CN207877746U - A kind of micro-fluidic chip for unicellular capture and culture - Google Patents
A kind of micro-fluidic chip for unicellular capture and culture Download PDFInfo
- Publication number
- CN207877746U CN207877746U CN201820002900.3U CN201820002900U CN207877746U CN 207877746 U CN207877746 U CN 207877746U CN 201820002900 U CN201820002900 U CN 201820002900U CN 207877746 U CN207877746 U CN 207877746U
- Authority
- CN
- China
- Prior art keywords
- micro
- block
- fluidic chip
- micropore
- array
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 238000009826 distribution Methods 0.000 claims abstract description 4
- 239000012530 fluid Substances 0.000 claims description 10
- 238000003491 array Methods 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 12
- 239000007853 buffer solution Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 239000004205 dimethyl polysiloxane Substances 0.000 description 3
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 3
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 3
- 238000001259 photo etching Methods 0.000 description 3
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 3
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000002174 soft lithography Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007821 culture assay Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses it is a kind of for it is unicellular capture and culture micro-fluidic chip, including combine upper piece and bottom sheet, it is described upper piece and the bottom sheet apparent surface on be respectively arranged with block array and microwell array；The block of the block array correspondingly separates distribution with the micropore of the microwell array, and it is configured to, the block captures the individual cells in positive liquid stream and the cell in positive liquid stream is prevented to enter its straight-ahead micropore, and the cell that the block is captured is gone out the block using reversed liquid stream and flows into the micropore in its reversed front；The block array is located in the microchannel of upper piece surface formation, and the microchannel has entrance and exit on the micro-fluidic chip.The utility model can realize to simple and effective single celled capture, array arrangement and culture.
Description
Technical field
The utility model is related to a kind of micro-fluidic chips for unicellular capture and culture.
Background technology
Single cell analysis have been developed as cell biology research cell function a critical issue, and by
It is limited to tool and technical support makes separation to individual cells, the problems such as subsequent unicellular research is become increasingly to dash forward
Go out.The single celled mode of tradition extraction is complicated, time-consuming, laborious and efficiency is bad.And manipulated using micro-fluidic chip it is unicellular, only
It needs fluid being passed through chip, then carries out certain operation and can be obtained experimental result.This needs a few minutes that can complete,
Conventional efficient can be improved so that experiment is rapider, convenient.It, then can be same to unicellular carry out array on micro-fluidic chip
The multigroup control experiments of Shi Jinhang, substantially increase conventional efficient.
Microfluidic chip technology is a kind of low reynolds number flow using fluid under micro-meter scale as the science of main feature
Technology is that a variety of monotechnicses flexible combination and scale in small controllable platform are integrated.We can be at one piece several squares lis
On the micro-fluidic chip of rice, complicated microchannel network is constructed, and to fluid therein accurately manipulate and control.This
Different modules is integrated on a small-sized platform by kind chip, provides great convenience.
Currently, there have the technology much based on block array that can be manipulated by micro-fluidic chip to be unicellular.Texas,U.S
Researcher is captured using block and wall to unicellular, and makes cell adherence in substrate, then removes block and wall
Wall, to make cell be deposited on micro-fluidic chip.The pull-in time of this method is longer, and can not be effective with cell culture
It is combined together.California, USA researcher is captured using monomer block array to unicellular, but it is relatively low to capture success rate；
And capture need 24 hours every time, it is less efficient.
Utility model content
The main purpose of the utility model is that in view of the deficiencies of the prior art, providing a kind of for unicellular capture and training
Foster micro-fluidic chip.
To achieve the above object, the utility model uses following technical scheme：
It is a kind of for it is unicellular capture and culture micro-fluidic chip, including combine upper piece and bottom sheet, it is described
Upper piece and the bottom sheet apparent surface on be respectively arranged with block array and microwell array；The block of the block array and institute
The micropore for stating microwell array correspondingly separates distribution, and is configured to, and the block captures the individual cells in positive liquid stream simultaneously
The cell in positive liquid stream is prevented to enter its straight-ahead micropore, and reversed liquid stream goes out the cell that the block is captured
The block and the micropore for flowing into its reversed front；The miniflow that the block array is located at upper piece surface formation is logical
In road, the microchannel has entrance and exit on the micro-fluidic chip.
