CN208131057U - fluid driving mechanism - Google Patents

fluid driving mechanism Download PDF

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Publication number
CN208131057U
CN208131057U CN201820121240.0U CN201820121240U CN208131057U CN 208131057 U CN208131057 U CN 208131057U CN 201820121240 U CN201820121240 U CN 201820121240U CN 208131057 U CN208131057 U CN 208131057U
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liquid
pipette tips
outlet end
spitting
liquid pipette
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CN201820121240.0U
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盛广济
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Sinafo (Suzhou) Life Technology Co.,Ltd.
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Beijing Optical Trap Management Consulting Partnership (limited Partnership)
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Abstract

The utility model relates to a kind of fluid driving mechanism, generate system, including capacity component and Power Component for microlayer model.Capacity component includes injection tube and push rod, the inner wall of push rod and injection tube is slidably matched, and driving liquid can be stored in injection tube, and injection tube has liquid in-out mouth, liquid in-out mouth is sequentially connected for being connected to the arrival end for spitting liquid pipette tips for storing the first liquid, Power Component and push rod.In the generating process of microlayer model, the extruding of Power Component driving push rod, which is stored in injection tube, drives liquid, and driving liquid extruding is stored in the first liquid spat in liquid pipette tips, and then the first liquid is discharged from the outlet end for spitting liquid pipette tips.Fluid driving mechanism provided by the utility model ensure that the outlet end for spitting liquid pipette tips remains to that the first liquid is discharged from the outlet end for spitting liquid pipette tips according to the flow velocity of setting when high-frequency is vibrated using the Incoercibility of driving liquid.

Description

Fluid driving mechanism
Technical field
The utility model relates to the measurements of micro liquid, distribution technique field, more particularly to a kind of fluid driving mechanism.
Background technique
It examines at present in clinical medicine, nano material preparation, the application fields such as food and environment measuring, biochemical analysis have To the widespread demand of micro liquid precise manipulation.The core technology of submicroliter fluid handling first is that the liquid of microlitre magnitude into One step is divided into nanoliter micro- reaction system of even picoliters volume.The major technique branch that micro- reaction system generates is emulsification Microlayer model generates.
In recent years, a variety of microlayer model generation techniques are reported in the literature, such as membrane emulsification, spraying emulsion process, miniflow Control chip method, spit liquid pipette tips injection/gunite etc..Wherein, liquid pipette tips injection/gunite, which is spat, as newest microlayer model generates skill Art all has good application prospect in terms of the generation of microlayer model and in terms of consumables cost control.Generate the process of microlayer model In, the outlet end for spitting liquid pipette tips is kept in motion, and the flow velocity that liquid is discharged is unstable, uncontrollable.Microlayer model body generated Randomness is presented in product size.
Utility model content
Based on this, it is necessary to for spit liquid pipette tips during exercise because discharge liquid flow velocity it is unstable, it is uncontrollable caused by The problem of microlayer model volume size shows randomness, providing one kind can guarantee to spit liquid pipette tips according to setting flow velocity discharge liquid Fluid driving mechanism.
A kind of fluid driving mechanism generates system for microlayer model, including：
Capacity component, including injection tube and push rod, the inner wall of the push rod and the injection tube are slidably matched, the note Driving liquid can be stored in shooting cylinder, the injection tube has liquid in-out mouth, and the liquid in-out mouth stores first for being connected to The arrival end for spitting liquid pipette tips of liquid；
Power Component is sequentially connected with the push rod, for driving the push rod sliding along the extending direction of the injection tube It is dynamic；
In the generating process of microlayer model, the Power Component drives the push rod extruding to be stored in the injection tube Liquid is driven, driving liquid extruding is stored in the first liquid spat in liquid pipette tips, and then by the first liquid from spitting going out for liquid pipette tips The discharge of mouth end.
A kind of fluid driving method, using the above scheme described in any item fluid driving mechanism, the fluid driving side Method includes：The Power Component drives the push rod to squeeze the driving liquid being stored in the injection tube, the driving Liquid, which squeezes, is stored in first liquid spat in liquid pipette tips, and first liquid is from the outlet end for spitting liquid pipette tips Discharge.
Above-mentioned fluid driving mechanism ensure that the outlet end for spitting liquid pipette tips in high frequency using the Incoercibility of driving liquid Rate remains to that the first liquid is discharged from the outlet end for spitting liquid pipette tips according to the flow velocity of setting when vibrating.Stream provided by the utility model Body driving mechanism can accurately control generated microlayer model volume size.
Detailed description of the invention
Fig. 1 is the overall structure diagram of digital pcr detector provided by the invention；
Fig. 2 is the microlayer model generating means of digital pcr detector provided by the invention；
Fig. 3 is the stress diagram of drop when exporting end motion for spitting liquid pipette tips that one embodiment of the invention provides；
Fig. 4 is the velocity variations schematic diagram for the outlet end for spitting liquid pipette tips that one embodiment of the invention provides；
Microlayer model generating process schematic diagram when the outlet end motion for spitting liquid pipette tips that Fig. 5 provides for one embodiment of the invention；
Fig. 6 is the outlet end structure schematic diagram for spitting liquid pipette tips that one embodiment of the invention provides；
Fig. 7 be another embodiment of the present invention provides the outlet end structure schematic diagram for spitting liquid pipette tips；
Fig. 8 spits liquid pipette tips structural schematic diagram for what one embodiment of the invention provided；
Fig. 9 be another embodiment of the present invention provides spit liquid pipette tips structural schematic diagram；
Figure 10 is the process schematic that the chamfered structure that one embodiment of the invention provides spits that liquid pipette tips generate microlayer model；
Figure 11 be another embodiment of the present invention provides chamfered structure spit liquid pipette tips generate microlayer model process schematic；
Figure 12 is the process schematic that the bending structure that one embodiment of the invention provides spits that liquid pipette tips generate microlayer model；
Figure 13 be another embodiment of the present invention provides bending structure spit liquid pipette tips generate microlayer model process schematic；
Figure 14 is the flow control mechanism and spit liquid pipette tips connection schematic diagram that one embodiment of the invention provides；
Figure 15 is the flow control mechanism structural schematic diagram that one embodiment of the invention provides；
Figure 16 is that the liquid pipette tips of spitting that one embodiment of the invention provides generate the signal of microlayer model process in the case where driving liquid driven Figure；
Figure 17 be another embodiment of the present invention provides flow control mechanism structural schematic diagram.
Wherein：
1- digital pcr detector；10- microlayer model generating means；20- temperature control device；30- fluorescence signal detection device；40- Quantitative analysis device；50- controller；110- spits liquid pipette tips；111- arrival end；The outlet end 112-；113- needle stalk；114- pintle； 115- reservoir；116- card slot；The first liquid of 190-；195- drop；199- microlayer model；120- fluid driving mechanism；121- becomes Volume component；1211- injection tube；1212- push rod；1213- liquid in-out mouth；1214- drives liquid；122- Power Component；1221- Driving motor；1222- screw rod；1223- sliding block；123- tubule；124- three-way diverter valve；125- fluid reservoir；130- motion control Mechanism；The first controller of 170-；60- microlayer model container；699- second liquid；F1- buoyancy；F2- viscosity resistance；F3- maximum is attached Put forth effort；G- gravity.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, by the following examples, it and combines attached Figure, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only to explain this hair It is bright, it is not intended to limit the present invention.
It should be noted that it can directly on the other element when element is referred to as " being fixed on " another element Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to To another element or it may be simultaneously present centering elements.On the contrary, when element is referred to as " directly existing " another element "upper", There is no intermediary elements.Term as used herein "vertical", "horizontal", "left" and "right" and similar statement are For illustrative purposes.Various difference objects are in the ratio drafting convenient for enumerating explanation in embodiment attached drawing, rather than press practical group The ratio of part is drawn.
Digital pcr (Digital PCR, dPCR) is a kind of nucleic acid molecules absolute quantitation technology.Compared to qPCR, number PCR can allow you that can directly count the number of DNA molecular, be the absolute quantitation to initial sample.Quantitative PCR is bent by standard Line or reference gene measure nucleic acid amount, and digital pcr then allows you that can directly count the number of DNA molecular, be to starting sample The absolute quantitation of product.
Currently, digital pcr includes drop formula PCR detection method and chip type detection method.It is single in chip type detection method Effecting reaction chamber quantity on a chip is generally only thousands of, far fewer than drop formula.So the dynamic of chip type digital pcr Range is relatively narrow relative to drop formula.Sample is dispersed into the reaction member of Water-In-Oil by drop formula PCR detection method, later to each Reaction member carries out in real time or end point fluorescence is analyzed.But current digital pcr instrument is there are effecting reaction element number is few, Consumables cost is high, dynamic range is relatively narrow, the problem that working efficiency is low and integration degree is not high.
Based on this, it is necessary to aiming at the problem that current digital pcr instrument, provide a kind of digital pcr detector.
Referring to Figure 1, the present invention provides a kind of digital pcr detector 1, and the digital pcr detector 1 includes：Microlayer model Generating means 10, temperature control device 20, fluorescence signal detection device 30, quantitative analysis device 40 and controller 50.Micro- liquid Generating means 10 are dripped to form multiple microlayer models for nucleic acid amplification reaction liquid droplet.The temperature control device 20 with it is described micro- Drop formation device 10 is connected by track, the multiple microlayer model is transferred to the temperature control device 20, carries out temperature Circulation realizes nucleic acid amplification.The fluorescence signal detection device 30 is oppositely arranged with the temperature control device 20, to expand nucleic acid The multiple microlayer model after increasing carries out detection of taking pictures.The quantitative analysis device 40 and the fluorescence signal detection device 30 are logical Data line connection is crossed, to realize the transmission of the multiple microlayer model fluorescence information, carries out quantitative analysis.The controller 50 divides Not with the microlayer model generating means 10, the temperature control device 20, fluorescence signal detection device 30 and quantitative analysis device 40 Connection, to control the microlayer model generating means 10, the temperature control device 20, fluorescence signal detection device 30 and quantitative point Analysis apparatus 40.
The digital pcr detector 1 can be by the microlayer model generating means 10, the temperature control device 20, the fluorescence Signal supervisory instrument 30 and the quantitative analysis device 40 are integrated, so that automation behaviour may be implemented in operator Make.The digital pcr detector 1 working efficiency with higher.
