metabolism diseases that one group of cause of disease and pathogenesis are not yet completely understood, have high concurrent disease and High lethality rate, the disease incidence of diabetes was on the rise in recent years, was to seriously threaten the worldwide of human health public to defend Raw problem.

Clinically diabetes are mainly determined by detection glycemic parameters, and glycemic parameters only represent blood glucose when drawing blood Level, to making a definite diagnosis with limitation.In normal human, there are three types of hemoglobins: HbA, HbF, HbA2, and in adult erythrocyte HbA is mainly contained, when separating hemoglobin with chromatography, 3 kinds of sugar content ingredients can be eluted i.e.: HbA1a, HbA1b, HbA1c is collectively referred to as glycosylated hemoglobin.In recent years, the detection of glycosylated hemoglobin is increasingly subject to clinical great attention, glucose Change that a part of hemoglobin that hemoglobin (HbA1C) refers to that in blood and glucose combines.The service life of red blood cell in human body Generally 120 days, before red blood cell is dead, glucose in blood hemoglobin (HbA1C) content was also remained relatively unchanged over, Glucosylation hemoglobin (HbA1C) horizontal reverse has answered the preceding 120 days average blood glucose levels of detection, and with take blood time, patient Whether whether on an empty stomach and unrelated using factors such as insulin, therefore, glucosylation hemoglobin (HbA1C) is reaction long-term blood glucose The important indicator of horizontal standard and diabetes auxiliary diagnosis, monitoring, treatment.

The measuring method of glucosylation hemoglobin (HbA1C) is varied, and be roughly divided into two classes: one kind is to be based on The charge of HbA1C and HbA (hemoglobin) is different, such as ion exchange chromatography；Another kind of is the knot based on the group that is saccharified on Hb Structure feature, such as affinity chromatography, these methods pass through large-scale Biochemical Analyzer or ion-exchange high-voltage liquid phase chromatogram instrument mostly Complete, sample consumption is big, operation sequence is complicated, instrument reagent is at high cost, therefore, exploitation quickly, intuitive, carrying side Just, become the emphasis currently studied both at home and abroad for the detection device of glycosylated hemoglobin easily to operate.

Utility model content

The purpose of this utility model is to provide a kind of glycosylated hemoglobin detection devices, it is intended to which solution is surveyed in the prior art Determine the high technical problem of the equipment of glycosylated hemoglobin.

To achieve the goals above, the technical solution adopted in the utility model is: glycosylated hemoglobin detection device, including Support plate, the workbench being placed in the support plate and the first panel for protruding extension respectively by the support board ends With second panel, the support plate, the first panel, the second panel are enclosed the appearance for accommodating the workbench Receive chamber, be mounted on the workbench for sample feeding, pretreatment, washing, separate, be detected on integrated micro-fluidic core Piece, and the accommodation groove for accommodating the micro-fluidic chip is offered on the workbench, it is installed in the first panel useful It is examined in being provided on the motor for driving the micro-fluidic chip work, the second panel for controlling the glycosylated hemoglobin The switch surveying device starting or closing.

Further, the micro-fluidic chip includes chip basal body, is set in the chip basal body and for for blood sample stream Logical sample intake passage, the sample inlet pool for entering the sample intake passage for blood sample, for described in the sample intake passage Blood sample carry out pretreated sample adsorption zone, for clean pretreatment after sample sample scrubbing section, for adsorbing blood sample impurity The sample collection area of hemoglobin is isolated with this, the blood red egg of glucosylation is isolated with this for being handled hemoglobin White buffer solution area and the sample detection pond for glucosylation content of hemoglobin in test sample, the sample inlet pool, The sample adsorption zone, the sample scrubbing section, the sample collection area, the sample detection pond are sequentially arranged in the chip base It is serially connected on body and by the stand in channel, the buffer solution area is set to the side in the sample detection pond and passes through feed liquor Channel is connected to the sample detection pond.

Further, the buffer solution area include set on sample detection pond side the first buffer solution pond, with And the second buffer solution pond set on the sample detection pond other side, first buffer solution pond and first buffering are molten Liquid pool passes through feed pathway respectively and is connected to the sample detection pond.

