CN208038440U - A kind of tissue freeze grinding device - Google Patents
A kind of tissue freeze grinding device Download PDFInfo
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- CN208038440U CN208038440U CN201820012611.1U CN201820012611U CN208038440U CN 208038440 U CN208038440 U CN 208038440U CN 201820012611 U CN201820012611 U CN 201820012611U CN 208038440 U CN208038440 U CN 208038440U
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- grinding
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- tissue
- accommodating cavity
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Abstract
The utility model discloses a kind of tissue freeze grinding devices, it is desirable to provide a kind of tissue freeze grinding device of simple operation.It includes the grinding pot that middle part has grinding hole, and grinding rod compatible with grinding hole；The tank body of the grinding pot is double layer hollow structure, and hollow space forms the accommodating cavity of accommodating liquid nitrogen；The open upper end of the accommodating cavity, and the lid of upper end setting closing the upper end of accommodating cavity；There is the through-hole passed through for grinding rod in the middle part of the lid.The utility model is simple in structure, easy to use, can effectively ensure that safety and the validity of experiment.
Description
Technical field
The utility model is related to freezing tissue milling apparatus technical fields, are filled more particularly, to a kind of tissue freeze grinding
It sets.
Background technology
In the related experiment of tissue RNA extractions, often needs under cryogenic to be ground tissue, become powder
Last shape carries out next step experiment again.Current operation is usually：Experimenter pours into liquid nitrogen in common mortar, then from liquid nitrogen
It takes out tissue to be ground and is put into mortar, be ground with grinding rod.Since common mortar opening is larger, liquid nitrogen volatilization compared with
Soon, it is therefore desirable to liquid nitrogen is added while grinding, and liquid nitrogen belongs to the liquid of ultralow temperature, having in continuous toppling process can
Energy splash is to cause experimenter the possibility of frostbite.In addition, in process of lapping, since some tissues are of great rarity, only
There is the particle of very little, if grinding rod pressure is improper when grinding, tissue particles can be caused to fly out outside mortar, to cause
The degradation of RNA in tissue.Since the usual bottom of mortar is circle, when addition Trizol extracts RNA after grinding
More liquid residual is had to cause to waste.
For the problem present on, the solution that experimenter mainly uses at present is to select opening is smaller to grind
Alms bowl, but cause the amount of liquid nitrogen that can be stored too small again in this way, to need that liquid nitrogen is ceaselessly added.If to mortar mouth lid into
Row closing, and lead to the operation for influencing grinding rod.
Utility model content
The purpose of this utility model is intended to overcome the shortcomings of the prior art, and it is low to provide a kind of liquid nitrogen loss, operation
Safe and convenient tissue freeze grinding device.
In order to solve the above-mentioned technical problem, the utility model is achieved through the following technical solutions：
A kind of tissue freeze grinding device, including middle part have the grinding pot of grinding hole, and compatible with grinding hole
Grinding rod；The tank body of the grinding pot is double layer hollow structure, and hollow space forms the accommodating cavity of accommodating liquid nitrogen；The accommodating cavity
Open upper end, and accommodating cavity upper end setting closing the upper end lid；Have in the middle part of the lid and to be passed through for grinding rod
Through-hole.
Preferably, the grinding hole is cylindrical, and bottom is in cone shape.
Preferably, the grinding rod includes milling bar, is set to the grinding head of milling bar one end, and is set to grinding
The grinding handle of the bar other end.
Preferably, it is provided with sealing ring at the lid and accommodating cavity overlap joint closing.
Preferably, the handle that setting opens and closes convenient for lid on the lid.
Compared with prior art, the utility model has the following advantages that：
（1）The utility model is simple in structure, easy to use, solves in tissue sample process of lapping, since liquid nitrogen is waved
Hair results in the need for the Operating Complexity for not stopping that liquid nitrogen is added, and maintains the low temperature environment of tissue sample in process of lapping, keeps away
Exempted from during tissue grinder experimenter's frostbite caused by liquid nitrogen or tissue splash may with the degradation of tissue RNA, to
It ensure that safety and the validity of experiment.
（2）Grinding and addition Trizol of the abrading tip mode of the utility model setting especially suitable for ultramicron sample
Later collection process, substantially increase the conventional efficient of trace sample.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural schematic diagram of the utility model one embodiment.
Fig. 2 is the structural schematic diagram of second embodiment of the utility model.
Fig. 3 is the structural schematic diagram of the utility model third embodiment.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are not under the premise of making the creative labor
The every other embodiment obtained, shall fall within the protection scope of the present invention.
