CN110064444A - Microlayer model, which generates, uses oil phase composition and its processing method - Google Patents
Microlayer model, which generates, uses oil phase composition and its processing method Download PDFInfo
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- CN110064444A CN110064444A CN201810069265.5A CN201810069265A CN110064444A CN 110064444 A CN110064444 A CN 110064444A CN 201810069265 A CN201810069265 A CN 201810069265A CN 110064444 A CN110064444 A CN 110064444A
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- oil phase
- pipette tips
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- microlayer model
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Abstract
The present invention relates to a kind of microlayer model generation oil phase compositions, including mineral oil and surfactant.The percent by volume of mineral oil described in the oil phase composition is 88%-98.5%.The surfactant includes the silicon oxygen chain nonionic surface active agent containing chain-like alkyl.The invention further relates to a kind of processing methods for handling above-mentioned microlayer model generation oil phase composition.Above-mentioned microlayer model, which generates, uses oil phase composition, the density of mineral oil and the silicon oxygen chain nonionic surface active agent containing chain-like alkyl is smaller, declines in second liquid after can allow for the first liquid of most of type to be detached from the outlet end formation microlayer model for spitting liquid pipette tips.Silicon oxygen chain nonionic surface active agent containing chain-like alkyl can prevent from mutually merging between numerous microlayer models.
Description
Technical field
The present invention relates to the measurements of micro liquid, distribution technique field, generate more particularly to a kind of microlayer model with oily phase
Composition and its processing method.
Background technique
It examines at present in clinical medicine, nano material preparation, the application fields such as food and environment measuring, biochemical analysis have
To the widespread demand of micro liquid precise manipulation.The core technology of submicroliter fluid handling first is that the liquid of microlitre magnitude into
One step is divided into nanoliter micro- reaction system of even picoliters volume.The major technique branch that micro- reaction system generates is emulsification
Microlayer model generates.
In recent years, a variety of microlayer model generation techniques are reported in the literature, such as membrane emulsification, spraying emulsion process, miniflow
Control chip method, spit liquid pipette tips injection/gunite etc..Wherein, liquid pipette tips injection/gunite, which is spat, as newest microlayer model generates skill
Art all has good application prospect in terms of the generation of microlayer model and in terms of consumables cost control.Using spit liquid pipette tips injection/
When gunite, the outlet end for spitting liquid pipette tips generates microlayer model in the inside of oil phase composition.Traditional oil phase composition is using
In the process, the change in physical such as viscosity are bigger, and the volume size homogeneity of generated microlayer model is poor.
Summary of the invention
Based on this, it is necessary to traditional oil phase composition is used in liquid pipette tips injection/gunite for spitting, it is generated micro-
The poor problem of drop volume size homogeneity provides a kind of microlayer model generation that can guarantee microlayer model volume size homogeneity
With oil phase composition and its processing method.
A kind of microlayer model generation oil phase composition, including following components:
Mineral oil, the percent by volume of mineral oil described in the oil phase composition are 88%-98.5%；
Surfactant, the surfactant include the silicon oxygen chain nonionic surface active agent containing chain-like alkyl.
A kind of processing method of oil phase composition, for handling the described in any item oil phase compositions of above scheme；It is described
The processing method of oil phase composition includes heating the oil phase composition, while the oil phase composition is placed in negative pressure and ultrasound
In the environment of wave vibration.
The above-mentioned microlayer model including mineral oil and the silicon oxygen chain nonionic surface active agent containing chain alkyl is generated with oil
The density for being combined object is less than 1g/ml, and the outlet end that can allow for the first liquid disengaging of most of type to spit liquid pipette tips is formed
Decline in second liquid after microlayer model.Silicon oxygen chain nonionic surface active agent containing chain-like alkyl can prevent numerous micro- liquid
It is mutually merged between drop.
Detailed description of the invention
Fig. 1 is the overall structure diagram of digital pcr detector provided by the invention；
Fig. 2 is the microlayer model generating means of digital pcr detector provided by the invention；
Fig. 3 be another embodiment of the present invention provides spit liquid pipette tips outlet end motion when drop stress diagram；
Fig. 4 be the drop that provides of one embodiment of the invention with the outlet end motion for spitting liquid pipette tips when ideally viscous resistance
Power changes schematic diagram；
Fig. 5 is to generate a microlayer model two periods of motion of the outlet end for spitting liquid pipette tips that one embodiment of the invention provides
Process schematic；
Fig. 6 is to generate a microlayer model one period of motion of the outlet end for spitting liquid pipette tips that one embodiment of the invention provides
Process schematic；
Fig. 7 is to generate two microlayer models one period of motion of the outlet end for spitting liquid pipette tips that one embodiment of the invention provides
Process schematic；
Fig. 8 is the generating process schematic diagram for spitting microlayer model when liquid pipette tips are swung that one embodiment of the invention provides；
The generating process schematic diagram of microlayer model when Fig. 9 is the viscosity change for the second liquid that one embodiment of the invention provides；
Figure 10 is the generating process schematic diagram of microlayer model when liquid pipette tips are spat in the replacement that one embodiment of the invention provides；
Figure 11 is the outlet end for spitting liquid pipette tips that provides of one embodiment of the invention microlayer model under different motion profiles
Generating process schematic diagram；
Figure 12 be another embodiment of the present invention provides the outlet end velocity variations schematic diagram for spitting liquid pipette tips；
Figure 13 is the one experimental result distribution map of scheme that one embodiment of the invention provides；
Figure 14 is the high microlayer model enlarged drawing of the homogeneity for the generation of scheme one that one embodiment of the invention provides；
Figure 15 is the low microlayer model enlarged drawing of the homogeneity for the generation of scheme one that one embodiment of the invention provides；
Figure 16 is the two experimental result distribution schematic diagram of scheme that one embodiment of the invention provides；
Figure 17 is the high microlayer model enlarged drawing of the homogeneity for the generation of scheme two that one embodiment of the invention provides；
Figure 18 is the low microlayer model enlarged drawing of the homogeneity for the generation of scheme two that one embodiment of the invention provides；
Figure 19 is the high microlayer model enlarged drawing of the thermal stability for the generation of scheme two that one embodiment of the invention provides；
Figure 20 is the low microlayer model enlarged drawing of the thermal stability for the generation of scheme two that one embodiment of the invention provides；
Figure 21 is the microlayer model enlarged drawing that the scheme three that one embodiment of the invention provides generates；
Figure 22 is the high microlayer model enlarged drawing of the homogeneity for the generation of scheme four that one embodiment of the invention provides；
Figure 23 is the high microlayer model enlarged drawing of the thermal stability for the generation of scheme four that one embodiment of the invention provides；
Figure 24 is the flow control mechanism and spit liquid pipette tips connection schematic diagram that one embodiment of the invention provides；
Figure 25 is the flow control mechanism structural schematic diagram that one embodiment of the invention provides；
Figure 26 is that the liquid pipette tips of spitting that one embodiment of the invention provides generate the signal of microlayer model process in the case where driving liquid driven
Figure；
Figure 27 be another embodiment of the present invention provides flow control mechanism structural schematic diagram.
Wherein:
1- digital pcr detector；10- microlayer model generating means；20- temperature control device；30- fluorescence signal detection device；40-
Quantitative analysis device；50- controller；110- spits liquid pipette tips；The outlet end 112-；The first liquid of 190-；195- drop；The micro- liquid of 199-
Drop；120- fluid driving mechanism；121- capacity component；1211- injection tube；1212- push rod；1213- liquid in-out mouth；1214-
Drive liquid；122- Power Component；1221- driving motor；1222- screw rod；1223- sliding block；123- tubule；124- triplet commutation
Valve；125- fluid reservoir；130- motion control mechanism；The first controller of 170-；60- microlayer model container；699- second liquid；f1-
Buoyancy；F2- viscosity resistance；F3- maximum adhesion power；G- gravity.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, by the following examples, it and combines attached
Figure, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only to explain this hair
It is bright, it is not intended to limit the present invention.
It should be noted that it can directly on the other element when element is referred to as " being fixed on " another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to
To another element or it may be simultaneously present centering elements.On the contrary, when element is referred to as " directly existing " another element "upper",
There is no intermediary elements.Term as used herein "vertical", "horizontal", "left" and "right" and similar statement are
For illustrative purposes.Various difference objects are in the ratio drafting convenient for enumerating explanation in embodiment attached drawing, rather than press practical group
The ratio of part is drawn.
Digital pcr (Digital PCR, dPCR) is a kind of nucleic acid molecules absolute quantitation technology.Compared to qPCR, number
PCR can allow you that can directly count the number of DNA molecular, be the absolute quantitation to initial sample.Quantitative PCR is bent by standard
Line or reference gene measure nucleic acid amount, and digital pcr then allows you that can directly count the number of DNA molecular, be to starting sample
The absolute quantitation of product.
