CN207685305U - Suspension culture device - Google Patents

Suspension culture device Download PDF

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Publication number
CN207685305U
CN207685305U CN201721722873.9U CN201721722873U CN207685305U CN 207685305 U CN207685305 U CN 207685305U CN 201721722873 U CN201721722873 U CN 201721722873U CN 207685305 U CN207685305 U CN 207685305U
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CN
China
Prior art keywords
culture tank
solenoid valve
culture
mounting base
woven hose
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Active
Application number
CN201721722873.9U
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Chinese (zh)
Inventor
江泉
李超
马晓静
肖泽玺
祝加和
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Yinhai Shanghai Saint Biotechnology Co Ltd
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Yinhai Shanghai Saint Biotechnology Co Ltd
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Priority to CN201721722873.9U priority Critical patent/CN207685305U/en
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Publication of CN207685305U publication Critical patent/CN207685305U/en
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Abstract

The utility model discloses suspension culture devices,Including mounting base and culture tank,The top of culture tank is equipped with sealing cover,The side of culture tank is equipped with the woven hose communicated therewith,The end of woven hose is connected with nutrient solution and provides device,The first solenoid valve and fluid flowmeter are provided in woven hose,The air inlet pipe being connected to culture tank is provided with below woven hose,Aspiration pump is installed in air inlet pipe,Second solenoid valve and air flow meter are provided in air inlet pipe,The other side of culture tank is provided with the exhaust pipe communicated therewith,Third solenoid valve is provided in exhaust pipe,The lower section of exhaust pipe is equipped with the sewer pipe being connected to culture tank,4th solenoid valve is installed in sewer pipe,The agitating shaft extending into culture tank is connected on the output shaft of driving motor,Multiple paddles are connected on agitating shaft,It is provided with electric heating wire on the madial wall of culture tank,The front of mounting base is provided with thermoregulation knob and controller.The device has broad application prospects.

