CN208140587U - A kind of fiber Raman detection bacterium device based on SERS substrate - Google Patents

Abstract

The utility model discloses a kind of fiber Raman detection bacterium device based on SERS substrate, including import optical fiber, export optical fiber, No.1 convex lens, No. two convex lenses, optical filter, SERS substrate, through-hole, transmitter, monochromator, detector；Laser emitting module and light collect detection module and carry out light signal transduction by U-shaped optical fiber.SERS substrate is loaded on U-shaped optical fiber as solid phase carrier, with antibody coupling, water body to be detected is immersed, the bacterium in water body is made to generate specific immune response as antigen and antibody, detection front and back carries out Raman spectrum comprehensive analysis, can detect to qualitative, quantitative water-outlet body bacterium.The utility model simplifies Bacteria Detection process, without being pre-processed to water body, reduce operation bring pollution, improve the reliability of testing result, device uses U-shaped optical fiber and is loaded with the up-side down triangle through-hole structure of SERS substrate simultaneously, reduces incident light and scatters the interference of light, enhancing detection signal, improve detection accuracy, and reusable.

Description

A kind of fiber Raman detection bacterium device based on SERS substrate

Technical field

The utility model relates to water body Bacteria Detection field more particularly to a kind of fiber Raman detections based on SERS substrate Bacterium device.

Background technique

Germ contamination is the importance of water pollution, for the safety for preferably ensureing drinking water and domestic water, is needed Quick, accurate, efficiently, the inexpensive water body bacteria detecting apparatus of development.Common method currently used for detecting water body bacterium has Filter membrane method, MTF method, round pcr etc., but the generally existing sensitivity of these methods is not high and detection process is cumbersome etc. lacks Point.To overcome the above disadvantages, Raman technology by its high sensitivity, detection speed it is fast, fluorescence background is low, to test sample without The advantages that damage, realizes in situ, the quick detection of water body bacterium.But to optic path process when being detected with the technology Middle signal strength requirement is high, and fiber optic materials have decay small, bandwidth, strong interference immunity etc. excellent when carrying out light conduction Point, therefore be widely used as the material of Raman light signal transduction.If number of patent application is 201620839394.4, Patent name is the utility model patent of Portable Raman spectrometer, realizes quick inspection of the optical fiber transmission Raman light to sample It surveys, but its device can not achieve the direct detection to measured object, and sample is easily caused to pollute, and influence the accuracy of detection；Patent Application No. is 201510023219.8, patent name is a kind of hair of method in conical fiber surface-assembled noble metal nanocrystalline Bright patent, have stronger SERS sensibility, signal stabilization, but due to its incident light and scattering light locate in same optical path, meeting It interferes, is unfavorable for optical signal detecting, and the optical fiber detector cannot be reused.

Summary of the invention

For the above-mentioned problems in the prior art, the purpose of this utility model is to provide one kind to be based on SERS substrate Fiber Raman detection bacterium device, its object is to can effectively to bacterium carry out quick specific immunity capture and Noiseless transmission is carried out to optical signal, improves detection accuracy, and reusable.

In order to solve the above problem, the utility model adopts the following technical solution：

