CN207908370U - The fast screening reagent box of pyrazolone ntipyretic analgesic medicine in a kind of herbal tea - Google Patents

The fast screening reagent box of pyrazolone ntipyretic analgesic medicine in a kind of herbal tea Download PDF

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Publication number
CN207908370U
CN207908370U CN201820163834.8U CN201820163834U CN207908370U CN 207908370 U CN207908370 U CN 207908370U CN 201820163834 U CN201820163834 U CN 201820163834U CN 207908370 U CN207908370 U CN 207908370U
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China
Prior art keywords
herbal tea
pyrazolone
box
diluent
analgesic medicine
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CN201820163834.8U
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Inventor
温家欣
马红圳
刘超立
何嘉雯
曹雅静
黄志业
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Shenzhen Rui Rui Biotechnology Ltd By Share Ltd
Guangdong Provincial Institute For Drug Control (guangdong Provincial Institute For Drug Quality Control And Guangdong Port Drug Control Institute)
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Shenzhen Rui Rui Biotechnology Ltd By Share Ltd
Guangdong Provincial Institute For Drug Control (guangdong Provincial Institute For Drug Quality Control And Guangdong Port Drug Control Institute)
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Abstract

The utility model discloses a kind of fast screening reagent boxes of pyrazolone ntipyretic analgesic medicine in herbal tea.Including packing box, herbal tea diluent reagent bottle, plastics measuring pipette, sampling stirring rod, 0.2mL plastic suction pipes, specimen cup with a scale are housed in the packing box;One test paper bucket has red micropore, test strips in test paper bucket.Herbal tea sample is after herbal tea dilution described in the utility model processing, it is added dropwise 7 to drop in red micropore, be aspirated 13 times up and down with 0.2mL plastic suction pipes, then test strips are inserted into red micropore, after 20 40 DEG C are reacted 6min, takes out test strips and carry out result interpretation in 3min.The utility model focuses on all medicaments that need to be used and tool in one packing box, it is no longer necessary to use external large scale equipment, carry with it is easy to use, be conducive to the fast-field evaluation of the fast screening reagent box of pyrazolone ntipyretic analgesic medicine in herbal tea.

