CN207619424U - A kind of doughnut slipstream piece-rate system - Google Patents
A kind of doughnut slipstream piece-rate system Download PDFInfo
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- CN207619424U CN207619424U CN201721446805.4U CN201721446805U CN207619424U CN 207619424 U CN207619424 U CN 207619424U CN 201721446805 U CN201721446805 U CN 201721446805U CN 207619424 U CN207619424 U CN 207619424U
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- doughnut
- hollow
- slipstream
- piece
- pipeline
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Abstract
The utility model discloses a kind of doughnut slipstream piece-rate systems that can be reused, by accessing CIP（Incumbent firms）Water source or SIP（Sterilizing in place）Vapour source realizes the cleaning and sterilizing of hollow-fiber module and entire pipeline, realizes apparatus assembly Reusability, greatly reduce production cost, the trouble for also eliminating dismounting and change component repeatedly simultaneously, simplifies process, thus greatly reduces and generates pollution to system due to operation error.
Description
Technical field
The utility model is related in biological cell culture technique separation of solid and liquid more particularly to a kind of doughnut slipstream
Piece-rate system.
Background technology
Common Zooblast cultivation method has batch cultivation, feed batch fermentation, perfusion culture etc., wherein perfusion is trained
It is foster that the culture medium in system can be made to be continuously updated, meet the needs of cell is to nutriment in time, while removing lactic acid, ammonium etc.
Toxic Metabolites give the growing environment that cell is more excellent and stablizes.This method be effectively improved zooblast density and
Motility rate extends the incubation time of cell, and protein yield is substantially increased.Being focused primarily upon using early stage for the method is adherent
The fixed bed culture of cell.The perfusion culture of suspension cell, the requirement to cell retention equipment is very high, common rotating filter
Device, inclination settler etc. are unable to effectively catching cell, lead to be applied or are retained based on hollow fiber conduit currently on the market
The tangential flow filtration system of principle design, but be disposable equipment, cost is too high, cannot meet most of medicines
The demand of factory.
Utility model content
The purpose of this utility model is to provide one kind to reuse, full-automatic CIP（Incumbent firms）、SIP（In place
Sterilizing）Doughnut slipstream piece-rate system.It is applicable not only to suspension cell perfusion culture, the dense of cell can also be applied to
It contracts and detaches, the purifying etc. of destination protein.
The utility model adopts the following technical solution realizes：
A kind of doughnut slipstream piece-rate system has liquid feeding end component, magnetic suspension water pump, hollow by piping connection successively
Fiber module, outlet end component；
The hollow-fiber module side wall upper and lower side be equipped with trepanning, upper end trepanning by pipeline be connected to sepage collection assembly and
CIP/SIP valve groups；Lower end trepanning is connected to draining valve group by pipeline.
The sepage collection assembly is made of peristaltic pump and pipe valve component；
Pipeline between the hollow-fiber module and outlet end component is equipped with counterbalance valve and flowmeter；
Preferably, the hollow-fiber module is connected to film integrality detection components by pipeline；
Preferably, the film integrality detection components are made of gas control valve, filter and valve.
Preferably, it is equipped with temperature sensor at the filter, sepage collection assembly draining valve group and magnetic suspension water pump；
Pipeline between the hollow-fiber module and outlet end component is equipped with pressure sensor, the doughnut group
Pipeline between part and magnetic suspension water pump is equipped with pressure sensor, and pressure sensor is equipped in the hollow-fiber module.
The occupation mode of the utility model is as follows：
Liquid feeding end component is connected by the liquid outlet or sample tap of connection valve and culture tank, and outlet end component passes through connection
Valve and the liquid return hole of culture tank connect；SIP intake valves on filter are connect with high-temperature steam source.
The connection valve of liquid feeding end component and outlet end component remains off, and opens SIP intake valves, is conveyed into pipeline
High-temperature steam sterilizes, and by adjusting SIP intake valves, steam pressure pressure is no more than 1.05Bar in control piper, made
Temperature at filter module, sepage collection assembly and magnetic suspension water pump reaches 121 DEG C, kept for 30 minutes or more, complete sterilizing work
Make.
It is then shut off the valve of SIP intake valves and other each nodes, opens the connection of liquid feeding end component and outlet end component
Valve between valve and sepage collection assembly and hollow-fiber module, opens magnetic suspension water pump and peristaltic pump, liquid in culture tank
Body is entered under the action of magnetic suspension water pump by liquid feeding end component, by hollow-fiber module, by cell retention in doughnut
In pipe and it is fed back to culture tank, not celliferous liquid medium is oozed out from doughnut, and collection is pumped out by peristaltic pump.
The advantageous effects of the utility model are：The utility model provides a kind of doughnut that can be reused
Slipstream piece-rate system, by accessing CIP（Incumbent firms）Water source or SIP（Sterilizing in place）Vapour source realizes doughnut
The cleaning and sterilizing of component and entire pipeline realize apparatus assembly Reusability, greatly reduce production cost, while also saving
The trouble of dismounting and change component repeatedly, simplifies process, thus greatly reduces and generates pollution to system due to operation error.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model.
Specific implementation mode
By the following description of the embodiment, public understanding the utility model will more be contributed to, but can't should
Specific embodiment given by applicant is considered as the limitation to technical solutions of the utility model, it is any special to component or technology
The definition of sign be changed and/or to overall structure make form and immaterial convert the technology of the utility model of being regarded as
Protection domain defined by scheme.
The present embodiment provides a kind of doughnut slipstream piece-rate systems, and structure by piping connection as shown in Figure 1, had successively
Liquid feeding end component 1, magnetic suspension water pump 2, hollow-fiber module 3, outlet end component 4；
3 side wall upper and lower side of the hollow-fiber module is equipped with trepanning, and upper end trepanning is connected to sepage collection assembly 5 by pipeline
With CIP/SIP valve groups 10；Lower end trepanning is connected to draining valve group 9 by pipeline.
The sepage collection assembly 5 is made of peristaltic pump 5.1 and pipe valve component 5.2；
Pipeline between the hollow-fiber module 3 and outlet end component 4 is equipped with counterbalance valve 7 and flowmeter 8；
The hollow-fiber module 3 is connected to film integrality detection components 6 by pipeline；
The film integrality detection components 6 by gas control valve 6.1, filter 6.2 and valve group 6.3 at.
It is equipped with temperature sensor TC1 at the filter 6.2, temperature sensor is equipped at the sepage collection assembly 5
TC2 is equipped with temperature sensor TC3 at the draining valve group 9, temperature sensor TC4 is equipped at the magnetic suspension water pump 2；
Pipeline between the hollow-fiber module 3 and outlet end component 4 is described hollow equipped with pressure sensor PE3
Pipeline between fiber module 3 and magnetic suspension water pump 2 is equipped with pressure sensor PE1, and pressure is equipped in the hollow-fiber module
Force snesor PE2.
The occupation mode of the utility model is as follows：
Liquid feeding end component is connected by the liquid outlet or sample tap of connection valve and culture tank, and outlet end component passes through connection
Valve and the liquid return hole of culture tank connect；SIP intake valves on filter are connect with high-temperature steam source.
The connection valve of liquid feeding end component and outlet end component remains off, and opens SIP intake valves, is conveyed into pipeline
High-temperature steam sterilizes, and by adjusting SIP intake valves, steam pressure pressure is no more than 1.05Bar in control piper, made
Temperature at filter module, sepage collection assembly and magnetic suspension water pump reaches 121 DEG C, kept for 30 minutes or more, complete sterilizing work
Make.
It is then shut off the valve of SIP intake valves and other each nodes, opens the connection of liquid feeding end component and outlet end component
Valve between valve and sepage collection assembly and hollow-fiber module, opens magnetic suspension water pump and peristaltic pump, liquid in culture tank
Body is entered under the action of magnetic suspension water pump by liquid feeding end component, by hollow-fiber module, by cell retention in doughnut
In pipe and it is fed back to culture tank, not celliferous liquid medium is oozed out from doughnut, and collection is pumped out by peristaltic pump.
The advantageous effects of the utility model are：The utility model provides a kind of doughnut that can be reused
Slipstream piece-rate system, by accessing CIP（Incumbent firms）Water source or SIP（Sterilizing in place）Vapour source realizes doughnut
The cleaning and sterilizing of component and entire pipeline realize apparatus assembly Reusability, greatly reduce production cost, while also saving
The trouble of dismounting and change component repeatedly, simplifies process, thus greatly reduces and generates pollution to system due to operation error.
Certainly, the utility model can also have other various embodiments, without departing substantially from the spirit of the present invention and its essence
In the case of, those skilled in the art can make various corresponding change and deformations, but this according to the utility model
A little corresponding change and deformations should all belong to the protection domain of the utility model the attached claims.
Claims (6)
1. a kind of doughnut slipstream piece-rate system has liquid feeding end component, magnetic suspension water pump, hollow fibre by piping connection successively
Tie up component, outlet end component, it is characterised in that：
The hollow-fiber module side wall upper and lower side is equipped with trepanning, and upper end trepanning is connected to sepage collection assembly and CIP/ by pipeline
SIP valve groups；Lower end trepanning is connected to draining valve group by pipeline；
The sepage collection assembly is made of peristaltic pump and pipe valve component.
2. a kind of doughnut slipstream piece-rate system according to claim 1, it is characterized in that：The hollow-fiber module
Pipeline between outlet end component is equipped with counterbalance valve and flowmeter.
3. a kind of doughnut slipstream piece-rate system according to claim 1, it is characterized in that：The sepage collection assembly
It is equipped with temperature sensor at draining valve group and magnetic suspension water pump.
4. a kind of doughnut slipstream piece-rate system according to claim 1, it is characterized in that：
Pipeline between the hollow-fiber module and outlet end component is equipped with pressure sensor, the hollow-fiber module with
Pipeline between magnetic suspension water pump is equipped with pressure sensor, and pressure sensor is equipped in the hollow-fiber module.
5. a kind of doughnut slipstream piece-rate system according to claim 1, it is characterized in that：The hollow-fiber module
It is connected to film integrality detection components by pipeline；The film integrality detection components are by gas control valve, filter and valve group
At.
6. a kind of doughnut slipstream piece-rate system according to claim 5, it is characterized in that：It is equipped at the filter
Temperature sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721446805.4U CN207619424U (en) | 2017-11-02 | 2017-11-02 | A kind of doughnut slipstream piece-rate system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721446805.4U CN207619424U (en) | 2017-11-02 | 2017-11-02 | A kind of doughnut slipstream piece-rate system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207619424U true CN207619424U (en) | 2018-07-17 |
Family
ID=62827725
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721446805.4U Active CN207619424U (en) | 2017-11-02 | 2017-11-02 | A kind of doughnut slipstream piece-rate system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207619424U (en) |
-
2017
- 2017-11-02 CN CN201721446805.4U patent/CN207619424U/en active Active
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