A kind of fiber Raman detection bacterium device based on SERS substrate, including laser emitting module（1）, U-shaped optical fiber passes Guide module（2）Detection module is collected with optical signal（3）；Wherein laser emitting module（1）Including transmitter（101）, optical filter （102）, No.1 convex lens（103）；The optical filter（102）Positioned at transmitter（101）Left side, and face transmitter （101）Laser port, optical filter（102）With No.1 convex lens（103）Optical axis is conllinear, and No.1 convex lens（103）Positioned at filter Mating plate（102）Left side；U-shaped fiber optic conduction module（2）Including importing optical fiber（201）, export optical fiber（204）, through-hole（202）, SERS substrate（203）；The importing optical fiber（201）With export optical fiber（204）For same root multimode fiber, diameter is 400 ~ 1000μm；The through-hole（202）Be by femtosecond laser diaphragm be 5, energy 200mW, pulse frequency be 1kHz condition Under the equilateral triangle through-hole that is process, side length is 100 μm, is located at U-shaped fiber optic conduction module（2）Fibre-optical bending at just It is intermediate；The SERS substrate（203）It is silicon wafer, through-hole is fixed on by inverted triangular structure feature（202）Triangle bottom Bian Shang, and it is detachable, and nano-Ag particles are adhered to using electrostatic absorption principle in surface；Optical signal collects detection module（3）Packet Include detector（301）, monochromator（302）, No. two convex lenses（303）；The monochromator（302）Positioned at detector（301）It is left Side, and monochromator（302）Exit slit face detector（301）Light collection port, monochromator（302）Entrance slit and No. two convex lenses（303）Optical axis is conllinear, and No. two convex lenses（303）Positioned at monochromator（302）Left side；The Laser emission Module and importing optical fiber（201）It is connected, optical signal collects detection module and export optical fiber（204）It is connected.

Above-mentioned technical proposal has the advantages that：

1. using U-shaped optical fiber structure, reducing incident light and scattering the interference of light, detection accuracy is improved；

2. can directly be detected to water body bacterium using the device, without pre-processing to water body, operation is reduced Bring pollution, improves the reliability of testing result；

3. device has used up-side down triangle through-hole, convenient for fixed SERS substrate, and reduce light in transmission process Loss；

4. using SERS substrate, detection signal strength is enhanced, and fix using inverted triangular structure feature, conveniently torn open It unloads, the different substrate of energy repeated detection enables the device to reuse.

Detailed description of the invention

With reference to the accompanying drawing and embodiment is described further the utility model：

Fig. 1 is the utility model structure chart；

Fig. 2 is the partial enlarged view of U-shaped optical fiber；

In figure 1 be laser emitting module, 2 be U-shaped fiber optic conduction module, 3 be optical signal collect detection module, 101 be hair Emitter, 102 be optical filter, 103 be No.1 convex lens, 201 be import optical fiber, 202 be through-hole, 203 be SERS substrate, 204 be Export optical fiber, 301 be detector, 302 be monochromator, 303 be No. two convex lenses.

Specific embodiment

In Fig. 1, Fig. 2, a kind of fiber Raman detection bacterium device based on SERS substrate, including laser emitting module 1, U Type fiber optic conduction module 2 and optical signal collect detection module 3；Wherein laser emitting module 1 includes transmitter 101, optical filter 102, No.1 convex lens 103；The optical filter 102 is located at 101 left side of transmitter, and the laser port of face transmitter 101, Optical filter 102 is conllinear with 103 optical axis of No.1 convex lens, and No.1 convex lens 103 is located at 102 left side of optical filter；U-shaped optical fiber passes Guide module 2 includes importing optical fiber 201, export optical fiber 204, through-hole 202, SERS substrate 203；It the importing optical fiber 201 and leads Optical fiber 204 is same root multimode fiber out, and diameter is 400 ~ 1000 μm；The through-hole 202 is 5 in diaphragm by femtosecond laser, Energy is 200mW, and the equilateral triangle through-hole that pulse frequency is process under conditions of being 1kHz, side length is 100 μm, is located at U Middle at the fibre-optical bending of type fiber optic conduction module 2；The SERS substrate 203 is silicon wafer, passes through inverted triangular structure Feature is fixed on the triangle base of through-hole 202, and detachable, and nano silver is adhered to using electrostatic absorption principle in surface Grain；It includes 302, No. two detector 301, monochromator convex lenses 303 that optical signal, which collects detection module 3,；The monochromator 302 In 301 left side of detector, and the light collection port of the exit slit face detector 301 of monochromator 302, monochromator 302 enter It penetrates slit and No. two 303 optical axises of convex lens is conllinear, and No. two convex lenses 303 are located at 302 left side of monochromator；The laser hair It penetrates module 1 and is connected with optical fiber 201 is imported, optical signal collects detection module 3 and is connected with export optical fiber 204.