Further, the sample inlet pool, the sample adsorption zone, the sample scrubbing section, the sample collection area and The sample detection pond is distributed in the chip basal body in ring-like.

It further, further include for carrying out pretreated first electrode, the anode or yin of the first electrode to blood sample Pole is inserted into the sample inlet pool, and the cathode or anode of the first electrode are inserted into the sample detection pond.

It further, further include that the second of glucosylation hemoglobin is isolated with this for being handled hemoglobin The anode or cathode of electrode, the second electrode is inserted into first buffer solution pond, the cathode or sun of the second electrode Pole is inserted into second buffer solution pond.

Further, further include light shield layer for covering the sample intake passage Yu the sample adsorption zone.

It further, further include the wind being set to below the workbench and for cooling down to the micro-fluidic chip Fan.

Further, temperature sensor and the control for detecting the micro-control stream chip temperature are additionally provided on the workbench The pcb board of the fan work is made, the pcb board and the fan are electrically connected, the temperature sensor and pcb board electricity Property connection.

It further, further include being respectively used to fix the first panel, the second panel and the support plate Floor.

The beneficial effect of glycosylated hemoglobin detection device provided by the utility model is: compared with prior art, this The glycosylated hemoglobin detection device of utility model, by the micro-fluidic core for being mounted with detection glycosylated hemoglobin on the table Piece, in micro-fluidic chip placement accommodation groove on the table, due to micro-fluidic chip collection sample feeding, sample pretreatment, Sample washing, sample separation, sample detection greatly simplifie operating process in one, reduce sample and reagent consumption, and And without being equipped with expensive instrument, use cost is significantly reduced, makes it possible that scene is detected immediately, there is huge warp Ji value and social value, in addition, being switched by being arranged in second panel, so as to the simple and convenient control device Starting is closed.

Detailed description of the invention

It, below will be to embodiment or the prior art in order to illustrate more clearly of the technical scheme in the embodiment of the utility model Required practical attached drawing is briefly described in description, it should be apparent that, the accompanying drawings in the following description is only the present embodiment Some embodiments for those of ordinary skill in the art without creative efforts, can also basis These attached drawings obtain other attached drawings.

Fig. 1 is the main view for the glycosylated hemoglobin detection device that the utility model embodiment proposes；

Fig. 2 is the top view for the glycosylated hemoglobin detection device that the utility model embodiment proposes；

Fig. 3 is the schematic diagram figure for the microfluidic chip structure that the utility model embodiment proposes.

Wherein, each appended drawing reference in figure:

1- support plate；2- first panel；3- second panel；4- workbench；5- micro-fluidic chip；6- fan；7- switch；8- Motor；9- floor；10- first electrode；11- second electrode；51- chip basal body；52- sample inlet pool；53- sample adsorption zone；54- sample Product scrubbing section；55- sample collection area；56- sample detection pond, 57- buffer solution area；The first buffer solution of 571- pond；572- Two buffer solution ponds；58- sample intake passage；59- feed pathway.

Specific embodiment

In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation Example, the present invention will be further described in detail.It should be appreciated that specific embodiment described herein is only used to explain The utility model is not used to limit the utility model.

It should be noted that it can be directly another when element is referred to as " being fixed on " or " being set to " another element On one element or it may be simultaneously present centering elements.When an element is known as " being connected to " another element, it can be with It is directly to another element or may be simultaneously present centering elements.

In addition, it should also be noted that, positional terms such as left and right, upper and lower in the utility model embodiment, are only mutual Be relative concept or with the normal operating condition of product be reference, and should not be regarded as it is restrictive below in conjunction with The realization of the utility model is described in detail in specific embodiment.

