CN207937357U - A kind of Streptavidin spr sensor based on fiber bragg grating - Google Patents
A kind of Streptavidin spr sensor based on fiber bragg grating Download PDFInfo
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- CN207937357U CN207937357U CN201820496307.9U CN201820496307U CN207937357U CN 207937357 U CN207937357 U CN 207937357U CN 201820496307 U CN201820496307 U CN 201820496307U CN 207937357 U CN207937357 U CN 207937357U
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- bragg grating
- fiber bragg
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- streptavidin
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Abstract
A kind of Streptavidin spr sensor based on fiber bragg grating, including wideband light source, optoisolator, Polarization Controller, 2X1 couplers, SPR sensorgram probe, spectrometer based on fiber bragg grating, it is above-mentioned based on fiber bragg grating SPR sensorgram probe by multimode fibre, fiber bragg grating, total reflection film and be coated with golden film, silane coupling agent, biotin film sensing unit form, when solution of streptavidin concentration changes, the effective refractive index on golden film surface changes, and causes the drift of SPR resonance wavelengths；When the temperature varies, the effective refractive index and screen periods of bragg grating fibre core can change, it shifts so as to cause Bragg reflection wavelengths, by spectrometer to the demodulation of SPR resonance wavelengths and Bragg reflection wavelength drift values, it can be achieved that solution of streptavidin and temperature biparameter while is detected.The utility model has many advantages, such as that simple in structure, measurement sensitivity is high, can be with real time on-line monitoring.
Description
Technical field
The utility model belongs to fiber-optic biosensor technical field, and in particular to a kind of chain based on fiber bragg grating
Mould Avidin spr sensor, using surface plasma wave to the sensitivity characteristic and biotin of external environment (refractive index) to chain
The mechanism of mould Avidin specific adsorption realizes the detection of various concentration Streptavidin.
Background technology
Surface plasma body resonant vibration (Surface Plasmon Resonance, abbreviation SPR) refers to the P when light source is sent out
When type polarizes polychromatic light by the interface of film metal and medium, if meeting incidence angle is more than the cirtical angle of total reflection, on surface
On form electron concentration gradient distribution, formed plasma oscillation, formed surface plasma wave, due to surface evanescent wave
The phenomenon that presence of field and metal complex refractivity index, makes the light for meeting resonance wavelength be partly absorbed, and the light of commplementary wave length is reflected.
Nineteen sixty-eight, roentgen Otto and Kretschmann respectively use (Attenuated Total Reflection, abbreviation
ATR method) realizes the excitation of the surface plasma of optical frequency wave band in an experiment.
Fiber bragg grating (fiber Bragg gratings, FBGs) is by carrying out permanent week in fiber core
Phase property index modulation is made, and index modulation direction is perpendicular to optical fiber axial direction.According to the fiber coupling theory of modules, work as incident light
When being propagated in fibre core, Mode Coupling is generated, the light wave of specific wavelength is reflected.1993, K.O.Hill et al. was covered using phase
Template writes fiber bragg grating processed, makes it possible the batch production of grating, has greatly pushed fiber bragg grating
Development.It is tunable to its all band, therefore optical fiber by the period and effective refractive index, the bragg wavelength of reflection that change grating
Bragg grating has been used in many sensory fields.1996, wavelength-division multiplex FBG was applied and is passed in temperature by Y.J.Rao et al.
Sensing system.2000, fiber bragg grating was written in M.J.Gander et al. in multi-core optical fiber, realized the survey of amount of bow
Amount.2006, Chen Jianjun et al. measured flow velocity using a kind of high-sensitivity optical fiber grating sensor, and experiment shows this
Sensor can sense the flow velocity size of at least 0.3mm/s.Ma in 2013 et al. is by one section of thin-core fibers and optical fiber Bragg light
Grid welding, the sensing arrangement can effectively excite cladding mode, to realize highly sensitive refractometry.The same year, Rong et al.
Fiber bragg grating is inscribed simultaneously in the fibre core and covering of thin-core fibers using femto-second laser, is carried out to being bent, vibrating
It measures.FBG has small, high sensitivity, a good characteristic anti-interference, at low cost etc., thus temperature, bending, refractive index,
The fields such as flow control are quickly grown, and also have tremendous potential to await development.
