CN207477489U - A kind of near-infrared fluorescent-X ray bimodulus small animal imaging equipment - Google Patents
A kind of near-infrared fluorescent-X ray bimodulus small animal imaging equipment Download PDFInfo
- Publication number
- CN207477489U CN207477489U CN201720389989.9U CN201720389989U CN207477489U CN 207477489 U CN207477489 U CN 207477489U CN 201720389989 U CN201720389989 U CN 201720389989U CN 207477489 U CN207477489 U CN 207477489U
- Authority
- CN
- China
- Prior art keywords
- ray
- bimodulus
- small animal
- infrared fluorescent
- imaged
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model discloses near-infrared fluorescent X ray bimodulus small animal imaging equipment, the near-infrared fluorescent X ray bimodulus small animal imaging equipment is by excitation light source, x-ray source, x-ray fluorescence conversion screen, optical filter, focusing objective len, fluorescent probe, objective table, computer forms, and wherein fluorescent probe wave-length coverage covers 400 1700 nm.The excitation light source, fluorescent probe, optical filter combination, transparent objective table can be controlled by computer, and computer can also shoot and export images, and be preserved.The utility model can treat imaging object and carry out near-infrared fluorescence imaging and x-ray imaging acquisition fluorescent images, and two kinds of imaging advantages are complementary to one another.
Description
Technical field
The utility model is related to biomedical imaging technical field more particularly to a kind of near-infrared fluorescent-X ray bimodulus are small
Animal imaging equipment.
Background technology
Two area's fluorescence of near-infrared（NIR-II 1000-1700 nm）Biological tissue is greatly reduced to the absorption of photon and is dissipated
It penetrates, is interfered almost without archebiosis fluorescence, therefore compared to traditional one area's fluorescence of visible and near-infrared (400-900 nm)
Living imaging, two area's fluorescence imaging of near-infrared have better tissue penetration depths and higher spatial resolution.Near-infrared is glimmering
Photoimaging systems can carry out live body real time imagery tracer, can observe overall process of the labeled cell in body, but can not distinguish
Not in the specific position of organ, imaging depth is also limited.
X-ray fluorescence, which is imaged in the fields such as medical treatment, safety check, has very important status.Animal tissue's structure, be by
Different elements are formed, and have different density according to the size of each element amount summation in various organization unit's volumes, when intensity is equal
During the equal different densities institutional framework of even x-ray penetration thickness, due to degree of absorption difference, in X ray on piece or fluorescent screen
It shows with black and white（Or light and shade）Comparison, the X ray image of level difference.Based on these principles, X-ray imaging can differentiate animal
Each histoorgan and organ generate pathological change.But X ray does not have label and traceability.
Near-infrared fluorescent-X ray bimodulus small animal imaging equipment has merged two area's fluorescence imaging of near-infrared and x-ray imaging
The advantages of, it realizes their mutual supplement with each other's advantages, makes the imaging results of acquisition more accurate, more reliable.It is early in the early diagnosis of major disease
It controls, will play a significant role in the formulation of therapeutic scheme, the verification of therapeutic effect and assessment.
Invention content
To solve the above-mentioned problems, the utility model provides a kind of near-infrared fluorescent-X ray bimodulus small animal imaging and sets
It is standby, including：Excitation light source, x-ray source, fluorescent probe, focusing objective len, x-ray fluorescence conversion screen, optical filter, transparent load
Object platform and computer.
The excitation light source makes it send out near-infrared glimmering for irradiating object to be imaged of the excitation with near-infrared fluorescent
Light；
The x-ray source is used to generate X ray；
The fluorescent probe is for fluorescence signal of the acquisition from object to be imaged；
The focusing objective len is used to obtain the image of focal plane；
The x-ray fluorescence conversion screen is used to penetrating the ray that x-ray source is sent out into the X ray conversion after object to be imaged
For fluorescence；
The optical filter interferes near-infrared fluorescent for filtering；
The transparent objective table is used to carry object to be imaged；
The computer is used to control the output and storage of sharp each functional component and images.
Further, the near-infrared fluorescent-X ray bimodulus small animal imaging equipment, what fluorescent probe captured
Fluorescent signals wavelengths are 400-1700 nm.
On the basis of said program, the excitation light source can select suitable wavelength, light according to practical application request
The switch in source is in near-infrared fluorescent-X ray bimodulus small animal imaging equipment, but the power of light source is controlled by switching,
It can be controlled by computer terminal.
On the basis of said program, x-ray source is further provided with x-ray collimator, and x-ray source, x-ray collimator, X
Ray fluorescence conversion screen and fluorescent probe are located on same axis, and the power switch of x-ray source individually controls in equipment.
On the basis of said program, the fluorescent probe can both acquire to be imaged from fluorescent exciting source excitation
The fluorescence signal that object generates can also acquire the X ray that x-ray source is sent out and penetrate after object to be imaged by X ray conversion screen
The fluorescence signal being converted into.
On the basis of said program, the optical filter is generated for filtering fluorescent exciting source excitation object to be imaged
Fluorescence signal in interference signal.
On the basis of said program, the transparent objective table does not absorb X ray, can penetrate X ray well
The X ray that source is sent out.
On the basis of said program, the computer is used to control power, the wavelength of excitation light source；Control optical filter
Shaft is to rotate to desired optical filter；The specific location of objective table is controlled, imaging is made to reach optimum efficiency；Select detector
Time for exposure so that imaging fluorescence signals reach best；Computer can export the images of shooting, video and be deposited
Storage.
A kind of near-infrared fluorescent of the utility model-X ray bimodulus small animal imaging equipment, remarkable advantage are embodied in：
A kind of near-infrared fluorescent of the utility model-X ray bimodulus small animal imaging equipment can be in one, an instrument
Two area's fluorescence of X-ray and near-infrared is realized on image camera simultaneously（NIR-II 1000-1700 nm）X-ray and near red is realized in imaging
The mutual supplement with each other's advantages of outer 2nd area fluorescence imaging makes the imaging results of acquisition more accurate, more reliable, more comprehensively.This bimodal medicine
Image documentation equipment early controlled in the early diagnosis of major disease, it is important to be played in the formulation of therapeutic scheme, the verification of therapeutic effect and assessment
Effect.
Description of the drawings
Fig. 1 is the structure of near-infrared fluorescent-X ray bimodulus small animal imaging equipment of embodiment according to the present utility model
Schematic diagram.
Excitation light source 1, x-ray source 2, fluorescent probe 3, focusing objective len 4, transparent objective table, 5, x-ray fluorescence conversion screen
6, optical filter 7 and computer 8.
Specific embodiment
For the purpose of this utility model, technical solution and advantage is more clearly understood, below in conjunction with specific embodiment, and
With reference to attached drawing, the utility model is further described.
As shown in Figure 1, the utility model belongs to a kind of near-infrared fluorescent-X ray bimodulus small animal imaging equipment, it is described glimmering
Light imaging apparatus include excitation light source 1, x-ray source 2, fluorescent probe 3, focusing objective len 4, objective table, 5, x-ray fluorescence conversion
Screen 6, optical filter 7 and computer 8.
The excitation light source 1 is used to excite object to be imaged on objective table 4, can be according to treating different excitation wavelengths
It needs, the excitation light source in the range of unrestricted choice wavelength 700-1350nm；It can also be according to practical object difference fluorescence to be imaged
The demand of intensity adjusts the power 0-100W of excitation light source；Beam expanding lens is further included on excitation light source, for by the glimmering of excitation light source
Light is uniformly irradiated to by the set of pieces on the sample to be imaged on objective table so that the sample to be imaged is completely uniform
Excitation beam is covered, so that reaching good imaging effect.
The x-ray source 2 is further provided with x-ray collimator, and x-ray source, x-ray collimator, x-ray fluorescence conversion screen
It is located on same axis with fluorescent probe, the power switch of x-ray source individually controls in equipment；Surveying near-infrared fluorescent
When, x-ray fluorescence conversion screen can remove, and in x-ray imaging, x-ray fluorescence conversion screen is loaded on.
The fluorescent probe 3 is used to capture the fluorescence signal of object to be imaged on objective table 5, can both acquire from glimmering
The fluorescence signal that phot-luminescence source excitation object to be imaged generates, can also acquire the X ray that x-ray source is sent out penetrate it is to be imaged
The fluorescence signal being converted into after object by X ray conversion screen 5, the fluorescent signals wavelengths of fluorescent probe acquisition are 400-1700
nm。
The focusing objective len 4 is before fluorescent probe, for obtaining the image of focal plane.
The objective table 5 does not absorb X ray, can be sent out well through x-ray source for carrying object to be imaged
The X ray gone out.And mobile device of the objective table equipped with vertically and horizontally two axis, can by mobile vertically and horizontally axis come
Arbitrarily it is moved to target location.
The x-ray fluorescence conversion screen 6 is used to penetrating the ray that x-ray source is sent out into the X ray turn after object to be imaged
Fluorescence is changed to, in order to which fluorescent probe acquires signal.
The optical filter 7 is for filtering interference fluorescence, and optical filter can be selected arbitrarily as needed, including different fluorescence waves
It is long band logical, long logical and short logical.
The computer 8 is used to control power, the wavelength of excitation light source；The shaft for controlling optical filter is desired to rotate to
Optical filter；The specific location of objective table is controlled, imaging is made to reach optimum efficiency；Select the time for exposure of detector so that imaging
Fluorescence signal reaches best；Computer can export the images of shooting, video and be stored.
One typical operation of aforementioned near-infrared fluorescent-X ray bimodulus small animal imaging equipment is as follows：
Using rat be model as object to be imaged, carry out near-infrared fluorescence imaging first, fluorescent material selects transmitted wave
The silver selenide quantum dot of long 1250 nm.It is first turned on the power switch of wide spectrum imaging device；Then it selects on the controller
The long pass filter of the laser of 800 nm and 1100 nm；Mouse injecting anesthetic needle is given from tail vein, after waiting rat anesthesias
Again the silver selenide quantum dot of 100 μ L, 1 mg/mL is injected to it；Mouse is placed in center on transparent objective table, X is removed and penetrates
Line fluorescence conversion screen；It is focused again by focusing objective len to image camera, makes its imaging picture clear, extremely pass through calculating
Then the power of 2 W of software selective exitation light source, 100 ms time for exposure of fluorescent probe are shot, and preserve data.The
Two steps carry out x-ray imaging, first close near-infrared excitation light source, remove optical filter, open X ray source switch, load onto X ray
Fluorescence conversion screen selects the fluorescent probe time for exposure then to be shot, and preserve data in computer terminal by software.
During near infrared imaging pattern, the fluorescence photographed in fluorescent probe is that exciting light is irradiated to mouse by beam expanding lens
With, and penetrate mouse skin and organize to excite the fluorescence for being previously implanted internal silver selenide quantum dot and sending out 1250 nm,
The fluorescence overflows again through mouse tissue and skin and reaches fluorescent probe and obtain.
During x-ray imaging pattern, the fluorescence photographed in fluorescent probe is that X ray penetrates objective table and mouse reaches
It is converted on x-ray fluorescence conversion screen.
The various embodiments described above are merely to illustrate the utility model, wherein the structure of each component, connection mode and method are implemented
Process etc. can all be varied from, every equivalents carried out on the basis of technical solutions of the utility model and change
Into should not exclude except the scope of protection of the utility model.
Claims (8)
1. a kind of near-infrared fluorescent-X ray bimodulus small animal imaging equipment, it is characterised in that including：
Objective table is at least used to carry object to be imaged；
Excitation light source at least to generate exciting light and irradiate object to be imaged, makes object to be imaged send out near-infrared fluorescent；
X-ray source, at least for generating X ray and irradiating object to be imaged,
X-ray fluorescence conversion screen is at least converted to fluorescence will penetrate the X ray after object to be imaged；
Optical filter, at least filtering out the near-infrared fluorescent that is sent out by the object to be imaged and be converted by the x-ray fluorescence
Shield the interference near-infrared fluorescent in the fluorescence of output；
Fluorescent probe, at least acquiring the near-infrared fluorescent exported by optical filter；
And control unit, at least it is connect with excitation light source, x-ray source and fluorescent probe；
When the small animal imaging equipment works, the x-ray source, object to be imaged, x-ray fluorescence conversion screen, optical filter,
Fluorescent probe is set gradually along an axis.
2. near-infrared fluorescent according to claim 1-X ray bimodulus small animal imaging equipment, it is characterised in that：It is described to swash
The source power that shines is 0-100 W, and the wavelength of excitation light source is 700-1350 nm.
3. near-infrared fluorescent according to claim 2-X ray bimodulus small animal imaging equipment, it is characterised in that further include
Beam expanding lens between excitation light source and object to be imaged, the beam expanding lens is at least used for will be after the exciting light uniform expansion
It is irradiated on object to be imaged.
4. near-infrared fluorescent according to claim 1-X ray bimodulus small animal imaging equipment, it is characterised in that：It is described glimmering
The fluorescent signals wavelengths of optical detector acquisition are 400-1700 nm.
5. near-infrared fluorescent according to claim 1-X ray bimodulus small animal imaging equipment, it is characterised in that：The filter
The bandpass range of mating plate is 1000-1700 nm.
6. near-infrared fluorescent according to claim 1-X ray bimodulus small animal imaging equipment, it is characterised in that further include X
Ray collimator, and the x-ray source, x-ray collimator, x-ray fluorescence conversion screen are set gradually along the axis.
7. near-infrared fluorescent according to claim 1-X ray bimodulus small animal imaging equipment, it is characterised in that：The load
Object platform uses the transparent objective table passed through for X ray.
8. near-infrared fluorescent according to claim 1-X ray bimodulus small animal imaging equipment, it is characterised in that：The control
Unit processed uses computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720389989.9U CN207477489U (en) | 2017-04-14 | 2017-04-14 | A kind of near-infrared fluorescent-X ray bimodulus small animal imaging equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720389989.9U CN207477489U (en) | 2017-04-14 | 2017-04-14 | A kind of near-infrared fluorescent-X ray bimodulus small animal imaging equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207477489U true CN207477489U (en) | 2018-06-12 |
Family
ID=62483448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720389989.9U Active CN207477489U (en) | 2017-04-14 | 2017-04-14 | A kind of near-infrared fluorescent-X ray bimodulus small animal imaging equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207477489U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106963341A (en) * | 2017-04-14 | 2017-07-21 | 苏州影睿光学科技有限公司 | A kind of near-infrared fluorescent X-ray bimodulus small animal imaging equipment |
-
2017
- 2017-04-14 CN CN201720389989.9U patent/CN207477489U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106963341A (en) * | 2017-04-14 | 2017-07-21 | 苏州影睿光学科技有限公司 | A kind of near-infrared fluorescent X-ray bimodulus small animal imaging equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5305759A (en) | Examined body interior information observing apparatus by using photo-pulses controlling gains for depths | |
| JP6319448B2 (en) | Imaging device | |
| JPS639464B2 (en) | ||
| JPH10113327A (en) | Endoscope device | |
| JPWO2009028136A1 (en) | Fluorescence observation equipment | |
| DE102006004583A1 (en) | Optical scanner, to give images of marked human and animal tissue, has a distance sensor with a control unit to set the focus of the infra red light source for the image detector | |
| CN105828727A (en) | Infrared light imaging apparatus | |
| CN108371756A (en) | A kind of diagnosis and treatment one cervical lesions photodynamic therapy system | |
| CN108355252A (en) | A kind of accurate treatment system of nevus flammeus light power | |
| CN107072644A (en) | Imaging device | |
| CN110177505A (en) | Fluoroscopic imaging device and fluoroscopic imaging systems | |
| CN208756798U (en) | A kind of accurate treatment system of nevus flammeus light power | |
| CN207477489U (en) | A kind of near-infrared fluorescent-X ray bimodulus small animal imaging equipment | |
| CN106963341A (en) | A kind of near-infrared fluorescent X-ray bimodulus small animal imaging equipment | |
| JP2009257967A (en) | Fluorescence observation apparatus and fluorescence observation method | |
| US20090069695A1 (en) | Device for imaging a turbid medium | |
| CN109276230A (en) | A kind of short-wave infrared tumor imaging system and method | |
| CN206945537U (en) | A kind of wide spectrum fluorescence imaging device | |
| KR101106633B1 (en) | Dual vision optical molecular imaging device for real time fluorescence imaging of small animal | |
| JP7435272B2 (en) | Treatment support device and method of operating the treatment support device | |
| CN114869207A (en) | Electronic endoscope imaging system and method | |
| RU2596869C1 (en) | Device for fluorescent diagnosis and monitoring of photodynamic therapy | |
| TWI616190B (en) | System and operation method of acoustic-actuated optical coherence enhanced imaging lens | |
| JP2019048111A (en) | Imaging method | |
| RU2169922C1 (en) | Method and device for diagnosing proliferation areas |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant |