CN107987120A - A kind of protein purification automatics for being suitable for gravity column - Google Patents
A kind of protein purification automatics for being suitable for gravity column Download PDFInfo
- Publication number
- CN107987120A CN107987120A CN201711448428.2A CN201711448428A CN107987120A CN 107987120 A CN107987120 A CN 107987120A CN 201711448428 A CN201711448428 A CN 201711448428A CN 107987120 A CN107987120 A CN 107987120A
- Authority
- CN
- China
- Prior art keywords
- gravity column
- sample
- component
- fixed plate
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
Abstract
The present invention discloses a kind of protein purification automatics for being suitable for gravity column, the protein purification automatics includes stent, it is loaded component, gravity column purification component, receiving unit, second lifting unit and the first lifting unit, the sample-adding component and gravity column purification component are from top to bottom slidably connected on the stent, the component that receives is placed in the gravity purification componentry in lower section, first lifting unit is arranged between the sample-adding component and the gravity column purification component, for automatically controlling sample-adding the distance between the component and the gravity column purification component；Second lifting unit is arranged between the guide rail and the gravity column purification component or sample-adding component, for automatically controlling sample-adding the distance between the component and the receiving unit.The present invention provide it is a kind of it is easy to operation, save time, high efficiency, the gravity column purification device that can be automatically controlled.
Description
Technical field
The invention belongs to the purification preparation technology field of biomaterial, more particularly to a kind of protein for being suitable for gravity column
Purify automatics.
Background technology
For industrial production, traditional gravity column purification device is simply not proposed to purify substantial amounts of albumen, AKTA
Can be applied not only to a variety of chromatographic columns with protein purification instruments such as SCG, and can Real Time Observation elution profile, adjustment stream at any time
Speed.But this kind of protein purification instrument pipeline is more, longer, prepare longer with the time of balance, if sample size is less, damage
Mistake rate can be higher, and an instrument can only use a kind of purification column at the same time, and conventional efficient is low.Traditional gravity column is mainly used in
In terms of scientific research, compared with purifying instrument, have it is easy to operate, save the time, can Simultaneous purification be multiple or multiple proteins
Advantage.
With the development of science and technology, more and more people begin to focus on field of scientific study, all kinds of protein drugs are made with biology
The appearance of product be unable to do without sturdy rigorous development phase, and it is research staff as far as possible that this, which is just needed in technology and equipment aspect,
Facility is provided.It can not meet easy to operation, high efficiency in terms of albumen gravity column purification also at present, automate the device of feature,
It is an object of the invention to provide it is a kind of it is easy to operation, save time, high efficiency, the gravity column purification device that can be automatically controlled.
The content of the invention
In order to solve the problems in the prior art, the purpose of the present invention is to, there is provided one kind is easy to operation, saves the time, efficiently
Rate, the gravity column purification device that can be automatically controlled.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of protein purification automatics for being suitable for gravity column, the protein purification automatics include stent,
Be loaded component, gravity column purification component, receiving unit, the second lifting unit and the first lifting unit, the stent include base and
The guide rail at the base both ends is fixedly installed in vertically；The sample-adding component includes sample-adding fixed plate and is loaded admittedly installed in described
Sample injector on fixed board, the sample-adding fixed plate are led by being located at be adapted in the sample-adding fixed plate with the guide rail first
The guide rail is slidably connected to structure, is placed in the guide upper；The gravity column purification component includes gravity column fixed plate
And there is the gravity column of protein purification in the gravity column fixed plate, the gravity column fixed plate is by being located at
The second guide frame being adapted in the gravity column fixed plate with the guide rail is slidably connected to the guide rail, is placed in described lead
In the middle part of rail；The receiving unit is placed in the underface of the gravity column purification component, including centrifugation tube fixing device and installed in institute
State the centrifuge tube on centrifugation tube fixing device；First lifting unit is arranged at the sample-adding component and the gravity column purification component
Between, for automatically controlling sample-adding the distance between the component and the gravity column purification component；Second lifting unit is set
It is placed between the guide rail and the gravity column purification component or is placed between the guide rail and sample-adding component, for automatically controls
Sample-adding the distance between the component and the receiving unit.
Preferably, rod structure, first guide frame are axle sleeve one to the guide rail in a ring, and described second is oriented to knot
Structure is axle sleeve two；It is intended that it is described annular rod structure and with good guidance quality again can alleviator weight, save
The dosage of material；The guide frame being adapted with the guide rail is also convenient for making.