Streptavidin (Streptavidin, SA) is a kind of protein secreted in avidin streptomycete growth course,
The tetramer being made of four identical peptide chains, it has very strong suction-operated with biotin (Biotin), and a SA molecule can
To combine four biotin molecules.The affinity of biotin and Streptavidin is especially strong, and the suction-operated of the two is a kind of
With Streptavidin specific adsorption can only occur for the selective absorption of specificity, i.e. biotin, not occur with other protein
Specific adsorption, the two are extremely stablized once combined.SA can be coupled a series of macromolecular bioactive substances with biotin,
Multistage amplification can be generated by labels such as radioactive isotope, enzyme, fluoresceins again.However, in the detection side of Streptavidin
In method, radio-immunoassay radioactive element has radiativity to human body；Enzyme-linked immunosorbent assay program is cumbersome, takes
It is long；The shortcomings that spectrophotometer detection method is easy to be interfered by environmental factor, of high cost, and required sample is more, these methods big day of one's doom
Application of the Streptavidin in immunology is made.
For above-mentioned detection method device complexity, sample requirements are big, and program is cumbersome, and precision is not high and easily by environment temperature
The problems such as degree influences, the utility model proposes a kind of the Streptavidin spr sensor based on fiber bragg grating, structure letter
Single, easy to operate, small, precision is high, it can be achieved that Streptavidin concentration and temperature are monitored on-line simultaneously, has very strong practicality
Value.
Utility model content
In order to overcome above-mentioned detection method device complicated, sample requirements are big, and program is cumbersome, and precision is not high and Yi Shouhuan
The problems such as border temperature influences, it is easy to operate the utility model proposes a kind of simple in structure, it is small, precision it is high, it can be achieved that temperature and
Streptavidin concentration is monitored on-line simultaneously, the Streptavidin based on fiber bragg grating with very strong practical value
Spr sensor.
The utility model is the technical solution adopted for solving the technical problem：
A kind of Streptavidin spr sensor based on fiber bragg grating, including：Wideband light source, optoisolator, partially
Shake controller, 2X1 couplers, SPR sensorgram probe, spectrometer based on fiber bragg grating.
The output end of wideband light source is connected with the input terminal of optoisolator, the output end of optoisolator and Polarization Controller
Input terminal is connected, and one end of 2X1 coupler two-ports is connected with Polarization Controller, another termination spectrometer, 2X1 couplers one end
One end of mouth is connected with the SPR sensorgram probe based on fiber bragg grating；SPR sensorgram probe based on fiber bragg grating
By multimode fibre, fiber bragg grating, total reflection film and it is coated with golden film, the sensing district's groups of silane coupling agent, biotin film
At；125 μm of multimode fibre cladding diameter, 62.5 μm of fiber bragg grating core diameter are surplus after hydrofluoric acid solution corrodes
Remaining cladding thickness ensures that basic mode is coupled to SPR sensorgram area between 62.5nm-82.5nm；The thickness of golden film is in 30nm to 50nm
Between, the root mean square of surface roughness is less than or equal to 3nm；Total reflection film is to the reflectivity of resonance wavelength 90% or more；In gold
Film surface modifies silane coupling agent to fix biotin film, and biotin film has specific adsorption ability to Streptavidin.
The beneficial effects of the utility model are：
The utility model is using multimode fibre, bragg grating, total reflection film and is coated with golden film, silane coupled
Agent, biotin film sensing unit constitute SPR sensorgram head, and fix biotin film in golden film surface modification silane coupling agent, it is raw
Object element film has specific adsorption ability, when the concentration of solution of streptavidin changes, golden film table to Streptavidin
Significant change occurs for the refractive index in face, and SPR resonance wavelengths generate drift；When the temperature varies, bragg grating is fine
The effective refractive index and screen periods of core can change, and shift so as to cause Bragg reflection wavelengths, this realizes strepto-
Discriminating measurement while Avidin and temperature biparameter, substantially increases detection sensitivity；
Sensing head used in the utility model is sonde-type shape, is applicable to complex detection environment, uses more
For convenience；
The sensing head of the utility model can carry out different material special by changing the modification mode outside its golden film surface
Anisotropic selective absorption, while bragg grating structure can realize the measurement to temperature, to realize multi-component (especially
Thermally sensitive substance) identification, enrich the application of this sensor；
This total reflection film for testing novel sensing probe end makes incident light increase by one in the optical path difference of sensor internal
Times, resonance effect doubles, and improves sensitivity of the SPR resonance wavelengths to extraneous variations in refractive index.
