CN207646204U - Digital pcr droplet generating means - Google Patents

Abstract

The utility model embodiment provides a kind of digital pcr droplet generating means, including gas pressurized component, one end connects the pipeline of gas pressurized component, the valve being arranged on pipeline, the cylinder manifold being connect with the pipeline other end and the chip being connect with cylinder manifold, chip is equipped with oil phase pond, water phase pond and droplet collecting pit, cylinder manifold is equipped with outlet and is respectively corresponding to oil phase pond and water phase pond, draw the first microchannel in oil phase pond, the second microchannel is drawn in water phase pond, and the first microchannel and the second microchannel are converged to form third microchannel unicom to droplet collecting pit.Pass through above-mentioned setting, gas pressure is set to act on chip, the fluid in oil phase pond is driven to be flowed along the first microchannel, model liquid in water phase pond is flowed along the second microchannel, two kinds of liquid, which cross, to be generated fluid package sample droplets and flow to droplet collecting pit along third microchannel, realize droplet generation, and the droplet volume uniformity generated.

Description

Digital pcr droplet generating means

Technical field

The utility model belongs to Microfluidic droplet digital polymerase chain reaction (PCR) technical field, more particularly to a kind of Digital pcr droplet generating means.

Background technology

Digital pcr (Polymerase Chain Reaction PCRs) is that a kind of nucleic acid molecules are absolutely fixed Amount technology, digital pcr system are the system detected using digital pcr technology, when carrying out drop detection, by power source by sample It is mixed by Pipeline transport to digital pcr detection chip with dilution.

Currently, digital pcr can be divided into two kinds according to the generating mode of droplet, microwell plate chip digital PCR and drop are digital PCR.Microwell plate chip digital PCR uses high density microwell array chip, and PCR reaction systems are distributed into micropore, have been expanded Cheng Hou, the one by one fluorescence signal of each reacting hole of image checking；Drop digital pcr uses the Water-In-Oil drop based on micro-fluidic chip Every part of PCR reaction system is divided into picoliters tens thousand of to millions of, of uniform size to nanoliter level droplet, each droplet by technology It is all used as an independent PCR reaction member, after the completion of amplification, detects the fluorescence signal of each droplet one by one.In clinical practice During use, it has been found that current microwell plate chip digital PCR product have the following problems in one or more：First Be analyze sample flux it is relatively low, be not suitable for the requirement of clinical high throughput analysis；Secondly, microwell plate chip digital PCR system Expensive, related chip is more complicated, and chip cost is higher, higher operating costs, is unfavorable for its promotion and popularization；Third, behaviour Fail to accomplish as flow totally-enclosed, does not meet the requirement of clinical analysis of diagnosis.

The droplet that theoretically digital pcr micro-fluidic chip provides is more with respect to the number of droplets that micropore board chip provides, and leads to Higher is measured, cost is lower, and sensitivity and acquired results are more acurrate, but the volume of droplet that current droplet generating means are generated Uniformity consistency need to be improved.

Utility model content

The purpose of this utility model is to provide a kind of digital pcr droplet generating means, realizes that droplet generates, and generate Droplet volume uniformity.

To realize that the purpose of this utility model, the utility model provide the following technical solution：

In a first aspect, the utility model provides a kind of digital pcr droplet generating means, including gas pressurized component, one End connects the pipeline of the gas pressurized component, the valve on the pipeline is arranged, the remittance being connect with the pipeline other end Flowing plate and the chip being connect with the cylinder manifold, the chip are equipped with oil phase pond, water phase pond and droplet collecting pit, the confluence Plate is equipped with outlet and is respectively corresponding to the oil phase pond and the water phase pond, and the first microchannel, the water phase are drawn in the oil phase pond The second microchannel is drawn in pond, and first microchannel and second microchannel are converged to form third microchannel unicom to described micro- Drip collecting pit.

Wherein, the pipeline includes first pipe, and the valve includes pressure reducing valve, the first proportioning valve, the second proportioning valve, One solenoid valve and second solenoid valve, the cylinder manifold include first entrance and second entrance, and the pressure reducing valve is set to described On one pipeline, the first pipe is divided into second pipe and third pipeline after the pressure reducing valve, on the second pipe successively Equipped with first proportioning valve and first solenoid valve, second proportioning valve and described is equipped on the third pipeline successively Second solenoid valve, the second pipe are connected to the first entrance, and the third pipeline is connected to second entrance.

Wherein, cylinder manifold outlet includes first outlet and second outlet, the first outlet and the oil phase pond pair Answer, the second outlet is corresponding with the water phase pond, the first entrance by the first passage unicom in the cylinder manifold extremely The first outlet, the second entrance pass through the second channel unicom in the cylinder manifold to the second outlet.

