CN102759481B - Multi-cell mechanical simulation experiment platform - Google Patents
Multi-cell mechanical simulation experiment platform Download PDFInfo
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- CN102759481B CN102759481B CN201210211792.8A CN201210211792A CN102759481B CN 102759481 B CN102759481 B CN 102759481B CN 201210211792 A CN201210211792 A CN 201210211792A CN 102759481 B CN102759481 B CN 102759481B
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Abstract
The invention provides a multi-cell mechanical loading device. The loading device comprises a piston of which the upper end is provided with a brace and a loading box of which one side is provided with an opening, wherein the specification of the piston is consistent with the internal specification of the loading box; the bottom of the loading box is provided with a sensor; and a signal acquisition instrument is arranged outside the loading box. The invention also provides a multi-cell mechanical simulation experiment platform. The experiment platform comprises the multi-cell mechanical loading device as mentioned above, a computer system and a universal material testing machine with a clamp, wherein the multi-cell mechanical loading device is fixed on the clamp; and the computer system is connected with the universal material testing machine. The invention has the following advantages: through the up-and-down movement of the piston, air is compressed to load pressure to cells, the stress uniformity of the cells can be ensured, and the accuracy of the determination result can be improved; the movement of the piston is controlled by the universal material testing machine, the pressure loading process can be exactly simulated, rapid response is achieved, and a complicated pressure change process can be simulated; and the multi-cell mechanical simulation experiment platform has the advantages of simple structure and convenience in operation.
Description
Technical field
The present invention relates to cell biomechanics technical field, specifically, is a kind of cellulous mechanical simulation experiment porch.
Background technology
Cell biomechanics (mechanocytobiology) is the forward position of current life science development, the many aspects that relate to cyto-mechanics research, wherein one of focus be exactly cell because of the caused damage of mechanical force, and about mechanical function cell growth, reformation, mechanotransduction and gene expression so that cause the impact of the processes such as ATP is synthetic.The further investigation of cell biomechanics, not only the research of biomechanics, biomechanical model and the biomaterial etc. of current cell and organizational project, molecular biosciences mechanics and bioengineering, various soft or hard tissues has been played to important facilitation, and for the further developing and break through of life science, will bring into play impact difficult to the appraisal.
Conventional macromechanics loading method and experimental technique cannot directly be used on cell, so find suitable cell loading method and cytomorphosis and relevant Biological measurement means, will be the matter of utmost importance that cell biomechanics faces.Conventional cyto-mechanics experimental technique is divided into unicellular experimental technique and many cells experimental technique, totally can be divided into four classes: 1. adopt microtechnic and micropipette aspiration method directly individual cells to be loaded, and by Microprocessor System for Real Time Record record in addition, utilize image processor and computing machine to carry out displacement measurement and numerical analysis on deformation, its Typical Representative is exactly micropipette aspiration experimental technique; 2. adopting other medium, utilize rheology principle indirectly cell to be loaded, as flow cell, suspension technology, micropore screen method etc., is mainly by fluid shearing force or the pressure producing that flows, cell to be loaded; 3. utilize the base material of cell adherence is loaded, after being loaded, base material make the strain transfer of base material to cell, as two-way strain loading method, gas boosting loading technique and the three-dimensional loading technique etc. of the unidirectional strain loading of four-point bending beam and membrane material; 4. utilize the method that directly cell surface receptor is applied magnetic field distortion power, the variation of measuring cytoskeleton rigidity.
Above-mentioned four conventional class experimental techniques are applicable to separately the cell type that will study and respectively have its relative merits, as do not reflected the physiological status, complex structure, expensive etc. of cell.As Chinese periodical < < China's Tissue Engineering Study and clinical rehabilitation > >, the 13rd volume, the 22nd phase, the article of publishing " development and the application of cell in vitro compressive stress charger ", a kind of cell in vitro compressive stress charger is disclosed, the principle of work of this device is: the cultivation product and the cultured cells thereof that maintain cells in vitro existence and growth are put into cell culture chamber, gases at high pressure in high-pressure gas chamber (steel cylinder) enter in cell culture chamber with stable pressure after two-stage decompression device reduces pressure certain pressure, when the pressure in cell culture chamber reaches predetermined value, close air inlet electromagnetic valve, in cell culture chamber, form stable closed environment.Compressive stress loading section principle, for utilizing branch line stepper motor to drive cylinder pressurization, makes to form in cell culture chamber the periodicity air pressure change of certain frequency, size, thereby makes the cell in cell culture chamber be subject to corresponding action of compressive stress.Test completely, open exhaust solenoid valve, gas is discharged through it.This device can quantitatively be controlled loading size, effect frequency and the acting duration etc. of compressive stress, but there is following defect in this device: (1) cell culture chamber is connected by snorkel with cylinder, gas is finally to spread after entering cell culture chamber by snorkel, and the propelling in the existing dead ahead of diffusion motion of this gas diffusion, also the disperse of oriented surrounding, this just causes cell in cell culture chamber stressed is not that simulated experiment result exists error uniformly, (2) need multi-step pressure reducing apparatus, solenoid valves etc. are controlled gas frequency and air pressure size, the parts running reaction time is long, poor sensitivity, for comparatively complicated Mechanical loading process, be difficult to simulation, " Rolling Dagon method " operational power curve of for example inventing population Ji Feng old docter of TCM shows: the vertical wave-amplitude of Rolling Dagon method is high, ascending branch is precipitous, ascending angle is large, crest is sharp-pointed, the backswing wave-amplitude of decent can be up to 1/3～1/2 of main ripple (forward swing ripple), crest is sharp-pointed, because the pressure size gap between Mechanical loading process complexity and adjacent process is large, thereby use said apparatus will be difficult to accurate simulation.In sum, need a kind of Mechanical loading process of accurately Simulation of Complex badly, pressure-loaded is installed uniformly, but about this class device, have not been reported at present.
Biomaterial pilot system (MTS), claim again universal testing machine, the test of multiaxis biomechanics and sport simulated system, adopt the electronic technology mode that combines with mechanical drive, the measurement of load, distortion, displacement and control are had to higher precision and sensitivity, can carry out the automatic Control experiment of constant speed loading, constant speed distortion, constant speed displacement, and have the function of low all load cycle, distortion circulation, displacement cycle, be widely used in the biomechanics experiment of tissue.It can also export variable pressure as requested, the kinetic curve of the complex pressure change procedures such as simulation massage manipulation, this for develop the Mechanical loading process of above-mentioned accurately Simulation of Complex and pressure-loaded uniformly device provide may, thereby provide possible for the gimmick simulation experiment platform building based on cell biomechanics.
Summary of the invention
The object of the invention is for deficiency of the prior art, a kind of Mechanical loading process, pressure-loaded many cells mechanical loading unit even, simple in structure, easy and simple to handle of accurately Simulation of Complex is provided.
Another object of the present invention is that a kind of cellulous mechanical simulation experiment porch is provided.
For achieving the above object, the technical scheme that the present invention takes is:
A kind of many cells mechanical loading unit, described charger comprises that upper end is provided with the piston of brace and the loading case of an opening, the specification of described piston is consistent with loading case internal specifications, and described loading case bottom is provided with sensor, and outside is provided with signal sampler.
Described piston and loading case are all cylindrical.
Described loading case is to be made by organic glass.
Described sensor is wireless senser.
Described sensor is wired sensor, and described sensor is connected with signal sampler by wire.
For realizing above-mentioned second object, the technical scheme that the present invention takes is:
A kind of cellulous mechanical simulation experiment porch, described experiment porch comprises as above arbitrary described many cells mechanical loading unit, computer system and is provided with the universal testing machine of fixture, described many cells mechanical loading unit is fixed on fixture, and described computer system is connected with universal testing machine.
The invention has the advantages that:
1, many cells mechanical loading unit of the present invention utilizes the pressurized air that moves up and down of piston with to cell on-load pressure, can guarantee that cell is stressed evenly, improves the accuracy of measurement result;
What 2, cellulous mechanical simulation experiment porch of the present invention adopted is the motion of universal testing machine control piston, simulated pressure loading procedure accurately, and being quick on the draw, pressure change procedure that can Simulation of Complex;
3, cellulous mechanical simulation experiment porch of the present invention is simple in structure, and controllability is good, easy and simple to handle.