A kind of fiber Raman detection bacterium device working principle based on SERS substrate：Fiber Raman based on SERS substrate Detection bacterium device is connected according to structure shown in FIG. 1, and SERS substrate 203 is fixed on through-hole 202, and U-shaped optical fiber immersion contains There is the solution of specific antibodies, the SERS substrate 203 in knee is made to be used as solid phase carrier and antibody coupling, starts laser 101, hair The laser of 785nm out makes laser pass sequentially through optical filter 102, No.1 convex lens 103, is passed to U-shaped optical fiber from optical fiber 201 is imported Module 2 is conducted, the light scattered on SERS substrate 203 assembles back into optical fibers by the triangular facet reflection of through-hole 202, from export optical fiber 204 outflows, then No. two convex lenses 303, monochromator 302, detector 301 are passed sequentially through, optical signal is collected by detector 301, it After will test device 301 and be connected with signal analyzer, obtain first group of Raman spectrogram, after immerse water body to be detected again, make water Bacterium in body generates specific immune response as antigen and antibody, captures bacterium, is again started up laser 101, issues The laser of 785nm makes laser pass sequentially through optical filter 102, No.1 convex lens 103, is passed to U-shaped optical fiber biography from optical fiber 201 is imported Guide module 2, the light scattered on SERS substrate 203 assemble back into optical fibers by the triangular facet reflection of through-hole 202, from export optical fiber 204 outflows, then No. two convex lenses 303, monochromator 302, detector 301 are passed sequentially through, optical signal is collected by detector 301, it After will test device 301 and be connected with signal analyzer, obtain second group of Raman spectrogram, the two groups of Raman spectrograms compared Characteristic peak and carry out algorithm analysis, can it is qualitative, quantitatively detect bacterium in water-outlet body.

Claims (3)

1. a kind of fiber Raman detection bacterium device based on SERS substrate, including laser emitting module（1）, U-shaped fiber optic conduction Module（2）Detection module is collected with optical signal（3）；Wherein laser emitting module（1）Including transmitter（101）, optical filter （102）, No.1 convex lens（103）；The optical filter（102）Positioned at transmitter（101）Left side, and face transmitter （101）Laser port, optical filter（102）With No.1 convex lens（103）Optical axis is conllinear, and No.1 convex lens（103）Positioned at filter Mating plate（102）Left side；Optical signal collects detection module（3）Including detector（301）, monochromator（302）, No. two convex lenses （303）；The monochromator（302）Positioned at detector（301）Left side, and monochromator（302）Exit slit face detection Device（301）Light collection port, monochromator（302）Entrance slit and No. two convex lenses（303）Optical axis is conllinear, and No. two convex lens Mirror（303）Positioned at monochromator（302）Left side；It is characterized in that, U-shaped fiber optic conduction module（2）Including importing optical fiber（201）, lead Optical fiber out（204）, through-hole（202）, SERS substrate（203）；The through-hole（202）It is equilateral triangle through-hole, is located at U-shaped light Fibre conduction module（2）Fibre-optical bending at middle；The SERS substrate（203）It is silicon wafer, is fixed on through-hole（202）'s On triangle base, nano-Ag particles are adhered on surface；The importing optical fiber（201）With laser emitting module（1）It is connected, leads Optical fiber out（204）Detection module is collected with optical signal（3）It is connected.

2. a kind of fiber Raman detection bacterium device based on SERS substrate according to claim 1, which is characterized in that institute The U-shaped fiber optic conduction module stated（2）For same root multimode fiber, diameter is 400 ~ 1000 μm.

3. a kind of fiber Raman detection bacterium device based on SERS substrate according to claim 1, which is characterized in that institute The equilateral triangle through-hole side length stated is 100 μm.