Further：
One microchannel connects multiple block arrays to form a fluid passage or a plurality of described micro-
Multiple block array in parallel are formed a plurality of fluid passage by circulation road.
The block is made of axisymmetric two plate portions, and described two plate portions are straight-ahead close to its
The side of micropore has larger gap, has smaller gap in the side far from its straight-ahead micropore, described larger
Gap be more than target cell diameter, the smaller gap be less than target cell diameter.
Described two plate portions are in 90 degree.
The symmetry axis of the block is parallel with flowing to for the micro-fluidic chip.
The larger gap is equal with the diameter of the micropore；The smaller gap is the 0.4 of target cell diameter
- 0.8 times, preferably 0.6 times again.
Height of the block in the microchannel be 1.2 times -2 times of target cell diameter, preferably 1.5 times；It is preferred that
Ground, the deep equality of the height of the block and the microchannel.
The depth of the micropore be 2 times -5 times of target cell diameter, preferably 3 times.
The liquid inlet of the micro-fluidic chip and liquid outlet are connected with the syringe pump for driving liquid to flow.
The micro-fluidic chip by upper piece and bottom sheet be bonded together it is obtained.
The utility model has the advantages that：
Micro-fluidic chip provided by the utility model accurately can quickly be captured unicellular from a large amount of cells, Gu
It is fixed to be arranged in array to specified position, it is used for single celled analysis.Using the micro-fluidic chip of the utility model, can incite somebody to action
Unicellular capture, array, culture are integrated on single micro-fluidic chip, can implement unicellular high throughput by shirtsleeve operation
Acquisition and culture assays.
Micro-fluidic chip provided by the utility model for unicellular capture and culture overcomes current published skill
Unicellular capture is low with cell survival rate existing for culture technique in art, catch rate is low, success rate is low, is difficult to realize high throughput catches
The deficiencies of catching, the micro-fluidic chip of the utility model utilize fluid mechanics principle and microflow control technique, by flow focusing theory with
Capturing structure is combined, and is matched with microwell plate, realizes unicellular accurate capture and culture, has high catch rate, high success
The characteristics of rate, high throughput, strong applicability, and it is processing simple in structure, easy to operate, at low cost, and then as disclosure satisfy that scientific research
With the tool of clinical demand, development mechanism, diagnosis and treatment etc. are provided for unicellular research, provides new research and experiment hand
Section.
The utility model has the following advantages：
1. easily realizing unicellular efficient capture, arranging and cultivate to array；
2. it makes simply, it is at low cost；
3. it is high-throughput, multigroup control experiment can be completed at the same time；
4. structure is captured conducive to cell, capturing efficiency is high；
5. cell survival rate is high.
Description of the drawings
Fig. 1 be the utility model embodiment micro-fluidic chip upper piece and bottom sheet combination diagram；
Fig. 2 is upper piece the upward view in the utility model embodiment micro-fluidic chip；
Fig. 3 is the perspective and partial enlarged view of the utility model embodiment micro-fluidic chip；
Fig. 4 is the single capturing structure schematic diagram of the utility model embodiment micro-fluidic chip；
Fig. 5 is that the utility model embodiment micro-fluidic chip captures and the flow diagram of culture, in figure from top to bottom according to
Secondary to be rinsed for runner, cell capture, cell rinses, back flush capture, five steps of cell culture.
Reference sign：
Upper of 1-；2- bottom sheet；The liquid inlets 3-；4- liquid outlets；5- micropores；6- microchannels；7- blocks；8- targets
Cell.
Specific implementation mode
It elaborates below to the embodiment of the utility model.It is emphasized that following the description is only example
Property, rather than in order to limit the scope of the utility model and its application.
