CN207978739U - A kind of shading sleeve and the root-knot nematode tissue culture bottle for being cased with shading sleeve - Google Patents

A kind of shading sleeve and the root-knot nematode tissue culture bottle for being cased with shading sleeve Download PDF

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Publication number
CN207978739U
CN207978739U CN201820081869.7U CN201820081869U CN207978739U CN 207978739 U CN207978739 U CN 207978739U CN 201820081869 U CN201820081869 U CN 201820081869U CN 207978739 U CN207978739 U CN 207978739U
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China
Prior art keywords
root
shading sleeve
bottle
knot nematode
tissue culture
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CN201820081869.7U
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赵文超
魏婧薇
王绍辉
张旋
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Beijing University of Agriculture
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Beijing University of Agriculture
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Abstract

The utility model is related to a kind of shading sleeves and the root-knot nematode tissue culture bottle for being cased with shading sleeve, wherein shading sleeve it is characterized in that:Including bottom plate and the cylindrical sidewall in the bottom edge is set, the bottom plate and the cylindrical sidewall are that opaque material is made;It is cased with the root-knot nematode tissue culture bottle of shading sleeve, it is characterised in that:It includes the bottle of top end opening and covers the lid closed in the bottle open end, and the shading sleeve is arranged in the bottom of the bottle.The utility model shading sleeve includes bottom plate and the cylindrical sidewall in bottom edge is arranged, shading sleeve and cylindrical sidewall are made of opaque material, the bottom that shading sleeve is sleeved on tissue culture bottle makes the culture medium in tissue culture bottle avoid the direct projection of light, the natural animation for building root-knot nematode, is more advantageous to the growth and development of root-knot nematode.

Description

A kind of shading sleeve and the root-knot nematode tissue culture bottle for being cased with shading sleeve
Technical field
The utility model is related to a kind of shading sleeves and the root-knot nematode tissue culture bottle for being cased with shading sleeve, belong to laboratory paraphernalia neck Domain.
Background technology
Root-knot nematode (Meloidogyne spp) is distributed widely in all over the world, it is that one kind is very important, endangers pole Big plant pathogeny line insect.More than 30 kinds of Vegetables can be endangered, and again with the larger tomato of cultivated area, cucumber, celery etc. It is aggrieved most heavy.The whole world every year due to root-knot nematode harm caused by agricultural product loss more than 100,000,000,000 dollars, it is Chinese only To the damage losses of various vegetables just up to 3,000,000,000 dollars or more, the economic loss of 10%-15%, serious loss is caused to reach every year To 30%-40%, or even total crop failure.
Root knot nematode disease main harm vegetables root, lateral root and fibrous root are most vulnerable.Aggrieved root generates of different sizes Root knot, the knob that mostly spherical or cone differs in size start as milky, and the later stage becomes filbert, and surface has sometimes Cracking.Vegetables are infected that rear growth and development is slow, and blade is small and yellow by root-knot nematode.When morbidity weight, plant is short and small, shaky or knot It is real bad, when meeting high temperature and drought weather, the easily withered necrosis of plant.There is generation overlap phenomenon in root-knot nematode fast growing, and It is difficult to thoroughly remove, traditional control method is very limited in the upper effect of containment root-knot nematode generation, it is difficult to effect a radical cure, gives peasant households The economic loss brought is extremely serious.
Root nematode disease in order to prevent, carry out root-knot nematode correlative study work be very it is necessary to.Traditional research Root-knot nematode and the mode of host's interaction are soil cultivations, but the shortcomings that this mode is can not to observe directly nematode in soil In active procedure, moreover, obtain host plant root be difficult to clean thoroughly, for it is subsequent test bring inconvenience.
Root-knot nematode is inoculated in the aseptic seedling grown on the transparent culture medium in tissue culture bottle, this mode can overcome with Upper disadvantage.This method takes up space small, and the root-knot nematode of culture is clean, convenient for observation control, is provided for subsequent research work It is convenient.However, the Natural Survival space of root-knot nematode is the dark space inside soil, light penetrates culture medium direct projection root knot line Worm can inevitably impact the physiological activity of root-knot nematode, to make root-knot nematode result of study generate certain deviation.
Invention content
In view of the above-mentioned problems, the purpose of this utility model is to provide a kind of shading sleeve and can simulate root-knot nematode growing environment The root-knot nematode tissue culture bottle for being cased with shading sleeve.
To achieve the above object, the utility model uses following technical scheme:A kind of shading sleeve, it is characterised in that:Including Bottom plate and the cylindrical sidewall in the bottom edge is set, the bottom plate and the cylindrical sidewall are opaque material system At.
The through-hole being discharged conducive to air when being provided on the bottom plate for installing tissue culture bottle.
The outer surface arc transition of the outer bottom of the bottom plate and the cylindrical sidewall.
A kind of root-knot nematode tissue culture bottle being cased with shading sleeve, it is characterised in that:It includes bottle and the lid conjunction of top end opening Lid in the bottle open end is arranged the shading sleeve in the bottom of the bottle.
The height of the shading sleeve is the 1/5 of the bottle height.
The utility model has the following advantages due to taking above technical scheme:1, the utility model shading sleeve includes It bottom plate and the cylindrical sidewall in bottom edge, shading sleeve and cylindrical sidewall is set is made of opaque material, shading sleeve is set with Culture medium in the bottom of tissue culture bottle makes tissue culture bottle avoids the direct projection of light, builds the natural life shape of root-knot nematode State is more advantageous to the growth and development of root-knot nematode.2, the utility model can reuse, and economize on resources, and install fast It is fast, convenient.
Description of the drawings
Fig. 1 is the vertical view of the utility model;
Fig. 2 is the axonometric drawing of the utility model;
Fig. 3 is the structural schematic diagram that the utility model is sleeved on to tissue culture bottle bottom.
Specific implementation mode
The utility model is described in detail with reference to the accompanying drawings and examples.
As shown in Fig. 1~2, the utility model proposes a kind of shading sleeve, which includes bottom plate 1 and is arranged in bottom plate The cylindrical sidewall 2 at 1 edge, bottom plate 1 and cylindrical sidewall 2 are that opaque material is made.
In above-described embodiment, the through-hole 3 being discharged conducive to air when being provided on bottom plate 1 for installing tissue culture bottle, through-hole 3 Setting so that bottom plate 1 is adjacent to the bottom end of tissue culture bottle.
In above-described embodiment, the outer bottom of bottom plate 1 and the outer surface arc transition of cylindrical sidewall 2.
As shown in figure 3, the utility model also proposed a kind of root-knot nematode tissue culture bottle being cased with shading sleeve, including top is opened The bottle 4 and lid of mouth close the lid 5 in 4 open end of bottle, and shading sleeve is arranged in the bottom of bottle 4.
In above-described embodiment, the height of shading sleeve is the 1/5 of 4 height of bottle, shading sleeve energy behind the bottom of tissue culture bottle Transparent culture medium is effectively covered, avoids strong light to the direct projection of culture medium, has built the natural animation of root-knot nematode.Group The part that training bottle upper end is not blocked by shading sleeve is conducive to observe plant root and its neighbouring root-knot nematode in culture medium at any time and lives Dynamic state.
The utility model is only illustrated with above-described embodiment, and structure, installation position and its connection of each component all have Changed, it is all that individual part is changed according to the utility model principle on the basis of technical solutions of the utility model Into and equivalents, should not exclude except the scope of protection of the utility model.

