CN207911464U - A kind of rice takes root ability identification apparatus - Google Patents

Abstract

The utility model discloses a kind of rice to take root ability identification apparatus, includes taking root device and equipped with the culture apparatus of nutriment equipped with solid state rheology matrix；And it takes root device and is placed in culture apparatus；Wherein, it is uniformly with holes and in substantially symmetrical about its central axis to take root device, axial plane is divided into symmetrically and the equal two parts of surface area in arbitrary：Area and unobstructed zone are hindered, the inner surface in area and/or outer surface is hindered to be evenly distributed with barrier layer；The center of solid state rheology matrix is equipped with index aperture, and rice seedling plantation is at index aperture；There are several ratios of taking root of barrier layer side and without hindrance layer side to be used as the index that this plant of plant takes root ability on same strain rice by calculating, solve in the prior art rice take root ability identification it is cumbersome, it is less reproducible, the problems such as can not implementing on a large scale, realizes that extensive, rapidly and accurately progress rice takes root ability identification.

Description

Rice rooting capacity identification device

Technical Field

The utility model belongs to the technical field of the crops are planted, concretely relates to root ability appraisal device is pricked to rice effectively improves the efficiency and the accuracy of root ability appraisal are pricked to rice.

Background

At present, the identification method of the root-pricking capacity of rice mainly comprises two methods: the heavy pressing method and the wax layer method. The recompression method, originally invented by Materechaeta in 1991, is shown in FIG. 1, and its principle is to form a layer of compact sand column by the compression of a weight to simulate the plough bottom layer, but the main disadvantages of the method are: 1) the pressure of the sand columns is consistent from top to bottom, which is far from the actual soil condition; 2) the installation of the device is difficult, and the pressure between different blocks in the same sand column and the pressure between different sand columns are difficult to keep consistent; 3) the viability of the seed itself can have a significant impact on the results. The wax layer method, which is invented by Yu et al in 1995 as a method for identifying the rooting ability by simulating the plough bottom layer with the wax layer, is shown in figure 2, and is improved compared with the repressing method, Ray et al in 1996 and Zheng et al in 2000 have also conducted some researches to locate some quantitative trait gene sites for controlling the rooting ability, but there are few related reports after 2005, and the reports are less frequent in China. The main possibility is that the method has more defects, such as: 1) the method calculates the ratio of the number of roots penetrating through the wax layer to the total number of roots, so that the length difference of different roots of the same individual and the total number difference of different individuals have great influence on the result; 2) the sealing between the wax layer and the tank body is difficult, the blocking of the connecting area is weakest, and because the tank body limits the lateral growth of roots, a plurality of roots can penetrate out of the connecting position between the tank body and the wax layer, thereby influencing the accuracy of the result; 3) the installation of the screening device is not easy, and the sand is easy to collapse, thereby influencing the stability of the wax layer.

SUMMERY OF THE UTILITY MODEL

In order to overcome the above defects of the prior art, the present invention provides a rice rooting capacity identification device.

The inventor develops a rice rooting capacity identification device on the basis of absorbing previous experience, and by calculating the root rooting ratio value of one side with a barrier layer and one side without the barrier layer on the same rice as an index of the root rooting capacity of the plant, the problems that the rice rooting capacity identification operation is complicated, the repeatability is poor, large-scale implementation cannot be realized and the like in the prior art are solved, and the rice rooting capacity identification is realized rapidly and accurately in a large scale.

On the other hand, the traditional method needs a special device and has complicated working procedures; the utility model discloses what compare is that the root of same trunk rice both sides only needs the root device of pricking of even foraminiferous and center pin symmetry. Because one side of the root pricking device without the barrier layer is provided with the normal holes, nutrient substances can be fully communicated with the environment through moisture and nutrition, the growth state of the plants is not different from that of the plants in the ordinary field or nutrient solution cultivation, and compared with the prior art, the root pricking device tends to be consistent and stable in both growth environment and physiological process, and the result is closer to the real natural state.

The above object of the utility model is realized through following technical scheme:

a rice rooting ability evaluating device comprising: a root pricking device filled with a solid culture medium and a culture device filled with nutrient substances; the root pricking device is arranged in the culture device; wherein,

the root pricking device is even with holes and is symmetrical about a central axis, and is divided into two parts with symmetrical and equal surface areas along any central axial plane: the barrier comprises a barrier area and an unobstructed area, wherein barrier layers are uniformly distributed on the inner surface and/or the outer surface of the barrier area; the central position filled with the solid culture medium is provided with a mark hole;

and rice seedlings are planted at the mark holes, and the planting depth falls into the position of 1-3 cm in the solid culture medium.

Further, the cross-section of the rooting device comprises a circle, an ellipse or a square.

Furthermore, the thickness of the blocking layer of the root pricking device is 2-5 mm.

Further, the barrier layer of the rooting device is a wax layer and comprises a mixture of vaseline and paraffin, and the vaseline accounts for 20% -80% of the total mass of the mixture of vaseline and paraffin. It should be noted that, in the technical scheme of the utility model, the root-tying capabilities of different rice varieties have great differences, for example, the rice variety with extremely strong root-tying capability is selected as the root-tying device with lower vaseline concentration ratio; the root-tying device with a higher vaseline concentration ratio is selected for rice varieties with extremely weak root-tying capability, the melting temperature is changed according to the vaseline ratio, and the higher the vaseline ratio is, the lower the temperature is. In addition, since the hardness of the barrier layer is greatly affected by temperature, the growth temperature must be kept uniform between different rooting devices.

Further, the material of the rooting device comprises plastic or stainless steel.

Further, the nutrient substance includes soil or nutrient solution. It should be noted that in the technical scheme of the utility model, when soil is adopted, the soil is required to be uniform in size and free of stones, the surface of the root binding device is flush with the upper edge of the basket when the root binding device is loaded in the root binding device, and the upper edge of the root binding device is flush with the soil around the basket when the root binding device is buried in a paddy field; and when the nutrient solution is adopted, the nutrient solution submerges the upper edge of the root pricking device by 2-4 cm. The technical scheme of the utility model among, above-mentioned condition is incomplete prerequisite, only need guarantee it has hindering layer one side and the growth environment of no hindering layer one side is unanimous completely or tends to unanimity to prick root device.

Further, the solid culture medium comprises one or more of culture soil, fine sand, small stone blocks or glass beads.

In a preferred embodiment of the present invention, the basket with the semi-wax is made of plastic, the inner diameter of the upper edge is 17cm, the inner diameter of the lower edge is 10cm, the depth is 6.5cm, and the inner diameter of the pore space is 2 mm; the weight of the product before immersion in the wax liquid is 30g, and the weight of the product after immersion in the wax liquid is 75 g; the technical scheme of the utility model among, the even foraminiferous device of other sizes, shape and material also can be used, guarantee to have hindering layer one side and the even symmetry in non-hindering layer one side can, for example: larger or smaller size, or stainless steel material, etc., without limitation to the above preferred conditions.

In a preferred embodiment of the present invention, the purpose of the mark hole is that the root-pricking device is used for marking to ensure that the rice seedling is at the mark hole, and the planting depth requires that the seedling is 1-3 cm, preferably 2cm, in the solid culture medium.

The design principle of the utility model lies in:

the rice is prevented from rooting by the barrier layer, and the root-rooting ratio value of the side with the barrier layer and the side without the barrier layer on the same rice is calculated to be used as an index of the root-rooting capability of the plant. The identification device of the utility model has the feasible premise that the root binding device is provided with holes uniformly and has symmetrical central axis, one side of the root binding device is provided with a barrier layer and the other side is provided with no barrier layer along the symmetrical side of the central axis, the side with the barrier layer and the side without the barrier layer are symmetrical and have equal area, a certain plant is planted at the mark hole, the number of the roots which penetrate out from any two halves of the outer surface of the root binding device is the same, and the ratio of the number of the roots at the two sides of the normal basket is; when the root-pricking capacity of the plant is stronger, the number of the roots penetrating out of the side with the wax is closer to that of the side without the wax, and the ratio is closer to 1; when the plant root-pricking capacity is weaker, the number of roots which penetrate out of the side with the wax is smaller, and the ratio of the root-pricking capacity to the root-pricking capacity is closer to 0.

Compared with the prior art, the utility model discloses following beneficial effect has:

(1) the utility model discloses in the root of the same trunk material two halves of comparison, with in the past between different plants comparison or the comparison of root number about the wax layer, two halves root no matter growth state or physiology in-process all more tend to unanimity, stable, so the result is more accurate.

(2) The identification device in the prior art comprises a cylinder with the bottom sealed by a wax layer, uniform sandy soil poured on the cylinder and the like, and the identification device is complicated in process and cannot be cultivated in a large scale; the utility model discloses the device is simple, and the processing ease is fit for extensive cultivation and observes.