Refering to fig. 1 to Fig. 5, in one embodiment, a kind of micro-fluidic chip for unicellular capture and culture, including
Combine upper piece 1 and bottom sheet 2, it is described upper piece 1 and the bottom sheet 2 apparent surface on be respectively arranged with block array
And microwell array；The block 7 of the block array correspondingly separates distribution with the micropore 5 of the microwell array, and is configured to,
The block 7 can capture the individual cells in positive liquid stream and prevent the cell in positive liquid stream straight-ahead micro- into its
Hole 5 (is its straight-ahead micropore shown in the micropore 5 in 7 left side of block in Fig. 4, positive liquid stream is from the right side in Fig. 3-Fig. 5
The flow direction turned left), and the cell that the block 7 is captured then is gone out the block 7 by reversed liquid stream and flows into its reversed front
Micropore (the unshowned micropore on the right side of block 7 in Fig. 4 is the micropore in its reversed front, and reversed liquid stream is in Fig. 3-Fig. 5
Flow direction from left to right)；The block 7 on upper piece 1 surface it is described upper piece 1 surface and be unified into block array, by described
The microchannel 6 on upper piece 1 surface be connected into a fluid passage or and be unified into a plurality of fluid passage, every fluid passage is in institute
Stating tool on micro-fluidic chip, there are one liquid inlet 3 and a liquid outlets 4.
In a preferred embodiment, the block 7 is made of axisymmetric two plate portions, described two plate portions
There is larger gap close to the side of its straight-ahead micropore 5, having in the side far from its straight-ahead micropore 5
Smaller gap, the larger gap are more than the diameter of target cell, and the smaller gap is less than the diameter of target cell.
In a more preferred embodiment, described two plate portions are in 90 degree.
In a preferred embodiment, it is described upper piece 1 block 7 symmetry axis it is parallel with flowing to for the micro-fluidic chip.
In a preferred embodiment, the larger gap is equal with the diameter of the micropore 5；The smaller gap is
0.4 times -0.8 times of 8 diameter of target cell, most preferably 0.6 times.
In a preferred embodiment, upper piece 1 barrier height that is formed in microchannel of block 7 is target cell
8 1.2 times -2 times of diameters, most preferably 1.5 times.The height of the block 7 can be arranged to the depth phase with the microchannel 6
Deng.
In a preferred embodiment, the depth of the micropore 5 of the bottom sheet 2 is 2 times -5 times of 8 diameter of target cell, most preferably
It is 3 times.
In a preferred embodiment, the micro-fluidic chip is gathered around there are one liquid inlet 3 and a liquid outlet 4, this two
A liquid inlet 3 and liquid outlet 4 through it is entire upper piece 1, and be connected with upper piece 1 microchannel 6.
In a preferred embodiment, the liquid inlet 3 of the micro-fluidic chip and liquid outlet 4 are connected with for driving liquid
The syringe pump of body flowing.
In a preferred embodiment, the micro-fluidic chip upper piece 1 and bottom sheet 2 two dimensional model is carried out by AutoCAD
It draws, by graphic making on mask plate, SU-8 structures is copied by photoetching technique, PDMS is copied to by soft lithography
On.
In a preferred embodiment, the micro-fluidic chip by upper piece 1 and bottom sheet 2 be bonded together it is obtained.
Since chip manufacturing cost is relatively low, and the cost for cleaning chip is higher, and the utility model uses jettisonable miniflow
Control chip.
Some embodiments according to the present utility model, chip are bonded with bottom sheet by upper piece and are formed.It will be micro- using photoetching technique
The structures such as circulation road, block, micropore are engraved in the SU-8 structures of silicon base.Runner includes liquid inlet, sprue and liquid
Outlet.
The all sames such as the stop block structure size of chip upper piece, spacing, the size of block array, spacing also all same.Bottom sheet
Micropore and upper piece stop block structure correspond, the liquid positioned at stop block structure flows to direction.
Chip size in a specific example of the utility model：50*60mm2；Bottom sheet micropore：200 μm of diameter, depth
50 μm of degree；Upper piece microchannel：25 μm of depth；Upper piece block：Spacing 200mm；Upper piece block array：Spacing 5mm.
Microchannel can be concatenated single channel, can also be parallel arrangement of plurality of passages.
The width of the microchannel of the micro-fluidic chip of the utility model specific embodiment is determined by the array quantity of block
It is fixed.Those skilled in the art can be with the size of designed, designed microchannel.
