CN207992247U - Full-automatic immunoblotting instrument - Google Patents
Full-automatic immunoblotting instrument Download PDFInfo
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- CN207992247U CN207992247U CN201820394734.6U CN201820394734U CN207992247U CN 207992247 U CN207992247 U CN 207992247U CN 201820394734 U CN201820394734 U CN 201820394734U CN 207992247 U CN207992247 U CN 207992247U
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Abstract
The utility model discloses a kind of full-automatic immunoblotting instrument, including body, moving track, film bar groove and sample rack are provided on body.Movement is provided with first movement arm on moving track；Film bar groove has multiple and arranged side by side, and film bar groove is for housing blotting membrane；Multiple sample tube grooves are offered on sample rack, sample tube groove is for placing sample tube.Sample-adding syringe needle is provided on first movement arm, sample-adding syringe needle moves for the sample liquid in sample drawn test tube and along first direction, sample liquid is added drop-wise on blotting membrane.Sample tube groove and film bar groove are correspondingly arranged in a first direction.Full-automatic immunoblotting instrument in present embodiment can place multiple blotting membranes in film bar groove, be carried out at the same time multiple project operations, substantially increase the degree of automation of immunoblot experiment, be truly realized efficient and unattended.Meanwhile film bar groove and test tube groove are correspondingly arranged, and are reduced the displacement distance of sample-adding syringe needle, are further improved operating efficiency.
Description
Technical field
The utility model is related to Western blotting detection field more particularly to a kind of full-automatic immunoblotting instrument.
Background technology
Clinical immunization Blot experiment is widely used in autoantibody detection, anaphylactogen detection, infectious disease confirmation etc..It is existing
In the structure of immunoblotting instrument, sample is located at the both ends of board slot, and single needle loading time is long, causes operating time length, step more, knot
Fruit preserves also inconvenient.In addition, can only at most be carried out at the same time the experiment of two projects on one piece of board slot, sample needle is also only once
Property feed head pattern, affects conventional efficient.
Utility model content
A kind of full-automatic immunoblotting instrument that the utility model embodiment provides, including body are arranged on the body
There are moving track, film bar groove and sample rack,
Movement is provided with first movement arm on the moving track；The film bar groove has multiple and arranged side by side, the film
Bar groove is for housing blotting membrane；Multiple sample tube grooves are offered on the sample rack, the sample tube groove is for putting
Set sample tube；
Sample-adding syringe needle is provided on the first movement arm, the sample-adding syringe needle is used to extract the sample in the sample tube
This liquid is simultaneously moved along first direction, and the sample liquid is added drop-wise on the blotting membrane,
The sample tube groove and the film bar groove are correspondingly arranged in said first direction.
Full-automatic immunoblotting instrument in the utility model embodiment can place multiple blotting membranes in film bar groove, simultaneously
Multiple project operations are carried out, the degree of automation of immunoblot experiment is substantially increased, are truly realized efficient and unattended.
Meanwhile film bar groove and test tube groove are correspondingly arranged, and are reduced the displacement distance of sample-adding syringe needle, are further improved operating efficiency.
In some embodiments, the film bar groove has 50, and the sample tube groove has 50.
In some embodiments, the sample tube groove is in double setting, and the sample tube of first row is recessed
The sample tube groove stagger setting of slot and second row.
In some embodiments, interpretation structure is additionally provided on the first movement arm, the interpretation structure includes electricity
Lotus coupling device photographic system.
In some embodiments, the long side of the length and the film bar groove of the interpretation structure in said first direction
The equal length in direction.
In some embodiments, length of the interpretation structure on the direction vertical with the first direction be more than etc.
In the length of the short side direction of the film bar groove.
In some embodiments, it is additionally provided with the second moving arm on the moving track, is set on second moving arm
Multiple suction waste fluid channels and multiple reagent adding channels are equipped with, the waste fluid channel of inhaling is arranged along the orientation of the film bar groove,
The reagent adding channel is arranged along the long side direction of the film bar groove.
In some embodiments, the suction waste fluid channel has 4, and the reagent adding channel has 10.
In some embodiments, the height of the sample rack is less than the height of the top surface of the film bar groove, the sample
After test tube is placed on the sample rack, the top of the sample tube is less than plane where the film bar groove.
In some embodiments, the sample-adding syringe needle is replaceable.
