CN208055327U - A kind of hot lid mechanism of fluorescence quantitative PCR instrument - Google Patents
A kind of hot lid mechanism of fluorescence quantitative PCR instrument Download PDFInfo
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- CN208055327U CN208055327U CN201820377542.4U CN201820377542U CN208055327U CN 208055327 U CN208055327 U CN 208055327U CN 201820377542 U CN201820377542 U CN 201820377542U CN 208055327 U CN208055327 U CN 208055327U
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Abstract
The utility model is related to a kind of hot lid mechanisms of fluorescence quantitative PCR instrument, include the rectangle heat lid of installation base plate and installation thereunder, the surrounding uniform intervals of heat lid offer multiple screw holes, the intermediate position of heat lid is uniformly provided with multiple light holes, the multiple and one-to-one through-hole of screw hole is offered on installation base plate, plug is equipped in each through-hole to screw, plug screws and can be moved up and down in through-hole, it fills in the corresponding screw hole of the precession after through-hole that screws installation base plate and Re Gai are fixed together, each upper correspondence that screws of filling in is set with elastic component, elastic component is between installation base plate and Re Gai.The utility model has the beneficial effects that simple in structure, heat lid is screwed by plug to be fixed together with installation base plate, plug is set with elastic component on screwing, its depth of parallelism between sample tube nozzle is adjusted in elastic component when heat lid and sample tube contact, so that heat lid is in close contact with sample tube nozzle, it is ensured that the accuracy of testing result.
Description
Technical field
The utility model is related to biology and technical field of medical equipment, and in particular to a kind of fluorescence quantitative PCR instrument heat lid
Mechanism.
Background technology
Real-time fluorescence quantitative PCR instrument is made of fluorescent quantitation system and computer, is used in monitoring temperature cyclic process
Fluorescence intensity.The computer being connected with real-time device collects fluorescence data.Data are by the real-time analysis software of exploitation with chart
Form show.Initial data is drawn into chart of the fluorescence intensity relative to recurring number.By can to the analysis of fluorescence intensity
To calculate the DNA profiling content in primary sample.
Real-Time Fluorescent Quantitative PCR Technique has very high sensitivity and prodigious dynamic range, to molecular biology research and
Medical research etc. is of great significance.Heat lid on existing fluorescence quantitative PCR instrument has the following defects：Heat lid is complicated,
There are not parallel phenomenon, the nozzle of sample segment test tube and hot lids when the lower face of heat lid and the nozzle of sample tube contact
Between there are gaps, cause invisible spectro sample to have the phenomenon that evaporation, influence testing result.
Utility model content
The technical problem to be solved by the utility model is to provide a kind of hot lid mechanism of fluorescence quantitative PCR instrument, the hot lid machines
Structure and sample tube nozzle can effectively adjust its depth of parallelism between sample tube nozzle when contacting, it is ensured that sample tube nozzle
It is parallel with hot lid, prevent sample from evaporating, it is ensured that the accuracy of measurement result.
The technical solution that the utility model solves above-mentioned technical problem is as follows：
A kind of hot lid mechanism of fluorescence quantitative PCR instrument includes the rectangle heat lid of installation base plate and installation thereunder, the heat
The surrounding uniform intervals of lid offer multiple screw holes, and the intermediate position of the heat lid is uniformly provided with multiple light holes, the peace
The multiple and one-to-one through-hole of the screw hole is offered on dress substrate, being equipped with plug in each through-hole screws, the plug
Screwing can move up and down in the through-hole, and the plug screws the corresponding screw hole of the precession after the through-hole by institute
It states installation base plate and the heat lid is fixed together, each upper correspondence that screws of filling in is set with elastic component, the elastic component
Between the installation base plate and the heat lid.
The utility model has the beneficial effects that：The corresponding screw hole of the precession after through-hole is screwed by installation base plate by plug
With heat lid be fixed together, heat lid with sample tube nozzle contact when by multiple elastic components adjust itself and sample tube nozzle it
Between the depth of parallelism so that heat lid with sample tube nozzle be in close contact, prevent sample occur Evaporation Phenomenon, it is ensured that measurement result
Accuracy.
Based on the above technical solution, the utility model can also do following improvement.
Further, the elastic component is spring.
Advantageous effect using above-mentioned further scheme be elastic component use spring, it is simple in structure, it is easy to operate, cost compared with
It is low.
Further, the intermediate position of the heat lid is uniformly provided with multiple light holes.
The light for the optical system that heat covers when advantageous effect using above-mentioned further scheme is detection passes through light hole
It is irradiated on sample.
Further, the heat, which covers, is pasted with flexible electrothermal sheet.
Advantageous effect using above-mentioned further scheme is to carry out being heated to set temperature to heat lid by flexible electrothermal sheet.
Further, the flexible electric heating on piece is equipped with thermistor, and the heat, which covers, is equipped with temperature protection switch, institute
It states thermistor and the temperature protection switch is electrically connected with control device.
Advantageous effect using above-mentioned further scheme is to detect the real time temperature of heat lid by thermistor and will correspond to
Temperature signal sends control device to, and control device receives signal simultaneously by temperature control to set temperature, when reaching set temperature
Flexible electrothermal sheet heating power reduces, and temperature is made to keep；When hot lid temperature is more than the design temperature of temperature protection switch, temperature
Protection switching circuit disconnects in time, and flexible electrothermal sheet stops heating, covers heat and carries out duplicate protection；After troubleshooting, temperature
When less than design temperature, temperature protection switch can connect automatically, save the process for replacing fuse.
Description of the drawings
Fig. 1 is the utility model heat lid and the upward view after installation base plate installation；
Fig. 2 is the utility model heat lid and the vertical view after installation base plate installation；
Fig. 3 is the structural schematic diagram of heat lid in the utility model；
Fig. 4 is heat lid and the sectional view after installation base plate installation in the utility model.
In attached drawing, parts list represented by the reference numerals are as follows：
1, installation base plate, 2, Re Gai, 3, screw hole, 4, light hole, 5, through-hole, 6, plug screw, 7, spring, 8, flexible electric heating
Piece, 9, temperature protection switch, 10, thermistor, 11, rectangular through-hole, 12, guide rail, 13, connector, 14, mounting groove.
Specific implementation mode
The principles of the present invention and feature are described below in conjunction with attached drawing, example is served only for explaining this practicality
It is novel, it is not intended to limit the scope of the utility model.
As shown in Figure 1, Figure 2, Figure 3 and Figure 4, the utility model provides a kind of hot lid mechanism of fluorescence quantitative PCR instrument, including peace
The hot lid 2 of rectangle of substrate 1 and installation thereunder is filled, the top of installation base plate 1 is equipped with optical system, and installation base plate 1 uses
The aluminum alloy materials of high thermal conductivity are made, and aluminum alloy surface does anodic oxidation, to increase its anticorrosive property and case hardness.Heat lid
2 surrounding uniform intervals offer multiple screw holes 3, and the intermediate position of hot lid 2 is uniformly provided with multiple light holes 4, installation base plate 1
Upper position corresponding with light hole 4 is opened up there are one the rectangular through-hole 11 used for optical system, and the both sides of rectangular through-hole 11 are pacified
Equipped with the guide rail 12 moved back and forth for optical system；The side of hot lid 2 is connected with the connector 13 for installing electric wire, installation base plate
1 lower surface is equipped with the mounting groove 14 for placing connector 13；Meanwhile multiple and screw hole 3 is offered on installation base plate 1 one by one
Corresponding through-hole 5 is equipped with plug in each through-hole 5 and screws 6, and plug screws and 6 can be moved up and down in through-hole 5, and plug, which screws, 6 to be worn
Installation base plate 1 and Re Gai 2 are fixed together by the corresponding screw hole 3 of precession after crossing through-hole 5, and each plug screws corresponding suit on 6
Flexible part, elastic component is between installation base plate 1 and Re Gai 2.By multiple elasticity when hot lid 2 is contacted with sample tube nozzle
Part adjusts its depth of parallelism between sample tube nozzle so that hot lid 2 is in close contact with sample tube nozzle, prevents sample from sending out
Raw Evaporation Phenomenon, it is ensured that the accuracy of measurement result.
In the utility model, elastic component is preferably spring 7, and spring 7 has a larger elastic deformability, spring 7 by
Power deforms in a linear relationship with it.When hot lid 2 and sample tube nozzle are not parallel, different positions between installation base plate 1 and hot lid 2
7 stress of spring set is of different sizes, and each spring 7 generates corresponding deformation according to itself stress is of different sizes, finally so that heat
Lid 2 and sample tube nozzle keeping parallelism so that hot lid 2 is in close contact with all samples test tube nozzle, it is ensured that testing result
Accuracy.
In the utility model, it is pasted with flexible electrothermal sheet 8 on hot lid 2, thermistor 10 is installed on flexible electrothermal sheet 8,
Temperature protection switch 9 is installed, thermistor 10 and temperature protection switch 9 are electrically connected with control device on hot lid 2.Work as sample
Test tube is sent by temperature control module to corresponding position, and flexible electrothermal sheet 8 begins to warm up hot lid 2, and the temperature of hot lid 2 constantly rises to use
After the temperature (such as 105 DEG C) of family setting, 8 heating power of flexible electrothermal sheet reduces, and start temperature is kept；Flexible electrothermal sheet 8 adds
In thermal process, thermistor 10 detects the real time temperature of hot lid 2 and sends corresponding temperature signal to control device, control dress
Set reception signal.When failure temperature is more than design temperature (such as 125 DEG C) of temperature protection switch 9, temperature protection is opened
It closes 9 to disconnect automatically, flexible electrothermal sheet 8 stops heating, and 2 temperature of hot lid continuously decreases, plays a protective role to hot lid 2, prevent temperature
It spends out of control；After troubleshooting, when temperature is less than design temperature, temperature protection switch 9 can connect automatically, save replacement insurance
The process of silk.
The operation principle of the utility model is as follows：
When experiment, the temperature control module of 2 lower section of hot lid drives sample tube rising to be contacted with hot lid 2, continues to rise 7 quilt of spring
Compression；When hot lid 2 and sample tube nozzle are not parallel, the 7 stress size of multiple springs between installation base plate 1 and hot lid 2 is not
Together, each spring 7 generates corresponding deformation according to itself stress is of different sizes, finally so that hot lid 2 is protected with sample tube nozzle
Maintain an equal level row so that hot lid 2 is in close contact with all samples test tube nozzle, avoids sample that Evaporation Phenomenon occurs, it is ensured that testing result
Accuracy；After sample tube rises in place, flexible electrothermal sheet 8 begins to warm up hot lid 2, and the temperature of hot lid 2 constantly rises, passes through
Algorithm control heating power makes temperature stablize the temperature (105 DEG C) in setting；In 8 heating process of flexible electrothermal sheet, thermistor
The real time temperature of the 10 hot lids of detection 2 simultaneously sends corresponding temperature signal to control device, control device receive signal and with set
Determine temperature comparisons, forms negative-feedback.If there is failure, when temperature control is increased to always the design temperature of protection switch 9, temperature
Degree protection switch 9 disconnects automatically.Flexible electrothermal sheet 8 stops heating, and 2 temperature of hot lid continuously decreases, and plays protection to hot lid 2 and makees
With.
The above is only the preferred embodiment of the present invention, is not intended to limit the utility model, all in this practicality
Within novel spirit and principle, any modification, equivalent replacement, improvement and so on should be included in the guarantor of the utility model
Within the scope of shield.
Claims (5)
1. a kind of hot lid mechanism of fluorescence quantitative PCR instrument, it is characterised in that：Include the square of installation base plate (1) and installation thereunder
The surrounding uniform intervals of shape heat lid (2), the heat lid (2) offer multiple screw holes (3), are offered on the installation base plate (1)
Multiple through-hole (5), the interior plug that is equipped with of each through-hole (5) screw (6) correspondingly with the screw hole (3), and the plug is beaten
Screw (6) can move up and down in the through-hole (5), and the plug screws (6) across the through-hole (5) the corresponding institute of precession afterwards
It states screw hole (3) installation base plate (1) and the heat lid (2) are fixed together, each plug screws corresponding set on (6)
Equipped with elastic component, the elastic component is located between the installation base plate (1) and the heat lid (2).
2. the hot lid mechanism of a kind of fluorescence quantitative PCR instrument according to claim 1, it is characterised in that：The elastic component is bullet
Spring (7).
3. the hot lid mechanism of a kind of fluorescence quantitative PCR instrument according to claim 1, it is characterised in that：In the heat lid (2)
Between position be uniformly provided with multiple light holes (4).
4. the hot lid mechanism of a kind of fluorescence quantitative PCR instrument according to claim 1, it is characterised in that：It is glued on the heat lid (2)
Post flexible electrothermal sheet (8).
5. the hot lid mechanism of a kind of fluorescence quantitative PCR instrument according to claim 4, it is characterised in that：The flexible electrothermal sheet
(8) thermistor (10) is installed on, temperature protection switch (9) is installed on the heat lid (2), the thermistor (10) and
The temperature protection switch (9) is electrically connected with control device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820377542.4U CN208055327U (en) | 2018-03-20 | 2018-03-20 | A kind of hot lid mechanism of fluorescence quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820377542.4U CN208055327U (en) | 2018-03-20 | 2018-03-20 | A kind of hot lid mechanism of fluorescence quantitative PCR instrument |
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| Publication Number | Publication Date |
|---|---|
| CN208055327U true CN208055327U (en) | 2018-11-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820377542.4U Active CN208055327U (en) | 2018-03-20 | 2018-03-20 | A kind of hot lid mechanism of fluorescence quantitative PCR instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114146738A (en) * | 2021-12-02 | 2022-03-08 | 杭州奥盛仪器有限公司 | PCR appearance heat lid, PCR kit and heat lid take off device |
-
2018
- 2018-03-20 CN CN201820377542.4U patent/CN208055327U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114146738A (en) * | 2021-12-02 | 2022-03-08 | 杭州奥盛仪器有限公司 | PCR appearance heat lid, PCR kit and heat lid take off device |
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