CN207933319U - A kind of protein purification automatic device being suitable for gravity column - Google Patents
A kind of protein purification automatic device being suitable for gravity column Download PDFInfo
- Publication number
- CN207933319U CN207933319U CN201721891580.3U CN201721891580U CN207933319U CN 207933319 U CN207933319 U CN 207933319U CN 201721891580 U CN201721891580 U CN 201721891580U CN 207933319 U CN207933319 U CN 207933319U
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- China
- Prior art keywords
- gravity column
- sample
- component
- fixed plate
- guide rail
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- 230000005484 gravity Effects 0.000 title claims abstract description 106
- 238000001742 protein purification Methods 0.000 title claims abstract description 24
- 238000000746 purification Methods 0.000 claims abstract description 41
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical group C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 6
- 238000010586 diagram Methods 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000011161 development Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Centrifugal Separators (AREA)
Abstract
The utility model discloses a kind of protein purification automatic device being suitable for gravity column, the protein purification automatic device includes holder, it is loaded component, gravity column purification component, receiving unit, second lifting unit and the first lifting unit, the sample-adding component and gravity column purification component are from top to bottom slidably connected on the holder, the component that receives is placed in the gravity purification componentry in lower section, first lifting unit is set between the sample-adding component and the gravity column purification component, for automatically controlling the distance between the sample-adding component and the gravity column purification component；Second lifting unit is set between the guide rail and the gravity column purification component or sample-adding component, for automatically controlling the distance between the sample-adding component and the receiving unit.The utility model provide it is a kind of it is easy to operation, save time, high efficiency, the gravity column purification device that can be automatically controled.
Description
Technical field
The utility model belongs to the purification preparation technology field of biomaterial more particularly to a kind of egg being suitable for gravity column
White matter purifies automatic device.
Background technology
For industrial production, traditional gravity column purification device is simply not proposed to purify a large amount of albumen, AKTA
A variety of chromatographic columns are can be applied not only to protein purifications instruments such as SCG, and elution profile can be observed in real time, at any time adjustment stream
Speed.But this kind of protein purification instrument pipeline is more, longer, prepares longer with time for balancing, if sample size is less, damages
Mistake rate can be higher, and an instrument can only use a kind of purification column, conventional efficient low simultaneously.Traditional gravity column is mainly used in
In terms of scientific research, compared with purifying instrument, have it is easy to operate, save the time, can Simultaneous purification be multiple or multiple proteins
Advantage.
With the development of science and technology, more and more people begin to focus on field of scientific study, all kinds of protein drugs are made with biology
The appearance of product be unable to do without sturdy rigorous development phase, this just needs in technology and equipment aspect to be research staff as far as possible
Facility is provided.At present in terms of albumen gravity column purification there are no the device that can meet easy to operation, high efficiency, automate feature,
The purpose of this utility model be just to provide it is a kind of it is easy to operation, save the time, high efficiency, can be automatically controled gravity column purification dress
It sets.
Invention content
The purpose of this utility model be in view of the problems of the existing technology, provide it is a kind of it is easy to operation, save time, height
Efficiency, the gravity column purification device that can be automatically controled.
To achieve the above object, the technical solution adopted in the utility model is：
A kind of protein purification automatic device being suitable for gravity column, the protein purification automatic device include holder,
Be loaded component, gravity column purification component, receiving unit, the second lifting unit and the first lifting unit, the holder include pedestal and
It is fixedly installed in the guide rail at the pedestal both ends vertically；The sample-adding component includes sample-adding fixed plate and is loaded admittedly mounted on described
Sample injector on fixed board, the sample-adding fixed plate are led by being located in the sample-adding fixed plate compatible with the guide rail first
It is slidably connected to the guide rail to structure, is placed in the guide upper；The gravity column purification component includes gravity column fixed plate
And the gravity column with protein purification function in the gravity column fixed plate, the gravity column fixed plate is by being located at
The second guide frame compatible with the guide rail is slidably connected to the guide rail in the gravity column fixed plate, is placed in described lead
In the middle part of rail；The receiving unit is placed in the underface of the gravity column purification component, including centrifuges tube fixing device and be mounted on institute
State the centrifuge tube on centrifugation tube fixing device；First lifting unit is set to the sample-adding component and the gravity column purification component
Between, for automatically controlling the distance between the sample-adding component and the gravity column purification component；Second lifting unit is set
It is placed between the guide rail and the gravity column purification component or is placed between the guide rail and sample-adding component, for automatically controlling
The distance between the sample-adding component and the receiving unit.