Claims (3)

1. a kind of shading sleeve, it is characterised in that:Including bottom plate and the cylindrical sidewall in the bottom edge is set, the bottom plate and The cylindrical sidewall is that opaque material is made;It is conducive to air when being provided with for installing tissue culture bottle on the bottom plate to arrange The through-hole gone out, the outer surface arc transition of the outer bottom of the bottom plate and the cylindrical sidewall.
2. a kind of root-knot nematode tissue culture bottle being cased with shading sleeve as described in claim 1, it is characterised in that:It includes top end opening Bottle and lid close the bottle open end lid, be arranged the shading sleeve in the bottom of the bottle.
3. a kind of root-knot nematode tissue culture bottle as claimed in claim 2, it is characterised in that:The height of the shading sleeve is the bottle The 1/5 of body height.
CN201820081869.7U 2018-01-18 2018-01-18 A kind of shading sleeve and the root-knot nematode tissue culture bottle for being cased with shading sleeve Active CN207978739U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820081869.7U CN207978739U (en) 2018-01-18 2018-01-18 A kind of shading sleeve and the root-knot nematode tissue culture bottle for being cased with shading sleeve

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820081869.7U CN207978739U (en) 2018-01-18 2018-01-18 A kind of shading sleeve and the root-knot nematode tissue culture bottle for being cased with shading sleeve

Publications (1)

Publication Number Publication Date
CN207978739U true CN207978739U (en) 2018-10-19

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201820081869.7U Active CN207978739U (en) 2018-01-18 2018-01-18 A kind of shading sleeve and the root-knot nematode tissue culture bottle for being cased with shading sleeve

Country Status (1)

Country Link
CN (1) CN207978739U (en)

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