Preferably, the sample-adding fixed plate is equipped with the sample injector location hole of several adaptation sample injectors, described to add
Sample device is placed in the sample injector location hole；It is intended that purification process can be carried out to multiple or multiple proteins at the same time, carry
High efficiency, and the sample injector can conveniently take out, it is easy to operate.
Preferably, the gravity column fixed plate is equipped with the gravity column open slot of several adaptation gravity columns, described
Gravity column is placed within the gravity column open slot；It is intended that being arranged on the gravity column in centre position, space is pipetted
Narrow and small, pickup is inconvenient, and the design of the open slot is more convenient the gravity column and is taken out from side.
Preferably, the gravity column open slot is equipped with elastic card, for fixing the gravity column.
Preferably, second lifting unit includes the first motor, the first screw rod and is arranged in the sample-adding fixed plate
The first threaded hole being adapted with first screw rod；First motor is fixedly installed in the guide upper, passes through control
First screw rod is rotated to adjust the sample-adding component to the distance of the receiving unit, it is intended that simple in structure, making
It is easy for installation, it can be achieved that automated control, reduces labor intensity.
Preferably, first lifting unit includes the second motor, the second screw rod and is arranged in the gravity column fixed plate
The second threaded hole being adapted with second screw rod；Second motor is fixedly installed in the middle part of the sample-adding fixed plate, is led to
Cross control the second screw rod rotation come adjust the gravity column purification component to it is described sample-adding component distance, it is intended that
It is simple in structure, it is easy to manufacture and install, it can be achieved that automated control, reduces labor intensity；Motor is placed in middle part, makes the gravity column
Component uniform force, slipping smoothness.
Preferably, the protein purification automatics is equipped with control unit, and the sample-adding fixed plate lower surface is equipped with the
One distance-sensor, for sensing sample-adding the distance between the fixed plate and the gravity column fixed plate；The gravity column
Fixed plate lower surface is equipped with second distance inductor, for sense the gravity column fixed plate to centrifuge tube fixing device upper surface it
Between distance；First motor, the second motor, the first distance-sensor and second distance inductor and described control unit
It is electrically connected.
Compared with prior art, the beneficial effects of the invention are as follows：1st, can be achieved at the same time to multiple or multiple proteins pure
Chemical industry is made, and improves labor efficiency；2nd, automated control can be achieved, reduce labor intensity.3rd, apparatus structure is simple, conveniently makes peace
Dress, cost are low.
Brief description of the drawings
Fig. 1 is a kind of protein purification automatics structure diagram for being suitable for gravity column of the present invention；
Fig. 2 is supporting structure schematic diagram of the present invention；
Fig. 3 is present invention sample-adding fixed plate structure schematic diagram；
Fig. 4 is gravity column fixed plate structure schematic diagram of the present invention；
Fig. 5 is centrifuge tube fixed rack structure schematic diagram of the present invention；
Fig. 6 is the protein purification automatics structure diagram that another kind of the present invention is suitable for gravity column；
In figure：1st, stent；11st, base；12nd, guide rail；2nd, it is loaded component；21st, it is loaded fixed plate；22nd, the first guide frame；
23rd, the first threaded hole；24th, sample injector location hole；25th, sample injector；3rd, gravity column purification component；31st, gravity column fixed plate；32、
Second guide frame；33rd, gravity column open slot；34th, elastic card；35th, the second threaded hole；36th, gravity column；4th, receiving unit；
41st, tube fixing device is centrifuged；42nd, centrifuge tube location hole；43rd, centrifuge tube；5th, the second lifting unit；51st, the first motor；52nd, the first spiral shell
Bar；6th, the first lifting unit；61st, the second motor；62nd, the second screw rod；7th, the first distance-sensor；8th, second distance inductor.
Embodiment
Below in conjunction with the attached drawing in the present invention, technical scheme is clearly and completely described, it is clear that
Described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on the implementation in the present invention
Example, all other embodiment that those of ordinary skill in the art are obtained under the conditions of creative work is not made, belongs to
The scope of protection of the invention.