Preferably, rod structure, first guide frame are axle sleeve one to the guide rail in a ring, and described second is oriented to knot
Structure is axle sleeve two；It is intended that it is described annular rod structure and with good guidance quality again can alleviator weight, save
The dosage of material；Guide frame compatible with the guide rail is also convenient for making.
Preferably, the sample-adding fixed plate is equipped with several sample injector location holes for being adapted to the sample injector, described to add
Sample device is placed in the sample injector location hole；It is intended that can purification process be carried out to multiple or multiple proteins simultaneously, carry
High efficiency, and the sample injector can facilitate taking-up, it is easy to operate.
Preferably, the gravity column fixed plate is equipped with the gravity column open slot that several are adapted to the gravity column, described
Gravity column is placed within the gravity column open slot；It is intended that the gravity column in centre position is arranged, space is pipetted
Narrow, pickup is inconvenient, and the design of the open slot is more convenient the gravity column and is taken out from side.
Preferably, the gravity column open slot is equipped with elastic card, for fixing the gravity column.
Preferably, second lifting unit includes first motor, the first screw rod and is arranged in the sample-adding fixed plate
The first threaded hole compatible with first screw rod；The first motor is fixedly installed in the guide upper, passes through control
First screw rod rotates to adjust the sample-adding component to the distance of the receiving unit, it is intended that simple in structure, making
It is easy for installation, it can be achieved that automation control, reduces labor intensity.
Preferably, first lifting unit includes the second motor, the second screw rod and is arranged in the gravity column fixed plate
The second threaded hole compatible with second screw rod；Second motor is fixedly installed in the middle part of the sample-adding fixed plate, is led to
The rotation of the second screw rod of control is crossed to adjust the gravity column purification component to the distance of the sample-adding component, it is intended that
It is simple in structure, it is easy to manufacture and install, it can be achieved that automation control, reduces labor intensity；Motor is placed in middle part, makes the gravity column
Component uniform force, slipping smoothness.
Preferably, the protein purification automatic device is equipped with control unit, and the sample-adding fixed plate lower surface is equipped with the
One distance-sensor, for incuding the distance between the sample-adding fixed plate and the gravity column fixed plate；The gravity column
31 lower surface of fixed plate is equipped with second distance inductor, for incuding the gravity column fixed plate 31 to centrifugation tube fixing device 41
The distance between surface；First motor, the second motor, the first distance-sensor and the second distance inductor and the control
Unit electrical connection processed.
Compared with prior art, the utility model has the beneficial effects that：1, it can be achieved simultaneously to multiple or multiple proteins
Purification work, improve labor efficiency；2, automation control can be achieved, reduce labor intensity.3, apparatus structure is simple, facilitates system
It installs, is at low cost.
Description of the drawings
Fig. 1 is a kind of protein purification automatic device structural schematic diagram being suitable for gravity column of the utility model；
Fig. 2 is the utility model supporting structure schematic diagram；
Fig. 3 is that the utility model is loaded fixed plate structure schematic diagram；
Fig. 4 is the utility model gravity column fixed plate structure schematic diagram；
Fig. 5 is the utility model centrifuge tube fixed rack structure schematic diagram；
Fig. 6 is the protein purification automatic device structural schematic diagram that the utility model another kind is suitable for gravity column；
In figure：1, holder；11, pedestal；12, guide rail；2, it is loaded component；21, it is loaded fixed plate；22, the first guide frame；
23, the first threaded hole；24, sample injector location hole；25, sample injector；3, gravity column purification component；31, gravity column fixed plate；32、
Second guide frame；33, gravity column open slot；34, elastic card；35, the second threaded hole；36, gravity column；4, receiving unit；
41, tube fixing device is centrifuged；42, centrifuge tube location hole；43, centrifuge tube；5, the second lifting unit；51, first motor；52, the first spiral shell
Bar；6, the first lifting unit；61, the second motor；62, the second screw rod；7, the first distance-sensor；8, second distance inductor.
Specific implementation mode
Below in conjunction with the attached drawing in the utility model, the technical solution of the utility model is clearly and completely retouched
It states, it is clear that the described embodiments are only a part of the embodiments of the utility model, instead of all the embodiments.Based on this
Embodiment in utility model, those of ordinary skill in the art obtained under the conditions of not making creative work it is all its
Its embodiment, shall fall within the protection scope of the present invention.
