CN207552348U - A kind of cell culture vector and cell culture apparatus - Google Patents
A kind of cell culture vector and cell culture apparatus Download PDFInfo
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- CN207552348U CN207552348U CN201721426692.1U CN201721426692U CN207552348U CN 207552348 U CN207552348 U CN 207552348U CN 201721426692 U CN201721426692 U CN 201721426692U CN 207552348 U CN207552348 U CN 207552348U
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- cell culture
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Abstract
The utility model provides a kind of cell culture vector and cell culture apparatus, and including multiple hinges, the hinge is rounded, and multiple hinges are set from top to bottom, and multiple hinges are attached along welded rails.Cell culture vector can reduce the influence of other ingredients or materials onto cells growth；Manufacture, the connection mode of carrier are specified, reduces influence of the unknown ingredient to culture；It reduces in incubation and the physical shear of cell is injured；There is certain supporting role, increase mass transfer, pass oxygen function.Cell culture apparatus can be used in attached cell culture, while provide more operating position, while bottom magnetic force agitating paddle, reduce to the shearing force of cell when being inoculated with, so as to reduce the damage to cell.
Description
Technical field
The utility model is related to technical field of cell culture, are filled more particularly to a kind of cell culture vector and cell culture
It puts.
Background technology
Current chip carrier (foreign countries), is supported by skeleton, and framework ingredient and main material are different, during cell culture
Dissolution be precipitated it is more.Current chip carrier (country) has corner angle in shape, and when cultivating in the reactor, cell shearing is made
With relatively by force, it is unfavorable for the growth of cell.Current chip carrier connection mode is indefinite.
Meanwhile current cell culture apparatus (spinner), there are top stirring and middle part stirring, machinery during cell inoculation
Movement is larger to the shearing force of cell, has more cell that can be damaged.Current cell culture apparatus (spinner), is used for
The suspension culture of mammalian cell, it is impossible to be used in adherent or adhere-wall culture effect is poor.Current cell culture apparatus
(spinner), station is less during cell culture, is not suitable for cell Combined culture and data monitoring in a device.
Utility model content
In view of the foregoing deficiencies of prior art, the purpose of this utility model is to provide a kind of cell culture vector and
Cell culture apparatus, it is stronger for solving chip carrier cell shearing effect in the prior art, it is unfavorable for the growth of cell, connects
Mode is indefinite；Meanwhile cell culture apparatus shearing force is larger, has more cell that can be damaged, it is impossible to be used in it is adherent or
Adhere-wall culture effect is poor, and station is less during cell culture, and being not suitable for cell, Combined culture and data monitoring are asked in a device
Topic.
The utility model provides a kind of cell culture vector, and including multiple hinges, the hinge is rounded, multiple pages
Piece is set from top to bottom, and multiple hinges are welded along welded rails.
In an embodiment of the utility model, the direct welding manner of hinge is ultrasonic bonding, high frequency welds
It connects, heat seal welding or laser welding.
In an embodiment of the utility model, the quantity of the hinge is 2 to 20.
In an embodiment of the utility model, a diameter of 3-100mm of the hinge.
In an embodiment of the utility model, the thickness of the hinge is 0.1-5mm.
In an embodiment of the utility model, the material of the hinge is polyester fiber and/or polypropylene.
The utility model also provides a kind of cell culture apparatus, including above-mentioned cell culture vector and reactor；It is described
Reactor includes magnetic stirring apparatus and multiple culture tanks；The magnetic stirring apparatus is equipped with multiple stations, and the culture tank corresponds to
It is arranged on the station；The cell culture vector is arranged in the culture tank；The culture tank includes tank body, magnetic force stirs
Mix paddle and guiding device；The magnetic agitation paddle is arranged on the bottom of the tank body, and the guiding device is erected at the tank body
Middle part.
In an embodiment of the utility model, the guiding device includes isolation cover and two sieves, the sieve point
It is not located at the upper and lower ends of the isolation cover, multiple mesh are offered on the sieve.
In an embodiment of the utility model, the station quantity is 1-50.
As described above, a kind of cell culture vector and cell culture apparatus of the utility model, have the advantages that：
Cell culture vector can reduce the influence of other ingredients or materials onto cells growth；Specify the manufacturer of carrier
Formula, connection mode reduce influence of the unknown ingredient to culture；It reduces in incubation and the physical shear of cell is injured；Have one
Fixed supporting role increases mass transfer, passes oxygen function.Cell culture apparatus can be used in attached cell culture, while provide more
Operating position, while bottom magnetic force agitating paddle, to the shearing force of cell when reducing inoculation, so as to reduce the damage to cell.
Description of the drawings
Fig. 1 is shown as the structure diagram of cell culture vector in the utility model.
Fig. 2 is shown as the structure diagram of cell culture apparatus in the utility model.
Fig. 3 is shown as the structure diagram of magnetic stirring apparatus in Fig. 2.
Fig. 4 is shown as the structure diagram of culture tank in Fig. 2.
Fig. 5 is shown as the schematic diagram of the flow direction in a device of liquid in Fig. 2.