Description

The fast screening reagent box of pyrazolone ntipyretic analgesic medicine in a kind of herbal tea
Technical field
The utility model is related to biochemical analysis detection fields, and in particular to one kind is for pyrazoles in on-site measurement herbal tea The fast screening reagent box of ketone ntipyretic analgesic medicine.
Background technology
Herbal tea is one kind of medicinal herb tea, health protection tea, is that the south of the Five Ridges people are special according to the climatic characteristic and water and soil of local rainy humidity Property, during preventing hot summer weather, disease for a long time, it is guidance with tcm health preserving theory, based on Chinese herbal medicine, develops and sum up One kind has beverage general name that is clearing heat and detoxicating, promoting the production of body fluid to quench thirst and other effects.It is generally believed that herbal tea has heat-clearing, lower fire, treatment winter Caused by dry the effect of the illness such as throat pain[1].But while herbal tea Industry Quick Development, fierce market competition Also many problems are brought.It is reported that some illegal businessmans are to heighten the effect of a treatment, chemicals are illegally added in herbal tea[2,3], may Serious consequence can be brought to drinking person.
Applicant carries out Risk Monitoring to illegally adding chemicals in herbal tea, finds there is illegal addition pyrazolone solution The case where hot analgesic.Pyrazolone ntipyretic analgesic medicine has that refrigeration function is notable, analgesic effect is strong, the spies such as cheap and easy to get Point[4], but its adverse reaction is more, if analgin can cause allergic rash, drug fever, severe patient will appear exfoliative dermatitis, Alpastic anemia[5];Aminopyrine can cause granulocyte reduction or alpastic anemia, generate carcinogenic substance nitrous Amine[6,7].Therefore, pyrazolone ntipyretic analgesic medicine is illegally added in herbal tea there are larger food safety hazards.
Currently, the research that pyrazolone ntipyretic analgesic medicine is illegally added in herbal tea has not been reported, the antipyretic town of pyrazolone The method of inspection of pain medicine mainly has high performance liquid chromatography[8], liquid chromatography tandem mass spectrometry[9], thin-layered chromatography[10], light splitting Photometry[11], capillary chromatography[12]Deng.The above large-scale instrument method is cumbersome, Check-Out Time is long, is unsatisfactory for live quick The demand of detection.
Bibliography
[1] such as Wang Ping Cantoneses herbal tea progress food science and technologies, 2010,3 (2):77-80
[2] the refined equal .RP-HPLC methods of He Wen measure in the content for illegally adding paracetamol and caffeine in herbal tea Traditional Chinese medicines thing, 2011,25 (2):126-128
[3] the quick Guangdong the detection chemical industry of paracetamol in the such as literary brightness guangdong herbal teas in bulk, 2013,40 (1) are thanked: 104-113
[4] such as Chen Wanxia analgesic-antipyretic analysis of drug use clinical rational drug uses, 2011,4 (2A):80
[5] such as Hou Chunxia brief talk the reasonable of analgesic-antipyretic and apply medicine forum of bases, 2012,16 (29):3909- 3010
[6] how the such as Hunan Hong Li correctly apply analgesic-antipyretic China community doctor, 2004,6 (10):6
[7] Wang Jing brief talk the reasonable employment contemporary Chinese medicinal applications of analgesic-antipyretic, 2010,4 (5):140
[8] .HPLC-PDA such as Hao Lihua methods measure the four kinds of antipyretic-antalgics illegally added in Radix Astragali polysaccharide injection simultaneously Class drug China veterinary drug magazine, 2012,46 (8):28-31
[9] the .LC-MS/MS methods such as Pan Wei measure 23 non-steroidal anti-inflammatory drugs Pharmaceutical Analysis magazines in Chinese patent medicine preparation, 2012,32(2):261-266
[10] the thin-layered chromatography such as Hao Lihua is quickly screened in the chemical substance illegally added in Radix Astragali polysaccharide injection State's veterinary drug magazine, 2012,46 (5):29-30
[11] the three wavelength spectrophotometries such as Zhang Keliang measure in Naoqing tablets traditional Chinese medicines in aminopyrine and content of caffeine Product standard, 2001,2 (2):35-36
[12] such as Zhao Yanfang Microemulsion Electrokinetic Chromatographies quickly analyze the active ingredient analyses in analgesic-antipyretic Laboratory, 2012,31 (4):90-93
Utility model content
In order to overcome above-mentioned technical problem, the purpose of this utility model is to provide one kind to be conducive in on-site measurement herbal tea The fast screening reagent box of pyrazolone ntipyretic analgesic medicine.
The purpose of this utility model is to provide a kind of fast screening reagent boxes of pyrazolone ntipyretic analgesic medicine in herbal tea.
Technical solution adopted in the utility model is:
The fast screening reagent box of pyrazolone ntipyretic analgesic medicine in a kind of herbal tea, including packing box, the packing box are built-in There are several herbal tea diluent reagent bottles, it is 1mL plastics measuring pipette identical with herbal tea diluent reagent bottle quantity, dilute with herbal tea Release the identical sampling stirring rod of liquid reagent bottle quantity, 0.2mL plastic suction pipes identical with herbal tea diluent reagent bottle quantity, with it is cool The identical specimen cup with a scale of tea diluent reagent bottle quantity;One test paper bucket has and herbal tea diluent reagent bottle in test paper bucket The identical red micropore of quantity, test strips identical with herbal tea diluent reagent bottle quantity.
As a further improvement of the above technical scheme, the reagent of the bottled load of herbal tea diluent reagent is Tris-PBS Buffer solution.
As a further improvement of the above technical scheme, the herbal tea diluent reagent bottle includes bottle cap and body, described The junction of bottle cap and body is equipped with sealing ring;The bottle cap is made of upper cover and lower cover.
As a further improvement of the above technical scheme, buffering bottom supporter, the buffering bottom supporter are provided in the packing box On be provided with shrinkage pool for housing herbal tea diluent reagent bottle and test paper bucket.
As a further improvement of the above technical scheme, the minimum scale of the 1mL plastics measuring pipette is 0.5mL.