Syringe pump connects liquid inlet and the liquid outlet of the micro-fluidic chip, for driving liquid to flow.
It is a kind of to capture single celled method refering to Fig. 5, carry out unicellular capture, the party using the micro-fluidic chip
Method includes：
Cell capture stage, forward direction are injected in Cell Buffer to microchannel 6, and individual cells 8 are stuck between block 7
In gap, the straight-ahead micropore 5 of block 7 is also bypassed while remaining cell bypasses block 7；
Cell rinse stage, forward direction injection buffer solution wash away microchannel 6, cell remaining in microchannel 6 are rinsed
Totally, captured unicellular 8 are left behind；
Back flush acquisition phase, inverse injection buffer solution wash away microchannel 6, and cell 8 is made to leave block 7 and flow to gear
In the micropore in the reversed front of block.
It wherein wraps after celliferous buffer solution enters microchannel 6 from liquid inlet, part cell is captured by block 7.
After the completion of to be captured, the remaining cell in microchannel 6 is rinsed well, then reversed slowly logical wash buffer, makes capture
To cell fall into the micropore 5 of bottom sheet.Using the micro-fluidic chip, can realize to simple and effective it is single celled capture,
Arranges and cultivate to array；And the facture of microchip is simple for process.
During the work time, it after cell is captured by block, can be obviously reduced by the flow in block gap, therefore even if
Two cells flow to block gap, also due to unstable and along block the side of status flows away, therefore each block
A cell only can be captured.When back flush, since the streamline of liquid is substantially along the symmetrical axial symmetry of block, it is captured
Cell can be flowed towards corresponding micropore along the direction of the symmetry axis of block；When cell is flow to above micropore, by gravity
It is influenced hence into micropore with streamline.
In a preferred embodiment, movement-less part in chip, all structures are made by soft light carving technology；Upper piece and
It is bonded between bottom sheet, ensure that the leakproofness of pipeline.Chip structure is simple and efficient using process, and operation is succinct.
The Hela cells that micro-fluidic chip using the utility model is about 15 μm to average diameter carry out capture array；It is logical
It crosses AutoCAD progress two dimensional model draftings and SU-8 structures is copied by photoetching technique by graphic making on mask plate, lead to
It crosses soft lithography to copy on PDMS, to obtain micro-fluidic chip as shown in Figure 3.Microchannel 6 is located at upper piece 1, upper piece 1
Material with bottom sheet 2 is PDMS, upper piece 1 and bottom sheet 2 be bonded together using aligning bonding head of bonder, the depth of microchannel 6 is
25 μm, the depth of micropore 5 is 50 μm, a diameter of 200 μm.The gap of block is 10 μm, and the distance between block is 200 μm.
Its work flow diagram is as shown in Figure 5.Runner rinse stage is injected buffer solution by liquid inlet with syringe pump and is washed away
Microchannel, flow are 500 μ l/min, it is ensured that no visible bubble retains in microchannel.The cell capture stage is used
Syringe pump injects higher (100/μ l) Cell Buffer of density by liquid inlet, and flow is 100 μ l/min；Cell is slow
When fliud flushing flow to block, most cells can bypass block, and only a few cell can flow to block gap, and be stuck in gap,
To captured.Cell rinse stage injects buffer solution by liquid inlet with syringe pump and washes away microchannel, and flow is 100 μ
L/min rinses cell remaining in microchannel well, leaves behind captured unicellular.Back flush acquisition phase is used
Syringe pump injects buffer solution by liquid outlet and washes away microchannel, and flow is 80 μ l/min, and cell leaves block, flows to micro-
Hole.The cell culture stage is fallen into micropore by gravity when cell flows through micropore, and sinks to bottom hole, unicellular to realize
It captures, carries out follow-up cultivation work.Whole flow process can be completed in 30 seconds.
The micro-fluidic chip of the utility model also project is come real by adjusting block size, microchannel height size
Now to the capture of different-diameter target cell, the diameter and spacing that can adjust microwell array cultivate rings to the difference needed for cell
Border is adjusted.Liquid outlet end connects retracting device, and the Cell Buffer not being captured is recycled, for next time real
It tests, to reduce the waste of cell.