At work, the microlayer model generating means 10 can expand the determined nucleic acid digital pcr detector 1 Reaction solution carries out droplet, to form multiple microlayer models.The temperature control device 20 can carry out core to the multiple microlayer model Acid amplification.The fluorescence signal detection device 30 claps the change in fluorescence picture for surveying the multiple microlayer model in real time.By described more The change in fluorescence picture of a microlayer model, the change in fluorescence curve of available the multiple microlayer model.According to the change in fluorescence Curve, the Ct value of available the multiple microlayer model, and by the relationship of Ct value and starting copy number to the concentration of initial DNA Carry out quantitative analysis.Wherein, Ct value refers to recurring number experienced when the fluorescence signal of each microlayer model reaches the threshold value of setting.
The temperature control device 20 carries out nucleic acid amplification reaction to the multiple microlayer model, and is detected by the fluorescence signal Device 30 acquires the product signal of the multiple microlayer model after nucleic acid amplification reaction, such as fluorescence, UV absorption, turbidity letter Number.Difference using the multiple amplification with non-amplification microlayer model in composition, to the amount of droplets for obtaining target sequence amplification It is analyzed, the final quantitative analysis realized to nucleic acid molecules.Pass through the change in fluorescence figure of the multiple microlayer model of real-time monitoring The problem of piece, testing result has substantivity, can solve the false positive and false negative in the multiple microlayer model.
The digital pcr detector 1 is by the microlayer model generating means 10, the temperature control device 20, the fluorescence signal Detection device 30 and the quantitative analysis device 40 are integrated, so that automatic operation may be implemented in the operator, no Into improving work efficiency, also have the advantages that rapid reaction, reproducible, high sensitivity, high specificity and result are clear.
Clinical medicine is examined at present, nano material preparation, the application fields such as food and environment measuring, biochemical analysis are all to micro- Quantity of fluid precise manipulation has widespread demand.The core technology of submicroliter fluid handling first is that the liquid microlitre magnitude is further It is divided into nanoliter the drop of even picoliters volume, as micro- reaction system.The major technique branch that micro- reaction system generates It is that emulsification microlayer model generates.
In recent years, a variety of microlayer model generation techniques are reported in the literature, such as membrane emulsification, spraying emulsion process, miniflow It controls chip method and spits liquid pipette tips injection/gunite.However, generating the method for emulsification microlayer model in practical application by spitting liquid pipette tips In all respectively have some disadvantages.Interface energy and fluid shearing of some methods using micro liquid when gas liquid film converts Power overcomes liquid in the surface tension and adhesive force of spitting the outlet of liquid pipette tips, outflow is enable to spit the drop of liquid pipette tips nozzle successfully Liquid pipette tips are spat in disengaging, and the controllable drop of size is formed in immiscible liquid.But this method needs to spit liquid pipette tips in liquid Cutting movement above and below face, it is also necessary to high-precision positioning is carried out to starting and final position of the liquid pipette tips relative to liquid level is spat, It is difficult in Project Realization.The above method is during spitting liquid pipette tips quickly disengaging liquid phase repeatedly, the easy shape in the surface of liquid phase At unstable standing wave, the generating rate of microlayer model is limited.Method also by spit circumference of the liquid pipette tips in liquid or The shearing force that person's spiral uniform motion generates cuts off the immiscible liquid of injection and forms drop.But this method is due to spitting The size that liquid pipette tips generate drop is affected by various system factor variations (such as viscosity, the temperature of environment of liquid Degree, movement velocity, motion profile etc.), to generate error.Also, this error can with generate drop quantity increase and Accumulation, thus the control of the volume size homogeneity of high-volume drop formation is difficult.
Based on this, it is necessary to for existing microlayer model generating rate is relatively slow during generating microlayer model, it is micro- to generate The uncontrollable problem of drop volume size homogeneity, provides that a kind of microlayer model quickly generates and that volume size homogeneity is high is micro- Drop formation method and device.
Fig. 2 is referred to, in one embodiment, the microlayer model generating means 10 include spitting liquid pipette tips 110, fluid driving Mechanism 120, motion control mechanism 130 and the first controller 170.The liquid pipette tips 110 of spitting have outlet end and arrival end, and For storing the first liquid.Microlayer model generating means 10 can be used cooperatively with microlayer model container.It is stored up in the microlayer model container There is second liquid, the outlet end for spitting liquid pipette tips 110 is inserted under the liquid level of the second liquid.
Between first liquid and the second liquid it is immiscible or have interfacial reaction.First liquid and the second liquid Body can be any immiscible two kinds of liquid, and in one embodiment of the invention, first liquid is aqueous solution, described Second liquid be and the immiscible oil-based liquid of water, such as mineral oil (including n-tetradecane), vegetable oil, silicone oil and perfluoro alkane Oil etc., the drop of generation are aqueous solution droplets.Alternatively, first liquid be mineral oil, such as the tetradecane and n-hexane it is organic Phase, the second liquid are and the immiscible perfluorine oil of mineral oil.First liquid and second liquid can be not mutual Molten aqueous two-phase, in another embodiment of the present invention, first liquid be aqueous solution, the second liquid be with water not The waterborne liquid to dissolve each other, such as the first liquid are dextran solution, and second liquid is polyethylene glycol (PEG) aqueous solution, generation Drop is dextran solution drop.
First liquid and second liquid may be two kinds of liquid with interfacial reaction, in a reality of the invention It applies in example, first liquid is Na-alginate aqueous solution, and the second liquid is calcium oxide aqueous solution, as mass concentration is 1% calcium oxide aqueous solution, the two are calcium alginate gel bead there are interfacial reaction, the drop of generation.The application can be with Liquid pipette tips are spat by replacement or spit the component for flowing out the first liquid in liquid pipette tips, and multiple and different groups are sequentially formed in open containers Divide the drop with volume, both can be used to implement large batch of micro-volume high flux screening, the ultra micro of multi-step also may be implemented Biochemical reaction and detection are measured, is had broad application prospects.
The fluid driving mechanism 120 is connect with the arrival end for spitting liquid pipette tips 110, described spits liquid for that will be stored in First liquid inside pipette tips 110 is discharged from the outlet end for spitting liquid pipette tips 110.The motion control mechanism 130 is used Spit described in the control generated between the outlet end of liquid pipette tips 110 and the second liquid setting track or setting speed or setting plus The relative motion of speed, so that the first liquid of the outlet end for spitting liquid pipette tips 110 described in discharge overcomes surface tension and described spits Liquid pipette tips 110 form microlayer model to its adhesive force.First controller 170 respectively with the fluid driving mechanism 120 with And the motion control mechanism 130 connects, to control the fluid driving mechanism 120 and the motion control mechanism 130 Co-ordination.
There is document report microlayer model generation technique, such as membrane emulsification, micro-fluidic chip method, spits liquid rifle at spraying emulsion process Head injection/gunite etc..Wherein, liquid pipette tips injection/gunite is spat as newest microlayer model generation technique, in the life of microlayer model Good application prospect is all had at aspect and consumables cost control aspect.General liquid pipette tips injection/gunite needs of spitting are spat Liquid pipette tips above and below liquid level cutting movement to generate microlayer model.But this method can form unstable standing wave, micro- liquid in liquid level The generating process of drop is unstable.
Based on this, it is necessary to which to spit microlayer model generating process existing for liquid pipette tips injection/gunite unstable for general The problem of, a kind of microlayer model generation method that microlayer model generating process is stable is provided.
As shown in figure 3, in an embodiment of the present invention, under the drive of motion control mechanism 130, spitting liquid pipette tips 110 Outlet end 112 can be the movement comprising instantaneously accelerating, acceleration magnitude a under second liquid liquid level1.First liquid is from spitting The outlet end 112 of liquid pipette tips 110 forms the drop 195 for being attached to the outlet end 112 for spitting liquid pipette tips 110 after being discharged.Drop 195 exists Spitting the moment that the outlet ends 112 of liquid pipette tips 110 instantaneously accelerates is detached from and spits the outlet ends 112 of liquid pipette tips 110 and form microlayer model.Micro- liquid Dripping in the suffered active force being detached from before spitting the outlet end 112 of liquid pipette tips 110 is respectively the buoyancy of gravity G, second liquid f1, second liquid viscosity resistance f2And spit maximum adhesion power f between the outlet end 112 of liquid pipette tips 110 and drop 1953。 Microlayer model be detached from spit the outlet end 112 of liquid pipette tips 110 before quality be m, acceleration magnitude a2.It is transported according to newton second Dynamic law, obtains
Spit the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 1953With the table for spitting liquid pipette tips 110 Face free energy, drop 195 surface tension and spit liquid pipette tips 110 geometric dimension it is related.Spit the outlet end of liquid pipette tips 110 112 when doing instantaneous accelerated motion, spits the outlet end 112 of liquid pipette tips 110 to the direction of 195 adhesive force of drop and the direction of acceleration It is identical.The drop 195 for being attached to the outlet end 112 for spitting liquid pipette tips 110 is reduced to spherical.By Stokes (Stokes) formula It is found that the viscosity resistance f that drop 195 is suffered when moving in second liquid2=6 π η rv, wherein η is the viscous of second liquid Coefficient, r are the radius of drop 195, and v is the movement velocity of drop 195.Instantaneous acceleration is done in the outlet end 112 for spitting liquid pipette tips 110 The speed of drop 195 is zero before, therefore the moment that drop 195 instantaneously accelerates in the outlet end 112 for spitting liquid pipette tips 110 is second The viscosity resistance f being subject in liquid2It is zero or minimum.During microlayer model generates, the diameter range of general drop 195 exists Picoliters to microlitre the order of magnitude, and the buoyancy f of the gravity G of drop 195 and second liquid1It is contrary, therefore the weight of drop 195 The buoyancy f of power G and second liquid1Vector sum be about zero.Due to viscosity resistance f2It is zero or minimum and gravity G and buoyancy f1 Vector sum be about zero, soBy Newton interpolation algorithm it is found that spitting liquid pipette tips 110 When instantaneous accelerated motion is done in outlet end 112, the attainable peak acceleration in second liquid of drop 195 is a2≈f3/ m, wherein M is the quality of drop 195.As the acceleration a of drop 1952Less than the acceleration a for the outlet end 112 for spitting liquid pipette tips 1101When, liquid Drop 195 falls to form microlayer model from the outlet end 112 for spitting liquid pipette tips 110.Therefore, drop 195 is detached from the outlet for spitting liquid pipette tips 110 The condition at end 112 (generate a microlayer models) is approximately：a2≈(f3/ m) < a1。
Motion control mechanism 130 can accurately control the size for spitting 112 instantaneous acceleration of outlet end of liquid pipette tips 110.Cause This, it is larger by the value for controlling each instantaneous acceleration in outlet end 112 for spitting liquid pipette tips 110, spit the outlet end of liquid pipette tips 110 112, which do instantaneous accelerate, can effectively generate drop 195.