As a further improvement of the above technical scheme, the herbal tea diluent reagent bottle, red micropore, test strips, band Scale specimen cup, sampling stirring rod, 1mL plastics measuring pipette, 0.2mL plastic suction pipes respectively have ten parts.
The utility model has the beneficial effects that:
(1) for fluid sample, the utility model is sampled with 1mL plastics measuring pipettes and is placed on herbal tea diluent reagent bottle In, it shakes up spare.It for solid sample, takes about 0.1g samples in specimen cup with a scale, adds water to 10mL graduation marks, with sampling Stirring rod mixing.After standing 2min, the above-mentioned sample liquids of 1mL are drawn with 1mL plastics measuring pipettes, herbal tea diluent reagent bottle is added In, it shakes up spare.Test paper bucket is opened, red micropore corresponding with sample size is taken out and is placed on red micropore grillage.It opens cool The upper cover of tea diluent reagent bottle, the vertical 7 drop solution that are added dropwise aspirate 1-3 up and down in red micropore, then with 0.2mL plastic suction pipes Secondary mixing.Test strips corresponding with sample size are taken out from test paper bucket, are inserted into red micropore, react 6min in 20-40 DEG C, Test strips are taken out from red micropore, result interpretation is carried out in 3min.Due to improving herbal tea diluent reagent, red micropore Content, the formula of test strips and ratio, and all medicaments that need to be used and tool are focused in a packing box, no longer need Will use external large scale equipment, carry with it is easy to use, be conducive to pyrazolone ntipyretic analgesic medicine in herbal tea fast sieve try The fast-field evaluation of agent box.
(2) the utility model have many advantages, such as the good, high sensitivity of specificity, it is easy to operate, field quick detection can be achieved, Supervision department can be better met, the needs that testing agency's field surveillance is enforced the law.
Description of the drawings
Fig. 1 is the structural schematic diagram of the packing box of the utility model;1 is packing box;
Fig. 2 is the schematic diagram that article is placed in the utility model packing box, wherein 2 be test paper bucket, 21 be the upper of test paper bucket Lid, 3 be herbal tea diluent reagent bottle, and 31 be the upper cover of herbal tea diluent reagent bottle, and 32 be the lower cover of herbal tea diluent reagent bottle, 4 be sampling stirring rod, and 5 be specimen cup with a scale, and 6 be 0.2mL plastic suction pipes, and 7 be 1mL plastics measuring pipettes, and 8 be buffering bottom Support, 9 be test strips, and 10 be red micropore;
Fig. 3 is the interpretation schematic diagram of testing result.
Specific implementation mode
The utility model is further described with reference to specific embodiment.
Embodiment 1
The fast screening reagent box of pyrazolone ntipyretic analgesic medicine in a kind of herbal tea as depicted in figs. 1 and 2, including leatheroid Packing box 1, packing box 1 is interior to be equipped with 12,10 bottles of test paper bucket, 3,10, herbal tea diluent reagent bottle specimen cup 5,10 with a scale Sample 4,10 1mL plastics measuring pipettes 7 of stirring rod and 10 0.2mL plastic suction pipes 6.10 test strips 9 are housed in test paper bucket 2 With 10 red micropores 10, each micropore includes the specific antigen of colloid gold label.It is equipped in herbal tea diluent reagent bottle 3 Tris-PBS buffer solutions.
Buffering bottom supporter 8 is provided in packing box 1, damage caused by avoiding reagent bottle in transportational process due to jolting is delayed Rush the shrinkage pool being provided on collet 8 for housing herbal tea diluent reagent bottle 3 and test paper bucket 2.Herbal tea diluent reagent bottle 3 includes The junction of bottle cap and body, bottle cap and body is equipped with sealing ring, prevents the leakage of liquid in transportational process.Test paper bucket 2 includes The junction of bottle cap and ladle body, bottle cap and body is equipped with sealing ring, prevents test strips 9 from making moist.Red micropore 10 has micropore plug, Prevent the specific antibody of its interior colloid gold label from making moist.
Preferably, the minimum scale of 1mL plastics measuring pipette is 0.5mL.
The method sieved soon using the kit in the implementation case is as follows:
One, sample pre-treatments
1, prepared by sample liquid:
(1) fluid sample:After sample shakes up, 1mL is drawn with 1mL plastics measuring pipettes, herbal tea diluent reagent bottle is added In, it shakes up.
(2) solid sample:It takes about 0.1g samples in specimen cup with a scale, adds water to 10mL graduation marks, stirred with sampling Stick mixing.After standing 2min, the above-mentioned sample liquids of 1mL are drawn with 1mL plastics measuring pipettes, is added in herbal tea diluent reagent bottle, shakes It is even.
Two, it detects
(1) test paper bucket is opened, red micropore corresponding with sample size is taken out.
(2) upper cover of herbal tea diluent reagent bottle is unscrewed, the vertical 7 drop sample liquids that are added dropwise are in red micropore.
(3) 0.2mL plastic suction pipes are used to aspirate 1-3 times up and down.
(4) test paper bucket is opened, test strips corresponding with sample size are taken out, is inserted into corresponding red micropore, in 20-40 DEG C reaction 6min.
(6) test strips are taken out from red micropore, carry out result interpretation.
Three, result judgement
(1) in the case that control line (C lines) normally develops the color, result judgement is carried out by below table standard:
Detection line (T lines) is compared with control line (C lines) color depth As a result judge
T lines >=C lines It is negative
T lines < C lines or T lines do not develop the color It is positive
(2) in the case that control line (C lines) does not develop the color, testing result is invalid, it is proposed that retests.Interpretation schematic diagram is as schemed Shown in 3.
Above-described embodiment is the preferable embodiment of the utility model, but the embodiment of the utility model is not by above-mentioned The limitation of embodiment, under other any Spirit Essences and principle without departing from the utility model made by change, modify, replace In generation, simplifies combination, should be equivalent substitute mode, is included within the scope of protection of the utility model.

