CN207516234U - A kind of device of optical projection tomography - Google Patents
A kind of device of optical projection tomography Download PDFInfo
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- CN207516234U CN207516234U CN201721739161.8U CN201721739161U CN207516234U CN 207516234 U CN207516234 U CN 207516234U CN 201721739161 U CN201721739161 U CN 201721739161U CN 207516234 U CN207516234 U CN 207516234U
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Abstract
The utility model discloses a kind of device of optical projection tomography, including：Source of parallel light device, camera, telecentric imaging system, stepper motor, specimen rotating holder, computer, the computer is electrically connected with the camera, the source of parallel light device, specimen rotating holder, telecentric imaging system, camera passes sequentially through light path connection, the device utilizes camera, telecentric imaging system, stepper motor, by acquiring the photo of different focal point when rotating platform is to each focal plane, pass through the laplacian pyramid algorithm of computer again, so as to obtain long depth of field photo, filter back-projection algorithm is recycled to be rebuild, it is abundant to obtain information, the image of high resolution.It can be widely used for bio-imaging.
Description
Technical field
The invention is related to bio-imaging technical field, more particularly to a kind of device of optical projection tomography.
Background technology
Optical projection tomography (Optical Projection Tomography, OPT) technology is a kind of new three
Optical image technology is tieed up, can realize that investigation depth reaches the live body three-dimensional imaging of the toy of 10mm.
And optical projection tomography it be based on microscope camera be imaged, during micro-imaging, due to micro- system
The depth of field very little of system, to certain thickness image objects, there was only part details well focussed, other portions in obtained image
Point it is then fuzzy, this causes people that can not use the internal structure of the method for optical projection tomography clearly study sample.
For imaging system, the big depth of field means there are more clear scenery in same picture, it is meant that more can observing and controlling, prison
Control object.Therefore, the depth of field problem for increasing optical system is all the urgent problem to be solved applied to optical field all the time.
At present, mainly there is the figure of such as amplitude Qie Zhi methods, two-dimensional imaging sequence to the method for the depth of field in increase optical system
As fusion, special designing lens combination image procossing, the common lens combination image procossing (wavefront coded) with phase mask,
3-dimensional digital holography etc..Amplitude, which cuts Zhi methods, can greatly reduce imaging light energy and imaging resolution；Image co-registration method and number are complete
Breath method is required for carrying out taking longer complex calculation.
Utility model content
The purpose of this utility model is：A kind of device for expanding the optical projection tomography depth of field is provided.
The solution that the utility model solves its technical problem is：A kind of device of optical projection tomography, including：
Source of parallel light device, camera, telecentric imaging system, stepper motor, specimen rotating holder, computer, the computer and the camera
It is electrically connected, the source of parallel light device, specimen rotating holder, telecentric imaging system, camera pass sequentially through light path connection, the stepper motor
For the camera to be driven to move linearly towards biological sample, the specimen rotating holder is for driving the biological sample to rotate, institute
Camera is stated under stepper motor drive, under the rotation of specimen rotating holder, several different angles of shooting biological sample to be different
The image of focal plane, the computer are used to obtain the image of camera shooting, and will be same using laplacian pyramid algorithm
The image co-registration growth depth of field picture of angle difference focal plane, using filter back-projection algorithm by the long scape of biological sample different angle
Deep picture forms 3-D view.
Further, the specimen rotating holder is equipped with water-filled aquarium.
Further, the telecentric imaging system includes：Forward and backward lens, diaphragm, the diaphragm are located at the forward and backward lens
Between, and on the back focal plane of the front lens.
Further, the camera is COMS cameras.
The beneficial effects of the utility model are：The device utilizes camera, telecentric imaging system, stepper motor, rotating platform
By acquiring the photo of different focal point, then the laplacian pyramid algorithm by computer during to each focal plane, so as to
Go out long depth of field photo, filter back-projection algorithm recycled to be rebuild, obtain information fully, the image of high resolution.