As shown in Figures 1 to 3, the present embodiment proposes a kind of glycosylated hemoglobin detection device, including support plate 1, at this It is mounted with workbench 4 in support plate 1,1 both ends of support plate are provided with first panel 2 and second panel 3, and the first panel 2 extend with second panel 3 along far from 1 direction of support plate, which is mutually enclosed with the second panel 3 for holding The accommodating chamber (attached drawing is not made) for setting above-mentioned workbench, being mounted on above-mentioned workbench 4 can be used for sample feeding, pre- place It manages, wash, separating, being detected on integrated micro-fluidic chip 5, accommodation groove (attached drawing is not made) being offered on workbench 4, this is micro- Fluidic chip 5 is placed in accommodation groove, in addition, being equipped in above-mentioned first panel 2 for driving above-mentioned 5 work of micro-fluidic chip The motor 8 of work, be additionally provided in above-mentioned second panel 3 for control glycosylated hemoglobin detection device starting or close Switch 7.In this way, by the micro-fluidic chip 5 for being mounted with detection glycosylated hemoglobin on workbench 4, the micro-fluidic chip 5 peace It sets in the accommodation groove on workbench 4, since micro-fluidic chip 5 collects sample feeding, sample pretreatment, sample washing, sample point From, sample detection in one, operating process is greatly simplified, reduces sample and reagent consumption, and does not have to be equipped with expensive Instrument, significantly reduce use cost, make it possible live detection immediately, there is huge economic value and social valence Value.Preferably, above-mentioned first panel 2, second panel 3 are integrally formed with support plate 1 respectively, naturally it is also possible to pass through the side of screw thread Formula connection, above-mentioned glycosylated hemoglobin device can also be automatically controlled by system, not limited uniquely herein.

Further, referring to Fig. 3, one kind as glycosylated hemoglobin detection device provided by the utility model is specific Embodiment, above-mentioned micro-fluidic chip 5 include chip basal body 51, be successively arranged in chip basal body 51 sample inlet pool 52, for pair Blood sample carry out pretreated sample adsorption zone 53, for clean pretreatment after sample sample scrubbing section 54, for adsorbing blood Impurity with this isolates the sample collection area 55 of hemoglobin, isolates grape for being handled hemoglobin with this in sample The buffer solution area 57 of glycosylated hemoglobin and sample detection pond for glucosylation content of hemoglobin in test sample 56, it further include the sample intake passage 58 for circulating for blood sample in above-mentioned micro-fluidic chip 5, it will be above-mentioned by the realization of sample intake passage 58 Sample inlet pool 52, sample adsorption zone 53, sample scrubbing section 54, sample collection area 55, sample detection pond 56 serial connection, this Outside, above-mentioned buffer solution area 57 is set to the side in sample detection pond 56 and is connected by feed pathway 59 and sample detection pond 56 It is logical.In this way, passing through setting sample inlet pool 52, sample adsorption zone 53, sample scrubbing section 54, sample collection area 55, sample detection pond 56 In in chip basal body 51 so that the glycosylated hemoglobin detection device collection sample feeding, sample pretreatment, sample washing, Sample separation, sample detection greatly simplifie operating process in one, reduce sample and reagent consumption, and do not have to match Standby expensive instrument, significantly reduces use cost, makes it possible that scene is detected immediately.

Further, referring to Fig. 3, one kind as glycosylated hemoglobin detection device provided by the utility model is specific Embodiment, above-mentioned buffer solution area 57 include the first buffer solution pond 571 and the second buffer solution pond 572, and the first buffering is molten Liquid pool 571 and the second buffer solution pond 572 are located at the two sides in sample detection pond 56, and are examined by feed pathway 59 and sample Pond 56 is surveyed to be connected to.In the present embodiment, above-mentioned first buffer solution pond 571, sample detection pond 56, the second buffer solution pond 572 Along lineal layout, in this way, being convenient for the circulation of sample liquid, certainly, above-mentioned first buffer solution pond 571, sample detection pond 56, second Buffer solution pond 572 can also be distributed in camber, S type, not limited uniquely herein.

