CN107904153A - One kind tissue freeze grinding device - Google Patents
One kind tissue freeze grinding device Download PDFInfo
- Publication number
- CN107904153A CN107904153A CN201810009041.5A CN201810009041A CN107904153A CN 107904153 A CN107904153 A CN 107904153A CN 201810009041 A CN201810009041 A CN 201810009041A CN 107904153 A CN107904153 A CN 107904153A
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- CN
- China
- Prior art keywords
- grinding
- lid
- hole
- accommodating cavity
- grinding device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000227 grinding Methods 0.000 title claims abstract description 70
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 19
- 239000011796 hollow space material Substances 0.000 claims abstract description 4
- 238000003801 milling Methods 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 5
- 210000001503 joint Anatomy 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 6
- 239000004570 mortar (masonry) Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 3
- 101100298225 Caenorhabditis elegans pot-2 gene Proteins 0.000 description 2
- 208000001034 Frostbite Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000002123 RNA extraction Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses one kind to organize freeze grinding device, it is desirable to provide a kind of tissue freeze grinding device of simple operation.It includes the grinding pot that middle part has grinding hole, and the grinding rod being adapted with grinding hole；The tank body of the grinding pot is double layer hollow structure, its hollow space forms the accommodating cavity of accommodating liquid nitrogen；The open upper end of the accommodating cavity, and the upper end of accommodating cavity sets the lid of closing the upper end；There is the through hole passed through for grinding rod in the middle part of the lid.It is the configuration of the present invention is simple, easy to use, it can effectively ensure that the security and validity of experiment.
Description
Technical field
The present invention relates to freezing tissue milling apparatus technical field, and freeze grinding device is organized more particularly, to one kind.
Background technology
In the related experiment of tissue RNA extractions, often need under cryogenic to be ground tissue, become powder
Last shape carries out next step experiment again.Current operation is usually：Experimenter pours into liquid nitrogen in common mortar, then from liquid nitrogen
Take out tissue to be ground and be put into mortar, be ground with grinding rod.Since common mortar opening is larger, liquid nitrogen volatilization compared with
It hurry up, it is therefore desirable to grind while adding liquid nitrogen, and liquid nitrogen belongs to the liquid of ultralow temperature, having in continuous toppling process can
Energy splash is so as to cause experimenter the possibility of frostbite.In addition, in process of lapping, since some tissues are of great rarity, only
There is the particle of very little, if grinding rod pressure is improper when grinding, tissue particles can be caused to fly out outside mortar, so as to cause
The degraded of RNA in tissue.Since the usual bottom of mortar is circle, when addition Trizol extracts RNA after grinding
More liquid residue is had to cause to waste.
For the problem present on, the solution that experimenter mainly uses at present is to select opening is less to grind
Alms bowl, but so cause the amount of liquid nitrogen that can store too small again, so as to need ceaselessly to add liquid nitrogen.If to mortar mouth lid into
Row closing, and cause the operation for influencing grinding rod.
The content of the invention
The purpose of the present invention is intended to overcome the shortcomings of the prior art, there is provided a kind of liquid nitrogen loss is low, safe operation
Property high and convenient tissue freeze grinding device.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following technical solutions：
One kind tissue freeze grinding device, including middle part have the grinding pot of grinding hole, and the grinding being adapted with grinding hole
Rod；The tank body of the grinding pot is double layer hollow structure, its hollow space forms the accommodating cavity of accommodating liquid nitrogen；The accommodating cavity it is upper
End is opened wide, and the upper end of accommodating cavity sets the lid of closing the upper end；There is the through hole passed through for grinding rod in the middle part of the lid.
Preferably, the grinding hole is cylindrical, its bottom is in cone shape.
Preferably, the grinding rod includes milling bar, is arranged at the grinding head of milling bar one end, and is arranged at grinding
The grinding handle of the bar other end.
Preferably, the lid at accommodating cavity overlap joint closing with being provided with sealing ring.
Preferably, the handle opened and closed easy to lid is set on the lid.
Compared with prior art, the invention has the advantages that：
（1）It is the configuration of the present invention is simple, easy to use, solve in tissue sample process of lapping, since liquid nitrogen volatilization causes to need
Otherwise stop adding the Operating Complexity of liquid nitrogen, maintain the low temperature environment of tissue sample in process of lapping, avoid tissue
In process of lapping liquid nitrogen or tissue splash caused by experimenter's frostbite may with tissue RNA degraded, so as to ensure that reality
The security and validity tested.
（2）After grinding and addition Trizol of the abrading tip mode that the present invention is set especially suitable for ultramicron sample
Continuous collection process, substantially increases the conventional efficient of trace sample.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is attached drawing needed in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, without creative efforts, can be with
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is the structure diagram of first embodiment of the invention.
Fig. 2 is the structure diagram of second embodiment of the invention.
Fig. 3 is the structure diagram of third embodiment of the invention.
Embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of not making the creative labor
Embodiment, belongs to the scope of protection of the invention.
Tissue freeze grinding device shown in Fig. 1, including middle part have grinding hole 1 grinding pot 2, and with grinding hole 1
The grinding rod 3 being adapted；The tank body of the grinding pot 2 is double layer hollow structure, its hollow space forms the accommodating of accommodating liquid nitrogen
Chamber 4；The open upper end of the accommodating cavity 4, and the upper end of accommodating cavity 4 sets the lid 5 of closing the upper end；The middle part of lid 5 tool
There is the through hole 6 passed through for grinding rod 3.