Embodiment one
As shown in Figs. 1-5, a kind of protein purification automatics for being suitable for gravity column, the protein purification fill automatically
Put including stent 1, sample-adding component 2, gravity column purification component 3, receiving unit 4, the second lifting unit 5 and the first lifting unit 6, it is described
Stent 1 includes base 11 and is fixedly installed in the guide rail 12 at the base both ends vertically；It is solid that the sample-adding component 2 includes sample-adding
Fixed board 21 and the sample injector 25 in the sample-adding fixed plate, the sample-adding fixed plate 21 are fixed by being located at the sample-adding
The first guide frame 22 being adapted on plate 21 with the guide rail 12 is slidably connected and the guide rail 12, is placed on the guide rail 12
Portion；The gravity column purification component 3 includes gravity column fixed plate 31 and has protein in the gravity column fixed plate
The gravity column 36 of function is purified, the gravity column fixed plate 31 is by being located in the gravity column fixed plate 31 and the guide rail 12
The second guide frame 32 being adapted is slidably connected and the guide rail 12, is placed in the middle part of guide rail 12；The receiving unit 4 is put
In the underface of the gravity column purification component 3, including centrifuge tube fixing device 41 and centrifuged installed in described on tube fixing device
Centrifuge tube 43；First lifting unit 6 is arranged between the sample-adding component 2 and the gravity column purification component 3, for automatic
Control sample-adding the distance between the component 2 and the gravity column purification component 3；Second lifting unit 5 is arranged at described lead
Between rail 12 and the gravity column purification component 3 or sample-adding component 2, for automatically controlling the sample-adding component 2 and the reception
The distance between component 4.
Preferably, the guide rail 12 rod structure in a ring, is a stainless pipe；The sample injector is in gravity cylindricality,
Lower end is equipped with rubber hose and control valve；First guide frame 22 is axle sleeve one, and second guide frame 32 is
Axle sleeve two；The sample-adding fixed plate 21 is equipped with the sample injector location hole 24 of four adaptation sample injectors, the sample injector 25
It is placed in the sample injector location hole 24；The gravity column fixed plate 31 is equipped with the gravity column of four adaptation gravity columns 36
Open slot 33, the gravity column 36 are placed within the gravity column open slot 33, and the gravity column open slot 33 is equipped with elastic card
Piece 34, for fixing the gravity column 36；Second lifting unit 5 includes the first motor 51, the first screw rod 52 and is arranged on
The first threaded hole 23 being adapted in the sample-adding fixed plate 21 with first screw rod 52；First motor 51 is fixedly mounted
In 12 top of guide rail, the sample-adding component 2 is adjusted to the receiving unit 4 by controlling the rotation of the first screw rod 52
Distance；First lifting unit 6 include the second motor 61, the second screw rod 62 and be arranged in the gravity column fixed plate with it is described
The second threaded hole 35 that second screw rod 62 is adapted；Second motor 61 is fixedly installed in 21 lower surface of the sample-adding fixed plate
Middle part, the gravity column purification component 3 is adjusted to the distance of the sample-adding component 2 by controlling the rotation of the second screw rod 62.
Preferably, the protein purification automatics is equipped with control unit, and 21 lower surface of the sample-adding fixed plate is equipped with
First distance-sensor 7, for sensing sample-adding the distance between the fixed plate 21 and the gravity column fixed plate 31；Described
31 lower surface of gravity column fixed plate is equipped with second distance inductor 8, consolidates for sensing the gravity column fixed plate 31 to centrifuge tube
Determine the distance between 41 upper surface of frame；First motor 51, the second motor 61, the first distance-sensor 7 and the second distance
Inductor 8 is electrically connected with described control unit.
During work.According to the difference of experiment, the control unit can pass through the first distance-sensor 7 and second distance
The sensed data of inductor 8 automatically controls first motor 51 and the second motor 61, the sample-adding component described in adjustment control
2nd, distance between gravity column purification component 3 and receiving unit 4.