Based on this, the present invention also provides a kind of microlayer model generation methods, include the following steps：
S201 is provided and is spat liquid pipette tips 110 with outlet end 112, spits and store the first liquid in liquid pipette tips 110；It provides The microlayer model container of second liquid is stored, microlayer model container has opening, wherein the first liquid and second liquid are arbitrarily mutually Two kinds of immiscible liquid or two kinds of liquid with interfacial reaction；
S202 is spat under liquid level of the outlet end 112 of liquid pipette tips 110 by the opening insertion second liquid of microlayer model container；
The movement comprising instantaneously accelerating is done in S203, the outlet end 112 for spitting liquid pipette tips 110 under second liquid liquid level, simultaneously First liquid is discharged by the outlet end 112 for spitting liquid pipette tips 110, and the first liquid that the outlet end 112 for spitting liquid pipette tips 110 is discharged is formed It is attached to the drop 195 for spitting the outlet end 112 of liquid pipette tips 110, drop 195 adds in the instantaneous of the outlet end 112 for spitting liquid pipette tips 110 The outlet end 112 that liquid pipette tips 110 are spat in disengaging in fast motion process forms microlayer model under second liquid liquid level.
In above-mentioned microlayer model generation method, since the outlet end 112 for spitting liquid pipette tips 110 instantaneously accelerates brief acceleration number Be worth it is larger, be attached to the outlet end 112 for spitting liquid pipette tips 110 drop 195 and the outlet end 112 for spitting liquid pipette tips 110 Between adhesive force be not enough to drive drop 195 and 112 sync plus white of outlet end for spitting liquid pipette tips 110 so that attached Drop 195 in the outlet end 112 for spitting liquid pipette tips 110 be detached from and described spit the outlet end 112 of liquid pipette tips 110 in the second liquid Body fluid forms microlayer model under face.Microlayer model generation method provided by the present invention, the outlet end 112 for spitting liquid pipette tips 110 exist Microlayer model is generated when doing instantaneous accelerated motion under the liquid level of second liquid, the outlet end 112 for spitting liquid pipette tips 110 is reduced and transports It is disturbed caused by second liquid when dynamic, ensure that the stability of microlayer model generating process.
Optionally, in step S203, the first liquid can be by the mode that the outlet end 112 for spitting liquid pipette tips 110 is discharged Continuous discharge or discontinuous discharge.Specific discharge mode, can be designed accordingly according to actual condition.In the present embodiment In, in step S203, the first liquid is continuously discharged by the outlet end 112 for spitting liquid pipette tips 110, spits liquid pipette tips to make full use of The instantaneous each time of 110 outlet end 112 accelerates to generate microlayer model.In one embodiment, in step S203, the first liquid It is discharged, is implied that in equal time interval with constant flow velocity by spitting the outlet end 112 of liquid pipette tips 110, liquid pipette tips are spat in discharge First liquid volume of 110 outlet end 112 is always equal.First liquid is by spitting the outlet end 112 of liquid pipette tips 110 with constant Flow velocity discharge, be conducive to by control spit liquid pipette tips 110 outlet end 112 movement realize microlayer model generate control.
In an embodiment of the present invention, in step S203, the outlet end 112 of liquid pipette tips 110 is spat under second liquid liquid level Do the periodic motion comprising instantaneously accelerating.Periodic motion is done in the outlet end 112 for spitting liquid pipette tips 110 under second liquid liquid level, meaning Displacement, speed and the acceleration for spitting the outlet end 112 of liquid pipette tips 110 show periodic variation.Spit liquid pipette tips 110 Outlet end 112 is done comprising the periodic motion that instantaneously accelerates, the first liquid of cooperation by spit the outlet end 112 of liquid pipette tips 110 with Constant flow velocity discharge, the constant duration for realizing microlayer model generate.Or first liquid discharge spit the outlets of liquid pipette tips 110 The flow velocity at end 112 is variation, but within a period of motion of the outlet end 112 for spitting liquid pipette tips 110, and the discharge of the first liquid is spat The volume of the outlet end 112 of liquid pipette tips 110 keeps identical.Guarantee that the outlet end 112 for spitting liquid pipette tips 110 every time instantaneously accelerates with this The volume of preceding drop 195 be it is identical, to generate volume microlayer model of the same size.
Be changed without spit liquid pipette tips 110 and the first liquid in the case where, spit the surface free energy of liquid pipette tips 110, spit liquid rifle First 110 geometric dimension and the surface tension of drop 195 are spat between the outlet end 112 of liquid pipette tips 110 and drop 195 as influence Maximum adhesion power f3Two factors be determining.Therefore, be changed without spit liquid pipette tips 110 and the first liquid in the case where, spit The maximum value f of adhesive force between the outlet end 112 and drop 195 of liquid pipette tips 1103It is fixed.In fluid driving mechanism 120 Under drive, the first liquid can be realized the outlet end 112 for being continuously discharged with uniform flow velocity and spitting liquid pipette tips 110.Motion control machine Structure 130, which can be controlled accurately, to be spat the outlet ends 112 of liquid pipette tips 110 and is instantaneous acceleration a1At the time of movement and instantaneous acceleration a1 Size.Fluid driving mechanism 120 and the mutual cooperation of motion control mechanism 130 can readily realize the volume when drop 195 Reach the moment of fixed value, it is a that the outlet end 112 that liquid pipette tips 110 are spat in driving, which generates acceleration,1Instantaneous acceleration, with generate Volume microlayer model of the same size.If fluid driving mechanism 120 controls, the first liquid is uniform, continuously liquid pipette tips are spat in discharge 110 outlet end 112 only needs motion control mechanism 130 that the outlet end 112 for spitting liquid pipette tips 110 is driven to generate constant duration It is instantaneous to accelerate, that is, produce volume microlayer model of the same size.
When using it is multiple spit liquid pipette tips 110 simultaneously or sequentially generate microlayer model when, spit the surface free energy of liquid pipette tips 110 And the geometric dimensions of liquid pipette tips 110 is spat as influencing to spit maximum adhesion power between the outlet end 112 of liquid pipette tips 110 and drop 195 f3Two factors be variation.But batch machining can control the surface free energy for spitting liquid pipette tips 110 and spit liquid pipette tips 110 Geometric dimension changes in certain section.The surface tension of drop 195 as influence to spit the outlet ends 112 of liquid pipette tips 110 with Maximum adhesion power f between drop 1953Another factor also only change to a very small extent.Therefore, liquid pipette tips 110 are spat Outlet end 112 and drop 195 between adhesive force maximum value f3Only fluctuated in the section of very little.Fluid driving mechanism 120 It can drive the first liquid that the outlet end 112 for spitting liquid pipette tips 110 is continuously discharged with uniform flow velocity.130 energy of motion control mechanism It is instantaneous acceleration a in the outlet end 112 that liquid pipette tips 110 are spat in enough accurate control1At the time of movement and instantaneous acceleration a1Size. Fluid driving mechanism 120 can be realized readily with the mutual cooperation of motion control mechanism 130 when the volume of drop 195 reaches fixed The moment of value, it is a that the outlet end 112 that liquid pipette tips 110 are spat in driving, which generates acceleration,1Instantaneous acceleration, to generate volume size Consistent microlayer model.If fluid driving mechanism 120 controls the outlet that the first liquid is uniform, liquid pipette tips 110 are spat in continuous discharge End 112, the instantaneous acceleration fortune for only needing motion control mechanism 130 that the outlet end 112 for spitting liquid pipette tips 110 is driven to generate constant duration It is dynamic, that is, produce volume microlayer model of the same size.
Fluid driving mechanism 120 is while being at the uniform velocity discharged outlet end 112 for spitting liquid pipette tips 110 for the first liquid, cooperation Motion control mechanism 130 does the biggish instantaneous accelerated motion of acceleration value in the moment that the volume of drop 195 reaches setting value.This The microlayer model generation method that invention provides not only ensure that spitting liquid pipette tips 110 using same root generates the uniform drop of volume 195, while can guarantee that more are spat liquid pipette tips 110 simultaneously or the homogeneity of microlayer model volume size that sequentially generates.This reality The microlayer model generation method of example offer is applied while guaranteeing microlayer model volume size homogeneity, liquid pipette tips can be spat by more 110 generate the formation efficiency that microlayer model improves microlayer model simultaneously.
Further, under the control of motion control mechanism 130, the outlet end 112 of liquid pipette tips 110 is spat in a cycle It is accelerated in movement including repeatedly instantaneous, the acceleration magnitude repeatedly instantaneously accelerated is identical, and repeatedly instantaneously accelerates fortune Divide equally a period of motion for spitting the outlet end 112 of liquid pipette tips 110 at the time of dynamic.The outlet end 112 of liquid pipette tips 110 is spat at one It include repeatedly that instantaneous accelerate facilitated in the outlet end 112 for spitting liquid pipette tips 110 in a period of motion in cycle movement It is interior to generate multiple microlayer models.Optionally, in step S203, the outlet end 112 of liquid pipette tips 110 is spat in the subsurface fortune of second liquid Dynamic rail mark includes one of a variety of tracks such as straightway, arc section, polygon or a variety of combinations.It is achievable as one Mode spits liquid rifle when the outlet end 112 for spitting liquid pipette tips 110 includes instantaneous twice accelerate in a cycle movement First 110 motion profile is straight line or circular arc.When the outlet end 112 for spitting liquid pipette tips 110 includes in a cycle movement When instantaneous accelerated motion more than twice, it is regular polygon that track is done in the outlet end 112 for spitting liquid pipette tips 110 in second liquid, Including equilateral triangle, square, regular pentagon, regular hexagon etc..