Description of the drawings
It is required in being described below to embodiment in order to illustrate more clearly of the technical scheme in the embodiment of the utility model
Attached drawing to be used is briefly described.Obviously, described attached drawing is the part of the embodiment of the utility model rather than complete
Portion's embodiment, those skilled in the art without creative efforts, can also be obtained according to these attached drawings it
His designing scheme and attached drawing.
Fig. 1 is the structure diagram of the invention device；
Fig. 2 is the flow chart of the optical projection layer imaging method of embodiment 1.
Specific embodiment
The technique effect of the design of the utility model, concrete structure and generation is carried out below with reference to embodiment and attached drawing
It clearly and completely describes, to be completely understood by the purpose of this utility model, feature and effect.Obviously, described embodiment
It is the part of the embodiment rather than whole embodiments of the utility model, the embodiment based on the utility model, the skill of this field
The other embodiment that art personnel are obtained without creative efforts belongs to the model of the utility model protection
It encloses.In addition, all connection/connection relations being previously mentioned in text, not singly refer to component and directly connect, and refer to can be according to specific reality
Situation is applied, by adding or reducing couple auxiliary, to form more preferably coupling structure.Each technology in the invention is special
Sign, can be with combination of interactions under the premise of not conflicting conflict.
Embodiment 1, with reference to figure 1 and Fig. 2, a kind of device of optical projection tomography, including：Source of parallel light device, camera
5th, telecentric imaging system 4, stepper motor 5, specimen rotating holder 10, computer 9, the source of parallel light device is by 1 He of white light source device
Diffusion sheet 2 is formed, and the computer 9 is electrically connected with the camera 5, the source of parallel light device, specimen rotating holder 10, telecentric imaging system
System 4, camera 5 pass sequentially through light path connection, and the telecentric imaging system 4 includes：Forward and backward lens, diaphragm, the diaphragm are located at institute
Between stating forward and backward lens, and on the back focal plane of the front lens；The specimen rotating holder 10 is equipped with water-filled glass
Pond 3.The camera 5 is COMS cameras.
When the device work of the utility model, first, white light source device 1 sends out white light becomes illumination through i.e. diffusion sheet 2
Uniform parallel beam white light, is radiated on biological sample 8, since biological sample can generally be fixed using a concentration of 1% agar,
Therefore in order to reduce refractive index when light passes through agar, biological sample 8 is placed into water-filled aquarium 3, then be placed in rotation
On platform 10, carry out index matching, the forward and backward lens of the telecentric imaging system 4 all for 10 × micro- eyepiece, diaphragm position
In the back focal plane of front lens, wherein, the effect of the diaphragm of the telecentric imaging system 4 is to increase diaphragm to reduce the cone of light cone
Angle and the angle of light cone and primary optical axis increase imaging depth and reduce systematic error, and add in two thoroughly on diaphragm both sides simultaneously
The effect of mirror be front lens away from the distant of sample, picture of the sample through front lens be reduce real image, addition rear lens can will
Real image by the diminution of front lens carries out a degree of amplification.
Camera 5 is used to collect a series of project digital image and passes to computer 9.The wherein model of camera 5
Basler acA2000-340k (Base), the field depth of camera lens are 2mm, enlargement ratio 0.16, the time for exposure 0.09.
Stepper motor 6 drives camera 5 to move so that biological sample 8 and the distance of telecentric imaging system 4 change, so as to change
The initial position of the depth of field is become, often driving is primary, after opposing stationary, shoot one, finally obtains more in same angle
The photo of Zhang Butong focal planes, the present embodiment are 5, and utilize laplacian pyramid algorithm by same angle difference focal plane
Image co-registration growth depth of field picture.