Referring to Figure 1, the present invention provides a kind of digital pcr detector 1, and digital pcr detector 1 includes: that microlayer model generates
Device 10, temperature control device 20, fluorescence signal detection device 30, quantitative analysis device 40 and controller 50.Microlayer model generates dress
10 are set to form multiple microlayer models for nucleic acid amplification reaction liquid droplet.Temperature control device 20 and microlayer model generating means 10 are logical
Track connection is crossed, multiple microlayer models are transferred to temperature control device 20, temperature cycles are carried out, realizes nucleic acid amplification.Fluorescence letter
Number detection device 30 is oppositely arranged with temperature control device 20, to carry out detection of taking pictures to multiple microlayer models after nucleic acid amplification.It is fixed
Amount analytical equipment 40 is connect with fluorescence signal detection device 30 by data line, to realize the biography of multiple microlayer model fluorescence informations
It is defeated, carry out quantitative analysis.Controller 50 respectively with microlayer model generating means 10, temperature control device 20, fluorescence signal detection device 30
And quantitative analysis device 40 connects, to control microlayer model generating means 10, temperature control device 20, fluorescence signal detection device 30
And quantitative analysis device 40.
Digital pcr detector 1 can by microlayer model generating means 10, temperature control device 20, fluorescence signal detection device 30 with
And quantitative analysis device 40 is integrated, so that automatic operation may be implemented in operator.Digital pcr detector 1 has
Higher working efficiency.
At work, microlayer model generating means 10 can carry out determined nucleic acid amplification reaction solution micro- digital pcr detector 1
Dropization, to form multiple microlayer models.Temperature control device 20 can carry out nucleic acid amplification to multiple microlayer models.Fluorescence signal detection dress
It sets 30 and claps the change in fluorescence picture for surveying multiple microlayer models in real time.It is available more by the change in fluorescence picture of multiple microlayer models
The change in fluorescence curve of a microlayer model.According to change in fluorescence curve, the Ct value of available multiple microlayer models, and by Ct value with
The relationship of starting copy number carries out quantitative analysis to the concentration of initial DNA.Wherein, Ct value refers to the fluorescence signal of each microlayer model
Reach recurring number experienced when the threshold value of setting.
Temperature control device 20 carries out nucleic acid amplification reaction to multiple microlayer models, and acquires core by fluorescence signal detection device 30
The product signal of multiple microlayer models after sour amplified reaction, such as fluorescence, UV absorption, turbidity signal.Using it is multiple amplification with
The difference of non-amplification microlayer model in composition, analyzes the amount of droplets for obtaining target sequence amplification, final to realize to core
The quantitative analysis of acid molecule.By the change in fluorescence picture of the multiple microlayer models of real-time monitoring, testing result has substantivity, can be with
Solve the problems, such as the false positive and false negative in multiple microlayer models.
Digital pcr detector 1 is by microlayer model generating means 10, temperature control device 20, fluorescence signal detection device 30 and determines
It is integrated to measure analytical equipment 40, so that automatic operation may be implemented in operator, not into improving work efficiency, also has anti-
Answer quick, reproducible, high sensitivity, high specificity and the clear advantage of result.
Fig. 2 is referred to, in one embodiment, microlayer model generating means 10 include spitting liquid pipette tips 110, fluid driving mechanism
120, motion control mechanism 130 and the first controller 170.Liquid pipette tips 110 are spat with outlet end and arrival end, and for storing
First liquid.Microlayer model generating means 10 can be used cooperatively with microlayer model container.Second liquid is stored in microlayer model container,
It spits under the liquid level of outlet end insertion second liquid of liquid pipette tips 110.
Between first liquid and second liquid it is immiscible or have interfacial reaction.First liquid and second liquid can be
Any immiscible two kinds of liquid, in one embodiment of the invention, the first liquid is aqueous solution, second liquid be with water not
The oil-based liquid to dissolve each other, such as mineral oil (including n-tetradecane), vegetable oil, silicone oil and perfluorine oil etc., the drop of generation
For aqueous solution droplets.Alternatively, the first liquid is mineral oil, such as the tetradecane and n-hexane organic phase, second liquid is and mineral
The immiscible perfluorine oil of oil.First liquid and second liquid can be immiscible aqueous two-phase, in another of the invention
In embodiment, the first liquid is aqueous solution, and it is dextran that second liquid, which is with the immiscible waterborne liquid of water, such as the first liquid,
Solution, second liquid are polyethylene glycol (PEG) aqueous solution, and the drop of generation is dextran solution drop.
First liquid and second liquid may be two kinds of liquid with interfacial reaction, in one embodiment of the present of invention
In, the first liquid be Na-alginate aqueous solution, second liquid is calcium oxide aqueous solution, as mass concentration be 1% calcium oxide water
Solution, the two are calcium alginate gel bead there are interfacial reaction, the drop of generation.The application can also spit liquid rifle by replacement
Head or the component for spitting the first liquid of outflow in liquid pipette tips, sequentially form the liquid of multiple and different components and volume in open containers
Drop, both can be used to implement large batch of micro-volume high flux screening, also may be implemented multi-step ultramicron biochemical reaction and
Detection, has broad application prospects.
Fluid driving mechanism 120 is connect with the arrival end for spitting liquid pipette tips 110, is spat inside liquid pipette tips 110 for that will be stored in
The first liquid from spit liquid pipette tips 110 outlet end be discharged.Motion control mechanism 130 is for controlling the outlet for spitting liquid pipette tips 110
Setting track or setting speed are generated between end and second liquid or sets the relative motion of acceleration, so that liquid pipette tips are spat in discharge
First liquid of 110 outlet end, which overcomes surface tension and spits liquid pipette tips 110, forms microlayer model to its adhesive force.First control
Device 170 is connect with fluid driving mechanism 120 and motion control mechanism 130 respectively, to control fluid driving mechanism 120 and
130 co-ordination of motion control mechanism.
In an embodiment of the present invention, under the drive of motion control mechanism 130, the outlet end 112 for spitting liquid pipette tips 110 exists
The movement that velocity magnitude is in mechanical periodicity is done under second liquid liquid level, first half cycle and later half period in velocity magnitude variation
Interior, the velocity magnitude for spitting the outlet end 112 of liquid pipette tips 110 is monotonically changed.Monotone variation refers to, in the first half of velocity magnitude variation
In period or later half period, the velocity amplitude at the rear moment of the outlet end 112 of liquid pipette tips 110 is spat always greater than being equal to or less than
Equal to the velocity amplitude at the preceding moment.For example, spitting the outlet end 112 of liquid pipette tips 110 in the first half cycle of velocity magnitude variation
Velocity magnitude continues to increase or segment section continues to increase and segment section is constant.Correspondingly, in the later half period of velocity magnitude variation
Interior, the velocity magnitude for spitting the outlet end 112 of liquid pipette tips 110 persistently reduces or segment section persistently reduces and segment section is constant.First
Liquid forms the drop 195 for being attached to the outlet end 112 for spitting liquid pipette tips 110 after being discharged from the outlet end 112 for spitting liquid pipette tips 110.
Drop 195 is detached from the outlet end for spitting liquid pipette tips 110 when 112 movement velocity of outlet end for spitting liquid pipette tips 110 reaches a certain size
112 form microlayer model 199.As shown in figure 3, microlayer model 199 before being detached from the outlet end 112 for spitting liquid pipette tips 110 suffered by
Active force be respectively gravity G, second liquid 699 buoyancy f1, second liquid 699 viscosity resistance f2And spit liquid pipette tips 110
Outlet end 112 and drop 195 between maximum adhesion power f3.Microlayer model 199 spits the outlet end 112 of liquid pipette tips 110 being detached from
Quality before is m, speed v, acceleration a2.Drop 195 is in the motion process of second liquid 699 by viscous force f2, again
Power G, buoyancy f1And adhesive force f3Collective effect, i.e.,Liquid pipette tips are spat in the disengaging of drop 195
The condition of 110 outlet end 112 (generate a microlayer model 199) is
Spit the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 1953With the table for spitting liquid pipette tips 110
Face free energy, drop 195 surface tension and spit liquid pipette tips 110 geometric dimension it is related.Liquid pipette tips 110 are spat by being attached to
The drop 195 of outlet end 112 is reduced to spherical.By Stokes (Stokes) formula it is found that drop 195 is in second liquid 699
Suffered viscosity resistance f when middle movement2=6 π η rv, wherein η is the coefficient of viscosity of second liquid 699, and r is the half of drop 195
Diameter, v are the movement velocity of drop 195.During microlayer model 199 generates, the diameter range of general drop 195 picoliters extremely
Microlitre the order of magnitude, and the coefficient of viscosity of second liquid 699 is generally bigger.Therefore generally haveAndTherefore, during spitting the outlet ends 112 of liquid pipette tips 110 and do speed change periodic motion under 699 liquid level of second liquid,
Drop 195, which is detached from, to be spat the condition of the outlet end 112 (generate a microlayer model 199) of liquid pipette tips 110 and is approximately
Based on this, the present invention provides a kind of microlayer model generation method, comprising the following steps:
S211 is provided and is spat liquid pipette tips 110 with outlet end 112, spits and store the first liquid in liquid pipette tips 110；It provides
The microlayer model container 60 of second liquid 699 is stored, microlayer model container 60 has opening；First liquid is with second liquid 699
Any two kinds of immiscible liquid or two kinds of liquid with interfacial reaction；
S212, the outlet end 112 for spitting liquid pipette tips 110 are inserted into the liquid level of second liquid 699 by the opening of microlayer model container 60
Under；
Velocity magnitude is done in mechanical periodicity in S213, the outlet end 112 for spitting liquid pipette tips 110 under 699 liquid level of second liquid
Movement, in the first half cycle and later half period of velocity magnitude variation, the velocity magnitude for spitting the outlet end 112 of liquid pipette tips 110 is equal
It is monotonically changed, while the first liquid is at the uniform velocity discharged by the outlet end 112 for spitting liquid pipette tips 110, the outlet end of liquid pipette tips 110 is spat in discharge
112 the first liquid forms the drop 195 for being attached to the outlet end 112 for spitting liquid pipette tips 110, and drop 195 is spitting liquid pipette tips 110
The outlet end 112 that liquid pipette tips 110 are spat in disengaging in the motion process of outlet end 112 forms microlayer model under 699 liquid level of second liquid
199。
Above-mentioned microlayer model generation method, spitting the outlet ends 112 of liquid pipette tips 110, to do speed under 699 liquid level of second liquid big
The small movement in mechanical periodicity spits the outlet end of liquid pipette tips 110 in the first half cycle and later half period of velocity magnitude variation
112 velocity magnitude is monotonically changed.In motion process, viscous force f of the second liquid 699 to drop 1952With spitting liquid pipette tips
The mechanical periodicity of 110 112 velocity magnitude of outlet end also shows mechanical periodicity.When the outlet end 112 and liquid for spitting liquid pipette tips 110
Maximum adhesion power f between drop 1953Less than second liquid 699 to the viscous force f of drop 1952When, drop 195 cannot with spit liquid
The outlet end 112 of pipette tips 110 moves synchronously, so be attached to spit the outlet end 112 of liquid pipette tips 110 drop 195 disengaging spit liquid
The outlet end 112 of pipette tips 110 forms microlayer model 199 under 699 liquid level of second liquid.Microlayer model generation side provided by the present invention
Speed change periodic motion is done under the liquid level of second liquid 699 to generate microlayer model 199 in method, the outlet end 112 for spitting liquid pipette tips 110,
To disturbance caused by second liquid 699 when reducing the movement of outlet end 112 for spitting liquid pipette tips 110, it ensure that microlayer model 199 generates
The stability of process.