As a kind of achievable mode, in step S203, the outlet end 112 of liquid pipette tips 110 is spat in second liquid liquid During periodic motion under face, the velocity magnitude for spitting the outlet end 112 of liquid pipette tips 110 changes in rectangular wave.Spit liquid pipette tips 110 Outlet end 112 velocity magnitude in rectangular wave change, boost phase terminate after i.e. enter constant velocity stage, be conducive to move control Mechanism 130 processed realizes the accurate control to the motion state for the outlet end 112 for spitting liquid pipette tips 110.Optionally, it indicates to spit liquid pipette tips The high-order time and low level time of the rectangular wave of 110 112 movement velocity size variation of outlet end can be it is equal can also be with It is different.Further, in step S203, the outlet end 112 of liquid pipette tips 110 is spat in the subsurface periodic motion of second liquid In the process, the velocity magnitude of the outlet end 112 of liquid pipette tips 110 is spat in square-wave variations.The outlet end 112 of liquid pipette tips 110 is spat in expression The high-order time and low level time of the rectangular wave of movement velocity size variation are equal.The outlet end of liquid pipette tips 110 is spat in expression When the rectangular wave of 112 movement velocity size variations is in low level, the speed for spitting the outlet end 112 of liquid pipette tips 110 is zero or has The speed of opposite direction when relative to a high position.As shown in figure 4, further, 112 period of outlet end for spitting liquid pipette tips 110 In the first half cycle of movement and later half period, the velocity magnitude of the outlet end 112 for spitting liquid pipette tips 110 is identical, contrary. It include instantaneous accelerated motion contrary twice within a period of motion of the outlet end 112 for spitting liquid pipette tips 110.
In the present embodiment, spitting the outlet ends 112 of liquid pipette tips 110 in the subsurface motion profile of second liquid is straight line Section, instantaneous accelerated motion is done from an endpoint of straightway in the outlet end 112 for spitting liquid pipette tips 110, from another end of straightway Point does the instantaneous accelerated motion of opposite direction.The acceleration magnitude instantaneously accelerated twice is a1.In other embodiments In, spitting the outlet ends 112 of liquid pipette tips 110 in the subsurface motion profile of second liquid is arc section or polygon.Further, In step S203, the frequency of the periodic motion under second liquid liquid level of outlet end 112 of liquid pipette tips 110 is spat between 0.1 hertz extremely It is easy to accomplish in engineering between 200 hertz.
As shown in Figures 4 and 5, in a specific embodiment of the invention, fluid driving mechanism 120 controls the first liquid The outlet end 112 of liquid pipette tips 110 is spat with constant flow velocity discharge.Motion control mechanism 130 controls the output end for spitting liquid pipette tips 110 Take exercises track be straight line, speed be in square-wave variations periodic motion.When the directional velocity for the outlet end 112 for spitting liquid pipette tips 110 When changing, the instantaneous acceleration for spitting the outlet end 112 of liquid pipette tips 110 reaches maximum value.It is attached to and spits going out for liquid pipette tips 110 Instantaneous acceleration of the drop 195 at mouth end 112 also in the outlet end 112 for spitting liquid pipette tips 110 is detached from when reaching maximum value and spits liquid rifle First 110 outlet end 112 and form microlayer model 199.Since the first liquid is the outlet for spitting liquid pipette tips 110 with constant flow rate discharge End 112, when drop 195 falls off from the outlet end 112 for spitting liquid pipette tips 110, new drop 195 enters generation state.When spitting liquid When the outlet end 112 of pipette tips 110 reversely accelerates again, newly-generated drop 195 also falls from the outlet end 112 for spitting liquid pipette tips 110 It falls to form new microlayer model 199.
In the present embodiment, spits and produces two microlayer models 199 in a period of motion of the outlet end 112 of liquid pipette tips 110, And square wave is easier to realize in engineering.In other examples, a period of motion of the outlet end 112 of liquid pipette tips 110 is spat One microlayer model 199 of interior generation.Optionally, the outlet ends 112 of liquid pipette tips 110 edge in second liquid 699 is spat in embodiment Any direction does the square wave that track is straight line and moves, including：Rail is done in the plane vertical with the extending direction for spitting liquid pipette tips 110 Mark be straight line square wave movement, with to spit the extending directions of liquid pipette tips 110 at track is done in plane at any angle be straight line The extending direction that liquid pipette tips 110 are spat in square wave movement, edge does the square wave that track is straight line and moves.In the other embodiment of the present invention In, when the motion profile for spitting the outlet end 112 of liquid pipette tips 110 is arc section or polygon, spit the outlet end 112 of liquid pipette tips 110 The square wave that track is straight line is done along any direction in second liquid 699 to move, including：With the extension side of spitting liquid pipette tips 110 Done into vertical plane the square wave that track is straight line move, with spit the extending directions of liquid pipette tips 110 at putting down at any angle The face track Nei Zuo is the square wave movement of straight line, does the square wave that track is straight line along the extending direction for spitting liquid pipette tips 110 and move.
Traditional spits liquid pipette tips generally in straight tube-like.Above-mentioned straight tube-like spit liquid pipette tips along itself extending direction close to go out When the one end at mouth end quickly moves, the microlayer model generated can be broken.It, need in order to keep the integrality of the microlayer model generated The vibration frequency for spitting liquid pipette tips is reduced, is reduced so as to cause the generating rate of microlayer model.
One embodiment of the invention provides and a kind of spits liquid pipette tips 110 for generate microlayer model 199 comprising has hollow The needle stalk 113 of cavity and the outlet end 112 for being set to 113 one end of needle stalk.Spit the normal of 112 end face of outlet end of liquid pipette tips 110 Angle between the extending direction of needle stalk 113 is less than or equal to 90 °.It shakes spitting extending direction of the liquid pipette tips 110 along pipe main body When dynamic, microlayer model 199 falls to fall behind in 699 viscous force of second liquid from the outlet end 112 for spitting liquid pipette tips 110 and spits liquid pipette tips 110 112 end face of outlet end squeezing action under far from outlet end 112 motion profile, avoid microlayer model 199 by outlet end 112 Break, maintain the integrality for having generated microlayer model 199, while it is fast along the extending direction of pipe main body to allow to spit liquid pipette tips 110 Speed vibration is to quickly generate microlayer model 199.
As shown in fig. 6, spitting liquid pipette tips 110 as a kind of achievable mode in straight tube-like, spitting the outlet of liquid pipette tips 110 End 112 is chamfered structure.The outlet end 112 for spitting liquid pipette tips 110 is chamfer, the integrality of generated microlayer model 199 is taken into account And microlayer model 199 formation efficiency while, have that structure is simple, be easily achieved, manufacturing cost is low, batch machining is with high accuracy Feature.Further, spit 112 end face of outlet end of liquid pipette tips 110 normal and needle stalk 113 extending direction between angle between Between 15 ° -75 °, the extension for spitting the normal and needle stalk 113 of 112 end face of outlet end of liquid pipette tips 110 can be designed according to actual condition Angle between direction.Spit the angle between the normal of 112 end face of outlet end of liquid pipette tips 110 and the extending direction of needle stalk 113 It should not be too large or too small, in order to avoid influence the generation of microlayer model 199 or break microlayer model 199.Further, liquid pipette tips 110 are spat 112 end face of outlet end normal and needle stalk 113 extending direction between angle between 30 ° -60 °.Specifically, spitting liquid Angle between the extending direction of normal and the needle stalk 113 of 112 end face of outlet end of pipette tips 110 is 45 °.45° angle can not only Guarantee being successfully generated for microlayer model 199, additionally it is possible to which generated 199 detachment of microlayer model is effectively opened to the movement of outlet end 112 Track avoids the outlet end for spitting liquid pipette tips 110 112 from breaking generated microlayer model 199.
As shown in fig. 7, needle stalk 113 is close to the outlet end 112 for spitting liquid pipette tips 110 as another achievable mode Part includes bending structure.The outlet end 112 for spitting liquid pipette tips 110 is bent, the integrality of generated microlayer model 199 is taken into account And microlayer model 199 formation efficiency while, have that structure is simple, be easily achieved, manufacturing cost is low, batch machining is with high accuracy Feature.Further, spit 112 end face of outlet end of liquid pipette tips 110 normal and needle stalk 113 extending direction between angle between Between 15 ° -75 °, the extension for spitting the normal and needle stalk 113 of 112 end face of outlet end of liquid pipette tips 110 can be designed according to actual condition Angle between direction.Spit the angle between the normal of 112 end face of outlet end of liquid pipette tips 110 and the extending direction of needle stalk 113 It should not be too large or too small, in order to avoid influence the generation of microlayer model 199 or break microlayer model 199.Further, liquid pipette tips 110 are spat 112 end face of outlet end normal and needle stalk 113 extending direction between angle between 30 ° -60 °.Specifically, spitting liquid Angle between the extending direction of normal and the needle stalk 113 of 112 end face of outlet end of pipette tips 110 is 45 °.45° angle can not only Guarantee being successfully generated for microlayer model 199, additionally it is possible to which generated 199 detachment of microlayer model is effectively opened to the movement of outlet end 112 Track avoids the outlet end for spitting liquid pipette tips 110 112 from breaking generated microlayer model 199.
Optionally, needle stalk 113 close to spit liquid pipette tips 110 outlet ends 112 bending structure have broken line, arc section, One of smooth curve section, straightway etc. or combination.As shown in fig. 7, in the present embodiment.Needle stalk 113 is close to spit liquid rifle The part of first 110 outlet end 112 has transition circle segmental arc, the specifically combination of arc section and straightway.In process It is easy to process by the circular arc bending of straight tube-like spat liquid pipette tips 110 and carry out set angle.
As can be seen from figures 8 and 9, it further includes pintle 114 that one embodiment of the invention provided, which spits liquid pipette tips 110, and pintle 114 has There is the reservoir 115 of the extending direction perforation pintle 114 along pintle 114.One end of reservoir 115 and needle stalk 113 are far from spitting liquid One end of the outlet end 112 of pipette tips 110 is connected to, and the one end of pintle 114 far from needle stalk 113 is the arrival end for spitting liquid pipette tips 110 111.It is fixedly connected between pintle 114 and needle stalk 113.The first liquid for generating microlayer model 199 can be stored in needle in advance In bolt 114, continuous, batch generation microlayer model 199 can be realized.Further, table in the one end of pintle 114 far from needle stalk 113 Face offers card slot 116.Card slot 116 can be realized to be detachably connected with fluid driving mechanism 120.Convenient for spitting liquid pipette tips 110 Replacement.