(3) Moisture nutrition in the jar is not smooth with external environment exchange among the prior art's identification device, is unfavorable for the normal growth of plant, the utility model discloses the device can have abundant moisture and nutrition to exchange with the environment, and vegetation state is as good as with ordinary field or nutrient solution cultivation, and the rice seedling is healthy very strong, and the result is more close true natural state.

Drawings

FIG. 1 is a schematic diagram showing the principle of identifying the root-tying ability of rice by a heavy pressing method in the prior art;

FIG. 2 is a schematic diagram of the principle of the wax layer method for identifying the root-cutting ability of rice in the prior art;

FIG. 3 is a schematic structural view of the device for identifying the root-tying ability of rice of the present invention; wherein, 1-rooting device, 2-marking hole, 3-culturing device, 4-nutrient substance and 5-solid culturing substrate;

fig. 4 is a schematic structural view of the middle circular root-pricking device of the present invention.

Detailed Description

The invention is further described in the following examples, which are intended to be illustrative only and not limiting.

Referring to fig. 3, the rice rooting capacity identification device comprises: a rooting device 1 filled with a solid culture medium 5 and a culture device 3 filled with nutrient substances 4; the root pricking device 1 is arranged in the culture device 3; wherein,

prick root device 1 even foraminiferous and be central axis symmetry, prick root device 1 and divide into two parts that symmetry and surface area equal along arbitrary well axial plane: the barrier comprises a barrier area and an unobstructed area, wherein barrier layers are uniformly distributed on the inner surface and/or the outer surface of the barrier area; a marking hole 2 is formed in the center position of the solid culture medium 5, rice seedlings are planted in the marking hole 2, and the planting depth falls into 1-3 cm of the solid culture medium 5; wherein the cross-section of the rooting device 1 comprises a circle, an ellipse or a square.

Wherein, the barrier layer is a wax layer and comprises a mixture of vaseline and paraffin, the vaseline accounts for 20-80% of the total mass of the mixture of vaseline and paraffin, the vaseline is uniformly distributed on the inner surface and/or the outer surface of the root device 1, and the thickness of the vaseline is 2-5 mm. Because the root-pricking capabilities of different rice varieties have great difference, for example, the root-pricking device with lower vaseline concentration ratio is selected for the rice variety with extremely strong root-pricking capability; the root-tying device with a higher vaseline concentration ratio is selected for rice varieties with extremely weak root-tying capability, the melting temperature is changed according to the vaseline ratio, and the higher the vaseline ratio is, the lower the temperature is. In addition, since the hardness of the barrier layer is greatly affected by temperature, the growth temperature must be kept uniform between different rooting devices.

The nutrient substances 4 comprise soil or nutrient solution, when the soil is adopted, the required size is uniform and no stones are generated, the nutrient substances are loaded into the root tying device 1, the surface of the nutrient substances is flush with the upper edge of the basket, and when the nutrient substances are buried in a paddy field, the upper edge of the root tying device 1 is flush with the surrounding soil; when the root pricking device is immersed in the nutrient solution, the nutrient solution immerses the upper edge of the root pricking device by 3cm, so that the growth environments of the side, with the barrier layer, of the root pricking device 1 and the side, without the barrier layer, are completely consistent or tend to be consistent.

In the preferred embodiment of the present invention, the rooting device 1 can be a plastic basket, as shown in fig. 4, the inner diameter of the upper edge is 17cm, the inner diameter of the lower edge is 10cm, the depth is 6.5cm, and the inner diameter of the pore space is 2 mm; the weight of the coating before dipping in the wax liquid is 30g, the weight of the coating after dipping in the wax liquid is 75g, and the side with the barrier layer and the side without the barrier layer are uniform and symmetrical.