Description of the drawings
Fig. 1 is the device and structural schematic diagram of the Streptavidin spr sensor based on fiber bragg grating.
Specific implementation mode
Utility model is further described below in conjunction with the accompanying drawings.
As shown in Figure 1, the Streptavidin spr sensor based on Bragg grating, including：Wideband light source 1, optoisolator
2, Polarization Controller 3,2X1 couplers 4, SPR sensorgram probe 5, spectrometer 12 based on fiber bragg grating；
The working method of the utility model is：Wideband light source 1 sends out signal light, and optoisolator 2 is input to by Transmission Fibers,
The optical signal that optoisolator 2 exports becomes P polarization light (P polarization light can realize surface etc. by the control output of Polarization Controller 3
The excitation of ion bulk wave (SPW)), P polarization light is inputted from one end of 4 two-port of 2X1 couplers, from 4 Single port of 2X1 couplers
One end exports, and enters the probe of the SPR sensorgram based on fiber bragg grating 5 via Transmission Fibers, is based on optical fiber Bragg light
The SPR sensorgram probe 5 of grid by multimode fibre 11, fiber bragg grating 6, total reflection film 10 and is coated with golden film 7, silane coupled
Agent 8, biotin film 9 sensing unit composition, input light is transmitted to fiber bragg grating 6, optical fiber Bragg from multimode fibre 11
Grating 6 removes a part of covering by hydrofluoric acid solution corrosion, ensures that incident light wave enters the sensing unit of gold-plated film 7, with evanescent wave
Form generate surface plasmon resonance effect, the light of the surface plasmon resonance effect in fibre core and the interface of golden film
Wave exists in the form of plasma wave, and remaining light wave continues to propagate, and reaches the end face of fiber bragg grating 6, through total reflection
After film 10 reflects, surface plasma body resonant vibration occurs for the sensing unit for again passing by gold-plated film 7, makes incident light in sensor internal
Optical path difference doubles, and resonance effect doubles, and improves sensitivity of the SPR resonance wavelengths to extraneous variations in refractive index；Reflected light
It composes the another port Jing Guo 4 two-port of 2X1 couplers and is transmitted to spectrometer 12, received and shown by spectrometer 12, in 7 table of golden film
Silane coupling agent 8 is modified to fix biotin film 9 in face, and biotin film 9 has specific adsorption ability to Streptavidin, works as quilt
When the concentration of survey solution of streptavidin changes, biotin film 9 occurs in various degree with the Streptavidin in solution
Specific binding, causes the refractive index on 7 surface of golden film to change, to which significantly drift occurs for SPR resonance wavelengths, when extraneous temperature
When degree changes, the effective refractive index and screen periods of 6 fibre core of fiber bragg grating can change, so as to cause
Bragg reflection wavelengths shift, and measure the drift value of SPR resonance wavelengths and Bragg reflection wavelengths simultaneously by spectrometer 12,
Realize the high-sensitivity measurement to Streptavidin and temperature biparameter.
The device can realize the key technology that the Streptavidin spr sensor based on fiber bragg grating measures
Have：
1, the SPR sensorgram structure based on fiber bragg grating is to realize the basis of highly sensitive sensing, by multimode fibre,
Fiber bragg grating, total reflection film and be coated with golden film, silane coupling agent, biotin film sensing unit composition.