Also a kind of 199 generating means of microlayer model of the present invention, for generating microlayer model 199 under 699 liquid level of second liquid.It is micro- 199 generating means of drop include fluid driving mechanism 120, motion control mechanism 130 and above scheme is described in any item spits liquid Pipette tips 110.The internal reservoir for spitting liquid pipette tips 110 has the first liquid, spits liquid pipette tips 110 with outlet end 112 and arrival end 111. Fluid driving mechanism 120 is connect with the arrival end 111 for spitting liquid pipette tips 110, for that will be stored in spit inside liquid pipette tips 110 first Liquid is discharged from the outlet end 112 for spitting liquid pipette tips 110.Motion control mechanism 130 is for controlling the outlet end for spitting liquid pipette tips 110 112 generate setting track or setting speed under the liquid level of second liquid 699 or set the movement of acceleration, so that liquid is spat in discharge First liquid of the outlet end 112 of pipette tips 110 overcomes surface tension and adhesive force to form microlayer model 199 in second liquid 699.
It is provided by the invention to spit generation microlayer model 199 during liquid pipette tips 110 move under 699 liquid level of second liquid. As a kind of achievable mode, velocity magnitude is done in side in the outlet end 112 for spitting liquid pipette tips 110 under 699 liquid level of second liquid The movement of wave variation, acceleration magnitude a1.First liquid is formed after being discharged from the outlet end 112 for spitting liquid pipette tips 110 and is attached to Spit the drop 195 of the outlet end 112 of liquid pipette tips 110.The moment that drop 195 instantaneously accelerates in the outlet end 112 for spitting liquid pipette tips 110 It is detached from the formation of outlet end 112 microlayer model 199 for spitting liquid pipette tips 110.As shown in figure 3, microlayer model 199 spits liquid pipette tips 110 in disengaging Outlet end 112 before suffered active force be respectively gravity G, second liquid 699 buoyancy f1, second liquid 699 Viscosity resistance f2And spit maximum adhesion power f between the outlet end 112 of liquid pipette tips 110 and drop 1953.Microlayer model 199 is de- It is m from the quality before the outlet end 112 for spitting liquid pipette tips 110, acceleration magnitude a2.According to Newton interpolation algorithm, easily It obtains
Spit the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 1953With the table for spitting liquid pipette tips 110 Face free energy, drop 195 surface tension and spit liquid pipette tips 110 geometric dimension it is related.Spit the outlet end of liquid pipette tips 110 112 when doing instantaneous accelerated motion, spits the outlet end 112 of liquid pipette tips 110 to the direction of 195 adhesive force of drop and the direction of acceleration It is identical.The drop 195 for being attached to the outlet end 112 for spitting liquid pipette tips 110 is reduced to spherical.By Stokes (Stokes) formula It is found that the viscosity resistance f that drop 195 is suffered when moving in second liquid 6992=6 π η rv, wherein η is second liquid 699 The coefficient of viscosity, r be drop 195 radius, v be drop 195 movement velocity.Wink is done in the outlet end 112 for spitting liquid pipette tips 110 When accelerate before the speed of drop 195 be zero, therefore moment that drop 195 instantaneously accelerates in the outlet end 112 for spitting liquid pipette tips 110 The viscosity resistance f being subject in second liquid 6992It is zero or minimum.During microlayer model 199 generates, general drop 195 Diameter range picoliters to microlitre the order of magnitude, and the buoyancy f of the gravity G of drop 195 and second liquid 6991It is contrary, Therefore the buoyancy f of the gravity G of drop 195 and second liquid 6991Vector sum be about zero.ExistBy Newton interpolation algorithm it is found that instantaneous acceleration is done in the outlet end 112 for spitting liquid pipette tips 110 When movement, the attainable peak acceleration in second liquid 699 of drop 195 is a2≈f3/ m, wherein m is the matter of drop 195 Amount.Drop 195, which is detached from, to be spat the condition of the outlet end 112 (generate a microlayer model 199) of liquid pipette tips 110 and is approximately：a2≈(f3/ M) < a1。
Under the drive of motion control mechanism 130, the instantaneously acceleration of outlet end 112 for spitting liquid pipette tips 110 can be accurately controlled The size of degree.As long as the value for controlling each instantaneous acceleration in outlet end 112 for spitting liquid pipette tips 110 is larger, liquid pipette tips 110 are spat Instantaneous accelerate is done in outlet end 112 can effectively generate drop 195.Optionally, in the outlet end 112 for spitting liquid pipette tips 110 A period of motion in, form one or two or multiple microlayer models 199.
As shown in Figure 10, in an embodiment of the present invention, the normal and pipeline of 112 end face of outlet end of liquid pipette tips 110 are spat Angle between the extending direction of main body is 45 °, spits the outlet end 112 of liquid pipette tips 110 in chamfered structure.Second liquid 699 Liquid level upward, is spat liquid pipette tips 110 and is arranged vertically.Track is done under 699 liquid level of second liquid in the outlet end 112 for spitting liquid pipette tips 110 It is in the movement of square-wave variations for vertical line segment, velocity magnitude.Within a period of motion of the outlet end 112 for spitting liquid pipette tips 110 Generate a microlayer model 199.It spits and stores the first liquid in liquid pipette tips 110.Liquid pipette tips 110 are spat in the control of fluid driving mechanism 120 The first isometric liquid is discharged from outlet end 112 within each period of motion for spitting liquid pipette tips 110.Liquid pipette tips are spat when being attached to When the drop 195 of 110 outlet end 112 reaches setting volume size, the outlet end 112 of liquid pipette tips 110 is spat by upper limit position It is a with size1Acceleration instantaneously accelerate downwards, while be attached to spit the outlet end 112 of liquid pipette tips 110 drop 195 be detached from The outlet end 112 for spitting liquid pipette tips 110 forms microlayer model 199.In 699 viscous force of second liquid and spit the outlet ends of liquid pipette tips 110 Under the squeezing action of 112 end faces, motion profile of the microlayer model 199 far from outlet end 112 and close to spitting the side walls of liquid pipette tips 110. The outlet end 112 for spitting liquid pipette tips 110 continues to move downward, and the outlet for spitting liquid pipette tips 110 is still discharged in the first liquid at the same time End 112 forms the drop 195 for being attached to the outlet end 112 for spitting liquid pipette tips 110.When the outlet end 112 for spitting liquid pipette tips 110 moves to When lower limit position, the outlet end 112 for spitting liquid pipette tips 110 is moved upwards by lower limit position.In the outlet end for spitting liquid pipette tips 110 112 moved upwards by lower limit position during the first liquid the outlet end 112 for spitting liquid pipette tips 110 is still discharged, be attached to 195 volume of drop for spitting the outlet end 112 of liquid pipette tips 110 increases.When the outlet end 112 for spitting liquid pipette tips 110 moves to limes superiors When position, it is attached to the 195 volume size of drop for spitting the outlet end 112 of liquid pipette tips 110 and the last microlayer model 199 to fall off Volume is equal in magnitude.Spit the outlet ends 112 of liquid pipette tips 110 again by upper limit position with size for a1Acceleration it is instantaneous downwards Acceleration forms new microlayer model 199, so recycles.
As shown in figure 11, in an embodiment of the present invention, the normal and pipeline of 112 end face of outlet end of liquid pipette tips 110 are spat Angle between the extending direction of main body is 45 °, spits the outlet end 112 of liquid pipette tips 110 in chamfered structure.Second liquid 699 Liquid level upward, is spat liquid pipette tips 110 and is arranged vertically.Track is done under 699 liquid level of second liquid in the outlet end 112 for spitting liquid pipette tips 110 It is in the movement of square-wave variations for vertical line segment, velocity magnitude.Within a period of motion of the outlet end 112 for spitting liquid pipette tips 110 Generate two microlayer models 199.It spits and stores the first liquid in liquid pipette tips 110.Fluid driving mechanism 120 controls the first liquid with equal Uniform flow speed is discharged from outlet end 112.When the drop 195 for being attached to the outlet end 112 for spitting liquid pipette tips 110 reaches setting volume size When, spit the outlet ends 112 of liquid pipette tips 110 by upper limit position with size for a1Acceleration instantaneously accelerate downwards, adhere to simultaneously Drop 195 in the outlet end 112 for spitting liquid pipette tips 110 is detached from the formation of outlet end 112 microlayer model 199 for spitting liquid pipette tips 110.? It two liquid, 699 viscous force and spits under the squeezing action of 112 end face of outlet end of liquid pipette tips 110, microlayer model 199 is far from outlet end 112 motion profile and close to spitting the side walls of liquid pipette tips 110.Continue to move downward in the outlet end 112 for spitting liquid pipette tips 110.With this Simultaneously the first liquid still be discharged spit liquid pipette tips 110 outlet end 112 formation be attached to the outlet end 112 for spitting liquid pipette tips 110 Drop 195 is attached to 195 volume of the drop increase for spitting the outlet end 112 of liquid pipette tips 110.
When the outlet end 112 for spitting liquid pipette tips 110 moves to lower limit position, it is attached to the outlet end for spitting liquid pipette tips 110 112 195 volume size of drop and the volume for the microlayer model 199 that the last time falls off are equal in magnitude.Spit the outlet end of liquid pipette tips 110 112 by lower limit position be a with size1Acceleration instantaneously accelerate upwards, the drop 195 for being attached to outlet end 112 detaches Mouth end 112 forms new microlayer model 199.The outlet end 112 for spitting liquid pipette tips 110 is in the microlayer model generated when lower limit position 199 only move upwards a small distance under the action of the adhesive force of outlet end 112, just start in second liquid 699 gradually Landing.The first liquid, which is still discharged, during the outlet end 112 for spitting liquid pipette tips 110 is moved upwards by lower limit position spits liquid The outlet end 112 of pipette tips 110 is attached to 195 volume of the drop increase for spitting the outlet end 112 of liquid pipette tips 110.When spitting liquid pipette tips When 110 outlet end 112 moves to upper limit position, be attached to spit the outlet end 112 of liquid pipette tips 110 195 volume of drop it is big It is small equal in magnitude with the volume of the last microlayer model 199 to fall off.The outlet end 112 of liquid pipette tips 110 is spat again by limes superiors position It sets with size as a1Acceleration downwards instantaneously accelerate form new microlayer model 199, so recycle.When spit liquid pipette tips 110 go out When mouth end 112 is moved downward by upper limit position again, if still having micro- liquid in the footprint immediately below outlet end 112 Drop 199 then hits generated microlayer model 199 by the drop 195 for being attached to outlet end 112, and generated microlayer model 199 is along out The normal movement of mouth 112 end face of end is with the motion profile far from outlet end 112.
