CN208206750U - Fluorescence detection device and its signal processing system, flow cytometer - Google Patents
Fluorescence detection device and its signal processing system, flow cytometer Download PDFInfo
- Publication number
- CN208206750U CN208206750U CN201820751767.1U CN201820751767U CN208206750U CN 208206750 U CN208206750 U CN 208206750U CN 201820751767 U CN201820751767 U CN 201820751767U CN 208206750 U CN208206750 U CN 208206750U
- Authority
- CN
- China
- Prior art keywords
- signal
- analog
- gain
- electrical signal
- analog electrical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
This application discloses a kind of fluorescence detection device and its signal processing systems, flow cytometer, the signal processing system of the fluorescence detection device includes the gain amplifier, analog-digital converter and controller being sequentially connected electrically, and gain amplifier is also electrically connected with the controller；Gain amplifier is for receiving analog electrical signal and feedback information, when receiving feedback information, new gain can be redefined to carry out gain process to analog electrical signal based on the input range of analog-digital converter according to feedback information, and the analog electrical signal through gain process is exported to analog-digital converter；Analog-digital converter is for receiving the analog electrical signal through gain process, being converted into digital signal and exporting to controller；Controller is handled digital signal according to predetermined manner for receiving digital signal.In this way, it is capable of increasing the range of the signal of signal processing system processing, and improves the accuracy of processing signal.
Description
Technical field
This application involves signal processing technology field, more particularly to a kind of signal processing system, fluorescence detection device and
Flow cytometer.
Background technique
Instrument comprising signal processing system, if flow cytometer is the dress that a kind of pair of cell is automatically analyzed and sorted
It sets.It can be with a series of important biophysics for the cell dispersion that rapid survey, storage, display float on a liquid, biology
The characteristic parameter of chemical aspect, and specified cell subsets can therefrom be sorted out according to the parameter ranges of pre-selection.
In use, flow cytometer is marked test object using fluorescent reagent, then to label after
Test object, which applies pressure, flows it in preset pipeline with certain speed, and uses laser irradiation, so that labeled
Test object issues fluorescence, and fluorescence signal is then converted into analog electrical signal by certain means, and further will simulation electricity
Signal is converted to digital signal, is analyzed and processed with the difference according to signal strength and obtains testing result.
However, present inventor has found in long-term R&D process, in the prior art, to analog electrical signal
When being handled, such as the fluorescence detection device of flow cytometer handles the analog electrical signal converted by fluorescence signal
When, the gain amplifier for playing amplification to the analog electrical signal can only be handled according to the gain of setting, and be difficult to reality
It now automatically adjusts, so that exceeding analog-digital converter in the range through the gain amplifier gain treated analog electrical signal
When input range, normal processing cannot be accessed, thus to limit the signal processing range of fluorescence detection device.
Utility model content
The application is mainly solving the technical problems that provide a kind of fluorescence detection device and its signal processing system, streaming are thin
Born of the same parents' instrument, is capable of increasing the range of the signal of signal processing system processing, and improves the accuracy of processing signal.
In order to solve the above technical problems, the technical solution that the application uses is: providing a kind of fluorescence detection device
Signal processing system, the signal processing system include the gain amplifier, analog-digital converter and controller being sequentially connected electrically,
Wherein, the gain amplifier is also electrically connected with the controller；The gain amplifier is for receiving analog electrical signal and anti-
Feedforward information directly carries out gain process to the analog electrical signal when not receiving the feedback information, described receiving
When feedback information, new gain is redefined according to the feedback information, gain process is carried out to the analog electrical signal, and will
Analog electrical signal through gain process is exported to the analog-digital converter；The analog-digital converter for receive it is described through gain at
The analog electrical signal after gain process is converted to digital signal by the analog electrical signal of reason, and by the digital signal
It exports to the controller；The controller is used to receive the digital signal, and according to predetermined manner to the digital signal
It is handled；Wherein, the feedback information includes the mould that the input range based on the analog-digital converter is redefined
The new gain of quasi- electric signal.
In order to solve the above technical problems, another technical solution that the application uses is: a kind of fluorescence detection device is provided,
The fluorescence detection device includes above-mentioned signal processing system.
In order to solve the above technical problems, another technical solution that the application uses is: providing a kind of flow cytometer, institute
Stating flow cytometer includes above-mentioned fluorescence detection device.
The beneficial effect of the application is: being in contrast to the prior art, the signal processing of the application fluorescence detection device
In system, gain amplifier is for receiving analog electrical signal and feedback information, when not receiving feedback information, directly to simulation
Electric signal carries out gain process, and when receiving feedback information, it is again true according to the input range institute based on analog-digital converter
The new gain of fixed analog electrical signal carries out gain process to analog electrical signal, and the analog electrical signal through gain process is defeated
Out to analog-digital converter, the analog electrical signal through gain process is converted into digital signal, and further by controller according to
Predetermined manner handles digital signal.By the above-mentioned means, the signal processing system of the application fluorescence detection device is being received
In the process of processing to the analog electrical signal being transformed by front end fluorescence signal and to it, gain amplifier can be based on
The input range of analog-digital converter gain is adjusted analog electrical signal, to reduce the input range pair of analog-digital converter
The limitation of the range of received analog electrical signal, so that the signal processing system of fluorescence detection device is capable of handling larger model
The signal enclosed, while the accuracy of processing signal can also be improved to a certain extent.