In the present embodiment, in step S213, the first liquid is continuously discharged by the outlet end 112 for spitting liquid pipette tips 110.Into
One step, in step S213, the first liquid is discharged by spitting the outlet end 112 of liquid pipette tips 110 with constant flow velocity, is implied that equal
Time interval in, be discharged spit liquid pipette tips 110 outlet end 112 the first liquid volume it is always equal.First liquid is by spitting
The outlet end 112 of liquid pipette tips 110 is discharged with constant flow velocity, is conducive to the week by controlling the outlet end 112 for spitting liquid pipette tips 110
The movement of phase property, which is realized, generates volume microlayer model 199 of the same size.
Influence viscosity resistance f suffered when drop 195 moves in second liquid 6992Factor in, drop 195
Movement velocity v is easier to control.Before disengaging spits the outlet end 112 of liquid pipette tips 110 and forms microlayer model 199, drop 195
It keeps moving synchronously with the outlet end 112 for spitting liquid pipette tips 110.Therefore, the movement velocity v of drop 195 can spit liquid by control
The movement velocity of the outlet end 112 of pipette tips 110 realizes accurate control.It controls the first liquid and liquid pipette tips is spat with the discharge of uniform flow velocity
110 outlet end 112, the size r of 195 radius of drop be spaced at a fixed time in also show periodic variation.It influences
The viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Factor in, the coefficient of viscosity η of second liquid 699
It can change in a certain range in use, but the variation range very little of the coefficient of viscosity η of second liquid 699.
Be changed without spit liquid pipette tips 110 and the first liquid in the case where, spit the surface free energy of liquid pipette tips 110, spit liquid rifle
First 110 geometric dimension and the surface tension of drop 195 are spat between the outlet end 112 of liquid pipette tips 110 and drop 195 as influence
Maximum adhesion power f3Two factors be determining.Therefore, be changed without spit liquid pipette tips 110 and the first liquid in the case where, spit
The maximum value f of adhesive force between the outlet end 112 and drop 195 of liquid pipette tips 1103It is fixed.When spitting liquid pipette tips using multiple
110 simultaneously or sequentially generate microlayer model 199 when, spit the surface free energy of liquid pipette tips 110 and spit the geometric dimension of liquid pipette tips 110
Maximum adhesion power f between the outlet end 112 of liquid pipette tips 110 and drop 195 is spat as influence3Two factors be variation.But
Batch machining can control the surface free energy for spitting liquid pipette tips 110 and spit the geometric dimension of liquid pipette tips 110 in certain section
Variation.The surface tension of drop 195 spits maximum adhesion power f between the outlet end 112 of liquid pipette tips 110 and drop 195 as influence3
Another factor also only change to a very small extent.It spits and adheres between the outlet end 112 of liquid pipette tips 110 and drop 195
The maximum value f of power3Only fluctuated in the section of very little.
Therefore, viscosity resistance f suffered when drop 195 moves in second liquid 699 need to only be controlled2Greater than spitting liquid rifle
The maximum value f of adhesive force between first 110 outlet end 112 and drop 1953Interval value.Due to being generated in same batch
During microlayer model 199, the size r of 195 radius of drop should be fixed.Once experiment parameter determines, 195 radius of drop
Size r is also just determined therewith.It is variation that the outlet ends 112 of liquid pipette tips 110, which is spat, in the subsurface movement velocity of second liquid 699
's.When the outlet end 112 for spitting liquid pipette tips 110 meets v > f in the subsurface movement velocity of second liquid 6993When/6 π η r, drop
195 form microlayer model 199 from the disengaging of outlet end 112 for spitting liquid pipette tips 110.
The movement of velocity magnitude mechanical periodicity is done under 699 liquid level of second liquid in the outlet end 112 for spitting liquid pipette tips 110.Control
It makes the first liquid to be discharged with uniform flow velocity from the outlet end 112 for spitting liquid pipette tips 110, is attached to the outlet end for spitting liquid pipette tips 110
112 195 volume of drop is also uniformly to increase.First microlayer model 199 is fallen from the outlet end 112 for spitting liquid pipette tips 110
When, the radius of microlayer model 199 is known as critical radius, and the speed of microlayer model 199 becomes critical speed.Liquid pipette tips 110 are spat in adjustment
The flow velocity of the outlet end 112 of liquid pipette tips 110 is spat in the period of motion of outlet end 112 and the discharge of the first liquid, so that by identical
After time interval (multiple for spitting 112 period of motion of outlet end of liquid pipette tips 110), it is attached to the outlet end 112 for spitting liquid pipette tips 110
Drop 195 reach critical radius and critical speed simultaneously, the new formation of microlayer model 199.Since the first liquid is with uniform
The outlet end 112 for spitting liquid pipette tips 110 is discharged in flow velocity, and the volume size of microlayer model 199 generated is identical.
As a kind of achievable form, in step S213, in a velocity magnitude period of change, liquid pipette tips are spat
The velocity magnitude of 110 outlet end 112 is centrosymmetric using intermediate time point as midpoint.Further, in step S213, liquid is spat
The outlet end 112 of pipette tips 110 is in cyclically-varying in the subsurface acceleration of second liquid 699, speed and motion profile.More
Further, in step S213, the outlet ends 112 of liquid pipette tips 110 is spat in the subsurface velocity magnitude of second liquid 699 in cosine
Curvilinear motion.
Optionally, in step S213, the outlet end 112 of liquid pipette tips 110 is spat in the subsurface movement rail of second liquid 699
Mark includes one of a variety of tracks such as straightway, arc section, polygon or a variety of combinations.In step S213, liquid rifle is spat
The frequency of first 110 outlet end 112 periodic motion under 699 liquid level of second liquid between 0.1 hertz and 200 hertz,
It is easy to accomplish in engineering.
Track is done under 699 liquid level of second liquid and is become as circular arc, speed in cosine in outlet end 112 to spit liquid pipette tips 110
For the periodic motion of change, pendulum motion is actually done in the outlet end 112 for spitting liquid pipette tips 110 at this time, and moving displacement can be with just
Chord curve indicates, as shown in curve a in Fig. 4.Under the driving of flow control mechanism, the first liquid is with uniform flow velocity from spitting liquid
The outlet end 112 of pipette tips 110 is discharged.Assuming that drop 195 does not depart from the outlet end 112 for spitting liquid pipette tips 110.By calculating, drop
195 viscosity resistance f suffered when being moved in second liquid 699It is viscousIt changes over time as shown in curve b in Fig. 4.First liquid
With the initial stage that uniform flow velocity is discharged from the outlet end 112 for spitting liquid pipette tips 110, with the increase of 195 volume of drop, drop
195 radius r is also significantly increased.With the continuous increase of 195 radius r of drop, the at the uniform velocity increase of 195 volume of drop can only cause
The slow increase of 195 radius r of drop.Therefore, it spits in preceding several swing periods of the outlet end 112 of liquid pipette tips 110, drop 195
Suffered viscosity resistance f when being moved in second liquid 6992Maximum value increase sharply, then gradually tend to be slowly increased.
