CN207512167U - A kind of collection device of liquid culture fungal mycelium - Google Patents

Abstract

The utility model discloses a kind of collection devices of liquid culture fungal mycelium.Including vacuum pump, Buchner funnel, sealing ring and bottle,suction, the outlet that the Buchner funnel includes the cylindrical portion of upper end and lower end is connected with cylindrical portion, sealing ring is cased on the outlet of the Buchner funnel, the outlet with sealing ring of Buchner funnel is inserted into the bottleneck of bottle,suction, sealing ring is adjacent to outlet on one side, another side is adjacent to the bottle mouth wall of bottle,suction, realize the sealed connection between the outlet of Buchner funnel and the bottleneck of bottle,suction, the bleeding point of the vacuum pump is connected through pipeline with the gas outlet of bottle,suction；The string bag for sticking cylindrical portion inner wall is equipped in Buchner funnel cylindrical portion, the rims of the string bag stretch out cylindrical portion opening, the steel wire for being pasted on string bag wall is equipped in the string bag.It is low etc. that the utility model collection device has the advantages that at low cost, steel wire easy to operate repeats utilization, mycelium moisture content applied widely, obtaining, is suitble to promote and apply.

Description

A kind of collection device of liquid culture fungal mycelium

Technical field

The utility model category Spawn incubation utensil technical field, and in particular to a kind of liquid culture fungal mycelium it is simple Collection device.

Background technology

Fungal studies personnel carry out fungal mycelium total DNA, RNA and protein extraction or fungi fermentation formula, In the researchs such as parameter exploration, need to carry out Liquid Culture proliferation to mycelium, then again isolate mycelium from zymotic fluid Come.

Often mycelium is isolated from zymotic fluid using supercentrifugal process and filtration method at present.For supercentrifugal process, Since the mycelium of certain fungi strain Liquid Cultures is more sticky, in suspension bulky grain state, even if the speed of centrifugation is very Height is also difficult to compactly for it to be deposited to centrifugation bottom of the tube, inferior separating effect completely, and the moisture inside mycelia ball particle is also difficult To be removed by centrifuging, and centrifuge is of high cost, cumbersome time-consuming；Filtration method is included with filter paper filtering and common gauze mistake Filter paper is filtered in filter etc., then also due to zymotic fluid concentration is high and sticky, hardly results in relatively dry mycelia, and filter paper is micro- Hole is fermented liquid and blocks soon, it is difficult to and filtering is gone down, and need to constantly replace filter paper, and adsorbs the mycelium on filter paper, due to It is easily scratched, therefore also more difficult is completely taken off after filter paper is wet；For filtered through gauze, then in the case that sample size is more, by The mycelia cross contamination that mycelia causes different sample rooms is easily stained in gauze, therefore not reproducible utilization, therefore cause unnecessary wave Take, and the few then mycelia of the gauze number of plies easily leaks out, and easily remains mycelia between the number of plies at most every layer of gauze and in gauze hole, causes sample The waste of product and the error of data.

Utility model content

The purpose of this utility model is that：Existing effect is not when existing fungal mycelium being overcome to be detached from zymotic fluid The loss of good, sample is big and the problems such as the not reproducible utilization of easy cross contamination, cumbersome time-consuming, of high cost, consumptive material, provides one kind Structurally and operationally simple, energy conservation and environmental protection and the repeatable collection device of liquid culture fungal mycelium utilized.

In order to realize above-mentioned purpose of utility model, the utility model provides a kind of collection of liquid culture fungal mycelium Device, including：Vacuum pump, Buchner funnel, sealing ring and bottle,suction, the Buchner funnel include cylindrical portion and the lower end of upper end The outlet being connected with cylindrical portion, sealing ring is cased on the outlet of the Buchner funnel, and Buchner funnel carries sealing ring Outlet be inserted into the bottleneck of bottle,suction, sealing ring is adjacent to outlet on one side, and another side is adjacent to the bottle mouth wall of bottle,suction, realizes cloth Sealed connection between the outlet of family name's funnel and the bottleneck of bottle,suction, the bleeding point of the vacuum pump is through pipeline and bottle,suction Gas outlet be connected；

The string bag for sticking cylindrical portion inner wall is equipped in Buchner funnel cylindrical portion, the rims of the string bag stretch out cylindrical portion and open Mouthful, it is equipped in the string bag and is adapted with string bag shape, be pasted on the steel wire of string bag wall, the mesh size of the steel wire is 150-250 mesh；

The top of the bottle,suction is equipped with gas outlet, and lower part is equipped with outlet tube.