The utility model is based on realizing unicellular capture, array and culture using micro-fluidic chip, improves existing slender
Born of the same parents capture the problems such as of high cost, efficiency is low, success rate is low existing for spraying technique, and reach following technical indicator：
1. Manipulation of single cells system is made using micro-electromechanical processing technology, unicellular capture, array and culture are realized；
2. high-throughput：Can capture within every 30 seconds 400 it is unicellular；
3. capturing efficiency：It can complete within 30 seconds once to capture；
4. capturing success rate：But Cell capture success rate can reach 90% or more；
5. cell survival rate：95% or more can be reached by capturing cell survival rate after array is completed 12 hours.
The above content is specific/preferred embodiment further detailed description of the utility model is combined, no
It can assert that the specific implementation of the utility model is confined to these explanations.For the common skill of the utility model technical field
For art personnel, without departing from the concept of the premise utility, the embodiment that these have been described can also be made
Some replacements or modification, and these are substituted or variant all shall be regarded as belonging to the scope of protection of the utility model.
Claims (10)
1. it is a kind of for it is unicellular capture and culture micro-fluidic chip, which is characterized in that including combine upper piece and
Bottom sheet, it is described upper piece and the bottom sheet apparent surface on be respectively arranged with block array and microwell array；The block array
Block correspondingly separate distribution with the micropore of the microwell array, and be configured to, the block captures in positive liquid stream
Individual cells simultaneously prevent the cell in positive liquid stream from entering its straight-ahead micropore, and reversed liquid stream is captured the block
Cell go out the block and flow into the micropore in its reversed front；The block array is located at upper piece the surface shape
At microchannel in, the microchannel on the micro-fluidic chip have entrance and exit.
2. micro-fluidic chip as described in claim 1, which is characterized in that a microchannel is by multiple block battle arrays
Row series connection forms a fluid passage or multiple block array in parallel are formed a plurality of liquid by a plurality of microchannel
Channel.
3. micro-fluidic chip as described in claim 1, which is characterized in that the block is grouped by axisymmetric two plate-like portions
There is larger gap close to the side of its straight-ahead micropore at, described two plate portions, far from it just forward
There is smaller gap, the larger gap to be more than the diameter of target cell, the smaller gap for the side of the micropore of side
Less than the diameter of target cell.
4. micro-fluidic chip as claimed in claim 3, which is characterized in that described two plate portions are in an angle of 90 degrees.
5. micro-fluidic chip as claimed in claim 3, which is characterized in that the symmetry axis of the block and the micro-fluidic chip
Flow to it is parallel.
6. micro-fluidic chip as claimed in claim 3, which is characterized in that the diameter phase in the larger gap and the micropore
Deng；The smaller gap is 0.4 times -0.8 times of target cell diameter.
7. such as claim 1 to 6 any one of them micro-fluidic chip, which is characterized in that the block is in the microchannel
In height be 1.2 times -2 times of target cell diameter.
8. such as claim 1 to 6 any one of them micro-fluidic chip, which is characterized in that the depth of the micropore is that target is thin
2 times -5 times of born of the same parents' diameter.
9. such as claim 1 to 6 any one of them micro-fluidic chip, which is characterized in that the liquid of the micro-fluidic chip enters
Mouth and liquid outlet are connected with the syringe pump for driving liquid to flow.
