CN207918876U - A kind of cell culture container - Google Patents

Abstract

The utility model discloses a kind of cell culture containers, including bottle, both sides above the bottle are respectively arranged with the first branch pipe and the second branch pipe, it is provided with lid on first branch pipe, it is provided with inflation mechanism on second branch pipe, the bottle is fixedly arranged above fixed block, through hole is set at the center of the fixed block, the fixed block is fixedly arranged above fixed cylinder corresponding with hole site is run through, the bottle is internally provided with agitating shaft, the upper end of the agitating shaft is rotatablely connected through through hole to the internal stretch of fixed cylinder and with the inner top of fixed cylinder.In the utility model, by the way that multiple support plates are arranged, multiple circular holes are set on support plate, starter motor can make multiple support plates rotate, and cell culture liquid energy generates vortex by multiple circular holes, and suspension cell is prevented to be attached on the inner wall of bottle, bottle need not manually be shaken, deep layer is passed through carbon dioxide and air is more convenient, greatly improves working efficiency, and practicability is stronger.

Description

A kind of cell culture container

Technical field

The utility model is related to technical field of cell culture more particularly to a kind of cell culture containers.

Background technology

Suspend culture refer to it is a kind of in the fluid nutrient medium for constantly being stirred or being shaken, culture it is unicellular and it is small carefully The tissue culture system of born of the same parents group, is a kind of training method of non-anchorage-dependent cell.Certain anchorage-dependent cells are by suitable The method culture should can be also used with selection.It is relatively simple to increase suspension culture scale, as long as increasing volume.It is deep Degree needs to stir culture medium, more than 10 centimetres more than 5 millimeters, it is also necessary to which deep layer is passed through carbon dioxide and air, enough to ensure Gas exchanges.

In the prior art, general culture bottle is when suspend culture, be all it is artificial shake culture bottle, it is artificial shake compared with For trouble, and while shaking, into culture bottle plus culture solution is inconvenient, due to being manually to shake, can not deep layer be passed through Carbon dioxide and air.

Utility model content

Purpose of the utility model is to solve in the prior art when suspend culture, it is artificial shake culture bottle compared with For troublesome problem, and a kind of cell culture container proposed.

To achieve the goals above, the utility model uses following technical solution：

A kind of cell culture container, including bottle, the both sides above the bottle are respectively arranged with the first branch pipe and second Branch pipe is provided with lid on first branch pipe, inflation mechanism is provided on second branch pipe, the top of the bottle is fixed There is fixed block, through hole is set at the center of the fixed block, being fixedly arranged above for the fixed block is corresponding with through hole site Fixed cylinder, the bottle is internally provided with agitating shaft, and inside of the through hole to fixed cylinder is run through in the upper end of the agitating shaft Extend and be rotatablely connected with the inner top of fixed cylinder, the lower end of the agitating shaft is provided with rabbling mechanism, is set in the fixed block It is equipped with driving mechanism corresponding with agitating shaft.

Preferably, the inflation mechanism includes rubber stopper, and the rubber stopper is located at the port of the second branch pipe, the bottle Interior setting snorkel, the snorkel are in bending, and the lower end of the snorkel extends to the interior bottom of bottle, the snorkel The other end extend to outside bottle through rubber stopper.

Preferably, the driving mechanism includes first bevel gear, and the first bevel gear is arranged in the upper end of agitating shaft, institute The upper end for stating fixed cylinder inner wall side is provided with trepanning, and the outer wall of the fixed cylinder is fixed with motor corresponding with position of opening, Be interspersed with connecting shaft in the trepanning, one end of the connecting shaft extend to outside fixed cylinder through trepanning and with the driving end of motor Connection, the other end of the connecting shaft extend into fixed cylinder and are fixedly connected with second bevel gear through trepanning, and described second Bevel gear engages connection with first bevel gear.

Preferably, the madial wall of the agitating shaft and through hole is rotatablely connected.

Preferably, the connecting shaft and the madial wall of trepanning are rotatablely connected.