Further, referring to Fig. 3, one kind as glycosylated hemoglobin detection device provided by the utility model is specific Embodiment, above-mentioned sample inlet pool 52, sample adsorption zone 53, sample scrubbing section 54, sample collection area 55, sample detection pond 56 are in ring Shape is distributed in chip basal body 51, by being set to annular, so as to meet the behaviour carried out under different modes to fluid Control, such as using it is centrifugal to fluid running manipulated, pump valve system to fluid operate manipulate, certainly, in this implementation In example, above-mentioned sample inlet pool 52, sample adsorption zone 53, sample scrubbing section 54, sample collection area 55, sample detection pond 56 can also be along straight Line distribution, does not limit uniquely herein.

Further, referring to Fig. 3, one kind as glycosylated hemoglobin detection device provided by the utility model is specific Embodiment, above-mentioned glycosylated hemoglobin detection device further include for carrying out pretreated first electrode 10 to blood sample, this The anode or cathode of one electrode 10 is inserted into above-mentioned sample adsorption zone 53, and the cathode or anode of the first electrode 10 are inserted into above-mentioned sample In product detection cell 56, when in use, required antigen or antibody can be fixed in advance in sample adsorption zone, by blood to be measured when detection Final proof product are added in sample inlet pool 52, are then entered in sample adsorption zone under the stimulation of electric current by sample intake passage 58 and are immunized Then agglutinating reaction cleans pretreated sample by sample scrubbing section 54, using sample Nesting Zone 55 to inhale Impurity in attached blood sample isolates hemoglobin with this, finally, hemoglobin opens Photoelectric Detection after flowing into sample detection pond 56 Device is detected, so that micro-fluidic chip 5 collects sample feeding, sample pretreatment, sample washing, sample separation, sample inspection It surveys in one, greatly simplifies operating process, reduce sample and reagent consumption.

Further, referring to Fig. 3, one kind as glycosylated hemoglobin detection device provided by the utility model is specific Embodiment, above-mentioned micro-fluidic chip 5 further include isolating the blood red egg of glucosylation for being handled hemoglobin with this The anode or cathode of white second electrode 11, the second electrode 11 is inserted into above-mentioned first buffer solution pond 571, second electrode 11 Cathode or anode be inserted into the second buffer solution pond 572, since the first buffer solution pond 571, sample detection pond 56, second are slow Rush solution pool 572 and circulated by feed pathway 59, in this way, can according to the charge difference of various composition institute band in hemoglobin, To separate its hemoglobin, the precision of subsequent detection is improved.

Further, referring to Fig. 3, one kind as glycosylated hemoglobin detection device provided by the utility model is specific Embodiment, above-mentioned micro-fluidic chip 5 further include light shield layer (attached drawing is not made), the light shield layer can be used for by sample intake passage 58 with Sample adsorption zone 53 covers in, the sample intake passage 58 and the sample solution in sample adsorption zone 53 that can guarantee chip interior in this way With reaction product not by the interference of extraneous visible light and detection light source.Preferably, in the present embodiment, above-mentioned light shield layer is using black Color polymethyl methacrylate preparation, it is of course also possible to use other materials, do not limit uniquely herein.

Further, Fig. 1 and Fig. 2 are please referred to, one as glycosylated hemoglobin detection device provided by the utility model Kind specific embodiment, above-mentioned hemoglobin detection device further include the fan 6 for being installed on 4 lower section of workbench, pass through setting One fan 6 can be used for cooling down to above-mentioned micro-fluidic chip 5, in this way, working as micro-fluidic chip 5 at work, can remain The fluctuating change of temperature is little, so that the follow-up work for micro-fluidic chip 5 provides better guarantee.

Specifically, in the present embodiment, the temperature for detecting 5 temperature of micro-fluidic chip is additionally provided on above-mentioned workbench Sensor (attached drawing is not made) and pcb board (attached drawing is not made) for controlling fan work, the pcb board electrically connect with fan 6 It connects, the temperature sensor and pcb board are electrically connected, in this way, when temperature sensor detects that the temperature of micro-fluidic chip 5 is higher When, it is worked by pcb board driving fan 6, when the temperature of micro-fluidic chip 5 is fallen to a certain temperature, then temperature sensor can incite somebody to action Signal is transferred to pcb board, and pcb board can be disconnected with fan, so that fan be made to stop working.

