CN207632784U - A kind of multi-functional cell blowpipe - Google Patents

A kind of multi-functional cell blowpipe Download PDF

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Publication number
CN207632784U
CN207632784U CN201721734892.3U CN201721734892U CN207632784U CN 207632784 U CN207632784 U CN 207632784U CN 201721734892 U CN201721734892 U CN 201721734892U CN 207632784 U CN207632784 U CN 207632784U
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CN
China
Prior art keywords
blowpipe
ontology
wall
connecting tube
cell
Prior art date
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Expired - Fee Related
Application number
CN201721734892.3U
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Chinese (zh)
Inventor
程天才
李冉
姚伦广
胡小敏
尹延震
张振杰
刘阳坤
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Nanyang Normal University
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Nanyang Normal University
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Priority to CN201721734892.3U priority Critical patent/CN207632784U/en
Application granted granted Critical
Publication of CN207632784U publication Critical patent/CN207632784U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

A kind of multi-functional cell blowpipe, the cell blowpipe includes blowpipe ontology and rubber head, the cell blowpipe further includes the connecting tube of screen pipe, suction nozzle and plastic material, it is axially arranged with the spongy layer of multiple superposed along it inside screen pipe, the circumferential surface for filtering pipe outer wall is equipped with external screw thread, the upper end inner wall of blowpipe ontology is equipped with the internal thread for being socketed in filtering pipe outer wall, so that the internal thread that blowpipe ontology is arranged by the upper end is connect with filtering pipe thread seal, suction nozzle is located at the lower end of blowpipe ontology;Rubber head is fixedly connected with one end of connecting tube, and the inner wall of the other end of connecting tube is equipped with the internal thread for being socketed in filtering pipe outer wall, so that connecting tube is passed through the internal thread that its inner wall is arranged and is connect with filtering pipe thread seal.Connecting tube and screen pipe are connected by screw thread, and screen pipe is connected by screw thread, and air-tightness is good;It is axially arranged with the spongy layer of multiple superposed along it inside screen pipe, the anti-suck in liquid relief;It is more convenient and not easy to pollute when blowing cell.

Description

A kind of multi-functional cell blowpipe
Technical field
The utility model is related to Bioexperiment utensils, and in particular to a kind of multi-functional cell blowpipe.
Background technology
In genetic engineering experiment, various albumen, virus usually are produced with cell, or be used for basic research.In cell It needs to use culture bottle and cell blowpipe in culture, because some cells are adherent growths, in culture and transfer, needs handle Cell is blown down from wall.Existing common Tissue Culture Flask specification is in 25 ~ 225cm2, it is existing test use liquid-transfering gun, bar The utensils such as family name's suction pipe, pipette, it is difficult to it is applicable in the culture bottle of different size, and in transfer liquid, it is understood that there may be partially liq Reflux;Meanwhile in experimental implementation, need repeatedly to be blown and beaten against adherent or half adherent cell, attract due to hand-held Pipe is operated, and suction tube may be caused to fall off because of improper operation, or after so that suction tube is contacted with human hands, then with The phenomenon that culture bottle wall surface contacts and causes experimental pollution.
Utility model content
The purpose of this utility model, which is to provide one kind, can not only realize cell piping and druming function, additionally it is possible to realize that cell liquid turns The multi-functional cell blowpipe moved.
The utility model technical solution used for the above purpose is:A kind of multi-functional cell blowpipe, the cell Blowpipe includes blowpipe ontology and rubber head, which further includes the connecting tube of screen pipe, suction nozzle and plastic material, screen pipe Inside is axially arranged with the spongy layer of multiple superposed along it, and the circumferential surface for filtering pipe outer wall is equipped with external screw thread, the upper end of blowpipe ontology Inner wall is equipped with the internal thread for being socketed in filtering pipe outer wall, the internal thread for making blowpipe ontology be arranged by the upper end and filtering Pipe thread seal connects, and suction nozzle is located at the lower end of blowpipe ontology;The rubber head is fixedly connected with one end of connecting tube, makes connection Pipe is connected to the inner cavity of rubber head, and the inner wall of the other end of connecting tube is equipped with the internal thread for being socketed in filtering pipe outer wall, makes The internal thread that connecting tube is arranged by its inner wall is connect with filtering pipe thread seal.
Wherein, the outer wall of blowpipe ontology is arranged with end cap, and the center of end cap upper surface is equipped with and blowpipe ontology clearance fit Through-hole;The inner wall circumferential surface of end cap outer rim is equipped with the internal thread for the bottleneck that can be connected to Tissue Culture Flask, is located at outside through-hole The upper surface of the end cap of circle is fixedly connected with the bellows of flexible and transparent material, and the upper end of bellows is fixed on connection Guan Shang.
Wherein, the end cap is rounded, is located at the end cap outside bellows and is equipped with and Tissue Culture Flask inner cavity The air hole of perforation, and the inner wall circumferential surface of through-hole is equipped with rubber ring.
Preferably, the central axis of bellows, rubber head, connecting tube, screen pipe and blowpipe ontology overlaps setting.
In the utility model, blowpipe ontology is the hollow body of glass material, and the outer wall of blowpipe ontology is equipped with graduation mark, and The outer diameter of blowpipe ontology is identical as the outer diameter of connecting tube.
In the utility model, suction nozzle is fixedly connected into integral mechanism with blowpipe ontology, and suction nozzle is obliquely installed, in suction nozzle Angle between mandrel line and the central axis of blowpipe ontology is 120 ~ 150 °.
In the utility model, the setting direction of the port of suction nozzle lower end is arranged with the centerline axis parallel of blowpipe ontology.
In the utility model, the length of blowpipe ontology is 10cm, 15cm, 20cm or 25cm, is respectively suitable for different size Culture bottle.
Advantageous effect:The connecting tube and screen pipe being connect with rubber head are connected by screw thread, and screen pipe is connected by screw thread, gas Close property is good;It is axially arranged with the spongy layer of multiple superposed along it inside screen pipe, the anti-suck in liquid relief;More when blowing cell It is convenient and not easy to pollute.
In addition, the outer wall of blowpipe ontology is arranged with the end cap that can be connected on Tissue Culture Flask, blowpipe ontology is made to stop When only operating, it can be suspended in Tissue Culture Flask;In operation, by mobile blowpipe ontology, bellows follow blowpipe Ontology telescopic moving realizes that cell blows and beats function.
Wherein, bellows limit the motion range of blowpipe ontology, prevent the blowpipe caused by hand takes off power Ontology falls to the risk in Tissue Culture Flask;And the blowpipe ontology that hand can be avoided to contact is stretched into Tissue Culture Flask and is made At pollution risk.
Description of the drawings
Fig. 1 is the schematic diagram of the utility model;
Fig. 2 is the seperated schematic diagram of Fig. 1;
Fig. 3 is the implementation figure of the utility model.
Reference numeral:1, blowpipe ontology, 2, rubber head, 3, connecting tube, 4, screen pipe, 5, suction nozzle, 6, external screw thread, 7, interior spiral shell Line, 8, end cap, 9, through-hole, 10, bellows, 11, air hole, 12, Tissue Culture Flask, 13, spongy layer.
Specific implementation mode
To make technological means of the utility model, creation characteristic and the purpose reached be easy to understand, with reference to The utility model is expanded on further in schematic diagram, but the utility model range claimed is not limited to specific embodiment party Range described in formula.
A kind of multi-functional cell blowpipe, as depicted in figs. 1 and 2, the cell blowpipe include blowpipe ontology 1 and rubber head 2, this is thin Born of the same parents' blowpipe further includes the connecting tube 3 of screen pipe 4, suction nozzle 5 and plastic material, is axially arranged with multi-laminate along it inside screen pipe 4 The spongy layer 13 set, suction nozzle 5 are located at the lower end of blowpipe ontology 1.
Wherein, rubber head 2 is spherical in shape, and rubber head 2 is fixedly connected with one end of connecting tube 3, makes the inner cavity of connecting tube 3 and rubber head 2 Connection, the inner wall of the other end of connecting tube 3 are equipped with the internal thread for being socketed on 4 outer wall of screen pipe, connecting tube 3 are made to pass through it The internal thread of inner wall setting is connect with 4 thread seal of screen pipe;The circumferential surface of 4 outer wall of screen pipe is equipped with external screw thread 6, blowpipe ontology 1 upper end inner wall is equipped with the internal thread for being socketed on 4 outer wall of screen pipe, and blowpipe ontology 1 is made to pass through in the setting of the upper end Screw thread is connect with 4 thread seal of screen pipe.