Accompanying drawing explanation
Accompanying drawing 1 is a kind of many cells mechanical loading unit structural representation of the present invention.
Accompanying drawing 2 is a kind of cellulous mechanical simulation experiment porch structural representations of the present invention.
Accompanying drawing 3 is fourth season peak medium-sized Rolling Dagon method dynamic curve diagrams.
Accompanying drawing 4 is simulation Rolling Dagon method curves.
Accompanying drawing 5 is simulation Rolling Dagon method curve motion tracks.
Accompanying drawing 6 is sinusoidal curve running orbits.
[embodiment]
Below in conjunction with accompanying drawing, embodiment provided by the invention is elaborated.
The Reference numeral and the ingredient that in accompanying drawing, relate to are as follows:
1. load case 2. pistons
3. brace 4. sensors
5. signal sampler 10. many cells mechanical loading units
20. universal testing machine 30. computer systems
6. fixture 61. first fixtures
62. second fixtures
embodiment 1 a kind of many cells mechanical loading unit of the present invention
Please refer to Fig. 1, Fig. 1 is a kind of many cells mechanical loading unit structural representation of the present invention.Described many cells mechanical loading unit is provided with one and loads case 1, and described loading case 1 is made by organic glass, cylindrical, outer dia 13cm, and inside diameter 10cm, high 13cm, the one side of upper end is opening.Described many cells mechanical loading unit is also provided with a piston 2, and described piston 2 is cylindrical, and diameter is identical with the internal diameter that loads case 1, thickness 3cm.The central authorities of the upper end of described piston 2 are provided with brace 3, and described brace 3 is also a right cylinder, by stainless steel material, is made.The bottom of described loading case 1 is provided with through hole (not shown), chimeric sensor 4 closely in described through hole, described sensor 4 is wireless sensers, end face with the bottom surface that loads case 1 on a surface level.The outside of described loading case 1 is also provided with signal sampler 5.Described signal sampler 5 is connected by wireless signal with sensor 4.
embodiment 2 a kind of cellulous mechanical simulation experiment porch of the present invention
Please refer to Fig. 2, Fig. 2 is a kind of cellulous mechanical simulation experiment porch structural representation of the present invention.Described cellulous mechanical simulation experiment porch is provided with many cells mechanical loading unit 10, universal testing machine 20 and computer system 30 as described in Example 1.Described universal testing machine 20Wei American MTS company produces, and model is MTS858, is provided with fixture 6, and described fixture 6 comprises the first fixture 61 and the second fixture 62.Described many cells mechanical loading unit 10 is fixed between the first fixture 61 and the second fixture 62, and brace 3 and the first fixture 61 are fixing, and the bottom and the second fixture 62 that load case 1 are fixing.Described computer system 30 is connected with universal testing machine 20, in described computer system 30, waveform compilation software is installed.
Using method and the principle of work thereof of a kind of cellulous mechanical simulation experiment porch of the present invention are:
1. many cells mechanical loading unit 10 use ultraviolet rays of the present invention are irradiated 1 hour to sterilization;
2. cell is inoculated in to sterile petri dish, adds cell culture fluid;
3. many cells mechanical loading unit 10 of the present invention is fixed on the fixture 6 of universal testing machine 20, opens data collecting instrument 5, regulate zero point, to loading in case 1, put into the double dish that contains cell culture fluid;
4. use universal testing machine 20 to load 700N load, check that loading case 1 has or not gas leakage, whether data collecting instrument 5 operations are normal;
5. utilize the waveform compilation software in computer system 30, editor's simulation mechanics loading curve;
6. sensor 4 induction loading case 1 bottoms are stressed, and by wireless signal, send stress value to signal sampler 5, and the output of regulation and control mechanics, combines waveform and mechanics output, edits out experiment working procedure.