Further, Fig. 1 and Fig. 2 are please referred to, one as glycosylated hemoglobin detection device provided by the utility model Kind of specific embodiment, above-mentioned glycosylated hemoglobin detection device further includes floor 9, the floor 9 can be used for by first panel 2, Second panel 3 and support plate 1 are fixed, to improve the bond strength between floor 9 and first panel 2, second panel 3.

The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this Made any modifications, equivalent replacements, and improvements etc., should be included in the utility model within the spirit and principle of utility model Protection scope within.

Claims (10)

1. glycosylated hemoglobin detection device, which is characterized in that including support plate, the workbench being placed in the support plate, And protrude the first panel and second panel of extension respectively by the support board ends, the support plate, the first panel, The second panel is enclosed the accommodating chamber for accommodating the workbench, be mounted on the workbench for sample into Sample pretreatment, washing, separates, is detected on integrated micro-fluidic chip, and offers on the workbench described micro- for accommodating The accommodation groove of fluidic chip, is equipped with the motor for driving the micro-fluidic chip work in the first panel, and described the It is provided on two panels for controlling switch glycosylated hemoglobin detection device starting or closed.

2. glycosylated hemoglobin detection device as described in claim 1, which is characterized in that the micro-fluidic chip includes chip Matrix, the sample intake passage in the chip basal body and for circulating for blood sample are used to enter the sample intake passage for blood sample Sample inlet pool, for carrying out pretreated sample adsorption zone, for cleaning pretreatment to the blood sample in the sample intake passage Afterwards the sample scrubbing section of sample, for adsorbing blood sample impurity the sample collection area of hemoglobin is isolated with this, for blood red Albumen, which is handled, isolates the buffer solution area of glucosylation hemoglobin with this and for glucosylation in test sample The sample detection pond of content of hemoglobin, the sample inlet pool, the sample adsorption zone, the sample scrubbing section, the sample are poly- Ji Qu, the sample detection pond are sequentially arranged in the chip basal body and are serially connected to by the sample intake passage, the buffering Solution area is set to the side in the sample detection pond and is connected to by feed pathway with the sample detection pond.

3. glycosylated hemoglobin detection device as claimed in claim 2, which is characterized in that the buffer solution area includes being set to First buffer solution pond of sample detection pond side and the second buffer solution set on the sample detection pond other side Pond, first buffer solution pond and first buffer solution pond pass through feed pathway respectively and the sample detection pond connects It is logical.

4. glycosylated hemoglobin detection device as claimed in claim 2, which is characterized in that the sample inlet pool, the sample are inhaled Attached area, the sample scrubbing section, the sample collection area and the sample detection pond are distributed in the chip basal body in ring-like On.

5. glycosylated hemoglobin detection device as claimed in claim 2, which is characterized in that further include pre- for being carried out to blood sample The first electrode of processing, the anode or cathode of the first electrode are inserted into the sample inlet pool, the cathode of the first electrode or Anode is inserted into the sample detection pond.

6. glycosylated hemoglobin detection device as claimed in claim 3, which is characterized in that further include for hemoglobin into The second electrode of glucosylation hemoglobin is isolated in row processing with this, the anode or cathode insertion of the second electrode described the In one buffer solution pond, in cathode or anode insertion second buffer solution pond of the second electrode.

7. glycosylated hemoglobin detection device as claimed in claim 2, which is characterized in that further include for covering the sample introduction The light shield layer in channel and the sample adsorption zone.

8. glycosylated hemoglobin detection device as described in claim 1, which is characterized in that further include being set to the workbench Lower section and the fan for being used to cool down to the micro-fluidic chip.

9. glycosylated hemoglobin detection device as claimed in claim 8, which is characterized in that be additionally provided with and be used on the workbench It detects the temperature sensor of the micro-fluidic chip temperature and controls the pcb board of the fan work, the pcb board and the wind Fan is electrically connected, and the temperature sensor and the pcb board are electrically connected.

10. glycosylated hemoglobin detection device as described in claim 1, which is characterized in that further include that be respectively used to will be described The fixed floor of first panel, the second panel and the support plate.