2, the remaining cladding thickness after fiber bragg grating corrosion.Remaining cladding thickness can influence core mode and be coupled to packet
The energy size of layer and golden film interface, if remaining cladding thickness is too big, core mode is largely leaked out, and influences energy
Transmission；If remaining cladding thickness is too small, surface plasma resonance effect can not be generated.Therefore, fiber bragg grating quilt
Cladding thickness after corrosion should control between 62.5nm-82.5nm.
3, the screen periods of fiber bragg grating.The screen periods of fiber bragg grating influence Bragg reflection wavelengths
Position reduce the wave crest interference between Bragg reflection wavelengths and SPR resonance wavelengths, enhancing measures by changing screen periods
Sensitivity.
4, the thickness and roughness for the golden film that the SPR sensorgram detecting head surface based on fiber bragg grating is coated with.Golden film
Thickness can influence the acuity and extinction ratio of the resonance wavelength resonance peak of SPR, and the degree of roughness on golden film surface can influence surface
The loss of plasma, and then the performance of SPR is influenced, therefore, between the thickness of golden film strictly should control 30nm~50nm, golden film
The root mean square of the roughness on surface should be less than being equal to 3nm.
5, the total reflection film of sensing probe end should control 90% or more the reflectivity of SPR resonance wavelengths, ensure
On golden film surface surface plasma resonance effect occurs for the light energy reflected back again, and resonance effect doubles, and improves and measures spirit
Sensitivity.
In one specific embodiment of the utility model, the stabilization halogen tungsten lamp of Thorlabs, model SLS201 (/M) outputs
Wavelength 300 is between 2600nm；Optoisolator model IO-D-780-VLP, operation wavelength 730-1050nm, isolation exist
Between 34-60dB；Polarization Controller model PLC-900；Multimode fibre cladding diameter is 125 μm；Fiber bragg grating is fine
Core diameter is 62.5 μm, and the remaining cladding thickness after corrosion is 70 μm, centre wavelength 1010nm, axial length 2cm；Golden film
Thickness is 45nm, and surface roughness root mean square is 2.3nm；Total reflection film is 90% to the reflectivity of resonance wavelength.
Claims (1)
1. a kind of Streptavidin spr sensor based on fiber bragg grating, including wideband light source, optoisolator, polarization
Controller, 2X1 couplers, SPR sensorgram probe, spectrometer based on fiber bragg grating, it is characterised in that：Wideband light source
Output end is connected with the input terminal of optoisolator, and the output end of optoisolator is connected with the input terminal of Polarization Controller, 2X1 couplings
One end of device two-port is connected with Polarization Controller, another termination spectrometer, one end of 2X1 coupler Single ports be based on optical fiber
The SPR sensorgram probe of Bragg grating is connected；
It is above-mentioned based on fiber bragg grating SPR sensorgram probe by multimode fibre, fiber bragg grating, total reflection film and
Be coated with golden film, silane coupling agent, biotin film sensing unit composition；125 μm of multimode fibre cladding diameter, fiber bragg grating
62.5 μm of core diameter, the remaining cladding thickness after hydrofluoric acid solution corrodes ensure basic mode coupling between 62.5nm-82.5nm
Close SPR sensorgram area；For the thickness of golden film between 30nm to 50nm, the root mean square of surface roughness is less than or equal to 3nm；Total reflection
Film is to the reflectivity of resonance wavelength 90% or more；Golden film surface modification silane coupling agent fixes biotin film, forms structure
Compact, small sensor.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109596570A (en) * | 2018-10-24 | 2019-04-09 | 昆明理工大学 | A kind of biochemical sensitive system based on Si-based photodetectors |
| CN113049013A (en) * | 2021-03-05 | 2021-06-29 | 桂林电子科技大学 | Novel double-parameter optical fiber biochemical sensor |
-
2018
- 2018-04-02 CN CN201820496307.9U patent/CN207937357U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109596570A (en) * | 2018-10-24 | 2019-04-09 | 昆明理工大学 | A kind of biochemical sensitive system based on Si-based photodetectors |
| CN113049013A (en) * | 2021-03-05 | 2021-06-29 | 桂林电子科技大学 | Novel double-parameter optical fiber biochemical sensor |
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Granted publication date: 20181002 Termination date: 20200402 |