Description

Suspension culture device
Technical field
The utility model is related to technical field of biological culture, and specially suspend culture device.
Background technology
In biological medicine production, especially in the production of vaccine, need to cultivate the inoculation that some cells carry out virus, and The culture of cell generally uses culture bottle or rolling bottle.When carrying out cell culture using culture bottle or rolling bottle, cell is all It grows on bottle inner wall, is limited to the area of bottle inner wall, the cell total amount turned out has received the limitation of area, and yield is small, It is not suitable for the requirement produced in enormous quantities, therefore, is badly in need of suspension culture device currently on the market.
Utility model content
The purpose of this utility model is to provide suspension culture devices, to solve the problems mentioned in the above background technology.
To achieve the above object, the utility model provides the following technical solutions:Suspension culture device, including mounting base and The top of the culture tank being fixed in mounting base, the culture tank is equipped with and its seal with screwed joint lid, the culture tank Side be equipped with the woven hose that communicates therewith, and the end of the woven hose is connected with nutrient solution and provides device, the woven hose In be provided with the first solenoid valve and fluid flowmeter, and fluid flowmeter setting provides dress in the first solenoid valve and nutrient solution Between setting, the air inlet pipe being connected to the culture tank is provided with below the woven hose, and the inlet end of the air inlet pipe connects It is connected to air filter, aspiration pump is installed in the air inlet pipe, is set in the air inlet pipe between the aspiration pump and culture tank It is equipped with second solenoid valve and air flow meter, and the air flow meter is arranged between second solenoid valve and aspiration pump, it is described The other side of culture tank is provided with the exhaust pipe communicated therewith, and third solenoid valve is provided in the exhaust pipe, the exhaust The lower section of pipe is equipped with the sewer pipe being connected to the culture tank, and the end of the sewer pipe is connected to a collecting tank, described useless 4th solenoid valve is installed, the mounting base is internally provided with driving motor, and the output shaft of the driving motor in liquid pipe On be connected with the agitating shaft extending into culture tank, multiple paddles, the culture are from top to bottom connected on the agitating shaft Electric heating wire is provided on the madial wall of tank, and the front of the mounting base is provided with thermoregulation knob, the front of the mounting base It is provided with controller, and first solenoid valve, second solenoid valve, third solenoid valve, the 4th solenoid valve, fluid flowmeter, sky Air-flow gauge and driving motor are electrically connected with the controller, the sewer pipe between the 4th solenoid valve and culture tank Inside it is provided with semi-permeable membrane.
Further, it is provided with external screw thread on the lateral wall of the culture upper end, and on the madial wall of the sealing cover It is provided with the internal thread to match with the external screw thread.
Further, the front of the mounting base is provided with the display screen being electrical connected with the controller.
Further, the driving motor is servo motor.
Further, the length of paddle is from top to bottom gradually incremented by the agitating shaft.
Compared with prior art, the utility model has the beneficial effects that:The utility model is provided in the both sides of culture tank Woven hose, air inlet pipe, exhaust pipe and sewer pipe are conducive to the culture of cell in this way;Stirring on agitating shaft in the utility model It mixes leaf length to be from top to bottom gradually incremented by, paddle can be stirred it, be convenient for the suspension culture of cell;Meanwhile this reality Electric heating wire is also installed in culture tank with novel, convenient for the temperature in control culture tank, therefore, the practicability of the culture tank It is extremely strong, it is suitble to promotion and application on the market.
Description of the drawings
Fig. 1 is the overall structure diagram of suspension culture device in the utility model;
Fig. 2 is the schematic cross-section of mounting base in the utility model;
In reference numeral:1- mounting bases;2- culture tanks;3- sealing covers;4- woven hoses;5- nutrient solutions provide device;6- One solenoid valve;7- fluid flowmeters;8- air inlet pipe;9- air filters;10- aspiration pumps;11- second solenoid valves;12- air Flowmeter;13- exhaust pipes;14- third solenoid valves;15- sewer pipes;16- collecting tanks;The 4th solenoid valves of 17-;18- driving motors; 19- agitating shafts;20- paddles;21- electric heating wires;22- thermoregulation knobs;23- controllers;24- display screens;25- semi-permeable membranes.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work The every other embodiment obtained, shall fall within the protection scope of the present invention.
- 2 are please referred to Fig.1, the utility model provides a kind of technical solution:Suspension culture device, including mounting base 1 and solid The top of the fixed culture tank 2 being arranged in mounting base 1, the culture tank 2 is equipped with and its seal with screwed joint lid 3, the culture The side of tank 2 is equipped with the woven hose 4 communicated therewith, and the end of the woven hose 4 is connected with nutrient solution and provides device 5, described The first solenoid valve 6 and fluid flowmeter 7 are provided in woven hose 4, and the fluid flowmeter 7 is arranged in 6 He of the first solenoid valve Between nutrient solution provides device 5, the lower section of the woven hose 4 is provided with the air inlet pipe 8 being connected to the culture tank 2, and described The inlet end of air inlet pipe 8 is connected with air filter 9, and aspiration pump 10,10 He of the aspiration pump are equipped in the air inlet pipe 8 Second solenoid valve 11 and air flow meter 12 are provided in air inlet pipe 8 between culture tank 2, and the air flow meter 12 is arranged Between second solenoid valve 11 and aspiration pump 10, the other side of the culture tank 2 is provided with the exhaust pipe 13 communicated therewith, and institute It states and is provided with third solenoid valve 14 in exhaust pipe 13, the lower section of the exhaust pipe 13 is equipped with the waste liquid being connected to the culture tank 2 Pipe 15, and the end of the sewer pipe 15 is connected to a collecting tank 16, and the 4th solenoid valve 17, institute are equipped in the sewer pipe 15 That states mounting base 1 is internally provided with driving motor 18, and is connected on the output shaft of the driving motor 18 and extend into culture tank 2 Interior agitating shaft 19 is from top to bottom connected with multiple paddles 20 on the agitating shaft 19, is set on the madial wall of the culture tank 2 It is equipped with electric heating wire 21, and the front of the mounting base 1 is provided with thermoregulation knob 22, the front of the mounting base 1 is provided with control Device 23 processed, and first solenoid valve 6, second solenoid valve 11, third solenoid valve 14, the 4th solenoid valve 17, fluid flowmeter 7, Air flow meter 12 and driving motor 18 are electrically connected with the controller 23, the 4th solenoid valve 17 and culture tank 2 Between sewer pipe in be provided with semi-permeable membrane 25.
Controller can be microcontroller or CPU for controlling connected equipment or carrying out data exchange.
Further, external screw thread, and the madial wall of the sealing cover 3 are provided on the lateral wall on 2 top of the culture tank On be provided with the internal thread to match with the external screw thread;Sealing cover 3 is opened using being threadedly coupled.
Further, the front of the mounting base 1 is provided with the display screen 24 being electrical connected with the controller 23;It can use Indices in display culture tank.
Further, the driving motor 18 is servo motor;Convenient for controlling mixing speed.
Further, the length of paddle 20 is from top to bottom gradually incremented by the agitating shaft 19.
Operation principle:In use, inputting culture into culture tank 2 by nutrient solution offer device 5 and woven hose 4 first Liquid opens sealing cover 3, rill is put into culture tank 2, the temperature in culture tank 2 is controlled by thermoregulation knob 22 at this time, leads to It crosses aspiration pump 10 and is sent into clean air into culture tank 2 and breathed for cell, after the completion of culture, opened by controller 23 4th solenoid valve 17 so that, waste liquid can be flowed by semi-permeable membrane 25 in collecting tank 16, and cell can be trapped in culture tank 2, Culture tank 2 can be discharged by exhaust pipe 13 in the exhaust gas that cellular respiration generates, and the practicability of the device is extremely strong, be suitble to push away on the market Extensively.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is appreciated that can these embodiments be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaiied Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.

