CN207689421U - A kind of protein vertical slab electrophoresis gel making device - Google Patents

Abstract

The utility model provides a kind of protein vertical slab electrophoresis gel making device, stainless steel edge is added by the lower edge in glass plate, left and right edges add fixed frosted glass edge strip respectively, after another piece of glass combination, lower end, with standoff big clamp lower edge, and is fixed on experimental bench with two；Left and right ends use smaller clamp, prevent the glue for filling from being overflowed from left and right or lower edge.The stripping adhesive dispenser substantially reduces the probability for leading to the failure of an experiment because of leak adhesive, and the apparatus structure is simple, and production cost is low, can reuse.

Description

A kind of protein vertical slab electrophoresis gel making device

Technical field

The utility model is related to a kind of protein vertical slab electrophoresis gel making devices, specifically a kind of to be suitable for protein Before general electrophoresis, for making the device of offset plate.

Background technology

Polyacrylamide gel electrophoresis can be used as lipidated protein identification, protein molecular weight measures, quantification of protein, separately Glue protein isolate matter is also run in protein Vertial electrophorestic tank before outer Western Blot transferring films.Polyacrylamide gel electricity Offset plate is expeditiously made before swimming faces three problems.1, the existing gel maker lower end in market is without sealing strip, but thicker with one Rubber pad seal, form a unilateral side on be open sandwich folder shape.This device comes for being just the new hand of electrophoresis It says, leak adhesive is most commonly seen and stubborn problem can not carry out subsequent experimental as long as offset plate is done well；2, the system in laboratory Glue device is due to being used for a long time, and lower end rubber groove aging deformation, though lower end is sealed using agar, " leakage often occurs in lower end Glue " or " leakage " phenomenon；When the agar excess 3, being added in the rubber groove of lower end, agar can overflow between glass plate, account for Prepare space according to gel, cause made of gel width it is inadequate or edge is irregular.

Invention content

In view of the deficiencies in the prior art, the utility model provides a kind of protein vertical slab electrophoresis gel making device, uses Sealing edge strip of the frosted glass as the left and right sides, sealing edge strip of the stainless steel as lower end.Left and right and lower end stainless steel clip It clamps, prevents " leak adhesive " or " leakage ", be also prevented from sealing agar and overflowed into along glass gap.

In order to achieve the above object, the utility model uses the following technical solution：

A kind of protein vertical slab electrophoresis gel making device comprising：1 and second glass plate 2 of smooth first glass plate, first Glass plate 1 is a smooth glass plate, and 2 both sides of the second glass plate are equipped with frosted glass strip of paper used for sealing, and 2 bottom edge of the second glass plate is additionally provided with stainless Steel sealing strip；Small binder clip can clamp the both sides of the first glass plate 1 and the second glass plate 2, and big binder clip can clamp first The bottom of glass plate 1 and the second glass plate 2.

Preferably, 1 to the second glass plate of the first glass plate, the 2 small 1-2cm of width.

Preferably, frosted glass strip of paper used for sealing and the second glass plate 2 are bonded using fixed, stainless steel sealing strip and the second glass plate 2 Also it is bonded using fixed, lower section can realize that gel is conductive and uses for a long time stainless using stainless steel strip in electrophoresis Erosion.

Preferably, the small binder clip and big binder clip are all made of stainless steel and are made.Wherein big binder clip 7 carries holder, It can ensure that two blocks of glass are combined closely and holding is horizontal, small binder clip length 2-4.5cm, big binder clip length 4-8cm.

Preferably, the quantity of the big binder clip is more than 2, and the gel making device is made to be unlikely to topple over.

The beneficial effects of the utility model：A kind of protein vertical slab electrophoresis gel making device of the utility model, by glass The lower edge of glass plate adds stainless steel edge, and left and right edges add fixed frosted glass edge strip respectively, with another piece of glass combination Afterwards, lower end and is fixed on experimental bench with one with standoff big clamp lower edge；Left and right ends use smaller Clamp prevents the glue for filling from being overflowed from left and right or lower edge, substantially reduces the probability for leading to the failure of an experiment because of leak adhesive； In addition, the stainless steel edge of lower edge can conduction so that package unit is can be directly used for electrophoresis.The stripping adhesive dispenser is simple and practical, Production cost is low, can recycle.

Description of the drawings

Fig. 1 is a kind of 1 graphical representation of exemplary of the first glass plate of protein vertical slab electrophoresis gel making device of the utility model.

Fig. 2 is a kind of 2 graphical representation of exemplary of the second glass plate of protein vertical slab electrophoresis gel making device of the utility model.

Fig. 3 is a kind of small binder clip plan view of protein vertical slab electrophoresis gel making device of the utility model.

Fig. 4 is a kind of big binder clip plan view of protein vertical slab electrophoresis gel making device of the utility model.

Fig. 5 is a kind of protein vertical slab electrophoresis gel making device structural schematic diagram of the utility model.

Specific implementation mode

To keep the purpose of this utility model, technical solution and advantage clearer, below in conjunction with the accompanying drawings to the utility model Specific implementation mode be described in detail.The example of these preferred embodiments is illustrated in the accompanying drawings.Institute in attached drawing The embodiment of the utility model for showing and describing with reference to the accompanying drawings is only exemplary, and the utility model is not limited to this A little embodiments.