Embodiment one
As shown in Figs. 1-5, a kind of protein purification automatic device being suitable for gravity column, the protein purification fill automatically
It sets including holder 1, sample-adding component 2, gravity column purification component 3, receiving unit 4, the second lifting unit 5 and the first lifting unit 6, it is described
Holder 1 includes pedestal 11 and is fixedly installed in the guide rail 12 at the pedestal both ends vertically；The sample-adding component 2 includes that sample-adding is solid
Fixed board 21 and the sample injector 25 in the sample-adding fixed plate, the sample-adding fixed plate 21 are fixed by being located at the sample-adding
First guide frame 22 compatible with the guide rail 12 is slidably connected and the guide rail 12 on plate 21, is placed on the guide rail 12
Portion；The gravity column purification component 3 includes gravity column fixed plate 31 and has protein in the gravity column fixed plate
The gravity column 36 of function is purified, the gravity column fixed plate 31 is by being located in the gravity column fixed plate 31 and the guide rail 12
Compatible second guide frame 32 is slidably connected and the guide rail 12, is placed in 12 middle part of the guide rail；The receiving unit 4 is set
In the underface of the gravity column purification component 3, including centrifugation tube fixing device 41 and on the centrifugation tube fixing device
Centrifuge tube 43；First lifting unit 6 is set between the sample-adding component 2 and the gravity column purification component 3, for automatic
Control the distance between the sample-adding component 2 and the gravity column purification component 3；Second lifting unit 5 is set to described lead
Between rail 12 and the gravity column purification component 3 or sample-adding component 2, for automatically controlling the sample-adding component 2 and the reception
The distance between component 4.
Preferably, the rod structure in a ring of the guide rail 12 is a stainless pipe；The sample injector is in gravity cylindricality,
Lower end is equipped with rubber hose and control valve；First guide frame 22 is axle sleeve one, and second guide frame 32 is
Axle sleeve two；The sample injector location hole 24 of the sample injector, the sample injector 25 are adapted to there are four being set in the sample-adding fixed plate 21
It is placed in the sample injector location hole 24；The gravity column of the gravity column 36 is adapted to there are four being set in the gravity column fixed plate 31
Open slot 33, the gravity column 36 are placed within the gravity column open slot 33, and the gravity column open slot 33 is equipped with elastic card
Piece 34, for fixing the gravity column 36；Second lifting unit 5 includes that first motor 51, the first screw rod 52 and setting exist
First threaded hole compatible with first screw rod 52 23 in the sample-adding fixed plate 21；The first motor 51 is fixedly mounted
In 12 top of the guide rail, the sample-adding component 2 is adjusted to the receiving unit 4 by controlling the rotation of the first screw rod 52
Distance；First lifting unit 6 include the second motor 61, the second screw rod 62 and be arranged in the gravity column fixed plate with it is described
Second screw rod, 62 compatible second threaded hole 35；Second motor 61 is fixedly installed in 21 lower surface of sample-adding fixed plate
Middle part adjusts the gravity column purification component 3 to the distance of the sample-adding component 2 by controlling the rotation of the second screw rod 62.
Preferably, the protein purification automatic device is equipped with control unit, and 21 lower surface of sample-adding fixed plate is equipped with
First distance-sensor 7, for incuding the distance between the sample-adding fixed plate 21 and the gravity column fixed plate 31；Described
31 lower surface of gravity column fixed plate is equipped with second distance inductor 8, solid to centrifuge tube for incuding the gravity column fixed plate 31
Determine the distance between 41 upper surface of frame；First motor 51, the second motor 61, the first distance-sensor 7 and the second distance
Inductor 8 is electrically connected with described control unit.
When work.According to the difference of experiment, the control unit can pass through the first distance-sensor 7 and second distance
The sensed data of inductor 8 automatically controls the first motor 51 and the second motor 61, the sample-adding component described in adjusting control
2, distance between gravity column purification component 3 and receiving unit 4.