Claims (7)

1. the fast screening reagent box of pyrazolone ntipyretic analgesic medicine in a kind of herbal tea, including packing box, it is characterised in that:The packet Several herbal tea diluent reagent bottles are housed in mounted box, 1mL plastics measuring pipette identical with herbal tea diluent reagent bottle quantity, Sampling stirring rod identical with herbal tea diluent reagent bottle quantity, 0.2mL plastics identical with herbal tea diluent reagent bottle quantity Suction pipe, specimen cup with a scale identical with herbal tea diluent reagent bottle quantity;One test paper bucket, has in test paper bucket and herbal tea dilutes The identical red micropore of liquid reagent bottle quantity, test strips identical with herbal tea diluent reagent bottle quantity.
2. the fast screening reagent box of pyrazolone ntipyretic analgesic medicine in herbal tea according to claim 1, it is characterised in that:Institute The reagent for stating the bottled load of herbal tea diluent reagent is Tris-PBS buffer solutions.
3. the fast screening reagent box of pyrazolone ntipyretic analgesic medicine in herbal tea according to claim 1, it is characterised in that:Institute It includes bottle cap and body to state herbal tea diluent reagent bottle, and the junction of the bottle cap and body is equipped with sealing ring;The bottle cap by Upper cover and lower cover composition.
4. special according to the fast screening reagent box of pyrazolone ntipyretic analgesic medicine in claims 1 to 3 any one of them herbal tea Sign is:It is provided with buffering bottom supporter in the packing box.
5. the fast screening reagent box of pyrazolone ntipyretic analgesic medicine in herbal tea according to claim 4, it is characterised in that:Institute State the shrinkage pool being provided on buffering bottom supporter for housing herbal tea diluent reagent bottle and test paper bucket.
6. the fast screening reagent box of pyrazolone ntipyretic analgesic medicine in herbal tea according to claim 1, it is characterised in that:Institute The minimum scale for stating 1mL plastics measuring pipettes is 0.5mL.
7. the fast screening reagent box of pyrazolone ntipyretic analgesic medicine in the herbal tea according to claims 1 or 2 or 3 or 5 or 6, It is characterized in that:The herbal tea diluent reagent bottle, red micropore, test strips, specimen cup with a scale, sampling stirring rod, 1mL modelings Material measuring pipette, 0.2mL plastic suction pipes respectively have ten parts.
CN201820163834.8U 2018-01-30 2018-01-30 The fast screening reagent box of pyrazolone ntipyretic analgesic medicine in a kind of herbal tea Active CN207908370U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112461825A (en) * 2020-11-10 2021-03-09 苏州慧源安食检测科技有限公司 Rapid detection method for rapidly and quantitatively detecting carbendazim pesticide

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112461825A (en) * 2020-11-10 2021-03-09 苏州慧源安食检测科技有限公司 Rapid detection method for rapidly and quantitatively detecting carbendazim pesticide

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