Preferably, the rabbling mechanism includes multiple support plates, and multiple support plates are circumferentially positioned at the lower end of agitating shaft, institute It states and is provided with multiple circular holes on support plate.

In the utility model, by the way that multiple support plates are arranged, multiple circular holes are set on support plate, and starter motor can make multiple Support plate rotates, and cell culture liquid energy generates vortex by multiple circular holes, prevents suspension cell to be attached on the inner wall of bottle, is not required to Bottle is manually shaken, deep layer is passed through carbon dioxide and air is more convenient, greatly improves working efficiency, and practicability is stronger, value It must widely popularize.

Description of the drawings

Fig. 1 be the utility model proposes a kind of cell culture container structural schematic diagram；

Fig. 2 be the utility model proposes a kind of cell culture container in structural schematic diagram at A.

In figure：1 lid, 2 first branch pipes, 3 bottles, 4 support plates, 5 circular holes, 6 through holes, 7 fixed blocks, 8 agitating shafts, 9 second Branch pipe, 10 rubber stoppers, 11 snorkels, 12 first bevel gears, 13 fixed cylinders, 14 second bevel gears, 15 connecting shafts, 16 motors, 17 Trepanning.

Specific implementation mode

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.

Referring to Fig.1-2, the both sides of a kind of cell culture container, including bottle 3,3 top of bottle are respectively arranged with first Pipe 2 and the second branch pipe 9 are provided with lid 1 on first branch pipe 2, and inflation mechanism is provided on the second branch pipe 9, and the top of bottle 3 is solid Surely there is fixed block 7, through hole 6 is set at the center of fixed block 7, being fixedly arranged above for fixed block 7 is corresponding with 6 position of through hole Fixed cylinder 13, bottle 3 are internally provided with agitating shaft 8, and the upper end of agitating shaft 8 is prolonged through through hole 6 to the inside of fixed cylinder 13 Stretch and be rotatablely connected with the inner top of fixed cylinder 13, the lower end of agitating shaft 8 is provided with rabbling mechanism, be provided in fixed block 7 with 8 corresponding driving mechanism of agitating shaft.

In the utility model, inflation mechanism includes rubber stopper 10, and rubber stopper 10 is located at the port of the second branch pipe 9, bottle 3 Interior setting snorkel 11, snorkel 11 are in bending, and the lower end of snorkel 11 extends to the interior bottom of bottle 3, snorkel 11 The other end extends through rubber stopper 10 to outside bottle 3, when cultivating suspension cell, deep layer is facilitated to be passed through carbon dioxide and air, Driving mechanism includes first bevel gear 12, and first bevel gear 12 is arranged in the upper end of agitating shaft 8,13 inner wall side of fixed cylinder it is upper End is provided with trepanning 17, and the outer wall of fixed cylinder 13 is fixed with motor 16 corresponding with 17 position of trepanning, the company of being interspersed in trepanning 17 One end of spindle 15, connecting shaft 15 extends and is connect with the driving end of motor 16, connecting shaft to outside fixed cylinder 13 through trepanning 17 15 other end extends into fixed cylinder 13 through trepanning 17 and is fixedly connected with second bevel gear 14, second bevel gear 14 and The engagement connection of one bevel gear 12, starter motor 16 can make support plate 4 rotate, so as to stir the cell culture fluid in bottle 3, Suspension cell is prevented to be attached on the inner wall of bottle 3, agitating shaft 8 and the madial wall of through hole 6 are rotatablely connected, and prevent agitating shaft 8 It is shaken when rotation, bottle 3 is caused integrally to shake, connecting shaft 15 and the madial wall of trepanning 17 are rotatablely connected, and prevent 15 turns of connecting shaft It is shaken in trepanning 17 when dynamic, rabbling mechanism includes multiple support plates 4, and multiple support plates 4 are circumferentially positioned at the lower end of agitating shaft 8, branch Multiple circular holes 5 are provided on plate 4, while support plate 4 rotates, cell culture liquid energy generates vortex by circular hole 5, prevents from suspending thin Born of the same parents are attached on the inner wall of bottle 3.