Servo motor 7 puts the rotating platform 10 of biological sample 8 by driving, and rotating platform 10 often rotates 1.8 degree, carries out
The step of stating acquires the image in same angle, and rotating platform 10 1 rotates 200 times, 360 degree, then acquires figure by camera 5
Obtained project digital image is simultaneously passed to computer 9 by picture, and the co-melting conjunction long depth of field picture of 200 width, these pictures will be by computer 9
Reconstruction processing is carried out using filter back-projection algorithm, the wherein filter function of filter back-projection algorithm is S-L functions.It is obtaining
Under different rotary angle after each anatomy layer projection amount, rebuild through computer 9 according to algorithm with regard to each tomographic map of derived sample,
Its reconstructed results can be observed and be obtained from different angles the virtual sliced sheet along different directions, and then can obtain entire sample
The three-dimensional light absorbing layer analysis structure of product.Amira software process quality three-dimensional images are used again.
This method and device, by acquiring the photo of different focal point, then pass through Laplce during by each focal plane
Pyramid algorith so as to obtain long depth of field photo, recycles filter back-projection algorithm to be rebuild so that the letter that image is extracted
Breath is abundant, and image resolution ratio is high.
Wherein, the principle steps of the laplacian pyramid algorithm are：
Step 1: each source images are carried out with the grad pyramid that gradient turriform point establishes image respectively.
Step 2: pyramidal each decomposition layer carries out fusion treatment respectively to image gradient；Different decomposition layer, non-Tongfang
Fusion treatment is carried out using different fusion operators to detail pictures, finally obtains the grad pyramid of fused image.
Step 3: carrying out inverse turriform to gained grad pyramid after fusion converts (carrying out image reconstruction).
The better embodiment of the utility model is illustrated above, but the invention be not limited to it is described
Embodiment, those skilled in the art can also make various equivalent changes without departing from the spirit of the present invention
Type or replacement, these equivalent modifications or replacement are all contained in the application claim limited range.
Claims (4)
1. a kind of device of optical projection tomography, including：Source of parallel light device, camera, telecentric imaging system, feature exist
In, stepper motor, specimen rotating holder, computer are further included, the computer is electrically connected with the camera, the source of parallel light device,
Specimen rotating holder, telecentric imaging system, camera pass sequentially through light path connection, and the stepper motor is used to drive the camera towards life
Object sample moves linearly, and for the biological sample to be driven to rotate, the camera is used in stepper motor the specimen rotating holder
Under drive, under the rotation of specimen rotating holder, the image of several different angle difference focal planes of shooting biological sample, the computer
For obtaining the image of camera shooting, and laplacian pyramid algorithm is utilized by the image co-registration of same angle difference focal plane
The long depth of field picture of biological sample different angle is formed 3-D view by growth depth of field picture using filter back-projection algorithm.
2. a kind of device of optical projection tomography according to claim 1, it is characterised in that：The specimen rotating holder
It is equipped with water-filled aquarium.
3. a kind of device of optical projection tomography according to claim 2, it is characterised in that：The telecentric imaging system
System includes：Forward and backward lens, diaphragm, the diaphragm are located at the back focal plane of the front lens between the forward and backward lens
On.
4. according to a kind of device of optical projection tomography of claim 1-3 any one of them, it is characterised in that：The phase
Machine is COMS cameras.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721739161.8U CN207516234U (en) | 2017-12-12 | 2017-12-12 | A kind of device of optical projection tomography |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721739161.8U CN207516234U (en) | 2017-12-12 | 2017-12-12 | A kind of device of optical projection tomography |
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| CN207516234U true CN207516234U (en) | 2018-06-19 |
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| CN201721739161.8U Expired - Fee Related CN207516234U (en) | 2017-12-12 | 2017-12-12 | A kind of device of optical projection tomography |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108020509A (en) * | 2017-12-12 | 2018-05-11 | 佛山科学技术学院 | The method and its device of a kind of optical projection tomography |
| CN109143548A (en) * | 2018-10-11 | 2019-01-04 | 佛山科学技术学院 | A kind of long reach high-resolution image bilateral telecentric optical system |
| CN109141256A (en) * | 2018-10-23 | 2019-01-04 | 安徽农业大学 | A kind of crops Size Measuring System and method |
| CN109164558A (en) * | 2018-10-11 | 2019-01-08 | 佛山科学技术学院 | A kind of miniaturization image bilateral telecentric optical system |
| CN109164559A (en) * | 2018-10-11 | 2019-01-08 | 佛山科学技术学院 | A kind of large-numerical aperture near-infrared image bilateral telecentric optical system |
| CN109188660A (en) * | 2018-10-11 | 2019-01-11 | 佛山科学技术学院 | A kind of miniaturization object space telecentric optical system |
| CN109254383A (en) * | 2018-10-11 | 2019-01-22 | 佛山科学技术学院 | A kind of optical system of star sensor that wide spectrum is small-sized |
| CN109283658A (en) * | 2018-10-11 | 2019-01-29 | 佛山科学技术学院 | A kind of high precision small optical system of star sensor |
| CN109752377A (en) * | 2019-02-02 | 2019-05-14 | 佛山科学技术学院 | A kind of beam splitting type bimodal projection chromatography tissue blood vessel imaging device and method |
| CN112297422A (en) * | 2020-10-09 | 2021-02-02 | 南开大学 | One shot forming's 3D printing device |
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2017
- 2017-12-12 CN CN201721739161.8U patent/CN207516234U/en not_active Expired - Fee Related
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108020509A (en) * | 2017-12-12 | 2018-05-11 | 佛山科学技术学院 | The method and its device of a kind of optical projection tomography |
| CN109143548B (en) * | 2018-10-11 | 2023-11-28 | 佛山科学技术学院 | Long-working-distance high-resolution object image bilateral telecentric optical system |
| CN109164559B (en) * | 2018-10-11 | 2023-11-28 | 佛山科学技术学院 | Large-numerical aperture near-infrared object image bilateral telecentric optical system |
| CN109164558A (en) * | 2018-10-11 | 2019-01-08 | 佛山科学技术学院 | A kind of miniaturization image bilateral telecentric optical system |
| CN109164559A (en) * | 2018-10-11 | 2019-01-08 | 佛山科学技术学院 | A kind of large-numerical aperture near-infrared image bilateral telecentric optical system |
| CN109283658B (en) * | 2018-10-11 | 2023-11-28 | 佛山科学技术学院 | High-precision miniaturized star sensor optical system |
| CN109254383A (en) * | 2018-10-11 | 2019-01-22 | 佛山科学技术学院 | A kind of optical system of star sensor that wide spectrum is small-sized |
| CN109188660B (en) * | 2018-10-11 | 2023-11-28 | 佛山科学技术学院 | Miniaturized object space telecentric optical system |
| CN109283658A (en) * | 2018-10-11 | 2019-01-29 | 佛山科学技术学院 | A kind of high precision small optical system of star sensor |
| CN109188660A (en) * | 2018-10-11 | 2019-01-11 | 佛山科学技术学院 | A kind of miniaturization object space telecentric optical system |
| CN109164558B (en) * | 2018-10-11 | 2023-11-28 | 佛山科学技术学院 | Miniaturized object image bilateral telecentric optical system |
| CN109254383B (en) * | 2018-10-11 | 2023-11-28 | 佛山科学技术学院 | Wide-spectrum light and small star sensor optical system |
| CN109143548A (en) * | 2018-10-11 | 2019-01-04 | 佛山科学技术学院 | A kind of long reach high-resolution image bilateral telecentric optical system |
| CN109141256A (en) * | 2018-10-23 | 2019-01-04 | 安徽农业大学 | A kind of crops Size Measuring System and method |
| CN109752377A (en) * | 2019-02-02 | 2019-05-14 | 佛山科学技术学院 | A kind of beam splitting type bimodal projection chromatography tissue blood vessel imaging device and method |
| CN109752377B (en) * | 2019-02-02 | 2024-02-13 | 佛山科学技术学院 | Spectroscopic bimodal projection tomography tissue blood vessel imaging device and method |
| CN112297422A (en) * | 2020-10-09 | 2021-02-02 | 南开大学 | One shot forming's 3D printing device |
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