Component label instructions：
1 cell culture vector
2 reactors
3 magnetic stirring apparatus
4 culture tanks
41 tank bodies
42 magnetic agitation paddles
43 guiding devices
Specific embodiment
Illustrate the embodiment of the utility model below by way of specific specific example, those skilled in the art can be by this theory
Content disclosed by bright book understands other advantages and effect of the utility model easily.The utility model can also be by addition
Different specific embodiments are embodied or practiced, and the various details in this specification can also be based on different viewpoints with answering
With carrying out various modifications or alterations under the spirit without departing from the utility model.It should be noted that in situation about not conflicting
Under, the feature in following embodiment and embodiment can be combined with each other.
It should be noted that the diagram provided in following embodiment only illustrates the basic of the utility model in a schematic way
Conception, component count, shape when only display is with related component in the utility model rather than according to actual implementation in schema then
And size is drawn, kenel, quantity and the ratio of each component can be a kind of random change during actual implementation, and its assembly layout
Kenel may also be increasingly complex.
Referring to Fig. 1 to Fig. 5, it should however be clear that structure, ratio, size depicted in this specification institute accompanying drawings etc., only matching
The revealed content of specification is closed, so that those skilled in the art understands and reads, being not limited to the utility model can
The qualifications of implementation, therefore do not have technical essential meaning, the tune of the modification of any structure, the change of proportionate relationship or size
It is whole, in the case where not influencing the effect of the utility model can be generated and the purpose that can reach, should all still fall in the utility model institute
The technology contents of announcement are obtained in the range of covering.Meanwhile in this specification it is cited as " on ", " under ", "left", "right",
The term of " centre " and " one " etc. is merely convenient to understanding rather than to limit the enforceable range of the utility model for narration,
Its relativeness is altered or modified, in the case where changing technology contents without essence, when being also considered as the enforceable scope of the utility model.
As shown in Figure 1, Fig. 1 is shown as the structure diagram of cell culture vector in the utility model.The utility model carries
For a kind of cell culture vector, including multiple hinges, hinge is rounded, and multiple hinges are set from top to bottom, the weldering of multiple hinges edges
It integrates with and is welded.Using identical material but the hinge of identical material is not limited only to, such as explicitly to cell growth without having
The material of evil effect reduces the influence of other ingredients or materials onto cells growth；Meanwhile it can be reduced using circular hinge
The physical shear of cell is injured in incubation；There is certain supporting role after welding, increase mass transfer, pass oxygen function.
In a specific embodiments, the quantity of hinge is a piece of.Directly can voluntarily it be unfolded in culture solution.
In an embodiment of the utility model, the quantity of hinge is 2 to 20.
Further, a diameter of 3-100mm of hinge.The thickness of hinge is 0.1-5mm.
In general, the material of hinge is polyester fiber and/or polypropylene.May be polyester fiber and polyacrylic combination.
The combination of certain polyester fiber and other materials for meeting the utility model demand is also among the scope of protection of the utility model.
Preferably, the direct welding manner of hinge is ultrasonic bonding, high frequency welding, heat seal welding or laser welding.
Manufacture, the connection mode of carrier are specified, reduces influence of the unknown ingredient to culture；Have after welding certain
Supporting role increases mass transfer, passes oxygen function.In welding, several layers of raw material can be used to carry out ultrasonic bonding by welding machine
Or after heat seal welding, by perforating press punch forming, form required support shapes.
As shown in Figures 2 to 5, Fig. 2 is shown as the structure diagram of cell culture apparatus in the utility model.Fig. 3 is shown
Structure diagram for magnetic stirring apparatus in Fig. 2.Fig. 4 is shown as the structure diagram of culture tank in Fig. 2.Fig. 5 is shown as Fig. 2
The schematic diagram of middle liquid flow direction in a device.The utility model additionally provides a kind of cell culture apparatus, including above-mentioned
Cell culture vector 1 and reactor 2；The reactor 2 includes magnetic stirring apparatus 3 and multiple culture tanks 4；The magnetic stirring apparatus
3 are equipped with multiple stations, and the culture tank 4 is correspondingly arranged on the station；The cell culture vector 1 is arranged on the training
It supports in tank 4；The culture tank 4 includes tank body 41, magnetic agitation paddle 42 and guiding device 43；The magnetic agitation paddle 42 is set
In the bottom of the tank body 41, the guiding device 43 is erected at the middle part of the tank body 41.Further, the guiding device
43 include isolation cover and two sieves, and the sieve is located at the upper and lower ends of the isolation cover, is opened up on the sieve respectively
There are multiple mesh.In general, station quantity is 1-50.
The magnetic agitation paddle 42 of 4 bottom of culture tank can be the abundant mixing of liquid in tank body 41, be in cell culture, pass
Matter, heat transfer and biography oxygen so that liquid each section uniform component.
During cell inoculation, when cell is by cell culture vector 1, it is attached on cell culture vector 1.