In the utility model, when in use, it is first turned on lid 1, cell culture fluid is poured into bottle by the first branch pipe 2 3 inside, due to suspending, culture needs ceaselessly shake culture liquid, and living environment, man-hour manually hand-held bottle 3 are provided for suspension cell It shakes cumbersome, only needs starter motor 16 at this time so that connecting shaft 15 and second bevel gear 14 rotate, due to the second cone tooth Wheel 14 engages connection with first bevel gear 12, and the rotation of second bevel gear 14 drives first bevel gear 12 to rotate so that agitating shaft 8 Rotation, while agitating shaft 8 rotates, support plate 4 and then rotates, and culture solution is ceaselessly stirred, culture solution on support plate 4 by being arranged Circular hole 5 generate vortex so that culture liquid energy adequately stir, avoid suspension cell from being attached on the inner wall of bottle 3, cultivating When suspension cell, deep layer is needed to be passed through carbon dioxide and air, at this point, only need to lead to carbon dioxide and air by snorkel 11 Enter in culture solution, operation is relatively simple, provides working efficiency.

More than, the only preferable specific implementation mode of the utility model, but the scope of protection of the utility model is not limited to In this, any one skilled in the art within the technical scope disclosed by the utility model, according to the utility model Technical solution and its utility model design be subject to equivalent substitution or change, should all cover the scope of protection of the utility model it It is interior.

Claims (6)

1. a kind of cell culture container, including bottle（3）, which is characterized in that the bottle（3）The both sides of top are respectively arranged with First branch pipe（2）With the second branch pipe（9）, first branch pipe（2）On be provided with lid（1）, second branch pipe（9）Upper setting There are inflation mechanism, the bottle（3）Be fixedly arranged above fixed block（7）, the fixed block（7）Center at be arranged through hole （6）, the fixed block（7）Be fixedly arranged above and through hole（6）The corresponding fixed cylinder in position（13）, the bottle（3）It is interior Portion is provided with agitating shaft（8）, the agitating shaft（8）Upper end run through through hole（6）To fixed cylinder（13）Internal stretch and with Fixed cylinder（13）Inner top rotation connection, the agitating shaft（8）Lower end be provided with rabbling mechanism, the fixed block（7）It is interior It is provided with and agitating shaft（8）Corresponding driving mechanism.

2. a kind of cell culture container according to claim 1, which is characterized in that the inflation mechanism includes rubber stopper （10）, the rubber stopper（10）Positioned at the second branch pipe（9）Port at, the bottle（3）Interior setting snorkel（11）, described logical Tracheae（11）In bending, the snorkel（11）Lower end to bottle（3）Interior bottom extend, the snorkel（11）It is another Rubber stopper is run through in one end（10）To bottle（3）Outer extension.

3. a kind of cell culture container according to claim 1, which is characterized in that the driving mechanism includes the first cone tooth Wheel（12）, the first bevel gear（12）It is arranged in agitating shaft（8）Upper end, the fixed cylinder（13）The upper end of inner wall side is set It is equipped with trepanning（17）, the fixed cylinder（13）Outer wall be fixed with and trepanning（17）The corresponding motor in position（16）, the trepanning （17）Inside it is interspersed with connecting shaft（15）, the connecting shaft（15）One end run through trepanning（17）To fixed cylinder（13）It is outer extension and with Motor（16）Driving end connection, the connecting shaft（15）The other end run through trepanning（17）To fixed cylinder（13）Interior extension is simultaneously solid Surely it is connected with second bevel gear（14）, the second bevel gear（14）With first bevel gear（12）Engagement connection.

4. a kind of cell culture container according to claim 1, which is characterized in that the agitating shaft（8）With through hole（6） Madial wall rotation connection.

5. a kind of cell culture container according to claim 3, which is characterized in that the connecting shaft（15）With trepanning（17） Madial wall rotation connection.

6. a kind of cell culture container according to claim 1, which is characterized in that the rabbling mechanism includes multiple support plates （4）, multiple support plates（4）It is circumferentially positioned at agitating shaft（8）Lower end, the support plate（4）On be provided with multiple circular holes（5）.