It is provided by the invention to spit generation microlayer model 199 during liquid pipette tips 110 move under 699 liquid level of second liquid. As another achievable mode, displacement is done in sine in the outlet end 112 for spitting liquid pipette tips 110 under 699 liquid level of second liquid The movement of variation.First liquid forms after being discharged from the outlet end 112 for spitting liquid pipette tips 110 and is attached to the outlet for spitting liquid pipette tips 110 The drop 195 at end 112.Drop 195 is detached from when 112 movement velocity of outlet end for spitting liquid pipette tips 110 reaches a certain size and spits liquid The outlet end 112 of pipette tips 110 forms microlayer model 199.As shown in fig. 6, microlayer model 199 is being detached from the outlet end for spitting liquid pipette tips 110 Suffered active force before 112 is respectively the buoyancy f of gravity G, second liquid 6991, second liquid 699 viscosity resistance f2 And spit maximum adhesion power f between the outlet end 112 of liquid pipette tips 110 and drop 1953.Microlayer model 199 spits liquid pipette tips in disengaging Quality before 110 outlet end 112 is m, speed v, acceleration a2.Motion process of the drop 195 in second liquid 699 In by viscous force f2, gravity G, buoyancy f1And adhesive force f3Collective effect, i.e.,Drop 195 are detached from and spit the condition of the outlet end 112 (generate a microlayer model 199) of liquid pipette tips 110 and be
Spit the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 1953With the table for spitting liquid pipette tips 110 Face free energy, drop 195 surface tension and spit liquid pipette tips 110 geometric dimension it is related.Liquid pipette tips 110 are spat by being attached to The drop 195 of outlet end 112 is reduced to spherical.By Stokes (Stokes) formula it is found that drop 195 is in second liquid 699 Suffered viscosity resistance f when middle movement2=6 π η rv, wherein η is the coefficient of viscosity of second liquid 699, and r is the half of drop 195 Diameter, v are the movement velocity of drop 195.During microlayer model 199 generates, the diameter range of general drop 195 picoliters extremely Microlitre the order of magnitude, and the coefficient of viscosity of second liquid 699 is generally bigger.Therefore generally haveAndTherefore, during spitting the outlet ends 112 of liquid pipette tips 110 and do speed change periodic motion under 699 liquid level of second liquid, Drop 195, which is detached from, to be spat the condition of the outlet end 112 (generate a microlayer model 199) of liquid pipette tips 110 and is approximatelyIt can Choosing, within a period of motion of the outlet end 112 for spitting liquid pipette tips 110, form one or two or multiple microlayer models 199.
As shown in figure 12, in an embodiment of the present invention, the normal and pipeline of 112 end face of outlet end of liquid pipette tips 110 are spat Angle between the extending direction of main body is 45 °, and needle stalk 113 is bending knot close to the part for the outlet end 112 for spitting liquid pipette tips 110 Structure.The liquid level of second liquid 699 upward, is spat liquid pipette tips 110 and is arranged vertically.The outlet end 112 of liquid pipette tips 110 is spat in second liquid Done under 699 liquid levels track be vertical line segment, displacement be in sinusoidal variations movement.At one of the outlet end 112 for spitting liquid pipette tips 110 A microlayer model 199 is generated in the period of motion.It spits and stores the first liquid in liquid pipette tips 110.The control of fluid driving mechanism 120 is spat The first isometric liquid is discharged in liquid pipette tips 110 within each period of motion for spitting liquid pipette tips 110 from outlet end 112.Spitting liquid It does displacement and generates first microlayer model in the acceleration decline stage of the linear motion of sinusoidal variations in the outlet end 112 of pipette tips 110 199.The initial stage of second microlayer model 199 is being generated, there are downward deceleration ranks although spitting the outlet ends 112 of liquid pipette tips 110 Section, but spit the 195 radius r of drop of the outlet end 112 of liquid pipette tips 110 due to being attached to and increase very fast, drop 195 is in second liquid Suffered viscosity resistance f when being moved in 6992There is no decline to show small-scale increase instead at once.Hereafter, drop 195 radius r are slowly increased, the viscosity resistance f suffered when moving in second liquid 699 of drop 1952Depending mainly on spitting liquid pipette tips The movement velocity of 110 outlet end 112 changes and changes.Spit liquid pipette tips 110 outlet end 112 drop to extreme position after start Rise, at the same time, is attached to and spits the volume of the drop 195 of outlet end 112 of liquid pipette tips 110 and constantly increase.
When the first liquid of control spits the outlet end 112 of liquid pipette tips 110 with even velocity of flow discharge, going out for liquid pipette tips 110 is spat Mouth end 112 generates and the equal bodies of a upper microlayer model 199 again at the time of generating a period of motion after a upper microlayer model 199 Long-pending new drop 195, and spit at this time liquid pipette tips 110 outlet end 112 movement velocity also with phase before a period of motion Together.Isometric new drop 195 falls off from the outlet end 112 for spitting liquid pipette tips 110 with a upper microlayer model 199, so recycles. Do the pendulum motion common guarantee that displacement is in sinusoidal variations in the outlet end 112 for being at the uniform velocity discharged and spitting liquid pipette tips 110 of first liquid Generate the volume size homogeneity of microlayer model 199.When the outlet end 112 for spitting liquid pipette tips 110 is downward by upper limit position again When movement, if microlayer model 199 is still had in the footprint immediately below outlet end 112, by being attached to outlet end 112 Drop 195 hits generated microlayer model 199, generated microlayer model 199 along 112 end face of outlet end normal movement with separate The motion profile of outlet end 112.
As shown in figure 13, in an embodiment of the present invention, the normal and pipeline of 112 end face of outlet end of liquid pipette tips 110 are spat Angle between the extending direction of main body is 45 °, and needle stalk 113 is bending knot close to the part for the outlet end 112 for spitting liquid pipette tips 110 Structure.The liquid level of second liquid 699 upward, is spat liquid pipette tips 110 and is arranged vertically.The outlet end 112 of liquid pipette tips 110 is spat in second liquid Done under 699 liquid levels track be vertical line segment, displacement be in sinusoidal variations movement.At one of the outlet end 112 for spitting liquid pipette tips 110 Two microlayer models 199 are generated in the period of motion.It spits and stores the first liquid in liquid pipette tips 110.The control of fluid driving mechanism 120 the One liquid is discharged with even velocity of flow from outlet end 112.With 195 radius of drop for being attached to the outlet end for spitting liquid pipette tips 110 112 R constantly increases, the viscosity resistance f suffered when moving in second liquid 699 of drop 1952Also constantly increase.When spitting liquid pipette tips When 110 outlet end 112 is in downward boost phase, the viscosity resistance suffered when being moved in second liquid 699 of drop 195 f2Greater than the maximum value f for spitting adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 1953, drop 195 is from spitting liquid pipette tips 110 Outlet end 112 fall off to form microlayer model 199.In 699 viscous force of second liquid and spit 112 end faces of outlet end of liquid pipette tips 110 Squeezing action under, motion profile of the microlayer model 199 far from outlet end 112 and close to spitting the side walls of liquid pipette tips 110.
The outlet end 112 for spitting liquid pipette tips 110 continues to move downward, and the outlet end 112 for spitting liquid pipette tips 110 drops to limit position It postpones and begins to ramp up.The first liquid, which is still discharged, at the same time spits the outlet ends 112 of liquid pipette tips 110 and is formed and be attached to and spit liquid pipette tips The drop 195 of 110 outlet end 112 is attached to 195 volume of the drop increase for spitting the outlet end 112 of liquid pipette tips 110.It is generating The initial stage of second microlayer model 199, when the movement velocity for the outlet end 112 for spitting liquid pipette tips 110 is reduced, but due to attached 195 radius r of drop in the outlet end 112 for spitting liquid pipette tips 110 increase it is very fast, when drop 195 moves in second liquid 699 Suffered viscosity resistance f2There is no decline to show small-scale increase instead at once.Hereafter, 195 radius r of drop is slow Increase, the viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Depending mainly on the outlet end for spitting liquid pipette tips 110 112 movement velocity changes and changes.
After the time interval of half period, the outlet end 112 for spitting liquid pipette tips 110 is in upward boost phase.It is attached to and spits The 195 volume size of drop of the outlet end 112 of liquid pipette tips 110 and the volume for the microlayer model 199 that the last time falls off are equal in magnitude, together When spit liquid pipette tips 110 outlet end 112 velocity magnitude it is also identical as before half period, be attached to the drop 195 of outlet end 112 It is detached from outlet end 112 and forms new microlayer model 199.The outlet end 112 of spitting liquid pipette tips 110 is in be generated when upward boost phase Microlayer model 199 only moves upwards a small distance under the action of the adhesive force of outlet end 112, just starts in second liquid 699 In gradually land.The first liquid, which is still discharged, at the same time spits the outlet ends 112 of liquid pipette tips 110 and is formed and be attached to and spit liquid pipette tips The drop 195 of 110 outlet end 112 is attached to 195 volume of the drop increase for spitting the outlet end 112 of liquid pipette tips 110.Half week After the time interval of phase, the outlet end 112 for spitting liquid pipette tips 110 is in downward boost phase.It is attached to the outlet for spitting liquid pipette tips 110 The 195 volume size of drop at end 112 and the volume for the microlayer model 199 that the last time falls off are equal in magnitude, while spitting liquid pipette tips 110 The velocity magnitude of outlet end 112 is also identical as before half period, and the drop 195 for being attached to outlet end 112 is detached from 112 shape of outlet end The microlayer model 199 of Cheng Xin so recycles.Control the outlet end 112 that the first liquid spits liquid pipette tips 110 with even velocity of flow discharge.It spits The outlet end 112 of liquid pipette tips 110 do track be vertical line segment, displacement in sinusoidal variations movement later half period boost phase After generating second microlayer model 199, into the stage for stably generating microlayer model 199.The at the uniform velocity discharge of first liquid and spit liquid rifle The volume size that the pendulum motion common guarantee that displacement is in sinusoidal variations generation microlayer model 199 is done in first 110 outlet end 112 is equal One property.When the outlet end 112 for spitting liquid pipette tips 110 is moved downward by upper limit position again, if immediately below outlet end 112 Microlayer model 199 is still had in footprint, then generated microlayer model is hit by the drop 195 for being attached to outlet end 112 199, generated microlayer model 199 along 112 end face of outlet end normal movement with far from outlet end 112 motion profile.