In the utility model, when bottom magnetic agitation, magnetic stirring apparatus 3 is stirred by magnetic force and the magnetic force of 41 bottom of tank body
Paddle 42 is mixed to act on.This magnetic stirring apparatus 3 provides multiple operating positions, in use, can be simultaneously to the data of all working position
It is monitored, and then can accurately control the cell culture on an operating position, so as to the function of reaching monitoring and accurately control.
In cell culture, magnetic stirring apparatus 3 is power and monitors and controls device, by adjusting magnetic stirring apparatus 3
Relevant parameter makes magnetic stirring apparatus 3 that magnetic agitation paddle 42 be driven to rotate, the liquid circulation in 42 driving device of magnetic agitation paddle,
So as to make each uniform component in liquid, the mode of liquid circulation flowing is as shown in Figure 5.During inoculating cell, cell from top into
Enter in device, first position is collected where carrier under the action of cycle, and then adherent.So that cell culture apparatus can
It is cultivated for attached cell, while provides more operating position, while bottom magnetic force agitating paddle 42, to cell when reducing inoculation
Shearing force, so as to reduce the damage to cell.
In conclusion the cell culture vector and cell culture apparatus of the utility model, cell culture vector can be reduced
Other ingredients or the influence of materials onto cells growth；Manufacture, the connection mode of carrier are specified, reduces unknown ingredient to training
Foster influence；It reduces in incubation and the physical shear of cell is injured；There is certain supporting role, increase mass transfer, pass oxygen work(
Energy.Cell culture apparatus can be used in attached cell culture, while provide more operating position, while bottom magnetic agitation
Paddle reduces to the shearing force of cell when being inoculated with, so as to reduce the damage to cell.So the utility model effectively overcomes now
There is the various shortcoming in technology and have high industrial utilization.
The above embodiments are only illustrative of the principle and efficacy of the utility model, new not for this practicality is limited
Type.Any person skilled in the art can all carry out above-described embodiment under the spirit and scope without prejudice to the utility model
Modifications and changes.Therefore, such as those of ordinary skill in the art without departing from the revealed essence of the utility model
All equivalent modifications completed under refreshing and technological thought or change, should be covered by the claim of the utility model.
Claims (9)
1. a kind of cell culture vector, which is characterized in that including multiple hinges, the hinge is rounded, and multiple hinges are certainly
Upper and lower setting, multiple hinges are attached along welded rails.
2. cell culture vector according to claim 1, which is characterized in that the direct welding manner of hinge is ultrasound
Wave soldering connects, high frequency welds, heat seal welding or laser welding.
3. cell culture vector according to claim 1, which is characterized in that the quantity of the hinge is 2 to 20.
4. cell culture vector according to claim 1, which is characterized in that a diameter of 3-100mm of the hinge.
5. cell culture vector according to claim 1, which is characterized in that the thickness of the hinge is 0.1-5mm.
6. cell culture vector according to claim 1, which is characterized in that the material of the hinge for polyester fiber and/
Or polypropylene.
7. a kind of cell culture apparatus, which is characterized in that including claim 1 to 6 any one of them cell culture vector and
Reactor；The reactor includes magnetic stirring apparatus and multiple culture tanks；
The magnetic stirring apparatus is equipped with multiple stations, and the culture tank is correspondingly arranged on the station；The cell culture
Carrier is arranged in the culture tank；
The culture tank includes tank body, magnetic agitation paddle and guiding device；The magnetic agitation paddle is arranged on the bottom of the tank body
Portion, the guiding device are erected at the middle part of the tank body.
8. cell culture apparatus according to claim 7, which is characterized in that the guiding device includes isolation cover and two
Sieve, the sieve are located at the upper and lower ends of the isolation cover, multiple mesh are offered on the sieve respectively.
9. cell culture apparatus according to claim 7, which is characterized in that the station quantity is 1-50.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721426692.1U CN207552348U (en) | 2017-10-31 | 2017-10-31 | A kind of cell culture vector and cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721426692.1U CN207552348U (en) | 2017-10-31 | 2017-10-31 | A kind of cell culture vector and cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207552348U true CN207552348U (en) | 2018-06-29 |
Family
ID=62681180
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721426692.1U Active CN207552348U (en) | 2017-10-31 | 2017-10-31 | A kind of cell culture vector and cell culture apparatus |
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| CN (1) | CN207552348U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363680A (en) * | 2018-12-26 | 2020-07-03 | 浙江大学 | Pulse stirring type bioreactor for secretion, separation and collection of exosome |
| CN113637585A (en) * | 2021-08-11 | 2021-11-12 | 四川大学 | High-efficient cell culture reactor and mixing system thereof |
-
2017
- 2017-10-31 CN CN201721426692.1U patent/CN207552348U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363680A (en) * | 2018-12-26 | 2020-07-03 | 浙江大学 | Pulse stirring type bioreactor for secretion, separation and collection of exosome |
| CN111363680B (en) * | 2018-12-26 | 2023-10-13 | 浙江大学 | Pulse stirring type bioreactor for secretion, separation and collection of exosomes |
| CN113637585A (en) * | 2021-08-11 | 2021-11-12 | 四川大学 | High-efficient cell culture reactor and mixing system thereof |
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