CN207570882U - Double-face dyeing device - Google Patents
Double-face dyeing device Download PDFInfo
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- CN207570882U CN207570882U CN201721382367.XU CN201721382367U CN207570882U CN 207570882 U CN207570882 U CN 207570882U CN 201721382367 U CN201721382367 U CN 201721382367U CN 207570882 U CN207570882 U CN 207570882U
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- Prior art keywords
- transparent carrier
- slot
- double
- dyeing device
- face dyeing
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- 238000004043 dyeing Methods 0.000 title claims abstract description 31
- 239000011521 glass Substances 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 238000009826 distribution Methods 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 238000004040 coloring Methods 0.000 abstract description 3
- 239000000427 antigen Substances 0.000 description 8
- 102000036639 antigens Human genes 0.000 description 8
- 108091007433 antigens Proteins 0.000 description 8
- 238000010586 diagram Methods 0.000 description 4
- 238000011532 immunohistochemical staining Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000975 dye Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 238000003364 immunohistochemistry Methods 0.000 description 2
- 238000000608 laser ablation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000012309 immunohistochemistry technique Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of double-face dyeing device, including stent, the first transparent carrier and the second transparent carrier；Stent is equipped with the first slot and the second slot for being respectively used to be inserted into for the first transparent carrier and the second transparent carrier, the first transparent carrier it is parallel after being inserted into the first slot and the second slot respectively with the second transparent carrier and between with gap；First transparent carrier has along the perforative micropore of its thickness direction.The double-face dyeing device on the first transparent carrier by setting micropore, when by histotomy when samples are fixed on first transparent carrier, sample in micropore can all contact the detection reagents such as antibody with two sides, so as to double-face dyeing, obtained coloring and detection signal are significantly increased and enhance compared with traditional single-face dyeing.
Description
Technical field
The utility model is related to immunohistochemistry/immunofluorescence dyeing device field, more particularly, to a kind of two-sided dye
Color device.
Background technology
Immunohistochemistry technique belongs to not in fact in life science using very extensive, but to obtain satisfied coloration result
Easily, even also tending to be difficult to the professional of molecular biology experiment experience for many years, trace it to its cause mainly because
It is very high to antibody specificity and binding force requirement for immunohistochemical staining, and pass through fixed tissue antigen and often resist with corresponding
Binding force between body falls sharply, and causes coloration result undesirable.Traditional immunohistochemical staining is all that single side is used on glass slide
(tissue free surface) antibody is hatched and the method for dyeing, and the limitation of this method is that antigen is combined with antibody and is not enough, therefore,
Coloration result is unsatisfactory.
Utility model content
Based on this, it is necessary to be combined asking of being not enough with antibody for antigen during traditional immunohistochemical staining
Topic, provides a kind of double-face dyeing device that can improve coloring.
A kind of double-face dyeing device, including stent, the first transparent carrier and the second transparent carrier；The stent is equipped with
It is respectively used to the first slot and the second slot that are inserted into for first transparent carrier and second transparent carrier, described first
Transparent carrier it is parallel after being inserted into first slot and second slot respectively with second transparent carrier and between have
Gap；First transparent carrier has along the perforative micropore of its thickness direction.
The quantity of micropore is multiple on first transparent carrier in one of the embodiments,.
Multiple micropores are in array distribution at the middle part of first transparent carrier in one of the embodiments,.
The pore diameter range of the micropore is 5 μm~500 μm in one of the embodiments,.
First transparent carrier is coverslip or rigid plastics with multi-cellular structure in one of the embodiments,
Film.
The thickness of first transparent carrier is less than the thickness of second transparent carrier in one of the embodiments,.
Second transparent carrier is glass slide in one of the embodiments,.
The distance between first slot and second slot are 0.5mm~5mm in one of the embodiments,.
The distance between first slot and second slot are 1mm~2mm in one of the embodiments,.
The stent includes fixed part and connecting portion in one of the embodiments, and there are two the fixed parts, two
The fixed part is oppositely arranged, and each fixed part is equipped with first slot and the second slot, two fixations in inside
Portion is connected in the side close to second slot by the connecting portion.
Above-mentioned double-face dyeing device is fixed on by setting micropore on the first transparent carrier when by samples such as histotomies
When on first transparent carrier, can the surface of first transparent carrier and the first transparent carrier and the second transparent carrier it
Between add in antibody, the detection reagents such as dye liquor, so as to which the sample in micropore can all contact the detection reagents such as antibody with two sides, from
And can be with double-face dyeing, obtained coloring and detection signal are significantly increased and enhance compared with traditional single-face dyeing.
Description of the drawings
Fig. 1 is the assembling schematic diagram of the double-face dyeing device of an embodiment；
Fig. 2 is the structure diagram of stent in Fig. 1；
Fig. 3 is the structure diagram of the first transparent carrier in Fig. 1；
Fig. 4 is the structure diagram of the second transparent carrier in Fig. 1.