Embodiment two
As shown in fig. 6, the present embodiment part unlike the embodiments above is：First motor 51 is fixedly installed in institute
The middle part of guide rail 12 is stated, the gravity column purification component 3 is adjusted to the receiving unit 4 by controlling the rotation of the first screw rod 52
Distance；Second motor 61 is fixedly installed in 31 upper surface middle part of gravity column fixed plate, by controlling the second screw rod
62 rotation come adjust the gravity column purification component 3 to it is described sample-adding component 2 distance.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of changes, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of protein purification automatics for being suitable for gravity column, the protein purification automatics includes stent, adds
Sample component, gravity column purification component, receiving unit, the second lifting unit and the first lifting unit, it is characterised in that：The stent includes
There is base and be fixedly installed in the guide rail at the base both ends vertically；The sample-adding component includes sample-adding fixed plate and installed in institute
The sample injector in sample-adding fixed plate is stated, the sample-adding fixed plate is adapted by being located in the sample-adding fixed plate with the guide rail
The first guide frame be slidably connected to the guide rail, be placed in the guide upper；The gravity column purification component includes gravity
Column fixed plate and there is the gravity column of protein purification, the gravity column fixed plate in the gravity column fixed plate
The guide rail is slidably connected to by being located at the second guide frame being adapted in the gravity column fixed plate with the guide rail, is put
In the middle part of the guide rail；The receiving unit is placed in the underface of the gravity column purification component, including centrifugation tube fixing device and
Centrifuge tube on the centrifugation tube fixing device；First lifting unit is arranged at the sample-adding component and the gravity column
Between purification componentry, for automatically controlling sample-adding the distance between the component and the gravity column purification component；Described second
Lifting unit is arranged between the guide rail and the gravity column purification component or is placed between the guide rail and sample-adding component, is used for
Automatically control sample-adding the distance between the component and the receiving unit.
2. a kind of protein purification automatics for being suitable for gravity column according to claim 1, it is characterised in that described
Rod structure, first guide frame are axle sleeve one to guide rail in a ring, and second guide frame is axle sleeve two.
3. a kind of protein purification automatics for being suitable for gravity column according to claim 1, it is characterised in that described
The sample injector location hole that fixed plate is equipped with several adaptation sample injectors is loaded, the sample injector, which is placed in the sample injector, to be determined
In the hole of position.
4. a kind of protein purification automatics for being suitable for gravity column according to claim 1, it is characterised in that described
Gravity column fixed plate is equipped with the gravity column open slot of several adaptation gravity columns, and the gravity column is placed in the gravity column
Within open slot.
5. a kind of protein purification automatics for being suitable for gravity column according to claim 4, it is characterised in that described
Gravity column open slot is equipped with elastic card, for fixing the gravity column.
6. a kind of protein purification automatics for being suitable for gravity column according to claim 1, it is characterised in that described
Second lifting unit includes the first motor, the first screw rod and is arranged in the sample-adding fixed plate to be adapted with first screw rod
The first threaded hole；First motor is fixedly installed in the guide upper, is adjusted by controlling the rotation of the first screw rod
The component that is loaded is to the distance of the receiving unit.
7. a kind of protein purification automatics for being suitable for gravity column according to claim 1, it is characterised in that described
First lifting unit includes the second motor, the second screw rod and is arranged in the gravity column fixed plate to be adapted with second screw rod
The second threaded hole；Second motor is fixedly installed in the middle part of the sample-adding fixed plate, by the rotation for controlling the second screw rod
To adjust the gravity column purification component to the distance of the sample-adding component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711448428.2A CN107987120A (en) | 2017-12-27 | 2017-12-27 | A kind of protein purification automatics for being suitable for gravity column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711448428.2A CN107987120A (en) | 2017-12-27 | 2017-12-27 | A kind of protein purification automatics for being suitable for gravity column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107987120A true CN107987120A (en) | 2018-05-04 |