As shown in figure 4, the viscosity resistance f that drop 195 is suffered when moving in second liquid 6992Also show and spit liquid pipette tips 110
Outlet end 112 the similar periodicity of periodic motion, i.e., it is suffered viscous when drop 195 moves in second liquid 699
Resistance f2Change with the velocity variations for the outlet end 112 for spitting liquid pipette tips 110.In actual condition, when drop 195 is in the second liquid
Suffered viscosity resistance f when being moved in body 6992Increase and be greater than and spits between the outlet end 112 of liquid pipette tips 110 and drop 195
The maximum value f of adhesive force3When, drop 195 falls off to form microlayer model 199 from the outlet end 112 for spitting liquid pipette tips 110.
In an embodiment of the present invention, as shown in figure 5, control spit liquid pipette tips 110 outlet end 112 do track be circular arc,
Displacement is in the swing of sinusoidal variations.Be changed without spit liquid pipette tips 110 and the first liquid in the case where, spit the outlet of liquid pipette tips 110
The maximum value f of adhesive force between end 112 and drop 1953It is fixed.With being attached to the outlet end for spitting liquid pipette tips 110 112
195 radius r of drop constantly increases, the viscosity resistance f suffered when moving in second liquid 699 of drop 1952Also constantly increase
Greatly.The viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Greater than spit the outlet ends 112 of liquid pipette tips 110 with
The maximum value f of adhesive force between drop 1953Moment, drop 195 falls off to form micro- liquid from the outlet end 112 for spitting liquid pipette tips 110
Dripping is drop I in 199, Fig. 5.Into in the generation circulation of next round microlayer model 199.
In the present embodiment, the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 195 is spat3=1.8
×10-4N, the hunting frequency for spitting the outlet end 112 of liquid pipette tips 110 is 50 hertz.Position is done in the outlet end 112 for spitting liquid pipette tips 110
It moves and generates first microlayer model 199 in the second period end of the pendulum motion of sinusoidal variations, be drop I in Fig. 5.It is generating
The initial stage of second microlayer model 199, although the movement velocity for spitting the outlet end 112 of liquid pipette tips 110 is reduced, due to
It is attached to and spits the 195 radius r of drop of the outlet end 112 of liquid pipette tips 110 and increase very fast, drop 195 moves in second liquid 699
When suffered viscosity resistance f2There is no decline to show small-scale increase instead at once.Hereafter, 195 radius r of drop is slow
It is slow to increase, the viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Depending mainly on the outlet for spitting liquid pipette tips 110
The movement velocity at end 112 changes and changes.
When the first liquid of control spits the outlet end 112 of liquid pipette tips 110 with even velocity of flow discharge, going out for liquid pipette tips 110 is spat
Mouth end 112 generates and the equal bodies of a upper microlayer model 199 again at the time of generating two periods of motion after a upper microlayer model 199
Long-pending new drop 195 is drop II in Fig. 5.And spit at this time liquid pipette tips 110 outlet end 112 movement velocity also with two
It is identical before the period of motion.Isometric new drop 195 is from the outlet end 112 for spitting liquid pipette tips 110 with a upper microlayer model 199
It falls off.It is common in the pendulum motion of sinusoidal variations that displacement is done in the outlet end 112 for being at the uniform velocity discharged and spitting liquid pipette tips 110 of first liquid
It ensure that the volume size homogeneity for generating microlayer model 199.
In an embodiment of the present invention, as shown in fig. 6, control spit liquid pipette tips 110 outlet end 112 do track be circular arc,
Displacement is in the swing of sinusoidal variations.Be changed without spit liquid pipette tips 110 and the first liquid in the case where, spit the outlet of liquid pipette tips 110
The maximum value f of adhesive force between end 112 and drop 1953It is fixed.With being attached to the outlet end for spitting liquid pipette tips 110 112
195 radius r of drop constantly increases, the viscosity resistance f suffered when moving in second liquid 699 of drop 1952Also constantly increase
Greatly.The viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Greater than spit the outlet ends 112 of liquid pipette tips 110 with
The maximum value f of adhesive force between drop 1953Moment, drop 195 falls off to form micro- liquid from the outlet end 112 for spitting liquid pipette tips 110
Drop 199.Into in the generation circulation of next round microlayer model 199.
In the present embodiment, the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 195 is spat3=1.5
×10-4N, the hunting frequency for spitting the outlet end 112 of liquid pipette tips 110 is 50 hertz.Position is done in the outlet end 112 for spitting liquid pipette tips 110
It moves and generates first microlayer model 199 in a cycle end of the pendulum motion of sinusoidal variations, be drop I in Fig. 6.It is generating
The initial stage of second microlayer model 199, although the movement velocity for spitting the outlet end 112 of liquid pipette tips 110 is reduced, due to
It is attached to and spits the 195 radius r of drop of the outlet end 112 of liquid pipette tips 110 and increase very fast, drop 195 moves in second liquid 699
When suffered viscosity resistance f2There is no decline to show small-scale increase instead at once.Hereafter, 195 radius r of drop is slow
It is slow to increase, the viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Depending mainly on the outlet for spitting liquid pipette tips 110
The movement velocity at end 112 changes and changes.
When the first liquid of control spits the outlet end 112 of liquid pipette tips 110 with even velocity of flow discharge, going out for liquid pipette tips 110 is spat
Mouth end 112 generates and the equal bodies of a upper microlayer model 199 again at the time of generating a period of motion after a upper microlayer model 199
Long-pending new drop 195, and spit at this time liquid pipette tips 110 outlet end 112 movement velocity also with phase before a period of motion
Together.Isometric new drop 195 falls off from the outlet end 112 for spitting liquid pipette tips 110 with a upper microlayer model 199, is liquid in Fig. 6
Drip II.So circulation generates drop III, drop IV etc..The outlet end 112 for being at the uniform velocity discharged and spitting liquid pipette tips 110 of first liquid
Do the volume size homogeneity that the pendulum motion common guarantee that displacement is in sinusoidal variations generates microlayer model 199.
In an embodiment of the present invention, as shown in Figures 7 and 8, it does track and is in the outlet end 112 that liquid pipette tips 110 are spat in control
Circular arc, displacement are in the swing of sinusoidal variations.Be changed without spit liquid pipette tips 110 and the first liquid in the case where, spit liquid pipette tips 110
The maximum value f of adhesive force between outlet end 112 and drop 1953It is fixed.With being attached to the outlet end for spitting liquid pipette tips 110
112 195 radius r of drop constantly increases, the viscosity resistance f suffered when moving in second liquid 699 of drop 1952Also not
It is disconnected to increase.The viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Greater than the outlet end for spitting liquid pipette tips 110
The maximum value f of adhesive force between 112 and drop 1953Moment, drop 195 falls off shape from the outlet end 112 for spitting liquid pipette tips 110
It is drop I in Fig. 7 at microlayer model 199.Into in the generation circulation of next round microlayer model 199.
In the present embodiment, the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 195 is spat3=1.0
×10-4N, the hunting frequency for spitting the outlet end 112 of liquid pipette tips 110 is 50 hertz.Position is done in the outlet end 112 for spitting liquid pipette tips 110
The boost phase for moving the first half cycle of the pendulum motion in sinusoidal variations generates first microlayer model 199, is drop I in Fig. 7.?
The initial stage for generating second microlayer model 199, when the movement velocity for the outlet end 112 for spitting liquid pipette tips 110 is reduced, but by
Increase comparatively fast in the 195 radius r of drop for being attached to the outlet end 112 for spitting liquid pipette tips 110, drop 195 is transported in second liquid 699
Suffered viscosity resistance f when dynamic2There is no decline to show small-scale increase instead at once.Hereafter, 195 radius r of drop
It is slowly increased, the viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Depending mainly on spit liquid pipette tips 110 go out
The movement velocity at mouth end 112 changes and changes.
Control the outlet end 112 that the first liquid spits liquid pipette tips 110 with even velocity of flow discharge.Spit the outlet end of liquid pipette tips 110
112 generate second microlayer model 199 in the later half period boost phase for doing pendulum motion of the displacement in sinusoidal variations, are in Fig. 7
Drop II.Then into the stage for stably generating microlayer model 199.The outlet end 112 for spitting liquid pipette tips 110 generates second microlayer model
The new drop 195 isometric with second microlayer model 199 is generated at the time of half of period of motion after 199 again, and is spat at this time
The movement velocity of the outlet end 112 of liquid pipette tips 110 is also identical as before half of period of motion.With the equal bodies of second microlayer model 199
Long-pending new drop 195 falls off from the outlet end 112 for spitting liquid pipette tips 110, so recycles, generate drop III shown in fig. 7,
Drop IV, drop V etc..Do the pendulum that displacement is in sinusoidal variations in the outlet end 112 for being at the uniform velocity discharged and spitting liquid pipette tips 110 of first liquid
The dynamic volume size homogeneity for moving common guarantee and generating microlayer model 199.