In whole process, along with the variation of the load of exerting pressure, the piston 2 in many cells mechanical loading unit 10 of the present invention moves up and down, by airtight gaseous conductance, pressure load is applied on the cell loading in case 1 bottom double dish, causes cell stressed.
For above-described embodiment 1-2, it should be noted that, many cells mechanical loading unit of the present invention is to adopt piston 2 to loading case 1 internal compression air on-load pressure of the same size with it, and the direction of motion of air is consistent downward, so the suffered pressure of cell is uniform; Because the loading of pressure is to realize by piston 2, and the motion of piston 2 only need be controlled by universal testing machine 20, thus simulated pressure loading procedure accurately, and also can accurately simulate for complicated pressure change procedure; Described loading case 1 and the shape of piston 2 are not limited only to cylindrical, and the two shape is consistent, piston 2 specifications and the identical leakproofness of guaranteeing to load case 1 of loading case 1 internal specifications, as is designed to fillet quadrilateral, but pref. cylindrical, to facilitate modeling; Described loading case 1 adopts organic glass to make, and its inner wall smooth, can fit tightly with piston 2 surroundings, prevents gas leakage; Described piston 2 can be used all-aluminium piston, cast-iron piston, cast-steel piston, aluminum matrix composite piston etc., and the wear-resisting and good seal performance of take is choice criteria; For further guaranteeing to load the sealing of case 1, can be coated with the aseptic glycerine of one deck in the surrounding of piston 2; The end face of described sensor 4 will remain on a surface level with the bottom surface that loads case 1, guarantees that the force value of sensor 4 impressions is identical with cell; Described sensor 4 also can adopt wired sensor, while using wired sensor, between sensor 4 and data collecting instrument 5, by wire, is connected and is transmitted signal; Described universal testing machine 20 is not limited only to embodiment 2, also can use the equipment of other producers, take deformation measurement wide ranges, to be out of shape relative error of indicating value little be choice criteria.
embodiment 3 a kind of many cells mechanical simulation experiment porch simulation Rolling Dagon method curve of the present invention
1, experimental technique
The Rolling Dagon method best power source mathematic(al) parameter (strength 4kg, 120 beats/min of frequencies, time 10min) of screening in clinical trial of take is basic parameter, technical requirement in conjunction with fourth season peak medium-sized Rolling Dagon method dynamic curve diagram (see figure 3) and universal testing machine 20, utilize the waveform compilation program in computer system 30, design and regulate and control the output of many cells mechanical loading unit 10 loading forces, concrete operation step is:
1. the many cells mechanical loading unit 10 after ultraviolet irradiation is fixed on universal testing machine 20, connects the power supply of the sensor 4 that loads case 1 bottom, open data collecting instrument 5, regulate zero point, will contain PBS damping fluid double dish and put into loading case 1 bottom;
2. start universal testing machine 20, operation Basic Test Ware program, displacement protection is set for (10mm,-60mm), actuator direction is downwards negative value, select Auto Offset program by opening Meters window after the zeroing of MTS system deviation, be 0.1 by precision setting in Sensitive option;
3. open hydraulic pressure, select manual control program, handling actuator drives piston 2 to move up and down in loading case 1, numerical value according to data collecting instrument 5 shows, determining the corresponding relation of applied force output and piston 2 displacements, and finally by displacement range designation mechanics, exported, is (100N between the load zones that experiment needs,-660N)/(160N ,-660N);
4. in waveform compilation program, select Axial Displacement and Axial Command as control model, be set as the curve of output longitudinal axis, then in Plot Mode, select Time as curve of output transverse axis and by Auto Offset skew zeroing;
5. according to requirement of experiment, Rolling Dagon method curvilinear correlation parameter/sinusoids parameter is simulated in input, and curve output frequency is 2 hertz, and curve output time is 10 minutes, 1200 circulations of output in waveform compilation program;
6. the curve output error of using the compensation of Pvc correction to program to cause because of factors such as skews, fair curve waveform, removes the enable option in manually controlling, and selects Interlock lock program, starts test;
7. open oscillograph, stable waveform is kept in system, and observe its fitting effect;
8. test finishes rear save routine and curve, and removal many cells mechanical loading unit 10, finally closes hydraulic pressure, exits waveform compilation program.
2, experimental result
2.1 simulation Rolling Dagon method curve results
The Rolling Dagon method curve of simulation as shown in Figure 4.Set universal testing machine maximum pressure output 660N, minimum pressure output 100N, corresponding absolute displacement is demarcated as (43.43mm ,-47.50mm), and simulation Rolling Dagon method curve motion track is as shown in Figure 5.
2.2 sinusoidal curve results
Sinusoidal curve is as the contrast of simulation Rolling Dagon method curve, its universal testing machine maximum pressure output 600N, minimum pressure output 160N, corresponding relative displacement is demarcated as (7.00mm, 7.00mm), 120 beats/min of frequencies, the duration is 10 minutes, curve motion track is as shown in Figure 6.
From the above results, can find out, many cells mechanical simulation experiment porch of the present invention is simulated pressure loading procedure accurately.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the inventive method; can also make some improvement and supplement, these improvement and supplement and also should be considered as protection scope of the present invention.
Claims (5)
1. a many cells mechanical loading unit, it is characterized in that, described charger comprises that upper end is provided with the piston of brace and the loading case of an opening, described piston and loading case are all cylindrical, described piston bush is connected on the inside of described loading case, the specification of described piston is consistent with loading case internal specifications, and described piston up-down pressurized air is with the cell on-load pressure to loading case bottom; Described loading case bottom is provided with sensor, and outside is provided with signal sampler.
2. many cells mechanical loading unit according to claim 1, is characterized in that, described loading case is to be made by organic glass.
3. many cells mechanical loading unit according to claim 1 and 2, is characterized in that, described sensor is wireless senser.
4. many cells mechanical loading unit according to claim 1 and 2, is characterized in that, described sensor is wired sensor, and described sensor is connected with signal sampler by wire.
5. a cellulous mechanical simulation experiment porch, it is characterized in that, described experiment porch comprises many cells mechanical loading unit as claimed in claim 1 or 2, computer system and is provided with the universal testing machine of fixture, described many cells mechanical loading unit is fixed on fixture, and described computer system is connected with universal testing machine.
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| FR3064359B1 (en) | 2017-03-21 | 2021-04-16 | Centre Nat Rech Scient | DEVICE FOR THE MECHANICAL CHARACTERIZATION OF AN ELEMENT OF INTEREST, FOR EXAMPLE AN OVOCYTE |
| CN109706079B (en) * | 2018-12-11 | 2022-03-25 | 江苏理工学院 | Flow cavity for simulating flowing environment behind bracket |
| CN111057640A (en) * | 2019-12-09 | 2020-04-24 | 西北工业大学 | Experimental device for in-situ research cell mechanical characteristics |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1834220A (en) * | 2005-07-07 | 2006-09-20 | 重庆大学 | Microstress applicator for in vitro cell |
| CN101092595A (en) * | 2006-06-23 | 2007-12-26 | 上海交通大学医学院附属第九人民医院 | Experimental apparatus for loading cell through digital controlled mechanical strain |
| CN101717719A (en) * | 2009-12-08 | 2010-06-02 | 四川大学 | In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof |
| CN201648396U (en) * | 2009-12-08 | 2010-11-24 | 四川大学 | In-vitro cellular pressure loading device |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1834220A (en) * | 2005-07-07 | 2006-09-20 | 重庆大学 | Microstress applicator for in vitro cell |
| CN101092595A (en) * | 2006-06-23 | 2007-12-26 | 上海交通大学医学院附属第九人民医院 | Experimental apparatus for loading cell through digital controlled mechanical strain |
| CN101717719A (en) * | 2009-12-08 | 2010-06-02 | 四川大学 | In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof |
| CN201648396U (en) * | 2009-12-08 | 2010-11-24 | 四川大学 | In-vitro cellular pressure loading device |
Non-Patent Citations (1)
| Title |
|---|
| 何辉.细胞力学实验方法研究：压力加载及改进的膜式张应变加载.《中国优秀硕士学位论文全文数据库 医药卫生科技辑》.2005, * |
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