The additional aspect and advantage of the utility model embodiment will be set forth in part in the description, partly will be under
Become apparent in the description in face, or is recognized by the practice of the utility model.
Description of the drawings
In description of the above-mentioned and/or additional aspect and advantage of the utility model from combination following accompanying drawings to embodiment
It will be apparent and be readily appreciated that, wherein：
Fig. 1 is the dimensional structure diagram of the full-automatic immunoblotting instrument of the utility model embodiment；
Fig. 2 is another dimensional structure diagram of the full-automatic immunoblotting instrument of the utility model embodiment；
Fig. 3 is the dimensional structure diagram of the film bar groove of the full-automatic immunoblotting instrument of the utility model embodiment；
Fig. 4 is the stereochemical structure signal of the second moving arm of the full-automatic immunoblotting instrument of the utility model embodiment
Figure；
Fig. 5 is that the stereochemical structure of another second moving arm of the full-automatic immunoblotting instrument of the utility model embodiment is shown
It is intended to；
Fig. 6 is the stereochemical structure signal of the first movement arm of the full-automatic immunoblotting instrument of the utility model embodiment
Figure；
Fig. 7 is the dimensional structure diagram of the interpretation structure of the full-automatic immunoblotting instrument of the utility model embodiment.
Specific implementation mode
The embodiment of the utility model is described below in detail, the example of the embodiment is shown in the accompanying drawings, wherein
Same or similar label indicates same or similar element or element with the same or similar functions from beginning to end.Lead to below
It crosses the embodiment being described with reference to the drawings to be exemplary, is only used for explaining the utility model, and should not be understood as to this practicality
Novel limitation.
In the description of the present invention, it should be understood that term "center", " longitudinal direction ", " transverse direction ", " length ", " width
Degree ", " thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside",
The orientation or positional relationship of the instructions such as " clockwise ", " counterclockwise " be based on the orientation or positional relationship shown in the drawings, be only for
Described convenient for description the utility model and simplifying, do not indicate or imply the indicated device or element must have it is specific
Orientation, with specific azimuth configuration and operation, therefore should not be understood as limiting the present invention.In addition, term " first ",
" second " is used for description purposes only, and is not understood to indicate or imply relative importance or implicitly indicates indicated technology
The quantity of feature.Define " first " as a result, the feature of " second " can explicitly or implicitly include it is one or more
The feature.The meaning of " plurality " is two or more in the description of the present invention, unless otherwise clearly specific
It limits.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, can also be to be electrically connected or can mutually communicate；It can be directly connected, it can also be in
Between medium be indirectly connected, can be the interaction relationship of the connection or two elements inside two elements.For this field
For those of ordinary skill, the concrete meaning of above-mentioned term in the present invention can be understood as the case may be.
In the description of the present invention, unless otherwise clearly defined and limited, fisrt feature second feature it
"upper" or "lower" may include that the first and second features are in direct contact, can also include the first and second features not be directly to connect
It touches but passes through the other characterisation contact between them.Moreover, fisrt feature second feature " on ", " top " and " on
Face " includes fisrt feature right over second feature and oblique upper, or to be merely representative of fisrt feature level height special higher than second
Sign.Fisrt feature second feature " under ", " lower section " and " below " include fisrt feature immediately below second feature and obliquely downward
Side, or be merely representative of fisrt feature level height and be less than second feature.
Following disclosure provides many different embodiments or example is used for realizing the different structure of the utility model.
In order to simplify the disclosure of the utility model, hereinafter the component of specific examples and setting are described.Certainly, they are only
Example, and purpose does not lie in limitation the utility model.In addition, the utility model can in different examples repeat reference numerals
And/or reference letter, this repetition are for purposes of simplicity and clarity, itself not indicate discussed various embodiments
And/or the relationship between setting.In addition, the example for the various specific techniques and material that the utility model provides, but this
Field those of ordinary skill can be appreciated that the application of other techniques and/or the use of other materials.
Please refer to Fig.1-Fig. 7, a kind of full-automatic immunoblotting instrument 100 that the utility model embodiment provides, including machine
Body 10 is provided with moving track 12, film bar groove 14 and sample rack 16 on body 10.Movement is provided with the first shifting on moving track 12
Swing arm 122；Film bar groove 14 has multiple and arranged side by side, and film bar groove 14 is for housing blotting membrane；It is offered on sample rack 16 multiple
Sample tube groove 162, sample tube groove 162 is for placing sample tube 164.Sample-adding is provided on first movement arm 122
Syringe needle 124, sample-adding syringe needle 124 is moved for the sample liquid in sample drawn test tube 164 and along first direction X, by sample liquid
Body is added drop-wise on blotting membrane.Sample tube groove 162 and film bar groove 14 are correspondingly arranged on X in a first direction.