It is detached from it can be seen from the above, being attached to and spitting the drop 195 of the outlet end 112 of liquid pipette tips 110 and spits the outlets of liquid pipette tips 110
The condition at end 112 (generating a microlayer model 199) is approximately:It is spat controlling the first liquid with even velocity of flow discharge
In the case where the outlet end 112 of liquid pipette tips 110, the uniform condition of volume of microlayer model 199 generated is: microlayer model
199 constant durations fall off from the outlet end 112 for spitting liquid pipette tips 110.
The maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 195 is spat in influence3Factor include: to spit
The surface tension of the surface free energies of liquid pipette tips 110, geometric dimension and the first liquid.Liquid pipette tips 110 and first are spat being changed without
In the case where liquid, the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 195 is spat3It is fixed.It influences
The viscosity resistance f suffered when being moved in second liquid 699 of drop 1952Factor include: second liquid 699 viscous system
Number η, the radius r of drop 195 and the movement velocity v of drop 195.The outlet end 112 for spitting liquid pipette tips 110 is at the uniform velocity discharged in first liquid
When, the radius r of drop 195 is determined by the interval time that microlayer model 199 generates.Drop 195 spits the outlet of liquid pipette tips 110 being detached from
It is moved synchronously before end 112 with the outlet end 112 for spitting liquid pipette tips 110, can realize that accurate control is spat by motion control mechanism 130
The movement velocity of the outlet end 112 of liquid pipette tips 110.Coefficient of viscosity η meeting in the generating process of drop 195 of second liquid 699
Change in a certain range, but the variation range very little of the coefficient of viscosity η of second liquid 699.As shown in figure 9, curve a expression is spat
The change in displacement of the outlet end 112 of liquid pipette tips 110, curve b and curve c are when the coefficient of viscosity η of second liquid 699 is in very little
The generating process curve of microlayer model 199 when changing in range.When the coefficient of viscosity η of second liquid 699 becomes to a very small extent
It, only can be at the generation moment of very a small range change microlayer model 199 when change.Between the generation time without changing microlayer model 199
Every.As shown in figure 9, the generation time interval of microlayer model 199 represented by curve b and curve c is half period t/2, guarantee
The volume size homogeneity of generated microlayer model 199.
As shown in Figure 10, when liquid pipette tips 110 are spat in replacement or temperature change etc. causes the surface tension of the first liquid to occur
When variation, the maximum value f of adhesive force between the outlet end 112 of liquid pipette tips 110 and drop 195 is spat3It is difficult to control accurately, therefore such as
199 volume of microlayer model that fruit generates is to f3Change in a certain range it is insensitive, then to generate single-size microlayer model 199
It is of great significance.In Figure 10, curve a indicates to spit the change in displacement of the outlet end 112 of liquid pipette tips 110, and curve b and curve c are
The generating process curve of microlayer model 199 in the case that liquid pipette tips 110 are spat in replacement.After liquid pipette tips 110 are spat in replacement, liquid pipette tips are spat
The maximum value f of adhesive force between 110 outlet end 112 and drop 1953Fluctuation will lead to drop 195 and fall off in a certain range
When spit the corresponding different speed in outlet end 112 of liquid pipette tips 110.But after the generation of microlayer model 199 reaches stable state, liquid
The speed for the outlet end 112 that drop 195 spits liquid pipette tips 110 when falling off is fixed in each swing period, as shown in Figure 10,
The generation time interval of microlayer model 199 represented by curve b and curve c is half period t/2.Therefore it can guarantee microlayer model
199 interval times generated were fixed.It is raw when the flow velocity that the outlet end 112 of liquid pipette tips 110 is spat in the discharge of the first liquid is fixed
At the volume of microlayer model 199 be uniform.The flow velocity that the outlet end 112 of liquid pipette tips 110 is spat in the discharge of the first liquid is adjusted simultaneously
And hunting frequency of the outlet end 112 of liquid pipette tips 110 in second liquid 699 is spat, uniform volume microlayer model can be controlled simultaneously
199 volume size and generating rate.
Spat in above-described embodiment liquid pipette tips 110 outlet end 112 do displacement be in sinusoidal variations periodic motion when, to attachment
The maximum value f of power3And viscosity resistance f2Variation have certain tolerance, i.e. the maximum value f of adhesive force3Or viscosity resistance f2
When changing in a certain range, it still is able to generate the uniform microlayer model 199 of volume.When the outlet end for spitting liquid pipette tips 110
112 do displacement be in sinusoidal variations periodic motion when, guarantee generate the uniform microlayer model 199 of volume under the premise of, can
The maximum value f of the adhesive force of tolerance3Variation range be known as plateau.The presence of plateau is for spitting the processing of liquid pipette tips 110
And the control that microlayer model 199 generates temperature has great importance.The presence of plateau, which allows to reduce to a certain extent, spits liquid
The requirement on machining accuracy of pipette tips 110, even if being had differences between batch surface free energy of processing spat between liquid pipette tips 110,
Also the uniform microlayer model 199 of volume can be generated.Similarly, the presence of plateau also allows to reduce micro- liquid to a certain extent
Drip the temperature control requirement of 199 generating process.
The presence of plateau allows to reduce the requirement on machining accuracy for spitting liquid pipette tips 110 or microlayer model 199 to a certain extent
The temperature control requirement of generating process further reduced consumables cost and control cost in 199 generating process of microlayer model.On
It states and generates two microlayer models 199 in each period of motion for spitting the outlet end 112 of liquid pipette tips 110 in embodiment, it is readily appreciated that
It is that only the periodic motion that displacement is in sinusoidal variations is done in the outlet end 112 of disgusting liquid pipette tips 110, when the outlet end for spitting liquid pipette tips 110
It is generated in 112 each period of motion and generates a microlayer model 199 in a microlayer model 199 or the every two period of motion
When, still to the maximum value f of adhesive force3And viscosity resistance f2Variation have certain tolerance, also all there is plateau.
Since the generation of microlayer model 199 is hardly influenced by the gravity and inertia force of microlayer model 199.Therefore micro- liquid is generated
When dripping 199, the outlet end 112 for spitting liquid pipette tips 110 can do the week that displacement is in sinusoidal variations in second liquid 699 along any direction
Phase movement.The motion profile for spitting the outlet end 112 of liquid pipette tips 110 is the track of camber line, straight line or other shapes.
As shown in (1) in Figure 11, in an embodiment of the present invention, the inclination insertion second liquid 699 of liquid pipette tips 110 is spat
Interior, the outlet end 112 for spitting liquid pipette tips 110 generates microlayer model 199 in 699 liquid level lower swing of second liquid.It can be achieved as one kind
Mode, as shown in (2) in Figure 11, it is horizontal straight that track is done in the outlet end 112 for spitting liquid pipette tips 110 in second liquid 699
Line, displacement are in the periodic motion of sinusoidal variations to generate microlayer model 199.As another achievable mode, in Figure 11
(3) shown in, it is vertical straight line, displacement in sinusoidal variations that track is done in second liquid 699 in the outlet end 112 for spitting liquid pipette tips 110
Periodic motion is to generate microlayer model 199.
As shown in figure 12, in an alternative embodiment of the invention, in step S213, a cycle of velocity magnitude variation
Interior, spitting the outlet ends 112 of liquid pipette tips 110 in first half cycle and later half period is uniform variable motion.Further, in step S213
In, the outlet end 112 for spitting liquid pipette tips 110 is equal with the acceleration magnitude in later half period in first half cycle.Control the first liquid with
The outlet end 112 for spitting liquid pipette tips 110 is discharged in even velocity of flow.With the continuous discharge of the first liquid, it is attached to and spits liquid pipette tips 110
The viscosity resistance f that the drop 195 of outlet end 112 is subject to during the motion2Also constantly increase.As viscosity resistance f2Greater than drop
195 and spit the maximum value f of adhesive force between liquid pipette tips 1103When, drop 195 forms microlayer model 199 from the disengaging of liquid pipette tips 110 is spat.
In the generating process for subsequently entering next microlayer model 199.The motion frequency and fortune of the outlet end 112 of liquid pipette tips 110 are spat in control
Dynamic speed is adapted with the flow velocity of the first liquid, to guarantee to generate the volume homogeneity of microlayer model 199.
The present invention provides a kind of microlayer model generation oil phase composition, i.e., above-mentioned second liquid 699, including with the following group
Point:
Mineral oil, the percent by volume of oil phase composition mineral oil in fluid are 88%-98.5%；
Surfactant, surfactant include the silicon oxygen chain nonionic surface active agent containing chain-like alkyl.
The above-mentioned microlayer model including mineral oil and the silicon oxygen chain nonionic surface active agent containing chain-like alkyl is generated with oil
The density for being combined object is less than 1g/ml, and the first liquid of most of type can allow for be detached from the outlet end for spitting liquid pipette tips 110
Decline in second liquid 699 after 112 formation microlayer models 199.Silicon oxygen chain nonionic surface active agent energy containing chain-like alkyl
It enough prevents from mutually merging between numerous microlayer models 199.
In an embodiment of the present invention, silicon oxygen chain nonionic surface active agent in oil phase composition containing chain-like alkyl
Percent by volume is 1.5%-12%.Further, the silicon oxygen chain nonionic surface active agent containing chain-like alkyl includesWithOne or both of.In one embodiment, surfactant further includes chain alkane
Hydrocarbon ester, the mass volume ratio of chain alkane esters and oil phase composition is 0.015g/mL-0.05g/mL in oil phase composition.Into one
Step, chain alkane esters include that dimerization hydroxy stearic acid ester (PEG-30), glycerol stearate, polyethylene glycol (30) dimerization hydroxyl are hard
One of resin acid ester (P135) etc. is a variety of.Specifically, chain alkane esters are polyethylene glycol (30) dimerization hydroxy stearic acid ester
(P135).In the present embodiment, the volume hundred of the silicon oxygen chain nonionic surface active agent in oil phase composition containing chain-like alkyl
Divide than being 1.5%-5.0%.In the above scheme, the silicon oxygen chain nonionic surface active agent containing chain-like alkyl is
In mineral oil, gas has a certain amount of solubility.It and is related with the temperature of gas and mineral oil.For example,
At room temperature, air can be dissolved in mineral oil, and be sightless.Mineral oil dissolved gas will affect the viscous of mineral oil
The physical properties such as degree, bulk modulus, heat transfer, boundary lubrication form foaming and cavitation phenomena.If the gas in mineral oil contains
Amount be more than saturation, it is seen that bubble just will form and be suspended in mineral oil, mineral oil will become very fuzzy.This, which is referred to as, presss from both sides
Band gas.Bubble rises to mineral oil meter face at leisure.In oil film, the bubble of gas will lead to the continuity of oil film, thus
Reduce oil film prevents the ability that other are in contact.Such as in PCR reaction process, temperature can be increased to 95 DEG C.In mineral oil
Gas solubility reduce, the gas content in mineral oil is higher than saturation value, at this moment just has bubble generation.Bubble can rise to
Mineral oil meter face, it is final to rupture.But bubble will affect the acquisition of fluorescence signal in this process.In addition, if when bubble generates
It interacts with microlayer model 199, will affect the stability of microlayer model 199, promote the fusion reaction between microlayer model 199.
The present invention also provides a kind of processing methods of oil phase composition, for handling the described in any item oily phases of above scheme
Composition.The processing method of oil phase composition includes heating oil phase composition, while oil phase composition is placed in negative pressure and ultrasound
In the environment of wave vibration.In subnormal ambient, make the air being dissolved in oil phase composition and other gas overflowings, utmostly
Meltage in oil phase composition of reduction air and other gases.Ultrasonic wave can promote to be dissolved in oil phase composition
The spilling of gas.When in microlayer model 199 including water phase, after generating microlayer model 199, microlayer model 199 is operated subsequent
When, it might have moisture and be dissolved in oil phase composition.So that 199 size of microlayer model is changed, will affect the position of microlayer model 199
Arrangement is set, and then influences the real-time detection to microlayer model 199.Further, it is also wrapped in the processing method of the oil phase composition
Including makes the oil phase composition reach water saturated step.Specifically, described make oil phase composition reach water saturated step packet
It includes: before heating oil phase composition, distilled water being added in oil phase composition.After the heating process of oil phase composition, oil
It is combined object natural cooling under 25 DEG C of -35 DEG C of environment.Distilled water is added in oil phase composition and is heated simultaneously, oil is combined
Object reaches water saturation.Remove undissolvable water at room temperature.Oil phase composition has been in water before generating microlayer model 199
Saturation state utmostly reduces the amount that the moisture in the microlayer model 199 of water phase enters oil phase composition.Further, make
With nitrogen protection oil phase composition after cooling.Nitrogen solubility in the oil phase composition based on mineral oil is very low.It uses
Nitrogen does protective gas, and air during storing oil phase composition in environment or other gases can be prevented because being dissolved in
The quality of oil phase composition is reduced in oil phase composition.As a kind of achievable mode, when the device of storage oil phase composition
When having remaining space in ware, full nitrogen will be filled in remaining space.
Scheme one: experimental exploring microlayer model 199 generated in the oil phase composition (second liquid 699) of different component
Volume size homogeneity.Week is done in oil phase composition (second liquid 699) using the above-mentioned outlet end 112 for spitting liquid pipette tips 110
The method that phase property instantaneously accelerates makes to spit the first liquid in liquid pipette tips 110 and is detached from and spits the outlet ends 112 of liquid pipette tips 110 and exist
Microlayer model 199 is formed in oil phase composition (second liquid 699).In scheme one, the first liquid is water phase, oil phase composition (the
Two liquid 699) component be as follows:
Experimental result as shown in FIG. 13 and 14, the volume size homogeneity of the microlayer model 199 in Figure 13 in dotted line frame
Preferably, embodiment 1, embodiment 2, oil phase composition (second liquid 699) shown in embodiment 3 and embodiment 4 are respectively corresponded
Component.As shown in figure 15, when in oil phase composition (second liquid 699)Percentage by volume increase to 15%
And when 16%, the volume size homogeneity of generated microlayer model 199 is poor.It easily obtains, when oil phase composition (second liquid 699)
InPercent by volume be 1.5%-12% when, the volume size homogeneity of generated microlayer model 199 is preferable.
Scheme two: experimental exploring microlayer model 199 generated in the oil phase composition (second liquid 699) of different component
Thermal stability.The generation method of microlayer model 199 is identical as embodiment 1-6.Make the multiple groups oil phase composition containing microlayer model 199
(second liquid 699) undergoes 50 high/low temperature circulations respectively.High/low temperature cyclic process includes: to be risen to the temperature rise rate of 6 DEG C/s
95 DEG C and 10s is maintained, be then down to 65 DEG C with the temperature drop rate of 6 DEG C/s and maintains 10s.Oil used in scheme two is combined
The component of object (second liquid 699) is as follows:
Experimental data as shown in FIG. 16 and 17, the volume size homogeneity of the microlayer model 199 in Figure 16 in dotted line frame
Preferably.When in oil phase composition (second liquid 699)Percent by volume be that 1.5%-12%, P135 containing
When amount is less than 5%, as shown in FIG. 16 and 17, the volume size homogeneity of generated microlayer model 199 is preferable.But when P135's
When content is greater than 5%, as shown in Figure 16 and Figure 18, the volume of generated microlayer model 199 shows feature not of uniform size.Figure 16
The better heat stability of microlayer model 199 in middle solid line.When P135 in oil phase composition (second liquid 699) content between
1.5%-5.0%,Percent by volume when being 1.5%-5.0%, as shown in figure 19, the heat of microlayer model 199 is steady
It is qualitative preferable.When in oil phase composition (second liquid 699)Percent by volume be more than 5.0% when, such as
Shown in Figure 20, the thermal stability of microlayer model 199 is poor.
By the experimental data of 22 embodiments in above-mentioned two scheme it can be concluded that, oil phase composition (second liquid
699) in using mineral oil as main component,Percent by volume be that the content of 1.5%-5.0%, P135 is
When 1.5%-5.0%, the volume size of the first liquid microlayer model 199 generated in oil phase composition (second liquid 699)
Homogeneity is preferably and the thermal stability of microlayer model 199 is also preferable.
Scheme three: experimental exploringPotential substitution ingredient.WithTogether
Belong to the silicon oxygen chain nonionic surface active agent containing chain-like alkyl, the experiment condition of operational version one,Entirely
Portion or part byInstead of.The component of oil phase composition (second liquid 699) is as follows: in scheme three
As shown in figure 21, using the oil phase composition (second of different component shown in embodiment 23 to embodiment 28 in upper table
Liquid 699) the generation experiment of microlayer model 199 is carried out, microlayer model 199 generated all has preferable volume homogeneity.
Scheme four: the potential substitution ingredient of experimental exploring P135.Polyethylene glycol (30) dimerization hydroxy stearic acid ester (P135)
Chain alkane esters are belonged to dimerization hydroxy stearic acid ester (PEG-30) and glycerol stearate.The experiment condition of operational version two,
P135 is replaced by dimerization hydroxy stearic acid ester (PEG-30) and glycerol stearate in whole or in part.Oil phase composition in scheme four
The component of (second liquid 699) is as follows:
As shown in FIG. 22 and 23, using the oil phase composition of different component shown in embodiment 29 to embodiment 32 in upper table
(second liquid 699) carries out the generation of microlayer model 199 and thermal stability experiment, microlayer model 199 generated all have preferable body
Product homogeneity and preferable thermal stability.
During generating microlayer model, the outlet end for spitting liquid pipette tips is kept in motion, the flow velocity for being discharged liquid is unstable,
It is uncontrollable.Randomness is presented in microlayer model volume size generated.
Based on this, it is necessary to for spit liquid pipette tips during exercise because discharge liquid flow velocity it is unstable, it is uncontrollable caused by
The problem of microlayer model volume size shows randomness, providing one kind can guarantee to spit liquid pipette tips according to setting flow velocity discharge liquid
Fluid driving mechanism.
In the generating process of microlayer model 199, the first liquid spits the outlet end of liquid pipette tips 110 with the flow velocity discharge set
112.Spit liquid pipette tips 110 outlet end 112 do comprising instantaneously accelerate periodic motion when, microlayer model can not only be effectively generated
199, and convenient for controlling the size of generated microlayer model 199.Displacement is done in sine in the outlet end 112 for spitting liquid pipette tips 110
When the periodic motion of variation, microlayer model 199 can not be effectively generated, and generated microlayer model 199 has good volume
Size homogeneity.During above two generation microlayer model 199, the first liquid is under the driving of fluid driving mechanism 120
The outlet end 112 of liquid pipette tips 110 is spat with the flow velocity discharge of setting.
As shown in Figure 24 and Figure 25, the present invention provides a kind of fluid driving mechanism 120, is used in microlayer model generation system,
Including capacity component 121 and Power Component 122.Capacity component 121 includes injection tube 1211 and push rod 1212.Push rod 1212
It is slidably matched with the inner wall of injection tube 1211, driving liquid 1214 can be stored in injection tube 1211.Injection tube 1211 have into
Liquid outlet 1213, liquid in-out mouth 1213 is for being connected to the arrival end for spitting liquid pipette tips 110 for storing the first liquid 190.Power packages
Part 122 and push rod 1212 are sequentially connected, and the extending direction for driving push rod 1212 along injection tube 1211 slides.In microlayer model
In 199 generating process, the extruding of 122 driving push rod 1212 of Power Component, which is stored in injection tube 1211, drives liquid 1214, drives
The extruding of hydrodynamic body 1214 is stored in the first liquid 190 spat in liquid pipette tips 110, and then by the first liquid 190 from spitting liquid pipette tips 110
Outlet end 112 be discharged.Fluid driving mechanism 120 provided by the invention utilizes the incompressible of liquid (driving liquid 1214)
Property ensure that spit the outlet ends 112 of liquid pipette tips 110 remained in high-frequency vibration flow velocity according to setting by the first liquid 190 from
The outlet end 112 for spitting liquid pipette tips 110 is discharged.Fluid driving mechanism 120 provided by the invention can accurately control generated micro- liquid
Drip 199 volume sizes.Fluid driving mechanism 120 provided by the present invention is not limited to the above embodiment, for example, can also adopt
With peristaltic pump, pressure-driven pump, pneumatic-driven pump or driven by electroosmosis pump etc..
As a kind of achievable mode, the liquid in-out mouth 1213 of injection tube 1211 with spit liquid pipette tips 110 arrival end it
Between be connected to by tubule 123.Driving liquid 1214 is stored in injection tube 1211 and in tubule 123.Power Component 122 and transfiguration
The push rod 1212 of product component 121 is sequentially connected, and Power Component 122 is for pushing the push rod 1212 of capacity component 121 injecting
Sliding in cylinder 1211.In the generating process of microlayer model 199, Power Component 122 pushes the push rod 1212 of capacity component 121, pushes away
Bar 1212 squeezes the driving liquid 1214 being stored in injection tube 1211 and tubule 123, and the driving extruding of liquid 1214, which is stored in, spits
The first liquid 190 in liquid pipette tips 110, and then the first liquid 190 is discharged from the outlet end 112 for spitting liquid pipette tips 110.Using thin
The liquid in-out mouth 1213 of injection tube 1211 is connect by pipe 123 with the arrival end for spitting liquid pipette tips 110, the internal diameter of one side tubule 123
It is smaller, convenient for realizing the accurate control to discharge liquid volume by the stroke of control push rod 1212；On the other hand, using tubule
123 flexible arrangement injection tube 1211 and can spit the position between liquid pipette tips 110 and distance, convenient in injection tube 1211 and spitting liquid
Other necessaries are arranged between pipette tips 110.
In an embodiment of the present invention, Power Component 122 pushes push rod 1212 at the uniform velocity to slide in injection tube 1211, implies that
Liquid 1214 is driven to be discharged with uniform flow velocity from the liquid in-out mouth 1213 of capacity component 121 under the promotion of push rod 1212,
Entered by tubule 123 with uniform flow velocity and spits liquid pipette tips 110.The first liquid 190 spat in liquid pipette tips 110 is stored in drive
Under the promotion of liquid 1214, the outlet end 112 of liquid pipette tips 110 is spat with the discharge of uniform flow velocity.By using driving liquid 1214
It does driving medium and control push rod 1212 and driving liquid 1214, fluid driving provided in this embodiment is discharged with uniform flow velocity
Mechanism 120 can not only when spitting liquid pipette tips 110 and remaining static with uniform flow velocity by the first liquid 190 by spitting liquid pipette tips
110 outlet end 112 is discharged.Even if spitting liquid pipette tips 110 to be under fast vibration state, fluid driver provided in this embodiment
Structure 120 still ensures that the first liquid 190 from the outlet end 112 for spitting liquid pipette tips 110 with the discharge of uniform flow velocity.The present embodiment provides
Fluid driving mechanism 120 substantially increase the volume size homogeneity of generated microlayer model 199.
The effect of Power Component 122 is to drive push rod 1212 in injection tube 1211 along the direction far from liquid in-out mouth 1213
Or it is slided close to the direction of liquid in-out mouth 1213.Optionally, Power Component 122 can be cylinder, hydraulic cylinder etc. and directly export
The component of linear motion is also possible to convert circular motion to the component of linear motion, as motor and synchronous pulley combination,
Motor and screw rod 1222 and the combination of sliding block 1223 etc..The present invention is not intended to limit the specific structure of Power Component 122.Such as Figure 25 institute
Show, in an embodiment of the present invention, Power Component 122 includes driving motor 1221, screw rod 1222 and sliding block 1223.Driving motor
1221 output shaft and one end of screw rod 1222 are sequentially connected, and sliding block 1223 has internal screw thread, sliding block 1223 and 1222 table of screw rod
The external screw thread in face is cooperatively connected.The outer rim of sliding block 1223 is fixedly connected with push rod 1212 far from one end of injection tube 1211.Sliding block
1223 convert sliding block 1223 along 1222 axial direction of screw rod for the rotary motion that driving motor 1221 exports with the cooperation of screw rod 1222
Linear motion, so that the push rod 1212 of capacity component 121 be driven to slide in injection tube 1211.Further, in the present embodiment
The driving motor 1221 used is servo motor.Servo motor has the characteristics that accurate feedback and control angle displacement.
As shown in figure 26, in an embodiment of the present invention, fluid driving mechanism 120 further includes three-way diverter valve 124 and storage
Flow container 125.Three-way diverter valve 124 has first interface, second interface and third interface.Spit arrival end, the transfiguration of liquid pipette tips 110
Product component 121 liquid in-out mouth 1213 and fluid reservoir 125 respectively with the first interface of three-way diverter valve 124, second interface and
Third orifice.Three-way diverter valve 124 at least can control fluid driving mechanism 120 and realize following two mode: one, making to become
The liquid in-out mouth 1213 of volume component 121 is connected with the arrival end for spitting liquid pipette tips 110, under the drive of Power Component 122, becomes
Volume component 121 provides liquid driven power to liquid pipette tips 110 are spat, for that will spit the first liquid 190 in liquid pipette tips 110 from spitting liquid
The outlet end 112 of pipette tips 110 is discharged, or the first liquid 190 is drawn into from the outlet end 112 for spitting liquid pipette tips 110 and spits liquid
In pipette tips 110.Two, the liquid in-out mouth 1213 of capacity component 121 is made to be connected with fluid reservoir 125, in the band of Power Component 122
Under dynamic, the driving liquid 1214 in fluid reservoir 125 is drawn into the injection tube of capacity component 121 by capacity component 121
In 1211, or will be in the driving liquid push-in fluid reservoir 125 in capacity component 121.
As shown in figure 26, one embodiment of the invention also provides a kind of fluid driving method, using above-mentioned fluid driving mechanism,
The following steps are included: (1) three-way diverter valve 124 is connected to the liquid in-out mouth 1213 of capacity component 121 with fluid reservoir 125.?
Under the drive of Power Component 122, push rod 1212 changes note to one end sliding far from liquid in-out mouth 1213 in injection tube 1211
The volume of shooting cylinder 1211 sucks the driving liquid 1214 in fluid reservoir 125 in injection tube 1211.(2) three-way diverter valve 124
It is connected to the liquid in-out mouth 1213 of capacity component 121 with the arrival end for spitting liquid pipette tips 110.Under the drive of Power Component 122,
Push rod 1212 slides the volume for changing injection tube 1211 in injection tube 1211 to one end close to liquid in-out mouth 1213, with discharge
In injection tube 1211, in tubule 123 and spit gas in liquid pipette tips 110.(3) outlet end 112 for spitting liquid pipette tips 110 is entered
In first liquid 190, and three-way diverter valve 124 is maintained to make the liquid in-out mouth 1213 of capacity component 121 and spit liquid pipette tips 110
Arrival end connection.Under the drive of Power Component 122, push rod 1212 is in injection tube 1211 to one far from liquid in-out mouth 1213
End sliding changes the volume of injection tube 1211, and 190 inspiration of the first liquid is spat in liquid pipette tips 110.(4) three-way diverter valve is maintained
124 are connected to the liquid in-out mouth 1213 of capacity component 121 with the arrival end for spitting liquid pipette tips 110.In the drive of Power Component 122
Under, push rod 1212 at the uniform velocity slides the appearance for changing injection tube 1211 in injection tube 1211 to one end close to liquid in-out mouth 1213
Product will be stored in and spit the outlet end that the first liquid 190 in liquid pipette tips 110 spits liquid pipette tips 110 with the discharge of uniform flow velocity
112。
For the ease of smoothly the gas in injection tube 1211 is discharged in above-mentioned second step, as shown in figure 25, peace
Upward, push rod 1212 slides in injection tube 1211 the liquid in-out mouth 1213 of injection tube 1211 along the vertical direction when dress.
In order to improve the formation efficiency of microlayer model 199, as a kind of achievable mode, the quantity for spitting liquid pipette tips 110 is
It is multiple, it is multiple to spit the setting of 110 spacing side by side of liquid pipette tips or arranged in the form of other.Each spitting liquid pipette tips 110 passes through list
Only tubule 123 is connected to the first interface of three-way diverter valve 124.The quantity of capacity component 121 is one, capacity component
121 liquid in-out mouth 1213 is connected to the second interface of three-way diverter valve 124.The third interface and liquid storage of three-way diverter valve 124
Tank 125 is connected to.Under the driving of Power Component 122, push rod 1212 is in injection tube 1211 along the side close to liquid in-out mouth 1213
To at the uniform velocity sliding, while driving liquid 1214 being squeezed to multiple and is spat in liquid pipette tips 110.Due to being simultaneously between multiple tubules 123
The flow of connection relationship, the driving liquid 1214 in each tubule 123 is identical, ensure that multiple the first liquid spat in liquid pipette tips 110
Body 190 spits the outlet end 112 of liquid pipette tips 110 with identical, constant flow velocity discharge.And then it ensure that generated microlayer model 199
Volume size homogeneity.
In order to improve the formation efficiency of microlayer model 199, as another achievable mode, liquid pipette tips 110 and transfiguration are spat
The quantity of product component 121 is multiple.It is multiple to spit the setting of 110 spacing side by side of liquid pipette tips or arranged in the form of other.Each
Liquid pipette tips 110 are spat to be connected to by individual tubule 123 with the first interface of three-way diverter valve 124.Each capacity component 121
Liquid in-out mouth 1213 be connected to also by individual tubule 123 with the second interface of three-way diverter valve 124.Three-way diverter valve 124
Third interface be connected to fluid reservoir 125.Multiple 121 spacing side by side of capacity component settings are arranged in the form of other.
The push rod 1212 of multiple capacity components 121 is relatively fixed far from one end of injection tube 1211, is synchronized and is pushed away by Power Component 122
It is dynamic.Under the driving of Power Component 122, multiple push rods 1212 are in respective injection tube 1211 along close to liquid in-out mouth 1213
Direction is at the uniform velocity slided, while driving liquid 1214 being squeezed to multiple and is spat in liquid pipette tips 110.Due to being between multiple tubules 123
The flow of parallel relationship, the driving liquid 1214 in each tubule 123 is identical, ensure that multiple first spat in liquid pipette tips 110
Liquid 190 spits the outlet end 112 of liquid pipette tips 110 with identical, constant flow velocity discharge.And then it ensure that generated microlayer model 199
Volume size homogeneity.
In order to improve the formation efficiency of microlayer model 199, as the third achievable mode, as shown in figure 27, liquid rifle is spat
First 110, the quantity of capacity component 121 and three-way diverter valve 124 is identical and is multiple.Each spit the entrance of liquid pipette tips 110
End is connected to the first interface of a three-way diverter valve 124 respectively by individual tubule 123.Each capacity component 121
Liquid in-out mouth 1213 is connected to the second interface of a three-way diverter valve 124 respectively by individual tubule 123.Each threeway is changed
It is connected to respectively with fluid reservoir 125 to the third interface of valve 124.Optionally, fluid reservoir 125 can be one or more.Each
The first liquid 190 spat in liquid pipette tips 110 can be the same or different.The setting of multiple 121 spacing side by side of capacity component or
Person is arranged in the form of other.The push rod 1212 of multiple capacity components 121 is relatively fixed far from one end of injection tube 1211, by
Power Component 122 is synchronous to be pushed.Under the driving of Power Component 122, multiple push rods 1212 edge in respective injection tube 1211
It at the uniform velocity slides in direction close to liquid in-out mouth 1213.A variety of different types of microlayer models 199 can be generated simultaneously.
In order to improve the formation efficiency of microlayer model 199, as the 4th kind of achievable mode, liquid pipette tips 110, capacity are spat
The quantity of component 121 and three-way diverter valve 124 is identical and is multiple.The arrival end for each spitting liquid pipette tips 110 passes through individually
Tubule 123 is connected to the first interface of a three-way diverter valve 124 respectively.The liquid in-out mouth 1213 of each capacity component 121
It is connected to respectively with the second interface of a three-way diverter valve 124 by individual tubule 123.The of each three-way diverter valve 124
Three interfaces are connected to fluid reservoir 125 respectively.Optionally, fluid reservoir 125 can be one or more.Each spit liquid pipette tips 110
The first interior liquid 190 can be the same or different.Multiple 121 spacing side by side of capacity component are arranged or with other shapes
Formula arrangement.Each capacity component 121 respectively corresponds individual Power Component 122.It is multiple under the driving of Power Component 122
Push rod 1212 at the uniform velocity slides in respective injection tube 1211 along the direction close to liquid in-out mouth 1213.It can not only generate simultaneously
A variety of different types of microlayer models 199 are guaranteeing that the volume for each spitting the generated microlayer model 199 of liquid pipette tips 110 is uniform
Under in advance, additionally it is possible to control the volume size of every kind of drop 195 respectively.Convenient for raw to multiple microlayer models 199 for spitting liquid pipette tips 110
It is independently controlled at state.
Microlayer model generating means and generation method provided by the invention are in clinical medicine inspection, nano material preparation, food
And the application fields such as environment measuring, biochemical analysis are all widely used.It is provided by the invention in a specific application environment
The generating means and generation method of microlayer model 199 apply polymerase chain reaction (Polymerase Chain Reaction,
PCR in).
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (12)
1. a kind of microlayer model, which generates, uses oil phase composition, which is characterized in that including following components:
Mineral oil, the percent by volume of mineral oil described in the oil phase composition are 88%-98.5%；
Surfactant, the surfactant include the silicon oxygen chain nonionic surface active agent containing chain-like alkyl.
2. microlayer model according to claim 1, which generates, uses oil phase composition, which is characterized in that institute in the oil phase composition
The percent by volume for stating the silicon oxygen chain nonionic surface active agent containing chain-like alkyl is 1.5%-12%.
3. microlayer model according to claim 1, which generates, uses oil phase composition, which is characterized in that the silicon containing chain-like alkyl
Oxygen chain nonionic surface active agent includesEM90 andOne or both of EM180.
4. microlayer model according to claim 3, which generates, uses oil phase composition, which is characterized in that the surfactant also wraps
Chain alkane esters are included, chain alkane esters described in the oil phase composition and the mass volume ratio of the oil phase composition are
0.015g/mL-0.05g/mL。
5. microlayer model according to claim 4, which generates, uses oil phase composition, which is characterized in that the chain alkane esters include
One in dimerization hydroxy stearic acid ester (PEG-30), glycerol stearate and polyethylene glycol (30) dimerization hydroxy stearic acid ester (P135)
Kind is a variety of.
6. microlayer model according to claim 5, which generates, uses oil phase composition, which is characterized in that the chain alkane esters are poly-
Ethylene glycol (30) dimerization hydroxy stearic acid ester (P135).
7. microlayer model according to claim 5, which generates, uses oil phase composition, which is characterized in that contain in the oil phase composition
The percent by volume of the silicon oxygen chain nonionic surface active agent of chain-like alkyl is 1.5%-5.0%.
8. microlayer model according to claim 1-7, which generates, uses oil phase composition, which is characterized in that described to contain chain
The silicon oxygen chain nonionic surface active agent of alkyl isEM90。
9. a kind of processing method of oil phase composition, which is characterized in that for handling the described in any item oily phases of claim 1-7
Composition；The processing method of the oil phase composition includes heating the oil phase composition, while the oil phase composition being set
In the environment of negative pressure and ultrasonic activation.
10. the processing method of oil phase composition according to claim 9, which is characterized in that further include making the oily phase group
It closes object and reaches water saturated step.
11. the processing method of oil phase composition according to claim 10, which is characterized in that described to be combined the oil
It includes: that distilled water is added in the oil phase composition before heating the oil phase composition that object, which reaches water saturated step,；With
And after the heating process of the oil phase composition, oil phase composition natural cooling under 25 DEG C of -35 DEG C of environment.
12. the processing method of oil phase composition according to claim 11, which is characterized in that after nitrogen protection cooling
The oil phase composition.
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Cited By (1)
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| WO2021037218A1 (en) * | 2019-08-30 | 2021-03-04 | 北京达微生物科技有限公司 | Sample adding needle for preparing microdroplets and microdroplet preparation method |
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Application publication date: 20190730 |