Family
ID=62043007
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711448428.2A Withdrawn CN107987120A (en) | 2017-12-27 | 2017-12-27 | A kind of protein purification automatics for being suitable for gravity column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107987120A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002053256A1 (en) * | 2001-01-05 | 2002-07-11 | Pro-Chem, Inc. | Devices and methods for purification |
| US20090294385A1 (en) * | 2005-05-17 | 2009-12-03 | Universal Bio Research Co., Ltd | Filtration method, filter-incorporated tip, and filtration device |
| CN203048837U (en) * | 2013-01-22 | 2013-07-10 | 扬州艾迪生物科技有限公司 | Batched protein separation and purification device |
| CN103827137A (en) * | 2011-08-26 | 2014-05-28 | 株式会社百奥尼 | Protein synthesis kit, and method for expressing and extracting proteins using automatic extraction equipment |
| CN103881892A (en) * | 2014-01-06 | 2014-06-25 | 艾康生物技术(杭州)有限公司 | Nucleic acid extractor, and nucleic acid extractor heater and heating method |
| CN205368380U (en) * | 2016-02-02 | 2016-07-06 | 广州康昕瑞基因健康科技有限公司 | Reagent workstation with Z axle anticollision institution |
| CN206666433U (en) * | 2017-04-21 | 2017-11-24 | 深圳市国创纳米抗体技术有限公司 | A kind of protein purification device for being suitable for gravity post |
| CN206692665U (en) * | 2017-03-28 | 2017-12-01 | 东莞市佰韧五金电子科技有限公司 | Nucleic acid purification equipment |
| CN207933319U (en) * | 2017-12-27 | 2018-10-02 | 南昌普威生物科技有限公司 | A kind of protein purification automatic device being suitable for gravity column |
-
2017
- 2017-12-27 CN CN201711448428.2A patent/CN107987120A/en not_active Withdrawn
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002053256A1 (en) * | 2001-01-05 | 2002-07-11 | Pro-Chem, Inc. | Devices and methods for purification |
| US20090294385A1 (en) * | 2005-05-17 | 2009-12-03 | Universal Bio Research Co., Ltd | Filtration method, filter-incorporated tip, and filtration device |
| CN103827137A (en) * | 2011-08-26 | 2014-05-28 | 株式会社百奥尼 | Protein synthesis kit, and method for expressing and extracting proteins using automatic extraction equipment |
| CN203048837U (en) * | 2013-01-22 | 2013-07-10 | 扬州艾迪生物科技有限公司 | Batched protein separation and purification device |
| CN103881892A (en) * | 2014-01-06 | 2014-06-25 | 艾康生物技术(杭州)有限公司 | Nucleic acid extractor, and nucleic acid extractor heater and heating method |
| CN205368380U (en) * | 2016-02-02 | 2016-07-06 | 广州康昕瑞基因健康科技有限公司 | Reagent workstation with Z axle anticollision institution |
| CN206692665U (en) * | 2017-03-28 | 2017-12-01 | 东莞市佰韧五金电子科技有限公司 | Nucleic acid purification equipment |
| CN206666433U (en) * | 2017-04-21 | 2017-11-24 | 深圳市国创纳米抗体技术有限公司 | A kind of protein purification device for being suitable for gravity post |
| CN207933319U (en) * | 2017-12-27 | 2018-10-02 | 南昌普威生物科技有限公司 | A kind of protein purification automatic device being suitable for gravity column |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207933319U (en) | A kind of protein purification automatic device being suitable for gravity column | |
| CN202777924U (en) | Multi-channel rotary column chromatographical purification device | |
| CN107987120A (en) | A kind of protein purification automatics for being suitable for gravity column | |
| CN104646176A (en) | Manual magnetic solid extractor | |
| CN206666433U (en) | A kind of protein purification device for being suitable for gravity post | |
| CN218811761U (en) | Portable detection device for pathogen detection antigen | |
| CN201454152U (en) | Positive-pressure solid-phase extracting device | |
| CN203678012U (en) | Splittable chromatographic column | |
| CN212008059U (en) | Solid phase extraction instrument | |
| CN202886172U (en) | Rapid air-blowing concentration device | |
| CN109481962A (en) | A kind of automatic filling of chromatographic column and elution multifunctional combined apparatus | |
| CN206838132U (en) | A kind of rotary glass chromatogram column fixer | |
| CN207123422U (en) | A kind of support frame of air sampler | |
| CN206818736U (en) | Sampling device is sequenced in a kind of automatic gene | |
| CN106345141B (en) | A kind of chromatographic column frame | |
| CN215985909U (en) | A solid-phase extraction device for food detects | |
| CN208511923U (en) | A kind of Solid Phase Extraction frame | |
| CN2287720Y (en) | Sampling frame for environment microbe | |
| CN208803009U (en) | Protein purification device | |
| CN209076100U (en) | A kind of boundling chromatography machine | |
| CN209680146U (en) | A kind of bracket of chromatographic column | |
| CN217111690U (en) | Novel water quality sulfide acidification blowing instrument | |
| CN208852436U (en) | A kind of spherical concentrator | |
| CN207126546U (en) | A kind of senior chemistry experiment titration outfit | |
| CN208532766U (en) | A kind of bottom support type magnetic force rack device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180504 |
|
| WW01 | Invention patent application withdrawn after publication |