CN207591335U - A kind of device for preventing cell fragment from blocking purification column - Google Patents
A kind of device for preventing cell fragment from blocking purification column Download PDFInfo
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- CN207591335U CN207591335U CN201721676805.3U CN201721676805U CN207591335U CN 207591335 U CN207591335 U CN 207591335U CN 201721676805 U CN201721676805 U CN 201721676805U CN 207591335 U CN207591335 U CN 207591335U
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Abstract
The utility model provides a kind of purifying column device for preventing cell fragment from blocking, including main purification column, multiple pre-columns and main purifying column sleeve, multiple pre-columns are connected in parallel on the upstream of main purification column, purifying filler is equipped in main purification column and pre-column, reversal valve is equipped in the upstream of pre-column, the entrance of reversal valve is connected with sprue, the outlet of reversal valve is respectively connected to the entrance of multiple pre-columns, the outlet of main purification column is connected with collection device, it is used for intercepting cell fragment by the purification column for the identical filler for pre-installing a small size before purification column, so that for separation purification column then avoid it is blocked, it is blocked relative to the purification column of processing large volume, the processing that pre-column blocks is then easy to be more, and by setting multiple pre-columns, improve the efficiency of purifying.
Description
Technical field
The utility model is related to a kind of devices for preventing cell fragment from blocking purification column.
Background technology
As the development of modern biotechnology and the application of protein engineering are more and more extensive, Protein purification techniques also become
It obtains and is increasingly taken seriously.Cell fermentation product is by centrifugation, treated that supernatant is direct during protein purification
It needs to carry out a series of pre-treatment when crossing purification column to remove the clogging that can may generate purification column, and locates
It is higher to manage this time cost occupied that blocks, is found in purification process, the top section of blocked always purification column.
Invention content
For above deficiency, the utility model provides a kind of device for preventing cell fragment from blocking purification column, required use
Technical solution be,
A kind of purifying column device for preventing cell fragment from blocking, which is characterized in that including main purification column, multiple pre-columns and master
Column sleeve is purified, multiple pre-columns are connected in parallel on the upstream of main purification column, purifying filler are equipped in the main purification column and pre-column,
The upstream of pre-column is equipped with reversal valve, and the entrance of reversal valve is connected with sprue, and the outlet of reversal valve is respectively connected to multiple pre-columns
Entrance, the outlet of main purification column is connected with collection device；It is pure that main purification column includes the first purification section, the second purification section and third
Change section, the first purified of filling in the first purification section, the second purified of filling in the second purification section, filling the in third purification section
Three purifieds, the purification capacity of the first purification section, the second purification section and third purification section are strengthened successively；The main purifying column sleeve peace
On the outside of main purification column, main purifying column sleeve includes first set section, second set of section, third set section, the 4th set of section and the 5th set of section,
And first set section, second set of section, third set section, the 4th set of section and the 5th set of section are each provided with transparent saturating window, each cover Duan Jun
It can independently be rotated relative to main purification column, the first set section is located at the periphery of the first purification section, and it is pure that second set of section is located at first
Change the periphery of the intersection of section and the second purification section, third set section is located at the periphery of the second purification section, the 4th set of section is located at second
The periphery of the intersection of purification section and third purification section, the 5th set of section are located at the periphery of third purification section.
It is also needed while using above technical scheme using further technical solution,
The reversal valve is three position four-way directional control valve, and is controlled by electric control gear that the electric control gear includes flow
Detector, feedback circuit and cylinder are equipped with flow detector in the tail portion of pre-column, when underfed is worked as in flow detector detection
When, electric signal can be passed to cylinder by feedback circuit, and cylinder pushes reversal valve to move on another station, pre- using another
Column carries out preliminary purification to liquid.
The quantity at least two of the pre-column, in some preferred modes, the quantity of pre-column can be according to purification column
Spatial arrangement be designed.
The volume of the pre-column is much smaller than the volume of main purification column, the purification capacity of the filler in pre-column from top to bottom one
It causes, in some preferred modes, it is for the ease of cleaning and replacing installation that the volume of pre-column, which is less than main purification column,.
The beneficial effects of the utility model, the purification column that the identical filler of a small size is pre-installed before purification column are used for blocking
Cut cell fragment, be used for after this small size pre-column plays a role the purification column that detaches then avoid it is blocked, relative to place
The purification column for managing large volume blocks, and the processing that pre-column blocks is then easy to be more, and by setting multiple pre-columns, improves purifying
Efficiency.
Description of the drawings
Fig. 1 is the pictorial diagram of the present apparatus.
Fig. 2 is the schematic diagram of the present apparatus.
Fig. 3 is the automatically controlled figure of the present apparatus.
Fig. 4 is main purifying column sleeve.
Reference numeral：Three position four-way directional control valve 1, pre-column 2, pre- column packing 3, main purification column 4, third purified 5, second
Purified 6, the first purified 7, first set section 8, second set of section 9, third set section 10, the 4th set of section 11, the 5th set of section 12.
Specific embodiment
The present apparatus is described further below in conjunction with the accompanying drawings.
As shown in Figs 1-4, a kind of device for preventing cell fragment from blocking purification column, which is characterized in that including pre-column 2 and master
Purification column 4, the main purification column 4 are internally provided with filler, and filler is divided into third purified 5, and the second purified 6, first is pure
Compound 7, the granular size of filler once reduce, and are equipped with pre-column 2 in the front end of main purification column, pre-column 2 is internally provided with pre-column and fills out
Object 3 is filled, and the granular size of pre- column packing 3 is consistent with the granular size of the leading portion filler 7 of column purification column, in pre-column 2
Front end is equipped with three position four-way directional control valve 1, and three position four-way directional control valve 1 is connected with sprue, the tail end and collection device of main purification column 1
Connection.
The reversal valve is three position four-way directional control valve 1, and is controlled by electric control gear, and the electric control gear includes flow
Detector, feedback circuit and cylinder are equipped with flow detector in the tail portion of pre-column 2, when underfed is worked as in flow detector detection
When, electric signal can be passed to cylinder by feedback circuit, and cylinder pushes reversal valve to move on another station, pre- using another
Column carries out preliminary purification to liquid.
The quantity at least two of the pre-column 2, in some preferred modes, the quantity of pre-column 2 can be according to purifying
The spatial arrangement of column is designed.
The volume of the pre-column 2 is much smaller than the volume of main purification column 4, the particle of pre- column packing 3 size one from top to bottom
It causes, in some preferred modes, it is for the ease of cleaning and replacing installation that the volume of pre-column, which is less than main purification column,.
It is additionally provided with main purifying column sleeve on the outside of the main purification column, main purifying column sleeve includes 8, second sets of sections 9 of first set section, the
Three sets of sections 10, the 4th set of section 11 and the 5th set of section 12, and 8, second sets of sections 9 of first set section, third set section 10, the 4th set of 11 and of section
5th set 12 sections are equipped with transparent saturating window, can independently be relatively rotated between set section, and the first set section 8 corresponds to the first purification section 7,
Second set of section 9 corresponds to the intersection of the first purification section 7 and the second purification section 6, third set section 10 corresponds to the second purification section the 6, the 4th
The intersection that section 11 corresponds to the second purification section 6 and third purification section 5 is covered, the 5th set of section 12 corresponds to third purification section 5.
The utility model principle, in purification process, the liquid that pre-column flows into sprue is purified, takes out bulky grain
Liquid flows into main purification column by the flow detector of pre-column tail portion after impurity, when flow detector detects underfed,
Electric signal can be passed to cylinder by feedback circuit, and cylinder pushes reversal valve to move on another station, using another pre-column
Preliminary purification is carried out to liquid.
Although there is employed herein reference numeral in figure, the possibility using other terms is not precluded, uses these arts
Language is construed to other any one just for the sake of more easily the essence of the present invention is explained and described, them and adds
Restrictive condition is all disagreed with spirit of the present invention.
Claims (6)
1. a kind of device for preventing cell fragment from blocking purification column, which is characterized in that pure including main purification column, multiple pre-columns and master
Change column sleeve, multiple pre-columns are connected in parallel on the upstream of main purification column, purifying filler are equipped in the main purification column and pre-column, pre-
The upstream of column is equipped with reversal valve, and the entrance of reversal valve is connected with sprue, and the outlet of reversal valve is respectively connected to multiple pre-columns
Entrance, the outlet of main purification column are connected with collection device；Main purification column includes the first purification section, the second purification section and third purifying
Section, the first purification section the first purified of interior filling, the second purification section is interior to fill the second purified, and third is filled in third purification section
Purified, the purification capacity of the first purification section, the second purification section and third purification section are strengthened successively；The main purifying column sleeve installation
On the outside of main purification column, main purifying column sleeve includes first set section, second set of section, third set section, the 4th set of section and the 5th set of section, and
First set section, second set of section, third set section, the 4th set of section and the 5th set of section are each provided with transparent saturating window, each cover Duan Junneng
Independent to be rotated relative to main purification column, the first set section is located at the periphery of the first purification section, and second set of section is located at the first purifying
It is pure positioned at second that the periphery of the intersection of section and the second purification section, third set section are located at the periphery of the second purification section, the 4th set of section
Change the periphery of the intersection of section and third purification section, the 5th set of section is located at the periphery of third purification section.
A kind of 2. device for preventing cell fragment from blocking purification column according to claim 1, which is characterized in that the commutation
Valve is three position four-way directional control valve.
3. a kind of device for preventing cell fragment from blocking purification column according to claim 2, which is characterized in that described three
Four-way reversing valve is equipped with electric control gear.
A kind of 4. device for preventing cell fragment from blocking purification column according to claim 1, which is characterized in that the pre-column
Quantity at least two.
A kind of 5. device for preventing cell fragment from blocking purification column according to claim 1, which is characterized in that the pre-column
Volume be much smaller than main purification column volume.
A kind of 6. device for preventing cell fragment from blocking purification column according to claim 5, which is characterized in that the pre-column
The purification capacity of interior filler is consistent from top to bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721676805.3U CN207591335U (en) | 2017-12-05 | 2017-12-05 | A kind of device for preventing cell fragment from blocking purification column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721676805.3U CN207591335U (en) | 2017-12-05 | 2017-12-05 | A kind of device for preventing cell fragment from blocking purification column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207591335U true CN207591335U (en) | 2018-07-10 |
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ID=62762698
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721676805.3U Active CN207591335U (en) | 2017-12-05 | 2017-12-05 | A kind of device for preventing cell fragment from blocking purification column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207591335U (en) |
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2017
- 2017-12-05 CN CN201721676805.3U patent/CN207591335U/en active Active
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