Here, it should also be noted that, in order to avoid having obscured the present invention because of unnecessary details, in the accompanying drawings only It shows the structure and/or processing step closely related with scheme according to the present utility model, and little its of relationship is omitted His details.

Embodiment 1

A kind of protein vertical slab electrophoresis gel making device is present embodiments provided, as shown in Figs. 1-5 comprising：First glass Glass plate 1 and the second glass plate 2, the first glass plate 1 are a smooth glass plate, and 2 both sides of the second glass plate are equipped with 3 He of frosted glass strip of paper used for sealing 4,2 bottom edge of the second glass plate is additionally provided with stainless steel sealing strip 5；Small binder clip 6 can clamp the first glass plate 1 and the second glass plate The big binder clip in 2 both sides 7 can clamp the bottom of the first glass plate 1 and the second glass plate 2.

Further, 1 to the second glass plate of the first glass plate, the 2 small 1-2cm of width.

Further, frosted glass strip of paper used for sealing 3 and 4 and second glass plate 2 bonded using fixed, stainless steel sealing strip 5 and second Glass plate 2 is also bonded using fixed.

Further, the small binder clip 6 and big binder clip 7 are all made of stainless steel and are made.Wherein big binder clip 7 is with branch Frame, it is ensured that two blocks of glass are combined closely and holding is horizontal, small binder clip 6 length 2-4.5cm, 7 length 4- of big binder clip 8cm。

Further, the quantity of the big binder clip 7 is more than 2, and the gel making device is made to be unlikely to topple over.

Further, as shown in Fig. 2, carry at left and right sides of the glass plate of sealing strip is frosted glass material, lower end sealing Item is stainless steel, and the two junction organically combines, cannot have the gap.

Further, as shown in Fig. 5, the side for having sealing strip on the glass with sealing strip direction is internal, two pieces Glass is combined, and sealing strip is clipped between two blocks of glass, and both sides are clamped with small binder clip, and lower end is clamped with big binder clip, and Two blocks of glass are fixed with holder included thereon.Assembling is simple, can two blocks of glass directly be injected in gel fluid injection after assembling Crack in, the step of eliminating agar back cover end glue groove, while effectivelying prevent " leak adhesive " or " leakage ", be also prevented from sealing fine jade Fat is overflowed into along glass gap.

Embodiment 2

A kind of protein vertical slab electrophoresis glue production method is present embodiments provided, is included the following steps：

1, the first glass plate 1 and the second glass plate 2 are cleaned up, both hands catch the edge of two pieces of glass plates respectively, will The two is combined, and sealing strip is made to be clipped between two pieces of glass plates, edge alignment.

2, a hand tightly pinches the first glass plate 1 and 2 simultaneously, prevents its opposite sliding；Another hand is distinguished with small binder clip 6 Its right and left is clamped.

3, big binder clip 7 is placed in experimental bench, keeps its holding horizontal by holder, opens it, the first glass plate 1 and 2 It is clipped in the middle simultaneously, you can carry out follow-up encapsulating operation.

4, to be poured into gel reservoir be polymerized to gel after, small binder clip 6 and big binder clip 7 are taken off, a hand The first glass plate 1 and 2 tightly is pinched, electrophoresis tank is put into togerther together with gel；Subsequent operation can be carried out after fixation.

In conclusion a kind of protein vertical slab electrophoresis gel making device of the utility model, passes through the lower edge in glass plate In addition stainless steel edge, after left and right edges add fixed frosted glass edge strip, with another piece of glass combination respectively, lower end is with one With standoff big clamp lower edge, and it is fixed on experimental bench；Left and right ends use smaller clamp, prevent It fills the glue entered to overflow from left and right or lower edge, substantially reduces the probability for leading to the failure of an experiment because of leak adhesive.

The above is only the specific implementation mode of the application, it is noted that for the ordinary skill people of the art For member, under the premise of not departing from the application principle, several improvements and modifications can also be made, these improvements and modifications are also answered It is considered as the protection domain of the application.

Claims (5)

1. a kind of protein vertical slab electrophoresis gel making device, which is characterized in that including：Smooth first glass plate（1）And glass plate （2）, the first glass plate（1）For a smooth glass plate, the second glass plate（2）Both sides are equipped with frosted glass strip of paper used for sealing, glass plate（2）Bottom edge It is additionally provided with stainless steel sealing strip；Small binder clip can clamp the first glass plate（1）With the second glass plate（2）Both sides, big long-tail Folder can clamp the first glass plate（1）With the second glass plate（2）Bottom.

2. protein vertical slab electrophoresis gel making device according to claim 1, which is characterized in that first glass plate （1）Than the second glass plate（2）The small 1-2cm of width.

3. protein vertical slab electrophoresis gel making device according to claim 1, which is characterized in that the frosted glass strip of paper used for sealing with Second glass plate（2）It is bonded using fixed, stainless steel sealing strip and the second glass plate（2）Also it is bonded using fixed.

4. a kind of protein vertical slab electrophoresis gel making device according to claim 1, which is characterized in that the small binder clip Stainless steel is all made of with big binder clip to be made, wherein big binder clip（7）With holder.

5. a kind of protein vertical slab electrophoresis gel making device according to claim 1, which is characterized in that the small binder clip Length is 2-4.5cm, and big binder clip length is 4-8cm.