CN208092059U - A kind of upper forwarding light immune chromatography test paper carrying out quantitative detection to crystal methamphetamine concentration in saliva - Google Patents
A kind of upper forwarding light immune chromatography test paper carrying out quantitative detection to crystal methamphetamine concentration in saliva Download PDFInfo
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- CN208092059U CN208092059U CN201720877301.1U CN201720877301U CN208092059U CN 208092059 U CN208092059 U CN 208092059U CN 201720877301 U CN201720877301 U CN 201720877301U CN 208092059 U CN208092059 U CN 208092059U
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Abstract
A kind of upper forwarding light immune chromatography test paper carrying out quantitative detection to crystal methamphetamine concentration in saliva, including sample pad [1], bonding pad [2], analyzing film [3], water absorption pad [4], sticky bottom liner [5] and Max lines indicating sticker [6], the sample pad [1] is double-layer structure, upper layer is the glass fibre membrane [11] of netted capillary structure, lower layer is the filter saliva layer [12] that hemofiltration pad is formed after reagent is handled, glass fibre membrane [11] is with overlay film, the mode of suture or bonding is fitted in filter saliva layer [12] upper surface.Water absorption pad forms the first combined area with analyzing film, and bonding pad forms the second combined area with analyzing film, and sample pad forms third combined area with bonding pad, respectively combines section length and slightly short length is the 3/10-2/5 of respective pads body length.It can be applied using the utility model and Quantitative detection is carried out to the crystal methamphetamine in saliva at the scene, convenient, efficient detection means is provided for drugs screening.
Description
Technical field
The utility model is related to a kind of test paper structures based on upper forwarding light immunochromatography technique, more particularly to one kind can be right
Crystal methamphetamine concentration carries out the test paper based on upper forwarding light immunochromatography technique of accurate quantification detection in saliva.
Background technology
Crystal methamphetamine (methamphetamine, MA) is commonly called as ice.There is methamphetamine strong central nervous excitation to make
With strong toxicity is one of the most important drugs for being monitored, banning.
Traditional illicit drugs inspection is urine detection, since the sampling of its sample is inconvenient, awkward, must have special taking to urinate place；
In addition urine sampling is easy adulterated, switch, and palpus one-to-one exercise supervision of someone takes urine；Such waste of manpower, material resources, cause manpower
It is insufficient.It takes in particular for women suspect and need to be supervised by women staff when urine sample, and women staff's critical shortage.
Due to disadvantages mentioned above, urine specimen is gradually replaced by saliva sample, becomes the mainstream of illegal drug screening sample.
The upper forwarding light immunochromatography technique (Up- detected for crystal methamphetamine in urine existing at present
Converting Phosphor Technology based Lateral Flow assay, UPT-LF assay), by urine
Sample be added it is special by sample pad, bonding pad, analyzing film, water absorption pad, sticky bottom liner and Max line indicating stickers form upper turn
Electrochemiluminescent immunoassay chromatographic test paper (ZL201320550667X) generates the immune chromatography test paper using upper forwarding light immunity analysis instrument
Nonspecific signal be detected, to in urine crystal methamphetamine concentration carry out accurate quantification.However, due to saliva sample and
In urine sample there is larger difference in ingredient and dispersibility, existing suitable for turning electrochemiluminescent immunoassay layer in urine sample detection
The detection of saliva sample can not be suitable for by analysing (UPT-LF) test paper, it is necessary to which exploitation is quantitatively examined for crystal methamphetamine in saliva
The upper forwarding light immune chromatography test paper surveyed.
Invention content
In order to overcome the deficiencies in the prior art, the utility model aim to be to provide a kind of to methylbenzene in saliva
Propylamine concentration carries out upper forwarding light immunochromatography (UPT-LF) test paper of quantitative detection.
It is provided by the utility model it is a kind of to crystal methamphetamine concentration in saliva carry out quantitative detection on turn electrochemiluminescent immunoassay
(UPT-LF) test paper is chromatographed, including：Including sample pad, bonding pad, analyzing film, water absorption pad, sticky bottom liner and Max line indicating stickers,
The wherein described sample pad be double-layer structure, upper layer be netted capillary structure glass fibre membrane, lower layer be hemofiltration pad through reagent at
The filter saliva layer formed after reason, glass fibre membrane are fitted in filter saliva layer upper surface in a manner of overlay film, suture or bonding；It is described
Water absorption pad be affixed on the side of analyzing film and with analyzing film formed the first combined area, bonding pad be affixed on the other side of analyzing film and with point
It analyses film and forms the second combined area, sample pad is affixed on the other side of bonding pad and forms third combined area, Max lines with bonding pad
Indicating sticker is pasted in bonding pad and sample pad and covering bonding pad；Described first combines section length for the 3/ of water absorption pad length
, described second combines section length for the 3/10-2/5 of bonding pad length；The third combination section length is that sample pad is long
The 3/10-2/5 of degree.
Wherein, filter saliva layer is the hemofiltration pad of buffered liquid, surfactant, closed protein, inorganic salt treatment.
The edge of Max lines indicating sticker side Chong Die with bonding pad is concordant with bonding pad, the edge of side Chong Die with sample pad
Slightly shorter than print pad, slightly short partial-length is the 3/10-2/5 of sample pad length.
Specifically, described first combines section length for the 7/20 of water absorption pad length；Described second combines section length to combine
Pad the 7/20 of length；The third combination section length is the 7/20 of sample pad length；
Parallel detection band T and quality control band C is provided on analyzing film in length direction spacing, first is coated in detection band T
Base amphetamine-bovine serum albumin(BSA) crosslinking antigen is coated with rabbit-anti sheep IgG in quality control band C.
UCP conjugate mixtures are fixed in bonding pad, UCP conjugate mixtures include：Detect conjugate and Quality Control
Conjugate, detection conjugate are UCP- crystal methamphetamine monoclonal antibody conjugates, and Quality Control conjugate is that UCP- sheep IgG is combined
Object.
Using above scheme, the upper forwarding light that the utility model carries out crystal methamphetamine concentration in saliva quantitative detection is exempted from
Epidemic disease chromatographs (UPT-LF) test paper, first against the character of saliva sample, sample pad is designed as double-layer structure, upper layer is netted hair
Thin glass fiber film makes saliva sample rapid dispersion, lower layer be special buffered liquid, surfactant, closed protein, nothing first
The filter saliva layer of machine salt treatment, can reduce the interference of each ingredient in saliva to greatest extent；In addition the overlapping that entire test paper is related to
Region includes " water absorption pad first combined area Chong Die with analyzing film ", " bonding pad second combined area Chong Die with analyzing film ", " sample
The product pad third combined area Chong Die with bonding pad " is all made of the partially slight overlap mode of moderate, according to identified detection object
The combination of crystal methamphetamine and reagent and its features of movement on test paper, design overlap margin be respective 3/10-2/5 (i.e.
), to take into account adequacy that liquid sample is laterally therein flowed in test paper each section simultaneously and liquid sample exists
Sample pad and the abundant infiltration mixing in bonding pad, on the one hand ensure more suitable flowing time, on the other hand so that sample
In detection target and detection conjugate Chong Die fully reacted by the appropriate of sample pad and bonding pad.
Therefore, the upper forwarding light that quantitative detection is carried out to crystal methamphetamine concentration in saliva that the utility model is established is exempted from
Epidemic disease chromatographs (UPT-LF) test paper, remains that easy to operate, quick, sensibility is high, specificity is good, can many spies such as accurate quantification
Property, this completely new field can be more detected for saliva sample and proposes feasible solution, can be applied at the scene to saliva
In crystal methamphetamine carry out Quantitative detection, overcome to urine sample detect caused by it is inconvenient.
Description of the drawings：
Fig. 1：The utility model is to crystal methamphetamine quantitative testing test paper three-dimensional structure diagram in saliva；
Fig. 2：The utility model is to crystal methamphetamine quantitative testing test paper cross-sectional view in saliva；
Fig. 3：The utility model pastes schematic diagram to crystal methamphetamine quantitative testing test paper in saliva；
Fig. 4：The standard curve that crystal methamphetamine standard concentration in saliva is detected.
1, sample pad, 11, glass fibre membrane, 12, filter saliva layer, 2, bonding pad, 3, analyzing film, 4, water absorption pad, 5, viscosity
Bottom liner, 6, Max line indicating stickers, 7, UCP conjugate mixtures, 8, UCP- crystal methamphetamine monoclonal antibody conjugates, 9, UCP-
Sheep IgG conjugates, 10, detection band T, 11, quality control band C.
The utility model is further illustrated below in conjunction with the accompanying drawings.
Specific implementation mode：
Shown in referring to Fig. 1 and Fig. 2, the utility model is provided carries out the upper of quantitative detection to crystal methamphetamine concentration in saliva
Light immunochromatography (UPT-LF) test paper is forwarded, entirety is by wide ribbon sample pad 1, bonding pad 2, analyzing film 3, water absorption pad
4, sticky bottom liner 5, more than 6 part of Max lines indicating sticker and test paper shell composition.Wherein：
Sample pad 1 is double-layer structure, and upper layer is glass fibre membrane 11, is netted capillary fiber structure, can adsorb than same
The more moisture of equal cellulosic filter papers, make touched saliva sample rapid dispersion；Lower layer is filter saliva layer 12, usually fine
The hemofiltration pad of thickness 1-2cm made of the plain paper material of dimension, through suitable buffer solution, surfactant, closed protein, inorganic salts
Processing, can reduce the interference of each ingredient in saliva to greatest extent；Glass fibre membrane 11 can be fitted in filter saliva in a manner of overlay film
12 upper surface of liquid layer, is pressed by test paper shell, or is sutured at edge according to designed test paper size, or using without containing interference
Crystal methamphetamine detects the glue bond of ingredient.
UCP conjugates mixture 7 is fixed in bonding pad 2, UCP conjugates mixture 7 includes：Detect conjugate and matter
Conjugate is controlled, detection conjugate is UCP- crystal methamphetamine monoclonal antibodies conjugate 8, and Quality Control conjugate is UCP- sheep IgG knots
Close object 9；
Detection band T10, quality control band C11 are provided on analyzing film 3, two bands are mutually parallel and have certain in the longitudinal direction
Interval；Detection band T10 is coated with crystal methamphetamine-bovine serum albumin(BSA) crosslinking antigen, and quality control band C11 is coated with rabbit-anti sheep IgG；
Sample pad 1, bonding pad 2, analyzing film 3 and water absorption pad 4 are pasted on successively on sticky bottom liner 5, and wherein analyzing film 3 is affixed on
The centre of sticky bottom liner 5, water absorption pad 4 are affixed on the side of analyzing film 3, the first combined area of the two intersection formation, and first
In conjunction with section length L1 be 4 length of water absorption pad 3/10-2/5, preferably 7/20；Bonding pad 2 be affixed on analyzing film 3 the other side it
On, the two intersection forms the second combined area, and second combines section length L2 for the 3/10-2/5 of 2 length of bonding pad, preferably
7/20；Sample pad 1 is affixed on the other side of bonding pad 2, and the two intersection forms third combined area, the third combination head of district
Spend L3 be 1 length of sample pad 3/10-2/5, preferably 7/20；Max lines indicating sticker 6 is pasted on bonding pad 2 and sample pad 1
And covering bonding pad 2, wherein side Chong Die with bonding pad 2, edge are concordant with bonding pad 2；Side Chong Die with sample pad 1, edge
It is slightly shorter than print pad 1, slightly short partial-length L4 be 1 length of sample pad 3/10-2/5, preferably 7/20.
Preparation method of forwarding light immunochromatography (UPT-LF) test paper is on this：
A. the preparation of sample pad 1：By 4ml sample pads treatment fluid (pH7.2 phosphate buffers, containing 0.01%SDS,
0.5% skimmed milk power, 2%NaCl) it is added drop-wise on the hemofiltration pad of 20cm × 1.5cm, 2h is dried at 37 DEG C, obtains lower layer's filter saliva
Layer 12, upper layer cover one layer of simple glass tunica fibrosa 11, and coverage mode is overlay film, suture or bonding, obtains sample pad 1, spare；
B. the preparation of bonding pad 2：It will detection conjugate (UCP- crystal methamphetamine monoclonal antibodies conjugate 8) and Quality Control
Conjugate (UCP- sheep IgG conjugates 9) mixes, and obtains UCP conjugates mixture 7, and with conjugate dilution, (pH7.2 phosphate is slow
Fliud flushing, contains 1% skimmed milk power) UCP conjugates mixture 7 is diluted to final concentration of 2mg/ml, by UCP conjugates mixture 7
It is added on glass fibre, polyester film or non-woven fabrics, 1.5h is dried at 45 DEG C, obtain bonding pad 2, it is spare；
C. the preparation of analyzing film 3：By 2mg/ml crystal methamphetamines-bovine serum albumin(BSA) crosslinking antigen and 2mg/ml rabbit-antis
Sheep IgG speckings on nitrocellulose filter or nylon membrane respectively as detection band T10 and quality control band C11, dry 1h at 37 DEG C,
Analyzing film 3 is obtained, it is spare；
D. sample pad 1, bonding pad 2, analyzing film 3 and water absorption pad 4 are pasted on sticky bottom liner 5 successively and (PVC board etc. may be selected
Water proof material) on, Max lines indicating sticker 6 water proofs materials such as (may be selected) PVC films is pasted on sample pad 1, on bonding pad 2, it is ensured that
Mutual overlapping relation (as shown in Figure 3)；Test paper is cut into 4mm wide individually available finished products, obtains the utility model saliva
Crystal methamphetamine concentration quantitative Test paper in liquid；Molding test paper can be used directly or be placed in plastic shell and uses.
It is as follows that quantitative detection process is carried out to crystal methamphetamine concentration in saliva using the utility model：
A. sample is added：Sample to be tested (saliva) and sample treatment liquid (pH7.2 phosphate buffers) are pressed 1:9 ratios
After mixing, 100 μ l/ items are added dropwise on the glass fibre 11 of above-mentioned sample pad 1；
B. chromatography reaction：It stands several minutes and waits for that chromatography reaction is completed；
C. result interpretation：Test paper is scanned with commercially available drug abuse in-situs tester, by detection band T [10] and quality control band C
[11] for assignment in T and C, T/C is the detected value of crystal methamphetamine to signal strength (i.e. peak area) successively；
D. result is quantitative：It is corresponding with each concentration to be measured acquisition for p-Methylamphetamine series concentration standard items successively
T/C values draw normal concentration working curve and fitting quantitative calculation formula using T/C values as X, using standard concentration as Y,
The T/C values that each sample detection obtains are substituted into quantitative calculation formula (Quantitative equation is shown in Fig. 4) and can be obtained methyl contained therein
The Exact concentrations of amphetamine.
Upper forwarding light immunochromatography (UPT-LF) the test paper detection of quantitative detection is carried out to crystal methamphetamine concentration in saliva
Crystal methamphetamine standard items sensibility and the range of linearity：
After the common saliva dilution of the crystal methamphetamine standard items purchased from Material Evidence Identification Center, Ministry of Public Security, with sample treatment
Liquid (pH7.2 phosphate buffers) presses 1:9 ratio mixings, 100 μ l/ items are added to the utility model crystal methamphetamine and quantitatively detect examination
On paper, scanned after chromatographing several minutes, standard curve is shown in Fig. 4, the experimental result obtained such as table 1：
Table 1：The utility model crystal methamphetamine quantitative testing test paper detects crystal methamphetamine standard items sensibility
As it can be seen that being detected to the crystal methamphetamine in saliva using the utility model crystal methamphetamine quantitative testing test paper sensitive
Property is 25ng/ml, can be with accurate quantification in the range of 25-10000ng/ml.The utility model can be efficiently applied to saliva
The quantitative detection of trace methyl amphetamine in sample provides convenient, efficient detection means for drugs screening.
Claims (5)
1. a kind of upper forwarding light immune chromatography test paper carrying out quantitative detection to crystal methamphetamine concentration in saliva, including sample pad
[1], bonding pad [2], analyzing film [3], water absorption pad [4], sticky bottom liner [5] and Max lines indicating sticker [6], the sample pad [1],
Bonding pad [2], analyzing film [3] and water absorption pad [4] are pasted on successively on sticky bottom liner [5], and wherein analyzing film [3] is affixed on sticky bottom
The centre of [5] is served as a contrast, water absorption pad [4] is affixed on the side of analyzing film [3] and forms the first combined area, bonding pad [2] with analyzing film [3]
It is affixed on the other side of analyzing film [3] and forms the second combined area with analyzing film [3], sample pad [1] is affixed on the another of bonding pad [2]
Third combined area is formed on side and with bonding pad [2], Max lines indicating sticker [6] is pasted in bonding pad [2] and sample pad [1]
And covering bonding pad [2]；It is characterized in that：
The sample pad [1] is double-layer structure, and upper layer is the glass fibre membrane [11] of netted capillary structure, and lower layer passes through for hemofiltration pad
The filter saliva layer [12] formed after reagent processing, glass fibre membrane [11] are fitted in filter saliva in a manner of overlay film, suture or bonding
Liquid layer [12] upper surface；
Described first combines section length for the 3/10-2/5 of water absorption pad [4] length, and described second combines section length for bonding pad [2]
The 3/10-2/5 of length；The third combination section length is the 3/10-2/5 of sample pad [1] length；
Length direction spacing is provided with parallel detection band T [10] and quality control band C [11], detection band T on the analyzing film [3]
[10] it is coated with crystal methamphetamine-bovine serum albumin(BSA) crosslinking antigen in, rabbit-anti sheep IgG is coated in quality control band C [11]；
UCP conjugates mixture [7] is fixed in the bonding pad [2], UCP conjugates mixture [7] includes：Detection combines
Object and Quality Control conjugate, detection conjugate are UCP- crystal methamphetamine monoclonal antibody conjugates [8], and Quality Control conjugate is UCP-
Sheep IgG conjugates [9].
2. upper forwarding light immune chromatography test paper as described in claim 1, which is characterized in that Max lines indicating sticker [6] and bonding pad
[2] edge of overlapping side is concordant with bonding pad [2], and the edge of side Chong Die with sample pad [1] is slightly shorter than print pad [1], slightly
Short partial-length is the 3/10-2/5 of sample pad [1] length.
3. upper forwarding light immune chromatography test paper as claimed in claim 1 or 2, which is characterized in that described first combines section length
It is the 7/20 of water absorption pad [4] length；Described second combines section length for the 7/20 of bonding pad [2] length；The third combined area
Length is the 7/20 of sample pad [1] length.
4. as claimed in claim 3 upper forward light immune chromatography test paper, which is characterized in that the Max lines indicating sticker [6] by every
Water material is made.
5. upper forwarding light immune chromatography test paper as claimed in claim 4, which is characterized in that filter saliva layer [12] is buffered
The hemofiltration pad of liquid, surfactant, closed protein, inorganic salt treatment.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720877301.1U CN208092059U (en) | 2017-07-19 | 2017-07-19 | A kind of upper forwarding light immune chromatography test paper carrying out quantitative detection to crystal methamphetamine concentration in saliva |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720877301.1U CN208092059U (en) | 2017-07-19 | 2017-07-19 | A kind of upper forwarding light immune chromatography test paper carrying out quantitative detection to crystal methamphetamine concentration in saliva |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116577498A (en) * | 2023-07-13 | 2023-08-11 | 济南玖方生物科技有限公司 | Application of sample pad for detecting HIV antibody in urine, test strip and sample pad treatment fluid |
-
2017
- 2017-07-19 CN CN201720877301.1U patent/CN208092059U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116577498A (en) * | 2023-07-13 | 2023-08-11 | 济南玖方生物科技有限公司 | Application of sample pad for detecting HIV antibody in urine, test strip and sample pad treatment fluid |
| CN116577498B (en) * | 2023-07-13 | 2023-09-12 | 济南玖方生物科技有限公司 | Application of sample pad for detecting HIV antibody in urine, test strip and sample pad treatment fluid |
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Effective date of registration: 20210114 Address after: 100027 room 20b07, C 2, East Third Ring Road North, Chaoyang District, Beijing Patentee after: CHINA INTELLECTECH BEIJING Corp. Address before: 100029 No.18, Rome Garden, 18 Huixin West Street, Chaoyang District, Beijing Patentee before: Yang Xiaoman |
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| TR01 | Transfer of patent right |