In the generating process of microlayer model 199, the first liquid spits the outlet end of liquid pipette tips 110 with the flow velocity discharge set 112.Spit liquid pipette tips 110 outlet end 112 do comprising instantaneously accelerate periodic motion when, microlayer model can not only be effectively generated 199, and convenient for controlling the size of generated microlayer model 199.Displacement is done in sine in the outlet end 112 for spitting liquid pipette tips 110 When the periodic motion of variation, microlayer model 199 can not be effectively generated, and generated microlayer model 199 has good volume Size homogeneity.During above two generation microlayer model 199, the first liquid is under the driving of fluid driving mechanism 120 The outlet end 112 of liquid pipette tips 110 is spat with the flow velocity discharge of setting.
As shown in Figure 14 and Figure 15, the present invention provides a kind of fluid driving mechanism 120, is used in microlayer model generation system, Including capacity component 121 and Power Component 122.Capacity component 121 includes injection tube 1211 and push rod 1212.Push rod 1212 It is slidably matched with the inner wall of injection tube 1211, driving liquid 1214 can be stored in injection tube 1211.Injection tube 1211 have into Liquid outlet 1213, liquid in-out mouth 1213 is for being connected to the arrival end 111 for spitting liquid pipette tips 110 for storing the first liquid 190.Power Component 122 and push rod 1212 are sequentially connected, and the extending direction for driving push rod 1212 along injection tube 1211 slides.In microlayer model In 199 generating process, the extruding of 122 driving push rod 1212 of Power Component, which is stored in injection tube 1211, drives liquid 1214, drives The extruding of hydrodynamic body 1214 is stored in the first liquid 190 spat in liquid pipette tips 110, and then by the first liquid 190 from spitting liquid pipette tips 110 Outlet end 112 be discharged.Fluid driving mechanism 120 provided by the invention utilizes the incompressible of liquid (driving liquid 1214) Property ensure that spit the outlet ends 112 of liquid pipette tips 110 remained in high-frequency vibration flow velocity according to setting by the first liquid 190 from The outlet end 112 for spitting liquid pipette tips 110 is discharged.Fluid driving mechanism 120 provided by the invention can accurately control generated micro- liquid Drip 199 volume sizes.Fluid driving mechanism 120 provided by the present invention is not limited to the above embodiment, for example, can also adopt With peristaltic pump, pressure-driven pump, pneumatic-driven pump or driven by electroosmosis pump etc..
As a kind of achievable mode, the liquid in-out mouth 1213 of injection tube 1211 and the arrival end 111 for spitting liquid pipette tips 110 Between be connected to by tubule 123.Driving liquid 1214 is stored in injection tube 1211 and in tubule 123.Power Component 122 and change The push rod 1212 of volume component 121 is sequentially connected, and Power Component 122 is for pushing the push rod 1212 of capacity component 121 infusing Sliding in shooting cylinder 1211.In the generating process of microlayer model 199, Power Component 122 pushes the push rod 1212 of capacity component 121, Push rod 1212 squeezes the driving liquid 1214 being stored in injection tube 1211 and tubule 123, and the driving extruding of liquid 1214 is stored in The first liquid 190 in liquid pipette tips 110 is spat, and then the first liquid 190 is discharged from the outlet end 112 for spitting liquid pipette tips 110.It uses The liquid in-out mouth 1213 of injection tube 1211 is connect by tubule 123 with the arrival end 111 for spitting liquid pipette tips 110, one side tubule 123 Internal diameter is smaller, convenient for realizing the accurate control to discharge liquid volume by the stroke of control push rod 1212；On the other hand, it uses Tubule 123 flexible arrangement injection tube 1211 and can spit the position between liquid pipette tips 110 and distance, convenient in 1211 He of injection tube It spits and arranges other necessaries between liquid pipette tips 110.
In an embodiment of the present invention, Power Component 122 pushes push rod 1212 at the uniform velocity to slide in injection tube 1211, implies that Liquid 1214 is driven to be discharged with uniform flow velocity from the liquid in-out mouth 1213 of capacity component 121 under the promotion of push rod 1212, Entered by tubule 123 with uniform flow velocity and spits liquid pipette tips 110.The first liquid 190 spat in liquid pipette tips 110 is stored in drive Under the promotion of liquid 1214, the outlet end 112 of liquid pipette tips 110 is spat with the discharge of uniform flow velocity.By using driving liquid 1214 It does driving medium and control push rod 1212 and driving liquid 1214, fluid driving provided in this embodiment is discharged with uniform flow velocity Mechanism 120 can not only when spitting liquid pipette tips 110 and remaining static with uniform flow velocity by the first liquid 190 by spitting liquid pipette tips 110 outlet end 112 is discharged.Even if spitting liquid pipette tips 110 to be under fast vibration state, fluid driver provided in this embodiment Structure 120 still ensures that the first liquid 190 from the outlet end 112 for spitting liquid pipette tips 110 with the discharge of uniform flow velocity.The present embodiment provides Fluid driving mechanism 120 substantially increase the volume size homogeneity of generated microlayer model 199.
The effect of Power Component 122 is to drive push rod 1212 in injection tube 1211 along the direction far from liquid in-out mouth 1213 Or it is slided close to the direction of liquid in-out mouth 1213.Optionally, Power Component 122 can be cylinder, hydraulic cylinder etc. and directly export The component of linear motion is also possible to convert circular motion to the component of linear motion, as motor and synchronous pulley combination, Motor and screw rod 1222 and the combination of sliding block 1223 etc..The present invention is not intended to limit the specific structure of Power Component 122.Such as Figure 15 institute Show, in an embodiment of the present invention, Power Component 122 includes driving motor 1221, screw rod 1222 and sliding block 1223.Driving motor 1221 output shaft and one end of screw rod 1222 are sequentially connected, and sliding block 1223 has internal screw thread, sliding block 1223 and 1222 table of screw rod The external screw thread in face is cooperatively connected.The outer rim of sliding block 1223 is fixedly connected with push rod 1212 far from one end of injection tube 1211.Sliding block 1223 convert sliding block 1223 along 1222 axial direction of screw rod for the rotary motion that driving motor 1221 exports with the cooperation of screw rod 1222 Linear motion, so that the push rod 1212 of capacity component 121 be driven to slide in injection tube 1211.Further, in the present embodiment The driving motor 1221 used is servo motor.Servo motor has the characteristics that accurate feedback and control angle displacement.
As shown in figure 16, in an embodiment of the present invention, fluid driving mechanism 120 further includes three-way diverter valve 124 and storage Flow container 125.Three-way diverter valve 124 has first interface, second interface and third interface.Spit liquid pipette tips 110 arrival end 111, The liquid in-out mouth 1213 and fluid reservoir 125 of capacity component 121 connect with the first interface of three-way diverter valve 124, second respectively Mouth and third orifice.Three-way diverter valve 124 at least can control fluid driving mechanism 120 and realize following two mode：One, The liquid in-out mouth 1213 of capacity component 121 is set to be connected with the arrival end 111 for spitting liquid pipette tips 110, in the band of Power Component 122 Under dynamic, capacity component 121 provides liquid driven power to liquid pipette tips 110 are spat, for that will spit the first liquid in liquid pipette tips 110 190 are discharged from the outlet end 112 for spitting liquid pipette tips 110, or the first liquid 190 is aspirated from the outlet end 112 for spitting liquid pipette tips 110 Into spitting in liquid pipette tips 110.Two, the liquid in-out mouth 1213 of capacity component 121 is made to be connected with fluid reservoir 125, in power packages Under the drive of part 122, the driving liquid 1214 in fluid reservoir 125 is drawn into capacity component 121 by capacity component 121 In injection tube 1211, or will be in the driving liquid push-in fluid reservoir 125 in capacity component 121.
As shown in figure 16, one embodiment of the invention also provides a kind of fluid driving method, using above-mentioned fluid driving mechanism, Include the following steps：(1) three-way diverter valve 124 is connected to the liquid in-out mouth 1213 of capacity component 121 with fluid reservoir 125.? Under the drive of Power Component 122, push rod 1212 changes note to one end sliding far from liquid in-out mouth 1213 in injection tube 1211 The volume of shooting cylinder 1211 sucks the driving liquid 1214 in fluid reservoir 125 in injection tube 1211.(2) three-way diverter valve 124 It is connected to the liquid in-out mouth 1213 of capacity component 121 with the arrival end 111 for spitting liquid pipette tips 110.In the drive of Power Component 122 Under, push rod 1212 slides the volume for changing injection tube 1211 in injection tube 1211 to one end close to liquid in-out mouth 1213, with In exhaustjet cylinder 1211, in tubule 123 and spit gas in liquid pipette tips 110.(3) outlet end 112 of liquid pipette tips 110 will be spat Into in the first liquid 190, and three-way diverter valve 124 is maintained to make the liquid in-out mouth 1213 of capacity component 121 and spit liquid pipette tips 110 arrival end 111 is connected to.Under the drive of Power Component 122, push rod 1212 is in injection tube 1211 to far from liquid in-out mouth 1213 one end sliding changes the volume of injection tube 1211, and 190 inspiration of the first liquid is spat in liquid pipette tips 110.(4) three are maintained Logical reversal valve 124 is connected to the liquid in-out mouth 1213 of capacity component 121 with the arrival end 111 for spitting liquid pipette tips 110.In power packages Under the drive of part 122, push rod 1212 at the uniform velocity slides change injection to one end close to liquid in-out mouth 1213 in injection tube 1211 The volume of cylinder 1211 will be stored in the first liquid 190 spat in liquid pipette tips 110 and spit liquid pipette tips 110 with the discharge of uniform flow velocity Outlet end 112.