Embodiment two
As shown in fig. 6, the present embodiment place unlike the embodiments above is：The first motor 51 is fixedly installed in institute
12 middle part of guide rail is stated, the gravity column purification component 3 is adjusted to the receiving unit 4 by controlling the rotation of the first screw rod 52
Distance；Second motor 61 is fixedly installed in 31 upper surface middle part of gravity column fixed plate, by controlling the second screw rod
62 rotation come adjust the gravity column purification component 3 to it is described sample-adding component 2 distance.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is appreciated that can these embodiments be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (7)
1. a kind of protein purification automatic device being suitable for gravity column, the protein purification automatic device includes holder, adds
Sample component, gravity column purification component, receiving unit, the second lifting unit and the first lifting unit, it is characterised in that：The holder includes
There is pedestal and is fixedly installed in the guide rail at the pedestal both ends vertically；The sample-adding component includes being loaded fixed plate and mounted on institute
The sample injector in sample-adding fixed plate is stated, the sample-adding fixed plate is adapted by being located in the sample-adding fixed plate with the guide rail
The first guide frame be slidably connected to the guide rail, be placed in the guide upper；The gravity column purification component includes gravity
Column fixed plate and the gravity column with protein purification function in the gravity column fixed plate, the gravity column fixed plate
It is slidably connected to the guide rail by being located at the second guide frame compatible with the guide rail in the gravity column fixed plate, is set
In the middle part of the guide rail；The receiving unit is placed in the underface of the gravity column purification component, including centrifugation tube fixing device and
Centrifuge tube on the centrifugation tube fixing device；First lifting unit is set to the sample-adding component and the gravity column
Between purification componentry, for automatically controlling the distance between the sample-adding component and the gravity column purification component；Described second
Lifting unit is set between the guide rail and the gravity column purification component or is placed between the guide rail and sample-adding component, is used for
Automatically control the distance between the sample-adding component and the receiving unit.
2. a kind of protein purification automatic device being suitable for gravity column according to claim 1, which is characterized in that described
Rod structure, first guide frame are axle sleeve one to guide rail in a ring, and second guide frame is axle sleeve two.
3. a kind of protein purification automatic device being suitable for gravity column according to claim 1, which is characterized in that described
Sample-adding fixed plate is equipped with several sample injector location holes for being adapted to the sample injector, and it is fixed that the sample injector is placed in the sample injector
In the hole of position.
4. a kind of protein purification automatic device being suitable for gravity column according to claim 1, which is characterized in that described
Gravity column fixed plate is equipped with several gravity column open slots for being adapted to the gravity column, and the gravity column is placed in the gravity column
Within open slot.
5. a kind of protein purification automatic device being suitable for gravity column according to claim 4, which is characterized in that described
Gravity column open slot is equipped with elastic card, for fixing the gravity column.
6. a kind of protein purification automatic device being suitable for gravity column according to claim 1, which is characterized in that described
Second lifting unit includes that first motor, the first screw rod and setting are adapted in the sample-adding fixed plate with first screw rod
The first threaded hole；The first motor is fixedly installed in the guide upper, is adjusted by controlling the rotation of the first screw rod
The component that is loaded is to the distance of the receiving unit.
7. a kind of protein purification automatic device being suitable for gravity column according to claim 1, which is characterized in that described
First lifting unit includes that the second motor, the second screw rod and setting are adapted in the gravity column fixed plate with second screw rod
The second threaded hole；Second motor is fixedly installed in the middle part of the sample-adding fixed plate, by the rotation for controlling the second screw rod
To adjust the gravity column purification component to the distance of the sample-adding component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721891580.3U CN207933319U (en) | 2017-12-27 | 2017-12-27 | A kind of protein purification automatic device being suitable for gravity column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721891580.3U CN207933319U (en) | 2017-12-27 | 2017-12-27 | A kind of protein purification automatic device being suitable for gravity column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207933319U true CN207933319U (en) | 2018-10-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721891580.3U Expired - Fee Related CN207933319U (en) | 2017-12-27 | 2017-12-27 | A kind of protein purification automatic device being suitable for gravity column |
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| Country | Link |
|---|---|
| CN (1) | CN207933319U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107987120A (en) * | 2017-12-27 | 2018-05-04 | 湖北普罗金科技有限公司 | A kind of protein purification automatics for being suitable for gravity column |
| CN110760443A (en) * | 2019-11-14 | 2020-02-07 | 嘉兴领宇科技有限公司 | Gravity induction type auxiliary sample adding device and method |
-
2017
- 2017-12-27 CN CN201721891580.3U patent/CN207933319U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107987120A (en) * | 2017-12-27 | 2018-05-04 | 湖北普罗金科技有限公司 | A kind of protein purification automatics for being suitable for gravity column |
| CN110760443A (en) * | 2019-11-14 | 2020-02-07 | 嘉兴领宇科技有限公司 | Gravity induction type auxiliary sample adding device and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190711 Address after: 518000 Xufei Garden Building B 2428, 25 Bagua Second Road, Futian District, Shenzhen City, Guangdong Province Patentee after: Hong Bin Address before: 330098 No. 115 Ziyang Avenue (Administrative Building) 411-28, Nanchang High-tech Industrial Development Zone, Nanchang City, Jiangxi Province Patentee before: NANCHANG PUWEI BIOTECHNOLOGY Co.,Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181002 |