Full-automatic immunoblotting instrument 100 in the utility model embodiment can place multiple blotting membranes in film bar groove 14,
Be carried out at the same time multiple project operations, substantially increase the degree of automation of immunoblot experiment, be truly realized it is efficient and nobody
It is on duty.Meanwhile film bar groove 14 and test tube groove are correspondingly arranged, and are reduced the displacement distance of sample-adding syringe needle 124, are further improved
Operating efficiency.
In addition, the film bar groove 14 and sample rack 16 that are arranged in a one-to-one correspondence, reduce the back and forth movement row of sample-adding syringe needle 124
Journey substantially reduces loading time.
Specifically, the full-automatic immunoblotting instrument 100 in the utility model embodiment is as the auxiliary of immunoblot experiment
Assistant engineer has, be mainly used for immunoblot experiment automatic sample, liquid feeding, concussion incubations, reagent adding, cleaning, air-dried, result are taken pictures and
The operations such as area of computer aided interpretation.
Immunoblotting is also known as Western blotting, is specifically binding come certain in detection of complex sample according to antigen-antibody
The method of kind albumen, high specific and sensibility with high resolution and solid-phase immunoassay.Immune-blotting method method is wet
Immobilization matrix film is flexible, easily operated；The large biological molecule of immobilization can be equably close with various immunological probes, will not picture
Gel is obstructed by aperture like that；Immunoblotting assay only needs a small amount of reagent, and resource is greatly saved；Multiple copy can be made simultaneously
Shellfish is used for a variety of analyses and identification.
The flow of immune-blotting method approximately as：
After protein to be detected is transferred on blotting membrane, blotting membrane is closed, then incubated with Primary antibodies, cleaning, then
Antibody incubation, cleaning are connect with two level again, is detected according to colorimetric method or chemiluminescence method, interpretation of result.
Specifically, primary antibody is the antibody of testing protein specificity, and secondary antibody is marked radioactive isotope or is combined with
Fluorescent material, gold particle, or coupled with enzymes such as-HRP or-AP.Typically now use includes radioactive labels autography technology, ratio
The secondary antibody of the substrates and combination-HRP or-AP such as color detection, chemiluminescence, bioluminescence, chemiluminescence, fluorescence and Immuno gold detection
It incubates.In this system, after substrate is added, its generation colored precipitate (colorimetric detection) of enzymatic or chemiluminescence, fluorescence etc.
Then optical signal uses film or CCD, Fluorescence Scanner to carry out signal collection.
Testing protein generates colourful signal after being combined with specific antibody.The signal of colorimetric detection is colored precipitate substance.
What CL and BL detected is the optical signal that reaction itself generates.Difference lies in substrate source differences for the two.Change
The enzymatic preparation learned in fluorescence detection method can send out fluorescence signal.The signal of fluoroscopic examination, autoradiograph, Immuno gold detection
All it is that labeled secondary antibody generates.The secondary antibody of fluoroscopic examination is marked with fluorophor；The secondary antibody mark of autoradiography
There is radioactive isotope；The secondary antibody of Immuno gold detection indicates gold, and signal can be precipitated by silver and be strengthened.
In some embodiments, film bar groove 14 has 50, and sample tube groove 162 has 50.
In this way, the full-automatic immunoblotting instrument 100 in present embodiment can be carried out at the same time more projects, albumen is improved
The efficiency of quality detection.
Specifically, the full-automatic immunoblotting instrument 100 in present embodiment includes 10 peristaltic pumps and 10 fluid passages,
To ensure that the efficiency of protein detection.In some embodiments, sample tube groove 162 is in double setting, and first
The sample tube groove 162 of row and 162 stagger setting of sample tube groove of second row.
In this way, when sample-adding syringe needle 124 moves between double sample tube 164, it will not accidentally encounter sample tube 164, subtract
The error for having lacked immune-blotting method experiment, improves the efficiency of test.
In some embodiments, interpretation structure 126 is additionally provided on first movement arm 122, interpretation structure 126 includes electricity
128 photographic system of lotus coupling device (CCD).