In the present embodiment, the grinding hole 1 is cylindrical, its bottom is in cone shape, can be right so in spin finishing
Micro-example plays good pulverization, while waste caused by liquid residue when reducing extraction.Obvious grinding hole 1 can also be adopted
With other applicable shapes.
Wherein, the grinding rod 3 includes milling bar 31, is arranged at the grinding head 32 of 31 one end of milling bar, and is arranged at
The grinding handle 33 of 31 other end of milling bar.Obvious milling bar 31 can also use other applicable structure types.
As a further improvement, the lid 5 overlaps with accommodating cavity 4 and is provided with sealing ring 7 at closing, to improve sealing
Property.
Tissue freeze grinding device shown in shown in Fig. 2, wherein setting the handle opened and closed easy to lid 5 on the lid 5
8, which is ring-shaped handle, it is clear that the handle that can also take other form.Remaining is essentially identical with the embodiment shown in Fig. 1.
Tissue freeze grinding device shown in shown in Fig. 3, wherein employ the grinding hole of another structure type, remaining with
Embodiment shown in Fig. 1 is essentially identical.
The accommodating cavity 4 of the present invention can pour into more liquid nitrogen, and liquid nitrogen can close liquid nitrogen region with lid 5 after pouring into,
There is sealing ring 7 between lid 5 and lapping device, it is possible to reduce liquid nitrogen volatilizees.4 inner wall of accommodating cavity uses and leads gentle and kindhearted good material,
Micro tissue to be ground can be put into grinding hole 1, rotatably ground with grinding rod 3, the bottom shape of grinding rod 3 and grinding
Hole 1 is agreed with, and 1 hole wall of grinding hole uses the rough surface of similar frosted in favor of grinding with the surface of grinding rod 3.End to be ground
Afterwards, appropriate Trizol is added, is suctioned out again after fully being mixed with the powder after grinding and moves into centrifuge tube progress next step experiment.If
Ultramicron tissue, can prepare with the sizeable centrifuge tube in 1 bottom of grinding hole, centrifuge tube is put into grinding hole 1, then by group
Knit and be put into centrifuge tube, then be ground with grinding rod 3, directly add Trizol after to be ground in centrifuge tube, mix
After directly take out centrifuge tube carry out next step experiment.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on, should all be included in the protection scope of the present invention god.
Claims (5)
1. one kind tissue freeze grinding device, including middle part have the grinding pot of grinding hole, and what is be adapted with grinding hole grind
Frotton；It is characterized in that：The tank body of the grinding pot is double layer hollow structure, its hollow space forms the accommodating of accommodating liquid nitrogen
Chamber；The open upper end of the accommodating cavity, and the upper end of accommodating cavity sets the lid of closing the upper end；Have in the middle part of the lid for grinding
The through hole that frotton passes through.
2. freeze grinding device is organized according to claim 1, it is characterised in that：The grinding hole is cylindrical, its bottom
In cone shape.
3. tissue freeze grinding device according to claim 1 or claim 2, it is characterised in that：The grinding rod includes milling bar, if
The grinding head of milling bar one end is placed in, and is arranged at the grinding handle of the milling bar other end.
4. tissue freeze grinding device according to claim 1 or claim 2, it is characterised in that：The lid and accommodating cavity lap jointss
The place of closing is provided with sealing ring.
5. tissue freeze grinding device according to claim 1 or claim 2, it is characterised in that：Set on the lid and be easy to lid
The handle of keying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810009041.5A CN107904153A (en) | 2018-01-04 | 2018-01-04 | One kind tissue freeze grinding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810009041.5A CN107904153A (en) | 2018-01-04 | 2018-01-04 | One kind tissue freeze grinding device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107904153A true CN107904153A (en) | 2018-04-13 |
Family
ID=61871079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810009041.5A Pending CN107904153A (en) | 2018-01-04 | 2018-01-04 | One kind tissue freeze grinding device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107904153A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110947488A (en) * | 2020-01-05 | 2020-04-03 | 孙晓琦 | Micro-tissue mortar and bracket |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4505433A (en) * | 1982-09-28 | 1985-03-19 | Selenke William M | Tissue grinding and transporting device |
| CN201200921Y (en) * | 2008-05-28 | 2009-03-04 | 梁晓南 | Freezing grinder |
| CN202057530U (en) * | 2011-03-21 | 2011-11-30 | 泸州医学院 | Micro grinder |
| CN203602620U (en) * | 2013-10-15 | 2014-05-21 | 中国农业大学 | Low-temperature grinding box for tissue cell nucleic acid extraction |
-
2018
- 2018-01-04 CN CN201810009041.5A patent/CN107904153A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4505433A (en) * | 1982-09-28 | 1985-03-19 | Selenke William M | Tissue grinding and transporting device |
| CN201200921Y (en) * | 2008-05-28 | 2009-03-04 | 梁晓南 | Freezing grinder |
| CN202057530U (en) * | 2011-03-21 | 2011-11-30 | 泸州医学院 | Micro grinder |
| CN203602620U (en) * | 2013-10-15 | 2014-05-21 | 中国农业大学 | Low-temperature grinding box for tissue cell nucleic acid extraction |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110947488A (en) * | 2020-01-05 | 2020-04-03 | 孙晓琦 | Micro-tissue mortar and bracket |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180413 |