Tissue freeze grinding device shown in FIG. 1, include middle part have grinding hole 1 grinding pot 2, and with grinding hole 1
Compatible grinding rod 3；The tank body of the grinding pot 2 is double layer hollow structure, and hollow space forms the accommodating of accommodating liquid nitrogen
Chamber 4；The open upper end of the accommodating cavity 4, and the lid 5 of upper end setting closing the upper end of accommodating cavity 4；5 middle part tool of the lid
There is the through-hole 6 passed through for grinding rod 3.
In the present embodiment, the grinding hole 1 is cylindrical, and bottom is in cone shape, in this way can be right in spin finishing
Micro-example plays good pulverization, while being wasted caused by liquid residual when reducing extraction.Obvious grinding hole 1 can also be adopted
With other applicable shapes.
Wherein, the grinding rod 3 includes milling bar 31, is set to the grinding head 32 of 31 one end of milling bar, and be set to
The grinding handle 33 of 31 other end of milling bar.Other applicable structure types also can be used in obvious milling bar 31.
As a further improvement, the lid 5 overlaps with accommodating cavity 4 and is provided with sealing ring 7 at closing, to improve sealing
Property.
Freeze grinding device is organized shown in shown in Fig. 2, wherein the handle that setting opens and closes convenient for lid 5 on the lid 5
8, which is ring-shaped handle, it is clear that the handle of other forms also can be used.Remaining is essentially identical with embodiment shown in FIG. 1.
Freeze grinding device is organized shown in shown in Fig. 3, wherein use the grinding hole of another structure type, remaining with
Embodiment shown in FIG. 1 is essentially identical.
The accommodating cavity 4 of the utility model can pour into more liquid nitrogen, and liquid nitrogen can seal liquid nitrogen region with lid 5 after pouring into
It closes, there is sealing ring 7 between lid 5 and grinding device, it is possible to reduce liquid nitrogen volatilizees.4 inner wall of accommodating cavity, which uses, leads the good material of temperature
Material, micro tissue to be ground can be put into grinding hole 1, rotatably be ground with grinding rod 3, the bottom shape of grinding rod 3 with
Grinding hole 1 agrees with, and 1 hole wall of grinding hole is ground with the surface of grinding rod 3 using the rough surface of similar frosted.Knot to be ground
Appropriate Trizol is added in Shu Hou, is sucked out again after being mixed well with the powder after grinding and moves into centrifuge tube progress next step experiment.Such as
Fruit is ultramicron tissue, can prepare with the sizeable centrifuge tube in 1 bottom of grinding hole, centrifuge tube is put into grinding hole 1, then will
Tissue is put into centrifuge tube, then is ground with grinding rod 3, and Trizol is directly added in centrifuge tube after to be ground, is mixed
Centrifuge tube is directly taken out after even carries out next step experiment.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Within the spirit and principle of utility model, any modification, equivalent replacement, improvement and so on should be included in the utility model
Protection domain within.
Claims (5)
- Include the grinding pot that middle part has grinding hole 1. a kind of tissue freeze grinding device, and compatible with grinding hole grinds Frotton；It is characterized in that：The tank body of the grinding pot is double layer hollow structure, and hollow space forms the accommodating of accommodating liquid nitrogen Chamber；The open upper end of the accommodating cavity, and the lid of upper end setting closing the upper end of accommodating cavity；Have for grinding in the middle part of the lid The through-hole that frotton passes through.
- 2. organizing freeze grinding device according to claim 1, it is characterised in that：The grinding hole is cylindrical, bottom In cone shape.
- 3. tissue freeze grinding device according to claim 1 or claim 2, it is characterised in that：The grinding rod includes milling bar, if It is placed in the grinding head of milling bar one end, and is set to the grinding handle of the milling bar other end.
- 4. tissue freeze grinding device according to claim 1 or claim 2, it is characterised in that：The lid and accommodating cavity lap jointss The place of closing is provided with sealing ring.
- 5. tissue freeze grinding device according to claim 1 or claim 2, it is characterised in that：Lid is convenient in setting on the lid The handle of keying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820012611.1U CN208038440U (en) | 2018-01-04 | 2018-01-04 | A kind of tissue freeze grinding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820012611.1U CN208038440U (en) | 2018-01-04 | 2018-01-04 | A kind of tissue freeze grinding device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208038440U true CN208038440U (en) | 2018-11-02 |
Family
ID=63956367
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820012611.1U Active CN208038440U (en) | 2018-01-04 | 2018-01-04 | A kind of tissue freeze grinding device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208038440U (en) |
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2018
- 2018-01-04 CN CN201820012611.1U patent/CN208038440U/en active Active
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