Wherein, the first passage is equipped with first pressure sensor, and the second channel is equipped with second pressure sensor, institute State size of the first pressure sensor for detecting and feeding back the air pressure for being applied to the oil phase pond, the second pressure sensor Size for detecting and feeding back the air pressure for being applied to the water phase pond.

Wherein, the first outlet and the second outlet are equipped with sealing ring, are set between the cylinder manifold and the chip There are seal gasket, the sealing ring to be carried out jointly to the cylinder manifold and the chip link position with the seal gasket close Envelope.

Wherein, the cylinder manifold described in 8 groups equipped with exporting, and the chip is equipped with oil phase pond and the water phase described in 8 groups Pond.

Wherein, further include shell, the shell is equipped with opening, and moveable base station, the core are equipped in the shell Piece is set on the base station, and the base station is connect with the first motion module, and first motion module drives the base station to stretch Go out the opening and retract in the shell, the cylinder manifold is connect with the second motion module, the second motion module driving The relatively described chip of the cylinder manifold moves up and down.

Wherein, first motion module includes first motor, the first executing agency being connected with the first motor；Institute It includes the second motor to state the second motion module, and the second executing agency being connected with second motor is additionally provided in the shell Rack, the rack are fixed on shell, and sliding rail is additionally provided in the rack, and the base station is set on the sliding rail, institute It states first motor to be installed in the rack, first executing agency is fixed on the sliding rail, the second motor installation In in the rack, second executing agency is connected on second motor output shaft and is connected to the cylinder manifold.

Wherein, second motor is linear stepping motor, and second executing agency includes and second motor The rod piece of axis connection is exported, the output shaft of second motor is extended and the rod piece is driven to compress the cylinder manifold.

Wherein, the rod piece compresses one end with the cylinder manifold and is also associated with a S type pull pressure sensor, and the S types are drawn Pressure sensor is for detecting the pressure value size that the rod piece applies the cylinder manifold.

The beneficial effects of the utility model：

Digital pcr droplet generating means provided by the utility model pass through setting gas pressurized component and the pipeline of connection And valve, air pressure is transmitted to cylinder manifold, cylinder manifold outlet corresponds to oil phase pond and water phase pond, gas pressure is made to act on core respectively Piece drives the fluid in oil phase pond to be flowed along the first microchannel, and the sample liquid in water phase pond is flowed along the second microchannel, two kinds of liquid Body, which crosses, to be generated fluid package sample droplets and flow to droplet collecting pit along third microchannel, realizes droplet generation, and raw At droplet volume uniformity.

Description of the drawings

It, below will be to implementing in order to illustrate more clearly of the utility model embodiment or technical solution in the prior art Mode or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, the accompanying drawings in the following description Only some embodiments of the utility model are not making the creative labor for those of ordinary skill in the art Under the premise of, other drawings may also be obtained based on these drawings.

Fig. 1 is the principle schematic of the digital pcr droplet generating means of the utility model embodiment.

Fig. 2 is the structural schematic diagram of the chip in Fig. 1.

Fig. 3 a are the dimensional structure diagrams of the cylinder manifold in Fig. 1.

Fig. 3 b are another view stereo structural schematic diagrams of the cylinder manifold in Fig. 1.

Fig. 3 c are another view stereo structural schematic diagrams of the cylinder manifold in Fig. 1.

Fig. 4 a are the structural schematic diagrams for the digital pcr droplet generating means that the utility model embodiment provides, wherein omitting Pipeline and electric connection line in the shell side plate and shell of side.

Fig. 4 b are the surface structure schematic diagrams of the digital pcr droplet generating means in Fig. 4 a.

Fig. 5 a are the partial structural diagrams of the digital pcr droplet generating means in Fig. 4 a.

Fig. 5 b are the partial structurtes exploded perspective views of the digital pcr droplet generating means in Fig. 4 a.

Fig. 5 c are another visual angle exploded perspective views of partial structurtes of the digital pcr droplet generating means in Fig. 5 a.

Fig. 6 a are another partial structural diagrams of the digital pcr droplet generating means in Fig. 4 a, and shell is omitted in figure And the structures such as pipeline, valve.

Fig. 6 b are another visual angle schematic diagrames of another partial structurtes of the digital pcr droplet generating means in Fig. 6 a.

Specific implementation mode

Below in conjunction with the attached drawing in the utility model embodiment, to the technical solution in the utility model embodiment It being clearly and completely described, it is clear that described embodiment is only a part of embodiment of the utility model, without It is whole embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not making creation Property labour under the premise of the every other embodiment that is obtained, shall fall within the protection scope of the present invention.