Preferably, it is additionally provided with sealing ring between blowpipe ontology 1 and screen pipe 4, between connecting tube 3 and screen pipe 4.
Preferably, rubber head 2 and connecting tube 3 are structure as a whole, and blowpipe ontology 1 and suction nozzle 5 are structure as a whole.
Preferably, blowpipe ontology 1 is the hollow body of glass material, and the outer wall of blowpipe ontology 1 is equipped with graduation mark, and blowpipe The outer diameter of ontology 1 is identical as the outer diameter of connecting tube 3.
As shown in Figure 1, suction nozzle 5 is fixedly connected into integral mechanism with blowpipe ontology 1, and suction nozzle 5 is obliquely installed, suction nozzle 5 Angle β between central axis and the central axis of blowpipe ontology 1 is 120 ~ 150 °.
As shown in Figure 1, the setting direction of the port of 5 lower end of suction nozzle and the centerline axis parallel of blowpipe ontology 1 are arranged.
In the utility model, the length of blowpipe ontology 1 could be provided as 10cm, 15cm, 20cm or 25cm, to adapt to difference The Tissue Culture Flask of size.
Wherein, as shown in figure 3, the outer wall of blowpipe ontology 1 is arranged with end cap 8, the center of 8 upper surface of end cap is equipped with and blowpipe The through-hole 9 of 1 clearance fit of ontology;The inner wall circumferential surface of 8 outer rim of end cap is equipped with the bottleneck that can be connected to Tissue Culture Flask 12 Internal thread is fixedly connected with the bellows 10 of flexible and transparent material, ripple positioned at the upper surface of the end cap 8 of 9 outer ring of through-hole The upper end of telescoping tube 10 is fixed on the outer wall of connecting tube 3.
Preferably, end cap 8 is rounded, is located at the end cap 8 outside bellows 10 and is equipped with and Tissue Culture Flask 12 The air hole 11 of inner cavity perforation, and the inner wall circumferential surface of through-hole 9 is equipped with rubber ring.
Preferably, the central axis coincidence of bellows 10, rubber head 2, connecting tube 3, screen pipe 4 and blowpipe ontology 1 is set It sets.
Preferably, the angle β between the central axis of suction nozzle 5 and the central axis of blowpipe ontology 1 is 135 °, under suction nozzle 5 The setting direction of the port at end is arranged with the centerline axis parallel of blowpipe ontology 1, and such setting keeps the port of suction nozzle larger, Piping and druming area can be expanded;The intrinsic liquid of blowpipe can also be made to easily flow out, resistance reduces, and avoids generating more bubbles Foam.
When being blown and beaten, blowpipe ontology is obliquely installed, and makes the setting direction of the port of suction nozzle lower end be in piping and druming plane Certain angle also avoids the generation of foam to a certain extent.
When imbibition:First the air in rubber head is emptied, then suction nozzle is stretched into liquid level or less imbibition, rubber head is used for negative pressure absorbing Liquid.
When blowing and beating cell:Tissue Culture Flask is placed on the table, the end cap of the utility model is passed through into helicitic texture It is connected to the bottleneck of Tissue Culture Flask, pinches rubber head with thumb and index finger, another hand can buttress blowpipe ontology, be stretched to ripple The position of the blowpipe ontology of draw limitation and angle are adjusted, and the cell bottle face of the suction nozzle Cell-oriented attaching of bending passes through It repeatedly extrudes rubber head and draws and blow out liquid, complete suspension cell operation;Wherein, liquid being blown out along wall as possible, operation is soft, Utmostly avoid the generation of foam.
In the prior art, the bottleneck of Tissue Culture Flask is obliquely installed relative to its body, is to realize that working as cell trains When foster bottle is kept flat, all positions of cell blowpipe or the accessible culture bottom surface of cell scraper facilitate harvest cell, avoid cultivating Bottle generates the extraction dead angle for being not easy to extract cell.
In order to adapt to the bottleneck that it is obliquely installed, bottle cap seals the bottle cap of the Tissue Culture Flask of the prior art with whorl of bottleneck Afterwards, bottle cap is also obliquely installed, i.e. the center of bottle cap and the center of bottleneck coincides.For this purpose, the shape of the end cap of the utility model It is identical as the shape and size of encapsulated cell culture bottle are used in the prior art with size, the bellows being fixed on end cap It is vertically arranged with the upper surface of end cap, and the central axis of bellows overlaps setting with the central axis of end cap.
In the utility model, can also blowpipe ontology be set to the material of clear hard plastics, to reduce cost;And plastics Material is lighter, easily controllable.
The utility model is suitable for batch combination production, and a rubber head can be adapted to the blowpipe ontology of different lengths specification, And cleaning and sterilizing is carried out to blowpipe ontology, it can reuse, reduce experimental cost.
In the utility model, it is that air can be discharged by squeezing rubber head that rubber head, which uses silica gel material, main function, makes glue Head is internal to form negative pressure, to realize the absorption to liquid.
The blowpipe ontology of the utility model could be provided as the hollow cylinder of latus rectum, may be set to be from top to bottom (I.e. from rubber head to the direction of suction nozzle)The diminishing hollow body of internal diameter, graduation mark is arranged outside in blowpipe ontology, when drawing liquid, It can realize quantitative control.
In the utility model, connecting tube and screen pipe are connected by screw thread, and screen pipe is connected by screw thread, can be certain In range, the length of entire cell blowpipe is adjusted;It can also be individually replaced this component after some parts damages.
Other not described contents of the utility model belong to the prior art.
Above example makes in order to illustrate the technical solution of the utility model, and the purpose is to be to make those skilled in the art The content of the utility model can be understood and be practiced, but the scope of protection of the utility model is not limited with this.It is every according to The equivalent changes or modifications made according to the substantive content of the utility model, should all cover in the scope of protection of the utility model It is interior.

Claims (8)

1. a kind of multi-functional cell blowpipe, the cell blowpipe include blowpipe ontology and rubber head, it is characterised in that:The cell blowpipe is also Include the connecting tube of screen pipe, suction nozzle and plastic material, is axially arranged with the spongy layer of multiple superposed, mistake along it inside screen pipe The circumferential surface of chimney filter outer wall is equipped with external screw thread, and the upper end inner wall of blowpipe ontology is equipped with the interior spiral shell for being socketed in filtering pipe outer wall Line makes the internal thread that blowpipe ontology is arranged by the upper end be connect with filtering pipe thread seal, and suction nozzle is located under blowpipe ontology End;
The rubber head is fixedly connected with one end of connecting tube, and connecting tube is made to be connected to the inner cavity of rubber head, the other end of connecting tube Inner wall be equipped with for be socketed in filtering pipe outer wall on internal thread, so that connecting tube is passed through the internal thread and filtering that its inner wall is arranged Pipe thread seal connects.
2. a kind of multi-functional cell blowpipe according to claim 1, it is characterised in that:The outer wall of blowpipe ontology is arranged with end Lid, the center of end cap upper surface are equipped with the through-hole with blowpipe ontology clearance fit;The inner wall circumferential surface of end cap outer rim is equipped with can It is connected to the internal thread of the bottleneck of Tissue Culture Flask, flexible and transparent material is fixedly connected with positioned at the upper surface of the end cap of through-hole outer ring The upper end of the bellows of matter, bellows is fixed in connecting tube.
3. a kind of multi-functional cell blowpipe according to claim 2, it is characterised in that:The end cap is rounded, is located at End cap outside bellows is equipped with the air hole penetrated through with Tissue Culture Flask inner cavity, and the inner wall circumferential surface of through-hole is equipped with Rubber ring.
4. a kind of multi-functional cell blowpipe according to claim 2 or 3, it is characterised in that:Bellows, rubber head, company It takes over, the central axis coincidence setting of screen pipe and blowpipe ontology.
5. a kind of multi-functional cell blowpipe according to claim 1, it is characterised in that:Blowpipe ontology is the sky of glass material The outer wall of heart body, blowpipe ontology is equipped with graduation mark, and the outer diameter of blowpipe ontology is identical as the outer diameter of connecting tube.
6. a kind of multi-functional cell blowpipe according to claim 1, it is characterised in that:Suction nozzle is fixedly connected with blowpipe ontology Integral mechanism, and suction nozzle is obliquely installed, the angle between the central axis and the central axis of blowpipe ontology of suction nozzle is 120 ~ 150°。
7. a kind of multi-functional cell blowpipe according to claim 1 or 6, it is characterised in that:The port of suction nozzle lower end is set It sets direction and the centerline axis parallel of blowpipe ontology is arranged.
8. a kind of multi-functional cell blowpipe according to claim 1, it is characterised in that:The length of blowpipe ontology be 10cm, 15cm, 20cm or 25cm.
CN201721734892.3U 2017-12-13 2017-12-13 A kind of multi-functional cell blowpipe Expired - Fee Related CN207632784U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201721734892.3U CN207632784U (en) 2017-12-13 2017-12-13 A kind of multi-functional cell blowpipe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201721734892.3U CN207632784U (en) 2017-12-13 2017-12-13 A kind of multi-functional cell blowpipe

Publications (1)

Publication Number Publication Date
CN207632784U true CN207632784U (en) 2018-07-20

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201721734892.3U Expired - Fee Related CN207632784U (en) 2017-12-13 2017-12-13 A kind of multi-functional cell blowpipe

Country Status (1)

Country Link
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111925914A (en) * 2020-09-16 2020-11-13 天津长风中济高新技术有限公司 Monocyte blowing and beating analyzer with adjustable blowing and beating force

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111925914A (en) * 2020-09-16 2020-11-13 天津长风中济高新技术有限公司 Monocyte blowing and beating analyzer with adjustable blowing and beating force

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180720

Termination date: 20181213