A kind of improvement of collection device as the utility model liquid culture fungal mycelium is set on the outlet tube There is valve.

Relative to the prior art, the utility model is by having the advantages that：

(1) steel wire that the utility model is bonded by being set to the cylindrical portion of Buchner funnel, the mycelium collected Water content is low, and separating rate is exceedingly fast, and with utilization is repeated after aseptic water washing after steel wire use, has energy conservation and environmental protection, keeps away The advantages of exempting from cross contamination.

(2) the utility model selects steel wire of the mesh number for 150~250 mesh according to the size of mycelium pellet, adaptable The advantages of wide, suitable for separating and collecting for various fungal myceliums.

(3) the utility model in the cylindrical portion of Buchner funnel by setting the string bag, during being collected by filtration for a long time, If when blocking, which occurs, in steel wire causes the rate of filtration slack-off, can directly lifted the string bag, steel wire of being more convenient for is replaced, washing and true The mycelial collection of bacterium, improves work efficiency.

(4) the utility model in the lower part of bottle,suction by setting outlet tube, when the zymotic fluid of bottle,suction is more, without Extract Buchner funnel, you can release the zymotic fluid in bottle, the sustainable work of collection device.

Description of the drawings

With reference to the accompanying drawings and detailed description, the collection device to the utility model liquid culture fungal mycelium and Advantage is described in detail.

Fig. 1 is the structure diagram of the collection device of the utility model liquid culture fungal mycelium；

Reference sign：1- Buchner funnels；The 2- string bags；3- steel wires；4- sealing rings；5- bottle,suctions；6- vacuum pumps；7- Outlet tube；8- valves；9- gas outlets；10- cylindrical portions；11- outlets.

Specific embodiment

In order to be more clear the purpose of this utility model, technical solution and advantageous effects, with reference to embodiments, The present invention is further described in detail.It should be understood that the embodiment described in this specification is used for the purpose of Explain the utility model, be not intended to limit the utility model, parameter, the ratio of embodiment etc. can adaptation to local conditions make a choice and Substantial effect is had no to result.

Embodiment 1

Referring to Fig. 1, the collection device of the utility model liquid culture fungal mycelium, including：Vacuum pump 6, Bu Shi leakages Bucket 1, sealing ring 4 and bottle,suction 5, the outlet that the Buchner funnel includes the cylindrical portion 10 of upper end and lower end is connected with cylindrical portion 10 Pipe 11 is cased with sealing ring 4 on the outlet of the Buchner funnel, and the outlet with sealing ring of Buchner funnel 1, which is inserted into, to be taken out The bottleneck of filter flask 5, sealing ring are adjacent to outlet on one side, and another side is adjacent to the bottle mouth wall of bottle,suction 5, realizes the outlet of Buchner funnel Sealed connection between pipe and the bottleneck of bottle,suction, gas outlet 9 phase of the bleeding point through pipeline with bottle,suction of the vacuum pump Even；

The string bag 2 for sticking cylindrical portion inner wall is equipped in Buchner funnel cylindrical portion, the rims of the string bag stretch out cylindrical portion Opening, peripheral edge portion are conveniently replaceable what is carried when steel wire, are equipped in the string bag and are adapted with string bag shape, are pasted on string bag wall Steel wire 3, the mesh size of the steel wire is 150-250 mesh；

The top of the bottle,suction is equipped with gas outlet 9, and lower part is equipped with outlet tube 7, valve is provided on the outlet tube Door 8.

The collection device of the utility model liquid culture fungal mycelium works as follows：

After installation connects each component in collection device, zymotic fluid is poured into the steel wire in Buchner funnel, is connected The power supply of vacuum pump, suction filtration start.It when dripless oozes outlet most advanced of Buchner funnel again, filters and completes, respectively The fungal fermented filtrate in the fungal mycelium and bottle,suction on steel wire is collected, completes the collection of fungal mycelium.

Application experiment：

Experimental group：Mushroom, oyster mushroom and black fungus zymotic fluid are poured into the liquid culture fungal mycelium of the present embodiment respectively Collection device Buchner funnel in steel wire in, open and filter, treat the most advanced dripless again of the pars infundibularis of Buchner funnel When oozing, the collection of fungal mycelium is completed.

Separately set two groups of controls：1. carry out mycelium collection with filter paper substitution steel wire.2. using 12000 revs/min of revolution It carries out high speed centrifugation and collects mycelium.

After the mycelium of collection is weighed weight in wet base respectively, claim its dry weight again after 80 DEG C of drying, the final each method that obtains is received The mycelia body water content of collection such as table 1.