Detailed description of the invention
In order to more clearly explain the technical solutions in the embodiments of the present application, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, the drawings in the following description are only some examples of the present application, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing.Wherein:
Fig. 1 is the structural schematic diagram of one embodiment of signal processing system of the application fluorescence detection device；
Fig. 2 is the structural schematic diagram of another embodiment of signal processing system of the application fluorescence detection device；
Fig. 3 is the structural schematic diagram of the another embodiment of signal processing system of the application fluorescence detection device；
Fig. 4 is the structural schematic diagram of the another embodiment of signal processing system of the application fluorescence detection device；
Fig. 5 is the structural schematic diagram of the another embodiment of signal processing system of the application fluorescence detection device；
Fig. 6 is the structural schematic diagram of the another embodiment of signal processing system of the application fluorescence detection device；
Fig. 7 is the structural schematic diagram of one embodiment of the application fluorescence detection device；
Fig. 8 is the structural schematic diagram of one embodiment of the application flow cytometer.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present application, technical solutions in the embodiments of the present application carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of embodiments of the present application, rather than whole embodiments.Based on this
Embodiment in application, those of ordinary skill in the art are obtained every other under the premise of not making creative labor
Embodiment shall fall in the protection scope of this application.
Referring to Fig. 1, Fig. 1 is the structural representation of one embodiment of signal processing system of the application fluorescence detection device
Figure.Wherein, the signal processing system of fluorescence detection device can be applied to the multiple necks for being related to fluorescence detection in present embodiment
Domain, such as aerospace, medical treatment, household electrical appliance etc..
Specifically, in the present embodiment, by using fluorescence detection obtain include useful signal fluorescence signal, should
The signal processing system that fluorescence signal further passes through fluorescence detection device in present embodiment is handled, to be handled
As a result.
More specifically, the fluorescence detection device in present embodiment can be applied to the flow cytometer of medical field.Streaming
Cell instrument is the device that a kind of pair of cell is automatically analyzed and sorted.It can be suspended in liquid with rapid survey, storage, display
In a series of important biophysics of cell dispersion, the characteristic parameter in terms of biochemistry, and can be according to the ginseng of pre-selection
Amount range therefrom sorts out specified cell subsets.In flow cytometer, fluorescence detection device is mainly to viral, swollen
The recognition detections such as tumor, gene.Pass through the fluorescent dyes such as fluorescent dye such as phycoerythrin or phycobiliprotein or fluorescent marker
The product of polymerase chain reaction etc. is marked in the probe of specificity.Product after labeled is by certain wavelength, certain
Optical power laser excitation after, can produce the fluorescence signal of certain wavelength.Specifically, in flow cytometer, pass through control
Motor is pushed into sheath flow pool come the sample flow particle for pushing syringe that will detect, and the side of sheath flow pool is arranged laser, passes through
Focus lamp scatters out the pore of a certain size hot spot irradiation sheath flow pool glimmering after the particle stimulated light excitation of sheath flow pool the inside
Optical signal then will by certain means then further in specific angle to the fluorescence signal collection of labeled particle
Fluorescence signal is converted into analog electrical signal, and is further handled the fluorescence signal to obtain testing result.
In present embodiment, the signal processing system of fluorescence detection device includes: gain amplifier 20, analog-digital converter 40
And controller 60.Wherein, gain amplifier 20, analog-digital converter 40 and controller 60 are sequentially connected electrically, meanwhile, gain amplifier
20 are also electrically connected with controller 60.
Specifically, gain amplifier 20 is for receiving analog electrical signal and feedback information, when not receiving feedback information,
Gain process directly is carried out to analog electrical signal and redefines new gain according to feedback information when receiving feedback information
Gain process is carried out to analog electrical signal, and the analog electrical signal through gain process is exported to analog-digital converter 40.
Wherein, the 20 received analog electrical signal of institute of gain amplifier is i.e. as caused by fluorescence detection device front end or straight
The fluorescence signal received is obtained by certain conversion process.Comprising required for fluorescence detection device in the analog electrical signal
The useful information of detection needs to handle to obtain information included in it, specifically by present embodiment signal processing system
It can be current signal and/or voltage signal.Wherein, which can be the mould being directly converted to by fluorescence signal
Quasi- electric signal can also be obtained by certain pretreatment by fluorescence signal, is not especially limited herein.
Specifically, when being applied to flow cytometer, analog electrical signal refers to the fluorescence detection device in present embodiment
Pass through what conversion obtained by the fluorescence signal generated after fluorochrome label by the laser irradiation of certain wavelength by above-mentioned.It is easy
Understand ground, the signal strength of the above-mentioned analog electrical signal by fluorescence signal conversion is more faint, needs to amplify to enhance letter
Number intensity, to facilitate detection.It is further amplified by gain amplifier 20 in present embodiment, to meet detection demand,
Improve the accuracy of detection.
In present embodiment, analog electrical signal is voltage signal, and gain amplifier 20 is carrying out gain to analog electrical signal
The analog electrical signal after gain process is obtained after processing, and the first analog electrical signal is exported to analog-digital converter 40, with logical
It crosses 40 pairs of the analog-digital converter analog electrical signals after gain process and carries out analog-to-digital conversion process.