When the root-penetrating ability of the rice is identified, the root-penetrating ability is identified according to conventional conditions such as field management of the rice and according to a conventional cultivation and production mode. The method comprises the steps of selecting full grains of IRAT109 rice seeds harvested in the same year, and soaking in water at 28 ℃ for pregermination for 2 days. Selecting seeds germinated in the white leaves, placing the seeds on a 96-hole PCR plate with the exposed bottom, placing the seeds in a constant-temperature climate box with the night temperature and the day temperature of 25/30 ℃ respectively for 14 days, and starting sowing after the seeds grow to 2 leaves and 1 heart; then, filling prepared semi-wax baskets with soil, burying the semi-wax baskets into a paddy field, punching holes in the centers of the solid culture matrixes 5 in the baskets by using a circle center puncher for marking, transplanting the seedlings to the positions of the marking holes 2, wherein the transplanting depths are uniform, and the seedling root bases fall at the bottoms of the marking holes 2 as a standard; the basket is required to be filled with uniform soil without stones, the surface of the filled soil is flush with the upper edge of the basket, the upper edge of the basket is required to be flush with soil around the basket after the soil is buried, the young seedlings are guaranteed to be in the middle of the basket, and the planting depth requires that seeds fall 2cm below the soil surface; evenly strong plantlets were picked for transplantation, 3 replicates per concentration basket.

And finally, after the rice seedlings grow for 50 days under the normal rice seedling cultivation and management conditions, the basket is firstly pulled out slightly, the number of the roots which penetrate out of the two sides of the basket with the wax and the number of the roots which do not penetrate out of the basket with the wax are counted, and the ratio of the roots to the roots is calculated to be used as an index of the root-tying capability of the material. When the number of the roots is large, the roots penetrate through the pores of the baskets with the wax half side and the roots without the wax half side respectively, the ratio between the roots and the roots is calculated finally, the baskets with each concentration are transplanted with rice seedlings for 3 times repeatedly, and the average value of the ratios of the three times is used as the final index of the root-pricking capacity of the rice, as shown in table 1.

TABLE 1 Rice rooting index

As can be seen from table 1, for IRAT109 rice cultivars, the average value of root index was about 35% in the basket with a vaseline mass ratio of 35%, the average value of root number was about 50% in the basket with a vaseline mass ratio of 50%, and the average value of root number was about 65% in the basket with a vaseline mass ratio of 65%, which indicated that the rice cultivars had the largest up-down variation space of three root numbers in the basket with a vaseline mass ratio of 50%, and the corresponding wax concentrations were most suitable for the identification of the present invention. It should be noted that the rooting ability of different rice varieties has great difference, for example, a rice variety with extremely strong rooting ability is selected to be a basket with a semi-wax having a lower vaseline concentration ratio; the rice variety with extremely weak rooting capability is characterized in that a basket with semi-wax and higher vaseline concentration ratio is selected, the melting temperature is changed according to the vaseline ratio, and the larger the vaseline ratio is, the lower the temperature is.

The above embodiments only express the embodiments of the present invention, and the description thereof is more detailed, as long as the technical personnel in the field are looking at the embodiments of the present invention, without departing from the concept of the present invention, the changes made all belong to the protection scope of the present invention.

Claims (8)

1. A rice rooting ability evaluating device, comprising: a rooting device (1) filled with a solid culture medium (5) and a culture device (3) filled with nutrient substances (4); the root pricking device (1) is arranged in the culture device (3); wherein,

prick root device (1) even foraminiferous and be the central axis symmetry, prick root device (1) and divide into two parts that symmetry and surface area equal along arbitrary well axial plane: the barrier comprises a barrier area and an unobstructed area, wherein barrier layers are uniformly distributed on the inner surface and/or the outer surface of the barrier area; a marking hole (2) is formed in the central position of the solid culture substrate (5);

and rice seedlings are planted at the mark holes (2) with the planting depth of 1-3 cm in the solid culture medium (5).

2. The rice rooting ability assessment device according to claim 1, wherein the cross-section of the rooting device (1) comprises a circle, an ellipse or a square.

3. The rice rooting ability evaluation device according to claim 1, wherein the thickness of the barrier layer of the rooting device (1) is 2 to 5 mm.

4. The rice rooting ability assessment device according to claim 3, wherein the barrier layer of the rooting device (1) is a wax layer.

5. The rice rooting ability assessment device according to claim 1, wherein the material of the rooting device (1) comprises a plastic or stainless steel material.

6. The rice rooting ability evaluation device according to claim 1, wherein the nutrient (4) comprises soil or a nutrient solution,

the soil is flush with the surface of the solid culture medium (5) in the root pricking device (1);

and the nutrient solution submerges the solid culture medium (5) in the root pricking device (1) by 2-4 cm.

7. The rice rooting ability assessment device according to claim 1, wherein the rice seedlings are planted in the marking holes (2) to a depth of 2cm within the solid culture medium (5).

8. The rice rooting ability evaluation device according to claim 1, wherein the solid culture medium (5) comprises one or a combination of two or more of culture soil, fine sand, small stone, or glass beads.