Specific embodiment
For the ease of understanding the utility model, the utility model is more fully retouched below with reference to relevant drawings
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can in many different forms come in fact
It is existing, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments is the public affairs made to the utility model
Open the understanding more thorough and comprehensive of content.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with belonging to the technology of the utility model
The normally understood meaning of technical staff in domain is identical.It is only in the term used in the description of the utility model herein
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" includes
The arbitrary and all combination of one or more relevant Listed Items.
As shown in Figure 1, the double-face dyeing device 10 of an embodiment includes stent 100, the first transparent carrier 200 and the
Two transparent carriers 300.First transparent carrier 200 and the second transparent carrier 300 can be mounted on stent 100.
Specifically, incorporated by reference to Fig. 1 and Fig. 2, in the present embodiment, stent 100 is transparent for first equipped with being respectively used to
The first slot 102 and the second slot 104 that 200 and second transparent carrier 300 of carrier is inserted into.First transparent carrier 200 and second
Transparent carrier 300 be inserted into respectively it is parallel after the first slot 102 and the second slot 104 and between there is gap.
In a more specific embodiment, stent 100 includes fixed part 110 and connecting portion 120.Fixed part 110 has
Two, two fixed parts 110 are oppositely arranged.Each fixed part 110 is equipped with above-mentioned first slot, 102 and second slot 104 in inside.
The first slot 102 on two fixed parts 110 is oppositely arranged, and the second slot 104 on two fixed parts 110 is oppositely arranged.
First slot 102 and the second slot 104 extend along the length direction of stent 100.Connecting portion 120 is plate-like structure, and two solid
Determine portion 110 to connect by connecting portion 120 in the side close to the second slot 104.
Further, in one embodiment, it is also associated with baffle between the end of two fixed parts 110.Two fixed parts
110th, a connecting portion 120 and baffle fit are formed in the rectangle frame of one end open and upper opening, open-ended to be used to supply
First transparent carrier 200 and the second transparent carrier 300 are inserted into, and upper opening is used for for detecting instrument observation detection.
Heat safe plastic production molding can be used in stent 100.
Incorporated by reference to Fig. 1 and Fig. 3, in the present embodiment, the first transparent carrier 200 has along the perforative micropore of its thickness direction
202.First transparent carrier 200 is used for the samples such as fixed tissue slices, by opening up micropore 202 on the first transparent carrier 200,
Sample in micropore 202 can carry out two side reactions and detection, be conducive to improve the signal strength of testing result, Jin Erti
The accuracy of high detection result.
In one embodiment, the quantity of micropore 202 is multiple on the first transparent carrier 200.Preferably, multiple micropores
202 in array distribution at the middle part of the first transparent carrier 200.
In one embodiment, the pore diameter range of micropore 202 is 5 μm~500 μm, such as 200 μm, can regard specific sample
It is adjusted.The mode that laser ablation may be used in micropore 202 is formed.
In one embodiment, the first transparent carrier 202 be coverslip or rigid plastics film with multi-cellular structure,
Such as PEN plastic films.Preferably, the first transparent carrier 202 has the coverslip of multi-cellular structure.
Incorporated by reference to Fig. 1, Fig. 3 and Fig. 4, in one embodiment, the thickness of the first transparent carrier 200 is less than the second transparent load
The thickness of body 300.Preferably, the second transparent carrier 300 can directly select traditional glass slide.By way of laser ablation
Micropore 202 is formed on traditional coverslip, and as new sample carrier, since cover-glass thickness is relatively thin, compared with thickness compared with
It is etched on thick traditional glass slide, process is more simple and practicable, and cost of manufacture is relatively low, and is more conducive to carry out double-face dyeing detection.
In one embodiment, the distance between the first slot 102 and the second slot 104 for 0.5mm~5mm namely are worked as
After first transparent carrier 200 and the second transparent carrier 300 are inserted into the first slot 102 and the second slot 104 respectively, the first transparent load
There is the gap of 0.5mm~5mm between 200 and second transparent carrier 300 of body.Preferably, the first slot 102 and the second slot
The distance between 104 be 1mm~2mm.In other embodiments, the distance between the first slot 102 and the second slot 104 may be used also
Specifically to be adjusted depending on specific detected sample.
The specific embodiment of tissue section strain detection is carried out using above-mentioned double-face dyeing device 10 the following provide one.
Embodiment 1
Major experimental step is similar to traditional immunohistochemistry of histotomy and immunofluorescence dyeing method, makees herein
Brief description, is as follows：
1. dewax aquation：Histotomy is fished for above-mentioned first transparent carrier 200 to dry, and dimethylbenzene and ladder are put into after roasting piece
Spend dewaxing and aquation in alcohol；
2. antigen retrieval：After the first transparent carrier 200 for sample being fixed with is dewaxed in clear water rinse a period of time, adds
Enter 3%H2O210min is impregnated, so as to remove endogenic catalase, then outwells H2O2, washed twice in clear water, then add
Enter antigen retrieval buffer solution and be heated to 100 DEG C, maintain 20 minutes, the site of antigen is made fully to be exposed；
3. site is closed：Buffer solution is outwelled, in addition serum, is closed some nonspecific sites, 37 DEG C half
Hour；First transparent carrier, 200 and second transparent carrier 300 is inserted into stent 100；
4. add primary antibody：In the surface of the first transparent carrier 200 and the first transparent carrier 200 and the second transparent carrier 300
Between be added dropwise target antigen specific antibody, 4 DEG C overnight.
5. add fluorescence secondary antibody：Double-face dyeing device 10 from refrigerator is taken out, is put into PBS and washes 3 times, each 5min, is wiped
After PBS around stem organization, in the surface of the first transparent carrier 200 and the first transparent carrier 200 and the second transparent carrier 300
Between plus fluorescence secondary antibody, be placed in half an hour in 37 DEG C of incubators.
8. nuclear targeting：After the slice, thin piece after fluorescent staining is rinsed a period of time with clear water, it is soaked in DAPI and dyes,
General animal tissue is 5~15 minutes.
Above-mentioned double-face dyeing device 10 on the first transparent carrier 200 by setting micropore 202, when by samples such as histotomies
When product are fixed on first transparent carrier 200, the sample in micropore 202 can all contact the detections such as antibody examination with two sides
Agent considerably increases the chance that antigen is combined with antibody, and immunohistochemical staining can be made to obtain ideal effect color, obtained inspection
Signal is surveyed to significantly increase compared with traditional single-face dyeing.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Embodiment described above only expresses the several embodiments of the utility model, and description is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that the common skill for this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (10)
1. a kind of double-face dyeing device, which is characterized in that including stent, the first transparent carrier and the second transparent carrier；It is described
Stent is equipped with the first slot and second for being respectively used to be inserted into second transparent carrier for first transparent carrier and inserts
Slot, first transparent carrier are parallel after being inserted into first slot and second slot respectively with second transparent carrier
There is gap between and；First transparent carrier has along the perforative micropore of its thickness direction.
2. double-face dyeing device as described in claim 1, which is characterized in that the quantity of micropore is on first transparent carrier
It is multiple.
3. double-face dyeing device as claimed in claim 2, which is characterized in that multiple micropores are in array distribution described the
The middle part of one transparent carrier.
4. double-face dyeing device as described in claim 1, which is characterized in that the pore diameter range of the micropore is 5 μm~500 μ
m。
5. double-face dyeing device as described in claim 1, which is characterized in that first transparent carrier is with more micropore knots
The coverslip of structure or rigid plastics film.
6. such as double-face dyeing device according to any one of claims 1 to 5, which is characterized in that first transparent carrier
Thickness is less than the thickness of second transparent carrier.
7. double-face dyeing device as claimed in claim 6, which is characterized in that second transparent carrier is glass slide.
8. such as double-face dyeing device according to any one of claims 1 to 5, which is characterized in that first slot with it is described
The distance between second slot is 0.5mm~5mm.
9. double-face dyeing device as claimed in claim 8, which is characterized in that between first slot and second slot
Distance be 1mm~2mm.
10. such as double-face dyeing device according to any one of claims 1 to 5, which is characterized in that the stent includes fixed part
And connecting portion, there are two the fixed parts, and two fixed parts are oppositely arranged, and each fixed part is equipped with described in inside
First slot and the second slot, two fixed parts are connected in the side close to second slot by the connecting portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721382367.XU CN207570882U (en) | 2017-10-23 | 2017-10-23 | Double-face dyeing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721382367.XU CN207570882U (en) | 2017-10-23 | 2017-10-23 | Double-face dyeing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207570882U true CN207570882U (en) | 2018-07-03 |
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ID=62696248
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721382367.XU Active CN207570882U (en) | 2017-10-23 | 2017-10-23 | Double-face dyeing device |
Country Status (1)
| Country | Link |
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| CN (1) | CN207570882U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114279795A (en) * | 2020-09-28 | 2022-04-05 | 成都天士力诺唯生物科技有限公司 | Rapid detection system, detection method and application of tissue sample |
-
2017
- 2017-10-23 CN CN201721382367.XU patent/CN207570882U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114279795A (en) * | 2020-09-28 | 2022-04-05 | 成都天士力诺唯生物科技有限公司 | Rapid detection system, detection method and application of tissue sample |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210902 Address after: 510000 room 801, No. 49, Shuiyin Siheng Road, Tianhe District, Guangzhou City, Guangdong Province Patentee after: Pan Yonghong Address before: 510530 No. 79 Ruihe Road, Luogang District, Guangzhou City, Guangdong Province Patentee before: GUANGZHOU JI AO BIOTECHNOLOGY Co.,Ltd. |