For the ease of smoothly the gas in injection tube 1211 is discharged in above-mentioned second step, as shown in figure 15, peace Upward, push rod 1212 slides in injection tube 1211 the liquid in-out mouth 1213 of injection tube 1211 along the vertical direction when dress.
In order to improve the formation efficiency of microlayer model 199, as a kind of achievable mode, the quantity for spitting liquid pipette tips 110 is It is multiple, it is multiple to spit the setting of 110 spacing side by side of liquid pipette tips or arranged in the form of other.Each spitting liquid pipette tips 110 passes through list Only tubule 123 is connected to the first interface of three-way diverter valve 124.The quantity of capacity component 121 is one, capacity component 121 liquid in-out mouth 1213 is connected to the second interface of three-way diverter valve 124.The third interface and liquid storage of three-way diverter valve 124 Tank 125 is connected to.Under the driving of Power Component 122, push rod 1212 is in injection tube 1211 along the side close to liquid in-out mouth 1213 To at the uniform velocity sliding, while driving liquid 1214 being squeezed to multiple and is spat in liquid pipette tips 110.Due to being simultaneously between multiple tubules 123 The flow of connection relationship, the driving liquid 1214 in each tubule 123 is identical, ensure that multiple the first liquid spat in liquid pipette tips 110 Body 190 spits the outlet end 112 of liquid pipette tips 110 with identical, constant flow velocity discharge.And then it ensure that generated microlayer model 199 Volume size homogeneity.
In order to improve the formation efficiency of microlayer model 199, as another achievable mode, liquid pipette tips 110 and transfiguration are spat The quantity of product component 121 is multiple.It is multiple to spit the setting of 110 spacing side by side of liquid pipette tips or arranged in the form of other.Each Liquid pipette tips 110 are spat to be connected to by individual tubule 123 with the first interface of three-way diverter valve 124.Each capacity component 121 Liquid in-out mouth 1213 be connected to also by individual tubule 123 with the second interface of three-way diverter valve 124.Three-way diverter valve 124 Third interface be connected to fluid reservoir 125.Multiple 121 spacing side by side of capacity component settings are arranged in the form of other. The push rod 1212 of multiple capacity components 121 is relatively fixed far from one end of injection tube 1211, is synchronized and is pushed away by Power Component 122 It is dynamic.Under the driving of Power Component 122, multiple push rods 1212 are in respective injection tube 1211 along close to liquid in-out mouth 1213 Direction is at the uniform velocity slided, while driving liquid 1214 being squeezed to multiple and is spat in liquid pipette tips 110.Due to being between multiple tubules 123 The flow of parallel relationship, the driving liquid 1214 in each tubule 123 is identical, ensure that multiple first spat in liquid pipette tips 110 Liquid 190 spits the outlet end 112 of liquid pipette tips 110 with identical, constant flow velocity discharge.And then it ensure that generated microlayer model 199 Volume size homogeneity.
In order to improve the formation efficiency of microlayer model 199, as the third achievable mode, as shown in figure 17, liquid rifle is spat First 110, the quantity of capacity component 121 and three-way diverter valve 124 is identical and is multiple.Each spit the entrance of liquid pipette tips 110 End 111 is connected to the first interface of a three-way diverter valve 124 respectively by individual tubule 123.Each capacity component 121 Liquid in-out mouth 1213 be connected to respectively with the second interface of a three-way diverter valve 124 by individual tubule 123.Each threeway The third interface of reversal valve 124 is connected to fluid reservoir 125 respectively.Optionally, fluid reservoir 125 can be one or more.Often A the first liquid 190 spat in liquid pipette tips 110 can be the same or different.Multiple 121 spacing side by side of capacity component settings Or it is arranged in the form of other.The push rod 1212 of multiple capacity components 121 is relatively fixed far from one end of injection tube 1211, It is pushed by Power Component 122 is synchronous.Under the driving of Power Component 122, multiple push rods 1212 are in respective injection tube 1211 It is at the uniform velocity slided along the direction close to liquid in-out mouth 1213.A variety of different types of microlayer models 199 can be generated simultaneously.
In order to improve the formation efficiency of microlayer model 199, as the 4th kind of achievable mode, liquid pipette tips 110, capacity are spat The quantity of component 121 and three-way diverter valve 124 is identical and is multiple.The arrival end 111 for each spitting liquid pipette tips 110 passes through individually Tubule 123 be connected to respectively with the first interface of a three-way diverter valve 124.The liquid in-out mouth of each capacity component 121 1213 are connected to the second interface of a three-way diverter valve 124 respectively by individual tubule 123.Each three-way diverter valve 124 Third interface be connected to respectively with fluid reservoir 125.Optionally, fluid reservoir 125 can be one or more.Each spit liquid pipette tips The first liquid 190 in 110 can be the same or different.Multiple 121 spacing side by side of capacity component are arranged or with other Form arrangement.Each capacity component 121 respectively corresponds individual Power Component 122.Under the driving of Power Component 122, Multiple push rods 1212 at the uniform velocity slide in respective injection tube 1211 along the direction close to liquid in-out mouth 1213.It can not only be simultaneously A variety of different types of microlayer models 199 are generated, are guaranteeing that the volume size for each spitting the generated microlayer model 199 of liquid pipette tips 110 is equal One in advance under, additionally it is possible to control the volume size of every kind of drop 195 respectively.Convenient for multiple microlayer models for spitting liquid pipette tips 110 199 generation states are independently controlled.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed, But it should not be understood as limiting the scope of the patent of the utility model.It should be pointed out that for the common of this field For technical staff, without departing from the concept of the premise utility, various modifications and improvements can be made, these all belong to In the protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.

Claims (8)

1. a kind of fluid driving mechanism generates system for microlayer model, which is characterized in that including：
Capacity component, including injection tube and push rod, the inner wall of the push rod and the injection tube are slidably matched, the injection tube Interior to store driving liquid, the injection tube has liquid in-out mouth, and the liquid in-out mouth stores the first liquid for being connected to The arrival end for spitting liquid pipette tips；
Power Component is sequentially connected with the push rod, for driving the push rod to slide along the extending direction of the injection tube；
In the generating process of microlayer model, the Power Component drives the push rod to squeeze the driving being stored in the injection tube Liquid, driving liquid extruding are stored in the first liquid spat in liquid pipette tips, and then by the first liquid from the outlet end for spitting liquid pipette tips Discharge.
2. fluid driving mechanism according to claim 1, which is characterized in that the liquid in-out mouth of the injection tube with spit It is connected between the arrival end of liquid pipette tips by tubule.
3. fluid driving mechanism according to claim 1, which is characterized in that the Power Component can drive the push rod It is at the uniform velocity slided in the injection tube.
4. fluid driving mechanism according to claim 1, which is characterized in that further include：
Fluid reservoir, for storing driving liquid；
Three-way diverter valve has first interface, second interface and third interface, spits arrival end, the liquid in-out mouth of liquid pipette tips And the fluid reservoir respectively with the first interface, the second interface and the third orifice.
5. fluid driving mechanism according to claim 1, which is characterized in that the Power Component includes driving motor, silk Bar and sliding block, the output shaft of the driving motor are connect with the screw rod transmission, and the screw rod is threadedly coupled with the sliding block, institute Sliding block is stated to be fixedly connected with the push rod.
6. fluid driving mechanism according to claim 5, which is characterized in that the driving motor is servo motor.
7. fluid driving mechanism according to claim 1, which is characterized in that the quantity of the capacity component be it is multiple, The push rod of multiple capacity components is sequentially connected with the Power Component.
8. fluid driving mechanism according to claim 1, which is characterized in that the capacity component and the Power Component It is multiple and quantity is identical, multiple capacity component spacing side by side settings, each capacity component is by individual The Power Component driving.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110064451A (en) * 2018-01-24 2019-07-30 思纳福(北京)医疗科技有限公司 Fluid driving mechanism and fluid driving method
WO2019144894A1 (en) * 2018-01-24 2019-08-01 北京光阱管理咨询合伙企业（有限合伙） Motion control mechanism, liquid spitting pipette tip, micro-droplet generating device, fluid driving mechanism and fluid driving method, micro-droplet generating method, and surface treatment method for liquid spitting pipette tip
CN115518703A (en) * 2021-06-24 2022-12-27 北京致雨生物科技有限公司 Droplet generation device, system and method for generating droplets
US11946100B2 (en) 2018-01-24 2024-04-02 Sniper (Suzhou) Life Technology Co., Ltd. Microdroplet container and method for manufacturing the same, method for spreading microdroplets, microdroplet-generating kit, temperature-controlling device, oil phase composition for microdroplet generating and method for treating the same

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110064451A (en) * 2018-01-24 2019-07-30 思纳福(北京)医疗科技有限公司 Fluid driving mechanism and fluid driving method
WO2019144894A1 (en) * 2018-01-24 2019-08-01 北京光阱管理咨询合伙企业（有限合伙） Motion control mechanism, liquid spitting pipette tip, micro-droplet generating device, fluid driving mechanism and fluid driving method, micro-droplet generating method, and surface treatment method for liquid spitting pipette tip
US11666900B2 (en) 2018-01-24 2023-06-06 Sniper (Suzhou) Life Technology Co. Motion controlling mechanism, liquid discharging nozzle, microdroplet generating device and method, liquid driving mechanism and method, microdroplet generating method, and surface processing method of liquid discharging nozzle
CN110064451B (en) * 2018-01-24 2023-08-15 思纳福(苏州)生命科技有限公司 Fluid driving mechanism and fluid driving method
US11946100B2 (en) 2018-01-24 2024-04-02 Sniper (Suzhou) Life Technology Co., Ltd. Microdroplet container and method for manufacturing the same, method for spreading microdroplets, microdroplet-generating kit, temperature-controlling device, oil phase composition for microdroplet generating and method for treating the same
CN115518703A (en) * 2021-06-24 2022-12-27 北京致雨生物科技有限公司 Droplet generation device, system and method for generating droplets

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