CN101914610A - Pig breeding and respiratory syndrome sampling method - Google Patents
Pig breeding and respiratory syndrome sampling method Download PDFInfo
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- CN101914610A CN101914610A CN2010102402275A CN201010240227A CN101914610A CN 101914610 A CN101914610 A CN 101914610A CN 2010102402275 A CN2010102402275 A CN 2010102402275A CN 201010240227 A CN201010240227 A CN 201010240227A CN 101914610 A CN101914610 A CN 101914610A
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- sliver
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- pig
- cotton sliver
- respiratory syndrome
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Abstract
The invention relates to a pig breeding and respiratory syndrome sampling method. A tough cotton sliver is made, the cotton sliver is coated and dipped with inducer, the cotton sliver is fixed on a water drinking device in pig farm colony house, a pig can bite the cotton sliver when drinking water and spittle is adhered on the cotton silver, when the cotton sliver is adhered with spittle, the cotton sliver is taken down and placed into a sample bag, then spittle in the cotton sliver is extruded out, the lower end of the sample bag is opened with an aperture in a tapering way, so that sample is dropwise added into a sample tube along with the aperture, and the sample is refrigerated at 4 DEG C, thus finishing the sampling process. The invention has the advantages that: 1, no restraint person is required, no stress reaction of pig can be induced, no nasal cavity damage on pig can be produced; 2, manpower is saved, operation can be carried out by only one person; 3, the whole process is simple and easy to operate, operation can be carried out only by simple training; 3, one sample is collected in each pigsty and can be on behalf of the whole pigs in the pigsty.
Description
Technical field
The present invention relates to a kind of method of sampling, relate in particular to a kind of pig breeding and respiratory syndrome sampling method.
Background technology
All the time, generally adopt serum sample to detect antibody, finish by detection meanss such as ELISA, radioimmunities for the detection of animal epidemic.But detect antibody can only confirm that this animal once infected should disease, can't learn that this animal is premunition or is infecting.Continuous development along with the quarantine technology for some porcine respiratory class eqpidemic diseases, as porcine reproductive and respiratory syndrome, directly is positioned detection of nucleic acids at present; If detect nucleic acid, prove that then this virus exists in this animal body; The sample of respiratory tract eqpidemic disease detection of nucleic acids is generally nasal cavity swab sample.The process of nasal cavity swab sampling is: need 2 Baoding personnel, 1 sample collecting personnel; In the time of personnel Baoding, Baoding, the sample collecting personnel inject the nasal cavity of pig with cotton swab, respectively change 3 circles more clockwise, counterclockwise, then put in the test tube that physiological saline or PBS are housed, and sealing and cryopreservation are sent to the laboratory and detect.This process is numerous and diverse, the technical requirements height, and cause pig stress reaction and nasal cavity infringement only easily; The method lost labor in Baoding, requiring skilled workman and animal doctor personnel to cooperatively interact just can finish, and have a lot of shortcomings simultaneously: pig differ in disposition only, in the process in Baoding, can have pernicious habits such as stinging, play, support, not only cause pig only oneself injury but also can animal doctor and workman's personnel safety be threatened.
Summary of the invention
The object of the present invention is to provide a kind of pig breeding and respiratory syndrome sampling method, this method of sampling has solved in traditional pig breeding and the respiratory syndrome sampling method needs Baoding personnel and animal doctor to cooperate the problem that could sample, directly do not contact simultaneously, guaranteed animal doctor and Baoding personnel's personnel safety with pig.
The present invention is achieved like this, it is characterized in that method is: make a tough and tensile sliver, be coated with inductor on the sliver, sliver after making is fixed on the drinking device of pig farm colony house, when pig drinks water, use face and sting sliver, saliva sticks on the sliver simultaneously, after being stained with saliva on the sliver, sliver is taken off, and be loaded in the sample sack, extrude the saliva in the sliver then, gradually open an aperture in the lower end of sample sack, sample is splashed in the sample hose along aperture, and to 4 ℃ of refrigerations, sampling process finishes.
Described tough and tensile sliver is made and sliver can be fixed on the pole with cord.
Advantage of the present invention is: 1, do not need Baoding personnel, can not cause only stress reaction of pig, can not cause pig nasal cavity damage only; 2, save manpower, only need a people to operate; 3, whole process is simple, only needs simple training to get final product complete operation; 3, each swinery is gathered a sample, can represent in this swinery all pigs only.
Embodiment
The present invention is achieved like this, method is: sliver is fixed on the pole with cord, be coated with inductor on the sliver, sliver after making is fixed on the drinking device of pig farm colony house, when pig drinks water, use face and sting sliver, saliva sticks on the sliver simultaneously, after being stained with saliva on the sliver, sliver is taken off, and be loaded in the sample sack, extrude the saliva in the sliver then, gradually open an aperture in the lower end of sample sack, sample is splashed in the sample hose along aperture, and to 4 ℃ of refrigerations, sampling process finishes.Each swinery is gathered a sample, and this sample can represent all pigs of this swinery only.
Claims (2)
1. pig breeding and respiratory syndrome sampling method, it is characterized in that method is: make a tough and tensile sliver, be coated with inductor on the sliver, sliver after making is fixed on the drinking device of pig farm colony house, when pig drinks water, use face and sting sliver, saliva sticks on the sliver simultaneously, after being stained with saliva on the sliver, sliver is taken off, and be loaded in the sample sack, extrude the saliva in the sliver then, gradually open an aperture in the lower end of sample sack, sample is splashed in the sample hose along aperture, and to 4 ℃ of refrigerations, sampling process finishes.
2. pig breeding according to claim 1 and respiratory syndrome sampling method is characterized in that described tough and tensile sliver making can be fixed on sliver on the pole with cord.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102402275A CN101914610A (en) | 2010-07-29 | 2010-07-29 | Pig breeding and respiratory syndrome sampling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102402275A CN101914610A (en) | 2010-07-29 | 2010-07-29 | Pig breeding and respiratory syndrome sampling method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101914610A true CN101914610A (en) | 2010-12-15 |
Family
ID=43322247
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102402275A Pending CN101914610A (en) | 2010-07-29 | 2010-07-29 | Pig breeding and respiratory syndrome sampling method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101914610A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103558058A (en) * | 2013-11-05 | 2014-02-05 | 山东省动物疫病预防与控制中心 | Pig saliva sampling device |
| CN104428428A (en) * | 2012-09-06 | 2015-03-18 | 英国西门子公司 | Dry slag granulation system |
| CN106614265A (en) * | 2016-09-19 | 2017-05-10 | 赵国启 | Flavored commercial pig breeding method |
-
2010
- 2010-07-29 CN CN2010102402275A patent/CN101914610A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104428428A (en) * | 2012-09-06 | 2015-03-18 | 英国西门子公司 | Dry slag granulation system |
| CN104428428B (en) * | 2012-09-06 | 2016-10-19 | 英国西门子公司 | Dry slag granulation system |
| CN103558058A (en) * | 2013-11-05 | 2014-02-05 | 山东省动物疫病预防与控制中心 | Pig saliva sampling device |
| CN106614265A (en) * | 2016-09-19 | 2017-05-10 | 赵国启 | Flavored commercial pig breeding method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20101215 |