10. such as claim 1 to 6 any one of them micro-fluidic chip, which is characterized in that the micro-fluidic chip by upper piece and
Bottom sheet is bonded together obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820002900.3U CN207877746U (en) | 2018-01-02 | 2018-01-02 | A kind of micro-fluidic chip for unicellular capture and culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820002900.3U CN207877746U (en) | 2018-01-02 | 2018-01-02 | A kind of micro-fluidic chip for unicellular capture and culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207877746U true CN207877746U (en) | 2018-09-18 |
Family
ID=63506342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820002900.3U Expired - Fee Related CN207877746U (en) | 2018-01-02 | 2018-01-02 | A kind of micro-fluidic chip for unicellular capture and culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207877746U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108034586A (en) * | 2018-01-02 | 2018-05-15 | 清华大学深圳研究生院 | A kind of micro-fluidic chip for unicellular seizure and culture |
| CN110305788A (en) * | 2019-05-31 | 2019-10-08 | 西安医学院 | The array chip and its preparation and operating method of cell capture and the culture of tumour ball |
| CN110687188A (en) * | 2019-09-29 | 2020-01-14 | 东北大学 | Micro-fluidic chip mass spectrometry system for single cell analysis and application method thereof |
-
2018
- 2018-01-02 CN CN201820002900.3U patent/CN207877746U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108034586A (en) * | 2018-01-02 | 2018-05-15 | 清华大学深圳研究生院 | A kind of micro-fluidic chip for unicellular seizure and culture |
| CN108034586B (en) * | 2018-01-02 | 2023-09-22 | 清华大学深圳研究生院 | Microfluidic chip for single cell capturing and culturing |
| CN110305788A (en) * | 2019-05-31 | 2019-10-08 | 西安医学院 | The array chip and its preparation and operating method of cell capture and the culture of tumour ball |
| CN110687188A (en) * | 2019-09-29 | 2020-01-14 | 东北大学 | Micro-fluidic chip mass spectrometry system for single cell analysis and application method thereof |
| CN110687188B (en) * | 2019-09-29 | 2021-05-18 | 东北大学 | Micro-fluidic chip mass spectrometry system for single cell analysis and application method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108034586A (en) | A kind of micro-fluidic chip for unicellular seizure and culture | |
| CN106497786B (en) | A kind of micro-fluidic chip for unicellular capture and culture | |
| CN207877746U (en) | A kind of micro-fluidic chip for unicellular capture and culture | |
| CN103191791B (en) | Integrated chip system for high-throughput sorting and counting detection of biological particles, and application | |
| CN106434302B (en) | A kind of micro-current controlled cell separating chips in Portable no-power source | |
| CN109456879B (en) | Dielectrophoresis micro-fluidic chip for cell sorting and focusing and alignment-free micro-processing method thereof | |
| CN103464229B (en) | Multilevel sorting microfluidic device for rare cells | |
| CN110305788A (en) | The array chip and its preparation and operating method of cell capture and the culture of tumour ball | |
| CN102695804B (en) | Methods and apparatus for segregation of particles, including segregation and proliferation of fetal and stem cells | |
| CN110628568A (en) | Slide rail type dielectrophoresis electrode structure for high-throughput continuous flow cell separation | |
| CN111744565A (en) | Microfluidic device and system for multi-channel parallel detection of cell deformability | |
| CN103923816B (en) | A kind of cell capture array based on microflow control technique | |
| CN104549587A (en) | Three-channel microsphere screening chip and use method | |
| CN207366327U (en) | A kind of whole blood blood plasma piece-rate system | |
| JP6839173B2 (en) | Fluid Mechanics Shutling Chip Equipment and Methods for Capturing Separated Single Cells | |
| CN210765350U (en) | Array micro-control chip for single cell capture and tumor ball culture | |
| Chen et al. | Insights into a deterministic lateral displacement sorting chip with new cross-section micropillars | |
| CN204891906U (en) | A micro -fluidic chip for cellular level drug screening | |
| CN106190832A (en) | There is the multiple magnetic activation separating structure micro-fluidic chip that high-purity cell reclaims | |
| CN112980677A (en) | Micro-fluidic chip for analyzing and sorting tumor cell migration capacity and preparation process | |
| CN109077004A (en) | A kind of fish constellation effect traces back the pilot system of locus model with fish | |
| KR20110064549A (en) | A microfluidic device for cultivating cells, and a method for cultivating cells using the same | |
| CN217140437U (en) | High-stability liquid drop sorting system and micro-fluidic chip comprising same | |
| KR20140142097A (en) | Method of recollecting target material | |
| KR101416634B1 (en) | Microfluidic Chip, Fabricating Method Thereof And Microfluidic Separation System |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180918 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |