CN208043437U - A kind of petty action object table depends on biological sample quick sampling device - Google Patents
A kind of petty action object table depends on biological sample quick sampling device Download PDFInfo
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- CN208043437U CN208043437U CN201820596173.8U CN201820596173U CN208043437U CN 208043437 U CN208043437 U CN 208043437U CN 201820596173 U CN201820596173 U CN 201820596173U CN 208043437 U CN208043437 U CN 208043437U
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Abstract
A kind of petty action object table depends on biological sample quick sampling device, the utility model is related to a kind of biological samples to sample equipment, the utility model is to solve existing animal body surface to depend on that biological sampling method is long there are the sampling time, sampling quantity is few, fixation of animal is difficult, animal is in stress situation for a long time, it be easy to cause the damage to animal, the easily infected problem of sample collector.The utility model includes Baoding bag and sampler bag.Baoding bag is lighttight unidirectional open-bag of breathing freely, and sack sets draw-string to collect sack.Sampler bag is two-way opened pocket, and suitable for reading to set draw-string to collect sack, lower mouth sets a plurality of closing concave convex button, and lower section suitable for reading, which is set, can close sample solution inlet.Baoding bag is setting up and down with sampler bag, and sack draw-string set position is arranged in sampler bag, and sack draw-string set is located at draw-string set suitable for reading.The utility model depends on biological sample for acquiring petty action object table.
Description
Technical field
The utility model is related to a kind of biological samples to sample equipment, and in particular to a kind of petty action object table depends on biological sample
Quick sampling device.
Background technology
It includes epizoon, the bacterium of adherency, fungi and virus etc. that animal body surface, which depends on biology,.These biology with institute according to
There is complicated interaction relationship between attached animal, there is potential great influence to animal body and environmental organism diversity.This
Microorganism in a little biologies and viral monoid, may lead to that people and animals, people fowl is total to illness leads to infectious disease by the activity of animal
It is popular.Therefore, it is zoology, ecology, the important object of epidemiological study and epidemic disease source epidemic disease that animal body surface, which depends on biology,
The key content of monitoring.
Traditional animal body surface depends on biological sampling method, applied to microorganism continuous swab method, be applied to
The brush of parasite sweeps method etc., or the directly cutaneous appendages such as clip animal body surface tissue or hair, feather, scale.These sides
Method is the most outstanding the disadvantage is that, the sampling time is long, and fixation of animal is difficult, and animal is in stress situation for a long time, be easy to cause
Damage to animal is abandoned by international mainstream animal protection idea.In addition, these method of samplings, which are adopted, is limited to animal body surface office
Portion's range, it is difficult in the case of not damaged animal, disposably acquire enough biological samples.At the same time, sample collector needs
It to be in direct contact with animal, increase the difficulty of sampling protection, easily cause the infection to sample collector, it is strong to endanger sample collector
Health.
Utility model content
The utility model is to solve existing animal body surface to depend on that biological sampling method is long there are the sampling time, sampling quantity is few,
Fixation of animal is difficult, and animal is in stress situation for a long time, be easy to cause the damage to animal, and sample collector is easily infected to be asked
Topic provides a kind of petty action object table and depends on biological sample quick sampling device.
A kind of petty action object table depends on biological sample quick sampling device, and composition includes Baoding bag and sampler bag, Baoding
The lower end of bag is equipped with sack, and sack draw-string set is equipped at sack, sack draw-string is installed in sack draw-string set；
The upper end of the sampler bag is suitable for reading equipped with sampler bag, and the lower end of the sampler bag is equipped with mouth under sampler bag, described to adopt
Sample bag place suitable for reading is equipped with draw-string set suitable for reading, and draw-string suitable for reading is installed in draw-string set suitable for reading, and draw-string suitable for reading set lower section is located on sampler bag
If sample solution inlet to be opened/closed, concave convex button is equipped under the sampler bag at mouth；
Baoding bag is setting up and down with sampler bag, and sack draw-string set position is arranged in sampler bag, and sack draw-string set is located at
At draw-string set suitable for reading.
The utility model has the advantages that compared with prior art：
One, it is sampled using the device of the utility model, solves that conventional method unitary sampling amount is few, and the sampling time is long
The problem of.The effect of opening is sampler bag simultaneously above and below, and animal is placed in sampler bag from bottom to top, can be to avoid because of animal
It scratches to take off and kicks caused the problem of being difficult to animal being placed in sampler bag, a plurality of concave convex button can easily close mouth under sampler bag.
Sample solution can be injected sampler bag easily and fast, save processing time by closed sample solution inlet.Baoding bag will move
Object head is placed in dark surrounds, Animal stress state of releiving, and mitigates the intensity that animal struggles, when not only improving shortening sampling
Between, it is also ensured that animal welfare.Because the use of object being toy, using this Baoding method, anesthesia hand can not used
Section avoids anesthesia injury caused by animal.Simultaneously as animal is closed in sampler bag when sampling, sampling people is avoided
Member and being in direct contact before sample can provide more safeguard procedures to sample collector, ensure the safety of sample collector.
Two, the apparatus structure of the utility model is simple, of low cost, easy to use.Whole device uses cloth and plastics
It is made, it is of low cost.Such as it is applied to acquisition micro-biological samples, can be cleaned when making, sterilizing, sterile suit is made.Such as application
In acquisition parasite specimen, then rear Reusability capable of washing, avoids wasting, easy to utilize.
Description of the drawings
Fig. 1 is that a kind of petty action object table of the utility model depends on the overall structure signal of biological sample quick sampling device
Figure；
Fig. 2 is that realize that petty action object table depends in biological sample quick sampling method using the utility model small in step 3
Schematic diagram after fixation of animal；
Fig. 3 is to realize that petty action object table depends in biological sample quick sampling method bird in step 4 using the utility model
Class toy is placed in the schematic diagram in sampler bag 2；
Fig. 4 is to realize that petty action object table depends in biological sample quick sampling method mouse in step 4 using the utility model
Class toy is placed in the schematic diagram in sampler bag 2.
Specific implementation mode
Specific implementation mode one：Illustrate that present embodiment, present embodiment are a kind of petty action object table in conjunction with Fig. 1 and Fig. 3
Biological sample quick sampling device is depended on, composition includes Baoding bag 1 and sampler bag 2, and the lower end of Baoding bag 1 is equipped with sack 1-3,
It is equipped with sack draw-string at sack 1-3 and covers 1-2, sack draw-string 1-1 is installed in sack draw-string set 1-2；
The upper end of the sampler bag 2 is equipped with sampler bag 2-1 suitable for reading, and the lower end of sampler bag 2 is equipped with mouth 2-6 under sampler bag, adopts
It is equipped with draw-string suitable for reading at sample bag 2-1 suitable for reading and covers 2-2, is installed with draw-string 2-3 suitable for reading in draw-string set 2-2 suitable for reading, is located on sampler bag 2 upper
Mouth draw-string, which covers, sets sample solution inlet 2-4 to be opened/closed below 2-2, concave convex button 2-5 is equipped at mouth 2-6 under sampler bag；
Baoding bag 1 is setting up and down with sampler bag 2, and the positions sack draw-string set 1-2 are arranged in sampler bag 2, sack draw-string
Set 1-2 is located at draw-string suitable for reading set 2-2.
Sample solution inlet 2-4 be repeatedly can opening and closing structure, opening can inject sample solution, and closing post-sampling liquid cannot be through this
Mouth outflow.
Sample solution inlet 2-4 can also be closed with materials such as soft rubbers, inject sample solution, extraction using injector for medical purpose
After syringe, inlet 2-4 natural enclosings.
Closing can be repeatedly opened and closed with concave convex button 2-5, mouth 2-6 toy can be put into sampler bag 2 under sampled bag when unlatching
In, when closing, can prevent mouth 2-6 under the sampled bag of sample solution from flowing out.
Closing may be used diversified forms, including slide fastener, double faced adhesive tape etc. with concave convex button 2-5 and may be implemented repeatedly to be opened and closed simultaneously
The structure of mouth 2-6 under sampler bag can be closed, concave convex button 2-5 can be one or more.It can also use under inflection sampler bag
Mouth 2-6, with mouth under the fixed form closing sampler bag of clip.
Specific implementation mode two：Embodiment is described with reference to Fig. 1, present embodiment be Baoding bag 1 by black, it is ventilative,
Opaque material is made.Other compositions and connection relation are same as the specific embodiment one.
Specific implementation mode three：Embodiment is described with reference to Fig. 1, and present embodiment is sampler bag 2 by transparent, waterproof
Material is made.Other compositions and connection relation are the same as one or two specific embodiments.
Realize that petty action object table depends on biological sample quick sampling method using the utility model：See Fig. 1~Fig. 4,
Step 1: preparing sample solution：According to sampling biological characteristic, required sample solution is selected, such as PBS buffer solution, sterile
Water or 5% surfactant solution etc., by sample solution preheating and it is consistent with animal heat；
Step 2: preparing sampler bag 2：Baoding bag 1 sack draw-string 1-1 and draw-string 2-3 suitable for reading of sampler bag 2 is unclamped, is beaten
Open concave convex button 2-5；
Step 3: Baoding toy：Baoding bag 1 is sleeved on toy head, and sack 1-3 is collected to toy neck
Portion keeps Baoding bag 1 not only not easily to fall off, but also will not influence animal breath, takes out the sack draw-string 1-1 of tight Baoding bag 1,
Step 4: toy is placed in sampler bag 2：Toy head behind Baoding is upward, under the sampler bag by sampler bag 2
In mouth 2-6 merging sampler bags 2, toy head is pierced by sampler bag 2-1 suitable for reading and toy neck is made to be located at draw-string suitable for reading set 2-2
Place, takes out the draw-string 2-3 suitable for reading of tight sampler bag 2, keeps sampler bag 2 not only not easily to fall off, but also will not influence animal breath, closes sampler bag
Whole concave convex button 2-5 of 2 times mouths；
Step 5: sampling：Sample solution after being preheated in step 1 is injected into 10ml~30ml by sample solution inlet 2-4,
Sample solution inlet 2-4 is closed, 2 one way or another slight oscillatory 3min~5min of sampler bag is held, petty action object table is made to depend on
Biology is fallen into sample solution, that is, obtains sample suspensions；
Step 6: release toy：Draw-string 2-3 suitable for reading is unclamped, sampled bag 2-1 suitable for reading takes out toy, cleans petty action
Object, dry toy hair or feather, unclamp the sack draw-string 1-1 of Baoding bag 1, discharge toy；
Step 7: sample liquid is handled：The sampler bag 2-1 suitable for reading of sampled bag 2 pours out sample liquid, filters sample liquid
Obtain required biological sample.
If what is acquired is the fungi of birds, Step 2: three, four, five and six can take sterile working；And the sample in step 7
This liquid handling mode is：Sample liquid is poured out, inside 5ml PBS buffer solution rinsing sampler bag 2, the liquid after rinsing and sample
This liquid mixes, that is, obtains sample suspensions, filters sample mixed liquor using bacterial filter, fungi sample is enriched on filter membrane,
It obtains petty action object table and depends on biological sample.
If what is acquired is the hard tick of muroid, sample solution selects civilian liquid detergent in step 1；And the sample liquid in step 7
Body processing mode is：Sample liquid is poured out, sample liquid is filtered using ordinary filter paper, the hard tick that will be left on filter paper is packed into sample
Pipe obtains petty action object table and depends on biological sample.
The application example of the utility model：
Embodiment 1：
Picoides major body surface fungi sample is acquired, to analyze Picoides major whether can be between health tree and disease tree
Propagate fungi.Sampling step is as follows：
Step 1: due to acquisition being fungi, subsequent processing is formed using high-flux sequence analysis fungi, so selection
The PBS buffer solution of pH7.5 is preheated to 42 DEG C；
Step 2: take half sterile working, unclamp the sack draw-string 1-1 of Baoding bag 1, the draw-string 2-3 suitable for reading of sampler bag 2,
The lower mouth closing concave convex button 2-5 of separation whole sampler bag 2；
Step 3: capturing Picoides major using mist net, Baoding bag 1 is sleeved on its head, appropriateness takes out the bag of tight Baoding bag 1
Mouth draw-string 1-1 collects the sack 1-3 of Baoding bag 1 to Picoides major neck, keeps Baoding bag 1 not only not easily to fall off, but also will not shadow
It rings it and breathes (see Fig. 2), do so sample collector when can also avoid subsequent operation and inhaled by bird and hindered, both protect animal, also protect
The safety of sample collector is protected；
Step 4: the Picoides major head behind Baoding is upward, mouth 2-6 is placed in sampler bag 2 under sampled bag, and head
Portion is pierced by sampler bag 2-1 suitable for reading, and appropriateness takes out the draw-string 2-3 suitable for reading of tight sampler bag 2, makes sampler bag is suitable for reading to collect to neck, make to adopt
Sample bag 2 was not only not easily to fall off, but also did not influenced Picoides major breathing, closed mouth whole concave convex button 2-5 under sampler bag (see Fig. 3)；
Step 5: sampled liquid inlet 2-4 injections 20ml is preheated to 42 DEG C of PBS buffer solution, closing sample solution injection
Mouth 2-4, holds and holds sampler bag 2, keeps Picoides major head upward, one way or another slight oscillatory 3min makes its body surface depend on
Fungi fall into sample solution；
Step 6: sampling terminates, the draw-string 2-3 suitable for reading of sampler bag 2 is unclamped, Picoides major is taken out, with being preheated to 42 DEG C
Clear water rinse animal after dry feather, unclamp the sack draw-string 1-1 of Baoding bag 1, discharge Picoides major；
Step 7: sampled bag 2-1 suitable for reading pours out sample solution, and inside the above-mentioned PBS bufferings rinsing sampler bags 2 of 5ml, rinsing
Liquid is merged into aforementioned sample liquid.Using bacterial filter sampling by filtration liquid, fungi sample is enriched on filter membrane, sequencing company is sent
Carry out high-flux sequence.
Embodiment 2：
Acquire the hard tick sample of Siberian chipmunk body surface.Sampling step is as follows：
Step 1: due to acquisition being hard tick, seeks connections on Siberian chipmunk hair, theirs can be weakened using surfactant
Power is sought connections with, it is made to fall off from animal body surface.The present embodiment uses 10% commercially available civilian liquid detergent as sample solution, is preheated to 37
℃；
Step 2: due to the animal specimen of acquisition, sterile working is not needed, the sack draw-string 1- of Baoding bag 1 is directly unclamped
1, the lower mouth closing concave convex button 2-5 of the draw-string 2-3 suitable for reading of sampler bag 2, separation whole sampler bag 2；
Step 3: cage catches Siberian chipmunk, Baoding bag 1 is sleeved on Siberian chipmunk head, appropriateness takes out the sack draw-string 1-1 of tight Baoding bag 1, will
The sack 1-3 of Baoding bag 1 is collected to Siberian chipmunk neck, keeps Baoding bag 1 not only not easily to fall off, but also will not be influenced it and be breathed (see Fig. 4)；
Step 4: the Siberian chipmunk head behind Baoding is upward, mouth 2-6 is placed in sampler bag 2 under sampled bag, and head is pierced by
Sampler bag 2-1 suitable for reading, appropriateness take out the draw-string 2-3 suitable for reading of tight sampler bag 2, make sampler bag is suitable for reading to collect to neck, made sampler bag 2 both
It is not easily to fall off, and Siberian chipmunk breathing is not influenced, close mouth whole concave convex button under sampler bag (see Fig. 4)；
Step 5: sampled liquid inlet 2-4 injections 20ml is preheated to 37 DEG C of 10% cleanser essence, closing sample solution note
Entrance 2-4 holds and holds sampler bag 2, keeps Siberian chipmunk head upward, one way or another slight oscillatory 5min makes its body surface depend on hard
Tick is fallen into sample solution；
Step 6: sampling terminates, the draw-string 2-3 suitable for reading of sampler bag 2 is unclamped, Siberian chipmunk is taken out, with the clear water for being preheated to 37 DEG C
Hair is dried after rinsing animal, the sack draw-string 1-1 of Baoding bag 1 is unclamped, discharges Siberian chipmunk；
Step 7: sampled bag 2-1 suitable for reading pours out sample solution, using ordinary filter paper sampling by filtration liquid, transfer stays in filter paper
On hard tick to specimen tube.
Above embodiment is only that specific embodiment of the present utility model is described, not to the utility model
Range be defined, those skilled in the art can also do numerous modifications and variations on the basis of existing technology, not depart from
Under the premise of the spirit of the design of the utility model, those of ordinary skill in the art make the technical solution of the utility model various
Variations and modifications should all be fallen into the protection domain of claims determination of the utility model.
Claims (3)
1. a kind of petty action object table depends on biological sample quick sampling device, it is characterised in that：The sampling apparatus includes Baoding
The lower end of bag (1) and sampler bag (2), Baoding bag (1) is equipped with sack (1-3), and sack draw-string set (1-2) is equipped at sack (1-3),
Sack draw-string (1-1) is installed in sack draw-string set (1-2)；
The upper end of the sampler bag (2) is (2-1) suitable for reading equipped with sampler bag, and the lower end of the sampler bag (2) is equipped with mouth under sampler bag
(2-6) is equipped with draw-string set (2-2) suitable for reading at the sampler bag (2-1) suitable for reading, draw-string suitable for reading is installed in draw-string set (2-2) suitable for reading
(2-3) is located on sampler bag (2) below draw-string set (2-2) suitable for reading and sets sample solution inlet (2-4) to be opened/closed, the sampling
It is equipped with concave convex button (2-5) at the lower mouth (2-6) of bag；
Baoding bag (1) and sampler bag (2) are setting up and down, and the position sack draw-string set (1-2) is arranged in sampler bag (2), sack
Draw-string set (1-2) is located at draw-string set (2-2) suitable for reading.
2. a kind of petty action object table according to claim 1 depends on biological sample quick sampling device, it is characterised in that：Institute
Baoding bag (1) is stated to be made of black, ventilative, opaque material.
3. a kind of petty action object table according to claim 1 or 2 depends on biological sample quick sampling device, feature exists
In：The sampler bag (2) is made of transparent, impermeable material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820596173.8U CN208043437U (en) | 2018-04-25 | 2018-04-25 | A kind of petty action object table depends on biological sample quick sampling device |
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| CN201820596173.8U CN208043437U (en) | 2018-04-25 | 2018-04-25 | A kind of petty action object table depends on biological sample quick sampling device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108267330A (en) * | 2018-04-25 | 2018-07-10 | 东北林业大学 | A kind of petty action object table depends on biological sample quick sampling device and method |
-
2018
- 2018-04-25 CN CN201820596173.8U patent/CN208043437U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108267330A (en) * | 2018-04-25 | 2018-07-10 | 东北林业大学 | A kind of petty action object table depends on biological sample quick sampling device and method |
| CN108267330B (en) * | 2018-04-25 | 2023-06-23 | 东北林业大学 | Device and method for rapidly sampling biological samples attached to body surfaces of small animals |
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| GR01 | Patent grant | ||
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| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20181102 Effective date of abandoning: 20230623 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20181102 Effective date of abandoning: 20230623 |