Table 1

Control group 1：High speed centrifugation collects mycelium.

Control group 2：It is filtered by vacuum using filter paper as filter membrane.

Experimental group：It is filtered by vacuum using steel wire as filter membrane.

Moisture content=(weight in wet base-dry weight)/weight in wet base × 100%.

A is represented has pole significant difference (P ＜ 0.01) compared with control group 1；B is represented to be had significantly compared with control group 1 Difference (P ＜ 0.05)；C representatives have pole significant difference compared with control group 1 and control group 2 respectively.

Control group 1 collects mycelium using supercentrifugal process, more sticky for zymotic fluid, and mycelium is difficult sedimentation, right Larger in mycelium pellet, contained liquid can not be removed, therefore the mycelium that this method is collected is aqueous by centrifugation in mycelium pellet Rate is higher, and drying takes longer and more easy to pollute in drying course.This method purchase centrifuge, the rotary head of different size, difference The centrifuge tube of specification is costly, and time-consuming, more difficult (when example weight has not balanced, high speed centrifugation is easy for operation for high speed centrifugation Damage rotary head, centrifuge tube, in some instances it may even be possible to collapse to open and hurt sb.'s feelings).

The application filter paper of control group 2 carries out vacuum filtration as filter membrane and collects mycelium, limpider for zymotic fluid, filters Effect is preferable, and can roll mycelium pellet with metal medicine spoon, and the zymotic fluid for making it internal is released suction filtration and goes down, but filter paper Micropore is smaller, easily blocks, needs to change filter paper；More sticky for zymotic fluid, filter paper blocks soon, and frequently replacing can cause Sample loss, and the mycelium collected is paste, moisture content is higher, and drying takes longer and more easy to pollute in drying course. This method needs to constantly change filter paper for certain samples, also results in certain waste.

Experimental group application steel wire carries out vacuum filtration as filter membrane and collects mycelium, since steel wire screen distance is larger, no Blocking is easily caused, aperture is unlikely to leak out mycelia again, therefore the good separating effect of mycelium and zymotic fluid, the bacterium collected Filament water content is low, and the scope of application is also relatively wide, and the fungi fermentation of different characteristics can collect to obtain the relatively low mycelium of moisture content. In addition, every square metre 200 mesh steel wires (304 food-grade steel) price is about 40 yuan, the filtering steel of 150-170 can be made Silk screen, it is at low cost and utilization can be iteratively repeated.

In conclusion experimental group application steel wire carries out vacuum filtration as filter membrane collects mycelium, the mycelium of acquisition Moisture content is low, no matter and zymotic fluid is more sticky or limpider is all suitable for.This method is simple and quick, at low cost, steel wire Utilization can be iteratively repeated, energy-saving low-carbon environmental protection is economical and practical.Therefore, the utility model device is worth of widely use.

According to the disclosure and teachings of the above specification, the utility model those skilled in the art can also be to above-mentioned reality The mode of applying carries out appropriate change and modification.Therefore, the utility model is not limited to specific implementation disclosed and described above Mode should also be as falling into the scope of the claims of the utility model to some modifications and changes of the utility model. In addition, although having used some specific terms in this specification, these terms merely for convenience of description, not to this reality With novel composition any restrictions.

Claims (2)

1. a kind of collection device of liquid culture fungal mycelium, which is characterized in that including：Vacuum pump, Buchner funnel, sealing ring And bottle,suction, the outlet that the Buchner funnel includes the cylindrical portion of upper end and lower end is connected with cylindrical portion, Bu Shi leakages Sealing ring is cased on the outlet of bucket, the outlet with sealing ring of Buchner funnel is inserted into the bottleneck of bottle,suction, sealing ring one The tight outlet of face paste, another side are adjacent to the bottle mouth wall of bottle,suction, realize between the outlet of Buchner funnel and the bottleneck of bottle,suction Sealed connection, the bleeding point of the vacuum pump is connected through pipeline with the gas outlet of bottle,suction；

The string bag for sticking cylindrical portion inner wall is equipped in Buchner funnel cylindrical portion, the rims of the string bag stretch out cylindrical portion opening, It is equipped in the string bag and is adapted with string bag shape, be pasted on the steel wire of string bag wall, the mesh size of the steel wire is 150- 250 mesh；

The top of the bottle,suction is equipped with gas outlet, and lower part is equipped with outlet tube.

2. the collection device of liquid culture fungal mycelium according to claim 1, which is characterized in that on the outlet tube It is provided with valve.