In an application scenarios, the signal processing system of the fluorescence detection device in present embodiment can further comprise
One amplitude limiter circuit, one end of the amplitude limiter circuit connect gain amplifier 20, and the other end connects analog-digital converter 40.Wherein, clipping
Circuit is the circuit that signal voltage wave amplitude can be scabbled by the range of restriction, and in present embodiment, amplitude limiter circuit specifically can be according to mould
The input range of number converter 40 limits the voltage for the first analog electrical signal that gain amplifier 20 exports, thus by mould
The limiting voltage of quasi- electric signal is within the input range of analog-digital converter 40.
Analog-digital converter 40 receives the analog electrical signal obtained by 20 gain process of gain amplifier, and is converted into
Digital signal exports the digital signal to controller 60, and controller 60 is in the number letter for receiving the output of analog-digital converter 40
After number, the digital signal is handled according to predetermined manner.
Specifically, analog-digital converter 40 can select to use high-speed AD converter according to use demand.
It is readily appreciated that ground, analog-digital converter 40 has an input range, such as 0~5V, big in the voltage of the signal of input
When 5V, it is impossible to which enough normal output, specific such as 12 analog-digital converters when the voltage of analog electrical signal is greater than 5V, pass through
The restriction of above-mentioned amplitude limiter circuit and the processing of analog-digital converter 40, will export according to maximum sampled value 4096, at this point, controller
The 60 apparently not correct numerical value received further influence the standard of testing result to will affect the processing of controller 60
True property.Meanwhile when the voltage for the analog electrical signal that analog-digital converter 40 receives is greater than its input range, illustrate that gain is amplified
Device 20 needs to reduce gain factor, so that the voltage of its analog electrical signal exported to analog-digital converter 40 meets analog-to-digital conversion
The input range of device 40.
In present embodiment, gain amplifier 20 can further receive feedback information, which includes to be based on modulus
The new gain for the analog electrical signal that the input range of converter 40 is redefined.Wherein, which can be in gain
After amplifier 20 carries out gain process to received analog electrical signal, by next amplitude limiter circuit, analog-digital converter 40
Or it when the processing such as controller 60, is detected and is issued.Such as detect this through gain amplifier 20 treated simulation electricity
When the range of signal exceeds the input range of preset analog-digital converter 40, it can be sent out according to the input range as gain amplifier 20
Feedback information is sent, specifically, in feedback information includes that gain amplifier 20 is done to required for the analog electrical signal received
New gain, so that when gain amplifier 20 carries out gain to the analog electrical signal received according to the new gain, it will
The scope control of analog electrical signal through gain process is in the input range of analog-digital converter 40, so as to by analog-to-digital conversion
Device 40 and controller 60 are normally received and are handled.
Specifically, controller 60 can be field programmable gate array FPGA controller 60, naturally it is also possible to be full
Other controllers 60 of sufficient the demand, are not specifically limited herein.
By the above-mentioned means, the signal processing system of the application fluorescence detection device is converted by front end fluorescence signal receiving
And come analog electrical signal and to it in the process of processing, gain amplifier 20 can be based on the input of analog-digital converter 40
Range gain is adjusted analog electrical signal, to reduce the input range of analog-digital converter 40 to received simulation
The limitation of the range of electric signal, so that the signal processing system of fluorescence detection device is capable of handling large range of signal, simultaneously
The accuracy of processing signal can also be improved to a certain extent.
Referring to Fig. 2, Fig. 2 is the structural representation of another embodiment of signal processing system of the application fluorescence detection device
Figure.
In present embodiment, gain amplifier 20 includes the first gain amplifier 21, and analog-digital converter 40 includes the first mould
Number converter 41.Wherein, the first gain amplifier 21, the first analog-digital converter 41 are sequentially connected electrically with controller 60, meanwhile, the
One gain amplifier 21 is also electrically connected with controller 60.
First gain amplifier 21 carries out the first gain process to analog electrical signal and obtains for receiving analog electrical signal
First analog electrical signal, and the first analog electrical signal is exported to the first analog-digital converter 41；Receive the feedback letter of controller 60
Breath, and then the second gain process is carried out to analog electrical signal and obtains the second analog electrical signal, and the second analog electrical signal is defeated
Out to the first analog-digital converter 41.
It should be pointed out that analog electrical signal received by the first gain amplifier 21 and right in present embodiment
The processing that analog electrical signal carries out etc. is identical as gain amplifier 20 in a upper embodiment, and related detailed content please refers to above-mentioned
Embodiment, details are not described herein again.
Wherein, the first gain amplifier 21 is variable gain amplifier, is specifically as follows high speed variable gain amplifier,
The first gain for carrying out the first gain process to analog electrical signal can be believed according to processing such as the first analog-digital converter 41, controllers 60
Number actual demand set, can also root such as when fluorescence detection device in the present embodiment is applied with flow cytometer
It is set according to the demand of fluidic cell project to be detected, the first different gains can be set when detecting different projects.
It, can also basis in actual use in addition, the first gain of the first gain amplifier 21 can be identical always
The progress of actual use demand is so automatic that be adaptively adjusted, and is not specifically limited herein.
In present embodiment, analog electrical signal is voltage signal, and the first gain amplifier 21 is carried out to analog electrical signal
The first analog electrical signal is obtained after first gain process, and the first analog electrical signal is exported to the first analog-digital converter 41, with
Analog-to-digital conversion process is carried out to the first analog electrical signal by the first analog-digital converter 41.
In an application scenarios, signal processing system can further comprise the first amplitude limiter circuit, first amplitude limiter circuit
One end connect the first gain amplifier 21, the other end connect the first analog-digital converter 41.
Wherein, the first amplitude limiter circuit is similar with the effect of the amplitude limiter circuit in above embodiment, can be by the first simulation
The limiting voltage of electric signal is within the input range of the first analog-digital converter 41.
First analog-digital converter 41, which is received, carries out the first mould that the first gain process obtains by the first gain amplifier 21
Quasi- electric signal, is converted to the first digital signal for the first analog electrical signal, and the first obtained digital signal is exported to control
Device 60 by controller 60 to be analyzed and processed.
Specifically, the first analog-digital converter 41 can select to use high-speed AD converter according to use demand.
Similarly, the first analog-digital converter 41 in present embodiment has an input range, received first
When the voltage range of analog electrical signal exceeds the input range, it is impossible to enough normal output, to will affect the standard of testing result
True property.
In present embodiment, the 21 received feedback information of institute of the first gain amplifier is issued by controller 60.Specifically, it controls
Device 60 processed is after the first digital signal for receiving the output of the first analog-digital converter 41, the input based on the first analog-digital converter 41
Range judges whether the first analog electrical signal exceeds the input range of the first analog-digital converter 41, is judging the first analog telecommunications
Number exceed the first analog-digital converter 41 input range when, further redefine out the first gain amplifier 21 need to simulation
Electric signal carries out the second gain process, and feeds back to the first gain amplifier 21.
Specifically, controller 60 can determine or current item detected according to the input range of the first analog-digital converter 41
Mesh come determine the first gain amplifier again to analog electrical signal carry out the second gain process the second gain.For example, operator
Member can determine the first gain amplifier 21 to analog telecommunications according to the project that the first analog-digital converter 41 and needs detect in advance
Number multiple gains, at this point, detecting that the first gain amplifier 21 needs to change gain and comes to analog electrical signal in controller 60
When carrying out the second gain process, the preparatory gain being set can be transferred further to determine.
It is readily appreciated that ground, the first gain amplifier 21 further receives the feedback information of controller 60, and obtains feedback letter
Second gain of the reset for including in breath, and second is carried out to analog electrical signal according to the second gain of the reset and is increased
Benefit reason exports second analog electrical signal to the first analog-digital converter 41 to obtain the second analog electrical signal.The
One analog-digital converter 41 receives the second analog electrical signal, and the second analog electrical signal is converted to the second digital signal, and by second
Digital signal is exported to controller 60, and controller 60 further receives the second digital signal, and divides the second digital signal
Analysis processing.
It should be pointed out that being carried out according to the feedback information of controller 60 to analog electrical signal in the first gain amplifier 21
After second gain process, obtained second analog electrical signal often meets the input range of the first analog-digital converter 41, thus
It can be the second digital signal by 41 normal conversion of the first analog-digital converter and export to controller 60 to carry out signal processing.
Certainly, in an application scenarios, by the first gain amplifier 21 according to the feedback information of controller 60 to analog electrical signal
After carrying out the second gain process, obtained second analog signal still exceeds the input range of the first analog-digital converter 41, this
When, after controller 60 receives the second digital signal can further progress detection to redefine out the first gain amplifier 21
The another gain process carried out to analog electrical signal is needed, until the analog telecommunications after 21 gain process of the first gain amplifier
It number can satisfy the input range of the first analog-digital converter 41.
It is further noted that when being applied to the fluorescence detection device of flow cytometer, in present embodiment
Signal processing system can either carry out signal processing for unitem to from which further follow that testing result, can also be directed to multiple
Project carries out signal processing simultaneously to obtain the testing result of multiple projects.
In above embodiment, signal processing system includes the first gain amplifier 21, the first modulus being sequentially connected electrically
Converter 41 and controller 60, wherein the first gain amplifier 21 is also electrically connected with controller 60.Controller 60 can to by
The first digital signal that first gain amplifier 21 carries out the first gain process and is converted to by the first analog-digital converter 41
It carries out processing and is judging to be carried out the first analog electrical signal of the first gain process by the first gain amplifier 21 beyond first
When the input range of analog-digital converter 41, the first gain amplifier 21 is redefined to the second gain process of analog electrical signal simultaneously
The first gain amplifier 21 is fed back to, to carry out the second gain process, the second analog telecommunications that will be obtained to analog electrical signal
Within the input range for number maintaining the first analog-digital converter 41, converted through the first analog-digital converter 41 in order to 60 pairs of controller
The second obtained digital signal effectively, accurately handle, and in this way, can reduce the first analog-digital converter 41
Limitation of the input range to received range of signal, so that signal processing system is capable of handling large range of signal, together
When can also further increase processing signal accuracy.
Wherein, in one embodiment, controller 60 based on the first gain amplifier 21 is carrying out analog electrical signal
After second gain process, also reduction treatment further can be carried out to the second digital signal.
It is readily appreciated that ground, judges that the first analog electrical signal exceeds the input model of the first analog-digital converter 41 in controller 60
It encloses after carrying out the second gain process to analog electrical signal to first gain amplifier 21, by obtaining for the second gain process
Second analog electrical signal is converted using the first analog-digital converter 41, at this point, if controller 60 is directly to the second obtained number
Signal is analyzed and processed, then, obtained analysis result be clearly it is not accurate enough, especially needing to carry out entry
When detection.
In present embodiment, after the first gain amplifier 21 carries out the second gain process to analog electrical signal, further
Reduction treatment is carried out to the second digital signal.For example, second gain process phase of first gain amplifier 21 to analog electrical signal
For the first gain process reduce 2 times, then, the first analog-digital converter 41 collected signal sampled value further multiplied by
2, to restore actual signal, so that signal processing system can carry out accurate and effective detection to the signal received.
In an application scenarios, after the first gain amplifier 21 carries out the second gain process to analog electrical signal, control
Device 60 processed is further marked and stores to the second digital signal being converted to through the first analog-digital converter 41, so that
When analyzing the second digital signal, effective analysis processing can be carried out to the second digital signal according to label.
Specifically for example, some second gain process of signal are reduced relative to the first gain process in finally obtained signal
2 times, some signals then reduce 4 times, at this point, controller 60 can be to the signal addition the when being analyzed and processed to reducing 2 times
One label, and the signal addition second for reducing 4 times is marked and stored, so that controller 60 in final analysis, will add
Add the signal of the first label to be further amplified 2 times, and the signal that addition second marks is further amplified 4 times, then carries out again
Analysis processing.
In addition, it is readily appreciated that ground, from the first gain amplifier 21 receive analog electrical signal carry out the first gain process to its
Receive the feedback information of controller 60 and to analog electrical signal carry out the second gain process, it is clear that have part useful signal because
The input range for having carried out the first gain process and having exceeded the first analog-digital converter 41 is unable to analyzed utilization, at this point, right
Signal by the second gain process is marked, so that controller 60 can be according further to when carrying out signal processing
Progress are more accurate to analyze for quantity of the first digital signal etc. of label, to improve the accuracy of testing result.
Wherein, referring to Fig. 3, Fig. 3 is the structural schematic diagram of another embodiment of the application signal processing system.
In present embodiment, signal processing system further include: the second gain amplifier 22 and the second analog-digital converter 42.Its
In, the second gain amplifier 22, the second analog-digital converter 42 and controller 60 are sequentially connected electrically.
In present embodiment, analog electrical signal is divided into two-way and is handled, all the way via the first gain amplifier 21 and
First analog-digital converter 41, another way is via the second gain amplifier 22 and the second analog-digital converter 42, finally by control
Device 60 handles signal.
Wherein, the second gain amplifier 22 receive analog electrical signal, and to analog electrical signal carry out third gain process and
Third analog electrical signal is obtained, and third analog electrical signal is exported to the second analog-digital converter 42.
Wherein, analog electrical signal is identical as in above embodiment, specifically refers to above embodiment, herein no longer
It repeats.Second gain amplifier 22 can be fixed gain amplifier, and the third of third gain process is carried out to analog electrical signal
Gain is fixed；In an application scenarios, the second gain amplifier 22 or variable gain amplifier are same to may be
High speed variable gain amplifier, the project that operator can detect according to the input range and needs of the second analog-digital converter 42
A fixed numbers are set to the third gain of the carry out third gain process of analog electrical signal by second gain amplifier 22,
So as to facilitate operator to debug, while can be according to different situations such as different projects to the second gain amplifier
The different gain factor of 22 settings.
Further, the second analog-digital converter 42, which is received, carries out what third gain process obtained through the second gain amplifier 22
Third analog electrical signal is converted to third digital signal, and third digital signal is exported to control by third analog electrical signal
Device 60.
In present embodiment, signal processing system can also further comprise the second amplitude limiter circuit, second amplitude limiter circuit
One end connects the second gain amplifier 22, and the other end connects the second analog-digital converter 42.
Wherein, the second amplitude limiter circuit can be identical as the amplitude limiter circuit in above embodiment, can cut by the range of restriction
Ordinary mail voltage wave amplitude, in present embodiment, the second amplitude limiter circuit specifically can be according to the input range of the second analog-digital converter 42
The voltage of the third analog electrical signal of second gain amplifier 22 output is limited, thus by the electricity of third analog electrical signal
Pressure is limited within the input range of the second analog-digital converter 42.
Wherein, the second analog-digital converter 42 can be the same or different with the first analog-digital converter 41, specifically can basis
Actual demand setting, such as when present embodiment is applied to the fluorescence detection device of flow cytometer, can detect as needed
Project and the project the different input ranges of the selection such as requirement and not the second analog-digital converter 42 of isotopic number.
In present embodiment, controller 60 further receives third digital signal, and handles third digital signal.
Wherein, the second gain amplifier 22 can be same or different with the first gain amplifier 21.In an application scenarios
In, signal processing system is when being applied to the fluorescence detection device of flow cytometer, the first gain of the first gain amplifier 21
Changeable, the third gain of the second gain amplifier 22 immobilizes, and the first gain is less than third gain.First gain amplification
Device 21 handles the biggish signal of voltage value in received analog electrical signal, can one project of fixed test or multiple signal models
Enclose lesser project；And the second gain amplifier 22 handles the lesser analog electrical signal of voltage value, thus by analog electrical signal point
It is handled simultaneously for two-way, to improve the treatment effeciency of signal processing system while increasing signal processing range.
Referring to Fig. 4, Fig. 4 is the structural schematic diagram of the another embodiment of the application signal processing system.
In present embodiment, signal processing system further includes the first low-pass filter 31 and the second low-pass filter 32,
In, 31 one end of the first low-pass filter is electrically connected the first gain amplifier 21, and the other end is electrically connected the first analog-digital converter 41, the
Two low-pass filters, 32 one end is electrically connected the second gain amplifier 22, and the other end is electrically connected the second analog-digital converter 42.
It is readily appreciated that ground, signal processing system is in the process of processing may be by the analog electrical signal received
To the interference of other signals, and it is mingled with and needs to remove into other useless signals.In present embodiment, signal processing system exists
The first low-pass filter 31 is respectively set in the two paths of setting to be filtered with the second low-pass filter 32 to signal is mingled with,
So as to improve the accuracy of signal processing system processing signal.
In addition, the connection type of the first amplitude limiter circuit and the second amplitude limiter circuit can also be the first limit in present embodiment
Width circuit on one side is electrically connected the first low-pass filter 31, and the other end is electrically connected the first analog-digital converter 41, the second amplitude limiter circuit one
End electrical connection the second low-pass filter 32, the other end be electrically connected the second analog-digital converter 42 so that the first amplitude limiter circuit and
Second amplitude limiter circuit can be respectively to by the first low-pass filter 31 and the second low-pass filter 32 treated analog signal
Carry out amplitude limiting processing.
It should be pointed out that signal processing system is applied to the fluorescence detection device of flow cytometer in the present embodiment
When, front end by the wavelength of laser that the laser of fluorescence detection device is emitted be it is known, work as in determining detection project
In, the frequency range of obtained useful signal is also known, and therefore, can select suitable first according to the frequency range
It is mingled with signal other than the frequency range to remove useful signal such as low-pass filter 31, second low-pass filter 32, to mention
The accuracy of high RST identification, to improve the accuracy of signal processing system processing signal.
Referring to Fig. 5, the structural schematic diagram of the another embodiment of Fig. 5 the application signal processing system.
In present embodiment, signal processing system further includes the early period of Signal Pretreatment subsystem 10.
It is readily appreciated that ground, the analog signal directly acquired is not it is possible that signal type meets the place of signal processing system
Situations such as reason requires, signal strength is excessively weak.In present embodiment, signal processing system further comprises Signal Pretreatment early period
System 10.Early period, Signal Pretreatment subsystem 10 was electrically connected the first gain amplifier 21 and the second gain amplifier 22,
With before the first gain amplifier 21 and the second gain amplifier 22 carry out gain process, signal early period is pre-processed and
Obtain the analog electrical signal for meeting post-processing requirement.
In addition, Signal Pretreatment subsystem is further used for the analog electrical signal that pretreatment obtains being divided into two branches,
And it is delivered to the first gain amplifier 21 and the second gain amplifier 22 respectively, further according to the application above embodiment
Analog electrical signal is handled.
Specifically, in an application scenarios, Signal Pretreatment subsystem is used for the fluorescence detection device of flow cytometer.
Referring to Fig. 6, Signal Pretreatment subsystem early period 10 includes photomultiplier tube 11, amplifier 12 and the signal being sequentially connected electrically
Conditioning circuit 13, wherein signal conditioning circuit 13 is electrically connected the first gain amplifier 21 and the second gain amplifier 22.
Wherein, photomultiplier tube 11 is used to convert the fluorescence signal of the fluorescence detection device front end received to simulation electricity
Flow signal.
However, the detection of the cell due to flow cytometry in organism, obtained fluorescence signal with
And the analog current signal that conversion obtains is very faint, the Signal Pretreatment subsystem 10 in present embodiment further comprises
Amplifier 12 suitably amplifies analog current signal.Simultaneously as the first analog-digital converter 41 and the second analog-digital converter
42 are only capable of handling voltage signal, and amplifier 12 uses preamplifier in present embodiment, further by analog current
Signal is converted into the analog voltage signal with certain voltage amplitude.
Signal conditioning circuit 13 carries out processing to analog current signal in amplifier 12 and obtains analog voltage signal laggard one
Step carries out simple RC adjusting and simple filtering etc. to the analog voltage signal, is mingled with signal suitably to weaken, thus
Enhance the output etc. of useful signal.
In aforesaid way, by by Signal Pretreatment subsystem early period 10, to acquired analog signal handled with
Obtain the analog electrical signal for meeting signal processing system post-processing demand.
It is further noted that in the prior art, in order to enable larger range of signal is handled, by front end fluorescence
Signal is divided into two-way by spectroscope to acquire, and fluorescence passes through the photomultiplier tube of low gain all the way, and fluorescence is through excessively high increasing all the way
The photomultiplier tube of benefit, and photomultiplier tube cost is relatively high, so that the cost of flow cytometer can be significantly improved, and optics
Optical path can also occupy the lightening demand that bigger structural area is unfavorable for people；In addition, in the prior art also by making
Increase the range of the signal of processing with logafier, however, losing using the signal waveform that logafier will lead to output
Very, to influence the accuracy of signal processing.And then can compared in the prior art by the signal processing system in the application
Hardware cost is enough significantly reduced, while can be avoided signal waveform distortion, improves the accuracy of signal processing.
Referring to Fig. 7, Fig. 7 is the structural schematic diagram of one embodiment of the application fluorescence detection device.
Wherein, fluorescence detection device 200 can be applied to by receive or directly generate fluorescence signal, and by fluorescence signal into
One step passes through signal processing to obtain the instrument of testing result, for example, can be used for the flow cytometer etc. of medical field.
In present embodiment, fluorescence detection device 200 includes such as signal processing system 100, to connect to fluorescence signal
It receives and processing is analyzed.Specifically, the signal in signal processing system 100 and above-mentioned the application signal processing system embodiment
Processing system is identical, and related detailed content please refers to above embodiment, and details are not described herein again.
Referring to Fig. 8, Fig. 8 is the structural schematic diagram of one embodiment of the application flow cytometer.In present embodiment, stream
Formula cell instrument 300 includes fluorescence detection device 200.Specifically, fluorescence detection device 200 and above-mentioned the application fluorescence detection device
Identical in embodiment, related detailed content please refers to above embodiment, and details are not described herein again.
It should be pointed out that controller is identified and is analyzed to front end fluorescence signal, is obtained in flow cytometer
Testing result, and being carried out data transmission by data/address bus and processor, is finally uploaded to computer, by the display of computer with
The index parameters such as interface scatter plot and parameter are shown.Meanwhile in debugging process early period, operator is controlled by computer
And the gain of the first gain amplifier and the first gain amplifier is adjusted, to facilitate the debugging of operator.
The foregoing is merely presently filed embodiments, are not intended to limit the scope of the patents of the application, all to utilize this
Equivalent structure or equivalent flow shift made by application specification and accompanying drawing content, it is relevant to be applied directly or indirectly in other
Technical field similarly includes in the scope of patent protection of the application.
Claims (12)
1. a kind of signal processing system of fluorescence detection device, which is characterized in that the signal processing system includes: successively to be electrically connected
Gain amplifier, analog-digital converter and the controller connect, wherein the gain amplifier is also electrically connected with the controller；
The gain amplifier is for receiving analog electrical signal and feedback information, when not receiving the feedback information, directly
Gain process is carried out to the analog electrical signal to be redefined when receiving the feedback information according to the feedback information
New gain carries out gain process to the analog electrical signal, and the analog electrical signal through gain process is exported to the modulus
Converter；
The analog-digital converter is for receiving the analog electrical signal through gain process, by the simulation after gain process
Electric signal is converted to digital signal, and the digital signal is exported to the controller；
The controller is handled the digital signal according to predetermined manner for receiving the digital signal；
Wherein, the feedback information includes the analog telecommunications that the input range based on the analog-digital converter is redefined
Number new gain.
2. signal processing system according to claim 1, which is characterized in that the gain amplifier includes that the first gain is put
Big device, the analog-digital converter include the first analog-digital converter, wherein first gain amplifier, first modulus turn
Parallel operation and the controller are sequentially connected electrically, and first gain amplifier is also electrically connected with the controller；
First gain amplifier carries out the first gain process for receiving the analog electrical signal, to the analog electrical signal
And the first analog electrical signal is obtained, and first analog electrical signal is exported to first analog-digital converter；Described in reception
The feedback information of controller, and then the second gain process is carried out to the analog electrical signal and obtains the second analog telecommunications
Number, and second analog electrical signal is exported to first analog-digital converter；
First analog-digital converter is converted to for receiving first analog electrical signal, by first analog electrical signal
One digital signal, and first digital signal is exported to the controller；Second analog electrical signal is received, it will be described
Second analog electrical signal is converted to the second digital signal, and second digital signal is exported to the controller；
The controller for receiving first digital signal, judge by the input range based on first analog-digital converter
First analog electrical signal exceeds the input range of first analog-digital converter, and then redefines out first gain
Amplifier needs to carry out the second gain process to the analog electrical signal, and feeds back to first gain amplifier；Receive institute
The second digital signal is stated, and second digital signal is analyzed and processed.
3. signal processing system according to claim 2, which is characterized in that the gain amplifier further includes the second gain
Amplifier, the analog-digital converter further include the second analog-digital converter, wherein second gain amplifier, second mould
Number converter and the controller are sequentially connected electrically；
Second gain amplifier carries out third gain process for receiving the analog electrical signal, to the analog electrical signal
And third analog electrical signal is obtained, and the third analog electrical signal is exported to second analog-digital converter；
Second analog-digital converter is converted to for receiving the third analog electrical signal, by the third analog electrical signal
Three digital signals, and the third digital signal is exported to the controller；
The controller is also used to receive the third digital signal, and is analyzed and processed to the third digital signal.
4. signal processing system according to claim 3, which is characterized in that the system also includes the first low-pass filters
With the second low-pass filter, wherein first low-pass filter one end is electrically connected first gain amplifier, other end electricity
First analog-digital converter is connected, second low-pass filter one end is electrically connected second gain amplifier, the other end
It is electrically connected second analog-digital converter.
5. signal processing system according to claim 3, which is characterized in that the system also includes Signal Pretreatments early period
Subsystem, the Signal Pretreatment early period subsystem are electrically connected first gain amplifier and second gain amplification
Device, the Signal Pretreatment subsystem is for pre-processing signal early period and obtaining analog electrical signal, by the simulation electricity
Signal is divided into two branches, and is delivered to first gain amplifier and second gain amplifier respectively.
6. signal processing system according to claim 5, which is characterized in that the Signal Pretreatment early period subsystem includes
Photomultiplier tube, amplifier and the signal conditioning circuit being sequentially connected electrically, wherein the signal conditioning circuit is electrically connected
First gain amplifier and second gain amplifier.
7. signal processing system according to claim 3, which is characterized in that the first gain of first gain amplifier
Changeable, the third gain of second gain amplifier immobilizes, and first gain is less than second gain.
8. signal processing system according to claim 1, which is characterized in that the controller is field programmable gate
Array FPGA controller.
9. signal processing system according to claim 2, which is characterized in that the controller is also used to increase described first
After the second gain process that beneficial amplifier carries out the analog electrical signal, reduction treatment is carried out to second digital signal.
10. signal processing system according to claim 9, which is characterized in that the controller is also used to described second
Digital signal is marked and stores, and the label can be different from first digital signal, and makes the controller can be with
Reduction treatment is carried out to second digital signal according to the label.
11. a kind of fluorescence detection device, which is characterized in that the fluorescence detection device includes such as any one of claim 1-10 institute
The signal processing system stated.
12. a kind of flow cytometer, which is characterized in that the flow cytometer includes fluorescence detection as claimed in claim 11
Device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820751767.1U CN208206750U (en) | 2018-05-18 | 2018-05-18 | Fluorescence detection device and its signal processing system, flow cytometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820751767.1U CN208206750U (en) | 2018-05-18 | 2018-05-18 | Fluorescence detection device and its signal processing system, flow cytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208206750U true CN208206750U (en) | 2018-12-07 |
Family
ID=64518743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820751767.1U Active CN208206750U (en) | 2018-05-18 | 2018-05-18 | Fluorescence detection device and its signal processing system, flow cytometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208206750U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110501277A (en) * | 2018-05-18 | 2019-11-26 | 深圳市帝迈生物技术有限公司 | Fluorescence detection device and its signal processing system, method, flow cytometer |
-
2018
- 2018-05-18 CN CN201820751767.1U patent/CN208206750U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110501277A (en) * | 2018-05-18 | 2019-11-26 | 深圳市帝迈生物技术有限公司 | Fluorescence detection device and its signal processing system, method, flow cytometer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204613066U (en) | A kind of dust detecting sensor based on LASER Light Source | |
| CN103529006B (en) | A kind of Portable fluorescence based on micro-fluidic chip detection device | |
| CN101403739B (en) | Cell analyzer and cell analyzing method | |
| CN110501277A (en) | Fluorescence detection device and its signal processing system, method, flow cytometer | |
| JP4911601B2 (en) | Method for measuring nucleated red blood cells | |
| JP6232046B2 (en) | Urine sample analyzer and urine sample analysis method | |
| JP6116502B2 (en) | Sample analyzer and sample analysis method | |
| CN108287129A (en) | The detection device of multichannel fluorescence Spectra bioaerosol particle | |
| WO2011159708A1 (en) | System and method for creating a flow cytometer network | |
| CN102147365A (en) | Handheld bioluminescent detector and detection method | |
| CN103822868A (en) | Device and method for detecting grain size of phytoplankton in seawater | |
| CN107462560A (en) | LIF combinations LIBS edible oil quality fast analyser and method | |
| CN108279229B (en) | Whole blood CRP detection device | |
| CN106019350A (en) | Nuclear pulse signal acquisition device and system | |
| CN208206750U (en) | Fluorescence detection device and its signal processing system, flow cytometer | |
| CN204128960U (en) | A kind of drinks quality sense organ classification quick analytic instrument | |
| CN110887818A (en) | Blood sample analysis method, blood cell analyzer and storage medium | |
| CN102967557B (en) | Biological aerosol water-content determination based on near infrared spectroscopy and classification method thereof | |
| CN107314994B (en) | Fluorescent signal gain adjusting method and device | |
| EP3510137A1 (en) | Automated body fluid analysis | |
| CN208443697U (en) | A kind of nano particle diameter distribution detector | |
| CN204575538U (en) | A kind of laser-induced fluorescence (LIF) food detector | |
| CN201229205Y (en) | Portable plant flower color assorting and growth status detection device | |
| CN104458541A (en) | Analysis method for red blood cell hemoglobin content, device and blood cell analyzer | |
| CN204882371U (en) | Digital photoelectricity biosensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PP01 | Preservation of patent right |
Effective date of registration: 20200528 Granted publication date: 20181207 |
|
| PP01 | Preservation of patent right | ||
| PD01 | Discharge of preservation of patent |
Date of cancellation: 20220919 Granted publication date: 20181207 |
|
| PD01 | Discharge of preservation of patent |