In this way, for relatively various chemiluminescence detection technologies, CCD imagings have be easiest to, be most accurate, fireballing spy
Point.CCD camera has the very wide range of linearity, different according to the pixel depth of system, and the range of linearity is from 2.0-4.8 etc..
That is even if signal strength variation reaches tens million of to tens thousand of times, still can accurately measure.CCD systems can be experiment
Record, analysis, the shared and storage of data are brought convenience, and without buying the various consumptions needed for film development regularly
Material.Another advantage of CCD is to reach the sensitivity technique limit in the relatively short time.
In some embodiments, the long side direction of the length on X and film bar groove 14 in a first direction of interpretation structure 126
Equal length.
In this way, when judging the blotting membrane in structural scan film bar groove 14, the face for judging structure cover film bar groove 14 is increased
Product so that interpretation structure 126 move it is primary after can interpretation to more blotting membranes, improve interpretation efficiency, also improve and sweep
Retouch the accuracy of interpretation.
In some embodiments, length of the interpretation structure 126 on the direction vertical with first direction X is more than or equal to film
The length of the short side direction of bar groove 14.
In this way, interpretation structure 126 move it is primary after can simultaneously in interpretation more multimembrane bar groove 14 blotting membrane, reduce first
The movement of moving arm 122 improves the efficiency of interpretation.
In some embodiments, it is additionally provided with the second moving arm 121 on moving track 12, is set on the second moving arm 121
It is equipped with multiple suction waste fluid channels 123 and multiple reagent adding channels 125, waste fluid channel 123 is inhaled and is set along the orientation of film bar groove 14
It sets, reagent adding channel 125 is arranged along the long side direction of film bar groove 14.
Specifically, inhaling waste fluid channel 123 can move in the vertical direction, to ensure to inhale waste fluid channel 123 by film bar groove 14
Interior waste liquid absorbs completely, avoids omitting, be had an impact to subsequent operation.
The orientation for inhaling waste fluid channel 123 along film bar groove 14 is arranged, and can handle the waste liquid in multiple film bar grooves 14 simultaneously,
Improve waste liquid infiltration rate.Reagent adding channel 125 is arranged along the long side direction of film bar groove 14, ensure that test solution can be uniformly added into
Into film bar groove 14, convenient in subsequent detection, detection liquid energy and test fluid to be checked uniformly contact, and ensure the reliable of testing result
Property.
In some embodiments, inhaling waste fluid channel 123 has 4, and reagent adding channel 125 has 10.
In this way, the time that waste liquid is drawn in 4 imbibition channels greatly shortens.Similarly, the time for adding reagent also greatly shortens.
In addition, 4 are inhaled waste fluid channels 123 and ensure that the experiment condition of each film bar groove 14 is consistent, from ensuring the accurate of experimental result
Property.
In some embodiments, the height of sample rack 16 is less than the height of the top surface of film bar groove 14, and sample tube 164 is put
After setting on sample rack 16, the top of sample tube 164 is less than 14 place plane of film bar groove.
In this way, when sample-adding syringe needle 124 moves between film bar groove 14 and sample rack 16, sample tube 164 will not be touched,
It avoids polluting sample tube 164, sample-adding syringe needle 124 on the move is also avoided to damage sample tube 164.
In some embodiments, sample-adding syringe needle 124 is replaceable.
In this way, sample-adding syringe needle 124 can be needed replacing according to experiment to be disposably loaded syringe needle 124 or being permanently loaded syringe needle
124, achieve the purpose that save resource and ensures working specification.
In the present embodiment, operator edits the program of experimental project on the control device in advance, before experiment starts
Bar code on scanned samples test tube 164, and sample tube 164 is placed sequentially on sample rack 16, trace item is placed on film
In bar groove 14；After starting instrument, it is automatically performed whole experiment process；Finally taken pictures by CCD, area of computer aided interpretation experiment knot
Fruit, generates and print result is reported.
In the full-automatic immunoblotting instrument 100 of present embodiment, air-dried unit 127 is additionally provided on the second moving arm 121.
Air-dry the rate of drying that unit 127 accelerates film bar groove 14.
In the description of this specification, reference term " embodiment ", " certain embodiments ", " schematically implementation
What the description of mode ", " example ", " specific example " or " some examples " etc. meant to describe in conjunction with the embodiment or example
Particular features, structures, materials, or characteristics are contained at least one embodiment or example of the utility model.In this explanation
In book, schematic expression of the above terms are not necessarily referring to identical embodiment or example.Moreover, the specific spy of description
Sign, structure, material or feature can be combined in any suitable manner in any one or more embodiments or example.
While there has been shown and described that the embodiment of the utility model, those skilled in the art can manage
Solution：A variety of to the progress of these embodiments can change in the case where not departing from the principles of the present invention and objective, change,
It replaces and modification, the scope of the utility model is limited by claim and its equivalent.
Claims (10)
1. a kind of full-automatic immunoblotting instrument, which is characterized in that including body, moving track, film item are provided on the body
Slot and sample rack,
Movement is provided with first movement arm on the moving track；The film bar groove has multiple and arranged side by side, the film bar groove
For housing blotting membrane；Multiple sample tube grooves are offered on the sample rack, the sample tube groove is for placing sample
This test tube；
Sample-adding syringe needle is provided on the first movement arm, the sample-adding syringe needle is used to extract the sample liquid in the sample tube
Body is simultaneously moved along first direction, and the sample liquid is added drop-wise on the blotting membrane,
The sample tube groove and the film bar groove are correspondingly arranged in said first direction.
2. full-automatic immunoblotting instrument according to claim 1, which is characterized in that the film bar groove has 50, the sample
This test tube groove has 50.
3. full-automatic immunoblotting instrument according to claim 2, which is characterized in that the sample tube groove is set in double
It sets, and the sample tube groove stagger setting of the sample tube groove of first row and second row.
4. full-automatic immunoblotting instrument according to claim 1, which is characterized in that be additionally provided on the first movement arm
Interpretation structure, the interpretation structure include charge coupled device photographic system.
5. full-automatic immunoblotting instrument according to claim 4, which is characterized in that the interpretation structure is in the first party
The equal length of the long side direction of upward length and the film bar groove.
6. full-automatic immunoblotting instrument according to claim 4, which is characterized in that the interpretation structure is with described first
Length on the vertical direction in direction is more than or equal to the length of the short side direction of the film bar groove.
7. full-automatic immunoblotting instrument according to claim 1, which is characterized in that be additionally provided on the moving track
Two moving arms are provided with multiple suction waste fluid channels and multiple reagent adding channels, the suction waste fluid channel on second moving arm
Orientation along the film bar groove is arranged, and the reagent adding channel is arranged along the long side direction of the film bar groove.
8. full-automatic immunoblotting instrument according to claim 7, which is characterized in that the suction waste fluid channel has 4, described
Reagent adding channel has 10.
9. full-automatic immunoblotting instrument according to claim 1, which is characterized in that the height of the sample rack is less than described
The height of the top surface of film bar groove, after the sample tube is placed on the sample rack, the top of the sample tube is less than institute
Plane where stating film bar groove.
10. full-automatic immunoblotting instrument according to claim 1, which is characterized in that the sample-adding syringe needle is replaceable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820394734.6U CN207992247U (en) | 2018-03-22 | 2018-03-22 | Full-automatic immunoblotting instrument |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820394734.6U CN207992247U (en) | 2018-03-22 | 2018-03-22 | Full-automatic immunoblotting instrument |
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| CN207992247U true CN207992247U (en) | 2018-10-19 |
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| CN201820394734.6U Active CN207992247U (en) | 2018-03-22 | 2018-03-22 | Full-automatic immunoblotting instrument |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109900912A (en) * | 2019-03-19 | 2019-06-18 | 莫纳（苏州）生物科技有限公司 | A kind of clamping type immunoblotting assay instrument and its control system |
| CN111856050A (en) * | 2020-07-04 | 2020-10-30 | 欧亚先锋生物技术投资(北京)有限责任公司 | Full-automatic immunoblotting instrument and detection method |
-
2018
- 2018-03-22 CN CN201820394734.6U patent/CN207992247U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109900912A (en) * | 2019-03-19 | 2019-06-18 | 莫纳（苏州）生物科技有限公司 | A kind of clamping type immunoblotting assay instrument and its control system |
| CN111856050A (en) * | 2020-07-04 | 2020-10-30 | 欧亚先锋生物技术投资(北京)有限责任公司 | Full-automatic immunoblotting instrument and detection method |
| CN111856050B (en) * | 2020-07-04 | 2024-03-29 | 欧亚先锋生物技术投资(北京)有限责任公司 | Full-automatic immunoblotter and detection method |
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