Claims (6)

1. suspension culture device, which is characterized in that including mounting base(1)And it is fixed at mounting base(1)On culture tank (2), the culture tank(2)Top be equipped with and its seal with screwed joint lid(3), the culture tank(2)Side be equipped with and its The woven hose of connection(4), and the woven hose(4)End be connected with nutrient solution provide device(5), the woven hose(4)In It is provided with the first solenoid valve(6)And fluid flowmeter(7), and the fluid flowmeter(7)It is arranged in the first solenoid valve(6)And battalion Nutrient solution provides device(5)Between, the woven hose(4)Lower section be provided with and the culture tank(2)The air inlet pipe of connection(8), And the air inlet pipe(8)Inlet end be connected with air filter(9), the air inlet pipe(8)On aspiration pump is installed(10), The aspiration pump(10)And culture tank(2)Between air inlet pipe(8)Inside it is provided with second solenoid valve(11)And air flow meter (12), and the air flow meter(12)It is arranged in second solenoid valve(11)And aspiration pump(10)Between, the culture tank(2)'s The other side is provided with the exhaust pipe communicated therewith(13), and the exhaust pipe(13)Inside it is provided with third solenoid valve(14), described Exhaust pipe(13)Lower section be equipped with and the culture tank(2)The sewer pipe of connection(15), and the sewer pipe(15)End with One collecting tank(16)Connection, the sewer pipe(15)4th solenoid valve is inside installed(17), the mounting base(1)Inside setting There is driving motor(18), and the driving motor(18)Output shaft on be connected with and extend into culture tank(2)Interior agitating shaft (19), the agitating shaft(19)On be from top to bottom connected with multiple paddles(20), the culture tank(2)Madial wall on be arranged There is electric heating wire(21), and the mounting base(1)Front be provided with thermoregulation knob(22).
2. suspension culture device according to claim 1, which is characterized in that the mounting base(1)Front be provided with control Device processed(23), and first solenoid valve(6), second solenoid valve(11), third solenoid valve(14), the 4th solenoid valve(17), liquid Flowmeter body(7), air flow meter(12)And driving motor(18)The equal and controller(23)It is electrically connected, the described 4th Solenoid valve(17)And culture tank(2)Between sewer pipe in be provided with semi-permeable membrane(25).
3. suspension culture device according to claim 1, which is characterized in that the culture tank(2)On the lateral wall on top It is provided with external screw thread, and the sealing cover(3)Madial wall on be provided with the internal thread to match with the external screw thread.
4. suspension culture device according to claim 2, which is characterized in that the mounting base(1)Front be provided with The controller(23)The display screen being electrical connected(24).
5. suspension culture device according to claim 1, which is characterized in that the driving motor(18)For servo motor.
6. suspension culture device according to claim 1, which is characterized in that the agitating shaft(19)Upper paddle(20)'s Length is from top to bottom gradually incremented by.
CN201721722873.9U 2017-12-12 2017-12-12 Suspension culture device Active CN207685305U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201721722873.9U CN207685305U (en) 2017-12-12 2017-12-12 Suspension culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201721722873.9U CN207685305U (en) 2017-12-12 2017-12-12 Suspension culture device

Publications (1)

Publication Number Publication Date
CN207685305U true CN207685305U (en) 2018-08-03

Family

ID=62997398

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201721722873.9U Active CN207685305U (en) 2017-12-12 2017-12-12 Suspension culture device

Country Status (1)

Country Link
CN (1) CN207685305U (en)

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