CN207537461U - A kind of multiprobe hybridized slides - Google Patents
A kind of multiprobe hybridized slides Download PDFInfo
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- CN207537461U CN207537461U CN201721483366.4U CN201721483366U CN207537461U CN 207537461 U CN207537461 U CN 207537461U CN 201721483366 U CN201721483366 U CN 201721483366U CN 207537461 U CN207537461 U CN 207537461U
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Abstract
The utility model patent belongs to fluorescence in situ hybridization technique field, and in particular to a kind of multiprobe hybridized slides.The multiprobe hybridized slides adhere to slide by probe and sample attachment slide forms；The probe attachment slide is made of detecting probe information tag slot, probe adhering zone and hybridized slides bonding land, is attached in the form of being lyophilized on probe attachment region comprising several mutually independent probe attachment regions, probe in the probe adhering zone；The sample attachment slide is made of sample message recording areas, sample adhering zone and sample isolated area, and the sample adhering zone is made of several mutually independent sample attachment regions.Compared to traditional glass slide, the utility model can on a hybridized slides preset 8 groups of probes simultaneously, and the detection of a variety of probes can be completed on same hybridized slides, enormously simplify the operating procedure of traditional FISH.
Description
Technical field
The utility model patent belongs to fluorescence in situ hybridization technique field, and in particular to a kind of multiprobe hybridized slides.
Background technology
Fluorescence in situ hybridization be it is a kind of grow up in the 1980s radioactive in situ hybridization technical foundation one
Kind on-radiation molecular genetic techniques, a kind of new original position for marking substitution labelled with radioisotope with fluorescein and being formed are miscellaneous
Friendship method.FISH has many advantages, such as safety, quick, high sensitivity and shows multiple color simultaneously.
At present in the detection of hematology FISH, usually used is all conventional slide, and probe and glass slide are
It is separately used alone, it usually needs first using blood sample film-making, then slide is subjected to a series of pre-treatments, then probe is added dropwise
And rubber glue mounting is used, the co-variation of probe sample and hybridization are carried out again after mounting；Blood disease such as leukaemia common at present
FISH detection projects up to tens kinds of number, such as CLL (chronic myelocytic leukemia) needs to detect cMYC gene breaks, P16
Missing, E2A fractures, hyperdiploid detection (CEP10/CEP17/CEP4), TEL/AML1 fusions, MLL fractures, BCR/ABL fusions
And disease type and process are assessed in IGH fractures etc..If with existing hybridization technique for needing to detect a variety of probes
It needs to repeat repeated detection during disease, it is time-consuming and laborious.In addition traditional FISH experiments use traditional FISH probe to need
Want overnight hybridization and disposably detect multiple projects not only to take, and high expense also greatly increases the economy of patient and bears
Load.
Invention content
The utility model is in view of the deficiencies of the prior art, and it is an object of the present invention to provide a kind of multiprobe hybridized slides.
For achieving the above object, the technical solution adopted in the utility model is：
A kind of multiprobe hybridized slides, which is characterized in that the multiprobe hybridized slides are to adhere to slide and sample by probe
This attachment slide two parts are formed；The probe adheres to slide by detecting probe information tag slot, probe adhering zone and hybridized slides
Bonding land forms, and comprising several mutually independent probe attachment regions in the probe adhering zone, probe is in the form of being lyophilized
It is attached on probe attachment region；District's groups are isolated by sample message recording areas, sample attachment region and sample in the sample attachment slide
Into sample attachment region is corresponded with probe attachment region, and sample drop is added on sample attachment region, when sample attachment region and probe are attached
When area's fitting, the sample of sample attachment region hybridizes combination with the probe realization of corresponding probe attachment region.
In said program, the probe adhering zone is a groove, and groove includes several mutually independent probe attachments
Block of cells, each independent probe attachment block of cells are made of probe attachment region and peripheral isolated area.
In said program, there is isolation ditch between each independent probe attachment block of cells.
In said program, corresponding probe on each independent probe attachment region is had recorded on the detecting probe information tag slot
Type.
In said program, slide is adhered in the surface of the hybridized slides bonding land with acrylate glue for probe
Packaging sealer and with sample attachment slide bonding.
In said program, the sample attachment region uses hydrophilic material.
In said program, the sample isolated area has hydrophobic coating.
In said program, sample information is had recorded on the sample message recording areas.
In the utility model, probe used using pipettor is applied to corresponding independence after being dissolved using vehicle composition
Probe attachment region.Specifically excipient composition composition formula is：Left-handed glucosides 0.5%, acacia 3%, mannitol 1%,
Polyacrylate 0.3%, using deionized water dissolving, 4 DEG C save backup.
For multiprobe hybridized slides described in the utility model when specifically used, each probe that slide need to be adhered in probe is attached
It area and 2 μ l hybridization buffers is added dropwise, then tip upside down on sample attachment slide on probe attachment slide, sample and probe combine,
Hybridized slides after combination are placed in 73 DEG C of denaturation 2min on metal bath, are placed in 37 DEG C of wet box hybridization 2h.
Beneficial effects of the present invention：(1) compared to traditional glass slide, the utility model can be on a hybridized slides
Preset 8 groups of probes simultaneously, and the detection of a variety of probes can be completed on same hybridized slides, enormously simplify traditional FISH
Operating procedure；(2) compared with existing all glass slides for carrying out FISH experiments, the utility model by unique design,
Reduce the usage amount of probe, reduce the use cost of probe.
Description of the drawings
Fig. 1 is that the probe of multiprobe hybridized slides described in the utility model adheres to slide plan view.
Fig. 2 is that the probe of multiprobe hybridized slides described in the utility model adheres to slide stereochemical structure and dimension data.
Fig. 3 is that the sample of multiprobe hybridized slides described in the utility model adheres to slide plan view.
Wherein, 1 is detecting probe information tag slot, and 2 be probe adhering zone, and 3 be hybridized slides bonding land, and 4 adhere to for probe
Area, 5 be peripheral isolated area, and 6 be isolation ditch, and 7 be sample message recording areas, and 8 be sample attachment region, and 9 be sample isolated area.
Specific embodiment
For a better understanding of the present invention, with reference to the embodiment content that the present invention is furture elucidated, but the present invention
Content is not limited solely to the following examples.
Such as attached drawing 1~3, a kind of multiprobe hybridized slides, by probe attachment slide (A) and sample attachment slide (B) two
Divide and form.
The probe attachment slide (A) is engaged by detecting probe information tag slot (1), probe adhering zone (2) and hybridized slides
Area (3) forms, and the probe adhering zone (2) is a groove, and groove includes several mutually independent probe attachment block of cells,
Each independent probe attachment block of cells is made of probe attachment region (4) and peripheral isolated area (5), each independent probe attachment
There is isolation ditch (6) between block of cells, probe is attached in the form of being lyophilized on probe attachment region；The detecting probe information mark
Corresponding probe species on each independent probe attachment region, the surface of the hybridized slides bonding land (3) are had recorded in area (1)
With acrylate glue, the bonding for the packaging sealer of probe attachment slide and with sample attachment slide.
The sample attachment slide (B) is by sample message recording areas (7), several mutually independent sample attachment regions (8)
It is formed with sample isolated area (9), sample drop is added on sample attachment region (8), and the sample attachment region uses hydrophilic material, described
Sample isolated area has hydrophobic coating, and sample information, sample attachment region (8) and probe are had recorded on the sample message recording areas
Attachment region (4) is in one-to-one correspondence, when sample attachment slide back-off is adhered in probe on slide, sample and the phase of sample attachment region
The probe of corresponding probe attachment region combines, and then realizes hybridization.
In the utility model, probe used using pipettor is applied to corresponding independence after being dissolved using vehicle composition
Probe attachment region.Specifically excipient composition composition formula is：Left-handed glucosides 0.5%, acacia 3%, mannitol 1%,
Polyacrylate 0.3%, using deionized water dissolving, 4 DEG C save backup.
For multiprobe hybridized slides described in the utility model when specifically used, each probe that slide need to be adhered in probe is attached
It area and 2 μ l hybridization buffers is added dropwise, then tip upside down on sample attachment slide on probe attachment slide, sample and probe combine,
Hybridized slides after combination are placed in 73 DEG C of denaturation 2min on metal bath, are placed in 37 DEG C of wet box hybridization 2h.
Obviously, above-described embodiment is only intended to clearly illustrate made example, and is not the limitation to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation or change therefore amplified
It moves within still in the protection domain of the invention.
Claims (6)
1. a kind of multiprobe hybridized slides, which is characterized in that the multiprobe hybridized slides are to adhere to slide and sample by probe
Adhere to slide two parts to form；The probe attachment slide is connect by detecting probe information tag slot, probe adhering zone and hybridized slides
District's groups are closed into the probe adhering zone is interior to include several mutually independent probe attachment regions, and probe is attached in the form of being lyophilized
It on probe attachment region；The sample attachment slide is made of sample message recording areas, sample attachment region and sample isolated area,
Sample attachment region is corresponded with probe attachment region, and sample drop is added on sample attachment region, when sample attachment region and probe adhere to
When area is bonded, the sample on sample attachment region hybridizes combination with the probe on corresponding probe attachment region.
2. multiprobe hybridized slides according to claim 1, which is characterized in that the probe adhering zone is a groove,
Groove includes several mutually independent probes attachment block of cells, each independent probe attachment block of cells by probe attachment region and
Peripheral isolated area composition.
3. multiprobe hybridized slides according to claim 2, which is characterized in that each independent probe attachment block of cells it
Between exist isolation ditch.
4. multiprobe hybridized slides according to claim 1, which is characterized in that had recorded on the detecting probe information tag slot
Corresponding probe species on each independent probe attachment region.
5. multiprobe hybridized slides according to claim 1, which is characterized in that the sample attachment region uses hydrophilic material
Matter；The sample isolated area has hydrophobic coating.
6. multiprobe hybridized slides according to claim 1, which is characterized in that had recorded on the sample message recording areas
Sample information.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483366.4U CN207537461U (en) | 2017-11-09 | 2017-11-09 | A kind of multiprobe hybridized slides |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483366.4U CN207537461U (en) | 2017-11-09 | 2017-11-09 | A kind of multiprobe hybridized slides |
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| CN207537461U true CN207537461U (en) | 2018-06-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904283A (en) * | 2017-11-09 | 2018-04-13 | 武汉康录生物技术股份有限公司 | A kind of instant multiprobe hybridized slides and its preparation method and application |
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2017
- 2017-11-09 CN CN201721483366.4U patent/CN207537461U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904283A (en) * | 2017-11-09 | 2018-04-13 | 武汉康录生物技术股份有限公司 | A kind of instant multiprobe hybridized slides and its preparation method and application |
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