It please refers to Fig.1 to Fig. 2, a kind of better embodiment of the utility model provides a kind of digital pcr droplet generation dress It sets, including gas pressurized component 1, one end connects the pipeline of the gas pressurized component 1, and the valve on the pipeline is arranged, The cylinder manifold 200 being connect with the pipeline other end and the chip 100 being connect with the cylinder manifold 200 are set on the chip 100 There are oil phase pond 110, water phase pond 120 and droplet collecting pit 130, the cylinder manifold 200 to be equipped with outlet and be respectively corresponding to the oil phase The first microchannel 111 is drawn in pond 110 and the water phase pond 120, the oil phase pond 110, and it is micro- logical that the water phase pond 120 draws second Road 121, first microchannel 111 and second microchannel 121 are converged to form 141 unicom of third microchannel to the droplet Collecting pit 130.

In present embodiment, by the way that the pipeline and valve of gas pressurized component 1 and connection is arranged, air pressure is transmitted to remittance Flowing plate 200, the outlet of cylinder manifold 200 correspond to oil phase pond 110 and water phase pond 120, gas pressure are made to act on chip 100, drive respectively The fluid in oil phase pond 110 is set to be flowed along the first microchannel 111, the sample liquid in water phase pond 120 is flowed along the second microchannel 121 Dynamic, two kinds of liquid, which cross, to be generated fluid package sample droplets and flow to droplet collecting pit 130 along third microchannel 141, is realized Droplet generates, and the droplet volume uniformity generated.

Wherein, gas pressurized component 1 is used to generate clean steady with dry compressed gas, such as the properties such as nitrogen or air Fixed gas.

Pipeline is used to convey the compressed gas of the generation of gas pressurized component 1 to cylinder manifold 200, and valve is for adjusting or being opened and closed Compressed gas so that the compressed gas air pressure for entering cylinder manifold 200 changes.

Chip 100 can be used injection molding duroplasts and be made, and 100 integral hardness value of chip is consistent so that chip by Each microchannel is indeformable when pressure.The concrete shape and structure of chip 100 are not limited to upper layer and lower layer or upper, middle and lower three-decker.

For storing fluid, water phase pond 120 is used for stored samples liquid in oil phase pond 110, and chip 100 is into present embodiment Droplet generating means before enclose fluid and sample liquid in oil phase pond 110 and water phase pond 120, operating process can be with For：Chip 100 is placed in the plane, using pipettor sampling, oiling liquid is loaded this liquid and arrives water phase pond to oil phase pond 110 respectively 120, pipettor can be cuvette construction.Oil phase pond 110 and water phase pond 120 can protrude from the setting of 100 surface of chip, can also To open up the form of groove on chip 100.The volume in oil phase pond 110 is not less than the volume in water phase pond 120, since fluid wraps up The demand of model droplet, fluid answers bigger compared to the demand of sample liquid, and therefore, the volume in oil phase pond 110 may be slightly larger than water The volume in phase pond 120, so that the fluid stored in oil phase pond 110 is more compared to the amount of the model liquid stored in water phase pond 120.

Fluorination liquid can be used in fluid, such as the HFE7500 that 3M companies provide, and the segmentation sample liquid that act as of fluid is sample Droplet makes each sample droplets be isolated, in order to subsequent pattern detection.

First microchannel 111 may include 2, extend along 2 parallel channels after being drawn from oil phase pond 110, extension Direction can be the direction towards water phase pond 120, and 2 parallel channels start close and converge to droplet after extending a distance into It can be the position close to water phase pond 120 that the parallel channel in formation zone 140,2, which starts close position,.

Second microchannel 121 is 1, logical along 2 of linear extension to the first microchannel 111 after being drawn from water phase pond 120 Road confluence area, i.e. droplet formation zone 140, fluid and sample liquid form fluid package after droplet formation zone is converged The droplet structure of sample droplets.

Third microchannel 141 is 1, along linear extension to droplet collecting pit 130 after being drawn from droplet formation zone 140, The liquid that fluid wraps up sample droplets is stored in droplet collecting pit 130.

Wherein, oil phase pond 110, water phase pond 120 and droplet collecting pit 130 are arranged along straight line so that the first microchannel 111, the path of the second microchannel 121 and third microchannel 141 can reduce flow resistance, make effect substantially along linear extension It can be reduced in the gas pressure in oil phase pond 110 and water phase pond 120, reduce energy consumption.First microchannel 111, the second microchannel 121 It is not limited herein with the specific size of third microchannel 141, fluid and sample liquid energy need to only be made to form droplet structure and smooth By, it is however generally that, each microchannel should form the structure of similar capillary from macroscopically seeing.

Further, the cylinder manifold 200 described in 8 groups equipped with exporting, and the chip is equipped with oil phase pond 110 described in 8 groups With the water phase pond 120.By so set, 8 droplet structures can once be formed, in addition, in 8 groups of oil phase ponds 110 and water phase The fluid and sample liquid stored in pond 120 can be different, to obtain different droplet structures.

It in a kind of embodiment, please refers to Fig.1, Fig. 3 a, Fig. 3 b and Fig. 3 c, the pipeline includes first pipe 11, described Valve includes pressure reducing valve 2, the first proportioning valve 4, the second proportioning valve 5, the first solenoid valve 6 and second solenoid valve 7, the cylinder manifold 200 include first entrance 201 and second entrance 203, and the pressure reducing valve 2 is set in the first pipe 11, first pipe Road 11 is divided into second pipe 12 and third pipeline 13 after the pressure reducing valve 2, is equipped with described the on the second pipe 12 successively One proportioning valve 4 and first solenoid valve 6 are equipped with second proportioning valve 5 and described second on the third pipeline 13 successively Solenoid valve 7, the second pipe 12 are connected to the first entrance 201, and the third pipeline 13 is connected to second entrance 203.

Wherein, size of the pressure reducing valve 2 for regulating and controlling 11 pressure of first pipe, the first proportioning valve 4 is for regulating and controlling second pipe The size of 12 pressure, size of second proportioning valve 5 for regulating and controlling 13 pressure of third pipeline, the first solenoid valve 6 is for controlling second The break-make of pipeline 12, second solenoid valve 7 are used to control the break-make of third pipeline 13.

By above-mentioned setting, the high pressure gas that gas pressurized component 1 exports is when through first pipe 11, due to pressure reducing valve 2 Effect, gas pressure reduce, pressure reducing valve 2 to compressed gas be depressurized to gas pressure be slightly larger than oil phase pond 110 and water phase pond 120 Required pressure；Again by the first proportioning valve 4 to second pipe 12 regulate and control pressure value size and the second proportioning valve 5 to third pipe Road 13 regulates and controls the size of pressure value so that the pressure value in second pipe 12 and third pipeline 13 can be different, so that gas is made For the pressure value in oil phase pond 110 and water phase pond 120 in respective setting range, ensure first pipe 12 and second pipe 13 Gas pressure driving fluid and sample liquid keep relative equilibrium flow velocity, make the droplet uniformity of generation.

Wherein, the first solenoid valve 6 and second solenoid valve 7 can be turned on and off second pipe 12 and third pipeline 13, so as to New droplet is carried out in replacement chip 100 to generate.

Wherein, pressure gauge 3 can be set after pressure reducing valve 2 in first pipe 11, the after being depressurized for detection pressure reducing valve 2 Air pressure size in one pipeline 11；On second pipe 12 after the first proportioning valve 4 and on third pipeline 13 after the second proportioning valve 5 Pressure gauge (not shown) is may also set up, to detect the air pressure size in second pipe 12 and third pipeline 13 respectively.

Further, it please refers to Fig.3 and Fig. 4, the outlet of the cylinder manifold 200 includes first outlet 230 and second outlet 240, the first outlet 230 is corresponding with the oil phase pond 110, and the second outlet 240 is corresponding with the water phase pond 120, institute First entrance 201 is stated by the first passage (not shown) unicom in the cylinder manifold 200 to the first outlet 230, The second entrance 203 passes through the second channel (not shown) unicom in the cylinder manifold 200 to the second outlet 240。

Wherein, cylinder manifold 200 is the platy structure of global formation, and cylinder manifold 200 can be in cubical shape comprising The upper and lower surface being oppositely arranged, and the leading flank being oppositely arranged and trailing flank and the left side that is oppositely arranged and Right side, wherein first entrance 201 and second entrance 203 are respectively arranged on leading flank and trailing flank, and first outlet 230 and second go out Mouth 240 is set to lower surface, and first passage and second channel are set to inside cylinder manifold 200, and first passage and second channel are only Vertical channel is not connected to and intersects each other；The structure of first passage and second channel can be the pipeline dug out in cylinder manifold, Or by piping laying in the form in cylinder manifold.200 upper surface of cylinder manifold is additionally provided with the first mounting hole 210 and second peace Hole 220 is filled, the first mounting hole 210 and the second mounting hole 220 can be Screw hole structure, and not be connected to first passage and second Channel, to install cylinder manifold 200.

Further, a to Fig. 3 c, Fig. 4 a, Fig. 5 a to Fig. 5 c are please referred to Fig.3, the first passage is passed equipped with first pressure Sensor 91, the second channel are equipped with second pressure sensor 92, and the first pressure sensor 91 is used to detect and feedback is applied The size of the air pressure in the oil phase pond 110 is added to, the second pressure sensor 92 is used to detect and feedback is applied to the water The size of the air pressure in phase pond 120.

Wherein, the first entrance 201 of cylinder manifold 200 is through front and back sides and by the first plate hole 204 is with extraneous unicom First passage is formed, i.e. first passage is that first entrance 201 is penetrated through to the channel of the first plate hole 204, before second entrance 203 runs through Trailing flank simultaneously forms second channel from opposite the second plate hole of leading flank 202 and extraneous unicom, i.e., second channel enters for second 203 perforation of mouth to the channel of the second plate hole 202, first pressure sensor 91 stretches into first passage, the second pressure from the first plate hole 204 Force snesor 92 stretches into second channel by the second plate hole 202.First plate hole 204 and the second plate hole 202 can be screw hole, first Pressure sensor 91 and second pressure sensor 92 can have helicitic texture, first pressure sensor 91 and the first plate hole 204 It is threadedly coupled and closes first passage, second pressure sensor 92 is threadedly coupled with the second plate hole 202 and closes second channel.

Wherein, first entrance 201 is connect with the first pipe fitting 81, and second entrance 203 is connect with the second pipe fitting 82, and first Pipe fitting 81 and the second pipe fitting 82 are connect with second pipe 12 and third pipeline 13 respectively.

Further, a to Fig. 3 c, Fig. 5 a to Fig. 5 c, the first outlet 230 and the second outlet 240 are please referred to Fig.3 Equipped with sealing ring 300, seal gasket 400, the sealing ring 300 and institute are equipped between the cylinder manifold 200 and the chip 100 Seal gasket 400 is stated jointly to be sealed the cylinder manifold 200 and 100 link position of the chip.

Wherein, the first boss 231 for protruding from 200 surface of cylinder manifold, first boss 231 can be arranged in first outlet 230 It is arranged around first outlet 230, sealing ring 300 can be placed in first boss 231, second outlet 240 and first outlet 230 It is similar, second boss 241 can also be set and sealing ring 300 is sheathed in second boss 241.Oil phase pond on chip 100 110 and water phase pond 120 be correspondingly arranged on the seal gasket 400 enclosed around oil phase pond 110 and water phase pond 120 1, cylinder manifold 200 is pressed Firmly when chip 100, sealing ring 300 is fitted closely with seal gasket 400 so that the first outlet 230 of cylinder manifold 200 and second goes out The compressed gas being passed through in mouth 240 will not be revealed, and pressurize to oil phase pond 110 and water phase pond 120.In other embodiments, First outlet 110 and second outlet 120 or 200 surface of cylinder manifold dig the form of groove (not shown) for Accommodating sealing ring 300.

Further, sealing ring 300 may include 2 and correspond to first boss 231 respectively and second boss 241,2 is close Seal 300 can be with different structures to be respectively received in first boss 231 and the circular space of second boss 241.

Further, on seal gasket 400 have on chip 100 oil phase pond 110 and 120 position of water phase pond it is corresponding Opening so that the first outlet 230 and second outlet 240 of cylinder manifold 200 pass through corresponding opening unicom on seal gasket to oil Phase pond 110 and water phase pond 120.

The material of sealing ring 300 and seal gasket 400 is, for example, rubber, preferred sealing ring 300 and seal gasket 400 Material is identical, so that the two can combine closely when compressing.

In a kind of embodiment, a and Fig. 4 b are please referred to Fig.4, pipeline and electric connection line is omitted in wherein Fig. 4 a, further includes shell Body 20, the shell 20 are equipped with opening 21, are equipped with moveable base station 40 in the shell 20, the chip 100 is set to On the base station 40, the base station 40 is connect with the first motion module (non-label in figure), and first motion module drives institute It states base station 40 to stretch out the opening 21 and retract in the shell 20, the cylinder manifold 200 (is not marked with the second motion module in figure Number) connection, the second motion module driving 200 relatively described up and down motion of chip 100 of cylinder manifold.

Please also refer to Fig. 4 a, Fig. 4 b, Fig. 6 a and Fig. 6 b, shell 20 have it is opposite up and down, left and right and front and rear panel, have Dismountable structure is also provided with assistant reinforcement plate (such as the lower plate opposite with shell connected with shell 20 in shell 20 The reinforcing plate 250 of connection), assistant reinforcement plate can install the tablet of computer main board in similar computer housing, each in the present apparatus A parts are mountable on assistant reinforcement plate, can bumper housing 20 and each parts impact, also allow for arranging each zero The space layout of component so that there is certain interval, convenient for air flows and radiates between each parts and shell 20.It should Understand, being installed on shell 20 also described in the utility model includes situation about being installed on assistant reinforcement plate.Opening 21 can A normally closed door-plate (not shown) is arranged can keep the envelope with shell 20 for closing shell 20, door-plate by spring structure It closes, when base station 40 moves, the edge of base station 40 drives door-plate to be opened against the door-plate, when in 40 retraction shell 20 of base station When, door-plate rebound under the action of the spring continues to keep closing, can be used for realizing that the overall integrity of shell, dust-proof protection against insect are old Mouse etc..

Further, first motion module includes first motor 38, and first to be connected with the first motor 38 is held Row mechanism (non-label in figure)；Second motion module includes the second motor 37, second to be connected with second motor 37 Executing agency's (non-label in figure) is additionally provided with rack 41 in the shell 20, and the rack is fixed on shell 20, the rack Sliding rail 391 is additionally provided on 41, the base station 40 is set on the sliding rail 391, and the first motor 38 is installed on the machine On frame 41, first executing agency is fixed on the sliding rail 391, and second motor 37 is installed in the rack 41, Second executing agency is connected on 37 output shaft of the second motor and is connected to the cylinder manifold 200.

Wherein, the shape of rack 41 is c-type, i.e. rack 41 includes opposite top and bottom, and top and bottom pass through branch Brace connects, and lower end is mounted on the bottom plate of shell 20, and can also be mounted on can be in the reinforcing plate 250 of bottom plate, base station 40 be set It is placed in the lower end of rack 41, the second motor 37 is installed on the upper end of rack 41, and first motor 38 is installed on the support arm of rack 41 On.In present embodiment, first executing agency is gear ＆ rack structure, and the rack 39 is installed on the sliding rail 391, Sliding rail 391 is installed on the shell 20.Wherein, the base station 41 is mounted on rack 39.Sliding rail 391 can also be mounted on machine On frame 41.First motor 38 can be stepper motor, and the gear 381 that the first executing agency coordinates with rack 39 at this time can be installed In on the output shaft of first motor 38；When first motor 38 is common electric machine, it can set and subtract on the output shaft of first motor 38 Fast structure is connect with gear 381.It is slided on sliding rail 391 by 38 drive rack 39 of first motor, drives and connect with rack 39 The base station 40 connect moves linearly.Wherein, sliding rail 391 is linear structure, and is extended towards the direction of opening 21 in shell 20. In other embodiments, first executing agency can also be the forms such as toothed belt transmission, lead screw transmission or rope drive, The sliding rail 391 can be V-type sliding rail, dovetail groove sliding rail or optical axis guide rail etc..

Fig. 5 a to Fig. 5 c are please referred to, the connecting hole 42 being connect with rack 39 is provided on base station 41.The droplet of chip 100 is received The form that droplet outlet 131 can be arranged in collection pond 130 by opposite another side stores the droplet being collected into special container, Such as in eight unions 401 of PCR.

Further, second motor 37 is linear stepping motor, and second executing agency includes and described second The output shaft of the rod piece 371 of the output axis connection of motor 37, second motor 37 is extended and the rod piece 371 is driven to compress The cylinder manifold 200, rod piece 371 compresses one end with cylinder manifold 200 can also be connected with a S types pull pressure sensor 372, and S types are drawn The pressure value size that the effect detection rod piece 371 of pressure sensor 372 applies cylinder manifold 200.In another embodiment, Pressing plate 411 moving up and down is additionally provided on the support arm of rack 41, the output shaft stretching of the second motor 37 resists pressing plate 411, Pressing plate 411 moves down and compresses cylinder manifold 200, to make the moving up and down on defined track of pressing plate 411, in rack 41 It is fixed with a slide plate 412 on support arm, there is the opposite trench structure (not shown) extended up and down, pressure on slide plate 412 Plate 411 has the structure that coordinate with the trench structure, and such setting can make the moving up and down always in slide plate 412 of pressing plate 411 Under trench structure limitation, ensures that pressing plate 412 is moved up and down along trench structure without deflection always, ensure pressing plate 412 to converging The pressure of flowing plate 200 is uniform.Cylinder manifold 200 can be connect with rod piece 371 or pressing plate 411 in advance, then 37 output shaft of the second motor When extending downwardly, cylinder manifold 200, which follows, to be moved down and contacts and compress with chip 100, it is furthermore preferred that rod piece is arranged simultaneously 371 and pressing plate 411, cylinder manifold 200 connect in advance with pressing plate 411, rod piece 371 drive pressing plate 411 slide plate 412 trench structure It inside moves up and down, the measure of precision for being greatly improved equipment is arranged such, ensure that pressing plate 411 compresses cylinder manifold 200.It should manage Solution, whether which kind of embodiment, left and right are that cylinder manifold 200 is made to be fitted closely with chip 100, are made on cylinder manifold 200 Outlet is corresponding with the oil phase pond 110 of chip 100 and water phase pond 120, and gas pressure may act on oil phase pond 110 and water phase pond 120.

Further, power supply 31, wind turbine 32, master control borad 35 and motor driving plate 36 are additionally provided in the shell 20, it is described Display screen 22, gas pressurized mouth 34 are additionally provided on shell 20, the motor driving plate 36 is for controlling 38 He of the first motor Second motor 37, the gas pressurized component 1 are connect by first pipe 11 with the gas pressurized mouth 34.

Wherein, it can be DC power supply to have power switch 33, power supply 31 on shell 20, and the setting of wind turbine 32 is installed on shell 20 On, the air in shell 20 can be accelerated to flow, for carrying out radiating and cooling in shell 20.

Master control borad 35 is for information processing and sends out instruction, for example, first pressure sensor 91 and second pressure sensor The pressure information of 92 feedbacks is transferred to master control borad 35, and master control borad 35 is according to preset program pair the first proportioning valve 4, the second proportioning valve 5, the first solenoid valve 6 or second solenoid valve 7 send out instruction, to change the pressure inputted in cylinder manifold 200, it should be pointed out that The decompression action of pressure reducing valve 2 can be also adjusted by being adjusted manually by the instruction of master control borad 35.Motor driving plate 36 send out instruction, it is desirable that are controlled first motor 38 or the second motor 37 opposite with chip 100 to change cylinder manifold 200 Position, or require base station 40 stretching out shell 20 or retraction shell 20 etc..That is, master control borad 35 mainly controls air pressure System, motor driving plate 36 mainly control position, and certainly, in another embodiment, master control borad 35 drives with motor Plate 36 can be merged into one piece of control panel.

Display screen 22 can be touch screen, and first pipe 11, second pipe 12 and third pipeline can be shown on display screen 22 The pressure of gas in 13 may also display first pressure sensor 91, second pressure sensor 92 and S types pull pressure sensor 372 Pressure numerical values recited, may also display the operating parameter etc. of first motor 38 and the second motor 37, on touch screen can also to Upper pressure or parameter etc. are adjusted.

The application method of the present apparatus is described below (please also refer to Fig. 1 to Fig. 6 b)

Each pipeline is connected first, i.e., the first pipe 11 drawn in gas pressurized component 1 is connected to gas pressurized mouth 34, it has been connected in advance before each pipeline in shell 20, i.e. the first pipe of gas pressurized mouth 34 is connected to pressure reducing valve 2, subtracts Pressure valve 2 separates that second pipe 12 is connected to the first proportioning valve 4, third pipeline 13 is connected to the second proportioning valve 5, second pipe 12 from The extraction of first proportioning valve 4 is connected to the first solenoid valve 6, and third pipeline 13 is connected to second solenoid valve from the extraction of the second proportioning valve 5 7, second pipe 12 draws the first entrance 201 being connected on cylinder manifold 200, third pipeline 13 from second from the first solenoid valve 6 Solenoid valve draws the second entrance 203 being connected on cylinder manifold 200；

33 are turned on the power switch, control first motor 38 drives base station 40 to stretch out the outlet 21 on shell 20, will match in advance The chip 100 of good fluid and sample liquid is lain against on base station 40, and first motor 38 is driven again in 40 retraction shell 20 of base station, and Precalculated position is stopped at, which is the oil phase in first outlet 230 and second outlet 240 and chip 100 on cylinder manifold 200 120 corresponding position of pond 110 and water phase pond, the second motor 37 of driving make cylinder manifold 200 be fitted closely with chip 100；

The gas of gas pressurized component 1 enters, and adjusts the pressure value of pressure reducing valve 2, the first proportioning valve 4 and the second proportioning valve 5 To preset pressure value；

Gas pressure on chip 100 oil phase pond 110 and water phase pond 120, the fluid in oil phase pond 110 it is micro- by first Channel 111 is flowed, and the sample liquid in water phase pond 120 is flowed by the second microchannel 121, and the two is converged in droplet formation zone 140 It closes and forms oily packet sample droplets structure and flow into droplet collecting pit 130 through third microchannel 141, complete the generation of droplet；

Droplet in subsequent droplets collecting pit 130 can be connect by outlet eight unions 401 of PCR of droplet collecting pit 130 It stores up and deposits, chip 100 completes after droplet generates and needs to handle, general not reproducible use.

Above disclosed is only a kind of the utility model better embodiment, cannot limit this reality with this certainly With novel interest field, one of ordinary skill in the art will appreciate that realize all or part of flow of the above embodiment, And equivalent variations made according to the claim of the utility model, it still falls within the scope of the utility model.

Claims (10)

1. a kind of digital pcr droplet generating means, which is characterized in that including gas pressurized component, one end connects the gas and adds The pipeline for pressing component, is arranged the valve on the pipeline, the cylinder manifold that is connect with the pipeline other end and with the confluence The chip of plate connection, the chip are equipped with oil phase pond, water phase pond and droplet collecting pit, and it is right respectively that the cylinder manifold is equipped with outlet The first microchannel should be drawn to the oil phase pond and the water phase pond, the oil phase pond, the second microchannel is drawn in the water phase pond, First microchannel and second microchannel are converged to form third microchannel unicom to the droplet collecting pit.

2. digital pcr droplet generating means as described in claim 1, which is characterized in that the pipeline includes first pipe, institute It includes pressure reducing valve, the first proportioning valve, the second proportioning valve, the first solenoid valve and second solenoid valve to state valve, and the cylinder manifold includes First entrance and second entrance, the pressure reducing valve are set in the first pipe, and the first pipe is after the pressure reducing valve It is divided into second pipe and third pipeline, first proportioning valve and first solenoid valve is equipped with successively on the second pipe, Second proportioning valve and the second solenoid valve are equipped on the third pipeline successively, the second pipe is connected to described the One entrance, the third pipeline are connected to second entrance.

3. digital pcr droplet generating means as claimed in claim 2, which is characterized in that the cylinder manifold outlet includes first Outlet and second outlet, the first outlet is corresponding with the oil phase pond, and the second outlet is corresponding with the water phase pond, described First entrance passes through the confluence by the first passage unicom in the cylinder manifold to the first outlet, the second entrance Second channel unicom in plate is to the second outlet.

4. digital pcr droplet generating means as claimed in claim 3, which is characterized in that the first passage is equipped with the first pressure Force snesor, the second channel are equipped with second pressure sensor, and the first pressure sensor is for detecting and feeding back application To the size of the air pressure in the oil phase pond, the second pressure sensor is for detecting and feeding back the gas for being applied to the water phase pond The size of pressure.

5. digital pcr droplet generating means as claimed in claim 3, which is characterized in that the first outlet and described second Outlet is equipped with sealing ring, and seal gasket, the sealing ring and the seal gasket are equipped between the cylinder manifold and the chip The cylinder manifold and the chip link position are sealed jointly.

6. digital pcr droplet generating means as described in claim 1, which is characterized in that the cylinder manifold be equipped with 8 groups described in go out Mouthful, the chip is equipped with oil phase pond and the water phase pond described in 8 groups.

7. digital pcr droplet generating means as described in claim 1, which is characterized in that further include shell, set on the shell There is opening, moveable base station is equipped in the shell, the chip is set on the base station, the base station and the first movement Module connects, and first motion module drives the base station to stretch out the opening and retracts in the shell, the cylinder manifold It is connect with the second motion module, second motion module drives the relatively described chip of the cylinder manifold to move up and down.

8. digital pcr droplet generating means as claimed in claim 7, which is characterized in that first motion module includes the One motor, the first executing agency being connected with the first motor；Second motion module includes the second motor, with described the The second connected executing agency of two motors is additionally provided with rack in the shell, and the rack is fixed on shell, in the rack It is additionally provided with sliding rail, the base station is set on the sliding rail, and the first motor is installed in the rack, and described first holds Row mechanism is fixed on the sliding rail, and second motor is installed in the rack, and second executing agency is connected to institute It states on the second motor output shaft and is connected to the cylinder manifold.

9. digital pcr droplet generating means as claimed in claim 8, which is characterized in that second motor is straight line stepping Motor, second executing agency include the rod piece with the output axis connection of second motor, the output of second motor Axis is extended and the rod piece is driven to compress the cylinder manifold.

10. digital pcr droplet generating means as claimed in claim 9, which is characterized in that the rod piece and the cylinder manifold pressure Tight one end is also associated with a S type pull pressure sensor, and the S types pull pressure sensor is for detecting the rod piece to the confluence The pressure value size that plate applies.